Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,976	A→G	E3E (GAA→GAG)	fliJ →	FliJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,976	0	A	G	99.1%	688.5 / ‑3.6	219	E3E (GAA→GAG)	fliJ	FliJ
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (117/100); total (118/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 227 total aligned reads displayed.

TGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCA  >  NC_000913/2015886‑2016065

tGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCa                                                                                         >  1:7375236/1‑93 (MQ=255)

tGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCa                                                                                         <  1:223118/93‑1 (MQ=255)

 taccgcgcgGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATACCCCAGGAGATAACGGCAGTTGGCAGAGCAt                                                                                        <  2:4014859/90‑1 (MQ=255)

 gaccgcgcgGACTGGGAAGCGTCTCTCCAGGTGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCAt                                                                                        <  1:3108550/90‑1 (MQ=255)

 gAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCag                                                                                        >  2:4505739/1‑92 (MQ=255)

 gAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCAt                                                                                        >  2:5169983/1‑93 (MQ=255)

  aaCGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATg                                                                                       <  1:4898583/93‑1 (MQ=255)

  aaCGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATg                                                                                       <  1:4912280/93‑1 (MQ=255)

  aaCGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATg                                                                                       <  1:5207031/93‑1 (MQ=255)

        cgGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTgcgc                                                                                 <  1:2092625/93‑1 (MQ=255)

         ggACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTgcgcg                                                                                >  1:3214577/1‑92 (MQ=255)

         ggACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCt                                                                                >  1:7796214/1‑93 (MQ=255)

          gACTGGGAAGCGTCCCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTg                                                                               <  1:2711945/93‑1 (MQ=255)

            cTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGc                                                                             >  1:7934192/1‑93 (MQ=255)

             tGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGGGACATAACCCAGGAGATAACGGCACATGGCACAGCATGGGGGGCTcgcg                                                                            >  2:3771512/1‑93 (MQ=255)

               ggAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGCGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGAc                                                                          <  1:5024439/93‑1 (MQ=255)

                      tctctcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGaaa                                                                   <  1:5403219/93‑1 (MQ=255)

                      tctctcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGaaa                                                                   <  2:7412667/93‑1 (MQ=255)

                      tctctcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGaaa                                                                   <  1:7420237/93‑1 (MQ=255)

                      tctctcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGaaa                                                                   <  2:5435949/93‑1 (MQ=255)

                          tcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATc                                                               >  1:7556019/1‑93 (MQ=255)

                          tcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATc                                                               <  2:2688908/93‑1 (MQ=255)

                          tcCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATc                                                               >  2:6941322/1‑93 (MQ=255)

                           ccAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCt                                                              <  2:1690459/93‑1 (MQ=255)

                           ccAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGGGCGCTGGCGACCCTGAAAGATCt                                                              >  1:176537/1‑93 (MQ=255)

                            cAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTg                                                             <  1:1616743/93‑1 (MQ=255)

                            cAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTg                                                             >  1:3207902/1‑93 (MQ=255)

                             aGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTgg                                                            >  2:3542297/1‑93 (MQ=255)

                              ggggCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGc                                                           <  2:3698630/93‑1 (MQ=255)

                                  cTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaa                                                       <  2:1137276/93‑1 (MQ=255)

                                   tGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaa                                                      <  1:1724383/93‑1 (MQ=255)

                                   tGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGAGACCCTGAAAGATCTGGCAGcaa                                                      >  1:4425321/1‑93 (MQ=255)

                                    ggAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaa                                                     >  2:1380350/1‑93 (MQ=255)

                                     gAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa                                                    >  2:4082813/1‑93 (MQ=255)

                                     gAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa                                                    <  1:3698506/93‑1 (MQ=255)

                                     gAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa                                                    >  2:3063965/1‑93 (MQ=255)

                                     gAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa                                                    >  1:1335549/1‑93 (MQ=255)

                                     gAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa                                                    <  1:654319/93‑1 (MQ=255)

                                      aGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAag                                                   >  2:4418186/1‑93 (MQ=255)

                                      aGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAag                                                   >  1:7093947/1‑93 (MQ=255)

                                       gCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAaga                                                  <  2:6566584/93‑1 (MQ=255)

                                       gCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAaga                                                  <  2:78377/93‑1 (MQ=255)

                                       gCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAaga                                                  >  1:1424118/1‑93 (MQ=255)

                                       gCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAaga                                                  <  1:4011502/93‑1 (MQ=255)

                                        cGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAagag                                                 >  1:6283685/1‑93 (MQ=255)

                                        cGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAagag                                                 >  1:2041344/1‑93 (MQ=255)

                                        cGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAagag                                                 <  2:2468685/93‑1 (MQ=255)

                                        cGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAagag                                                 >  1:5980781/1‑93 (MQ=255)

                                          tATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTTGCAGAAAAAGAggg                                               >  1:2705393/1‑92 (MQ=255)

                                          tATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAgga                                               >  2:7168927/1‑92 (MQ=255)

                                          tATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAgga                                               >  2:5954600/1‑92 (MQ=255)

                                           aTTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTa                                              >  2:3211775/1‑93 (MQ=255)

                                           aTTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTa                                              >  1:2905035/1‑93 (MQ=255)

                                           aTTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTa                                              >  1:5295038/1‑93 (MQ=255)

                                            ttttCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTag                                             >  1:4259060/1‑93 (MQ=255)

                                             tttCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTaga                                            <  2:6587958/93‑1 (MQ=255)

                                              ttCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTagag                                           <  1:6948498/93‑1 (MQ=255)

                                              ttCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTagag                                           >  2:2245891/1‑93 (MQ=255)

                                               tCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAgg                                          >  1:7765842/1‑93 (MQ=255)

                                               tCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAgg                                          <  1:5775076/93‑1 (MQ=255)

                                                cccGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGa                                         <  2:1040699/93‑1 (MQ=255)

                                                  cGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATg                                       >  1:3481028/1‑93 (MQ=255)

                                                   gACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGc                                      >  2:6443862/1‑93 (MQ=255)

                                                   gACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGc                                      >  1:1858148/1‑93 (MQ=255)

                                                   gACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGc                                      >  1:5042769/1‑93 (MQ=255)

                                                    aCAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGcc                                     >  2:7326096/1‑93 (MQ=255)

                                                     cAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCcg                                    >  2:5718886/1‑93 (MQ=255)

                                                     cAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCcg                                    >  2:318760/1‑93 (MQ=255)

                                                       gtgtCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCcgcg                                  >  1:4625170/1‑93 (MQ=255)

                                                        tgtCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCcgcgc                                 >  1:3924589/1‑93 (MQ=255)

                                                           cATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGcc                              <  1:7833845/93‑1 (MQ=255)

                                                            aTAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCct                             >  2:1084214/1‑93 (MQ=255)

                                                               aCCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCctgct                          <  2:870083/93‑1 (MQ=255)

                                                               aCCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCctgct                          <  2:305888/93‑1 (MQ=255)

                                                                cccAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCctgctg                         <  2:3972451/93‑1 (MQ=255)

                                                                cccAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCctgctg                         <  2:7105426/93‑1 (MQ=255)

                                                                cccAGGAAATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCctgctg                         >  2:2042891/1‑93 (MQ=255)

                                                                  cAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTggg                       >  2:4297362/1‑93 (MQ=255)

                                                                  cAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTggg                       >  2:1919088/1‑93 (MQ=255)

                                                                   aGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGt                      >  2:3303132/1‑93 (MQ=255)

                                                                     gagaTAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGa                    >  1:4828549/1‑93 (MQ=255)

                                                                      agaTAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGaa                   >  2:6197950/1‑93 (MQ=255)

                                                                       gaTAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGaaa                  >  2:4910179/1‑93 (MQ=255)

                                                                       gaTAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGaaa                  <  1:1125434/93‑1 (MQ=255)

                                                                         tAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATg                <  1:2555900/93‑1 (MQ=255)

                                                                           aCGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCg              >  2:3881662/1‑93 (MQ=255)

                                                                            cGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGt             <  2:2801974/93‑1 (MQ=255)

                                                                            cGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGt             <  2:692596/93‑1 (MQ=255)

                                                                             ggCAGATGGCGGGGCGTGGTGCGCTGGCGCCGCTGAAAGATCTGGCAGAAAAGGGGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTc            <  2:6999463/93‑1 (MQ=255)

                                                                               cAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTcgc          >  2:7767640/1‑93 (MQ=255)

                                                                                 gATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCgg        >  1:1049671/1‑93 (MQ=255)

                                                                                 gATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCgg        >  1:5160786/1‑93 (MQ=255)

                                                                                 gATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCgg        >  2:648095/1‑93 (MQ=255)

                                                                                  aTGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGCAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGa       <  2:7026846/93‑1 (MQ=255)

                                                                                  aTGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGa       <  1:549301/93‑1 (MQ=255)

                                                                                   tGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGAt      <  2:5897996/93‑1 (MQ=255)

                                                                                   tGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGAt      >  2:3235718/1‑93 (MQ=255)

                                                                                   tGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGAt      <  2:3551361/93‑1 (MQ=255)

                                                                                    ggCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATg     <  1:4835096/93‑1 (MQ=255)

                                                                                      cAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTc   <  1:3896526/93‑1 (MQ=255)

TGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCA  >  NC_000913/2015886‑2016065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAGCAG  >  NC_000913/2015883‑2016069

TTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAG                                                                                               >  SRR4302543.946966/1‑94 (MQ=60)

TTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAG                                                                                               <  SRR4302543.5698003/94‑1 (MQ=60)

  TTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCA                                                                                             <  SRR4302543.957573/94‑1 (MQ=60)

  TTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCA                                                                                             >  SRR4302543.7446133/1‑94 (MQ=60)

  TTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCA                                                                                             <  SRR4302543.5390339/94‑1 (MQ=60)

   TGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCAT                                                                                            <  SRR4302543.225045/94‑1 (MQ=60)

    GACCGCGCGGACTGGGAAGCGTCTCTCCAGGTGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATG                                                                                           <  SRR4302543.3136515/94‑1 (MQ=60)

    GAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATG                                                                                           >  SRR4302543.1930126/1‑94 (MQ=60)

    GAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATG                                                                                           >  SRR4302543.606347/1‑94 (MQ=60)

     AACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGG                                                                                          <  SRR4302543.4957051/94‑1 (MQ=60)

     AACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGG                                                                                          <  SRR4302543.4943234/94‑1 (MQ=60)

     AACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGG                                                                                          <  SRR4302543.5253959/94‑1 (MQ=60)

     AACGCGCGGACTGGGAAGCGTCGCTCCAGGGGCTGGATCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGG                                                                                          <  SRR4302543.4688775/94‑1 (MQ=60)

      ACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGT                                                                                         <  SRR4302543.3310231/94‑1 (MQ=60)

        GCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGC                                                                                       <  SRR4302543.4955956/94‑1 (MQ=60)

           CGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCT                                                                                    >  SRR4302543.7869680/1‑94 (MQ=60)

           CGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCT                                                                                    <  SRR4302543.2111320/94‑1 (MQ=60)

           CGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCG                                                                                    >  SRR4302543.3243609/1‑94 (MQ=60)

             GACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGG                                                                                  <  SRR4302543.5304201/94‑1 (MQ=60)

             GACTGGGAAGCGTCCCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGG                                                                                  <  SRR4302543.2736581/94‑1 (MQ=60)

             GACGGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGG                                                                                  <  SRR4302543.2736580/94‑1 (MQ=60)

              ACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGC                                                                                 >  SRR4302543.4813237/1‑94 (MQ=60)

              ACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGC                                                                                 >  SRR4302543.8008664/1‑94 (MQ=60)

              ACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGC                                                                                 >  SRR4302543.8008666/1‑94 (MQ=60)

                TGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGA                                                                               >  SRR4302543.7452338/1‑94 (MQ=60)

                  GGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGCGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACC                                                                             <  SRR4302543.5070164/94‑1 (MQ=60)

                  GGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACC                                                                             >  SRR4302543.5833946/1‑94 (MQ=60)

                     AGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTG                                                                          <  SRR4302543.3102254/94‑1 (MQ=60)

                        GTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAA                                                                       <  SRR4302543.738476/94‑1 (MQ=60)

                        GTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAA                                                                       >  SRR4302543.7604166/1‑94 (MQ=60)

                         TCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAG                                                                      <  SRR4302543.5452345/94‑1 (MQ=60)

                         TCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAG                                                                      <  SRR4302543.7491410/94‑1 (MQ=60)

                          CTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGA                                                                     >  SRR4302543.5082069/1‑94 (MQ=60)

                           CCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGAT                                                                    <  SRR4302543.1710779/94‑1 (MQ=60)

                            CTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATC                                                                   >  SRR4302543.7627966/1‑94 (MQ=60)

                             TCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGGGCGCTGGCGACCCTGAAAGATCT                                                                  >  SRR4302543.178049/1‑94 (MQ=60)

                              CCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTG                                                                 >  SRR4302543.3236856/1‑94 (MQ=60)

                              CCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTG                                                                 >  SRR4302543.5051774/1‑94 (MQ=60)

                               CAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGG                                                                <  SRR4302543.1631155/94‑1 (MQ=60)

                                AGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGC                                                               >  SRR4302543.1469318/1‑94 (MQ=60)

                                 GGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCA                                                              <  SRR4302543.7612070/94‑1 (MQ=60)

                                  GGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAG                                                             <  SRR4302543.3938405/94‑1 (MQ=60)

                                    GCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAA                                                           <  SRR4302543.5898554/94‑1 (MQ=60)

                                     GTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAA                                                          <  SRR4302543.4213348/94‑1 (MQ=60)

                                     CTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGAGACCCTGAAAGATCTGGCAGCAA                                                          >  SRR4302543.4465421/1‑94 (MQ=60)

                                      TGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAA                                                         <  SRR4302543.1739749/94‑1 (MQ=60)

                                       GGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAA                                                        >  SRR4302543.3240842/1‑94 (MQ=60)

                                       GGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAA                                                        >  SRR4302543.1347460/1‑94 (MQ=60)

                                        GAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAG                                                       <  SRR4302543.660309/94‑1 (MQ=60)

                                        GAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAG                                                       <  SRR4302543.3731022/94‑1 (MQ=60)

                                        GAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAG                                                       >  SRR4302543.7032218/1‑94 (MQ=60)

                                        GAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAG                                                       >  SRR4302543.7162838/1‑94 (MQ=60)

                                         AGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGA                                                      >  SRR4302543.1437007/1‑94 (MQ=60)

                                          GCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAG                                                     <  SRR4302543.4047315/94‑1 (MQ=60)

                                          GCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAG                                                     >  SRR4302543.2059652/1‑94 (MQ=60)

                                          GCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAG                                                     >  SRR4302543.6342885/1‑94 (MQ=60)

                                          GCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAG                                                     >  SRR4302543.6035576/1‑94 (MQ=60)

                                          GCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAG                                                     >  SRR4302543.2889649/1‑94 (MQ=60)

                                           CGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGG                                                    >  SRR4302543.5242397/1‑94 (MQ=60)

                                            GTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTTGCAGAAAAAGAGGG                                                   >  SRR4302543.2729943/1‑94 (MQ=60)

                                            GTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAaga                                                   >  SRR4302543.5544600/1‑91 (MQ=60)

                                             TATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTA                                                  >  SRR4302543.5342847/1‑94 (MQ=60)

                                             TATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTA                                                  >  SRR4302543.2931450/1‑94 (MQ=60)

                                             TATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTA                                                  <  SRR4302543.7865427/94‑1 (MQ=60)

                                              ATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAG                                                 >  SRR4302543.4004394/1‑94 (MQ=60)

                                              ATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAG                                                 <  SRR4302543.5478779/94‑1 (MQ=60)

                                              ATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAG                                                 >  SRR4302543.4297730/1‑94 (MQ=60)

                                                TTTCCCGACAGTGTCATAACCCAGGAGAGAACGGGAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAG                                               <  SRR4302543.4535157/94‑1 (MQ=60)

                                                 TTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGG                                              <  SRR4302543.7013869/94‑1 (MQ=60)

                                                 TTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGG                                              >  SRR4302543.7839121/1‑94 (MQ=60)

                                                  TCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGA                                             <  SRR4302543.5827645/94‑1 (MQ=60)

                                                   CCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGAT                                            <  SRR4302543.2977648/94‑1 (MQ=60)

                                                   CCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGAT                                            <  SRR4302543.1065366/94‑1 (MQ=60)

                                                    CCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATG                                           >  SRR4302543.3512206/1‑94 (MQ=60)

                                                     CGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGC                                          >  SRR4302543.5088627/1‑94 (MQ=60)

                                                     CGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGC                                          <  SRR4302543.1894771/94‑1 (MQ=60)

                                                     CGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGC                                          >  SRR4302543.3654941/1‑94 (MQ=60)

                                                     CGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGC                                          >  SRR4302543.1874849/1‑94 (MQ=60)

                                                      GACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCC                                         >  SRR4302543.1494640/1‑94 (MQ=60)

                                                       ACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCG                                        <  SRR4302543.2098530/94‑1 (MQ=60)

                                                       ACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCG                                        >  SRR4302543.176073/1‑94 (MQ=60)

                                                         AGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCG                                      >  SRR4302543.4667192/1‑94 (MQ=60)

                                                          GTGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGC                                     >  SRR4302543.3959227/1‑94 (MQ=60)

                                                           TGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCG                                    >  SRR4302543.5258867/1‑94 (MQ=60)

                                                           TGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCG                                    >  SRR4302543.1425003/1‑94 (MQ=60)

                                                           TGTCATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCG                                    >  SRR4302543.6869651/1‑94 (MQ=60)

                                                              CATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCT                                 >  SRR4302543.7903091/1‑94 (MQ=60)

                                                              CATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCT                                 <  SRR4302543.7907597/94‑1 (MQ=60)

                                                              CATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCT                                 <  SRR4302543.4365160/94‑1 (MQ=60)

                                                              CATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCT                                 >  SRR4302543.1288054/1‑94 (MQ=60)

                                                               ATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTG                                >  SRR4302543.4878202/1‑94 (MQ=60)

                                                               ATAACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTG                                >  SRR4302543.1862856/1‑94 (MQ=60)

                                                                  ACCCAGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTG                             <  SRR4302543.4902345/94‑1 (MQ=60)

                                                                      AGGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTG                         >  SRR4302543.241790/1‑94 (MQ=60)

                                                                       GGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGA                        >  SRR4302543.4872608/1‑94 (MQ=60)

                                                                       GGAGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGA                        <  SRR4302543.6714682/94‑1 (MQ=60)

                                                                         AGATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAA                      >  SRR4302543.5416696/1‑94 (MQ=60)

                                                                          GATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAAT                     <  SRR4302543.1135651/94‑1 (MQ=60)

                                                                          GATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAAT                     <  SRR4302543.4976939/94‑1 (MQ=60)

                                                                           ATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATG                    >  SRR4302543.2654231/1‑94 (MQ=60)

                                                                           ATAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATG                    >  SRR4302543.6592948/1‑94 (MQ=60)

                                                                            TAACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGC                   <  SRR4302543.2579272/94‑1 (MQ=60)

                                                                             AACGGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCG                  >  SRR4302543.7998135/1‑94 (MQ=60)

                                                                                GGCAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCG               >  SRR4302543.2589796/1‑94 (MQ=60)

                                                                                  CAGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCG             >  SRR4302543.5197258/1‑94 (MQ=60)

                                                                                   AGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGG            >  SRR4302543.5207420/1‑94 (MQ=60)

                                                                                   AGATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGG            >  SRR4302543.1059074/1‑94 (MQ=60)

                                                                                    GATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGA           >  SRR4302543.7638871/1‑94 (MQ=60)

                                                                                     ATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGAT          <  SRR4302543.3553414/94‑1 (MQ=60)

                                                                                     ATGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGAT          <  SRR4302543.554361/94‑1 (MQ=60)

                                                                                      TGGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATG         <  SRR4302543.2139086/94‑1 (MQ=60)

                                                                                       GGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGT        <  SRR4302543.3209941/94‑1 (MQ=60)

                                                                                       GGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGT        <  SRR4302543.4125155/94‑1 (MQ=60)

                                                                                       GGCAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGT        <  SRR4302543.4879196/94‑1 (MQ=60)

                                                                                         CAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCA      <  SRR4302543.3906964/94‑1 (MQ=60)

                                                                                         CAGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCA      <  SRR4302543.3930937/94‑1 (MQ=60)

                                                                                          AGAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAG     >  SRR4302543.5426217/1‑94 (MQ=60)

                                                                                           GAGCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAGC    <  SRR4302543.3047437/94‑1 (MQ=60)

                                                                                             GCATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAGCAG  <  SRR4302543.5516718/94‑1 (MQ=60)

TTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAGCAG  >  NC_000913/2015883‑2016069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`