Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,003	A→C	E128A (GAG→GCG)	yabM →	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,003	0	A	C	99.7%	939.5 / NA	295	E128A (GAG→GCG)	yabM	putative transport protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (168/126); total (168/127)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

CATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGAGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTC  >  NC_000913/77913‑78092

cATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGgcgc                                                                                         >  1:406283/1‑93 (MQ=255)

cATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGgcgc                                                                                         >  2:4688337/1‑93 (MQ=255)

 aTTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGcgcg                                                                                        >  1:1232181/1‑93 (MQ=255)

 aTTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGcgcg                                                                                        >  2:5822935/1‑93 (MQ=255)

  tttAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCgg                                                                                       <  1:7920742/93‑1 (MQ=255)

   ttAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCggg                                                                                      <  1:5417220/93‑1 (MQ=255)

   ttAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCggg                                                                                      >  2:1528590/1‑93 (MQ=255)

   ttAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCggg                                                                                      >  1:3291608/1‑93 (MQ=255)

   ttAATCGTCATTATCTGACGCTTATCACCTGTGGGGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCggg                                                                                      >  1:2655701/1‑93 (MQ=255)

     aaTCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGaa                                                                                    >  1:6541204/1‑93 (MQ=255)

      aTCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAat                                                                                   >  1:3838819/1‑93 (MQ=255)

       tCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAata                                                                                  >  2:6882970/1‑93 (MQ=255)

         gTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATg                                                                                >  1:6054131/1‑93 (MQ=255)

          tCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGc                                                                               >  1:1420164/1‑93 (MQ=255)

          tCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGAATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGc                                                                               <  2:1056365/93‑1 (MQ=255)

            aTTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCgg                                                                             <  2:4121645/93‑1 (MQ=255)

              tATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGAt                                                                           >  2:6574336/1‑93 (MQ=255)

                tCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACTGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATaa                                                                         >  2:352232/1‑93 (MQ=255)

                tCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATaa                                                                         >  2:3731502/1‑93 (MQ=255)

                 cTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAAc                                                                        >  2:3642971/1‑93 (MQ=255)

                 cTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATACGCGGATAAc                                                                        <  1:7797349/93‑1 (MQ=255)

                  tGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACt                                                                       <  2:1653323/93‑1 (MQ=255)

                  tGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACt                                                                       <  2:6980896/93‑1 (MQ=255)

                  tGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACt                                                                       >  1:3001346/1‑93 (MQ=255)

                     cGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCgg                                                                    <  1:1733778/93‑1 (MQ=255)

                     cGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCgg                                                                    >  2:2423515/1‑93 (MQ=255)

                     cGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCgg                                                                    <  1:1831387/93‑1 (MQ=255)

                      gCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgc                                                                   <  2:2994497/93‑1 (MQ=255)

                       cTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGgcg                                                                  >  1:1293904/1‑93 (MQ=255)

                             aCCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGaa                                                            <  1:4353342/93‑1 (MQ=255)

                               cTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgg                                                          >  2:6100062/1‑92 (MQ=255)

                                tgtgGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtg                                                         >  2:880505/1‑93 (MQ=255)

                                 gtggtgTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtgg                                                        >  1:5164801/1‑93 (MQ=255)

                                  tggtgTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtggt                                                       <  2:1758608/93‑1 (MQ=255)

                                  tggtgTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAgtggt                                                       <  1:7802302/93‑1 (MQ=255)

                                    gtgtGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa                                                     <  2:3299914/93‑1 (MQ=255)

                                    gtgtGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGa                                                     <  2:1414059/93‑1 (MQ=255)

                                     tgtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGAt                                                    >  1:1673672/1‑93 (MQ=255)

                                      gtgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATg                                                   <  1:6977743/93‑1 (MQ=255)

                                       tgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGt                                                  >  2:5694151/1‑93 (MQ=255)

                                       tgCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGt                                                  >  1:6624880/1‑93 (MQ=255)

                                        gCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGtt                                                 >  1:5741304/1‑93 (MQ=255)

                                          ttCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTa                                               >  1:468312/1‑93 (MQ=255)

                                          ttCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTa                                               <  1:2836332/93‑1 (MQ=255)

                                           tCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAg                                              <  1:3476594/93‑1 (MQ=255)

                                             tGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCt                                            >  2:7692726/1‑93 (MQ=255)

                                              ggCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTc                                           <  1:2860679/93‑1 (MQ=255)

                                              ggCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTc                                           <  2:3191694/93‑1 (MQ=255)

                                                cATCTCTGGCCAATACGGCAATTCCACAGTTATTTGCTCCGGCTTGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg                                         >  2:7002884/1‑93 (MQ=255)

                                                cATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg                                         >  2:5300674/1‑93 (MQ=255)

                                                cATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCgg                                         <  1:2755321/93‑1 (MQ=255)

                                                  tctcTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTg                                       >  1:3653256/1‑93 (MQ=255)

                                                   ctctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCTGTGa                                      <  2:5002142/93‑1 (MQ=255)

                                                   ctctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGa                                      <  1:1060389/93‑1 (MQ=255)

                                                   ctctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGa                                      <  2:5229642/93‑1 (MQ=255)

                                                   ctctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGa                                      <  2:383966/93‑1 (MQ=255)

                                                   ctctGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGa                                      <  2:2091864/93‑1 (MQ=255)

                                                        gCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgt                                 >  1:5066440/1‑93 (MQ=255)

                                                        gCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgt                                 <  2:2856952/93‑1 (MQ=255)

                                                        gCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgt                                 <  1:1913144/93‑1 (MQ=255)

                                                          cAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgtgc                               >  1:3237598/1‑93 (MQ=255)

                                                           aaTACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAtgcgtgcg                              >  2:6383915/1‑93 (MQ=255)

                                                            aTACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTgcgc                             >  2:6300592/1‑93 (MQ=255)

                                                            aTACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTgcgc                             >  2:1588416/1‑93 (MQ=255)

                                                             tACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCa                            <  1:4857202/93‑1 (MQ=255)

                                                             tACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCa                            >  2:4542001/1‑93 (MQ=255)

                                                             tACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCa                            >  2:2872015/1‑93 (MQ=255)

                                                             tACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCa                            >  2:7852963/1‑93 (MQ=255)

                                                               cGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGc                          <  2:7334028/93‑1 (MQ=255)

                                                                 gCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCtt                        <  1:9820/93‑1 (MQ=255)

                                                                  cAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCttt                       <  2:3408009/93‑1 (MQ=255)

                                                                  cAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCttt                       <  2:457202/93‑1 (MQ=255)

                                                                   aaTGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTtc                      >  1:4463502/1‑93 (MQ=255)

                                                                       ccACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTg                  >  1:639996/1‑93 (MQ=255)

                                                                       ccACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTg                  <  2:7511621/93‑1 (MQ=255)

                                                                       ccACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTg                  <  2:4769089/93‑1 (MQ=255)

                                                                       ccACAGATTTTTGCTCTGGCGCGGGAATATGCGGATAACTCCGCGCGCGAAGCGGCGAGGATTAGCTCGGTGATGCGTCCGCCGCCTtctccg                  >  2:1484428/1‑91 (MQ=255)

                                                                        cacaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTgg                 >  1:3440406/1‑93 (MQ=255)

                                                                         acaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGc                >  1:7224505/1‑93 (MQ=255)

                                                                         acaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGc                >  2:305332/1‑93 (MQ=255)

                                                                         acaGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGc                >  2:6222961/1‑93 (MQ=255)

                                                                           aGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCAt              >  2:3416093/1‑93 (MQ=255)

                                                                             ttATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATgg            <  1:6260833/93‑1 (MQ=255)

                                                                              tATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATggg           >  1:2010606/1‑93 (MQ=255)

                                                                              tATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATggg           >  1:3773259/1‑93 (MQ=255)

                                                                               aTTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGt          <  2:7168197/93‑1 (MQ=255)

                                                                               aTTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGt          <  1:7501174/93‑1 (MQ=255)

                                                                                 ttGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTa        <  1:6751601/93‑1 (MQ=255)

                                                                                 ttGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTa        <  1:7609106/93‑1 (MQ=255)

                                                                                 ttGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTa        <  2:2524451/93‑1 (MQ=255)

                                                                                  tGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTAt       <  1:2107899/93‑1 (MQ=255)

                                                                                   gCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATc      >  1:552802/1‑93 (MQ=255)

                                                                                   gCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATc      <  2:2704615/93‑1 (MQ=255)

                                                                                    ctctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCg     >  2:5362256/1‑93 (MQ=255)

                                                                                    ctctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCg     <  1:3948986/93‑1 (MQ=255)

                                                                                     tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCgg    <  1:2322106/93‑1 (MQ=255)

                                                                                     tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCgg    >  2:5188885/1‑93 (MQ=255)

                                                                                     tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCgg    >  2:1311791/1‑93 (MQ=255)

                                                                                     tctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCgg    >  2:1732800/1‑93 (MQ=255)

                                                                                      ctGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGt   >  2:5605674/1‑93 (MQ=255)

CATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGAGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTC  >  NC_000913/77913‑78092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGAGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACC  >  NC_000913/77910‑78096

TTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGC                                                                                               >  SRR4302543.5614441/1‑94 (MQ=60)

 TGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCG                                                                                              >  SRR4302543.652161/1‑94 (MQ=60)

 TGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCG                                                                                              <  SRR4302543.2837768/94‑1 (MQ=60)

 TGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCG                                                                                              >  SRR4302543.1561025/1‑94 (MQ=60)

  GCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGC                                                                                             >  SRR4302543.410030/1‑94 (MQ=60)

  GCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGC                                                                                             >  SRR4302543.2935675/1‑94 (MQ=60)

   CATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCG                                                                                            >  SRR4302543.1243277/1‑94 (MQ=60)

   CATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCG                                                                                            >  SRR4302543.1999045/1‑94 (MQ=60)

    ATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGG                                                                                           >  SRR4302543.3865992/1‑94 (MQ=60)

     TTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGG                                                                                          >  SRR4302543.99207/1‑94 (MQ=60)

     TTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGG                                                                                          <  SRR4302543.7995144/94‑1 (MQ=60)

     TTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGG                                                                                          >  SRR4302543.3321315/1‑94 (MQ=60)

     TTTAATCGTCATTATCTGACGCTTATCACCTGTGGGGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGG                                                                                          >  SRR4302543.2679779/1‑94 (MQ=60)

      TTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGA                                                                                         <  SRR4302543.6639426/94‑1 (MQ=60)

      TTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGA                                                                                         <  SRR4302543.5466495/94‑1 (MQ=60)

       TAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAA                                                                                        >  SRR4302543.6602772/1‑94 (MQ=60)

        AATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAAT                                                                                       <  SRR4302543.6961859/94‑1 (MQ=60)

        AATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAAT                                                                                       >  SRR4302543.3872699/1‑94 (MQ=60)

         ATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATA                                                                                      >  SRR4302543.2161600/1‑94 (MQ=60)

          TCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATAT                                                                                     <  SRR4302543.5909181/94‑1 (MQ=60)

          TCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATAT                                                                                     <  SRR4302543.5159639/94‑1 (MQ=60)

           CGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATG                                                                                    >  SRR4302543.6111442/1‑94 (MQ=60)

           CGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATG                                                                                    >  SRR4302543.6111443/1‑94 (MQ=60)

           CGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCAGGCATCTCTGGCTAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATG                                                                                    >  SRR4302543.3712003/1‑94 (MQ=60)

            GTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGC                                                                                   >  SRR4302543.1433019/1‑94 (MQ=60)

            GTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGC                                                                                   <  SRR4302543.1747441/94‑1 (MQ=60)

             TCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCG                                                                                  >  SRR4302543.7599725/1‑94 (MQ=60)

             TCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCG                                                                                  >  SRR4302543.6281763/1‑94 (MQ=60)

               ATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGA                                                                                >  SRR4302543.3367691/1‑94 (MQ=60)

                TTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGAT                                                                               >  SRR4302543.2542240/1‑94 (MQ=60)

                TTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGAT                                                                               <  SRR4302543.4514463/94‑1 (MQ=60)

                TTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGAT                                                                               <  SRR4302543.7695488/94‑1 (MQ=60)

                CTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGAT                                                                               >  SRR4302543.3267979/1‑94 (MQ=60)

                 TATCTGACGCTTATCACCTGAGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATA                                                                              >  SRR4302543.131529/1‑94 (MQ=60)

                  ATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAA                                                                             >  SRR4302543.920617/1‑94 (MQ=60)

                    CTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACT                                                                           >  SRR4302543.7977519/1‑94 (MQ=60)

                    CTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACT                                                                           >  SRR4302543.6645327/1‑94 (MQ=60)

                    CTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACT                                                                           >  SRR4302543.3028554/1‑94 (MQ=60)

                    CTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATACGCGGATAACT                                                                           <  SRR4302543.7870824/94‑1 (MQ=60)

                      GACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCG                                                                         <  SRR4302543.4969018/94‑1 (MQ=60)

                      GACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCG                                                                         <  SRR4302543.6211514/94‑1 (MQ=60)

                        CGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGC                                                                       <  SRR4302543.1847851/94‑1 (MQ=60)

                        CGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGC                                                                       <  SRR4302543.1749230/94‑1 (MQ=60)

                         GCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCG                                                                      <  SRR4302543.5010821/94‑1 (MQ=60)

                         GCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCG                                                                      >  SRR4302543.1305422/1‑94 (MQ=60)

                          CTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGC                                                                     >  SRR4302543.3595122/1‑94 (MQ=60)

                            TATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGG                                                                   >  SRR4302543.2059371/1‑94 (MQ=60)

                               CACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAA                                                                >  SRR4302543.5458/1‑94 (MQ=60)

                                ACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAG                                                               <  SRR4302543.3396842/94‑1 (MQ=60)

                                ACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAG                                                               <  SRR4302543.4792832/94‑1 (MQ=60)

                                ACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAG                                                               <  SRR4302543.4392797/94‑1 (MQ=60)

                                ACCTGTGGTGTGCGTCTGGCATCTATGGCTAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGAGAACTCGGCGCGAGAAG                                                               <  SRR4302543.4392798/94‑1 (MQ=60)

                                 cctgcggtggtcttcgcgcagCTCTGGCCAATACGGCAATGCCACAGTTAGTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGT                                                              <  SRR4302543.6458607/73‑1 (MQ=60)

                                 CCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGT                                                              <  SRR4302543.6458608/94‑1 (MQ=60)

                                 CCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGG                                                              >  SRR4302543.1258246/1‑94 (MQ=60)

                                  CTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTG                                                             >  SRR4302543.2260456/1‑94 (MQ=60)

                                   TGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGG                                                            <  SRR4302543.1785723/94‑1 (MQ=60)

                                   TGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGG                                                            >  SRR4302543.5211466/1‑94 (MQ=60)

                                   TGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGG                                                            <  SRR4302543.3765303/94‑1 (MQ=60)

                                   TGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGG                                                            <  SRR4302543.5565316/94‑1 (MQ=60)

                                    GTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGT                                                           >  SRR4302543.893888/1‑94 (MQ=60)

                                    GTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGT                                                           >  SRR4302543.6503500/1‑94 (MQ=60)

                                    GTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGT                                                           <  SRR4302543.2778284/94‑1 (MQ=60)

                                     TGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTG                                                          <  SRR4302543.7875849/94‑1 (MQ=60)

                                      GGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGA                                                         <  SRR4302543.3494291/94‑1 (MQ=60)

                                      GGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGA                                                         >  SRR4302543.1865344/1‑94 (MQ=60)

                                       GTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGAT                                                        >  SRR4302543.1688671/1‑94 (MQ=60)

                                       GTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGAT                                                        >  SRR4302543.7119485/1‑94 (MQ=60)

                                       GTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGAT                                                        <  SRR4302543.2059548/94‑1 (MQ=60)

                                       GTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGAT                                                        >  SRR4302543.5148763/1‑94 (MQ=60)

                                         GTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGT                                                      >  SRR4302543.3953958/1‑94 (MQ=60)

                                         GTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGT                                                      >  SRR4302543.6687359/1‑94 (MQ=60)

                                         GTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGT                                                      >  SRR4302543.6368199/1‑94 (MQ=60)

                                         GTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGT                                                      <  SRR4302543.7043407/94‑1 (MQ=60)

                                          TGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTT                                                     >  SRR4302543.5793649/1‑94 (MQ=60)

                                           GCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTT                                                    >  SRR4302543.108143/1‑94 (MQ=60)

                                            CTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTA                                                   >  SRR4302543.472664/1‑94 (MQ=60)

                                             TTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAG                                                  <  SRR4302543.3477246/94‑1 (MQ=60)

                                             TTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAG                                                  <  SRR4302543.2862270/94‑1 (MQ=60)

                                             TTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAG                                                  <  SRR4302543.6571048/94‑1 (MQ=60)

                                             TTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAG                                                  >  SRR4302543.4001889/1‑94 (MQ=60)

                                             TTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAG                                                  >  SRR4302543.6845851/1‑94 (MQ=60)

                                              TCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGC                                                 <  SRR4302543.3507733/94‑1 (MQ=60)

                                                TGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTC                                               <  SRR4302543.3958920/94‑1 (MQ=60)

                                                 GGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCG                                              <  SRR4302543.5456666/94‑1 (MQ=60)

                                                 GGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCG                                              <  SRR4302543.2886768/94‑1 (MQ=60)

                                                   CATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGT                                            <  SRR4302543.2780391/94‑1 (MQ=60)

                                                    ATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTG                                           >  SRR4302543.2885354/1‑94 (MQ=60)

                                                    ATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTG                                           >  SRR4302543.7518544/1‑94 (MQ=60)

                                                    ATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTG                                           >  SRR4302543.3685350/1‑94 (MQ=60)

                                                      TTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAT                                         <  SRR4302543.6806353/94‑1 (MQ=60)

                                                      CTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAT                                         <  SRR4302543.1069900/94‑1 (MQ=60)

                                                      CTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGAT                                         >  SRR4302543.5611471/1‑94 (MQ=60)

                                                       TCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATG                                        <  SRR4302543.1850884/94‑1 (MQ=60)

                                                          GGCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGT                                     >  SRR4302543.5112436/1‑94 (MQ=60)

                                                           GCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCTGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTG                                    <  SRR4302543.2798074/94‑1 (MQ=60)

                                                           GCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTG                                    <  SRR4302543.1930389/94‑1 (MQ=60)

                                                           GCCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTG                                    <  SRR4302543.2798073/94‑1 (MQ=60)

                                                            CCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGC                                   >  SRR4302543.3266884/1‑94 (MQ=60)

                                                            CCAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGC                                   >  SRR4302543.7798025/1‑94 (MQ=60)

                                                             CAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCG                                  >  SRR4302543.3135630/1‑94 (MQ=60)

                                                             CAATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCG                                  <  SRR4302543.3308604/94‑1 (MQ=60)

                                                              AATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGC                                 >  SRR4302543.4306397/1‑94 (MQ=60)

                                                              AATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGC                                 <  SRR4302543.5679173/94‑1 (MQ=60)

                                                               ATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCA                                >  SRR4302543.2717075/1‑94 (MQ=60)

                                                               ATACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCA                                >  SRR4302543.3126420/1‑94 (MQ=60)

                                                                TACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAG                               <  SRR4302543.1569605/94‑1 (MQ=60)

                                                                TACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAG                               >  SRR4302543.6763809/1‑94 (MQ=60)

                                                                TACGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAG                               <  SRR4302543.4901491/94‑1 (MQ=60)

                                                                  CGGCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCT                             >  SRR4302543.4250752/1‑94 (MQ=60)

                                                                  CGGCAATGCCACAGTTATTTGCTCTGGCACGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCT                             <  SRR4302543.7055608/94‑1 (MQ=60)

                                                                    GCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTT                           <  SRR4302543.7873453/94‑1 (MQ=60)

                                                                    GCAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTT                           <  SRR4302543.9909/94‑1 (MQ=60)

                                                                     CAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTC                          >  SRR4302543.4503950/1‑94 (MQ=60)

                                                                     CAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTC                          >  SRR4302543.3575467/1‑94 (MQ=60)

                                                                     CAATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTC                          <  SRR4302543.4398796/94‑1 (MQ=60)

                                                                      AATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCT                         >  SRR4302543.2868933/1‑94 (MQ=60)

                                                                      AATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGGTTAGCTCGGTGATGCGTGCGCAGCTTTCT                         >  SRR4302543.7927003/1‑94 (MQ=60)

                                                                         GCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTG                      >  SRR4302543.645835/1‑94 (MQ=60)

                                                                         GCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTG                      <  SRR4302543.7606518/94‑1 (MQ=60)

                                                                         GCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTG                      >  SRR4302543.5522333/1‑94 (MQ=60)

                                                                          CCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGG                     >  SRR4302543.7552188/1‑94 (MQ=60)

                                                                          CCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGG                     <  SRR4302543.40735/94‑1 (MQ=60)

                                                                          CCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGG                     >  SRR4302543.3471203/1‑94 (MQ=60)

                                                                           CACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGC                    >  SRR4302543.2675216/1‑94 (MQ=60)

                                                                           CACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGC                    >  SRR4302543.7294513/1‑94 (MQ=60)

                                                                             CAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCAT                  <  SRR4302543.1799294/94‑1 (MQ=60)

                                                                               GTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGG                <  SRR4302543.3862584/94‑1 (MQ=60)

                                                                               GTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGGGGTGTTTAGCTCGGTGATGCGTGAGCAGCTTTCTCTGGCGTGG                >  SRR4302543.1380948/1‑94 (MQ=60)

                                                                                TTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGG               >  SRR4302543.3806484/1‑94 (MQ=60)

                                                                                TTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGG               >  SRR4302543.2028672/1‑94 (MQ=60)

                                                                                TTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGG               <  SRR4302543.6319884/94‑1 (MQ=60)

                                                                                  ATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGGAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTT             >  SRR4302543.7874607/1‑94 (MQ=60)

                                                                                  ATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTT             >  SRR4302543.1275799/1‑94 (MQ=60)

                                                                                  ATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTT             <  SRR4302543.7572898/94‑1 (MQ=60)

                                                                                  ATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTT             >  SRR4302543.5063332/1‑94 (MQ=60)

                                                                                  ATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGCGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTT             >  SRR4302543.4849788/1‑94 (MQ=60)

                                                                                   TTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTA            <  SRR4302543.1209796/94‑1 (MQ=60)

                                                                                    TTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTAT           <  SRR4302543.6815359/94‑1 (MQ=60)

                                                                                    TTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTAT           <  SRR4302543.7681347/94‑1 (MQ=60)

                                                                                     TGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATC          <  SRR4302543.2126762/94‑1 (MQ=60)

                                                                                     TGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATC          >  SRR4302543.1700901/1‑94 (MQ=60)

                                                                                     TGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATC          >  SRR4302543.557885/1‑94 (MQ=60)

                                                                                       CTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGG        >  SRR4302543.1434043/1‑94 (MQ=60)

                                                                                       CTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGG        >  SRR4302543.2954934/1‑94 (MQ=60)

                                                                                       CTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGG        <  SRR4302543.3983910/94‑1 (MQ=60)

                                                                                       CTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGG        <  SRR4302543.4651511/94‑1 (MQ=60)

                                                                                       CTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGCTATCGG        >  SRR4302543.2955155/1‑94 (MQ=60)

                                                                                        TCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGT       <  SRR4302543.2343210/94‑1 (MQ=60)

                                                                                         CTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTC      >  SRR4302543.1999350/1‑94 (MQ=60)

                                                                                            GCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCAC   <  SRR4302543.869287/94‑1 (MQ=60)

                                                                                            GCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCAC   <  SRR4302543.1170225/94‑1 (MQ=60)

                                                                                             CGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACC  >  SRR4302543.979220/1‑94 (MQ=60)

TTGCATTTAATCGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCAATGCCACAGTTATTTGCTCTGGAGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTGATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACC  >  NC_000913/77910‑78096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`