Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,063,343 T→C A148A (GCA→GCG)  cobU ← cobinamide kinase/cobinamide phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,063,3430TC99.5% 613.5 / ‑4.9 198A148A (GCA→GCG) cobUcobinamide kinase/cobinamide phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (94/103);  total (94/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

Only 100 of 204 total aligned reads displayed.

ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA  >  NC_000913/2063254‑2063432
                                                                                         |                                                                                         
aCTCCAATCCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGcc                                                                                        <  1:3206630/93‑1 (MQ=255)
 cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                       >  2:4789057/1‑93 (MQ=255)
 cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                       >  1:3428120/1‑93 (MQ=255)
 cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                       <  2:2660043/93‑1 (MQ=255)
 cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                       >  1:5360242/1‑93 (MQ=255)
   ccAATACCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                     <  2:4664843/93‑1 (MQ=255)
    cAATACCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgac                                                                                    <  1:5859973/93‑1 (MQ=255)
     aaTCCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacg                                                                                   <  1:6421695/93‑1 (MQ=255)
     aaTACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacg                                                                                   >  1:2399303/1‑93 (MQ=255)
     aaTACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacg                                                                                   >  1:2556952/1‑93 (MQ=255)
      aTACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacga                                                                                  >  1:1901874/1‑93 (MQ=255)
       tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 <  2:6836333/93‑1 (MQ=255)
       tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 >  2:3937805/1‑93 (MQ=255)
       tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 >  2:5389515/1‑93 (MQ=255)
       tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 >  1:4256294/1‑93 (MQ=255)
       tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCACCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 >  1:2223970/1‑93 (MQ=255)
         cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               >  1:129148/1‑93 (MQ=255)
          ccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGAGCCAgccgaacct                                                                              >  1:4053842/1‑89 (MQ=255)
           cGAACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             <  2:930240/93‑1 (MQ=255)
           cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             <  2:3522811/93‑1 (MQ=255)
           cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             <  2:2179022/93‑1 (MQ=255)
            gAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTcc                                                                            <  1:4354315/93‑1 (MQ=255)
            gAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTcc                                                                            >  2:3229055/1‑93 (MQ=255)
            gAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTcc                                                                            <  1:3529138/93‑1 (MQ=255)
             aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           <  1:3794907/93‑1 (MQ=255)
              aaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCgc                                                                          >  1:1002593/1‑92 (MQ=255)
               accaccAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGc                                                                         >  2:4586627/1‑93 (MQ=255)
                  accaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcaca                                                                      >  2:3651450/1‑93 (MQ=255)
                    caGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAAt                                                                    <  1:7491981/93‑1 (MQ=255)
                      gCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcc                                                                  >  2:7083595/1‑93 (MQ=255)
                      gCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcc                                                                  >  1:1999263/1‑93 (MQ=255)
                       ccATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATccc                                                                 <  2:2517347/93‑1 (MQ=255)
                         aTACTTCATTTGCCGCAGCGGCCAACTGCTTATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCa                                                               <  2:5162513/93‑1 (MQ=255)
                         aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               <  1:6349546/93‑1 (MQ=255)
                          tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  2:2342397/1‑93 (MQ=255)
                          tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:3798867/1‑93 (MQ=255)
                          tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:7808539/1‑93 (MQ=255)
                          tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:6031244/1‑93 (MQ=255)
                            cTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTc                                                            >  1:6307565/1‑93 (MQ=255)
                              tCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTccc                                                          <  2:4362870/93‑1 (MQ=255)
                               cATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCa                                                         >  1:249172/1‑93 (MQ=255)
                               cATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCa                                                         <  1:1585650/93‑1 (MQ=255)
                               cATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCa                                                         <  1:6359318/93‑1 (MQ=255)
                                aTTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAc                                                        >  1:4705545/1‑93 (MQ=255)
                                aTTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAc                                                        >  2:5768057/1‑93 (MQ=255)
                                   tGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGACGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTc                                                     >  2:7866088/1‑93 (MQ=255)
                                    gCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCg                                                    >  2:6545637/1‑93 (MQ=255)
                                      cGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGtt                                                  >  2:4163321/1‑93 (MQ=255)
                                       gCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTa                                                 <  1:7307966/93‑1 (MQ=255)
                                       gCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTa                                                 <  1:6486373/93‑1 (MQ=255)
                                          gCGGCCAACTGCTGATTTACCCGCCTGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTc                                              >  1:6441206/1‑93 (MQ=255)
                                          gCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTc                                              >  1:1089560/1‑93 (MQ=255)
                                          gCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTc                                              >  1:2287408/1‑93 (MQ=255)
                                            ggCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAc                                            <  1:1173140/93‑1 (MQ=255)
                                             gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           >  2:2771259/1‑93 (MQ=255)
                                               cAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTaa                                         >  2:1251468/1‑93 (MQ=255)
                                               cAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTaa                                         >  2:2086501/1‑93 (MQ=255)
                                                aaCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAAt                                        <  2:6434255/93‑1 (MQ=255)
                                                 aCTGCTGATTTGCCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGGCTCTCCGGCACAATCACCATTCCCACTTCGTTAGTCACTAATa                                       <  2:498341/93‑1 (MQ=255)
                                                 aCTGCTGATTTCCCCGCCCGGCAATATCACGAATATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       <  1:7623113/93‑1 (MQ=255)
                                                 aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCGCTAATa                                       <  2:6303896/93‑1 (MQ=255)
                                                 aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       <  1:1894674/93‑1 (MQ=255)
                                                 aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       >  1:5841566/1‑93 (MQ=255)
                                                  ctgctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAc                                      <  2:3414698/93‑1 (MQ=255)
                                                   tgctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACa                                     <  2:2990855/93‑1 (MQ=255)
                                                   tgctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACa                                     >  1:301107/1‑93 (MQ=255)
                                                         attACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCttt                               <  2:6401027/92‑1 (MQ=255)
                                                          ttACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTg                              <  1:5913692/93‑1 (MQ=255)
                                                             cccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCgg                           >  1:3100903/1‑93 (MQ=255)
                                                             cccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCgg                           <  1:4583786/93‑1 (MQ=255)
                                                             cccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCgg                           <  1:2297523/93‑1 (MQ=255)
                                                              ccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCggg                          >  1:4150536/1‑93 (MQ=255)
                                                              ccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCggg                          >  1:3501113/1‑93 (MQ=255)
                                                               cgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCgggg                         <  1:6814150/93‑1 (MQ=255)
                                                                gcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGc                        >  2:1313091/1‑93 (MQ=255)
                                                                 cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                       >  2:5485492/1‑93 (MQ=255)
                                                                  ccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCaa                      >  1:512106/1‑93 (MQ=255)
                                                                    ggCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACg                    >  2:3980272/1‑93 (MQ=255)
                                                                    ggCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACg                    >  2:4929281/1‑93 (MQ=255)
                                                                      cAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGtt                  <  1:3908530/93‑1 (MQ=255)
                                                                       aaTATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTg                 <  2:5247862/93‑1 (MQ=255)
                                                                       aaTATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTg                 >  2:5886364/1‑93 (MQ=255)
                                                                        atatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTgg                <  1:3139933/93‑1 (MQ=255)
                                                                          atCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggca              <  1:5765349/93‑1 (MQ=255)
                                                                             aCGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcaggc           <  1:5020625/93‑1 (MQ=255)
                                                                             aCGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcaggc           <  2:3456912/93‑1 (MQ=255)
                                                                              cGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcaggca          >  1:5954910/1‑93 (MQ=255)
                                                                                aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        >  1:330752/1‑93 (MQ=255)
                                                                                aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        >  1:5693160/1‑93 (MQ=255)
                                                                                aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        <  1:2874237/93‑1 (MQ=255)
                                                                                  aaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCaa      >  2:7712432/1‑93 (MQ=255)
                                                                                    tGTCGCGCCAGACGCCTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATc    <  2:7452350/93‑1 (MQ=255)
                                                                                    tGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATc    <  2:187656/93‑1 (MQ=255)
                                                                                    tGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTCTGCGGGGCAACGTTGGCAGGCAGCAATc    <  2:2394500/93‑1 (MQ=255)
                                                                                     gTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCa   >  1:5234143/1‑93 (MQ=255)
                                                                                     gTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCa   <  2:370895/93‑1 (MQ=255)
                                                                                      tCGCGCCAGACGCCTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCaa  <  1:889958/93‑1 (MQ=255)
                                                                                      tCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCaa  >  2:6242145/1‑93 (MQ=255)
                                                                                      tCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCaa  <  2:6202814/93‑1 (MQ=255)
                                                                                      tCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCaa  <  2:3119330/93‑1 (MQ=255)
                                                                                         |                                                                                         
ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA  >  NC_000913/2063254‑2063432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG  >  NC_000913/2063250‑2063434
                                                                                             |                                                                                           
TTTTACTCCAATACCCGAACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGC                                                                                             <  SRR4302543.4924438/94‑1 (MQ=60)
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGC                                                                                             >  SRR4302543.1523046/1‑94 (MQ=60)
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGC                                                                                             >  SRR4302543.4909353/1‑94 (MQ=60)
   TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCC                                                                                          >  SRR4302543.2551206/1‑94 (MQ=60)
    ACTCCAATCCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCA                                                                                         <  SRR4302543.3235564/94‑1 (MQ=60)
    ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCA                                                                                         >  SRR4302543.5408751/1‑94 (MQ=60)
    ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCA                                                                                         >  SRR4302543.3458796/1‑94 (MQ=60)
        CAATACCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                     <  SRR4302543.5913308/94‑1 (MQ=60)
        CAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                     >  SRR4302543.2421002/1‑94 (MQ=60)
        CAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                     >  SRR4302543.2580328/1‑94 (MQ=60)
        CAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCCCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                     <  SRR4302543.2416143/94‑1 (MQ=60)
         AATCCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGA                                                                                    <  SRR4302543.6482202/94‑1 (MQ=60)
         AATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGA                                                                                    >  SRR4302543.1918986/1‑94 (MQ=60)
          ATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAC                                                                                   <  SRR4302543.793215/94‑1 (MQ=60)
          ATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAC                                                                                   >  SRR4302543.4294935/1‑94 (MQ=60)
          ATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCACGCCAAACGAC                                                                                   >  SRR4302543.6519360/1‑94 (MQ=60)
          ATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCACCCGGCAATATCACGAAAATGTCGCGCCAGACGAC                                                                                   >  SRR4302543.2244046/1‑94 (MQ=60)
           TCCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACT                                                                                  <  SRR4302543.4924156/94‑1 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                 >  SRR4302543.130304/1‑94 (MQ=60)
            ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                 >  SRR4302543.130303/1‑94 (MQ=60)
             CCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCT                                                                                <  SRR4302543.1087127/94‑1 (MQ=60)
             CCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCT                                                                                <  SRR4302543.5981365/94‑1 (MQ=60)
             CCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCT                                                                                >  SRR4302543.4583604/1‑94 (MQ=60)
             CCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGAGCCAGccgaacct                                                                                >  SRR4302543.4090121/1‑86 (MQ=60)
              CCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTC                                                                               >  SRR4302543.5932730/1‑94 (MQ=60)
               CGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCC                                                                              <  SRR4302543.2869709/94‑1 (MQ=60)
                GAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCG                                                                             <  SRR4302543.4393776/94‑1 (MQ=60)
                GAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCG                                                                             <  SRR4302543.3560580/94‑1 (MQ=60)
                GAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCG                                                                             <  SRR4302543.6345300/94‑1 (MQ=60)
                 AAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGG                                                                            <  SRR4302543.3828340/94‑1 (MQ=60)
                 AAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGC                                                                            >  SRR4302543.1011454/1‑94 (MQ=60)
                    CCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                         >  SRR4302543.351965/1‑94 (MQ=60)
                        CAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATC                                                                     <  SRR4302543.7563658/94‑1 (MQ=60)
                        CAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATC                                                                     <  SRR4302543.7563657/94‑1 (MQ=60)
                         AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                    >  SRR4302543.6034624/1‑94 (MQ=60)
                         AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                    >  SRR4302543.2017207/1‑94 (MQ=60)
                         AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                    <  SRR4302543.5802867/94‑1 (MQ=60)
                           CCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCC                                                                  >  SRR4302543.415471/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                <  SRR4302543.4349423/94‑1 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                >  SRR4302543.3832336/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                >  SRR4302543.7882140/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                >  SRR4302543.6087709/1‑94 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                <  SRR4302543.6409251/94‑1 (MQ=60)
                             ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                >  SRR4302543.2488943/1‑94 (MQ=60)
                              TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                               <  SRR4302543.5896259/94‑1 (MQ=60)
                               TCTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTC                                                              >  SRR4302543.12097/1‑94 (MQ=60)
                               ACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTC                                                              >  SRR4302543.6366889/1‑94 (MQ=60)
                                  TCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCA                                                           >  SRR4302543.251337/1‑94 (MQ=60)
                                   CATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAC                                                          <  SRR4302543.1599807/94‑1 (MQ=60)
                                   CATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAC                                                          <  SRR4302543.6419113/94‑1 (MQ=60)
                                   CATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAC                                                          >  SRR4302543.4748540/1‑94 (MQ=60)
                                   CATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAC                                                          <  SRR4302543.3061029/94‑1 (MQ=60)
                                   CATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAC                                                          <  SRR4302543.6419112/94‑1 (MQ=60)
                                      TTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTC                                                       <  SRR4302543.4035077/94‑1 (MQ=60)
                                       TGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                      <  SRR4302543.649614/94‑1 (MQ=60)
                                        GCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGT                                                     >  SRR4302543.3555607/1‑94 (MQ=60)
                                           GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                  <  SRR4302543.5495835/94‑1 (MQ=60)
                                           GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                  <  SRR4302543.384143/94‑1 (MQ=60)
                                           GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                  <  SRR4302543.6547486/94‑1 (MQ=60)
                                           GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                  <  SRR4302543.7378428/94‑1 (MQ=60)
                                             AGCGGCCAACTGCTGATTTACCCGCCTGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTC                                                >  SRR4302543.6501888/1‑94 (MQ=60)
                                             AGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTC                                                >  SRR4302543.2308294/1‑94 (MQ=60)
                                             AGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTC                                                >  SRR4302543.1099351/1‑94 (MQ=60)
                                                GGCCAACTGCTGATTTACCCGCCCGTCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACT                                             <  SRR4302543.6393377/94‑1 (MQ=60)
                                                GGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACT                                             <  SRR4302543.1183777/94‑1 (MQ=60)
                                                    AACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATA                                         >  SRR4302543.5894710/1‑94 (MQ=60)
                                                    AACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATA                                         >  SRR4302543.3172273/1‑94 (MQ=60)
                                                     TCTGCTGATTTCCCCGCCCGGCCATATCACGAAAGTGTCGCGCCAGACGACTCTCCGGCCCAATCCCCATTCCCACTTCGTTAGTCCCTAATAC                                        <  SRR4302543.7101943/94‑1 (MQ=60)
                                                     ACTGCTGATTTCCCCGCCCGGCAATATCACGAATATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                        <  SRR4302543.7695458/94‑1 (MQ=60)
                                                     ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                        <  SRR4302543.1911711/94‑1 (MQ=60)
                                                     ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                        <  SRR4302543.1911712/94‑1 (MQ=60)
                                                      CTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACA                                       <  SRR4302543.3281167/94‑1 (MQ=60)
                                                      CTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACA                                       >  SRR4302543.303813/1‑94 (MQ=60)
                                                       TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                      >  SRR4302543.1927931/1‑94 (MQ=60)
                                                           GATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTT                                  <  SRR4302543.3867947/94‑1 (MQ=60)
                                                             TTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTG                                <  SRR4302543.6800518/94‑1 (MQ=60)
                                                              TTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGC                               <  SRR4302543.641396/94‑1 (MQ=60)
                                                              TTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGC                               <  SRR4302543.5967695/94‑1 (MQ=60)
                                                                ACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGG                             >  SRR4302543.2975662/1‑94 (MQ=60)
                                                                ACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGG                             >  SRR4302543.3128796/1‑94 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                            <  SRR4302543.2318474/94‑1 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                            <  SRR4302543.2318475/94‑1 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                            <  SRR4302543.4625437/94‑1 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                            >  SRR4302543.3532423/1‑94 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                            >  SRR4302543.1103453/1‑94 (MQ=60)
                                                                 CCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGG                            >  SRR4302543.4188059/1‑94 (MQ=60)
                                                                   CGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGC                          <  SRR4302543.6878481/94‑1 (MQ=60)
                                                                     CCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAA                        <  SRR4302543.422700/94‑1 (MQ=60)
                                                                     CCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAA                        >  SRR4302543.516870/1‑94 (MQ=60)
                                                                      CCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAC                       <  SRR4302543.1753063/94‑1 (MQ=60)
                                                                       CGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACG                      <  SRR4302543.5364345/94‑1 (MQ=60)
                                                                          CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                   <  SRR4302543.2074218/94‑1 (MQ=60)
                                                                          CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                   <  SRR4302543.7689178/94‑1 (MQ=60)
                                                                          CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                   >  SRR4302543.7351678/1‑94 (MQ=60)
                                                                          CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                   <  SRR4302543.3943033/94‑1 (MQ=60)
                                                                           AATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGG                  >  SRR4302543.3829035/1‑94 (MQ=60)
                                                                           AATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGG                  >  SRR4302543.3383241/1‑94 (MQ=60)
                                                                            ATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGC                 <  SRR4302543.7780685/94‑1 (MQ=60)
                                                                            ATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGC                 <  SRR4302543.3168175/94‑1 (MQ=60)
                                                                             TATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCA                >  SRR4302543.5255699/1‑94 (MQ=60)
                                                                              ATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAG               <  SRR4302543.5821009/94‑1 (MQ=60)
                                                                              ATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAG               <  SRR4302543.5817861/94‑1 (MQ=60)
                                                                               TCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGG              <  SRR4302543.5116995/94‑1 (MQ=60)
                                                                                 ACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCA            >  SRR4302543.6009411/1‑94 (MQ=60)
                                                                                 ACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCA            <  SRR4302543.5066317/94‑1 (MQ=60)
                                                                                  CGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAG           >  SRR4302543.2296951/1‑94 (MQ=60)
                                                                                   GAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGC          >  SRR4302543.3817022/1‑94 (MQ=60)
                                                                                   GAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGC          >  SRR4302543.333780/1‑94 (MQ=60)
                                                                                   GAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGC          >  SRR4302543.3136641/1‑94 (MQ=60)
                                                                                   GAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGC          >  SRR4302543.5745108/1‑94 (MQ=60)
                                                                                    AAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCA         <  SRR4302543.2900401/94‑1 (MQ=60)
                                                                                        TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA     >  SRR4302543.5281304/1‑94 (MQ=60)
                                                                                         GTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA    >  SRR4302543.4716134/1‑94 (MQ=60)
                                                                                          TCGCGCCAGACGCCTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAAC   <  SRR4302543.897831/94‑1 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG  <  SRR4302543.3628504/94‑1 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG  <  SRR4302543.4424043/94‑1 (MQ=60)
                                                                                           CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG  <  SRR4302543.4588390/94‑1 (MQ=60)
                                                                                             |                                                                                           
TTTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG  >  NC_000913/2063250‑2063434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑