Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,071,598 A→G R680G (AGG→GGG)  b2000 → outer membrane fluffing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,071,5980AG99.5% 678.6 / ‑5.1 218R680G (AGG→GGG) b2000outer membrane fluffing protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (106/111);  total (106/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 223 total aligned reads displayed.

TCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACAGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAA  >  NC_000913/2071508‑2071687
                                                                                          |                                                                                         
tCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACggg                                                                                         <  2:3327693/93‑1 (MQ=255)
 cGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGTGCCTTTGTCCAGGGGAACGGGc                                                                                        <  2:3315215/93‑1 (MQ=255)
 cGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGc                                                                                        <  2:4755251/93‑1 (MQ=255)
 cGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGc                                                                                        <  2:5111363/93‑1 (MQ=255)
 cGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGc                                                                                        <  2:5508877/93‑1 (MQ=255)
 cGGGGCGGGCGACCAGCGGTAGGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTGTGTCCAGGGGAACGGGc                                                                                        <  2:5111361/93‑1 (MQ=255)
   gggCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTg                                                                                      <  2:7165760/93‑1 (MQ=255)
    ggCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGc                                                                                     >  1:1307325/1‑93 (MQ=255)
    ggCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGc                                                                                     <  2:6235248/93‑1 (MQ=255)
     gCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCa                                                                                    <  1:909361/93‑1 (MQ=255)
     gCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCa                                                                                    <  1:949184/93‑1 (MQ=255)
        ggCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGTGAACGGGCTGCAGGc                                                                                 >  1:3686797/1‑93 (MQ=255)
        ggCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGc                                                                                 <  2:4221412/93‑1 (MQ=255)
        ggCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGc                                                                                 >  1:2271355/1‑93 (MQ=255)
        ggCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGc                                                                                 >  1:4724263/1‑93 (MQ=255)
            aCCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCggg                                                                             >  1:3622414/1‑92 (MQ=255)
             ccAGCGGTAAGGGTATTCAGGTGGTGGAAGCGATTAACGGTGACACCACTGAGGAAGTGGCCTCTGTCCAGGGGTACGGGGTGCACGCCGATg                                                                            >  1:5383572/1‑93 (MQ=255)
              cAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCATGGGAACGGGCTGCAGGCCGGTGc                                                                           >  2:4308525/1‑93 (MQ=255)
              cAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGc                                                                           >  1:7192097/1‑93 (MQ=255)
               aGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGcc                                                                          <  1:7068526/93‑1 (MQ=255)
               aGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGcc                                                                          >  1:482012/1‑93 (MQ=255)
                gCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCt                                                                         <  2:1928615/93‑1 (MQ=255)
                gCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCt                                                                         <  1:6303183/93‑1 (MQ=255)
                 cGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCtt                                                                        >  1:3130794/1‑93 (MQ=255)
                 cGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCtt                                                                        <  1:393087/93‑1 (MQ=255)
                 cGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCtt                                                                        <  2:5438917/93‑1 (MQ=255)
                  ggTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCttt                                                                       >  2:3915575/1‑93 (MQ=255)
                  ggTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCttt                                                                       >  1:543107/1‑93 (MQ=255)
                   gTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTa                                                                      >  2:7697223/1‑93 (MQ=255)
                   gTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTa                                                                      >  1:556179/1‑93 (MQ=255)
                   gTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTa                                                                      >  1:5773521/1‑93 (MQ=255)
                     aaGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAac                                                                    <  1:6702207/93‑1 (MQ=255)
                        ggTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAactac                                                                 <  2:671843/93‑1 (MQ=255)
                          tATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTc                                                               >  1:6208358/1‑93 (MQ=255)
                           aTTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTcc                                                              >  1:3672097/1‑93 (MQ=255)
                           aTTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTcc                                                              <  2:1617584/93‑1 (MQ=255)
                             tCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCt                                                            >  2:7373879/1‑93 (MQ=255)
                             tCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCt                                                            >  2:3036251/1‑93 (MQ=255)
                              cAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTc                                                           >  1:1606558/1‑93 (MQ=255)
                               aGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCa                                                          <  1:3276563/93‑1 (MQ=255)
                               aGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCa                                                          >  2:6420233/1‑93 (MQ=255)
                               aGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCa                                                          >  2:5328665/1‑93 (MQ=255)
                                 gtggtgGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAAc                                                        >  1:649845/1‑93 (MQ=255)
                                   ggtggAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCg                                                      >  1:6950269/1‑93 (MQ=255)
                                    gtggAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCgg                                                     <  2:3800581/93‑1 (MQ=255)
                                    gtggAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCgg                                                     >  2:7890102/1‑93 (MQ=255)
                                      ggAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGa                                                   <  2:2767533/93‑1 (MQ=255)
                                       gAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGAc                                                  <  1:4761752/93‑1 (MQ=255)
                                          gCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGt                                               <  1:4284115/93‑1 (MQ=255)
                                            cATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGGACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtga                                             >  2:338573/1‑93 (MQ=255)
                                             aTTAACGGTGCCACCACGGAGTAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtgat                                            >  1:1410766/1‑93 (MQ=255)
                                             aTTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTTCTCCCTCAACCGGGACAGtgat                                            <  2:4806968/93‑1 (MQ=255)
                                             aTTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtgat                                            >  2:7542648/1‑93 (MQ=255)
                                              ttAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtgatg                                           >  2:2330710/1‑93 (MQ=255)
                                              ttAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtgatg                                           >  2:3845767/1‑93 (MQ=255)
                                              ttAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtgatg                                           >  1:4048588/1‑93 (MQ=255)
                                              ttAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGtgatg                                           >  1:7362382/1‑93 (MQ=255)
                                                    gTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCt                                     <  2:2303805/93‑1 (MQ=255)
                                                    gTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCt                                     <  1:6336237/93‑1 (MQ=255)
                                                    gTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCt                                     <  2:4674797/93‑1 (MQ=255)
                                                      gCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGTCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTgg                                   <  1:4038864/93‑1 (MQ=255)
                                                      gCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTgg                                   >  2:5730639/1‑93 (MQ=255)
                                                      gCCACCACGGAGGAAGGGGCCTTGGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTgg                                   <  1:5901393/93‑1 (MQ=255)
                                                        caccacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTa                                 >  1:6826425/1‑93 (MQ=255)
                                                         cccacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTAt                                <  2:1768075/92‑1 (MQ=255)
                                                         accacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTAt                                <  1:4897114/93‑1 (MQ=255)
                                                          ccacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATc                               <  1:7439424/93‑1 (MQ=255)
                                                          ccacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATc                               <  1:5116996/93‑1 (MQ=255)
                                                           cacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCt                              >  1:5685111/1‑93 (MQ=255)
                                                           cacGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCt                              <  2:2853084/93‑1 (MQ=255)
                                                            acGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTg                             >  2:4479510/1‑93 (MQ=255)
                                                            acGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTg                             >  2:1244392/1‑93 (MQ=255)
                                                             cGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTgc                            <  1:1932005/93‑1 (MQ=255)
                                                             cGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTgc                            >  2:7490726/1‑93 (MQ=255)
                                                               gaggaAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTgcgc                          <  2:2798469/93‑1 (MQ=255)
                                                               gagcaAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTgcgc                          <  1:5975560/93‑1 (MQ=255)
                                                                aggaAGGTGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCa                         <  1:3369026/93‑1 (MQ=255)
                                                                 ggaAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAg                        >  1:1797796/1‑93 (MQ=255)
                                                                     ggggCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGaa                    >  1:4872977/1‑93 (MQ=255)
                                                                      gggCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGaaa                   >  1:1242614/1‑93 (MQ=255)
                                                                       ggacTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGaaaa                  <  1:2736469/90‑1 (MQ=255)
                                                                       ggCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGaaaa                  <  2:6108385/93‑1 (MQ=255)
                                                                        gCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAAt                 >  2:7079349/1‑93 (MQ=255)
                                                                        gCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAAt                 >  1:7613262/1‑93 (MQ=255)
                                                                         ccTTTGTCCAGGGGAACGGGCTGCAGGCCGGGGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATg                >  2:5611962/1‑93 (MQ=255)
                                                                          cTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGc               <  2:5853373/93‑1 (MQ=255)
                                                                           tttGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCt              >  2:6913344/1‑93 (MQ=255)
                                                                             tGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTa            >  2:4556266/1‑93 (MQ=255)
                                                                              gTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTAt           <  1:1354512/93‑1 (MQ=255)
                                                                               tCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGCGAAAATGCTTATc          <  2:3723577/93‑1 (MQ=255)
                                                                                 cAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGt        >  1:7001729/1‑93 (MQ=255)
                                                                                  aGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTg       >  2:2736717/1‑93 (MQ=255)
                                                                                  aGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTg       <  2:1002759/93‑1 (MQ=255)
                                                                                   ggggAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGc      >  1:3608915/1‑93 (MQ=255)
                                                                                    gggAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCa     <  2:6533627/93‑1 (MQ=255)
                                                                                    gggAACGGGCTGCAGGCCGGTGCCTTAAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCa     <  2:3894677/93‑1 (MQ=255)
                                                                                     ggAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAg    >  2:4779681/1‑93 (MQ=255)
                                                                                       aaCGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGaa  <  2:4872920/93‑1 (MQ=255)
                                                                                       aaCGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGaa  <  1:2488882/93‑1 (MQ=255)
                                                                                       aaCGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGaa  <  1:235050/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACAGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAA  >  NC_000913/2071508‑2071687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACAGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTCC  >  NC_000913/2071506‑2071691
                                                                                            |                                                                                             
CGTCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGG                                                                                              >  SRR4302543.4623810/1‑94 (MQ=60)
     GGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGC                                                                                         >  SRR4302543.1318936/1‑94 (MQ=60)
     GGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGC                                                                                         >  SRR4302543.3688095/1‑94 (MQ=60)
       GCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAG                                                                                       <  SRR4302543.957473/94‑1 (MQ=60)
       GCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAG                                                                                       <  SRR4302543.917357/94‑1 (MQ=60)
        CTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGC                                                                                      >  SRR4302543.4948748/1‑94 (MQ=60)
         TGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGTGAACGGGCTGCAGGC                                                                                     >  SRR4302543.3719191/1‑94 (MQ=60)
         TGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGC                                                                                     >  SRR4302543.2292112/1‑94 (MQ=60)
         TGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGC                                                                                     >  SRR4302543.4767424/1‑94 (MQ=60)
          GGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCC                                                                                    <  SRR4302543.926358/94‑1 (MQ=60)
          GGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCC                                                                                    <  SRR4302543.5724459/94‑1 (MQ=60)
            GGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGG                                                                                  <  SRR4302543.4849551/94‑1 (MQ=60)
            CGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGG                                                                                  >  SRR4302543.6376759/1‑94 (MQ=60)
            CGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGG                                                                                  >  SRR4302543.7509806/1‑94 (MQ=60)
             GACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGG                                                                                 >  SRR4302543.3654332/1‑94 (MQ=60)
               CCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGC                                                                               >  SRR4302543.7261848/1‑94 (MQ=60)
               CCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGC                                                                               <  SRR4302543.1257014/94‑1 (MQ=60)
               CCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGC                                                                               >  SRR4302543.1516426/1‑94 (MQ=60)
                CAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCC                                                                              >  SRR4302543.486467/1‑94 (MQ=60)
                 AGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCT                                                                             <  SRR4302543.7137158/94‑1 (MQ=60)
                  GCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTT                                                                            <  SRR4302543.6362485/94‑1 (MQ=60)
                  GCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTT                                                                            >  SRR4302543.3158952/1‑94 (MQ=60)
                   GGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTT                                                                           <  SRR4302543.7251623/94‑1 (MQ=60)
                   CGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTT                                                                           <  SRR4302543.396716/94‑1 (MQ=60)
                   CGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTT                                                                           >  SRR4302543.548125/1‑94 (MQ=60)
                   CGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTT                                                                           >  SRR4302543.3731333/1‑94 (MQ=60)
                    GGTAAGGGTATTCAGGTGGTGGAAGGCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTA                                                                          >  SRR4302543.5633228/1‑94 (MQ=60)
                    GGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTA                                                                          >  SRR4302543.561284/1‑94 (MQ=60)
                    GGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTA                                                                          >  SRR4302543.5826075/1‑94 (MQ=60)
                      TAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAAC                                                                        <  SRR4302543.4387736/94‑1 (MQ=60)
                      TAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAAC                                                                        <  SRR4302543.7079318/94‑1 (MQ=60)
                       AAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACT                                                                       <  SRR4302543.6765461/94‑1 (MQ=60)
                       AAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACT                                                                       <  SRR4302543.7701900/94‑1 (MQ=60)
                        AGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTA                                                                      >  SRR4302543.364893/1‑94 (MQ=60)
                        AGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTA                                                                      <  SRR4302543.5116309/94‑1 (MQ=60)
                        AGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTA                                                                      >  SRR4302543.4583005/1‑94 (MQ=60)
                         GGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTAC                                                                     <  SRR4302543.7053336/94‑1 (MQ=60)
                           GTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTC                                                                   >  SRR4302543.3596398/1‑94 (MQ=60)
                           GTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTC                                                                   >  SRR4302543.6266976/1‑94 (MQ=60)
                            TATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCC                                                                  >  SRR4302543.3704336/1‑94 (MQ=60)
                              TTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCT                                                                >  SRR4302543.3368694/1‑94 (MQ=60)
                               TCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTC                                                               >  SRR4302543.1620888/1‑94 (MQ=60)
                                CAGGGGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCA                                                              >  SRR4302543.6841941/1‑94 (MQ=60)
                                 AGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAA                                                             <  SRR4302543.3306153/94‑1 (MQ=60)
                                 AGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAA                                                             >  SRR4302543.2294377/1‑94 (MQ=60)
                                  GGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAAC                                                            >  SRR4302543.5573402/1‑94 (MQ=60)
                                  GGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAAC                                                            >  SRR4302543.2760016/1‑94 (MQ=60)
                                  GGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAAC                                                            >  SRR4302543.655792/1‑94 (MQ=60)
                                    TGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCG                                                          >  SRR4302543.7015654/1‑94 (MQ=60)
                                     GGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGG                                                         <  SRR4302543.7620699/94‑1 (MQ=60)
                                      GTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGACTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGG                                                        >  SRR4302543.6077003/1‑94 (MQ=60)
                                        GGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGAC                                                      <  SRR4302543.3913890/94‑1 (MQ=60)
                                         GAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACA                                                     <  SRR4302543.4805263/94‑1 (MQ=60)
                                            GCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTG                                                  <  SRR4302543.6853816/94‑1 (MQ=60)
                                            GCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTG                                                  <  SRR4302543.4323050/94‑1 (MQ=60)
                                              CATTAACGGTGCCACCACGGAGTAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGAT                                                >  SRR4302543.1423516/1‑94 (MQ=60)
                                              CATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGAT                                                <  SRR4302543.7205139/94‑1 (MQ=60)
                                              CATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGAT                                                >  SRR4302543.1696074/1‑94 (MQ=60)
                                              CATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGAT                                                <  SRR4302543.3705060/94‑1 (MQ=60)
                                               ATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATG                                               >  SRR4302543.7433177/1‑94 (MQ=60)
                                               ATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATG                                               >  SRR4302543.4084816/1‑94 (MQ=60)
                                                  AACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACAGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGA                                            <  SRR4302543.5267625/94‑1 (MQ=60)
                                                   ACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAG                                           >  SRR4302543.5791998/1‑94 (MQ=60)
                                                    CGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGC                                          >  SRR4302543.1659723/1‑94 (MQ=60)
                                                    CGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGC                                          >  SRR4302543.5469033/1‑94 (MQ=60)
                                                      GTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTG                                        <  SRR4302543.6395826/94‑1 (MQ=60)
                                                       TGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGG                                       <  SRR4302543.5989565/94‑1 (MQ=60)
                                                        GCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGTCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGT                                      <  SRR4302543.4075009/94‑1 (MQ=60)
                                                        GCCACCACGGAGGAAGGGGCCTTGGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGT                                      <  SRR4302543.5955230/94‑1 (MQ=60)
                                                         CCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTA                                     >  SRR4302543.6890871/1‑94 (MQ=60)
                                                          CACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTAT                                    >  SRR4302543.4154024/1‑94 (MQ=60)
                                                          CACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTAT                                    >  SRR4302543.3431586/1‑94 (MQ=60)
                                                           ACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATC                                   <  SRR4302543.4941751/94‑1 (MQ=60)
                                                           ACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATC                                   <  SRR4302543.3259429/94‑1 (MQ=60)
                                                           ACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACACCCTCAACCGGGACAGTGATGAGAGCTGGTATC                                   >  SRR4302543.7701442/1‑94 (MQ=60)
                                                            CCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCT                                  <  SRR4302543.5163282/94‑1 (MQ=60)
                                                            CCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCT                                  <  SRR4302543.6485686/94‑1 (MQ=60)
                                                            CCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCT                                  >  SRR4302543.5736999/1‑94 (MQ=60)
                                                            CCACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCT                                  <  SRR4302543.7510729/94‑1 (MQ=60)
                                                             CACGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTG                                 >  SRR4302543.1347467/1‑94 (MQ=60)
                                                              ACGGAGGAAGGGGCCGTGGGCCAGGTGAACGGGCTGCAGGCCGGTGCCTTTAACTACCCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGC                                <  SRR4302543.982282/94‑1 (MQ=60)
                                                               CGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCG                               <  SRR4302543.1949385/94‑1 (MQ=60)
                                                               CGGAGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCG                               >  SRR4302543.4769624/1‑94 (MQ=60)
                                                                 GAGCAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCA                             <  SRR4302543.6030290/94‑1 (MQ=60)
                                                                  AGGAAGGTGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAG                            <  SRR4302543.3399291/94‑1 (MQ=60)
                                                                  AGGAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAG                            >  SRR4302543.1813896/1‑94 (MQ=60)
                                                                    GAAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTG                          <  SRR4302543.1851296/94‑1 (MQ=60)
                                                                     AAGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGA                         <  SRR4302543.6925300/94‑1 (MQ=60)
                                                                      AGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAA                        >  SRR4302543.6317675/1‑94 (MQ=60)
                                                                      AGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAA                        >  SRR4302543.4917398/1‑94 (MQ=60)
                                                                      AGGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAA                        >  SRR4302543.4917397/1‑94 (MQ=60)
                                                                       GGGGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAA                       >  SRR4302543.1253777/1‑94 (MQ=60)
                                                                         GGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAAT                     <  SRR4302543.5951519/94‑1 (MQ=60)
                                                                         GGCCTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAAT                     >  SRR4302543.7685549/1‑94 (MQ=60)
                                                                         GGACTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAAT                     <  SRR4302543.2761367/94‑1 (MQ=60)
                                                                            CTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCT                  >  SRR4302543.3980758/1‑94 (MQ=60)
                                                                            CTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCT                  >  SRR4302543.4079780/1‑94 (MQ=60)
                                                                            CTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCT                  <  SRR4302543.657687/94‑1 (MQ=60)
                                                                            CTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCT                  >  SRR4302543.2508787/1‑94 (MQ=60)
                                                                            CTTTGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACCCCCTCAACCGGtccaggggtgcgagcgggactctgcgcagtgaaattggt                  >  SRR4302543.2508786/1‑55 (MQ=60)
                                                                               TGTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTAT               <  SRR4302543.6097533/94‑1 (MQ=60)
                                                                                GTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATC              >  SRR4302543.1953195/1‑94 (MQ=60)
                                                                                GTCCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATC              <  SRR4302543.1366628/94‑1 (MQ=60)
                                                                                  CCAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGT            >  SRR4302543.7068132/1‑94 (MQ=60)
                                                                                   CAGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTG           <  SRR4302543.1417962/94‑1 (MQ=60)
                                                                                    AGGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGC          >  SRR4302543.3640753/1‑94 (MQ=60)
                                                                                     GGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCA         <  SRR4302543.2765564/94‑1 (MQ=60)
                                                                                     GGGGAACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCA         <  SRR4302543.7291428/94‑1 (MQ=60)
                                                                                         AACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAG     <  SRR4302543.2511432/94‑1 (MQ=60)
                                                                                         AACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAG     <  SRR4302543.237081/94‑1 (MQ=60)
                                                                                         AACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAG     <  SRR4302543.4916230/94‑1 (MQ=60)
                                                                                          ACGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGT    <  SRR4302543.3310617/94‑1 (MQ=60)
                                                                                           CGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTC   >  SRR4302543.5334846/1‑94 (MQ=60)
                                                                                           CGGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTC   >  SRR4302543.5334847/1‑94 (MQ=60)
                                                                                            GGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTCC  <  SRR4302543.1271781/94‑1 (MQ=60)
                                                                                            |                                                                                             
CGTCGGGGCTGGCGACCAGCGGTAAGGGTATTCAGGTGGTGGAAGCCATTAACGGTGCCACCACGGAGGAAGGGGCCTTTGTCCAGGGGAACAGGCTGCAGGCCGGTGCCTTTAACTACTCCCTCAACCGGGACAGTGATGAGAGCTGGTATCTGCGCAGTGAAAATGCTTATCGTGCAGAAGTCC  >  NC_000913/2071506‑2071691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑