Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F36 I0 R1 111 89.1 3136336 98.1% 3076745 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,623 G→A 33.3% W239* (TGG→TGA)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,6230GA33.3% 99.3 / 68.8 81W239* (TGG→TGA) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (27/27);  new base A (16/11);  total (43/38)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGG  >  NC_000913/756475‑756771
                                                                                                                                                    |                                                                                                                                                    
tGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTgg                                                                                                                                                      <  7:24867/149‑1 (MQ=255)
aggTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTAATTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTgg                                                                                                                                                      >  2:65522/2‑149 (MQ=255)
  gTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTgg                                                                                                                                                      >  8:324239/1‑147 (MQ=255)
  gTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCa                                                                                                                                                    <  4:17640/149‑1 (MQ=255)
   tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAg                                                                                                                                                   >  8:54777/1‑149 (MQ=255)
   tttATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAg                                                                                                                                                   >  2:342231/1‑149 (MQ=255)
     tATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGt                                                                                                                                                  <  2:212115/148‑1 (MQ=255)
       tttCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTc                                                                                                                                                <  5:17679/148‑1 (MQ=255)
       tttCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTcc                                                                                                                                               <  4:290292/149‑1 (MQ=255)
       tttCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTc                                                                                                                                                >  1:99988/1‑148 (MQ=255)
            aaGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCCCCACCAACGCCCACATTAACACCGTCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCg                                                                                                                                          >  6:289768/1‑149 (MQ=255)
                     cTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCAtt                                                                                                                                 <  6:82931/149‑1 (MQ=255)
                      tACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTg                                                                                                                                >  7:330879/1‑149 (MQ=255)
                        ccGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGcc                                                                                                                              >  6:139384/1‑149 (MQ=255)
                               ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCgg                                                                                                                       >  6:129081/1‑149 (MQ=255)
                               ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCgg                                                                                                                       <  3:287181/149‑1 (MQ=255)
                               ggCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCgg                                                                                                                       <  7:12452/149‑1 (MQ=255)
                                gCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCggg                                                                                                                      >  3:195014/1‑149 (MQ=255)
                                    cTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTa                                                                                                                  >  6:9365/1‑149 (MQ=255)
                                       gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTAc                                                                                                                 <  3:64022/147‑1 (MQ=255)
                                       gCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTAc                                                                                                                 <  5:125921/147‑1 (MQ=255)
                                                 gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAg                                                                                                     <  1:65522/149‑1 (MQ=255)
                                                 gCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAg                                                                                                     <  5:9365/149‑1 (MQ=255)
                                                   gTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGt                                                                                                   >  5:254202/1‑149 (MQ=255)
                                                    tATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGtt                                                                                                  <  3:170568/149‑1 (MQ=255)
                                                          tCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGGTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtg                                                                                            <  6:148708/149‑1 (MQ=255)
                                                            aGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                          >  7:295277/1‑149 (MQ=255)
                                                            aGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                          >  4:184800/1‑149 (MQ=255)
                                                             gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                          >  2:340577/1‑148 (MQ=255)
                                                             gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                          >  6:92838/1‑148 (MQ=255)
                                                             gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                          >  5:289592/1‑148 (MQ=255)
                                                             gTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggt                                                                                          >  7:352860/1‑148 (MQ=255)
                                                              tCCACCACCAACGCCCACATTAACACCGGCGACGGTGTAGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCAACCCGACCGGAATTGCCGCTGCGGGCGTACTGGTCACCGAAGGTTGCCgtggtg                                                                                         >  6:280138/1‑148 (MQ=255)
                                                                caccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGGGGTGaa                                                                                       <  4:138867/148‑1 (MQ=255)
                                                                caccaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCAGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAg                                                                                      >  1:190132/1‑149 (MQ=255)
                                                                 accaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAgg                                                                                     <  4:195014/149‑1 (MQ=255)
                                                                 accaccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAgg                                                                                     >  2:376473/1‑149 (MQ=255)
                                                                   caccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCAc                                                                                                          <  6:268110/126‑1 (MQ=255)
                                                                   caccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCAc                                                                                                          >  5:268110/1‑126 (MQ=255)
                                                                   caccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCCTGCCGGAGTACCGGGGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCg                                                                                   <  5:67779/149‑1 (MQ=255)
                                                                     ccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCAtt                                                                                                                                 <  4:10510/101‑1 (MQ=255)
                                                                     ccaACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCAtt                                                                                                                                 >  3:10510/1‑101 (MQ=255)
                                                                      caACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGtt                                                                                <  1:342231/149‑1 (MQ=255)
                                                                      caACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGt                                                                                 >  7:10258/1‑148 (MQ=255)
                                                                        aCGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCgg                                                                                                                                     >  1:41903/1‑94 (MQ=255)
                                                                        aCGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCgg                                                                                                                                     <  2:41903/94‑1 (MQ=255)
                                                                            ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                          >  5:32224/1‑149 (MQ=255)
                                                                            ccACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                          <  8:295277/149‑1 (MQ=255)
                                                                             cacaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgct                                                                         <  2:334916/149‑1 (MQ=255)
                                                                              acaTTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgct                                                                         >  7:67515/1‑148 (MQ=255)
                                                                                    acacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCgg                                                                                  >  2:281112/1‑133 (MQ=255)
                                                                                     cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                          <  1:283593/140‑1 (MQ=255)
                                                                                     cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATctgc                                                                          >  2:283593/1‑140 (MQ=255)
                                                                                     cacCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaac                                                                 <  2:99988/149‑1 (MQ=255)
                                                                                      cccGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaaca                                                                <  6:223143/148‑1 (MQ=255)
                                                                                      acCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTCCCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGaacaaaca                                                                >  7:190464/1‑149 (MQ=255)
                                                                                        cGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATg                                                              <  5:129081/149‑1 (MQ=255)
                                                                                        cGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATg                                                              >  1:96118/1‑149 (MQ=255)
                                                                                         ggCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATgg                                                             <  1:376473/149‑1 (MQ=255)
                                                                                                   tctgCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGTGAGTACTGTTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGtttt                                                   <  5:280138/146‑1 (MQ=255)
                                                                                                      gCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATg                                                <  5:139384/149‑1 (MQ=255)
                                                                                                      gCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATg                                                >  4:129837/1‑149 (MQ=255)
                                                                                                           gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGc                                            <  6:32224/148‑1 (MQ=255)
                                                                                                           gCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCg                                           <  3:129837/149‑1 (MQ=255)
                                                                                                              aTCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTa                                        >  5:239505/1‑149 (MQ=255)
                                                                                                                ccGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATg                                      >  6:267152/1‑149 (MQ=255)
                                                                                                                 cGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgc                                     <  6:254202/149‑1 (MQ=255)
                                                                                                                  gTGCCGGCGTACCGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATgcg                                    >  7:43613/1‑149 (MQ=255)
                                                                                                                           tACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGcc                           <  8:330879/149‑1 (MQ=255)
                                                                                                                              cGGTGCAGGATATGGAAATGTGACAGTTCCACCCGACCAGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCaaa                        <  2:190132/149‑1 (MQ=255)
                                                                                                                                 tGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGAc                     <  6:239505/149‑1 (MQ=255)
                                                                                                                                     ggATATGGAAATGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGACCGCCAAAGACCTgg                 >  1:352882/1‑149 (MQ=255)
                                                                                                                                        tatGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGGACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCgg              >  3:110455/1‑149 (MQ=255)
                                                                                                                                        tatGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCgg              >  2:238733/1‑149 (MQ=255)
                                                                                                                                        tatGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGGTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCgg              >  3:228410/1‑149 (MQ=255)
                                                                                                                                        tatGGAAATGTGGCAGTTCCACCCGACCGGCATAGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCgg              >  3:233435/1‑149 (MQ=255)
                                                                                                                                            gAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGGACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGcc           <  4:110455/148‑1 (MQ=255)
                                                                                                                                            gAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGcc           <  7:324239/148‑1 (MQ=255)
                                                                                                                                             aaaTGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGCGCGTTATGCGCCGAACGCCAAAGCCCTGGCGGGCCGt         >  7:135315/1‑149 (MQ=255)
                                                                                                                                              aaTGTGACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTg        >  5:39268/1‑149 (MQ=255)
                                                                                                                                                 gtgGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACg     >  6:251456/1‑149 (MQ=255)
                                                                                                                                                 gtgACAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCggggcg     >  7:386036/1‑144 (MQ=255)
                                                                                                                                                  tgGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGt    <  6:289592/149‑1 (MQ=255)
                                                                                                                                                  tgGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGt    >  8:91487/1‑149 (MQ=255)
                                                                                                                                                    gCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTgg  <  3:184800/149‑1 (MQ=255)
                                                                                                                                                    gCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTgg  <  5:289768/149‑1 (MQ=255)
                                                                                                                                                    gCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTgg  >  8:314574/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGTTTATTTCAAAGCCCGCGCTACCGTGCTGGCGACTGGCGGAGCAGGGCGTATTTATCAGTCCACCACCAACGCCCACATTAACACCGGCGACGGTGTCGGCATGGCTATCCGTGCCGGCGTACCGGTGCAGGATATGGAAATGTGGCAGTTCCACCCGACCGGCATTGCCGGTGCGGGCGTACTGGTCACCGAAGGTTGCCGTGGTGAAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGG  >  NC_000913/756475‑756771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A