Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,088,609	T→C	P132P (CCT→CCC)	hisG →	ATP phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,088,609	0	T	C	99.6%	787.8 / ‑4.9	249	P132P (CCT→CCC)	hisG	ATP phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (123/125); total (124/125)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

Only 100 of 253 total aligned reads displayed.

CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG  >  NC_000913/2088519‑2088698

 ggcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAc                                                                                        <  1:6566875/93‑1 (MQ=255)

 ggcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAc                                                                                        >  1:6212445/1‑93 (MQ=255)

  gcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAcc                                                                                       <  2:7719087/93‑1 (MQ=255)

  gcTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAcc                                                                                       >  2:5214478/1‑93 (MQ=255)

  gcTGTCGGCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAcc                                                                                       <  2:5715543/93‑1 (MQ=255)

     gtcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGc                                                                                    >  1:5431716/1‑93 (MQ=255)

     gtcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGc                                                                                    <  1:6079367/93‑1 (MQ=255)

     gtcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGc                                                                                    >  1:90554/1‑93 (MQ=255)

      tcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCt                                                                                   >  2:7361880/1‑93 (MQ=255)

      tcgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCt                                                                                   >  1:7662642/1‑93 (MQ=255)

       cgtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGCCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTc                                                                                  <  2:4982323/93‑1 (MQ=255)

        gtcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCa                                                                                 <  2:6303521/93‑1 (MQ=255)

         tcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCaa                                                                                <  1:1081227/93‑1 (MQ=255)

         tcTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCaa                                                                                <  1:1170477/93‑1 (MQ=255)

           tttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGc                                                                              <  1:3958164/93‑1 (MQ=255)

           tttCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGc                                                                              <  1:3937325/93‑1 (MQ=255)

              cGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGtt                                                                           <  1:6343655/93‑1 (MQ=255)

              cGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGtt                                                                           <  2:1065988/93‑1 (MQ=255)

                cTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAt                                                                         >  2:555818/1‑93 (MQ=255)

                cTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAt                                                                         <  2:6441841/93‑1 (MQ=255)

                 tGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtc                                                                        >  1:371376/1‑93 (MQ=255)

                  ggCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtct                                                                       >  2:2517094/1‑93 (MQ=255)

                   gCACCGCCGGTTGATGAAGCCTGGGACGGTCCTCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtctc                                                                      <  1:4397909/93‑1 (MQ=255)

                   gCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTAtctc                                                                      <  2:1196679/93‑1 (MQ=255)

                    cAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCg                                                                     >  1:178297/1‑93 (MQ=255)

                      aCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   <  1:2391140/93‑1 (MQ=255)

                      aCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   >  2:6651586/1‑93 (MQ=255)

                      aCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   <  1:4527035/93‑1 (MQ=255)

                      aCGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAc                                                                   >  1:277535/1‑93 (MQ=255)

                       agCCGGTTGATGAAGCCTAGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCCCCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAcc                                                                  <  2:7732346/92‑1 (MQ=255)

                        gCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCa                                                                 >  2:5907706/1‑93 (MQ=255)

                          cGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGa                                                               <  2:2381138/93‑1 (MQ=255)

                           ggTTGATGAAGCGTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaa                                                              <  2:4864550/93‑1 (MQ=255)

                           ggTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaa                                                              >  1:3399912/1‑93 (MQ=255)

                            gTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaaa                                                             >  1:1282508/1‑93 (MQ=255)

                            gTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGaaa                                                             <  2:3487094/93‑1 (MQ=255)

                            gTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATATCGACCAGaaa                                                             <  1:5697591/93‑1 (MQ=255)

                             ttGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAg                                                            <  2:6956136/93‑1 (MQ=255)

                              tgatgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAgg                                                           <  1:4911265/93‑1 (MQ=255)

                                atgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCa                                                         >  1:2652723/1‑93 (MQ=255)

                                atgaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCa                                                         >  1:4931648/1‑93 (MQ=255)

                                  gaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtc                                                       <  2:7280068/93‑1 (MQ=255)

                                  gaAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtc                                                       <  2:7923136/93‑1 (MQ=255)

                                    aGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtctc                                                     <  2:476794/93‑1 (MQ=255)

                                    aGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAtctc                                                     <  1:590550/93‑1 (MQ=255)

                                      ccTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtt                                                   <  1:805661/93‑1 (MQ=255)

                                      ccTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtt                                                   <  1:1075085/93‑1 (MQ=255)

                                        tGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtttt                                                 >  1:4673327/1‑93 (MQ=255)

                                        tGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTAGCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCtttt                                                 <  2:3557086/93‑1 (MQ=255)

                                         gggACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTa                                                <  2:5458161/93‑1 (MQ=255)

                                           gACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTaaa                                              <  1:6783294/93‑1 (MQ=255)

                                             cGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATc                                            <  2:334934/93‑1 (MQ=255)

                                             cGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATc                                            <  2:3000535/93‑1 (MQ=255)

                                             cGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATc                                            <  2:4173052/93‑1 (MQ=255)

                                              ggTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATcc                                           >  1:7133662/1‑93 (MQ=255)

                                               gTCTGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACGTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          >  2:2733826/1‑93 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  2:2167763/93‑1 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          <  2:2473063/93‑1 (MQ=255)

                                               gTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCt                                          >  2:5846056/1‑93 (MQ=255)

                                                tCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTg                                         <  2:5696580/93‑1 (MQ=255)

                                                tCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTg                                         <  1:229007/93‑1 (MQ=255)

                                                tCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTg                                         >  1:5178740/1‑93 (MQ=255)

                                                 ccGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGc                                        <  2:726303/93‑1 (MQ=255)

                                                  cGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTTAATCCTGCt                                       >  2:5379007/1‑93 (MQ=255)

                                                    ctctCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     >  1:1901913/1‑93 (MQ=255)

                                                    ctctCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTa                                     >  1:2100424/1‑93 (MQ=255)

                                                     tctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAc                                    >  1:2447235/1‑93 (MQ=255)

                                                     tctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAc                                    <  2:1900313/93‑1 (MQ=255)

                                                      ctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACt                                   >  1:6955461/1‑93 (MQ=255)

                                                      ctcCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACt                                   <  1:4249180/93‑1 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                >  1:6489229/1‑93 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                >  1:5793185/1‑93 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                >  1:7219431/1‑93 (MQ=255)

                                                         cTTAAACGGTAAACGTATCGCCACCTCTTATCACCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGaa                                >  1:6126204/1‑93 (MQ=255)

                                                           tAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                              >  1:5556303/1‑93 (MQ=255)

                                                           tAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                              >  1:1253253/1‑93 (MQ=255)

                                                           tAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACg                              >  2:1596303/1‑93 (MQ=255)

                                                            aaaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACgg                             <  2:1722431/93‑1 (MQ=255)

                                                             aaCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGt                            >  2:6505158/1‑93 (MQ=255)

                                                               cGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTc                          <  2:1350519/93‑1 (MQ=255)

                                                                ggTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCt                         <  1:7612194/93‑1 (MQ=255)

                                                                  tAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGt                       <  1:6846357/93‑1 (MQ=255)

                                                                  tAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGt                       >  1:3088185/1‑93 (MQ=255)

                                                                   aaaCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATATCTTTTAAATCCTGCTTACTGAACGGTTCTGtt                      >  2:6897237/1‑93 (MQ=255)

                                                                    aaCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTg                     >  1:6303687/1‑93 (MQ=255)

                                                                    aaCGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTg                     >  1:1208738/1‑93 (MQ=255)

                                                                      cGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGaa                   <  2:5761833/93‑1 (MQ=255)

                                                                      cGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGaa                   <  2:314209/93‑1 (MQ=255)

                                                                       gTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAg                  >  1:5864741/1‑93 (MQ=255)

                                                                       gTATCGCCACCTCTTATACCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAg                  >  2:4310196/1‑93 (MQ=255)

                                                                         aTCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTc                <  2:621705/93‑1 (MQ=255)

                                                                            gCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcc             >  2:6710332/1‑93 (MQ=255)

                                                                            gCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcc             <  2:5093371/93‑1 (MQ=255)

                                                                              cACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGctcc           >  2:2789857/1‑93 (MQ=255)

                                                                              cACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGcccc           <  1:1678802/93‑1 (MQ=255)

                                                                               aCCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcg          >  2:3954474/1‑93 (MQ=255)

                                                                                 ctctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCcgcg        <  2:7524337/93‑1 (MQ=255)

                                                                                  tctTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGt       >  2:1262417/1‑93 (MQ=255)

                                                                                    ttATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCTCCGCGTGc     >  2:397699/1‑93 (MQ=255)

                                                                                       tCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCgg  >  1:3906463/1‑93 (MQ=255)

CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG  >  NC_000913/2088519‑2088698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  NC_000913/2088516‑2088702

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCC                                                                                               >  SRR4302543.754320/1‑94 (MQ=60)

 GGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCC                                                                                              >  SRR4302543.3632560/1‑94 (MQ=60)

  GCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCA                                                                                             <  SRR4302543.2272995/94‑1 (MQ=60)

   CGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCAC                                                                                            >  SRR4302543.6271104/1‑94 (MQ=60)

    GGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACC                                                                                           <  SRR4302543.6628674/94‑1 (MQ=60)

      CTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTG                                                                                         <  SRR4302543.454032/94‑1 (MQ=60)

      CTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTG                                                                                         >  SRR4302543.4758327/1‑94 (MQ=60)

       TGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGC                                                                                        >  SRR4302543.91403/1‑94 (MQ=60)

       TGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGC                                                                                        >  SRR4302543.903799/1‑94 (MQ=60)

       TGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGC                                                                                        >  SRR4302543.5481067/1‑94 (MQ=60)

        GTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCT                                                                                       <  SRR4302543.6136982/94‑1 (MQ=60)

        GTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCT                                                                                       <  SRR4302543.6136983/94‑1 (MQ=60)

        GTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCT                                                                                       >  SRR4302543.7735268/1‑94 (MQ=60)

         TCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTC                                                                                      >  SRR4302543.4510359/1‑94 (MQ=60)

         TCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTC                                                                                      >  SRR4302543.4152228/1‑94 (MQ=60)

           GTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAA                                                                                    >  SRR4302543.6947298/1‑94 (MQ=60)

            TCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAG                                                                                   <  SRR4302543.1181105/94‑1 (MQ=60)

            TCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAG                                                                                   <  SRR4302543.1090947/94‑1 (MQ=60)

            TCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAG                                                                                   >  SRR4302543.6390702/1‑94 (MQ=60)

             CTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGC                                                                                  <  SRR4302543.1264304/94‑1 (MQ=60)

              TTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCG                                                                                 <  SRR4302543.3972118/94‑1 (MQ=60)

              TTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCG                                                                                 <  SRR4302543.5831240/94‑1 (MQ=60)

              TTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCG                                                                                 <  SRR4302543.3993200/94‑1 (MQ=60)

                TCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTT                                                                               <  SRR4302543.3181467/94‑1 (MQ=60)

                 CGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTA                                                                              <  SRR4302543.6403309/94‑1 (MQ=60)

                 CGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTA                                                                              <  SRR4302543.3150004/94‑1 (MQ=60)

                   CTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATC                                                                            >  SRR4302543.374797/1‑94 (MQ=60)

                   CTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATC                                                                            >  SRR4302543.7292876/1‑94 (MQ=60)

                   CTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATC                                                                            >  SRR4302543.5834300/1‑94 (MQ=60)

                    TGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCT                                                                           <  SRR4302543.3577752/94‑1 (MQ=60)

                    TGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCT                                                                           <  SRR4302543.6325522/94‑1 (MQ=60)

                      GCACCGCCGGTTGATGAAGCCTGGGACGGTCCTCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCG                                                                         <  SRR4302543.4437778/94‑1 (MQ=60)

                      GCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCG                                                                         >  SRR4302543.179818/1‑94 (MQ=60)

                      GCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCG                                                                         <  SRR4302543.2801961/94‑1 (MQ=60)

                        AACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAC                                                                       <  SRR4302543.6262998/94‑1 (MQ=60)

                        AACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAC                                                                       <  SRR4302543.6262997/94‑1 (MQ=60)

                        AACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGAC                                                                       >  SRR4302543.279990/1‑94 (MQ=60)

                         ACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACC                                                                      <  SRR4302543.4568231/94‑1 (MQ=60)

                         ACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACC                                                                      <  SRR4302543.2412777/94‑1 (MQ=60)

                           GCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAG                                                                    <  SRR4302543.5567302/94‑1 (MQ=60)

                            CCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGA                                                                   >  SRR4302543.4956706/1‑94 (MQ=60)

                             CGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAA                                                                  >  SRR4302543.3430371/1‑94 (MQ=60)

                             CGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAA                                                                  >  SRR4302543.5104033/1‑94 (MQ=60)

                              GGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAA                                                                 >  SRR4302543.1293945/1‑94 (MQ=60)

                               GTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAG                                                                <  SRR4302543.7822852/94‑1 (MQ=60)

                               GTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAG                                                                >  SRR4302543.7156627/1‑94 (MQ=60)

                               GTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATATCGACCAGAAAG                                                                <  SRR4302543.5749566/94‑1 (MQ=60)

                                TTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGG                                                               >  SRR4302543.1186049/1‑94 (MQ=60)

                                 TGATGAAGCCTGGGACGGTCCGCTCTCCTTAACCGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGC                                                              <  SRR4302543.2413513/94‑1 (MQ=60)

                                 TGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGC                                                              >  SRR4302543.1278558/1‑94 (MQ=60)

                                 TGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGC                                                              <  SRR4302543.4956028/94‑1 (MQ=60)

                                  GATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCGCCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCA                                                             <  SRR4302543.6381904/94‑1 (MQ=60)

                                  GATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCA                                                             >  SRR4302543.2676775/1‑94 (MQ=60)

                                  GATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCA                                                             >  SRR4302543.4976567/1‑94 (MQ=60)

                                  GATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCA                                                             <  SRR4302543.1912061/94‑1 (MQ=60)

                                   ATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCAT                                                            >  SRR4302543.3951723/1‑94 (MQ=60)

                                     GAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCT                                                          <  SRR4302543.2423228/94‑1 (MQ=60)

                                       AGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCT                                                        <  SRR4302543.595977/94‑1 (MQ=60)

                                         CCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTT                                                      <  SRR4302543.1627339/94‑1 (MQ=60)

                                         CCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTT                                                      <  SRR4302543.812748/94‑1 (MQ=60)

                                         CCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTT                                                      <  SRR4302543.1084748/94‑1 (MQ=60)

                                          CTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTT                                                     <  SRR4302543.2066515/94‑1 (MQ=60)

                                          CTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTT                                                     >  SRR4302543.4715931/1‑94 (MQ=60)

                                           TGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTA                                                    <  SRR4302543.1087445/94‑1 (MQ=60)

                                            GGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAA                                                   >  SRR4302543.1092311/1‑94 (MQ=60)

                                             GGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAA                                                  <  SRR4302543.7866390/94‑1 (MQ=60)

                                              GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 <  SRR4302543.981700/94‑1 (MQ=60)

                                              GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 <  SRR4302543.6847352/94‑1 (MQ=60)

                                              GACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAAT                                                 <  SRR4302543.1152909/94‑1 (MQ=60)

                                               ACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATC                                                >  SRR4302543.607053/1‑94 (MQ=60)

                                                CGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCC                                               >  SRR4302543.7202982/1‑94 (MQ=60)

                                                CGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCC                                               >  SRR4302543.5885749/1‑94 (MQ=60)

                                                 GGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCT                                              <  SRR4302543.5633919/94‑1 (MQ=60)

                                                  GTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTG                                             >  SRR4302543.5225486/1‑94 (MQ=60)

                                                   TCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGC                                            <  SRR4302543.230986/94‑1 (MQ=60)

                                                    CCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCT                                           <  SRR4302543.7137403/94‑1 (MQ=60)

                                                     CGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTT                                          <  SRR4302543.1482035/94‑1 (MQ=60)

                                                     CGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTT                                          >  SRR4302543.7236398/1‑94 (MQ=60)

                                                      GCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTA                                         >  SRR4302543.1919025/1‑94 (MQ=60)

                                                      GCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTA                                         >  SRR4302543.2119215/1‑94 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        <  SRR4302543.4094215/94‑1 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        >  SRR4302543.2701223/1‑94 (MQ=60)

                                                       CTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTAC                                        >  SRR4302543.2469299/1‑94 (MQ=60)

                                                        TCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACT                                       >  SRR4302543.7020893/1‑94 (MQ=60)

                                                         CTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTG                                      <  SRR4302543.4287743/94‑1 (MQ=60)

                                                         CTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCCGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTA                                      >  SRR4302543.5565095/1‑94 (MQ=60)

                                                          TCCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGA                                     >  SRR4302543.1244234/1‑94 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    >  SRR4302543.5845885/1‑94 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    <  SRR4302543.94264/94‑1 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    >  SRR4302543.6550361/1‑94 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    >  SRR4302543.5941020/1‑94 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    >  SRR4302543.7289405/1‑94 (MQ=60)

                                                           CCTTAAACGGTAAACGTATCGCCACCTCTTATCACCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAA                                    >  SRR4302543.6184228/1‑94 (MQ=60)

                                                            CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   <  SRR4302543.5301807/94‑1 (MQ=60)

                                                            CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   >  SRR4302543.660494/1‑94 (MQ=60)

                                                            CTTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAAC                                   <  SRR4302543.6506217/94‑1 (MQ=60)

                                                             TTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACG                                  >  SRR4302543.5606836/1‑94 (MQ=60)

                                                             TTAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACG                                  >  SRR4302543.1264491/1‑94 (MQ=60)

                                                              TAAACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGG                                 <  SRR4302543.1899639/94‑1 (MQ=60)

                                                                AACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTT                               >  SRR4302543.6910518/1‑94 (MQ=60)

                                                                AACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTT                               <  SRR4302543.225134/94‑1 (MQ=60)

                                                                AACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTT                               <  SRR4302543.2226731/94‑1 (MQ=60)

                                                                 ACGGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTC                              <  SRR4302543.6750873/94‑1 (MQ=60)

                                                                  acGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCT                             <  SRR4302543.592817/92‑1 (MQ=60)

                                                                   GGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTG                            <  SRR4302543.7684473/94‑1 (MQ=60)

                                                                   GGTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTG                            >  SRR4302543.3816825/1‑94 (MQ=60)

                                                                    GTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGT                           >  SRR4302543.7730768/1‑94 (MQ=60)

                                                                    GTAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGT                           >  SRR4302543.3115957/1‑94 (MQ=60)

                                                                     TAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTT                          <  SRR4302543.3657993/94‑1 (MQ=60)

                                                                     TAAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTT                          <  SRR4302543.6910956/94‑1 (MQ=60)

                                                                      AAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTG                         >  SRR4302543.6362990/1‑94 (MQ=60)

                                                                      AAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTG                         >  SRR4302543.657813/1‑94 (MQ=60)

                                                                      AAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTG                         >  SRR4302543.1219611/1‑94 (MQ=60)

                                                                      AAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTG                         <  SRR4302543.2567547/94‑1 (MQ=60)

                                                                      AAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTG                         >  SRR4302543.4740458/1‑94 (MQ=60)

                                                                      AAACGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTG                         <  SRR4302543.7611461/94‑1 (MQ=60)

                                                                         CGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAG                      >  SRR4302543.5918128/1‑94 (MQ=60)

                                                                         CGTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAG                      <  SRR4302543.1697649/94‑1 (MQ=60)

                                                                          GTATCGCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGT                     >  SRR4302543.5083258/1‑94 (MQ=60)

                                                                               GCCACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCC                >  SRR4302543.2404206/1‑94 (MQ=60)

                                                                                 CACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCG              <  SRR4302543.1693853/94‑1 (MQ=60)

                                                                                  ACCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGC             >  SRR4302543.3983551/1‑94 (MQ=60)

                                                                                   CCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCG            <  SRR4302543.3971042/94‑1 (MQ=60)

                                                                                   CCTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCG            >  SRR4302543.2783447/1‑94 (MQ=60)

                                                                                    CTCTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGT           <  SRR4302543.4623210/94‑1 (MQ=60)

                                                                                      CTTATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGC         >  SRR4302543.6891261/1‑94 (MQ=60)

                                                                                        TATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCG       >  SRR4302543.5997311/1‑94 (MQ=60)

                                                                                         ATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG      >  SRR4302543.4345326/1‑94 (MQ=60)

                                                                                         ATCCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGG      >  SRR4302543.3940947/1‑94 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    <  SRR4302543.5694920/94‑1 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    >  SRR4302543.3319490/1‑94 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    >  SRR4302543.1298281/1‑94 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    <  SRR4302543.505494/94‑1 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    <  SRR4302543.6047560/94‑1 (MQ=60)

                                                                                           CCCCACCTGCTCAAGCGTGATCTCGACCAGAAAGGCATCTCTTTTAAATCATGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGAC    <  SRR4302543.6047559/94‑1 (MQ=60)

                                                                                             CCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  SRR4302543.5083032/1‑94 (MQ=60)

                                                                                             CCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  SRR4302543.4125994/1‑94 (MQ=60)

CGGCGGCTGTCGTCTTTCGCTGGCAACGCCGGTTGATGAAGCCTGGGACGGTCCGCTCTCCTTAAACGGTAAACGTATCGCCACCTCTTATCCTCACCTGCTCAAGCGTTATCTCGACCAGAAAGGCATCTCTTTTAAATCCTGCTTACTGAACGGTTCTGTTGAAGTCGCCCCGCGTGCCGGACTG  >  NC_000913/2088516‑2088702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`