Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,017,469 G→A T354T (ACG→ACA)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,017,4690GA99.2% 811.7 / ‑6.1 240T354T (ACG→ACA) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (128/108);  total (129/111)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 247 total aligned reads displayed.

CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558
                                                                                          |                                                                                         
cTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACt                                                                                         >  1:7649760/1‑93 (MQ=255)
 tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGCAGACGACGATACACtt                                                                                        >  1:5233597/1‑93 (MQ=255)
  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       <  2:5792646/93‑1 (MQ=255)
  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       >  1:946885/1‑93 (MQ=255)
  ttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTc                                                                                       <  1:2867154/93‑1 (MQ=255)
   tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTcc                                                                                      <  2:1603018/93‑1 (MQ=255)
    aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCg                                                                                     >  2:1647105/1‑93 (MQ=255)
       ggTCAGCAGCAGGCCGCTGCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGtt                                                                                  <  2:6890151/93‑1 (MQ=255)
         tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTcc                                                                                >  1:2304077/1‑93 (MQ=255)
         tCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTcc                                                                                >  1:3321691/1‑93 (MQ=255)
            gcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGt                                                                             >  2:4612826/1‑93 (MQ=255)
               gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGtctc                                                                          >  1:4870640/1‑93 (MQ=255)
                 agGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCtt                                                                        >  2:377447/1‑93 (MQ=255)
                   gCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTa                                                                      <  2:5839071/93‑1 (MQ=255)
                     cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACa                                                                    >  2:4255883/1‑93 (MQ=255)
                     cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAAACTCTGGCGGGGGAGGACGAAGATACACTTCCGGTTCCCCTCTCTTTTCa                                                                    >  2:4951695/1‑93 (MQ=255)
                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   <  2:1242675/93‑1 (MQ=255)
                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   <  2:6637348/93‑1 (MQ=255)
                      gCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGAGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACaa                                                                   >  2:3573906/1‑93 (MQ=255)
                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAg                                                                  <  1:7494877/93‑1 (MQ=255)
                       cTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGCTTCTCGTCTCTTTAAAAg                                                                  >  2:7582003/1‑93 (MQ=255)
                        ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTACGGTTCCCGTCTCTTTACAAgg                                                                 >  2:7349016/1‑93 (MQ=255)
                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                                <  1:727485/93‑1 (MQ=255)
                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                                <  2:4720737/93‑1 (MQ=255)
                         tCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAggg                                                                >  1:6938058/1‑93 (MQ=255)
                           ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCg                                                              >  1:3928052/1‑93 (MQ=255)
                           ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCg                                                              <  1:5572783/93‑1 (MQ=255)
                           ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCg                                                              >  2:1819858/1‑93 (MQ=255)
                            cagcagaagcaaAGCCATGGCACAGCAAACCATGAACCTCTGGAGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgt                                                             <  2:86714/93‑1 (MQ=255)
                            cagcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgt                                                             >  1:3546090/1‑93 (MQ=255)
                            cagcaaaagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgt                                                             <  2:5972508/93‑1 (MQ=255)
                             agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                            >  2:253268/1‑93 (MQ=255)
                             agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCgtg                                                            >  2:2130816/1‑93 (MQ=255)
                               caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTa                                                          <  2:3002866/93‑1 (MQ=255)
                               caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTa                                                          <  2:5225962/93‑1 (MQ=255)
                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGTGCGTGTaa                                                         >  2:7269109/1‑93 (MQ=255)
                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         <  2:5703941/93‑1 (MQ=255)
                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         >  2:7678439/1‑93 (MQ=255)
                                aacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTaa                                                         <  2:5172264/93‑1 (MQ=255)
                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACCCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        >  2:6107503/1‑93 (MQ=255)
                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        >  2:618774/1‑93 (MQ=255)
                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        <  1:3222113/93‑1 (MQ=255)
                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        <  2:1760246/93‑1 (MQ=255)
                                 acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAc                                                        >  2:5350812/1‑93 (MQ=255)
                                  cagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACa                                                       <  2:7201577/93‑1 (MQ=255)
                                   agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAg                                                      <  2:2300095/93‑1 (MQ=255)
                                   agcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAg                                                      >  1:4654853/1‑93 (MQ=255)
                                      aaaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCa                                                   <  2:692611/93‑1 (MQ=255)
                                       aaGCCACCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  <  2:7836302/93‑1 (MQ=255)
                                       aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCaa                                                  >  1:4264606/1‑93 (MQ=255)
                                        aGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAAc                                                 <  2:5687387/93‑1 (MQ=255)
                                         gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACa                                                >  2:2011778/1‑93 (MQ=255)
                                          ccAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTTTTTACAAGGGCGTGTAACAGGCAACAg                                               >  2:222908/1‑93 (MQ=255)
                                           cAACGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  2:1300617/93‑1 (MQ=255)
                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              >  1:1948873/1‑93 (MQ=255)
                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  2:1438951/93‑1 (MQ=255)
                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              >  1:3739280/1‑93 (MQ=255)
                                           cAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgc                                              <  2:6586588/93‑1 (MQ=255)
                                             aCGAACAGCAAACCATGAACCTCTGGCTGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcgg                                            <  2:1612635/93‑1 (MQ=255)
                                              cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggc                                           <  1:2793593/93‑1 (MQ=255)
                                              cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAgcggc                                           >  1:7433638/1‑93 (MQ=255)
                                                 acaGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                        >  1:4301816/1‑93 (MQ=255)
                                                   aGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGa                                      <  1:1233985/93‑1 (MQ=255)
                                                    gCAACCCATGAACCTCT‑GCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                    <  2:709914/93‑1 (MQ=255)
                                                    gCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                     <  1:5114051/93‑1 (MQ=255)
                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCGCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  2:4677881/1‑93 (MQ=255)
                                                       aaCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                  >  1:7357803/1‑93 (MQ=255)
                                                        aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                 >  2:6990466/1‑93 (MQ=255)
                                                        aCCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAttt                                 >  1:3484740/1‑93 (MQ=255)
                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               <  1:7707043/93‑1 (MQ=255)
                                                          cATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTc                               <  1:841475/93‑1 (MQ=255)
                                                           aTGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCg                              >  1:4339881/1‑93 (MQ=255)
                                                            tGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                             >  1:7812401/1‑93 (MQ=255)
                                                             gAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                            >  2:2617368/1‑93 (MQ=255)
                                                               cccTCTGCCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTa                          >  2:4798415/2‑93 (MQ=255)
                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        <  1:889147/93‑1 (MQ=255)
                                                                 ctctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAc                        >  1:63214/1‑93 (MQ=255)
                                                                  tctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTacat                       >  1:5123745/1‑90 (MQ=255)
                                                                  tctGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACg                       <  1:6968589/93‑1 (MQ=255)
                                                                    cggCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                     <  1:6211802/92‑1 (MQ=255)
                                                                     ggCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                    <  2:5582474/93‑1 (MQ=255)
                                                                      gCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                   >  1:4457186/1‑93 (MQ=255)
                                                                       cGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                  <  2:7460519/93‑1 (MQ=255)
                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATCTTTTCGCCTAACGTCAGAGGt               <  1:1409558/93‑1 (MQ=255)
                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt               >  2:2875756/1‑93 (MQ=255)
                                                                          gggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGt               >  2:4499915/1‑93 (MQ=255)
                                                                           ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa              >  1:5277271/1‑93 (MQ=255)
                                                                           ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa              <  1:2546084/93‑1 (MQ=255)
                                                                           ggAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTa              >  1:1314975/1‑93 (MQ=255)
                                                                               gacgacGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAc          >  1:1994570/1‑93 (MQ=255)
                                                                                  gacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt       >  1:3680682/1‑93 (MQ=255)
                                                                                  gacgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGt       <  1:2542010/93‑1 (MQ=255)
                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa      >  1:2462540/1‑93 (MQ=255)
                                                                                   acgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa      >  2:3253134/1‑93 (MQ=255)
                                                                                    cgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa     <  1:5383150/93‑1 (MQ=255)
                                                                                    cgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa     >  1:7091062/1‑93 (MQ=255)
                                                                                    cgaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTaa     >  2:3260488/1‑93 (MQ=255)
                                                                                     gaTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTatc    >  2:3622800/1‑91 (MQ=255)
                                                                                      aTACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc   >  2:4073803/1‑93 (MQ=255)
                                                                                       tACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc  <  2:1021219/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC  >  NC_000913/2017379‑2017558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  >  NC_000913/2017376‑2017562
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AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCCCTGGCGGGGGAAGACGACGATACA                                                                                               <  SRR4302543.4804780/94‑1 (MQ=60)
  gatttgtTGGTCAGCAGCAGGCCGCGTCCCAGCAACATCAAAGCCAACGCCCAGCATATCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                             <  SRR4302543.641694/87‑1 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCACCAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                             <  SRR4302543.721524/94‑1 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                             <  SRR4302543.641696/94‑1 (MQ=60)
  GCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACT                                                                                             >  SRR4302543.7722296/1‑94 (MQ=60)
   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGACCCTCTGGCGGGGGAAGACGACGATACACTT                                                                                            <  SRR4302543.5166853/94‑1 (MQ=60)
   CTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGCAGACGACGATACACTT                                                                                            >  SRR4302543.5280750/1‑94 (MQ=60)
    TTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTC                                                                                           >  SRR4302543.955158/1‑94 (MQ=60)
     TTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCC                                                                                          <  SRR4302543.2893281/94‑1 (MQ=60)
         TGGTCAGCAGCAGTCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGGAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTT                                                                                      <  SRR4302543.4307464/94‑1 (MQ=60)
           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                    >  SRR4302543.3351657/1‑94 (MQ=60)
           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                    <  SRR4302543.4347921/94‑1 (MQ=60)
           GTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCC                                                                                    >  SRR4302543.2325065/1‑94 (MQ=60)
            TCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCC                                                                                   >  SRR4302543.4719470/1‑94 (MQ=60)
             CAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCG                                                                                  <  SRR4302543.7444106/94‑1 (MQ=60)
                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                               >  SRR4302543.4431373/1‑94 (MQ=60)
                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                               <  SRR4302543.7920926/94‑1 (MQ=60)
                CAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCT                                                                               >  SRR4302543.6362479/1‑94 (MQ=60)
                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGAGGACGATACACTTCCGGTTCCCGTCTC                                                                              >  SRR4302543.2533228/1‑94 (MQ=60)
                 AGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTC                                                                              >  SRR4302543.4915040/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                           >  SRR4302543.4051118/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                           >  SRR4302543.4685789/1‑94 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                           <  SRR4302543.357075/94‑1 (MQ=60)
                    AGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTT                                                                           >  SRR4302543.5852648/1‑94 (MQ=60)
                     GGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTA                                                                          >  SRR4302543.7382264/1‑94 (MQ=60)
                       CCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACA                                                                        >  SRR4302543.6739089/1‑94 (MQ=60)
                         GCTTCCCAGCAGCAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                      <  SRR4302543.3891458/94‑1 (MQ=60)
                         GCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAG                                                                      <  SRR4302543.3345492/94‑1 (MQ=60)
                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                     >  SRR4302543.3760957/1‑94 (MQ=60)
                          CTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGG                                                                     <  SRR4302543.7566572/94‑1 (MQ=60)
                           TTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGG                                                                    >  SRR4302543.7003322/1‑94 (MQ=60)
                            TCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGC                                                                   <  SRR4302543.734024/94‑1 (MQ=60)
                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                  >  SRR4302543.4999366/1‑94 (MQ=60)
                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                  <  SRR4302543.2646258/94‑1 (MQ=60)
                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                  <  SRR4302543.7614883/94‑1 (MQ=60)
                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                  >  SRR4302543.1824983/1‑94 (MQ=60)
                             CCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCG                                                                  >  SRR4302543.3962721/1‑94 (MQ=60)
                              CCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGT                                                                 <  SRR4302543.5623502/94‑1 (MQ=60)
                              CCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGT                                                                 >  SRR4302543.3577574/1‑94 (MQ=60)
                               CAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTG                                                                <  SRR4302543.3368550/94‑1 (MQ=60)
                                AGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGT                                                               >  SRR4302543.4599900/1‑94 (MQ=60)
                                   AACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACCAGGGCGTGTAAC                                                            <  SRR4302543.1400655/94‑1 (MQ=60)
                                   AACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAAC                                                            <  SRR4302543.1321863/94‑1 (MQ=60)
                                    ACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACA                                                           <  SRR4302543.5252420/94‑1 (MQ=60)
                                    ACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACA                                                           <  SRR4302543.3251233/94‑1 (MQ=60)
                                    ACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACA                                                           >  SRR4302543.2681126/1‑94 (MQ=60)
                                     CAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAG                                                          >  SRR4302543.4697241/1‑94 (MQ=60)
                                      AGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGG                                                         >  SRR4302543.2431083/1‑94 (MQ=60)
                                        CAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCA                                                       <  SRR4302543.6926611/94‑1 (MQ=60)
                                         AAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAA                                                      >  SRR4302543.4303339/1‑94 (MQ=60)
                                           AGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACA                                                    <  SRR4302543.3420275/94‑1 (MQ=60)
                                            GCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAG                                                   <  SRR4302543.5134905/94‑1 (MQ=60)
                                             CCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                  >  SRR4302543.1966357/1‑94 (MQ=60)
                                             CCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGC                                                  >  SRR4302543.3772173/1‑94 (MQ=60)
                                               AACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGG                                                >  SRR4302543.1974890/1‑94 (MQ=60)
                                                ACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGC                                               >  SRR4302543.7504899/1‑94 (MQ=60)
                                                ACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGC                                               >  SRR4302543.7504900/1‑94 (MQ=60)
                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                              <  SRR4302543.2819116/94‑1 (MQ=60)
                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                              >  SRR4302543.7673637/1‑94 (MQ=60)
                                                 CGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCG                                              <  SRR4302543.5266657/94‑1 (MQ=60)
                                                   CACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTT                                            <  SRR4302543.5338097/94‑1 (MQ=60)
                                                   CACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTT                                            >  SRR4302543.4340900/1‑94 (MQ=60)
                                                   CACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTT                                            >  SRR4302543.938728/1‑94 (MQ=60)
                                                     CAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGA                                          <  SRR4302543.5309979/94‑1 (MQ=60)
                                                      AGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGAT                                         <  SRR4302543.1245099/94‑1 (MQ=60)
                                                       GCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATA                                        <  SRR4302543.5160313/94‑1 (MQ=60)
                                                        CAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAT                                       <  SRR4302543.7437655/94‑1 (MQ=60)
                                                        CAAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAT                                       <  SRR4302543.5380162/94‑1 (MQ=60)
                                                         AAACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATT                                      >  SRR4302543.7428570/1‑94 (MQ=60)
                                                          AACCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTT                                     >  SRR4302543.3515948/1‑94 (MQ=60)
                                                            CCATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTC                                   >  SRR4302543.2544212/1‑94 (MQ=60)
                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                  <  SRR4302543.7779954/94‑1 (MQ=60)
                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                  <  SRR4302543.848912/94‑1 (MQ=60)
                                                             CATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCG                                  >  SRR4302543.4379250/1‑94 (MQ=60)
                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                 >  SRR4302543.7886030/1‑94 (MQ=60)
                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                 >  SRR4302543.7829029/1‑94 (MQ=60)
                                                              ATGAACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGC                                 >  SRR4302543.2566184/1‑94 (MQ=60)
                                                                 AACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTA                              <  SRR4302543.3113696/94‑1 (MQ=60)
                                                                  ACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA                             >  SRR4302543.777127/1‑94 (MQ=60)
                                                                  ACCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAA                             >  SRR4302543.872041/1‑94 (MQ=60)
                                                                   CCTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAAC                            >  SRR4302543.63769/1‑94 (MQ=60)
                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAcat                           >  SRR4302543.5170071/1‑91 (MQ=60)
                                                                    CTCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACG                           <  SRR4302543.897015/94‑1 (MQ=60)
                                                                     TCTGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGT                          <  SRR4302543.7034164/94‑1 (MQ=60)
                                                                       CGGCGGGGGAAGACGACGATACCCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCA                        <  SRR4302543.957635/94‑1 (MQ=60)
                                                                       CGGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCA                        <  SRR4302543.6270456/94‑1 (MQ=60)
                                                                        GGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                       >  SRR4302543.4497580/1‑94 (MQ=60)
                                                                        GGCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAG                       <  SRR4302543.2865842/94‑1 (MQ=60)
                                                                         GCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGA                      >  SRR4302543.7791765/1‑94 (MQ=60)
                                                                         GCGGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGA                      <  SRR4302543.3624292/94‑1 (MQ=60)
                                                                          CGGGGGAAGACGACGATACACTTCCGGTGCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAG                     <  SRR4302543.714612/94‑1 (MQ=60)
                                                                           GGGGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGG                    <  SRR4302543.88317/94‑1 (MQ=60)
                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATCTTTTCGCCTAACGTCAGAGGTA                  <  SRR4302543.1422295/94‑1 (MQ=60)
                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                  >  SRR4302543.1326658/1‑94 (MQ=60)
                                                                             GGGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTA                  >  SRR4302543.5324882/1‑94 (MQ=60)
                                                                              GGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                 <  SRR4302543.2028949/94‑1 (MQ=60)
                                                                              GGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                 <  SRR4302543.7021675/94‑1 (MQ=60)
                                                                              GGAAGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                 <  SRR4302543.2569308/94‑1 (MQ=60)
                                                                              GGAAGACGACGATACACTTCCGGTTCCCATCTCTGTACAAGGGCTTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAG                 <  SRR4302543.2028948/94‑1 (MQ=60)
                                                                                 AGACGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAC              >  SRR4302543.2012457/1‑94 (MQ=60)
                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGT           >  SRR4302543.3713023/1‑94 (MQ=60)
                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGT           >  SRR4302543.16389/1‑94 (MQ=60)
                                                                                    CGACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGGAGCACCGT           >  SRR4302543.3037424/1‑94 (MQ=60)
                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA          >  SRR4302543.2484745/1‑94 (MQ=60)
                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA          >  SRR4302543.6050398/1‑94 (MQ=60)
                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA          >  SRR4302543.7289180/1‑94 (MQ=60)
                                                                                     GACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTA          <  SRR4302543.2565187/94‑1 (MQ=60)
                                                                                      ACGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAA         >  SRR4302543.7159929/1‑94 (MQ=60)
                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT        >  SRR4302543.414583/1‑94 (MQ=60)
                                                                                       CGATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAT        <  SRR4302543.5432015/94‑1 (MQ=60)
                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC       >  SRR4302543.7248895/1‑94 (MQ=60)
                                                                                        GATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATC       >  SRR4302543.4356968/1‑94 (MQ=60)
                                                                                         ATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACGGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC      >  SRR4302543.5563660/1‑94 (MQ=60)
                                                                                         ATACACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCC      >  SRR4302543.3693905/1‑94 (MQ=60)
                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG   <  SRR4302543.3782588/94‑1 (MQ=60)
                                                                                            CACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCG   >  SRR4302543.1159109/1‑94 (MQ=60)
                                                                                             ACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  >  SRR4302543.6879428/1‑94 (MQ=60)
                                                                                             ACTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  >  SRR4302543.6879427/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGT  >  NC_000913/2017376‑2017562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑