Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,094,539 T→C V225A (GTT→GCT)  hisF → imidazole glycerol phosphate synthase holoenzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,094,5390TC100.0% 646.0 / NA 206V225A (GTT→GCT) hisFimidazole glycerol phosphate synthase holoenzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/108);  total (98/108)

Only 100 of 216 total aligned reads displayed.

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628
                                                                                          |                                                                                         
aaGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTg                                                                                         >  2:6066845/1‑93 (MQ=255)
 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  1:3722356/93‑1 (MQ=255)
 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  2:3757814/93‑1 (MQ=255)
  gTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAc                                                                                       >  2:4994709/1‑93 (MQ=255)
      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  1:3468148/1‑93 (MQ=255)
        gAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcg                                                                                 >  1:3900746/1‑93 (MQ=255)
           gTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCCCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTg                                                                              <  2:7306066/93‑1 (MQ=255)
             ttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGc                                                                            <  1:3023712/93‑1 (MQ=255)
             ttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGc                                                                            <  2:281120/93‑1 (MQ=255)
              tGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCa                                                                           <  2:2655106/93‑1 (MQ=255)
                ccACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGc                                                                         >  1:6277468/1‑93 (MQ=255)
                 cACGTCCCGCTGTTTGCCTCCGGTGGCGCGGGCACCATGGACCACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:5139625/1‑93 (MQ=255)
                 cACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:6833600/1‑93 (MQ=255)
                 cACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:5851871/1‑93 (MQ=255)
                 cACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:7817925/1‑93 (MQ=255)
                  aCGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCtt                                                                       >  1:6565624/1‑93 (MQ=255)
                  aCGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCACCtt                                                                       >  1:6752553/1‑93 (MQ=255)
                   cGTCTCGCTGATTGCCGCCGGTGGCGCGGGCAGCATGGAACACTGCCTCTAAACCTTCCGCGATGCCGACGCTGACGGCGCGTTGGCAGCTTc                                                                      <  2:6185503/93‑1 (MQ=255)
                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      >  1:642738/1‑93 (MQ=255)
                    gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     >  1:2922473/1‑93 (MQ=255)
                    gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     >  1:3762678/1‑93 (MQ=255)
                    gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     >  1:7202789/1‑93 (MQ=255)
                    gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     >  1:6936759/1‑93 (MQ=255)
                    gTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     <  1:5906326/93‑1 (MQ=255)
                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  2:251030/1‑93 (MQ=255)
                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  1:685857/1‑93 (MQ=255)
                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  <  2:5622916/93‑1 (MQ=255)
                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  <  2:7412030/93‑1 (MQ=255)
                        cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 <  1:982690/93‑1 (MQ=255)
                        cGCTGATTGCCTCCGGTAGCGAGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCCATGCCGACGCTGACGGTGCGCTGGCAGCTTCCGTAt                                                                 >  2:3976870/1‑93 (MQ=255)
                         gCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTTCGTAtt                                                                <  2:7680003/93‑1 (MQ=255)
                         gCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAtt                                                                <  1:2516053/93‑1 (MQ=255)
                         gCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGAGCGCTAGCAGCTTCCGTAtc                                                                >  2:3173819/1‑92 (MQ=255)
                          cTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTc                                                               >  1:5566996/1‑93 (MQ=255)
                           tGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTcc                                                              <  2:6343551/93‑1 (MQ=255)
                            gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             <  1:3428366/93‑1 (MQ=255)
                            gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             <  2:1890330/93‑1 (MQ=255)
                             aTTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            <  2:3474770/93‑1 (MQ=255)
                             aTTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            >  1:4408273/1‑93 (MQ=255)
                              ttGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcaca                                                           <  1:4257066/93‑1 (MQ=255)
                                gCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCAGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaa                                                         >  1:1364171/1‑93 (MQ=255)
                                 ccTCCCGTGGCCCCGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaac                                                        <  1:5492954/93‑1 (MQ=255)
                                   tCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaacaa                                                      >  1:7748796/1‑93 (MQ=255)
                                   tCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaacaa                                                      <  1:2632391/93‑1 (MQ=255)
                                    ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  2:1824536/93‑1 (MQ=255)
                                    ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  1:5783341/93‑1 (MQ=255)
                                    ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  2:7708540/93‑1 (MQ=255)
                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:2359932/93‑1 (MQ=255)
                                     cGGGGGCGCGGGCACCATGGAACACTTCCGCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:5614904/93‑1 (MQ=255)
                                      ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   <  1:480783/93‑1 (MQ=255)
                                      ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   <  1:1801116/93‑1 (MQ=255)
                                        tGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaataat                                                 <  1:5171392/93‑1 (MQ=255)
                                        tGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaataat                                                 >  1:1624702/1‑93 (MQ=255)
                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  2:6813978/93‑1 (MQ=255)
                                              gggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAatat                                           <  1:1239810/93‑1 (MQ=255)
                                              gggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAatat                                           >  1:2164840/1‑93 (MQ=255)
                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          >  1:2938429/1‑93 (MQ=255)
                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          >  2:577886/1‑93 (MQ=255)
                                               gccACCATGGAACACTTCCACGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          <  2:4329560/91‑1 (MQ=255)
                                                gCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTg                                         <  2:5985545/93‑1 (MQ=255)
                                                gCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTg                                         <  1:582801/93‑1 (MQ=255)
                                                   ccATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTg                                      <  2:5569906/93‑1 (MQ=255)
                                                   ccATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTg                                      >  1:5271750/1‑93 (MQ=255)
                                                    cATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGa                                     <  1:1835950/93‑1 (MQ=255)
                                                     aTGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGaa                                    >  2:5132879/1‑93 (MQ=255)
                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   >  2:6562708/1‑93 (MQ=255)
                                                         aaCCCTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaa                                <  2:4389417/93‑1 (MQ=255)
                                                         aaCACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaa                                <  1:149833/93‑1 (MQ=255)
                                                         aaCACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaa                                <  1:2249028/93‑1 (MQ=255)
                                                          acacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaa                               >  2:2555892/1‑93 (MQ=255)
                                                               tCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGt                          >  2:2901015/1‑93 (MQ=255)
                                                               tCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGt                          <  2:6768678/93‑1 (MQ=255)
                                                                  tCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAcc                       <  1:312887/93‑1 (MQ=255)
                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAccg                      >  2:4161042/1‑92 (MQ=255)
                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      >  2:7764523/1‑93 (MQ=255)
                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  1:791496/93‑1 (MQ=255)
                                                                    gAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTg                     >  2:4435975/1‑93 (MQ=255)
                                                                      aGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGc                   >  2:623516/1‑93 (MQ=255)
                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  2:5366783/1‑93 (MQ=255)
                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  2:2522078/1‑93 (MQ=255)
                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGAGTACCTGGCa                  >  2:2822350/1‑93 (MQ=255)
                                                                        ccTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCaa                 >  2:83022/1‑93 (MQ=255)
                                                                         cTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAac                >  2:695943/1‑93 (MQ=255)
                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               >  2:198611/1‑93 (MQ=255)
                                                                           tCCGCGATGCCGACGCTGAAGGCGGGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAacac              <  1:2043779/93‑1 (MQ=255)
                                                                            ccGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAcaca             <  1:1070820/93‑1 (MQ=255)
                                                                            ccGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAcaca             >  2:1203470/1‑93 (MQ=255)
                                                                             cgcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAg            <  2:125471/93‑1 (MQ=255)
                                                                             cgcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAg            <  1:6340826/93‑1 (MQ=255)
                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           >  1:4002472/1‑93 (MQ=255)
                                                                                 aTGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCg        >  2:612439/1‑93 (MQ=255)
                                                                                 aTGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCg        >  1:7566854/1‑93 (MQ=255)
                                                                                   gCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTg      <  1:5398435/93‑1 (MQ=255)
                                                                                    ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     >  2:5239139/1‑93 (MQ=255)
                                                                                     cGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGga    <  2:6042709/93‑1 (MQ=255)
                                                                                     cGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGga    >  1:3642376/1‑93 (MQ=255)
                                                                                     cGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGga    >  2:1159734/1‑93 (MQ=255)
                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  1:623107/93‑1 (MQ=255)
                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  >  2:7575327/1‑93 (MQ=255)
                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  1:6614805/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCA  >  NC_000913/2094446‑2094631
                                                                                             |                                                                                            
AAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGC                                                                                              >  SRR4302543.3504082/1‑94 (MQ=60)
 AAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCT                                                                                             >  SRR4302543.347418/1‑94 (MQ=60)
    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                          <  SRR4302543.3755092/94‑1 (MQ=60)
    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                          >  SRR4302543.1332347/1‑94 (MQ=60)
    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                          >  SRR4302543.7836018/1‑94 (MQ=60)
    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                          <  SRR4302543.5550944/94‑1 (MQ=60)
    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                          <  SRR4302543.3755091/94‑1 (MQ=60)
      TGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGG                                                                                        >  SRR4302543.2297724/1‑94 (MQ=60)
       GCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGC                                                                                       <  SRR4302543.3461831/94‑1 (MQ=60)
       GCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGC                                                                                       <  SRR4302543.5855156/94‑1 (MQ=60)
        CGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCG                                                                                      <  SRR4302543.7046550/94‑1 (MQ=60)
        CGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCG                                                                                      >  SRR4302543.3499211/1‑94 (MQ=60)
         GTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGC                                                                                     >  SRR4302543.7752752/1‑94 (MQ=60)
          TGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCG                                                                                    >  SRR4302543.3935191/1‑94 (MQ=60)
          TGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCG                                                                                    >  SRR4302543.2934489/1‑94 (MQ=60)
               TTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGC                                                                               >  SRR4302543.6678148/1‑94 (MQ=60)
                TTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCA                                                                              <  SRR4302543.3051042/94‑1 (MQ=60)
                 TGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAG                                                                             >  SRR4302543.6412244/1‑94 (MQ=60)
                  GCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGC                                                                            >  SRR4302543.6336644/1‑94 (MQ=60)
                   CCACGTCCCGCTGTTTGCCTCCGGTGGCGCGGGCACCATGGACCACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                           >  SRR4302543.5186087/1‑94 (MQ=60)
                   CCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                           >  SRR4302543.7891584/1‑94 (MQ=60)
                   CCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                           >  SRR4302543.5186088/1‑94 (MQ=60)
                   CCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                           >  SRR4302543.5905119/1‑94 (MQ=60)
                   CCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                           >  SRR4302543.6898113/1‑94 (MQ=60)
                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTT                                                                          >  SRR4302543.6627403/1‑94 (MQ=60)
                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCACCTT                                                                          >  SRR4302543.6816324/1‑94 (MQ=60)
                     ACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTC                                                                         >  SRR4302543.4399234/1‑94 (MQ=60)
                     ACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTC                                                                         >  SRR4302543.648608/1‑94 (MQ=60)
                      cgtgcccgtggtttgccccggtgggcggggccCCATGGAACCCCTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        <  SRR4302543.5973576/62‑1 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        >  SRR4302543.7272621/1‑94 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        >  SRR4302543.3795796/1‑94 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        <  SRR4302543.5626307/94‑1 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        >  SRR4302543.7002011/1‑94 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        >  SRR4302543.2949023/1‑94 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCACGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                        <  SRR4302543.6920767/94‑1 (MQ=60)
                      CGTCCCGCTGATTGCCTCCGGAGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCG                                                                        >  SRR4302543.2528514/1‑94 (MQ=60)
                       GTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                       >  SRR4302543.570515/1‑94 (MQ=60)
                       GTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                       <  SRR4302543.3151584/94‑1 (MQ=60)
                       GTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                       <  SRR4302543.5960231/94‑1 (MQ=60)
                       GTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                       >  SRR4302543.692173/1‑94 (MQ=60)
                           CGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATT                                                                   <  SRR4302543.991361/94‑1 (MQ=60)
                           CGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATT                                                                   <  SRR4302543.3609850/94‑1 (MQ=60)
                           CGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATT                                                                   >  SRR4302543.3242485/1‑94 (MQ=60)
                           CGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATT                                                                   <  SRR4302543.6662985/94‑1 (MQ=60)
                           CGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATC                                                                   >  SRR4302543.1823796/1‑94 (MQ=60)
                            GCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTC                                                                  <  SRR4302543.2538908/94‑1 (MQ=60)
                            GCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTC                                                                  >  SRR4302543.5617645/1‑94 (MQ=60)
                              TGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCA                                                                >  SRR4302543.958934/1‑94 (MQ=60)
                              TGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCA                                                                <  SRR4302543.2147195/94‑1 (MQ=60)
                              TGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCA                                                                <  SRR4302543.2060575/94‑1 (MQ=60)
                               GATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAC                                                               >  SRR4302543.7959128/1‑94 (MQ=60)
                               GATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAC                                                               <  SRR4302543.3459044/94‑1 (MQ=60)
                               GATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAC                                                               >  SRR4302543.4448230/1‑94 (MQ=60)
                                 TTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAA                                                             <  SRR4302543.4295507/94‑1 (MQ=60)
                                 TTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAA                                                             <  SRR4302543.4295716/94‑1 (MQ=60)
                                  TGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCAGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAA                                                            >  SRR4302543.1376396/1‑94 (MQ=60)
                                   GCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAAC                                                           <  SRR4302543.2263710/94‑1 (MQ=60)
                                    gctccggtggcggttGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACA                                                          <  SRR4302543.5542773/79‑1 (MQ=60)
                                    CCTCCCGTGGCCCCGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACA                                                          <  SRR4302543.5542771/94‑1 (MQ=60)
                                     CTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAA                                                         >  SRR4302543.7821972/1‑94 (MQ=60)
                                     CTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAA                                                         >  SRR4302543.5415323/1‑94 (MQ=60)
                                      TCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAA                                                        <  SRR4302543.2656224/94‑1 (MQ=60)
                                       CCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAAT                                                       <  SRR4302543.5835965/94‑1 (MQ=60)
                                        CGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                      <  SRR4302543.2381334/94‑1 (MQ=60)
                                        CGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                      <  SRR4302543.2830593/94‑1 (MQ=60)
                                        CGGTGGCGCGGGCAACATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                      <  SRR4302543.696356/94‑1 (MQ=60)
                                        CGGGGGCGCGGGCACCATGGAACACTTCCGCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                      <  SRR4302543.5666071/94‑1 (MQ=60)
                                         GGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAA                                                     <  SRR4302543.485226/94‑1 (MQ=60)
                                         GGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAA                                                     <  SRR4302543.1817243/94‑1 (MQ=60)
                                         GGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAA                                                     >  SRR4302543.3784623/1‑94 (MQ=60)
                                         GGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAA                                                     >  SRR4302543.4876043/1‑94 (MQ=60)
                                          GTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAAT                                                    >  SRR4302543.1639180/1‑94 (MQ=60)
                                          GTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAAT                                                    <  SRR4302543.3874598/94‑1 (MQ=60)
                                           TGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATC                                                   <  SRR4302543.5218099/94‑1 (MQ=60)
                                            GGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCA                                                  <  SRR4302543.6217793/94‑1 (MQ=60)
                                                CGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAT                                              >  SRR4302543.2184237/1‑94 (MQ=60)
                                                 GGGCTCCCTGGAACACTTCCTCGAAGACTTCCGCGATGCCGACGATGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATT                                             <  SRR4302543.5304413/94‑1 (MQ=60)
                                                 GGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATT                                             <  SRR4302543.1250952/94‑1 (MQ=60)
                                                 GGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATT                                             >  SRR4302543.2965116/1‑94 (MQ=60)
                                                 GGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATT                                             <  SRR4302543.4347760/94‑1 (MQ=60)
                                                   GCCCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGG                                           <  SRR4302543.5955158/94‑1 (MQ=60)
                                                   GCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGG                                           <  SRR4302543.588124/94‑1 (MQ=60)
                                                   GCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGG                                           >  SRR4302543.2407385/1‑94 (MQ=60)
                                                     ACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTG                                         >  SRR4302543.5319309/1‑94 (MQ=60)
                                                       CATGGAACACTTCCTGGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAA                                       <  SRR4302543.1546613/94‑1 (MQ=60)
                                                       CATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAA                                       <  SRR4302543.1852477/94‑1 (MQ=60)
                                                       CATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAA                                       >  SRR4302543.2609855/1‑94 (MQ=60)
                                                          GGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTA                                    >  SRR4302543.6314666/1‑94 (MQ=60)
                                                           GAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAA                                   <  SRR4302543.744393/94‑1 (MQ=60)
                                                            AACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAA                                  <  SRR4302543.2269452/94‑1 (MQ=60)
                                                            AACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAA                                  <  SRR4302543.151134/94‑1 (MQ=60)
                                                              CACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAG                                <  SRR4302543.6147500/94‑1 (MQ=60)
                                                               ACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGC                               >  SRR4302543.1484768/1‑94 (MQ=60)
                                                               ACTGCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGC                               <  SRR4302543.5514653/94‑1 (MQ=60)
                                                                 TTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGT                             >  SRR4302543.5897551/1‑94 (MQ=60)
                                                                    CTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACC                          >  SRR4302543.3995814/1‑94 (MQ=60)
                                                                     TCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCT                         <  SRR4302543.315710/94‑1 (MQ=60)
                                                                     TCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCT                         <  SRR4302543.2960798/94‑1 (MQ=60)
                                                                      CGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                        <  SRR4302543.798454/94‑1 (MQ=60)
                                                                      CGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                        <  SRR4302543.7670490/94‑1 (MQ=60)
                                                                       GAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGG                       >  SRR4302543.1155450/1‑94 (MQ=60)
                                                                          GCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAA                    <  SRR4302543.360370/94‑1 (MQ=60)
                                                                             TTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACAC                 <  SRR4302543.3042268/94‑1 (MQ=60)
                                                                              TCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACA                <  SRR4302543.6836030/94‑1 (MQ=60)
                                                                              TCCGCGATGCCGACGCTGAAGGCGGGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACA                <  SRR4302543.2062107/94‑1 (MQ=60)
                                                                               CCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAG               <  SRR4302543.1080445/94‑1 (MQ=60)
                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG              <  SRR4302543.6400446/94‑1 (MQ=60)
                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG              <  SRR4302543.4682630/94‑1 (MQ=60)
                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG              >  SRR4302543.4038176/1‑94 (MQ=60)
                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG              <  SRR4302543.6400447/94‑1 (MQ=60)
                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG              <  SRR4302543.1705779/94‑1 (MQ=60)
                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG              <  SRR4302543.6805036/94‑1 (MQ=60)
                                                                                   GATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCG           >  SRR4302543.7638857/1‑94 (MQ=60)
                                                                                    ATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGG          >  SRR4302543.6500826/1‑94 (MQ=60)
                                                                                      GCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGG        <  SRR4302543.5447530/94‑1 (MQ=60)
                                                                                       CCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGA       >  SRR4302543.3674415/1‑94 (MQ=60)
                                                                                       CCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGA       >  SRR4302543.1880340/1‑94 (MQ=60)
                                                                                          ACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGAT    <  SRR4302543.628813/94‑1 (MQ=60)
                                                                                          ACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGAT    <  SRR4302543.6677171/94‑1 (MQ=60)
                                                                                           CGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATC   >  SRR4302543.245904/1‑94 (MQ=60)
                                                                                            GCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCA  <  SRR4302543.2515393/94‑1 (MQ=60)
                                                                                             |                                                                                            
AAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCA  >  NC_000913/2094446‑2094631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑