Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,975,051 G→A A38V (GCC→GTC)  motA ← MotA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,975,0510GA99.6% 832.3 / ‑5.5 233A38V (GCC→GTC) motAMotA
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (119/113);  total (120/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.26e-01

Only 100 of 238 total aligned reads displayed.

GAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACC  >  NC_000913/1974961‑1975140
                                                                                          |                                                                                         
gAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACa                                                                                         >  1:555595/1‑93 (MQ=255)
 aGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCCTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaa                                                                                        >  2:109907/1‑93 (MQ=255)
    gacgaAACAGCAACGGCAGCTCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaataa                                                                                     <  2:1764473/93‑1 (MQ=255)
    gacgaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaataa                                                                                     <  1:4246393/93‑1 (MQ=255)
    gacgaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaataa                                                                                     >  1:4898006/1‑93 (MQ=255)
     acgaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACaataat                                                                                    >  2:6686727/1‑93 (MQ=255)
       gaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCa                                                                                  >  2:2738515/1‑93 (MQ=255)
       gaAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCa                                                                                  >  1:7857759/1‑93 (MQ=255)
        aaaCAGCAGCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCAc                                                                                 <  2:5577242/93‑1 (MQ=255)
        aaaCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCAc                                                                                 >  2:3371680/1‑93 (MQ=255)
        aaaCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCAc                                                                                 <  1:7083065/93‑1 (MQ=255)
          aCAGCAGCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCa                                                                               <  2:4550299/93‑1 (MQ=255)
          aCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCa                                                                               >  2:2231379/1‑93 (MQ=255)
          aCAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCa                                                                               <  1:4649642/93‑1 (MQ=255)
             gCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGtt                                                                            <  2:6271313/93‑1 (MQ=255)
             gCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGtt                                                                            <  1:6713923/93‑1 (MQ=255)
              cAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTc                                                                           <  2:228708/93‑1 (MQ=255)
               aaCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCa                                                                          <  1:1424786/93‑1 (MQ=255)
                aCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAg                                                                         >  1:1964597/1‑93 (MQ=255)
                aCGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAg                                                                         >  2:154335/1‑93 (MQ=255)
                  ggCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCg                                                                       >  1:2177031/1‑93 (MQ=255)
                   gCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCgg                                                                      >  1:5282432/1‑93 (MQ=255)
                     aGCGCCTTCAGCGTGCCTTTAATCGCTTTTCCATTATTGCCGACGATCAACGACCCAATCCTTGCACCGCCAATAATCACCAGTTCAGCGCGt                                                                    >  1:1012992/1‑93 (MQ=255)
                     aGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGt                                                                    <  1:179617/93‑1 (MQ=255)
                       cgcCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTg                                                                  <  1:6950480/93‑1 (MQ=255)
                        gcCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGa                                                                 >  2:10141/1‑93 (MQ=255)
                         ccTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAt                                                                <  2:296127/93‑1 (MQ=255)
                         ccTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAt                                                                >  1:5166133/1‑93 (MQ=255)
                          cTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATa                                                               <  2:1397853/93‑1 (MQ=255)
                           ttCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATag                                                              >  1:3114807/1‑93 (MQ=255)
                           ttCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATag                                                              >  1:541146/1‑93 (MQ=255)
                            tCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATaga                                                             <  1:5594695/93‑1 (MQ=255)
                            tCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATaga                                                             <  2:534995/93‑1 (MQ=255)
                            tCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGCTCAGCGGGTTGATaga                                                             >  1:1292237/1‑93 (MQ=255)
                              aGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGt                                                           >  1:6809456/1‑93 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTg                                                          >  2:4528277/1‑93 (MQ=255)
                               gCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTAATAGAGTg                                                          <  1:1548732/93‑1 (MQ=255)
                                cGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGGTGATAGAGAGc                                                         >  1:6369138/1‑93 (MQ=255)
                                 gTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCt                                                        <  2:6735671/93‑1 (MQ=255)
                                  tGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTc                                                       >  1:3694927/1‑93 (MQ=255)
                                   gCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTcc                                                      >  2:4568818/1‑93 (MQ=255)
                                   gCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTcc                                                      >  2:4197953/1‑93 (MQ=255)
                                   gCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTcc                                                      >  2:5150638/1‑93 (MQ=255)
                                     cTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCaa                                                    <  2:7878088/93‑1 (MQ=255)
                                     cTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCaa                                                    <  2:3731746/93‑1 (MQ=255)
                                      tttAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCAACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAg                                                   >  2:7234022/1‑93 (MQ=255)
                                       ttAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAgg                                                  <  1:1853469/93‑1 (MQ=255)
                                        tAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGc                                                 <  2:7194076/93‑1 (MQ=255)
                                        tAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGc                                                 <  2:6794564/93‑1 (MQ=255)
                                        tAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGc                                                 <  2:6075065/93‑1 (MQ=255)
                                         aaTCTCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCt                                                <  2:5904928/93‑1 (MQ=255)
                                         aaTCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCt                                                <  2:1948108/93‑1 (MQ=255)
                                           tCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTc                                              >  2:5617006/1‑93 (MQ=255)
                                           tCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTc                                              >  2:1376685/1‑93 (MQ=255)
                                            cGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTcc                                             <  2:7472641/93‑1 (MQ=255)
                                            cGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTcc                                             >  1:2414075/1‑93 (MQ=255)
                                             gCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCa                                            >  1:275988/1‑93 (MQ=255)
                                              cTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCAc                                           <  1:5652663/93‑1 (MQ=255)
                                               tttGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCAcc                                          <  1:6581221/93‑1 (MQ=255)
                                                  gCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGt                                       >  1:5307024/1‑93 (MQ=255)
                                                    cATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtca                                     >  1:5922554/1‑93 (MQ=255)
                                                    cATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtca                                     <  2:4715462/93‑1 (MQ=255)
                                                      ttattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatc                                   >  1:4784819/1‑93 (MQ=255)
                                                      ttattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatc                                   <  2:5531463/93‑1 (MQ=255)
                                                      ttattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatc                                   <  1:7018866/93‑1 (MQ=255)
                                                      ttagtGCCGACCATAAACGGCCCAAGCCCTTCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatc                                   <  2:5906869/93‑1 (MQ=255)
                                                       tattGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGtcatca                                  >  2:378631/1‑93 (MQ=255)
                                                         ttGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCaaa                                >  2:1323688/1‑93 (MQ=255)
                                                         ttGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCaaa                                >  1:6285384/1‑93 (MQ=255)
                                                           gCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATa                              >  1:4604264/1‑93 (MQ=255)
                                                             cgacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCCCCAAGGCTTCCACCGGTCATCAAATAAc                            <  2:4176040/93‑1 (MQ=255)
                                                              gacgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAcc                           <  2:1760422/93‑1 (MQ=255)
                                                               acgaTAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAccg                          >  1:7728925/1‑93 (MQ=255)
                                                                     aaCGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGaaa                    >  1:7304526/1‑93 (MQ=255)
                                                                     aaCGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGaaa                    >  1:4112412/1‑93 (MQ=255)
                                                                     aaCGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGaaa                    <  2:5653362/93‑1 (MQ=255)
                                                                     aaCGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGaaa                    <  1:3337510/93‑1 (MQ=255)
                                                                       cGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAAc                  <  2:7696985/93‑1 (MQ=255)
                                                                         aCCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTg                >  1:5131025/1‑93 (MQ=255)
                                                                          cccAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTga               >  1:5754359/1‑92 (MQ=255)
                                                                          cccAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGt               <  2:3105589/93‑1 (MQ=255)
                                                                          cccAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGt               <  1:3569280/93‑1 (MQ=255)
                                                                          cccAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGt               >  2:2094677/1‑93 (MQ=255)
                                                                           ccAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTa              <  1:6055477/93‑1 (MQ=255)
                                                                            cAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             >  1:2632427/1‑93 (MQ=255)
                                                                            cAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAc             >  2:2547494/1‑93 (MQ=255)
                                                                            cAATCCCTACACCGACAATTATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGAAc             <  2:7025584/93‑1 (MQ=255)
                                                                             aaTCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAcc            <  2:7535306/93‑1 (MQ=255)
                                                                             aaTCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAcc            >  1:6251658/1‑93 (MQ=255)
                                                                              aTCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCg           >  1:4748672/1‑93 (MQ=255)
                                                                               tCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCga          >  2:2047441/1‑93 (MQ=255)
                                                                                 ccTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCgaga        >  1:2864807/1‑93 (MQ=255)
                                                                                  cTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaa       >  2:450668/1‑93 (MQ=255)
                                                                                  cTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaa       >  2:3528186/1‑93 (MQ=255)
                                                                                   tGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaac      >  2:68629/1‑93 (MQ=255)
                                                                                    gCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaaca     <  1:1149772/93‑1 (MQ=255)
                                                                                     cACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAAGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaacaa    >  1:4385571/1‑93 (MQ=255)
                                                                                      aCCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGaacaac   <  1:4550057/93‑1 (MQ=255)
                                                                                       ccGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAcc  >  2:3836427/1‑93 (MQ=255)
                                                                                       ccGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAcc  <  1:1021191/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACC  >  NC_000913/1974961‑1975140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGT  >  NC_000913/1974959‑1975144
                                                                                            |                                                                                             
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGAC                                                                                              <  SRR4302543.4759187/94‑1 (MQ=60)
 GGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACA                                                                                             >  SRR4302543.560698/1‑94 (MQ=60)
  GAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAA                                                                                            <  SRR4302543.2520635/94‑1 (MQ=60)
   AGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAAT                                                                                           <  SRR4302543.6015024/94‑1 (MQ=60)
     CGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAA                                                                                         >  SRR4302543.4942652/1‑94 (MQ=60)
      GACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAAT                                                                                        <  SRR4302543.124391/94‑1 (MQ=60)
      GACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAAT                                                                                        <  SRR4302543.4284926/94‑1 (MQ=60)
       ACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATC                                                                                       <  SRR4302543.685032/94‑1 (MQ=60)
        CGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCA                                                                                      >  SRR4302543.7931784/1‑94 (MQ=60)
          AAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACC                                                                                    <  SRR4302543.7151854/94‑1 (MQ=60)
          AAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACC                                                                                    <  SRR4302543.5958013/94‑1 (MQ=60)
           AACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCA                                                                                   >  SRR4302543.1387950/1‑94 (MQ=60)
            ACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAG                                                                                  <  SRR4302543.3277669/94‑1 (MQ=60)
            ACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAG                                                                                  <  SRR4302543.2251452/94‑1 (MQ=60)
            ACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAG                                                                                  <  SRR4302543.4691952/94‑1 (MQ=60)
            ACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAG                                                                                  <  SRR4302543.6377201/94‑1 (MQ=60)
               GCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTC                                                                               <  SRR4302543.6777368/94‑1 (MQ=60)
               GCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTC                                                                               <  SRR4302543.4357252/94‑1 (MQ=60)
                 AACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAG                                                                             <  SRR4302543.1946682/94‑1 (MQ=60)
                 AACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAG                                                                             <  SRR4302543.1437682/94‑1 (MQ=60)
                 AACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAG                                                                             >  SRR4302543.1982220/1‑94 (MQ=60)
                   CGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCG                                                                           <  SRR4302543.355476/94‑1 (MQ=60)
                   CGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCG                                                                           >  SRR4302543.2196540/1‑94 (MQ=60)
                    GGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGG                                                                          >  SRR4302543.5330105/1‑94 (MQ=60)
                      CAGCGCCTTCAGCGTGCCTTTAATCGCTTTTCCATTATTGCCGACGATCAACGACCCAATCCTTGCACCGCCAATAATCACCAGTTCAGCGCGT                                                                        >  SRR4302543.1021956/1‑94 (MQ=60)
                      CAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGT                                                                        >  SRR4302543.1021957/1‑94 (MQ=60)
                       AGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTT                                                                       <  SRR4302543.181149/94‑1 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                     >  SRR4302543.4740070/1‑94 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                     <  SRR4302543.7015866/94‑1 (MQ=60)
                         CGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGA                                                                     <  SRR4302543.976390/94‑1 (MQ=60)
                          GCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAT                                                                    >  SRR4302543.6002382/1‑94 (MQ=60)
                          GCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAT                                                                    >  SRR4302543.5212807/1‑94 (MQ=60)
                          GCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAT                                                                    >  SRR4302543.5212805/1‑94 (MQ=60)
                          GCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCAACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGAT                                                                    <  SRR4302543.5281568/94‑1 (MQ=60)
                           CCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATA                                                                   >  SRR4302543.4597338/1‑94 (MQ=60)
                            CTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAG                                                                  >  SRR4302543.2788442/1‑94 (MQ=60)
                            CTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAG                                                                  >  SRR4302543.5464712/1‑94 (MQ=60)
                            CTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAG                                                                  >  SRR4302543.546154/1‑94 (MQ=60)
                            CTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAG                                                                  >  SRR4302543.3142823/1‑94 (MQ=60)
                            CTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAG                                                                  >  SRR4302543.293865/1‑94 (MQ=60)
                             TTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGCTCAGCGGGTTGATAGA                                                                 >  SRR4302543.1303739/1‑94 (MQ=60)
                              TCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAG                                                                <  SRR4302543.3000590/94‑1 (MQ=60)
                              TCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAG                                                                <  SRR4302543.5645658/94‑1 (MQ=60)
                               CAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGT                                                               >  SRR4302543.6873746/1‑94 (MQ=60)
                                AGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTG                                                              >  SRR4302543.4845082/1‑94 (MQ=60)
                                AGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTG                                                              >  SRR4302543.6732191/1‑94 (MQ=60)
                                 GCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTAATAGAGTGC                                                             <  SRR4302543.1562586/94‑1 (MQ=60)
                                 GCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGGTGATAGAGAGC                                                             >  SRR4302543.6429041/1‑94 (MQ=60)
                                  CGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCT                                                            <  SRR4302543.5294524/94‑1 (MQ=60)
                                  CGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCT                                                            >  SRR4302543.7772411/1‑94 (MQ=60)
                                  CGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCT                                                            >  SRR4302543.5492860/1‑94 (MQ=60)
                                   GTGCTTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTC                                                           <  SRR4302543.1651215/94‑1 (MQ=60)
                                   GTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTC                                                           >  SRR4302543.3727413/1‑94 (MQ=60)
                                      CCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAA                                                        >  SRR4302543.1679091/1‑94 (MQ=60)
                                       CTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAG                                                       <  SRR4302543.256870/94‑1 (MQ=60)
                                         TTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGC                                                     <  SRR4302543.1870134/94‑1 (MQ=60)
                                          TAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCT                                                    >  SRR4302543.2679674/1‑94 (MQ=60)
                                          TAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCT                                                    <  SRR4302543.466919/94‑1 (MQ=60)
                                          TAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCT                                                    <  SRR4302543.2086558/94‑1 (MQ=60)
                                           AATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTT                                                   >  SRR4302543.7488395/1‑94 (MQ=60)
                                             TCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCC                                                 >  SRR4302543.2435884/1‑94 (MQ=60)
                                              CGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCA                                                >  SRR4302543.4154261/1‑94 (MQ=60)
                                              CGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCA                                                >  SRR4302543.7021008/1‑94 (MQ=60)
                                              CGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCA                                                >  SRR4302543.4979264/1‑94 (MQ=60)
                                              CGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCA                                                >  SRR4302543.278426/1‑94 (MQ=60)
                                                CTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACC                                              <  SRR4302543.6892217/94‑1 (MQ=60)
                                                CTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACC                                              <  SRR4302543.5704202/94‑1 (MQ=60)
                                                CTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACC                                              <  SRR4302543.5704206/94‑1 (MQ=60)
                                                 TTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCG                                             <  SRR4302543.6643201/94‑1 (MQ=60)
                                                   TGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGCTAGAGTGCTCCAAGGCTTCCACCGGT                                           >  SRR4302543.5354949/1‑94 (MQ=60)
                                                   TGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGT                                           >  SRR4302543.5354950/1‑94 (MQ=60)
                                                   TGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGC                                           >  SRR4302543.445824/1‑94 (MQ=60)
                                                    GCCATTATTGCCGACGATAAATGACCCAATTCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCAACGGTC                                          >  SRR4302543.6521972/1‑94 (MQ=60)
                                                     CCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCA                                         <  SRR4302543.5108366/94‑1 (MQ=60)
                                                     CCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCA                                         >  SRR4302543.5976670/1‑94 (MQ=60)
                                                     CCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCA                                         <  SRR4302543.955751/94‑1 (MQ=60)
                                                      CATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCAT                                        <  SRR4302543.5622426/94‑1 (MQ=60)
                                                       ATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATC                                       >  SRR4302543.4261797/1‑94 (MQ=60)
                                                       ATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATC                                       >  SRR4302543.5375930/1‑94 (MQ=60)
                                                       ATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATC                                       >  SRR4302543.4828557/1‑94 (MQ=60)
                                                       ATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATC                                       >  SRR4302543.462721/1‑94 (MQ=60)
                                                       ATTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTAATC                                       >  SRR4302543.6270780/1‑94 (MQ=60)
                                                        TTATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCA                                      <  SRR4302543.7086011/94‑1 (MQ=60)
                                                         TATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAA                                     >  SRR4302543.574058/1‑94 (MQ=60)
                                                          ATTGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAA                                    >  SRR4302543.6344594/1‑94 (MQ=60)
                                                            TGCCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATA                                  >  SRR4302543.4646092/1‑94 (MQ=60)
                                                              CCGACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAAC                                >  SRR4302543.4757116/1‑94 (MQ=60)
                                                                GACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCG                              >  SRR4302543.7801975/1‑94 (MQ=60)
                                                                GACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCG                              <  SRR4302543.6845979/94‑1 (MQ=60)
                                                                CACGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCG                              <  SRR4302543.1257660/94‑1 (MQ=60)
                                                                  CGATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCGCCGGTCATCAAATAACCGCC                            <  SRR4302543.7805039/94‑1 (MQ=60)
                                                                    ATAAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGA                          >  SRR4302543.5685551/1‑94 (MQ=60)
                                                                      AAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAA                        >  SRR4302543.7374968/1‑94 (MQ=60)
                                                                      AAACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAA                        >  SRR4302543.4149439/1‑94 (MQ=60)
                                                                       AACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAA                       <  SRR4302543.3367578/94‑1 (MQ=60)
                                                                       AACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAA                       >  SRR4302543.1509423/1‑94 (MQ=60)
                                                                       AACGACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAA                       >  SRR4302543.1838360/1‑94 (MQ=60)
                                                                          GACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTG                    >  SRR4302543.5177399/1‑94 (MQ=60)
                                                                           ACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGA                   >  SRR4302543.5806799/1‑94 (MQ=60)
                                                                           ACCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGT                   >  SRR4302543.3562058/1‑94 (MQ=60)
                                                                            CCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTA                  <  SRR4302543.3600842/94‑1 (MQ=60)
                                                                            CCCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTA                  <  SRR4302543.3600843/94‑1 (MQ=60)
                                                                             CCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAC                 <  SRR4302543.6112802/94‑1 (MQ=60)
                                                                             CCAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTAC                 >  SRR4302543.2656260/1‑94 (MQ=60)
                                                                              CAATCCCTGCACCGACACTAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC                <  SRR4302543.7104120/94‑1 (MQ=60)
                                                                              CAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC                >  SRR4302543.6310632/1‑94 (MQ=60)
                                                                              CAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC                <  SRR4302543.4821927/94‑1 (MQ=60)
                                                                              CAATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACC                >  SRR4302543.6310633/1‑94 (MQ=60)
                                                                               AATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCG               >  SRR4302543.4792076/1‑94 (MQ=60)
                                                                                ATCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGA              <  SRR4302543.1125250/94‑1 (MQ=60)
                                                                                 TCCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAG             <  SRR4302543.3746848/94‑1 (MQ=60)
                                                                                  CCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGA            >  SRR4302543.2890921/1‑94 (MQ=60)
                                                                                  CCCTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGA            >  SRR4302543.7298890/1‑94 (MQ=60)
                                                                                    CTGCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAAC          <  SRR4302543.1967099/94‑1 (MQ=60)
                                                                                      GCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAA        <  SRR4302543.1160250/94‑1 (MQ=60)
                                                                                      GCACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAAGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAA        >  SRR4302543.4425336/1‑94 (MQ=60)
                                                                                       CACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAAC       >  SRR4302543.7021386/1‑94 (MQ=60)
                                                                                        ACCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACC      <  SRR4302543.4591496/94‑1 (MQ=60)
                                                                                         CCGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCA     <  SRR4302543.1030227/94‑1 (MQ=60)
                                                                                          CGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAG    >  SRR4302543.2040715/1‑94 (MQ=60)
                                                                                          CGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAG    <  SRR4302543.1768508/94‑1 (MQ=60)
                                                                                          CGACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGACATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAG    >  SRR4302543.3725285/1‑94 (MQ=60)
                                                                                           GACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGG   >  SRR4302543.7293469/1‑94 (MQ=60)
                                                                                            ACAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACAGCCGAAAAGTGTATCGTGAACAACCAGGT  >  SRR4302543.7211053/1‑94 (MQ=60)
                                                                                            |                                                                                             
TGGAGCGACGAAACAGCAACGGCAGCGCCTTCAGCGTGCCTTTAATCGCTTTGCCATTATTGCCGACGATAAACGACCCAATCCCTGCACCGGCAATAATCACCAGTTCAGCGGGTTGATAGAGTGCTCCAAGGCTTCCACCGGTCATCAAATAACCGCCGAAAACTGTACCGAGAACAACCAGGT  >  NC_000913/1974959‑1975144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑