Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F17 I0 R1 79 154.6 3079696 97.7% 3008862 226.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,506 C→A 17.5% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,5060CA17.5% 259.7 / 12.1 105T15K (ACA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (39/44);  new base A (9/10);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206274‑3206742
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
  tgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAAAAGGAGTTGCCATAGTTGGCGAAAGAAATAAACAAAAGACAATGAAT‑AAATTA‑ACAGAGATTg                                                                                                                                                                                                                                          >  2:1499789/1‑239 (MQ=255)
              tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCCTGGTGAGGGGAAGTAATAAAAAACAAGCAGTGAAT‑AAAGTG‑TAAAAGATTGTCGCT‑AAAttt                                                                                                                                                                                                                              >  1:1231640/1‑238 (MQ=255)
              tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGAGTTTCCATGATGGGGGGAAGTAATAAGAAACAAGCAGTGAAA‑AAAATG‑ACAGAGATTGTTGCT‑TACttt                                                                                                                                                                                                                              >  1:267806/1‑238 (MQ=255)
                     cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTTAGACAAATACAAAAACCGGAGTCGCCATACTTGGCGGAAGTAATAAAAACCAAGCAGAGGAT‑AAAATG‑TAAGACATTATCCCC‑AACATT‑TCCGCCaa                                                                                                                                                                                                                     >  1:1450575/1‑238 (MQ=255)
                          aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCATACAAATACAAAAAAAGGGGTTTCCATTGTGAGGGAAAGAAAAAAAAAAAAGGAACAGAAT‑AAGATG‑ACATAGATTTTCGCT‑AACTTT‑ACCCCCCTGGttt                                                                                                                                                                                                                >  2:1435656/1‑239 (MQ=255)
                               tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACCAATACAAAAAATTGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGGCGGGAAT‑AAGATT‑ACAGAGATTGTTGGT‑CAATTT‑TACGCCATGCttttttc                                                                                                                                                                                                            >  1:732931/1‑234 (MQ=255)
                                      cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAAATTGAGGTGTCATGATGAGCGGAAATAATAAGCAAAAAGCAGTGAAT‑AAGTTG‑AAAGGGATTGTCGCT‑AAATTT‑ACCCACATGATTTTTA‑CCCGAATg                                                                                                                                                                                                    >  1:871984/1‑238 (MQ=255)
                                            tctGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑ATTTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCtgatg                                                                                                                                                                                             <  1:734121/239‑1 (MQ=255)
                                                gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAAAAAAAAATGGGGTTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAAA‑AAATTG‑AACGAGATTGTCGCG‑TACATT‑TCCCCCATTATTTTTA‑CCCCAAAGACTCATTACgt                                                                                                                                                                                         >  1:59752/1‑239 (MQ=255)
                                                 cgcAATATTAATTTTTATTTCAGCGTTGATTCACAAACTGTAAATTACATATTCAGGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgt                                                                                                                                                                                         <  2:187344/238‑1 (MQ=255)
                                                  gaaaaattcatttTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GATTTTTTCAAGACAAATACAAAAACTGGAGTTGCTATGATGAGCGAAAGTAATAAGCAACAGCCAGTAATT‑AAGTTG‑ACAGAGATTGTCGCT‑ACCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                                                        <  1:1111782/233‑1 (MQ=255)
                                                  gaaaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCAAGACAAAAACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                                                        <  1:1499789/236‑1 (MQ=255)
                                                      tatTCATTTTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAACCCACATTTTTAGTGGTATTTTGTGGCGGT‑AAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCAATGATGAGCGAAATTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATGGTCGTT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                   <  2:1225370/239‑1 (MQ=255)
                                                      tatTCATTTTTATTTTCGCTTTGATTCACGAATTTTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCAAATTTTTAGTGGTGGTTTGTGGCGGT‑GAATTTTTCCAAAAAAATAAAAAAACGGGGGTTGCCATGATGAGCAAAAGTAATAAGCAACGGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                   <  2:1231640/239‑1 (MQ=255)
                                                      tatTCATTTTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGGGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑AAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCATTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                   <  1:1435656/239‑1 (MQ=255)
                                                      tatTCATTCTTATTTGCGCGTTGATTCACAAACTGTTAATTAAATATTCACGGGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAAAACAAAAACTGGAGTTGCCATGATAAGCGAAAGTAATAAGCAACGGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                   <  1:1285598/239‑1 (MQ=255)
                                                      tatTCATTCTTAATTTCTCTGTAATCCACGAATTGTTAATTACAGATTCACGGTGGCAAAAAATATAAAACCACATTTTTGTGGGTAGTTTGTGGGGGT‑GAATTTTTCCAAACAAATACAAAAACTGGTGTTGCAATGGTGAGCGAAAATAATAAGCACCAGGCAGTGAAT‑AAGTTG‑ACATTGATTGACGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                   <  2:1495060/239‑1 (MQ=255)
                                                               ttATTTTCGCGTTGATTCACGAATTGTTAATTACATATTAACGGAGGCAAAAAAAATAAAACCACATTTGTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAAGTGGAGTTGCCATGATGAGAAAAAAAAATAAGCAACAGGCAGTAAAG‑AAGTTG‑ACAGAGATTGTCGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                          <  1:869/239‑1 (MQ=255)
                                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGGTGAGCGAAAGTAAAAAAAAACAAGCAAGGAAT‑AAAATG‑ACAGAGATTGTTGCT‑AAATTT‑ACCCCCATGATTTTTA‑TCCCAAAGGCTGATGACGGGGGGGGtaaaataaaa                                                                                                                                                                         >  1:208935/1‑239 (MQ=255)
                                                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAAAAATACAAAAAATGGGGTTTCCATGATGAGCGAAAGAAAAAAACAAAAAGCAGAGAAA‑AAAATG‑AAAGAAATTGTTGCT‑AAACTT‑TCCGCCCTGATTTTTA‑CCCCAAATCCTGATGAAGcggtggggaaaaaaaaa                                                                                                                                                                         >  1:87149/1‑228 (MQ=255)
                                                                      cgcgTTGATTCACGAACTGTTAATTACATATTCGCGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑AAATTTTTCCAGAAAAATACAAAAACTGGATTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa                                                                                                                                                                   <  2:445209/239‑1 (MQ=255)
                                                                       gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATAAAAAAAATGGAGTTGGCATGATTAGCGGAAGAAATAAACAACAAGGAGAGAAT‑AAAATG‑AGAGAGAGTGTTGCT‑AAATTT‑ACCCCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGGGGATAAAATAAAACAGCtca                                                                                                                                                                  >  1:567210/1‑237 (MQ=255)
                                                                        cgTTGATTATCAATCTGTTAATTGCTTCATAAGGGAGGAAAAAAATATAAAACCACATTTTTATTGTTAGTTGTTGGCGGT‑TAATTTTACTAAACAAATCAAAAAATTGGATTGGCCAGGATGACAGAAAGAAACAGGAAAAAGGCCGTAAAT‑AAGTTG‑CCAGAGATTGTCGCT‑ATTTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa                                                                                                                                                                 <  1:1181395/239‑1 (MQ=255)
                                                                                  cGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCAATGATGAGCAAAAGAAATAAGCAACAGGTAGTGAAT‑AAGTTG‑AAAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                                       <  1:894167/239‑1 (MQ=255)
                                                                                   gaccttttAATTACATTTTCACGGTGGAAAAAAATATAAACCCACATTTTTAGGGGTAGTTTGGGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAAT‑‑AGTTGAACAGAGATGGTCGCT‑ACCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaaa                                                                                                                                                      <  1:31673/233‑1 (MQ=255)
                                                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAAAAAAAAAAATGGGGGTGTCATGATGAGGGGAAAAAATAAACAAAAGGAAGTGAAT‑AAGTTG‑AAAGAGAATGTCGCTAAAATTT‑ACC‑CCATGATTTTTA‑CCCGAAAACCTGCTGACGTGGTGGATAAAAAAAAAAAGCTAAAAGATGCCGacaacg                                                                                                                                                   >  1:425549/1‑233 (MQ=255)
                                                                                         ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCACACAAAAACAAAAAAAGGAGTTGCCCTCATGAGGGGAAAAAAAAAAAAAAAAGCAGCGAAA‑AAGTTG‑AAAGAGATTGTGGCT‑AACTTT‑ATACCCATGATTTTTT‑ACCCAATGCCTGTTGTAGGGGTGGGGAAACCAAAAAAGATTAAAGAAGGCGaaaattgg                                                                                                                                                 >  2:819420/1‑233 (MQ=255)
                                                                                                 atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAAAAAAAACTGGAGGTGTCTTGATGAGGGGAAGTAAAAAACAAAAGGGAGAGAAT‑AAGTTG‑ACAGAGATTTTCGCT‑AACTTT‑ACCGCCCTGATTTCTT‑CCCGCATGCCTGATGACGTGGTGGATAAACTAAAACAACTAAAGAATGCCGAAACGACGTC‑GGAgggg                                                                                                                                       >  2:91766/1‑239 (MQ=255)
                                                                                                  tatgccgggTGGCAAAAAAGAAAAAACCACATTTTAAGGTGTTGTTGGTGGCGTG‑TAATTTTTCCAGACAAACACAAAAACTGGAGTTGCCATGATGAGGTAAAGAAAAAAGCAACAGGCAGTTAAA‑AGTTTG‑ACAGAGTTTGTGGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  2:903177/232‑1 (MQ=255)
                                                                                                  tatTCGCGGTGAAAAAAAATAAAAAACCAAATTTTAAGTGGTAGTTTGTGGCGGT‑GAATTTTCCAAGACAAAAAAAAAAACGGGAGTTGCCTGGATGAGCGAAAGTAATAAGCAAACGGCAGTGAAT‑AATTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  2:687815/239‑1 (MQ=255)
                                                                                                  tatTCACGGGGGCAAAAAATATAAAACAACATTTTAAGGGGTAGTTTGTGGGGGT‑GAATTTTCCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  1:323688/239‑1 (MQ=255)
                                                                                                  tatCCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGT‑AAATTTTCCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  1:813275/239‑1 (MQ=255)
                                                                                                          tgggaaaaaaaaaTAAAACCACTTTTTTAGTGGTAGTTTGGGGGGGG‑GAATTTTTACAGACAAATACAAAAACTGGAGGTGCCATGATGAGGGAAAGTAATAAGCAACAGGCATTAAAT‑AAGTTG‑ACAGAGATTTCCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  2:853033/233‑1 (MQ=255)
                                                                                                          tgggaaaaaaaTAAAAAACCCAATTTTAAGTGTGAGTTTGTGGGTGT‑AAATTTTCCCAGACAAATAAAAAACCGGGAGTTCCCTGGAGGAGGGAAATTAATAAGCAAAAGACATTAAAG‑AAGTGG‑ACAGAGATTGTCGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  2:513031/233‑1 (MQ=255)
                                                                                                          ggggaaaaaaaTATAAAACCACATTTTTGGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTGG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  2:267806/233‑1 (MQ=255)
                                                                                                          gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGGGTTGCCATGATGAGCGGAAGTAATAAAAAACAAGCAGAGAAT‑AAATTG‑ACAGAGATTGTCGCT‑AACTTT‑TCCGCCATGATTTTTA‑CCCCAAAGCCCGATGACGGGGGGGGTAAAATAAAACAGCTAAAAGATGTCGCAAAGTCGCC‑GGGGGGGGAAATTa                                                                                                                                >  1:513765/1‑237 (MQ=255)
                                                                                                          gTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGT‑GAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                                               <  1:1389957/238‑1 (MQ=255)
                                                                                                           tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAACTGGAGTTTCCATGATGAGCGAAAGTAAAAAACAACAGGCAAAGAATAAAGTTG‑‑CAGAGATTGTCGCT‑AAATTT‑ACCGCCATGATTTTTT‑CCCCAATGCCCGATGAAGTTGGGGGTAAAATAAAAAAACTAAAAGGTGCCGAAAACGCGTC‑GGTGGGGGAAAATATCt                                                                                                                             >  2:224749/1‑239 (MQ=255)
                                                                                                           tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATGATGAGCGGAAATAATAAACAAAAAGCAAGGAAT‑AAAATG‑ACAGAGATTGTTGCT‑AACTTT‑ACCGCCATGATTTTTACCCCCAATG‑CTTATGGCGGGGGGGGTAAAACAAAACAGCTAAAAGGAGCCGAAAAGGCCTCGGAGGGGGAAAAtatat                                                                                                                              >  2:628479/1‑235 (MQ=255)
                                                                                                            agaaaaaaaTATAAACCCACATTTTTAGTGGTAGTTTGGGGCGGT‑AAATTTTTCAAAACAAAAACAAAAACTGGAGTTGCCATGATGAGGAAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGTT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTa                                                                                                                            <  2:348892/236‑1 (MQ=255)
                                                                                                             gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAATAAAAAAAATGGAGTTGGCATGATTAGCGAAAGGAATAAAAAAAAGGCAGGGAAT‑AAATTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCCTGATTTTTA‑CCCCAAAACCTGATGAAGTGGGGGGTAAAATAAAACAACTAAAAGAAGCCCAAAACTCGTC‑GGTGGGGGAAAATATTTa                                                                                                                            >  1:405616/1‑238 (MQ=255)
                                                                                                              cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAGTAAAAAACAAAAAGCAGAGAAT‑AAAATG‑ACAAAGATTGTCGGC‑AACTTT‑TACACCCTGATTTTTT‑CCCCGATGGCTCATGAAGTGGGGGGTAAAATAAAAAAACGAAAAGAGACCGAAAAGTCGCC‑GGTGGGGGAAATTATATAcc                                                                                                                          >  2:1389957/1‑239 (MQ=255)
                                                                                                                 aaaaTATAAACCCAAATTTTAAGTGGTAGTTTGGGGCGGT‑AAATTTTCCCAAAAAAATAAAAAAACTGGATTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATa                                                                                                                       <  1:303345/239‑1 (MQ=255)
                                                                                                                   aaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                                                     <  1:856817/239‑1 (MQ=255)
                                                                                                                   aaTAAAAAACCACAGTTATAGTGGTAGTTTGTGGCGGT‑AAATTTGCCAAGAAAAAAACAAAAACGGGAGTTGCAATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCTCA‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                                                     <  2:732931/239‑1 (MQ=255)
                                                                                                                      ataAACCCAAATTTTGAGGGGTATTGTGTGGGGGT‑GAATTTTGCCAAAAAAATACAAAAACTGGATTTGCTATAAGGAGCGAAAGTAAAAAACAACAGGCAGTGAAA‑AAGTTG‑ACAGAGATTGTCGAT‑ACCTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATg                                                                                                                  <  2:1296097/239‑1 (MQ=255)
                                                                                                                       taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCC‑GACAAAAAAAAAAAATGGAGTTGGCATGTTGGGGGGAAAAAATAAACAAAAAGCAGTGGAT‑AAAATT‑AAAGAAAATGTTGCT‑AAATTTAACCCCCAT‑ATTTTTA‑CCCCAATGCCCGATGACGGGGGGGGGAAAAAAAAAAAGCTAAAAGATGGCGGAACATCGGC‑GGTGGGGGAAAATATATAACATAcaaagt                                                                                                                 >  2:837292/1‑234 (MQ=255)
                                                                                                                               caTTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCATGATGAGGGAAAGAAATAAGCAACAGGCAGTGAAA‑AAATTG‑AAAGAGATTGTCTCT‑AACTTT‑ACCGCCCTGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGGTAAAATAAAAAAGCTAAAGGATGCCGAAAAGTCGTC‑GGTGGGGAAAATTATATAACATCAGATTTTCGacaac                                                                                                         >  1:1188013/1‑239 (MQ=255)
                                                                                                                                        tgggattttGTGGGGGT‑GAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                                                 <  2:712410/232‑1 (MQ=255)
                                                                                                                                            ggttggggggggT‑AAATTTTACAAAAAAAATAAAAAACCTGGAGTTGCAATGATGAGCGAAATAAATAAGCACAAGGCAGTAAAT‑AAGTTG‑ACAGAGTTTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                            <  2:425549/229‑1 (MQ=255)
                                                                                                                                            aGTGTGTGGTGGT‑GAATTTTTCCAAAAAAATACAAAAACTGGAGTTGCTATGATGAGCGAAAGTAATAAGCAACGGGAAGGTAAT‑AAGTTG‑AAATAGATTGTCGTT‑CACTTT‑TCCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                            <  1:779026/239‑1 (MQ=255)
                                                                                                                                                  gtgcatgtaaaTTTTACCAAACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTATAAAGCAACAGGCAGTAAAT‑AAGTTG‑AGAAAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                                       <  1:382967/230‑1 (MQ=255)
                                                                                                                                                               ttccaaGAAAAATACAAAAATTGAAGTTGCAATGAGGGGAGAAAGAAAAAACCAACAGGCAGTGAAT‑AATTTG‑ACAGAGATTGTCCAT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          <  1:579119/234‑1 (MQ=255)
                                                                                                                                                                     gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                                                    >  2:679607/1‑239 (MQ=255)
                                                                                                                                                                      aaaaaaaaaaaaaCTGGAGTTGCAATGATGAGCAAAAGTAAAAAGCAAAAGGAAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc                                                                            <  2:1222728/222‑1 (MQ=255)
                                                                                                                                                                              aaaaaaTGGAGTTGCCATGATGAGCTAAAGTAATAAGCAACAGGAGGTGAAT‑AAGTTG‑ACAGATATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                                           <  2:82481/239‑1 (MQ=255)
                                                                                                                                                                                aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                                                  >  1:1222728/1‑230 (MQ=255)
                                                                                                                                                                                aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                         >  1:951176/1‑239 (MQ=255)
                                                                                                                                                                                aaaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                                         >  1:819506/1‑239 (MQ=255)
                                                                                                                                                                                 aaCTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGAT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:1465131/239‑1 (MQ=255)
                                                                                                                                                                                 aaCTGGAGTTGCCAGGAGGAGCGAAAGAAAAAAGCAACAGGCAGTGAAT‑AAGTTG‑AAAGAGATGGCCGCT‑AACTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  1:454149/239‑1 (MQ=255)
                                                                                                                                                                                 aaCGGGAGTTGCAATGAGAAGGAAAAGTAATAAGCAACAGGCAGGGAAT‑AAGTTA‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                        <  2:513765/239‑1 (MQ=255)
                                                                                                                                                                                  aCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCGTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                                       >  1:347329/1‑239 (MQ=255)
                                                                                                                                                                                  aCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                                       >  1:1512087/1‑239 (MQ=255)
                                                                                                                                                                                           gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCACAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                               >  2:93872/1‑238 (MQ=255)
                                                                                                                                                                                           gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                               >  1:1294000/1‑238 (MQ=255)
                                                                                                                                                                                           gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                               >  2:1114524/1‑238 (MQ=255)
                                                                                                                                                                                           gCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGTT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                                              <  1:463405/239‑1 (MQ=255)
                                                                                                                                                                                             cATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttct                                            <  1:837292/239‑1 (MQ=255)
                                                                                                                                                                                                      cGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:961929/1‑239 (MQ=255)
                                                                                                                                                                                                      cGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:574027/1‑239 (MQ=255)
                                                                                                                                                                                                      cGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg                                   >  1:175359/1‑239 (MQ=255)
                                                                                                                                                                                                       aaaaGTAAAAAGAAACAGGCAGTGAAT‑AAGTTG‑AAGGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa                                    <  2:405616/236‑1 (MQ=255)
                                                                                                                                                                                                          aGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg                               >  1:958413/1‑239 (MQ=255)
                                                                                                                                                                                                                aaGCAACAGGCAGGAAAA‑AAGTTG‑AAAGATATTGCCGCT‑AAATTC‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg                         <  2:306282/239‑1 (MQ=255)
                                                                                                                                                                                                                    aaCAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑CCCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCt                       <  1:91766/237‑1 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          >  2:1010147/1‑184 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGCGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  1:303793/1‑239 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                   >  2:264252/1‑207 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:918417/1‑239 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:1367610/1‑239 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                   >  2:172669/1‑239 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATACCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGCGGAGATTATGTTCTTCGTTACAGTCGGTTCCCGCTTccc                   >  1:1526525/1‑239 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AGCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                          <  1:1010147/184‑1 (MQ=255)
                                                                                                                                                                                                                      cAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                   <  1:264252/207‑1 (MQ=255)
                                                                                                                                                                                                                       gggaatagAAT‑AAGTTG‑AAAGAGAGTGCCACT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa                  <  1:864913/232‑1 (MQ=255)
                                                                                                                                                                                                                           aGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaa                                                                                                   >  1:1438642/1‑154 (MQ=255)
                                                                                                                                                                                                                           aGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACt                >  1:98148/1‑237 (MQ=255)
                                                                                                                                                                                                                                 t‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  1:845919/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aaggTTG‑AAGGAGTTTGTCGCT‑ATGTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg        <  2:322038/236‑1 (MQ=255)
                                                                                                                                                                                                                                    atttG‑AAAAAGTTTGTCGTT‑AAGTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg      <  2:958413/237‑1 (MQ=255)
                                                                                                                                                                                                                                    aggtG‑ACAGATATTGTCGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg      <  2:548462/236‑1 (MQ=255)
                                                                                                                                                                                                                                        g‑ACAGAGTTGGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  <  1:766642/239‑1 (MQ=255)
                                                                                                                                                                                                                                        g‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:257644/1‑239 (MQ=255)
                                                                                                                                                                                                                                        g‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:1399969/1‑239 (MQ=255)
                                                                                                                                                                                                                                        g‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  1:1185212/1‑239 (MQ=255)
                                                                                                                                                                                                                                        g‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGACTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCTCACTGCTTGGCGAGCTGc  >  1:1132289/1‑239 (MQ=255)
                                                                                                                                                                                                                                        g‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGGGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc  >  2:1261735/1‑239 (MQ=255)
                                                                                                                                                                                                                                           cAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      >  2:440884/1‑105 (MQ=255)
                                                                                                                                                                                                                                           cAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                                                      <  1:440884/105‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                
TGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGC  >  NC_000913/3206274‑3206742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A