Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,999,772 T→C H4R (CAC→CGC)  b1922 ← sigma factor 28

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,999,7720TC100.0% 705.2 / NA 226H4R (CAC→CGC) b1922sigma factor 28
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (91/135);  total (91/135)

Only 100 of 240 total aligned reads displayed.

AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999682‑1999861
                                                                                          |                                                                                         
aGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCGGCGGGACATAACGCTGCCCCAGCGAgcgt                                                                                         >  1:6042473/1‑93 (MQ=255)
  aTCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGttt                                                                                       >  1:1108663/1‑93 (MQ=255)
   tCGTCAAGTTCGACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  2:2038149/93‑1 (MQ=255)
   tCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      >  1:1755523/1‑93 (MQ=255)
   tCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTa                                                                                      <  1:2582527/93‑1 (MQ=255)
    cGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAt                                                                                     <  1:3411901/93‑1 (MQ=255)
     gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    >  1:6186772/1‑93 (MQ=255)
     gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    >  1:1653013/1‑93 (MQ=255)
     gTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATc                                                                                    <  1:3796418/93‑1 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  2:2753771/93‑1 (MQ=255)
       cAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCa                                                                                  <  2:1947372/93‑1 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 >  2:2813779/1‑93 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 <  2:7208187/93‑1 (MQ=255)
        aaGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAt                                                                                 >  1:5148112/1‑93 (MQ=255)
         aGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAtt                                                                                >  1:4137408/1‑93 (MQ=255)
          gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               >  1:4993914/1‑93 (MQ=255)
          gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               <  1:6972819/93‑1 (MQ=255)
          gTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTa                                                                               <  2:531260/93‑1 (MQ=255)
            tCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTaca                                                                             <  2:3368239/93‑1 (MQ=255)
            tCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTaca                                                                             <  1:4537410/93‑1 (MQ=255)
            tCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTaca                                                                             <  1:5130263/93‑1 (MQ=255)
             ccACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTacac                                                                            <  1:3527363/93‑1 (MQ=255)
             ccACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTacac                                                                            <  1:5243142/93‑1 (MQ=255)
              cACGCTCGCGGGCAGTCGAACGTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAcc                                                                           >  1:1367618/1‑93 (MQ=255)
               aCGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCt                                                                          >  1:6280014/1‑93 (MQ=255)
                  cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       <  2:4859332/93‑1 (MQ=255)
                  cTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCa                                                                       <  2:5695753/93‑1 (MQ=255)
                   tCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAg                                                                      >  2:18571/1‑93 (MQ=255)
                   tCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAg                                                                      >  2:5894878/1‑93 (MQ=255)
                    cgcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGc                                                                     <  1:1003197/93‑1 (MQ=255)
                     gcgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCg                                                                    <  1:7774476/93‑1 (MQ=255)
                      cgGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCgg                                                                   <  2:5024061/93‑1 (MQ=255)
                        ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGta                                                                 <  1:6660323/93‑1 (MQ=255)
                        ggCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGta                                                                 <  1:3626588/93‑1 (MQ=255)
                         gCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtat                                                                <  2:6156409/93‑1 (MQ=255)
                         gCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtat                                                                >  2:6614689/1‑93 (MQ=255)
                          cAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtata                                                               >  1:4953601/1‑93 (MQ=255)
                          cAGTCGAACCTGCAGGCGCAATGCTGCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGtata                                                               <  1:2612477/93‑1 (MQ=255)
                           aGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATag                                                              <  2:1702326/93‑1 (MQ=255)
                            gTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATaga                                                             <  2:7058631/93‑1 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            >  2:4647734/1‑93 (MQ=255)
                             tCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATagag                                                            <  2:6897742/93‑1 (MQ=255)
                              cGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGt                                                           <  2:2710186/93‑1 (MQ=255)
                               gAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTg                                                          >  1:2892038/1‑93 (MQ=255)
                               gAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTg                                                          >  1:233825/1‑93 (MQ=255)
                                aaCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGa                                                         <  1:4312502/93‑1 (MQ=255)
                                aaCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGa                                                         <  2:4545594/93‑1 (MQ=255)
                                aaCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGa                                                         >  2:3893677/1‑93 (MQ=255)
                                 aCCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGaa                                                        >  2:130655/1‑93 (MQ=255)
                                   cTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAtt                                                      <  2:612733/93‑1 (MQ=255)
                                   cTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAAtt                                                      <  2:4294496/93‑1 (MQ=255)
                                     gCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCa                                                    <  1:5324747/93‑1 (MQ=255)
                                      cAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCAc                                                   <  1:650099/93‑1 (MQ=255)
                                           gcAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaa                                              >  2:4830705/1‑93 (MQ=255)
                                            cAATGCTTCGTGACTGACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCCTTACGCCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             <  2:421812/93‑1 (MQ=255)
                                            cAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             <  1:2987881/93‑1 (MQ=255)
                                            cAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATaaa                                             >  1:5808595/1‑93 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            <  2:2327210/93‑1 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            <  1:4398321/93‑1 (MQ=255)
                                             aaTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAc                                            <  2:7443295/93‑1 (MQ=255)
                                              aTGCTTCGTGACGCAGCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACa                                           <  2:665683/93‑1 (MQ=255)
                                              aTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACa                                           >  1:882314/1‑93 (MQ=255)
                                                gCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc                                         <  1:128814/93‑1 (MQ=255)
                                                gCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc                                         <  1:6432083/93‑1 (MQ=255)
                                                gCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGc                                         >  1:5374497/1‑93 (MQ=255)
                                                     gTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGc                                    >  2:5180824/1‑93 (MQ=255)
                                                         cGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTta                                >  2:1387645/1‑93 (MQ=255)
                                                           cACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtata                              <  2:2255994/93‑1 (MQ=255)
                                                           cACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTtata                              <  2:4124903/93‑1 (MQ=255)
                                                             ccAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATg                            <  2:3269184/93‑1 (MQ=255)
                                                              cAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa                           <  2:5612598/93‑1 (MQ=255)
                                                              cAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGa                           <  1:2231695/93‑1 (MQ=255)
                                                               aGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAg                          >  1:4903661/1‑93 (MQ=255)
                                                                gCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGt                         <  1:2097009/93‑1 (MQ=255)
                                                                 cGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGtt                        <  1:1498074/93‑1 (MQ=255)
                                                                   ggACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTAt                      <  1:632269/93‑1 (MQ=255)
                                                                   gcaCATAACGCTGCCACAGCGAGCGTTTATACATTACCCCTTCAGCGGTATAGAGTGAATTCACCATAACCAGCCCTGCGTTATATGAGTTAt                      <  2:7726102/91‑1 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:230206/93‑1 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     >  1:242331/1‑93 (MQ=255)
                                                                    gACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATc                     <  2:6196698/93‑1 (MQ=255)
                                                                        tAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCa                 <  1:7286482/93‑1 (MQ=255)
                                                                          aCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATg               >  1:705860/1‑93 (MQ=255)
                                                                           cGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGa              >  1:6910219/1‑93 (MQ=255)
                                                                            gCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAt             >  2:5955874/1‑93 (MQ=255)
                                                                             cTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGAtt            <  2:2201963/93‑1 (MQ=255)
                                                                               gCCACAGCGAGCGTCTATCCATTACACCTCCAGCGGTAGAGAGTGAAGTCACGAGAACCAGCCCTGCGTTATAGGAGTTTTCGGCAGGATTAt          <  2:4269576/93‑1 (MQ=255)
                                                                                ccACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATc         <  1:718070/93‑1 (MQ=255)
                                                                                ccACAGCGAGCGTGTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACATCCCTGCGTTATATGAGTTATCGGCATTATTATc         <  2:4115952/93‑1 (MQ=255)
                                                                                 cacaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATcc        >  1:2133169/1‑93 (MQ=255)
                                                                                  acaGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCg       >  1:2472310/1‑93 (MQ=255)
                                                                                    agcgagcgTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGtt     >  2:5825806/1‑93 (MQ=255)
                                                                                     gCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAAGAGTTATCGGCATGATTATCCGttt    <  2:7379747/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  2:7756380/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  2:1222558/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:635726/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:203868/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTc   <  1:1691664/93‑1 (MQ=255)
                                                                                      cGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCCTTTc   <  1:429543/93‑1 (MQ=255)
                                                                                       gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  1:476115/1‑93 (MQ=255)
                                                                                       gAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCt  >  1:1030213/1‑93 (MQ=255)
                                                                                          |                                                                                         
AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT  >  NC_000913/1999682‑1999861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999680‑1999865
                                                                                            |                                                                                             
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCG                                                                                              <  SRR4302543.3728463/94‑1 (MQ=60)
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCG                                                                                              <  SRR4302543.1704248/94‑1 (MQ=60)
 CAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCGGCGGGACATAACGCTGCCCCAGCGAGCGT                                                                                             >  SRR4302543.6099662/1‑94 (MQ=60)
 CAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGT                                                                                             <  SRR4302543.7044823/94‑1 (MQ=60)
  AGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTT                                                                                            >  SRR4302543.934228/1‑94 (MQ=60)
   GATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTT                                                                                           >  SRR4302543.1118668/1‑94 (MQ=60)
    ATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTA                                                                                          >  SRR4302543.1771203/1‑94 (MQ=60)
     TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         <  SRR4302543.2606028/94‑1 (MQ=60)
     TCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTAT                                                                                         <  SRR4302543.1398243/94‑1 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        <  SRR4302543.54473/94‑1 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        <  SRR4302543.4879480/94‑1 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302543.1667801/1‑94 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        <  SRR4302543.3442445/94‑1 (MQ=60)
      CGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATC                                                                                        >  SRR4302543.6245231/1‑94 (MQ=60)
       GTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCC                                                                                       >  SRR4302543.6494526/1‑94 (MQ=60)
       GTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCC                                                                                       <  SRR4302543.3829862/94‑1 (MQ=60)
         CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302543.2493102/94‑1 (MQ=60)
         CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     >  SRR4302543.5194649/1‑94 (MQ=60)
         CAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCAT                                                                                     <  SRR4302543.5160440/94‑1 (MQ=60)
          AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    >  SRR4302543.6346132/1‑94 (MQ=60)
          AAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATT                                                                                    >  SRR4302543.4174759/1‑94 (MQ=60)
           AGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTA                                                                                   >  SRR4302543.6409459/1‑94 (MQ=60)
           AGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTA                                                                                   >  SRR4302543.5039375/1‑94 (MQ=60)
            GTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAC                                                                                  <  SRR4302543.5243838/94‑1 (MQ=60)
            GTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTAC                                                                                  <  SRR4302543.7038436/94‑1 (MQ=60)
             TTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACA                                                                                 >  SRR4302543.4892658/1‑94 (MQ=60)
              TCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAC                                                                                <  SRR4302543.5176630/94‑1 (MQ=60)
              TCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACAC                                                                                <  SRR4302543.4578714/94‑1 (MQ=60)
               CCACGCTCGCGGGCGGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               <  SRR4302543.3558803/94‑1 (MQ=60)
               CCACGCTCGCGGGCAGTCGAACGTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               >  SRR4302543.1379883/1‑94 (MQ=60)
               CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               <  SRR4302543.3558802/94‑1 (MQ=60)
               CCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACC                                                                               <  SRR4302543.5290400/94‑1 (MQ=60)
                CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              <  SRR4302543.7297026/94‑1 (MQ=60)
                CACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCT                                                                              >  SRR4302543.6339201/1‑94 (MQ=60)
                   GCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCA                                                                           <  SRR4302543.1696773/94‑1 (MQ=60)
                   GCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCA                                                                           <  SRR4302543.4955754/94‑1 (MQ=60)
                    CTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                          >  SRR4302543.6034735/1‑94 (MQ=60)
                    CTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAG                                                                          >  SRR4302543.2104654/1‑94 (MQ=60)
                      CGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCG                                                                        <  SRR4302543.1012065/94‑1 (MQ=60)
                      CGCCGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCG                                                                        <  SRR4302543.1715040/94‑1 (MQ=60)
                       GCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGG                                                                       <  SRR4302543.7847801/94‑1 (MQ=60)
                          GGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTAT                                                                    <  SRR4302543.3658527/94‑1 (MQ=60)
                          GGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTAT                                                                    <  SRR4302543.6723201/94‑1 (MQ=60)
                           GCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATA                                                                   >  SRR4302543.4998710/1‑94 (MQ=60)
                            CAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  <  SRR4302543.235895/94‑1 (MQ=60)
                            CAGTCGAACCTGCAGGCGCAATGCTGCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAG                                                                  <  SRR4302543.2636178/94‑1 (MQ=60)
                              GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                >  SRR4302543.1327804/1‑94 (MQ=60)
                              GTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAG                                                                <  SRR4302543.3044305/94‑1 (MQ=60)
                               TCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGT                                                               >  SRR4302543.2098414/1‑94 (MQ=60)
                               TCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGT                                                               <  SRR4302543.1494042/94‑1 (MQ=60)
                               TCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGT                                                               >  SRR4302543.7038174/1‑94 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              >  SRR4302543.235844/1‑94 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              <  SRR4302543.6250653/94‑1 (MQ=60)
                                CGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTG                                                              >  SRR4302543.2918341/1‑94 (MQ=60)
                                 GAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCATCGGTATAGAGTGA                                                             >  SRR4302543.466190/1‑94 (MQ=60)
                                  AACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAA                                                            <  SRR4302543.4351675/94‑1 (MQ=60)
                                   ACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGGTTATCCATTACACCTTCAGCGGTATAGAGTGAAT                                                           >  SRR4302543.5780231/1‑94 (MQ=60)
                                    CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          >  SRR4302543.1715738/1‑94 (MQ=60)
                                    CCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATT                                                          <  SRR4302543.1213720/94‑1 (MQ=60)
                                      TGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCA                                                        >  SRR4302543.2341631/1‑94 (MQ=60)
                                       GCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCAC                                                       <  SRR4302543.5372889/94‑1 (MQ=60)
                                       GCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCAC                                                       <  SRR4302543.5372891/94‑1 (MQ=60)
                                        CAGGCGCACTGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      <  SRR4302543.2893533/94‑1 (MQ=60)
                                        CAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACG                                                      <  SRR4302543.656047/94‑1 (MQ=60)
                                         AGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGA                                                     >  SRR4302543.2921828/1‑94 (MQ=60)
                                          GGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGAT                                                    >  SRR4302543.1613832/1‑94 (MQ=60)
                                            CGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAA                                                  <  SRR4302543.5179131/94‑1 (MQ=60)
                                             GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 >  SRR4302543.3958517/1‑94 (MQ=60)
                                             GCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAA                                                 >  SRR4302543.5861422/1‑94 (MQ=60)
                                              CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                >  SRR4302543.7906116/1‑94 (MQ=60)
                                              CAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAAC                                                <  SRR4302543.3015014/94‑1 (MQ=60)
                                               AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               <  SRR4302543.4438191/94‑1 (MQ=60)
                                               AATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACA                                               >  SRR4302543.890138/1‑94 (MQ=60)
                                                ATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAG                                              <  SRR4302543.4299380/94‑1 (MQ=60)
                                                 TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             >  SRR4302543.5423179/1‑94 (MQ=60)
                                                 TGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGC                                             >  SRR4302543.723682/1‑94 (MQ=60)
                                                  GCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCC                                            <  SRR4302543.6492688/94‑1 (MQ=60)
                                                  GCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCC                                            <  SRR4302543.129967/94‑1 (MQ=60)
                                                  GCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGGATAGAGAGAATTCACGATAAACAGCC                                            >  SRR4302543.6797552/1‑94 (MQ=60)
                                                   CTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCC                                           <  SRR4302543.6108330/94‑1 (MQ=60)
                                                    TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302543.2130698/94‑1 (MQ=60)
                                                    TTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCT                                          <  SRR4302543.3745242/94‑1 (MQ=60)
                                                     TCGTGCCGCCCCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTCCACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTG                                         <  SRR4302543.4508800/94‑1 (MQ=60)
                                                      CGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGC                                        >  SRR4302543.823558/1‑94 (MQ=60)
                                                      CGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGC                                        >  SRR4302543.4349051/1‑94 (MQ=60)
                                                       GTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCG                                       >  SRR4302543.504756/1‑94 (MQ=60)
                                                           CGCACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTAT                                   >  SRR4302543.4409272/1‑94 (MQ=60)
                                                             CACGAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 <  SRR4302543.5012322/94‑1 (MQ=60)
                                                             CACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 >  SRR4302543.6415121/1‑94 (MQ=60)
                                                             CACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 <  SRR4302543.4482320/94‑1 (MQ=60)
                                                             CACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATAT                                 <  SRR4302543.1188810/94‑1 (MQ=60)
                                                              ACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATG                                >  SRR4302543.2309857/1‑94 (MQ=60)
                                                              ACCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATG                                >  SRR4302543.6499745/1‑94 (MQ=60)
                                                               CCAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGA                               >  SRR4302543.7733755/1‑94 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                              <  SRR4302543.6572741/94‑1 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                              >  SRR4302543.7014968/1‑94 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                              <  SRR4302543.2251867/94‑1 (MQ=60)
                                                                CAGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAG                              >  SRR4302543.4948354/1‑94 (MQ=60)
                                                                 CGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                             <  SRR4302543.1090416/94‑1 (MQ=60)
                                                                 AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                             >  SRR4302543.2634672/1‑94 (MQ=60)
                                                                 AGCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGT                             <  SRR4302543.2489123/94‑1 (MQ=60)
                                                                  GCGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTT                            <  SRR4302543.2115754/94‑1 (MQ=60)
                                                                   CGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTA                           <  SRR4302543.3662779/94‑1 (MQ=60)
                                                                   CGGGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTA                           <  SRR4302543.1511549/94‑1 (MQ=60)
                                                                     GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302543.638045/94‑1 (MQ=60)
                                                                     GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         <  SRR4302543.3163047/94‑1 (MQ=60)
                                                                     GGACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATC                         >  SRR4302543.244433/1‑94 (MQ=60)
                                                                       ACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       >  SRR4302543.2906545/1‑94 (MQ=60)
                                                                       ACATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGG                       >  SRR4302543.5414505/1‑94 (MQ=60)
                                                                        CATAACGCTGCCACAGCGAGCGTTTATCCATTATACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGC                      >  SRR4302543.4189788/1‑94 (MQ=60)
                                                                         ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCA                     >  SRR4302543.5224313/1‑94 (MQ=60)
                                                                         ATAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGATATATGAGTTATCGGCA                     >  SRR4302543.7085670/1‑94 (MQ=60)
                                                                          TAACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCAT                    <  SRR4302543.7356830/94‑1 (MQ=60)
                                                                           ACCGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATTGGCATG                   <  SRR4302543.249414/94‑1 (MQ=60)
                                                                           AACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATG                   >  SRR4302543.712289/1‑94 (MQ=60)
                                                                            ACGCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGA                  >  SRR4302543.6975165/1‑94 (MQ=60)
                                                                              GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                >  SRR4302543.2152409/1‑94 (MQ=60)
                                                                              GCTGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATT                <  SRR4302543.6208507/94‑1 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              <  SRR4302543.3465729/94‑1 (MQ=60)
                                                                                TGCCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTAT              <  SRR4302543.2041986/94‑1 (MQ=60)
                                                                                  CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            >  SRR4302543.2152267/1‑94 (MQ=60)
                                                                                  CCACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCC            <  SRR4302543.724561/94‑1 (MQ=60)
                                                                                   CACAGCGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCG           >  SRR4302543.2494635/1‑94 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      >  SRR4302543.1039350/1‑94 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302543.1706846/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302543.7250265/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302543.205592/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      <  SRR4302543.641538/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      >  SRR4302543.480516/1‑94 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCCTTTCT      <  SRR4302543.433568/94‑1 (MQ=60)
                                                                                        CGAGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGACCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCT      >  SRR4302543.1039349/1‑94 (MQ=60)
                                                                                          AGCGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGC    <  SRR4302543.575582/94‑1 (MQ=60)
                                                                                            CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  SRR4302543.7893669/1‑94 (MQ=60)
                                                                                            CGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  <  SRR4302543.6679775/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCAGATCGTCAAGTTCCACGCTCGCGGGCAGTCGAACCTGCAGGCGCAATGCTTCGTGACGCACCAGCGGGACATAACGCTGCCACAGCGAGTGTTTATCCATTACACCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAG  >  NC_000913/1999680‑1999865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑