Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,973,518	A→T	D254E (GAT→GAA)	motB ←	MotB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,973,518	0	A	T	99.5%	778.8 / ‑3.7	219	D254E (GAT→GAA)	motB	MotB
Reads supporting (aligned to +/- strand): ref base A (0/1); new base T (116/102); total (116/103)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

Only 100 of 239 total aligned reads displayed.

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATC  >  NC_000913/1973428‑1973605

 ggCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttca                                                                                      <  1:5935161/93‑1 (MQ=255)

 ggCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGGTGACGGcttca                                                                                      >  2:1073483/1‑93 (MQ=255)

  gCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGGTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcttcat                                                                                     >  2:701717/1‑93 (MQ=255)

   cTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCttcatc                                                                                    <  2:3469018/93‑1 (MQ=255)

    tCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTtcatca                                                                                   <  2:7658501/93‑1 (MQ=255)

    tCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTtcatca                                                                                   >  1:2884573/1‑93 (MQ=255)

    tCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTtcatca                                                                                   >  2:2127957/1‑93 (MQ=255)

    tCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTtcatca                                                                                   <  2:1572465/93‑1 (MQ=255)

     cATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAg                                                                                  <  1:4464038/93‑1 (MQ=255)

     cATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAg                                                                                  >  2:2869621/1‑93 (MQ=255)

      aTTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAgg                                                                                 >  1:3552973/1‑93 (MQ=255)

      aTTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAgg                                                                                 >  1:1761983/1‑93 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCTTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGGCGGTTGACGGGTTCATCAggg                                                                                >  1:1924598/1‑92 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAggc                                                                                >  1:6067096/1‑92 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                <  2:6293385/93‑1 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                >  1:488104/1‑93 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                >  2:7793270/1‑93 (MQ=255)

       ttCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGt                                                                                >  1:1056729/1‑93 (MQ=255)

        tCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGGTGACGGCTTCATCAGGTc                                                                               >  1:384180/1‑93 (MQ=255)

          tGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcg                                                                             >  2:7493619/1‑93 (MQ=255)

          tGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcg                                                                             <  2:7107366/93‑1 (MQ=255)

           ggCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcgc                                                                            >  1:1108188/1‑93 (MQ=255)

           ggCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCcgc                                                                            >  1:7295111/1‑93 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAt                                                                         <  2:2126017/93‑1 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAt                                                                         <  2:1478736/93‑1 (MQ=255)

              tttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAt                                                                         <  1:165436/93‑1 (MQ=255)

               ttCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATc                                                                        <  1:5741662/93‑1 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                     >  1:1199696/1‑93 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                     >  1:6441317/1‑93 (MQ=255)

                  ggCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCt                                                                     >  2:3340220/1‑93 (MQ=255)

                   gCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCtt                                                                    <  2:3894061/93‑1 (MQ=255)

                    cGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTa                                                                   <  2:4790030/93‑1 (MQ=255)

                    cGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTa                                                                   <  1:7385398/93‑1 (MQ=255)

                    cGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTa                                                                   >  1:6393307/1‑93 (MQ=255)

                     gTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTaa                                                                  <  2:6679631/93‑1 (MQ=255)

                     gTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTaa                                                                  <  1:1342972/93‑1 (MQ=255)

                      ttttCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAg                                                                 >  2:369592/1‑93 (MQ=255)

                       tttCATGCCCAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGGTGCGACGGGTAACGGCTTGACCAAGTCCTCGATCACCTAAgg                                                                >  2:5368413/1‑92 (MQ=255)

                          cATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCa                                                             <  2:7217447/93‑1 (MQ=255)

                           aggCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                            <  2:6864311/91‑1 (MQ=255)

                           aTGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                            >  1:4973521/1‑93 (MQ=255)

                           aTGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAt                                                            <  2:5111826/93‑1 (MQ=255)

                            tGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATc                                                           <  1:2407318/93‑1 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                         <  2:2350656/93‑1 (MQ=255)

                              cAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGt                                                         >  2:2710926/1‑93 (MQ=255)

                               aaaaTGGCCGGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCCACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGtt                                                        <  2:6893817/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                      <  1:6849015/93‑1 (MQ=255)

                                 aaTGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgc                                                      <  1:96215/93‑1 (MQ=255)

                                   tGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgccg                                                    <  1:2605078/93‑1 (MQ=255)

                                   tGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTgccg                                                    >  2:1676686/1‑93 (MQ=255)

                                      ccTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCa                                                 <  2:3920978/93‑1 (MQ=255)

                                       cTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAt                                                >  2:7710281/1‑93 (MQ=255)

                                        tGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATg                                               >  2:2975847/1‑93 (MQ=255)

                                        tGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATg                                               >  2:6564447/1‑93 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGc                                              <  1:5184972/93‑1 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGc                                              <  2:695221/93‑1 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGc                                              >  2:1924564/1‑93 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGc                                              <  1:3949418/93‑1 (MQ=255)

                                         gTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGGTGCCGCCATGc                                              >  1:6172251/1‑93 (MQ=255)

                                            cGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcga                                           <  2:2174876/93‑1 (MQ=255)

                                              gCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCcgacg                                         >  1:2442544/1‑93 (MQ=255)

                                                 tgtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGaca                                      <  1:3882285/93‑1 (MQ=255)

                                                 tgtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGaca                                      <  2:12089/93‑1 (MQ=255)

                                                 tgtttgttAAGTACCAGCAGGCTGATGCGACGGTTGACCGCTTCATCCAGCCCGCGATCGCTTAAGCGCATCGTTACCGCCATGCCgccgacc                                      >  2:4148055/1‑92 (MQ=255)

                                                  gtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGacac                                     >  1:5128596/1‑93 (MQ=255)

                                                  gtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGacac                                     <  2:3419060/93‑1 (MQ=255)

                                                  gtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGacac                                     <  2:126549/93‑1 (MQ=255)

                                                  gtttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGacac                                     >  2:6181428/1‑93 (MQ=255)

                                                    ttgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACga                                   >  1:2750117/1‑92 (MQ=255)

                                                     tgttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTa                                  >  1:5864328/1‑93 (MQ=255)

                                                      gttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTaa                                 <  1:1413042/93‑1 (MQ=255)

                                                       ttCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTCac                                >  1:7769538/1‑93 (MQ=255)

                                                        tCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAaca                               >  2:3084984/1‑93 (MQ=255)

                                                           gTACCAGCAGGCTGATGCGACGGTTGACGGATTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAAGACACGTAACACtt                            >  1:5415893/1‑93 (MQ=255)

                                                            tACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACttt                           <  1:266413/93‑1 (MQ=255)

                                                              ccAGCAGGCGGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGc                         <  2:2437402/93‑1 (MQ=255)

                                                               cagcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGcc                        >  2:5840139/1‑93 (MQ=255)

                                                               cagcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGcc                        <  1:7092069/93‑1 (MQ=255)

                                                                agcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGccc                       >  2:6027838/1‑92 (MQ=255)

                                                                agcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGAACGCTTAAGCGCCACGTTGCCGCCATGCCGACGACACGTAACACTTTGCCg                       >  2:6534265/1‑93 (MQ=255)

                                                                 gcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                      <  1:5538937/93‑1 (MQ=255)

                                                                 gcagGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGc                      >  1:7834465/1‑93 (MQ=255)

                                                                   agGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTa                    <  2:6335850/93‑1 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAt                   >  1:7561103/1‑93 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAt                   <  2:1862241/93‑1 (MQ=255)

                                                                    ggCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGAAACGGAACACTTTGCCGCTAt                   >  2:1306697/1‑93 (MQ=255)

                                                                     gCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATc                  <  1:4261901/93‑1 (MQ=255)

                                                                      cTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                 <  2:2933534/93‑1 (MQ=255)

                                                                      cTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATcc                 >  2:15929/1‑93 (MQ=255)

                                                                       tGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCa                <  1:3275977/93‑1 (MQ=255)

                                                                          tGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAcc             >  2:4582707/1‑93 (MQ=255)

                                                                          tGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAAcc             <  1:6509755/93‑1 (MQ=255)

                                                                             gACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTc          <  2:1785706/93‑1 (MQ=255)

                                                                              aCGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTcc         <  1:3475095/93‑1 (MQ=255)

                                                                                 gTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCCACCCTCCGAc      >  2:1391785/1‑93 (MQ=255)

                                                                                 gTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAc      >  1:4243532/1‑93 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc     >  1:666230/1‑93 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc     >  1:7697001/1‑93 (MQ=255)

                                                                                  ttGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAcc     >  2:3302486/1‑93 (MQ=255)

                                                                                     aCGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATc  >  1:1029389/1‑93 (MQ=255)

TGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATC  >  NC_000913/1973428‑1973605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  NC_000913/1973427‑1973611

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             >  SRR4302543.5794840/1‑94 (MQ=60)

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             <  SRR4302543.6633445/94‑1 (MQ=60)

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             >  SRR4302543.4150768/1‑94 (MQ=60)

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             >  SRR4302543.4826615/1‑94 (MQ=60)

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTC                                                                                             >  SRR4302543.7432910/1‑94 (MQ=60)

  GGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCAT                                                                                           <  SRR4302543.5989446/94‑1 (MQ=60)

    CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         >  SRR4302543.2910824/1‑94 (MQ=60)

    CTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCA                                                                                         >  SRR4302543.2761037/1‑94 (MQ=60)

     TCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAG                                                                                        <  SRR4302543.2985193/94‑1 (MQ=60)

      CATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGG                                                                                       <  SRR4302543.4504491/94‑1 (MQ=60)

      CATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGG                                                                                       >  SRR4302543.3584479/1‑94 (MQ=60)

      CATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGG                                                                                       >  SRR4302543.1777733/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCTTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGGCGGTTGACGGGTTCATCAGGG                                                                                      >  SRR4302543.1941942/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      <  SRR4302543.6468259/94‑1 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302543.5480898/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302543.492615/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGT                                                                                      >  SRR4302543.1066213/1‑94 (MQ=60)

       ATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGC                                                                                      >  SRR4302543.6124581/1‑94 (MQ=60)

        TTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGGTGACGGCTTCATCAGGTC                                                                                     >  SRR4302543.387730/1‑94 (MQ=60)

         TCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCC                                                                                    <  SRR4302543.2789289/94‑1 (MQ=60)

         TCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCC                                                                                    <  SRR4302543.3955551/94‑1 (MQ=60)

         TCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCC                                                                                    <  SRR4302543.5089036/94‑1 (MQ=60)

          CTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCG                                                                                   >  SRR4302543.7418850/1‑94 (MQ=60)

          CTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCG                                                                                   <  SRR4302543.1082224/94‑1 (MQ=60)

           TGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGC                                                                                  >  SRR4302543.7365503/1‑94 (MQ=60)

           TGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGC                                                                                  >  SRR4302543.1118191/1‑94 (MQ=60)

             GCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGA                                                                                >  SRR4302543.6871834/1‑94 (MQ=60)

               TTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATC                                                                              <  SRR4302543.166862/94‑1 (MQ=60)

                TTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCG                                                                             <  SRR4302543.5794010/94‑1 (MQ=60)

                  CGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCT                                                                           >  SRR4302543.6502001/1‑94 (MQ=60)

                  CGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCT                                                                           >  SRR4302543.1210498/1‑94 (MQ=60)

                    GCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTA                                                                         >  SRR4302543.6453492/1‑94 (MQ=60)

                     CGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAA                                                                        <  SRR4302543.7456365/94‑1 (MQ=60)

                     CGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAA                                                                        <  SRR4302543.7689556/94‑1 (MQ=60)

                      GTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAG                                                                       <  SRR4302543.1354967/94‑1 (MQ=60)

                      GTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGGTGACGGCTTCATCAGGTCCGCGATCGCTTAAG                                                                       >  SRR4302543.1780736/1‑94 (MQ=60)

                       TTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGC                                                                      <  SRR4302543.7664885/94‑1 (MQ=60)

                       TTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGC                                                                      <  SRR4302543.5606848/94‑1 (MQ=60)

                         TTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGC                                                                    >  SRR4302543.5261974/1‑94 (MQ=60)

                           CATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAT                                                                  >  SRR4302543.6957603/1‑94 (MQ=60)

                           CATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCAT                                                                  >  SRR4302543.5018793/1‑94 (MQ=60)

                             TGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCG                                                                <  SRR4302543.2429076/94‑1 (MQ=60)

                               CAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTT                                                              >  SRR4302543.1398680/1‑94 (MQ=60)

                                AAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTG                                                             >  SRR4302543.85444/1‑94 (MQ=60)

                                  AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCC                                                           <  SRR4302543.97113/94‑1 (MQ=60)

                                  AATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCC                                                           <  SRR4302543.6913627/94‑1 (MQ=60)

                                   ATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCG                                                          <  SRR4302543.606131/94‑1 (MQ=60)

                                   ATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCG                                                          <  SRR4302543.4468897/94‑1 (MQ=60)

                                    TGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGC                                                         <  SRR4302543.2628738/94‑1 (MQ=60)

                                    TGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGC                                                         >  SRR4302543.255579/1‑94 (MQ=60)

                                     GGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCC                                                        >  SRR4302543.750244/1‑94 (MQ=60)

                                       CCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACTGCTTCATCAGGACCCGGATAGCTTAAGCGCATCGTTGCCGCCAT                                                      >  SRR4302543.1833351/1‑94 (MQ=60)

                                       CCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCAT                                                      <  SRR4302543.6143666/94‑1 (MQ=60)

                                         TGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGGTGCCGCCATGC                                                    >  SRR4302543.6230628/1‑94 (MQ=60)

                                          GTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCC                                                   <  SRR4302543.3984349/94‑1 (MQ=60)

                                          GTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCC                                                   <  SRR4302543.5231752/94‑1 (MQ=60)

                                          GTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCC                                                   <  SRR4302543.4933947/94‑1 (MQ=60)

                                             CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302543.6795799/94‑1 (MQ=60)

                                             CGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAC                                                <  SRR4302543.6482849/94‑1 (MQ=60)

                                              GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               <  SRR4302543.2687536/94‑1 (MQ=60)

                                              GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               >  SRR4302543.5092560/1‑94 (MQ=60)

                                              GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               <  SRR4302543.3808541/94‑1 (MQ=60)

                                              GGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACG                                               >  SRR4302543.2464565/1‑94 (MQ=60)

                                               GCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGA                                              >  SRR4302543.949797/1‑94 (MQ=60)

                                                  TGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACAC                                           <  SRR4302543.3916563/94‑1 (MQ=60)

                                                  TGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACAC                                           >  SRR4302543.5174951/1‑94 (MQ=60)

                                                    TTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGA                                         >  SRR4302543.2775131/1‑94 (MQ=60)

                                                     TTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTA                                        >  SRR4302543.5917710/1‑94 (MQ=60)

                                                       GTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATTGTTGCCGCCATGCCGACGACACGTAAC                                      >  SRR4302543.363657/1‑94 (MQ=60)

                                                       GTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTCAC                                      >  SRR4302543.7842838/1‑94 (MQ=60)

                                                       GTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAAC                                      <  SRR4302543.1425821/94‑1 (MQ=60)

                                                       GTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAAC                                      >  SRR4302543.7842837/1‑94 (MQ=60)

                                                         TCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACAC                                    <  SRR4302543.5862625/94‑1 (MQ=60)

                                                         TCAGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACAC                                    >  SRR4302543.3903149/1‑94 (MQ=60)

                                                           AGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTT                                  >  SRR4302543.1275512/1‑94 (MQ=60)

                                                           AGTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTT                                  >  SRR4302543.352961/1‑94 (MQ=60)

                                                           AGTACCAGCAGGCTGATGCGACGGTTGACGGATTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGAAGACACGTAACACTT                                  >  SRR4302543.5465164/1‑94 (MQ=60)

                                                            GTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTT                                 >  SRR4302543.5177660/1‑94 (MQ=60)

                                                            GTACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTT                                 <  SRR4302543.7209038/94‑1 (MQ=60)

                                                             TACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTG                                <  SRR4302543.268754/94‑1 (MQ=60)

                                                             TACCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTG                                <  SRR4302543.5953824/94‑1 (MQ=60)

                                                               CCAGGAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCC                              <  SRR4302543.5529646/94‑1 (MQ=60)

                                                               CCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCC                              >  SRR4302543.6711778/1‑94 (MQ=60)

                                                               CCAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGCCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCC                              >  SRR4302543.2299556/1‑94 (MQ=60)

                                                                CAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCG                             <  SRR4302543.7160950/94‑1 (MQ=60)

                                                                CAGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCG                             >  SRR4302543.4653241/1‑94 (MQ=60)

                                                                 AGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGC                            >  SRR4302543.7908219/1‑94 (MQ=60)

                                                                 AGCAGGCTGATGCGACGGTTGACGGCTTCATCAGGCCCGGGATCGTTTAAGCGCATGGTTGCCccactgcggccgccaggaaaacttttgcgct                            >  SRR4302543.6145415/1‑63 (MQ=43)

                                                                  GCAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCT                           <  SRR4302543.5589301/94‑1 (MQ=60)

                                                                   CAGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTA                          >  SRR4302543.428626/1‑94 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         >  SRR4302543.7633076/1‑94 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         <  SRR4302543.193548/94‑1 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         >  SRR4302543.6175691/1‑94 (MQ=60)

                                                                    AGGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTAT                         <  SRR4302543.4012632/94‑1 (MQ=60)

                                                                     GGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATC                        >  SRR4302543.2688883/1‑94 (MQ=60)

                                                                     GGCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATC                        <  SRR4302543.118119/94‑1 (MQ=60)

                                                                      GCTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCC                       <  SRR4302543.4300610/94‑1 (MQ=60)

                                                                       CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGCCACGTACCACTTTGCCGCTATCCA                      >  SRR4302543.1700705/1‑94 (MQ=60)

                                                                       CTGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCA                      <  SRR4302543.2614/94‑1 (MQ=60)

                                                                        TGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAC                     >  SRR4302543.1054178/1‑94 (MQ=60)

                                                                        TGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAA                     <  SRR4302543.3305563/94‑1 (MQ=60)

                                                                        TGATGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAA                     <  SRR4302543.3305564/94‑1 (MQ=60)

                                                                           TGCGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCC                  <  SRR4302543.6571077/94‑1 (MQ=60)

                                                                             CGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTC                >  SRR4302543.6354412/1‑94 (MQ=60)

                                                                             CGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTC                >  SRR4302543.1597444/1‑94 (MQ=60)

                                                                             CGACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTC                >  SRR4302543.1432268/1‑94 (MQ=60)

                                                                              GACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCC               >  SRR4302543.1157096/1‑94 (MQ=60)

                                                                               ACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAATCCTCCG              >  SRR4302543.24586/1‑94 (MQ=60)

                                                                               ACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCG              <  SRR4302543.3506222/94‑1 (MQ=60)

                                                                               ACGGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCG              <  SRR4302543.3478328/94‑1 (MQ=60)

                                                                                 GGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAC            >  SRR4302543.4282040/1‑94 (MQ=60)

                                                                                 GGTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGAC            >  SRR4302543.6569917/1‑94 (MQ=60)

                                                                                  GTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACC           >  SRR4302543.7769856/1‑94 (MQ=60)

                                                                                  GTTGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACC           >  SRR4302543.672360/1‑94 (MQ=60)

                                                                                    TGACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCAT         >  SRR4302543.7883670/1‑94 (MQ=60)

                                                                                     GACGGCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATC        >  SRR4302543.1038514/1‑94 (MQ=60)

                                                                                         GCTTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTT    <  SRR4302543.6622400/94‑1 (MQ=60)

                                                                                           TTCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  SRR4302543.4189023/1‑94 (MQ=60)

CTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTCCGCGATCGCTTAAGCGCATCGTTGCCGCCATGCCGACGACACGTAACACTTTGCCGCTATCCAACCCTCCGACCATCAGTTCG  >  NC_000913/1973427‑1973611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`