Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,065,866 T→C intergenic (‑523/‑764) trs56 ← / → b1995 IS5 transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,065,8660TC100.0% 938.1 / NA 293intergenic (‑523/‑764)trs56/b1995IS5 transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (158/135);  total (158/135)

Only 100 of 296 total aligned reads displayed.

CCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAG  >  NC_000913/2065776‑2065955
                                                                                          |                                                                                         
ccATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAacac                                                                                         >  1:1443822/1‑93 (MQ=255)
ccATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAacac                                                                                         >  2:3581645/1‑93 (MQ=255)
 cATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACg                                                                                        >  2:6140437/1‑93 (MQ=255)
  aTTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGa                                                                                       >  2:526205/1‑93 (MQ=255)
   ttttCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGaa                                                                                      <  1:2463375/93‑1 (MQ=255)
    tttCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAg                                                                                     <  2:6962979/93‑1 (MQ=255)
     ttCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGt                                                                                    <  1:6703879/93‑1 (MQ=255)
      tCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGtt                                                                                   >  1:4642246/1‑93 (MQ=255)
      tCAGAAGCTCTCCAAGAAAGCGAATTCTGTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGtt                                                                                   <  1:271307/93‑1 (MQ=255)
        aGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGc                                                                                 >  1:677290/1‑93 (MQ=255)
          aaGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTc                                                                               <  2:4870917/93‑1 (MQ=255)
           aGCTCTCCAAGAAAGCGAATTCATTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCa                                                                              <  2:4162620/93‑1 (MQ=255)
                 ccAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCa                                                                        >  2:5818760/1‑93 (MQ=255)
                  cAAGAAGACGAATTCTTATCTCAAATTCTGCATTATCAGACACAGCTTTCTACTCCATGGTAATGGCCAAAACACGAAGATGCTCAGGATCaa                                                                       <  2:7010480/93‑2 (MQ=255)
                  cAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCAt                                                                       <  1:1939047/93‑1 (MQ=255)
                   aaGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCAtt                                                                      >  2:5207890/1‑93 (MQ=255)
                    aGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTa                                                                     <  2:4494471/93‑1 (MQ=255)
                    aGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTa                                                                     <  2:5304598/93‑1 (MQ=255)
                    aGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTa                                                                     >  1:4253920/1‑93 (MQ=255)
                     gAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAg                                                                    >  1:1088007/1‑93 (MQ=255)
                      aaaGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgt                                                                   >  1:4363948/1‑93 (MQ=255)
                      aaaGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgt                                                                   <  1:930628/93‑1 (MQ=255)
                       aaGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgtg                                                                  >  1:2790540/1‑93 (MQ=255)
                       aaGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACCCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgtg                                                                  <  1:2262021/93‑1 (MQ=255)
                        aGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgtgg                                                                 <  2:3816264/93‑1 (MQ=255)
                        aGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgtgg                                                                 <  1:2414383/93‑1 (MQ=255)
                         gCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgtggt                                                                >  1:7582437/1‑93 (MQ=255)
                         gCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAgtggt                                                                <  2:6225788/93‑1 (MQ=255)
                           gAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGc                                                              <  2:3115482/93‑1 (MQ=255)
                            aaTTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCt                                                             >  2:3894177/1‑93 (MQ=255)
                            aaTTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCt                                                             >  2:6772904/1‑93 (MQ=255)
                            aaTTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCt                                                             >  2:1697719/1‑93 (MQ=255)
                            aaTTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCt                                                             <  2:2678209/93‑1 (MQ=255)
                            aaTTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCt                                                             >  2:3434890/1‑93 (MQ=255)
                            aaTTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCt                                                             >  1:5022096/1‑93 (MQ=255)
                              ttCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTga                                                           <  2:6502123/93‑1 (MQ=255)
                              ttCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGACCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTga                                                           >  1:3557083/1‑93 (MQ=255)
                               tCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTgag                                                          >  2:316734/1‑93 (MQ=255)
                               tCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTgag                                                          >  1:7335103/1‑93 (MQ=255)
                               tCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTgag                                                          <  1:4943384/93‑1 (MQ=255)
                                cTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTgaga                                                         >  1:4494584/1‑93 (MQ=255)
                                 ttttCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGaa                                                        >  2:6875647/1‑93 (MQ=255)
                                   ttCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGt                                                      <  1:6324842/93‑1 (MQ=255)
                                   ttCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGt                                                      >  1:2979128/1‑93 (MQ=255)
                                        aaaTTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAAt                                                 <  1:7191046/93‑1 (MQ=255)
                                        aaaTTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAAt                                                 >  2:7164690/1‑93 (MQ=255)
                                        aaaTTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAAt                                                 <  2:2891034/93‑1 (MQ=255)
                                          aTTCTGCATTCTCAGACACAGCTTTCTGCTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATaa                                               <  2:640966/93‑1 (MQ=255)
                                            tCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAAcc                                             >  2:2271255/1‑93 (MQ=255)
                                             cTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCa                                            <  1:7925112/93‑1 (MQ=255)
                                             cTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCa                                            >  1:563854/1‑93 (MQ=255)
                                             cTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCa                                            <  2:5559518/93‑1 (MQ=255)
                                             cTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCa                                            <  1:2507503/93‑1 (MQ=255)
                                              tGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAt                                           >  1:660613/1‑93 (MQ=255)
                                              tGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAt                                           <  2:1942774/93‑1 (MQ=255)
                                              tGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAt                                           <  2:5545845/93‑1 (MQ=255)
                                               gCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATg                                          <  1:1347783/93‑1 (MQ=255)
                                                cATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGc                                         >  2:2616770/1‑93 (MQ=255)
                                                 aTTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCg                                        >  1:5072459/1‑93 (MQ=255)
                                                   tctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGa                                      <  1:5035387/93‑1 (MQ=255)
                                                   tctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGa                                      <  2:4704409/93‑1 (MQ=255)
                                                   tctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGa                                      <  1:3471982/93‑1 (MQ=255)
                                                   tctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGa                                      <  2:4627796/93‑1 (MQ=255)
                                                   tctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGa                                      <  2:1297299/93‑1 (MQ=255)
                                                    ctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAg                                     <  2:5901347/93‑1 (MQ=255)
                                                    ctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAg                                     <  2:1527592/93‑1 (MQ=255)
                                                    ctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAg                                     <  1:3749518/93‑1 (MQ=255)
                                                    ctcAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAg                                     <  1:2784165/93‑1 (MQ=255)
                                                       aGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGCTCATTAGCGGTGCTGAGAAGTTAAATAACCATGCGGAGTAc                                  >  1:6856908/1‑93 (MQ=255)
                                                        gACACAGCTTTCTACTCCATGGTCATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAcc                                 <  2:5665140/93‑1 (MQ=255)
                                                        gACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAcc                                 >  2:5348685/1‑93 (MQ=255)
                                                        gACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAcc                                 >  2:7525131/1‑93 (MQ=255)
                                                        gACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGGTAAATAACCATGCGGAGTAcc                                 >  1:1706237/1‑93 (MQ=255)
                                                         acacAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCa                                <  1:5951366/93‑1 (MQ=255)
                                                          cacaGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCAc                               <  2:2772/93‑1 (MQ=255)
                                                          cacaGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCAc                               <  1:396025/93‑1 (MQ=255)
                                                           acaGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACt                              >  2:1820907/1‑93 (MQ=255)
                                                            caGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTc                             >  1:7270569/1‑93 (MQ=255)
                                                            caGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTc                             <  2:1054966/93‑1 (MQ=255)
                                                              gCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGt                           >  1:7413819/1‑93 (MQ=255)
                                                                tttCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGtca                         >  1:2151910/1‑93 (MQ=255)
                                                                  tCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGtcatc                       >  1:2012682/1‑93 (MQ=255)
                                                                    tACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCAc                     <  1:1240388/93‑1 (MQ=255)
                                                                     aCTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACg                    >  2:4918673/1‑93 (MQ=255)
                                                                       tCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTa                  <  2:3301623/93‑1 (MQ=255)
                                                                        ccATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTAt                 >  2:1391540/1‑93 (MQ=255)
                                                                           tGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTta              <  2:6329358/93‑1 (MQ=255)
                                                                           tGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTta              <  2:7388386/93‑1 (MQ=255)
                                                                              tAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTtatat           <  2:730882/93‑1 (MQ=255)
                                                                               aaTGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTtatata          <  2:6616956/93‑1 (MQ=255)
                                                                                  ggCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCa       >  2:216920/1‑93 (MQ=255)
                                                                                  ggCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCa       <  1:6940633/93‑1 (MQ=255)
                                                                                   gCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCAt      <  2:813968/93‑1 (MQ=255)
                                                                                    cccAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATg     <  2:2076243/93‑1 (MQ=255)
                                                                                     ccAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGa    <  1:7774656/93‑1 (MQ=255)
                                                                                      cAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGaa   >  2:6911271/1‑93 (MQ=255)
                                                                                      cAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGaa   <  1:987715/93‑1 (MQ=255)
                                                                                      cAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGaa   <  2:7795838/93‑1 (MQ=255)
                                                                                       aaaCACGAAGTTGCTCAGGGTCATTAGTGGTGCTGAGAAGATAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATAATGAAg  >  1:5566291/1‑93 (MQ=255)
                                                                                       aaaCACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAg  >  1:6033664/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAG  >  NC_000913/2065776‑2065955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGCCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  >  NC_000913/2065773‑2065959
                                                                                             |                                                                                             
GTGCCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAAC                                                                                               >  SRR4302543.2923608/1‑94 (MQ=60)
GTGCCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAAC                                                                                               <  SRR4302543.3241184/94‑1 (MQ=60)
 TGCCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACA                                                                                              >  SRR4302543.2337385/1‑94 (MQ=60)
 TGCCATTTTCAGAAGCTCACCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACA                                                                                              <  SRR4302543.944693/94‑1 (MQ=60)
  GCCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACAC                                                                                             >  SRR4302543.1456866/1‑94 (MQ=60)
   CCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACG                                                                                            <  SRR4302543.126702/94‑1 (MQ=60)
   CCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACG                                                                                            >  SRR4302543.6607203/1‑94 (MQ=60)
     ATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAA                                                                                          >  SRR4302543.2344901/1‑94 (MQ=60)
     ATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAA                                                                                          >  SRR4302543.7461335/1‑94 (MQ=60)
      TTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAG                                                                                         <  SRR4302543.2485591/94‑1 (MQ=60)
      TTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAG                                                                                         >  SRR4302543.218608/1‑94 (MQ=60)
        TTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTT                                                                                       <  SRR4302543.2268510/94‑1 (MQ=60)
        TTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTT                                                                                       <  SRR4302543.6767163/94‑1 (MQ=60)
        TTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTT                                                                                       >  SRR4302543.4684477/1‑94 (MQ=60)
         TCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTG                                                                                      <  SRR4302543.273693/94‑1 (MQ=60)
         TCAGAAGCTCTCCAAGAAAGCGAATTCTGTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTG                                                                                      <  SRR4302543.273692/94‑1 (MQ=60)
          CAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGC                                                                                     >  SRR4302543.354430/1‑94 (MQ=60)
          CAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGC                                                                                     >  SRR4302543.683525/1‑94 (MQ=60)
          CAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGC                                                                                     >  SRR4302543.695888/1‑94 (MQ=60)
           AGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCT                                                                                    >  SRR4302543.2025028/1‑94 (MQ=60)
            GAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTC                                                                                   >  SRR4302543.5655211/1‑94 (MQ=60)
            GAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTC                                                                                   <  SRR4302543.7417208/94‑1 (MQ=60)
             AAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCA                                                                                  >  SRR4302543.6486794/1‑94 (MQ=60)
              AGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAG                                                                                 >  SRR4302543.3560069/1‑94 (MQ=60)
              AGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAG                                                                                 <  SRR4302543.1702559/94‑1 (MQ=60)
              AGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAG                                                                                 >  SRR4302543.4477177/1‑94 (MQ=60)
              AGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAG                                                                                 <  SRR4302543.7767616/94‑1 (MQ=60)
                 TCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGAT                                                                              <  SRR4302543.1965475/94‑1 (MQ=60)
                  CTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATC                                                                             >  SRR4302543.6790927/1‑94 (MQ=60)
                  CTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATC                                                                             >  SRR4302543.5350495/1‑94 (MQ=60)
                    CCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCAT                                                                           <  SRR4302543.2402776/94‑1 (MQ=60)
                     CAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATT                                                                          >  SRR4302543.7850234/1‑94 (MQ=60)
                     CAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATT                                                                          <  SRR4302543.1956470/94‑1 (MQ=60)
                     CAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATA                                                                          >  SRR4302543.6841496/1‑94 (MQ=60)
                      AAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTA                                                                         >  SRR4302543.4292534/1‑94 (MQ=60)
                       AGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAG                                                                        <  SRR4302543.1905467/94‑1 (MQ=60)
                       AGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAG                                                                        >  SRR4302543.1097780/1‑94 (MQ=60)
                        GAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGT                                                                       >  SRR4302543.4403478/1‑94 (MQ=60)
                         AAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTG                                                                      <  SRR4302543.938764/94‑1 (MQ=60)
                         AAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTG                                                                      >  SRR4302543.2816021/1‑94 (MQ=60)
                          AAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACCCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGG                                                                     <  SRR4302543.2282652/94‑1 (MQ=60)
                           AGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGT                                                                    <  SRR4302543.2436197/94‑1 (MQ=60)
                           AGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGT                                                                    >  SRR4302543.7654515/1‑94 (MQ=60)
                            GCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTG                                                                   >  SRR4302543.4989388/1‑94 (MQ=60)
                             CGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGC                                                                  >  SRR4302543.6793170/1‑94 (MQ=60)
                              GAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCT                                                                 >  SRR4302543.5067800/1‑94 (MQ=60)
                                ATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGACCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGA                                                               >  SRR4302543.3588600/1‑94 (MQ=60)
                                 TTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAG                                                              >  SRR4302543.1200031/1‑94 (MQ=60)
                                 TTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAG                                                              >  SRR4302543.7405677/1‑94 (MQ=60)
                                  TCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGA                                                             <  SRR4302543.4988395/94‑1 (MQ=60)
                                  TCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGA                                                             >  SRR4302543.6747040/1‑94 (MQ=60)
                                  TCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGA                                                             >  SRR4302543.4535433/1‑94 (MQ=60)
                                    TTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAG                                                           <  SRR4302543.5085894/94‑1 (MQ=60)
                                     TTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGT                                                          >  SRR4302543.3006179/1‑94 (MQ=60)
                                     TTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGT                                                          >  SRR4302543.3676353/1‑94 (MQ=60)
                                      TTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTT                                                         <  SRR4302543.6384327/94‑1 (MQ=60)
                                        CTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCACACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAA                                                       >  SRR4302543.5101975/1‑94 (MQ=60)
                                        CTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAA                                                       >  SRR4302543.4618127/1‑94 (MQ=60)
                                        CTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAA                                                       >  SRR4302543.3631653/1‑94 (MQ=60)
                                         TCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAA                                                      <  SRR4302543.244667/94‑1 (MQ=60)
                                           AAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATA                                                    <  SRR4302543.2465948/94‑1 (MQ=60)
                                           AAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATA                                                    <  SRR4302543.7260789/94‑1 (MQ=60)
                                           AAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATA                                                    >  SRR4302543.7574876/1‑94 (MQ=60)
                                           AAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATA                                                    >  SRR4302543.7544303/1‑94 (MQ=60)
                                           AAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATA                                                    <  SRR4302543.249108/94‑1 (MQ=60)
                                             ATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAAC                                                  >  SRR4302543.7296808/1‑94 (MQ=60)
                                              TTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACC                                                 <  SRR4302543.4050517/94‑1 (MQ=60)
                                              TTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACC                                                 >  SRR4302543.6021392/1‑94 (MQ=60)
                                              TTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACA                                                 >  SRR4302543.2584517/1‑94 (MQ=60)
                                               TCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCA                                                >  SRR4302543.599165/1‑94 (MQ=60)
                                               TCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCA                                                >  SRR4302543.333623/1‑94 (MQ=60)
                                               TCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCA                                                >  SRR4302543.569022/1‑94 (MQ=60)
                                                CTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAT                                               >  SRR4302543.666683/1‑94 (MQ=60)
                                                CTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAT                                               <  SRR4302543.7999539/94‑1 (MQ=60)
                                                CTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAT                                               >  SRR4302543.938881/1‑94 (MQ=60)
                                                CTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCAT                                               <  SRR4302543.2530270/94‑1 (MQ=60)
                                                 TGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATG                                              <  SRR4302543.7848637/94‑1 (MQ=60)
                                                 TGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATG                                              <  SRR4302543.5645991/94‑1 (MQ=60)
                                                 TGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATG                                              <  SRR4302543.483882/94‑1 (MQ=60)
                                                  GCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGC                                             <  SRR4302543.1359832/94‑1 (MQ=60)
                                                   CATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCG                                            >  SRR4302543.1396141/1‑94 (MQ=60)
                                                   CATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCG                                            >  SRR4302543.5118495/1‑94 (MQ=60)
                                                    ATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGG                                           >  SRR4302543.7314461/1‑94 (MQ=60)
                                                    ATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGG                                           >  SRR4302543.4645840/1‑94 (MQ=60)
                                                    ATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGG                                           >  SRR4302543.1664439/1‑94 (MQ=60)
                                                      TCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAG                                         <  SRR4302543.5081190/94‑1 (MQ=60)
                                                      TCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAG                                         <  SRR4302543.3503081/94‑1 (MQ=60)
                                                       CTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGT                                        <  SRR4302543.2809559/94‑1 (MQ=60)
                                                       CTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGT                                        <  SRR4302543.2809560/94‑1 (MQ=60)
                                                       CTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGT                                        <  SRR4302543.3782508/94‑1 (MQ=60)
                                                        TCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAcccaggcgaagac                                       >  SRR4302543.5572325/1‑81 (MQ=60)
                                                         CAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGCTCATTAGCGGTGCTGAGAAGTTAAATAACCATGCGGAGTAC                                      >  SRR4302543.6921555/1‑94 (MQ=60)
                                                         CAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAC                                      <  SRR4302543.185663/94‑1 (MQ=60)
                                                         CAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAC                                      >  SRR4302543.4102958/1‑94 (MQ=60)
                                                         CAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAC                                      >  SRR4302543.5046515/1‑94 (MQ=60)
                                                         CAGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTAC                                      >  SRR4302543.7677788/1‑94 (MQ=60)
                                                          AGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGGTAAATAACCATGCGGAGTACC                                     >  SRR4302543.1721511/1‑94 (MQ=60)
                                                          AGACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCACGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACC                                     >  SRR4302543.4343429/1‑94 (MQ=60)
                                                           GACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCA                                    >  SRR4302543.442217/1‑94 (MQ=60)
                                                            ACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCAC                                   >  SRR4302543.6858897/1‑94 (MQ=60)
                                                            ACACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCAC                                   <  SRR4302543.6005829/94‑1 (MQ=60)
                                                             CACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACT                                  <  SRR4302543.399684/94‑1 (MQ=60)
                                                             CACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACT                                  <  SRR4302543.1365153/94‑1 (MQ=60)
                                                             CACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACT                                  >  SRR4302543.282774/1‑94 (MQ=60)
                                                              ACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTC                                 >  SRR4302543.7340806/1‑94 (MQ=60)
                                                              ACAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTC                                 >  SRR4302543.937319/1‑94 (MQ=60)
                                                               CAGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCG                                <  SRR4302543.6731499/94‑1 (MQ=60)
                                                                AGCTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGT                               >  SRR4302543.7484948/1‑94 (MQ=60)
                                                                  CTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCA                             >  SRR4302543.2171179/1‑94 (MQ=60)
                                                                  CTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCA                             >  SRR4302543.5847764/1‑94 (MQ=60)
                                                                  CTTTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACGCGTCA                             <  SRR4302543.5079392/94‑1 (MQ=60)
                                                                   TTTCTACTCCATGGTAATGACCCAAACACGAAGTTGCTCAGGATCGTTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTCCCACTCGTCGT                            <  SRR4302543.7109953/94‑1 (MQ=60)
                                                                    TTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATC                           >  SRR4302543.2030773/1‑94 (MQ=60)
                                                                    TTCTACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATC                           <  SRR4302543.6926287/94‑1 (MQ=60)
                                                                      CTACTCCATGGTAATGCCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCAC                         <  SRR4302543.2892948/94‑1 (MQ=60)
                                                                       TACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACG                        >  SRR4302543.869298/1‑94 (MQ=60)
                                                                       TACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACG                        <  SRR4302543.1251537/94‑1 (MQ=60)
                                                                        ACTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGT                       >  SRR4302543.2511236/1‑94 (MQ=60)
                                                                         CTCCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTA                      >  SRR4302543.7397649/1‑94 (MQ=60)
                                                                           CCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATT                    <  SRR4302543.4342617/94‑1 (MQ=60)
                                                                           CCATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTAATCACGTATT                    >  SRR4302543.2619441/1‑94 (MQ=60)
                                                                             ATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTA                  <  SRR4302543.1294479/94‑1 (MQ=60)
                                                                             ATGGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTA                  >  SRR4302543.4828996/1‑94 (MQ=60)
                                                                               GGTAATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTCTTTATA                >  SRR4302543.3860095/1‑94 (MQ=60)
                                                                                  AATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATAT             <  SRR4302543.970518/94‑1 (MQ=60)
                                                                                   ATGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATC            <  SRR4302543.317614/94‑1 (MQ=60)
                                                                                    TGGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCA           <  SRR4302543.3806989/94‑1 (MQ=60)
                                                                                     GGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTCTTTATATATCAT          >  SRR4302543.207363/1‑94 (MQ=60)
                                                                                     GGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCAT          >  SRR4302543.718950/1‑94 (MQ=60)
                                                                                     GGCCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCAT          <  SRR4302543.7005918/94‑1 (MQ=60)
                                                                                     GGCCCAAACACGAAGTTGCTCAGCATCATTAGTGGCGCTGAGCAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCAT          >  SRR4302543.207362/1‑94 (MQ=60)
                                                                                       CCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGA        >  SRR4302543.7346762/1‑94 (MQ=60)
                                                                                       CCCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGA        >  SRR4302543.2812609/1‑94 (MQ=60)
                                                                                        CCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAA       <  SRR4302543.6377429/94‑1 (MQ=60)
                                                                                        CCAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAA       <  SRR4302543.7847984/94‑1 (MQ=60)
                                                                                         CAAACACGAAGTTGCTCAGGGTCATTAGTGGTGCTGAGAAGATAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATAATGAAG      >  SRR4302543.5616926/1‑94 (MQ=60)
                                                                                         CAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAG      <  SRR4302543.996430/94‑1 (MQ=60)
                                                                                         CAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAG      >  SRR4302543.6090318/1‑94 (MQ=60)
                                                                                         CAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAG      >  SRR4302543.6090319/1‑94 (MQ=60)
                                                                                         CAAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAG      >  SRR4302543.2754689/1‑94 (MQ=60)
                                                                                          AAACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGC     <  SRR4302543.6297592/94‑1 (MQ=60)
                                                                                           AACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCT    >  SRR4302543.1912635/1‑94 (MQ=60)
                                                                                           AACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCT    <  SRR4302543.4799735/94‑1 (MQ=60)
                                                                                            ACACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTA   >  SRR4302543.6358407/1‑94 (MQ=60)
                                                                                             CACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  >  SRR4302543.5607348/1‑94 (MQ=60)
                                                                                             CACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  <  SRR4302543.2075025/94‑1 (MQ=60)
                                                                                             CACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  <  SRR4302543.3907363/94‑1 (MQ=60)
                                                                                             CACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCACGAAGCTAT  >  SRR4302543.3264119/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTGCCATTTTCAGAAGCTCTCCAAGAAAGCGAATTCTTTTCTCAAATTCTGCATTCTCAGACACAGCTTTCTACTCCATGGTAATGGCCCAAATACGAAGTTGCTCAGGATCATTAGTGGTGCTGAGAAGTTAAATAACCATGCGGAGTACCACTCGTCATCACGTATTTATATATCATGAAGCTAT  >  NC_000913/2065773‑2065959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑