Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,845,852	T→C	T61A (ACC→GCC)	selD ←	selenophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,845,852	0	T	C	99.5%	586.4 / NA	190	T61A (ACC→GCC)	selD	selenophosphate synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (95/94); minor base A (1/0); total (96/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT  >  NC_000913/1845763‑1845941

 cTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCGTAAAGAAGTCGGTGGTACTGATAACGCTGGAGCCa                                                                                       >  1:1274891/1‑93 (MQ=255)

 cTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCa                                                                                       <  2:5958735/93‑1 (MQ=255)

 cTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCa                                                                                       >  1:2303813/1‑93 (MQ=255)

  tGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAt                                                                                      <  2:5072460/93‑1 (MQ=255)

   gATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCAtt                                                                                     >  1:5709067/1‑93 (MQ=255)

    aTGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTg                                                                                    <  1:5990538/93‑1 (MQ=255)

       gCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGccc                                                                                 >  2:6697906/1‑93 (MQ=255)

        ggTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCa                                                                                <  2:3808760/92‑1 (MQ=255)

        cGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCa                                                                                <  1:113513/93‑1 (MQ=255)

          ttAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCCTTGGCCCGa                                                                              >  2:3215717/1‑93 (MQ=255)

          ttAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGa                                                                              >  1:2068634/1‑93 (MQ=255)

           tAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAt                                                                             <  2:7101124/93‑1 (MQ=255)

           tAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAt                                                                             <  2:4545143/93‑1 (MQ=255)

            aGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATc                                                                            >  2:7732133/1‑93 (MQ=255)

             gTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCg                                                                           <  1:5866968/93‑1 (MQ=255)

              tCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGt                                                                          >  2:5161319/1‑93 (MQ=255)

                gccgcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTac                                                                        >  1:3449271/1‑93 (MQ=255)

                  cgcAATGCGGCCAAAATCGGAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTacac                                                                      >  1:3988428/1‑93 (MQ=255)

                   gcAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAcc                                                                     >  1:3297923/1‑93 (MQ=255)

                    cAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccg                                                                    >  2:2645870/1‑93 (MQ=255)

                    cAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccg                                                                    >  1:5060534/1‑93 (MQ=255)

                     caTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgc                                                                   <  1:44361/92‑1 (MQ=255)

                     caTGCCGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgc                                                                   <  1:44358/92‑1 (MQ=255)

                     aaTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgc                                                                   <  2:685210/93‑1 (MQ=255)

                     aaTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgc                                                                   >  1:3968444/1‑93 (MQ=255)

                      aTGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgcc                                                                  >  2:1589507/1‑93 (MQ=255)

                       tGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAccgccg                                                                 >  2:802981/1‑93 (MQ=255)

                        gCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACcgccgc                                                                >  1:3921622/1‑93 (MQ=255)

                          ggCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAt                                                              >  1:2443359/1‑93 (MQ=255)

                           gCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtc                                                             >  1:2986020/1‑93 (MQ=255)

                           gCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtc                                                             <  1:10813/93‑1 (MQ=255)

                           gCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtc                                                             <  1:6899588/93‑1 (MQ=255)

                              aaaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtc                                                          >  2:7355216/1‑93 (MQ=255)

                               aaaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAtcgtcg                                                         <  2:1323198/93‑1 (MQ=255)

                                aaTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTcgc                                                        <  1:1666746/93‑1 (MQ=255)

                                 aTCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTcgcg                                                       >  2:1199985/1‑93 (MQ=255)

                                  tCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTTGTACTGATAACGCTGGCGCCATTGCGCAGATCGTACACCGCCGCATCGTCGCgg                                                      <  2:4834423/93‑1 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    >  1:6134868/1‑93 (MQ=255)

                                    gAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGt                                                    <  1:4241082/93‑1 (MQ=255)

                                     aaaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGtt                                                   >  2:2578080/1‑93 (MQ=255)

                                     aaaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGtt                                                   <  2:7134746/93‑1 (MQ=255)

                                      aaGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGttt                                                  <  2:2814293/93‑1 (MQ=255)

                                       aGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTc                                                 <  1:1709829/93‑1 (MQ=255)

                                        ggcttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGACAACGCTGGCGCCATTGCCCAGATTGTACACCGCCGCATCGTCGCGGGTTTCa                                                <  2:5271143/90‑1 (MQ=255)

                                        ggATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCa                                                >  2:1326259/1‑93 (MQ=255)

                                         gATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAt                                               >  1:5952360/1‑93 (MQ=255)

                                           ttATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTa                                             <  1:5506391/93‑1 (MQ=255)

                                            tATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAc                                            <  2:145106/93‑1 (MQ=255)

                                              tCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAccc                                          >  2:6226832/1‑93 (MQ=255)

                                              tCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAccc                                          <  2:2942533/93‑1 (MQ=255)

                                               cAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCca                                         <  1:4842100/93‑1 (MQ=255)

                                               cAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCca                                         >  2:2793258/1‑93 (MQ=255)

                                                aaCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCcac                                        >  2:1041586/1‑93 (MQ=255)

                                                aaCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCcac                                        >  1:3753885/1‑93 (MQ=255)

                                                 aCGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCcaca                                       >  1:927992/1‑93 (MQ=255)

                                                  cGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACaa                                      <  2:3988427/93‑1 (MQ=255)

                                                   gATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAg                                     >  2:3030637/1‑93 (MQ=255)

                                                     tCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCa                                   >  1:780268/1‑93 (MQ=255)

                                                      cGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCaa                                  <  1:57487/93‑1 (MQ=255)

                                                       ggCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCaaa                                 <  2:7889072/93‑1 (MQ=255)

                                                        gCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAt                                <  2:248248/93‑1 (MQ=255)

                                                        gCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAt                                >  1:6526241/1‑93 (MQ=255)

                                                         cATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAtt                               <  2:7291041/93‑1 (MQ=255)

                                                         cATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAtt                               <  1:2636312/93‑1 (MQ=255)

                                                          aTAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTc                              <  1:1530448/93‑1 (MQ=255)

                                                           tAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCg                             >  2:1149456/1‑93 (MQ=255)

                                                           tAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCg                             >  2:2355558/1‑93 (MQ=255)

                                                            aaaGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCgg                            >  1:1353715/1‑93 (MQ=255)

                                                            aaaGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCgg                            >  2:1808130/1‑93 (MQ=255)

                                                            aaaGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCgg                            >  1:6315782/1‑93 (MQ=255)

                                                             aagaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGa                           >  1:1618576/1‑93 (MQ=255)

                                                             aagaagTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGa                           <  2:6623438/93‑1 (MQ=255)

                                                                 agTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaa                       <  2:6404000/93‑1 (MQ=255)

                                                                  gTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaac                      <  2:2172837/93‑1 (MQ=255)

                                                                  gTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaac                      <  2:3653596/93‑1 (MQ=255)

                                                                   tCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATcaaca                     <  2:2598893/93‑1 (MQ=255)

                                                                     ggtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACaaa                   <  1:5415467/93‑1 (MQ=255)

                                                                     ggtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTCTCATTACCCACAAGCAAATTCGGATCAACaaa                   <  2:6586408/93‑1 (MQ=255)

                                                                      gtggtACTGATAACTCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAc                  <  2:2037550/93‑1 (MQ=255)

                                                                      gtggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAc                  <  2:873767/93‑1 (MQ=255)

                                                                        ggtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACtt                >  2:5469786/1‑93 (MQ=255)

                                                                         gtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTc               >  2:7477962/1‑93 (MQ=255)

                                                                         gtACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTc               <  1:520827/93‑1 (MQ=255)

                                                                            cTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGcc            <  2:196934/93‑1 (MQ=255)

                                                                             tGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCt           >  2:6768719/1‑93 (MQ=255)

                                                                              gATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTg          >  1:1898488/1‑93 (MQ=255)

                                                                              gATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGGTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTg          >  2:6923048/1‑93 (MQ=255)

                                                                               aTAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGc         <  2:7587495/93‑1 (MQ=255)

                                                                               aTAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGc         <  1:7437348/93‑1 (MQ=255)

                                                                               aTAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGc         <  1:7670968/93‑1 (MQ=255)

                                                                                 aaCGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTc       <  1:5774812/93‑1 (MQ=255)

                                                                                 aaCGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTc       <  2:5885525/93‑1 (MQ=255)

                                                                                   cGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCAc     <  2:725129/93‑1 (MQ=255)

                                                                                   cGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCAc     >  2:3731167/1‑93 (MQ=255)

                                                                                   cGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCAc     <  1:6844982/93‑1 (MQ=255)

                                                                                     cTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTa   <  1:1699206/93‑1 (MQ=255)

                                                                                     cTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTa   <  2:2851018/93‑1 (MQ=255)

                                                                                     cTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTa   >  1:7207974/1‑93 (MQ=255)

                                                                                      tGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAATTTCGCCTGCTCACTAt  >  1:7034281/1‑93 (MQ=255)

                                                                                      tGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAt  >  1:2796738/1‑93 (MQ=255)

GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT  >  NC_000913/1845763‑1845941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  NC_000913/1845759‑1845945

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGC                                                                                               <  SRR4302543.6061339/94‑1 (MQ=60)

 ATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCG                                                                                              >  SRR4302543.5979096/1‑94 (MQ=60)

 ATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCG                                                                                              >  SRR4302543.5979095/1‑94 (MQ=60)

   CGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCC                                                                                            >  SRR4302543.5897128/1‑94 (MQ=60)

    GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCGTAAAGAAGTCGGTGGTACTGATAACGCTGGAGCCA                                                                                           >  SRR4302543.1286269/1‑94 (MQ=60)

    GCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCA                                                                                           >  SRR4302543.2324799/1‑94 (MQ=60)

      TGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATT                                                                                         >  SRR4302543.5761152/1‑94 (MQ=60)

        ATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGC                                                                                       <  SRR4302543.6045439/94‑1 (MQ=60)

        ATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGC                                                                                       <  SRR4302543.3993999/94‑1 (MQ=60)

            CGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAG                                                                                   <  SRR4302543.114547/94‑1 (MQ=60)

             GTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGA                                                                                  <  SRR4302543.5337621/94‑1 (MQ=60)

             GTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGA                                                                                  >  SRR4302543.2087124/1‑94 (MQ=60)

              TTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGAT                                                                                 <  SRR4302543.7621136/94‑1 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               <  SRR4302543.949841/94‑1 (MQ=60)

                AGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCG                                                                               <  SRR4302543.7216616/94‑1 (MQ=60)

                 GTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGT                                                                              <  SRR4302543.5920374/94‑1 (MQ=60)

                   CGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTAC                                                                            >  SRR4302543.3480162/1‑94 (MQ=60)

                    GCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACA                                                                           >  SRR4302543.5225183/1‑94 (MQ=60)

                     CCGCAATGCGGCCAAAATCGGAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACAC                                                                          >  SRR4302543.4023867/1‑94 (MQ=60)

                      CGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACC                                                                         >  SRR4302543.1706702/1‑94 (MQ=60)

                      CGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACC                                                                         >  SRR4302543.6761263/1‑94 (MQ=60)

                      CGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACC                                                                         >  SRR4302543.3327679/1‑94 (MQ=60)

                       GCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCG                                                                        >  SRR4302543.5106514/1‑94 (MQ=60)

                        CAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGC                                                                       >  SRR4302543.4003621/1‑94 (MQ=60)

                         CATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      <  SRR4302543.44766/94‑1 (MQ=60)

                         CATGCCGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      <  SRR4302543.44763/94‑1 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      >  SRR4302543.5538543/1‑94 (MQ=60)

                         AATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCC                                                                      <  SRR4302543.44765/94‑1 (MQ=60)

                          ATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCG                                                                     >  SRR4302543.2256962/1‑94 (MQ=60)

                          ATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCG                                                                     >  SRR4302543.1562213/1‑94 (MQ=60)

                           TGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGC                                                                    >  SRR4302543.3956231/1‑94 (MQ=60)

                             CGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCAT                                                                  >  SRR4302543.2465389/1‑94 (MQ=60)

                              GGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATC                                                                 >  SRR4302543.3013136/1‑94 (MQ=60)

                               GCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCG                                                                <  SRR4302543.6964427/94‑1 (MQ=60)

                               GCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCG                                                                <  SRR4302543.10906/94‑1 (MQ=60)

                                 CAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTC                                                              <  SRR4302543.3145787/94‑1 (MQ=60)

                                  AAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCG                                                             >  SRR4302543.5621027/1‑94 (MQ=60)

                                  AAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCG                                                             >  SRR4302543.867654/1‑94 (MQ=60)

                                    AATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCG                                                           <  SRR4302543.1681671/94‑1 (MQ=60)

                                    AAGCGAAAGAATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCG                                                           <  SRR4302543.6275040/94‑1 (MQ=60)

                                       CGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGT                                                        >  SRR4302543.6192979/1‑94 (MQ=60)

                                       CGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGT                                                        >  SRR4302543.5805817/1‑94 (MQ=60)

                                        GAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTT                                                       <  SRR4302543.4279562/94‑1 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302543.6082050/1‑94 (MQ=60)

                                         AAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTT                                                      >  SRR4302543.2740777/1‑94 (MQ=60)

                                           AGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCA                                                    <  SRR4302543.1725125/94‑1 (MQ=60)

                                            GGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGTTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAT                                                   >  SRR4302543.6006836/1‑94 (MQ=60)

                                            GGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCAT                                                   >  SRR4302543.6006835/1‑94 (MQ=60)

                                               TTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTAC                                                <  SRR4302543.5556387/94‑1 (MQ=60)

                                                TATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACC                                               <  SRR4302543.1826966/94‑1 (MQ=60)

                                                 ATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCC                                              <  SRR4302543.5767774/94‑1 (MQ=60)

                                                   CAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            >  SRR4302543.3786911/1‑94 (MQ=60)

                                                   CAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCAC                                            <  SRR4302543.4886250/94‑1 (MQ=60)

                                                    AACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACA                                           >  SRR4302543.936106/1‑94 (MQ=60)

                                                    AACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACA                                           <  SRR4302543.1721490/94‑1 (MQ=60)

                                                      CGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAG                                         <  SRR4302543.135816/94‑1 (MQ=60)

                                                       GATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGC                                        >  SRR4302543.2560483/1‑94 (MQ=60)

                                                        ATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCA                                       <  SRR4302543.4569368/94‑1 (MQ=60)

                                                        ATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCA                                       <  SRR4302543.7307013/94‑1 (MQ=60)

                                                        ATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCA                                       >  SRR4302543.787123/1‑94 (MQ=60)

                                                          CGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAA                                     <  SRR4302543.57990/94‑1 (MQ=60)

                                                           GGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAT                                    >  SRR4302543.7003500/1‑94 (MQ=60)

                                                           GGCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAAT                                    >  SRR4302543.6587694/1‑94 (MQ=60)

                                                            GCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATT                                   >  SRR4302543.1167974/1‑94 (MQ=60)

                                                            GCATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATT                                   <  SRR4302543.4381754/94‑1 (MQ=60)

                                                             CATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTC                                  <  SRR4302543.2660186/94‑1 (MQ=60)

                                                             CATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTC                                  <  SRR4302543.3722400/94‑1 (MQ=60)

                                                              ATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCG                                 >  SRR4302543.382230/1‑94 (MQ=60)

                                                              ATAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCG                                 <  SRR4302543.1544169/94‑1 (MQ=60)

                                                               TAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGG                                >  SRR4302543.1365822/1‑94 (MQ=60)

                                                               TAAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGG                                >  SRR4302543.6375181/1‑94 (MQ=60)

                                                                AAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGA                               >  SRR4302543.1633001/1‑94 (MQ=60)

                                                                AAAGAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGA                               >  SRR4302543.4005641/1‑94 (MQ=60)

                                                                   GAAGTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCA                            <  SRR4302543.7801906/94‑1 (MQ=60)

                                                                      GTCGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACA                         >  SRR4302543.1474027/1‑94 (MQ=60)

                                                                        CGGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAA                       >  SRR4302543.6912535/1‑94 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      <  SRR4302543.5464734/94‑1 (MQ=60)

                                                                         GGTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAAC                      <  SRR4302543.2064107/94‑1 (MQ=60)

                                                                          GTGGTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACT                     <  SRR4302543.6477711/94‑1 (MQ=60)

                                                                             GTACTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCG                  <  SRR4302543.525660/94‑1 (MQ=60)

                                                                                CTGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCT               >  SRR4302543.163908/1‑94 (MQ=60)

                                                                                 TGATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTG              >  SRR4302543.1915567/1‑94 (MQ=60)

                                                                                   ATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCT            <  SRR4302543.7743656/94‑1 (MQ=60)

                                                                                   ATAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCT            <  SRR4302543.7508641/94‑1 (MQ=60)

                                                                                    TAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCCCAAGCAAATTCGGATCAACAAACTTCGCCTGCTC           >  SRR4302543.5043589/1‑94 (MQ=60)

                                                                                    TAACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTC           >  SRR4302543.4068655/1‑94 (MQ=60)

                                                                                     AACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCA          <  SRR4302543.5827378/94‑1 (MQ=60)

                                                                                     AACGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCA          <  SRR4302543.2454748/94‑1 (MQ=60)

                                                                                       CGCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACT        <  SRR4302543.6909573/94‑1 (MQ=60)

                                                                                        GCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTA       >  SRR4302543.2971689/1‑94 (MQ=60)

                                                                                        GCTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTA       >  SRR4302543.7277853/1‑94 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCACATTCGCATCAACAAACTTCGCCTGCTCcttag      >  SRR4302543.2822297/1‑89 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAATTTCGCCTGCTCACTAT      >  SRR4302543.7101977/1‑94 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      >  SRR4302543.2822298/1‑94 (MQ=60)

                                                                                         CTGGCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTAT      <  SRR4302543.1714436/94‑1 (MQ=60)

                                                                                            GCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCA   <  SRR4302543.6416187/94‑1 (MQ=60)

                                                                                            GCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCA   <  SRR4302543.7509776/94‑1 (MQ=60)

                                                                                            GCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCA   <  SRR4302543.3056585/94‑1 (MQ=60)

                                                                                            GCGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCA   <  SRR4302543.5198628/94‑1 (MQ=60)

                                                                                             CGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  <  SRR4302543.4927306/94‑1 (MQ=60)

TATCGCTGATGGCGTTAGTCGCCGCAATGCGGCCAAAATCGAAAGGATTATCAACGATCGGCATAAAGAAGTCGGTGGTACTGATAACGCTGGTGCCATTGCCCAGATCGTACACCGCCGCATCGTCGCGGGTTTCATTACCCACAAGCAAATTCGGATCAACAAACTTCGCCTGCTCACTATGCAG  >  NC_000913/1845759‑1845945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`