Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,958,588 T→C M168T (ATG→ACG)  argS → arginine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,958,5880TC99.1% 658.4 / ‑3.0 214M168T (ATG→ACG) argSarginine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (105/107);  total (106/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01

Only 100 of 222 total aligned reads displayed.

TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAA  >  NC_000913/1958498‑1958675
                                                                                          |                                                                                       
ttGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGc                                                                                       <  2:3434263/93‑1 (MQ=255)
   gTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGa                                                                                    <  2:6297856/93‑1 (MQ=255)
    tGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAt                                                                                   <  1:7086970/93‑1 (MQ=255)
     gACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAtt                                                                                  <  2:4787864/93‑1 (MQ=255)
        gcagcaGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCa                                                                               <  2:2411654/93‑1 (MQ=255)
        gcagcaGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCa                                                                               <  2:1992227/93‑1 (MQ=255)
         cagcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAt                                                                              <  2:1134118/93‑1 (MQ=255)
          agcatTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATg                                                                             <  2:7138953/93‑1 (MQ=255)
           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                            >  2:5930896/1‑93 (MQ=255)
           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                            >  1:2341876/1‑93 (MQ=255)
           gcagTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATgg                                                                            <  2:727214/93‑1 (MQ=255)
             agTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCt                                                                          >  2:363403/1‑93 (MQ=255)
             agTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCt                                                                          >  1:1297551/1‑93 (MQ=255)
              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                         >  2:6857673/1‑93 (MQ=255)
              gTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTg                                                                         >  2:7738329/1‑93 (MQ=255)
                gCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGa                                                                       >  1:3871774/1‑93 (MQ=255)
                  gTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaa                                                                     >  2:3833398/1‑93 (MQ=255)
                   tACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGaaaa                                                                    <  2:4868637/93‑1 (MQ=255)
                    aCTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAag                                                                   <  1:2775542/93‑1 (MQ=255)
                      tctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagca                                                                 >  2:134018/1‑93 (MQ=255)
                      tctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagca                                                                 <  1:1541354/93‑1 (MQ=255)
                       ctGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcag                                                                <  2:733001/93‑1 (MQ=255)
                          gAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAagcagcag                                                             >  2:4808615/1‑93 (MQ=255)
                            gTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                           >  1:1895396/1‑93 (MQ=255)
                            gTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                           >  1:5625532/1‑93 (MQ=255)
                            gTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAgcagcagca                                                           <  2:5500282/93‑1 (MQ=255)
                             ttCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGcagcagcag                                                          <  2:3895939/93‑1 (MQ=255)
                             ttCCTCGCTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGcagcagcag                                                          <  2:2205481/93‑1 (MQ=255)
                               ccTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGa                                                        <  2:2609510/93‑1 (MQ=255)
                               ccTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGa                                                        <  1:3812397/93‑1 (MQ=255)
                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                       >  1:1126661/1‑93 (MQ=255)
                                cTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaa                                                       >  2:7191063/1‑93 (MQ=255)
                                 tCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGaaa                                                      >  1:1469964/1‑93 (MQ=255)
                                   ggTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAc                                                    >  1:7220848/1‑93 (MQ=255)
                                      cacaAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGcc                                                 <  2:4652102/93‑1 (MQ=255)
                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                               <  2:4065396/93‑1 (MQ=255)
                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                               <  1:3703894/93‑1 (MQ=255)
                                        caAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCgg                                               >  1:7608414/1‑93 (MQ=255)
                                         aaaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGt                                              <  1:6859613/93‑1 (MQ=255)
                                          aaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTg                                             >  1:3542422/1‑93 (MQ=255)
                                          aaGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTg                                             >  1:7546499/1‑93 (MQ=255)
                                            gTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaa                                           <  2:1298580/93‑1 (MQ=255)
                                            gTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaa                                           >  1:3279150/1‑93 (MQ=255)
                                             tGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaaa                                          >  2:3140375/1‑93 (MQ=255)
                                             tGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGaaa                                          >  1:677536/1‑93 (MQ=255)
                                              gATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAAt                                         <  2:6284308/93‑1 (MQ=255)
                                               gttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATg                                        >  1:3362981/2‑93 (MQ=255)
                                               aTTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATg                                        <  2:2460603/93‑1 (MQ=255)
                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       >  1:3218043/1‑93 (MQ=255)
                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       <  2:4440726/93‑1 (MQ=255)
                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       >  2:4559980/1‑93 (MQ=255)
                                                ttCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATgg                                       <  2:1532398/93‑1 (MQ=255)
                                                 tCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGa                                      <  1:5466027/93‑1 (MQ=255)
                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    >  2:2063176/1‑93 (MQ=255)
                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    <  1:757471/93‑1 (MQ=255)
                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    <  1:5285374/93‑1 (MQ=255)
                                                   gcgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgc                                    <  1:6064197/93‑1 (MQ=255)
                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                   <  1:3018561/93‑1 (MQ=255)
                                                    cgcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgct                                   <  2:1379959/93‑1 (MQ=255)
                                                     gcAAACCACGTCGGCGACTGGGGCCCTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctg                                  >  2:7588573/1‑93 (MQ=255)
                                                     gcAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctg                                  >  1:6505671/1‑93 (MQ=255)
                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                >  2:7922015/1‑93 (MQ=255)
                                                       aaaCCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAgctggc                                >  2:658509/1‑93 (MQ=255)
                                                           cACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAc                            <  2:4604906/93‑1 (MQ=255)
                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                           <  2:5395305/93‑1 (MQ=255)
                                                            aCGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGAcc                           <  1:4467937/93‑1 (MQ=255)
                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                          <  1:7263920/93‑1 (MQ=255)
                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                          <  1:20396/93‑1 (MQ=255)
                                                             cGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCt                          <  1:4850116/93‑1 (MQ=255)
                                                              ctCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCtt                         <  1:789526/92‑1 (MQ=255)
                                                              ctCGGCGACTGGGGCACGCATTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCtt                         <  2:289550/92‑1 (MQ=255)
                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                        >  1:877859/1‑93 (MQ=255)
                                                               tCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGCGAAATGGAGCTGGCTGACCTTg                        >  1:4639409/1‑93 (MQ=255)
                                                               tCGGCGACTGGAGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTg                        <  1:57461/93‑1 (MQ=255)
                                                                cGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGa                       >  2:5559668/1‑93 (MQ=255)
                                                                 ggCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGaa                      <  2:6451376/93‑1 (MQ=255)
                                                                   cGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAgg                    <  2:6038581/93‑1 (MQ=255)
                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                   <  1:815736/93‑1 (MQ=255)
                                                                    gACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGt                   >  1:7057627/1‑93 (MQ=255)
                                                                     aCTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGtt                  >  2:521029/1‑93 (MQ=255)
                                                                      cTGGGGCACTCAGTTCGGTACGGTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGttt                 >  1:7526506/1‑93 (MQ=255)
                                                                      cTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGttt                 >  2:3793238/1‑93 (MQ=255)
                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAGGCAGCAGCAGGAAAACGCCGGTGCAATGGAGCTGGCTGACCTTGAAGGTTTc                >  1:1269390/1‑93 (MQ=255)
                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                <  2:2754326/93‑1 (MQ=255)
                                                                       tGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTc                >  2:7644846/1‑93 (MQ=255)
                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCt               >  1:4072551/1‑93 (MQ=255)
                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCt               <  1:106554/93‑1 (MQ=255)
                                                                        ggggCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGGGAAATGGAGCGGGCTGGCCTTGAAGTGTTCt               >  1:2534360/1‑93 (MQ=255)
                                                                             aCTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcgc          >  2:2368718/1‑93 (MQ=255)
                                                                              cTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACcgcg         >  1:6411948/1‑93 (MQ=255)
                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  1:4781008/1‑93 (MQ=255)
                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  2:5406246/1‑93 (MQ=255)
                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  2:3503219/1‑93 (MQ=255)
                                                                               tCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGa        >  1:3579478/1‑93 (MQ=255)
                                                                                 aGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATg      >  1:7385522/1‑93 (MQ=255)
                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    >  2:1742522/1‑93 (MQ=255)
                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    <  1:1448267/93‑1 (MQ=255)
                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    >  1:1277076/1‑93 (MQ=255)
                                                                                   ttCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCg    >  1:4240930/1‑93 (MQ=255)
                                                                                     cGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGaa  <  2:5625565/93‑1 (MQ=255)
                                                                                          |                                                                                       
TTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAA  >  NC_000913/1958498‑1958675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  >  NC_000913/1958495‑1958681
                                                                                             |                                                                                             
TTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTAC                                                                                               <  SRR4302543.6926732/94‑1 (MQ=60)
  ATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGC                                                                                             <  SRR4302543.2387029/94‑1 (MQ=60)
      GTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGAT                                                                                         <  SRR4302543.7982480/94‑1 (MQ=60)
       TGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATT                                                                                        <  SRR4302543.7155794/94‑1 (MQ=60)
       TGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATT                                                                                        <  SRR4302543.6389075/94‑1 (MQ=60)
        GACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTG                                                                                       <  SRR4302543.2105212/94‑1 (MQ=60)
        GACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTG                                                                                       <  SRR4302543.2934748/94‑1 (MQ=60)
         ACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGC                                                                                      <  SRR4302543.1984965/94‑1 (MQ=60)
           GCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCAT                                                                                    <  SRR4302543.6909305/94‑1 (MQ=60)
             AGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGG                                                                                  >  SRR4302543.2363120/1‑94 (MQ=60)
             AGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGG                                                                                  >  SRR4302543.793543/1‑94 (MQ=60)
               CAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCT                                                                                <  SRR4302543.5209980/94‑1 (MQ=60)
               CAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCT                                                                                >  SRR4302543.1309086/1‑94 (MQ=60)
                 GTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGG                                                                              <  SRR4302543.4109816/94‑1 (MQ=60)
                 GTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGG                                                                              >  SRR4302543.3639563/1‑94 (MQ=60)
                  TGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGA                                                                             >  SRR4302543.3905960/1‑94 (MQ=60)
                    CGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAA                                                                           >  SRR4302543.5740434/1‑94 (MQ=60)
                     GTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAA                                                                          >  SRR4302543.5932613/1‑94 (MQ=60)
                     GTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAA                                                                          >  SRR4302543.6817505/1‑94 (MQ=60)
                     GTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAA                                                                          >  SRR4302543.2063220/1‑94 (MQ=60)
                      TACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAG                                                                         <  SRR4302543.6100896/94‑1 (MQ=60)
                       ACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGC                                                                        >  SRR4302543.4850549/1‑94 (MQ=60)
                       ACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGC                                                                        <  SRR4302543.2800814/94‑1 (MQ=60)
                         TCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAG                                                                      <  SRR4302543.1555157/94‑1 (MQ=60)
                          CTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGC                                                                     >  SRR4302543.162814/1‑94 (MQ=60)
                          CTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGC                                                                     <  SRR4302543.1467456/94‑1 (MQ=60)
                           TGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCA                                                                    <  SRR4302543.4440291/94‑1 (MQ=60)
                            GGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAG                                                                   <  SRR4302543.3238636/94‑1 (MQ=60)
                            GGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAG                                                                   <  SRR4302543.3850734/94‑1 (MQ=60)
                              AGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCA                                                                 >  SRR4302543.3248645/1‑94 (MQ=60)
                              AGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCA                                                                 >  SRR4302543.1912441/1‑94 (MQ=60)
                              AGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCA                                                                 >  SRR4302543.5676795/1‑94 (MQ=60)
                              AGTTACTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCA                                                                 >  SRR4302543.4545602/1‑94 (MQ=60)
                                 TCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGA                                                              >  SRR4302543.7240023/1‑94 (MQ=60)
                                  CCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAA                                                             >  SRR4302543.6547179/1‑94 (MQ=60)
                                  CCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAA                                                             <  SRR4302543.3846004/94‑1 (MQ=60)
                                  CCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAA                                                             >  SRR4302543.1136883/1‑94 (MQ=60)
                                  CCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAA                                                             >  SRR4302543.7620765/1‑94 (MQ=60)
                                   CTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAA                                                            >  SRR4302543.1483203/1‑94 (MQ=60)
                                    TCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAA                                                           >  SRR4302543.4077155/1‑94 (MQ=60)
                                     CGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAC                                                          >  SRR4302543.7290833/1‑94 (MQ=60)
                                     CGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAC                                                          <  SRR4302543.1186791/94‑1 (MQ=60)
                                         CACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCG                                                      <  SRR4302543.7484083/94‑1 (MQ=60)
                                          ACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGG                                                     >  SRR4302543.7680649/1‑94 (MQ=60)
                                           CAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGT                                                    <  SRR4302543.3736456/94‑1 (MQ=60)
                                            AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   <  SRR4302543.6924271/94‑1 (MQ=60)
                                            AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   <  SRR4302543.6924270/94‑1 (MQ=60)
                                            AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   <  SRR4302543.907469/94‑1 (MQ=60)
                                            AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   >  SRR4302543.3573898/1‑94 (MQ=60)
                                            AAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTG                                                   >  SRR4302543.7618396/1‑94 (MQ=60)
                                             AAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGA                                                  >  SRR4302543.3596890/1‑94 (MQ=60)
                                              AGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAA                                                 >  SRR4302543.3308755/1‑94 (MQ=60)
                                              AGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAA                                                 >  SRR4302543.2600417/1‑94 (MQ=60)
                                               GTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAA                                                >  SRR4302543.683771/1‑94 (MQ=60)
                                                 GGTTCGCGCAAACCACTTCTGCGACGGGGGCCCTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATG                                              <  SRR4302543.2388995/94‑1 (MQ=60)
                                                 GGTTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATG                                              >  SRR4302543.3393220/1‑94 (MQ=60)
                                                  ATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGG                                             >  SRR4302543.7405554/1‑94 (MQ=60)
                                                  ATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGG                                             >  SRR4302543.1286225/1‑94 (MQ=60)
                                                  ATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGG                                             <  SRR4302543.5630604/94‑1 (MQ=60)
                                                  ATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGG                                             >  SRR4302543.4266032/1‑94 (MQ=60)
                                                  ATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGG                                             >  SRR4302543.3247121/1‑94 (MQ=60)
                                                    TCGCGCAAACCCCGTCGGCGACTGGGGCACTCAGTTCGCTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAG                                           <  SRR4302543.850662/94‑1 (MQ=60)
                                                    TCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAG                                           <  SRR4302543.5515556/94‑1 (MQ=60)
                                                      GCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCT                                         >  SRR4302543.6993628/1‑94 (MQ=60)
                                                      GCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCT                                         <  SRR4302543.764163/94‑1 (MQ=60)
                                                      GCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCT                                         <  SRR4302543.5333076/94‑1 (MQ=60)
                                                      GCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCT                                         <  SRR4302543.6121665/94‑1 (MQ=60)
                                                       CGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTG                                        <  SRR4302543.3045858/94‑1 (MQ=60)
                                                       CGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTG                                        >  SRR4302543.6566957/1‑94 (MQ=60)
                                                        GCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGG                                       >  SRR4302543.3494516/1‑94 (MQ=60)
                                                         CAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGC                                      <  SRR4302543.3056140/94‑1 (MQ=60)
                                                         CAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGC                                      >  SRR4302543.780041/1‑94 (MQ=60)
                                                          AAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCT                                     >  SRR4302543.7295475/1‑94 (MQ=60)
                                                          AAACCACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCT                                     >  SRR4302543.1302777/1‑94 (MQ=60)
                                                               ACGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCT                                <  SRR4302543.4508446/94‑1 (MQ=60)
                                                                CGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTT                               <  SRR4302543.5460041/94‑1 (MQ=60)
                                                                CGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTT                               <  SRR4302543.7334106/94‑1 (MQ=60)
                                                                CGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTT                               <  SRR4302543.1550618/94‑1 (MQ=60)
                                                                CGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTT                               <  SRR4302543.20585/94‑1 (MQ=60)
                                                                CGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTT                               <  SRR4302543.4767586/94‑1 (MQ=60)
                                                                CGTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTT                               <  SRR4302543.4894344/94‑1 (MQ=60)
                                                                 GTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTG                              >  SRR4302543.885656/1‑94 (MQ=60)
                                                                 GTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGCGAAATGGAGCTGGCTGACCTTG                              >  SRR4302543.4681601/1‑94 (MQ=60)
                                                                 CTCGGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTG                              <  SRR4302543.796470/94‑1 (MQ=60)
                                                                  TCGGCGACTGGAGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGA                             <  SRR4302543.57964/94‑1 (MQ=60)
                                                                    GGCGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAG                           >  SRR4302543.1121459/1‑94 (MQ=60)
                                                                      CGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGT                         >  SRR4302543.7126117/1‑94 (MQ=60)
                                                                      CGACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGT                         >  SRR4302543.7126118/1‑94 (MQ=60)
                                                                       GACTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTT                        <  SRR4302543.822917/94‑1 (MQ=60)
                                                                        ACTGGGGCACTCAGTTCGGTACGGTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTT                       >  SRR4302543.7598312/1‑94 (MQ=60)
                                                                         CTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAGGCAGCAGCAGGAAAACGCCGGTGCAATGGAGCTGGCTGACCTTGAAGGTTTC                      >  SRR4302543.1280737/1‑94 (MQ=60)
                                                                         CTGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTC                      >  SRR4302543.7674457/1‑94 (MQ=60)
                                                                          TGTGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCT                     <  SRR4302543.3333070/94‑1 (MQ=60)
                                                                          TGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCT                     >  SRR4302543.4109035/1‑94 (MQ=60)
                                                                          TGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCT                     <  SRR4302543.1460441/94‑1 (MQ=60)
                                                                          TGGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGGGAAATGGAGCGGGCTGGCCTTGAAGTGTTCT                     >  SRR4302543.2557444/1‑94 (MQ=60)
                                                                           GGGGCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTA                    <  SRR4302543.107534/94‑1 (MQ=60)
                                                                              GCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCG                 <  SRR4302543.3804080/94‑1 (MQ=60)
                                                                              GCACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCG                 <  SRR4302543.3628204/94‑1 (MQ=60)
                                                                               CACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGC                <  SRR4302543.7604/94‑1 (MQ=60)
                                                                                ACTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCG               >  SRR4302543.6472327/1‑94 (MQ=60)
                                                                                 CTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGA              >  SRR4302543.7402706/1‑94 (MQ=60)
                                                                                 CTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGA              >  SRR4302543.4824704/1‑94 (MQ=60)
                                                                                 CTCAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGA              >  SRR4302543.3611099/1‑94 (MQ=60)
                                                                                   CAGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATG            >  SRR4302543.7456491/1‑94 (MQ=60)
                                                                                    AGTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGC           >  SRR4302543.5120845/1‑94 (MQ=60)
                                                                                     GTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCG          >  SRR4302543.4279409/1‑94 (MQ=60)
                                                                                     GTTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCG          >  SRR4302543.1288469/1‑94 (MQ=60)
                                                                                      TTCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGA         <  SRR4302543.1461353/94‑1 (MQ=60)
                                                                                       TCGGTACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAA        >  SRR4302543.1339416/1‑94 (MQ=60)
                                                                                           TACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGCCCTTGAAGGTTTCTACCGCGATGCGAAAAAG    <  SRR4302543.6744009/94‑1 (MQ=60)
                                                                                           TACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAG    <  SRR4302543.3709844/94‑1 (MQ=60)
                                                                                            ACGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGC   >  SRR4302543.6180558/1‑94 (MQ=60)
                                                                                             CGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCTGGTGAAATGGGGCTGGCTGACCTTGAAGGCTTCTACCGCGAgcggaaaaagct  >  SRR4302543.2547962/1‑82 (MQ=60)
                                                                                             CGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  <  SRR4302543.2096563/94‑1 (MQ=60)
                                                                                             CGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  >  SRR4302543.7943852/1‑94 (MQ=60)
                                                                                             CGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  <  SRR4302543.3217064/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTATTGGTGACGCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGCGACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAACGCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCA  >  NC_000913/1958495‑1958681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑