Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,097,074 T→C T188A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,097,0740TC99.2% 788.1 / ‑5.2 251T188A (ACC→GCC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (142/107);  total (143/108)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096985‑2097163
                                                                                         |                                                                                         
aTAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaaa                                                                                        >  2:7768507/1‑93 (MQ=255)
  aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAACGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      <  2:3744557/93‑1 (MQ=255)
  aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      <  1:4150864/93‑1 (MQ=255)
   gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     <  1:3793725/93‑1 (MQ=255)
    cTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGc                                                                                    <  1:4506411/93‑1 (MQ=255)
     tagccAGTTCGTTAAAGGACGCCACGCGCATCGCCAGGTAGGTGTATGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGTGGCATACAGCa                                                                                   >  2:7027207/4‑93 (MQ=255)
     tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   >  2:1662274/1‑93 (MQ=255)
     tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  2:5868870/93‑1 (MQ=255)
      aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  <  1:3654419/93‑1 (MQ=255)
      aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  1:2866682/1‑93 (MQ=255)
      aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  1:5397622/1‑93 (MQ=255)
         cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAACAGCATCgg                                                                               >  2:2947241/1‑93 (MQ=255)
           gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  2:4074975/1‑93 (MQ=255)
           gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             <  1:3984728/93‑1 (MQ=255)
            ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            <  2:4383473/93‑1 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        <  1:6060335/93‑1 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        >  1:856031/1‑93 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        >  2:85830/1‑93 (MQ=255)
                 tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtttt                                                                       <  2:5712562/93‑1 (MQ=255)
                   aaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGc                                                                     <  1:30675/93‑1 (MQ=255)
                    aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  2:2056514/93‑1 (MQ=255)
                    aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  2:2635926/93‑1 (MQ=255)
                     gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  2:405027/93‑1 (MQ=255)
                        cGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAt                                                                >  1:957665/1‑93 (MQ=255)
                         gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               >  1:1847068/1‑93 (MQ=255)
                         gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  1:7350613/93‑1 (MQ=255)
                          ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              <  2:5607651/93‑1 (MQ=255)
                          ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              <  2:4546522/93‑1 (MQ=255)
                           cACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgc                                                             >  1:7783123/1‑93 (MQ=255)
                            aCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgcg                                                            >  1:2067851/1‑93 (MQ=255)
                            aCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgcg                                                            >  1:5854928/1‑93 (MQ=255)
                             cgcgCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCgcgc                                                           >  1:382361/1‑93 (MQ=255)
                              gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          <  1:1080752/93‑1 (MQ=255)
                              gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          <  2:4708685/93‑1 (MQ=255)
                              gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          <  1:4709352/93‑1 (MQ=255)
                               cgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCt                                                         >  2:254264/1‑93 (MQ=255)
                                 cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTc                                                       >  2:7517693/1‑93 (MQ=255)
                                  aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  2:1232251/1‑93 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     >  2:7875050/1‑93 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     <  2:6898595/93‑1 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     <  1:3571966/93‑1 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     <  2:4319063/93‑1 (MQ=255)
                                       cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 <  1:6332399/93‑1 (MQ=255)
                                       cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 >  1:2311691/1‑93 (MQ=255)
                                       cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 <  1:3233803/93‑1 (MQ=255)
                                        aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCTCGCCTTCCTGTAAc                                                <  2:3676099/93‑1 (MQ=255)
                                        aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  1:5679448/1‑93 (MQ=255)
                                           taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             <  2:3211346/93‑1 (MQ=255)
                                           taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             >  2:7708760/1‑93 (MQ=255)
                                           taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             >  1:1266398/1‑93 (MQ=255)
                                           taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             <  2:5241554/93‑1 (MQ=255)
                                            aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            >  2:3895328/1‑93 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  1:6903828/93‑1 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  1:6570485/1‑93 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  2:2830905/93‑1 (MQ=255)
                                              gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          <  2:1075539/93‑1 (MQ=255)
                                              gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          <  2:7290004/93‑1 (MQ=255)
                                              gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          <  2:1417039/93‑1 (MQ=255)
                                              gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          >  1:7923332/1‑93 (MQ=255)
                                               tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  1:7268906/1‑93 (MQ=255)
                                                gtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcagc                                        >  2:6634773/1‑93 (MQ=255)
                                                gtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcagc                                        >  1:5932555/1‑93 (MQ=255)
                                                 tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  1:6446623/1‑93 (MQ=255)
                                                    gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  2:2171973/93‑1 (MQ=255)
                                                    gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    >  2:329345/1‑93 (MQ=255)
                                                        aaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGtt                                >  1:553280/1‑93 (MQ=255)
                                                        aaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGtt                                >  1:5982453/1‑93 (MQ=255)
                                                         aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               >  2:1315675/1‑93 (MQ=255)
                                                            tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            >  2:6296647/1‑93 (MQ=255)
                                                             ttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgcg                           >  2:1805339/1‑93 (MQ=255)
                                                                 tCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGtt                       <  2:5098380/93‑1 (MQ=255)
                                                                   gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     >  1:5028373/1‑93 (MQ=255)
                                                                    cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    <  1:6966004/93‑1 (MQ=255)
                                                                    cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    <  1:7689956/93‑1 (MQ=255)
                                                                    cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    >  2:1745723/1‑93 (MQ=255)
                                                                    cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    >  1:2642789/1‑93 (MQ=255)
                                                                     ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:1924033/1‑93 (MQ=255)
                                                                     ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:3107381/1‑93 (MQ=255)
                                                                     ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:6221961/1‑93 (MQ=255)
                                                                     ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  1:4911277/1‑93 (MQ=255)
                                                                     ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  1:3211526/1‑93 (MQ=255)
                                                                      tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  1:2223643/1‑93 (MQ=255)
                                                                      tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  2:4879616/1‑93 (MQ=255)
                                                                       cTGCTTCAGTGGAGTCGGCAAACTGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAc                 <  2:5402841/93‑1 (MQ=255)
                                                                        tGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACg                >  1:19635/1‑93 (MQ=255)
                                                                         gCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGc               <  1:1616300/93‑1 (MQ=255)
                                                                         gCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGc               >  2:1703266/1‑93 (MQ=255)
                                                                           ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             >  2:7595662/1‑93 (MQ=255)
                                                                           ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             >  1:1199834/1‑93 (MQ=255)
                                                                           ttCAGTGGAGTCGGCAAACAGCATCGGGATAATTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             <  2:785275/93‑1 (MQ=255)
                                                                            tCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCa            <  2:3582020/93‑1 (MQ=255)
                                                                             cAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAc           >  1:5422098/1‑93 (MQ=255)
                                                                             cAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAc           <  2:1411546/93‑1 (MQ=255)
                                                                              aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          >  1:6538524/1‑93 (MQ=255)
                                                                               gTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCg         >  1:169543/1‑93 (MQ=255)
                                                                                tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  1:2400667/93‑1 (MQ=255)
                                                                                    gTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGAc    <  1:3111548/93‑1 (MQ=255)
                                                                                     tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   >  1:729912/1‑93 (MQ=255)
                                                                                      cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  <  2:6515352/93‑1 (MQ=255)
                                                                                      cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  >  1:3743285/1‑93 (MQ=255)
                                                                                         |                                                                                         
ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096985‑2097163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302543.5572929/1‑94 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302543.2603386/94‑1 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302543.313313/94‑1 (MQ=60)
      TGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAG                                                                                         <  SRR4302543.241821/94‑1 (MQ=60)
      AGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAG                                                                                         <  SRR4302543.4188390/94‑1 (MQ=60)
       GCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                        >  SRR4302543.3810828/1‑94 (MQ=60)
       GCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                        <  SRR4302543.3827150/94‑1 (MQ=60)
       GCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                        <  SRR4302543.1997966/94‑1 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       <  SRR4302543.2702970/94‑1 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       <  SRR4302543.4547388/94‑1 (MQ=60)
        CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGGAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                       >  SRR4302543.793651/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      <  SRR4302543.3259406/94‑1 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      >  SRR4302543.2892806/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      >  SRR4302543.5446708/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      >  SRR4302543.1261115/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302543.6531808/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     <  SRR4302543.3686519/94‑1 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302543.3758655/1‑94 (MQ=60)
           TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                    <  SRR4302543.2180278/94‑1 (MQ=60)
            CCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGG                                                                                   >  SRR4302543.832198/1‑94 (MQ=60)
             CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                  >  SRR4302543.3985521/1‑94 (MQ=60)
              AGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGA                                                                                 >  SRR4302543.7411812/1‑94 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                <  SRR4302543.4020088/94‑1 (MQ=60)
                TTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATA                                                                               >  SRR4302543.958877/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATT                                                                             >  SRR4302543.5516888/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             >  SRR4302543.3555735/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             >  SRR4302543.7325153/1‑94 (MQ=60)
                   GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                            >  SRR4302543.1464341/1‑94 (MQ=60)
                   GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                            >  SRR4302543.863653/1‑94 (MQ=60)
                    TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                           <  SRR4302543.6117709/94‑1 (MQ=60)
                    TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                           <  SRR4302543.2533283/94‑1 (MQ=60)
                     TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                          <  SRR4302543.5370649/94‑1 (MQ=60)
                     TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                          <  SRR4302543.7771288/94‑1 (MQ=60)
                       AAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCT                                                                        <  SRR4302543.30945/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      >  SRR4302543.7211144/1‑94 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      >  SRR4302543.7384238/1‑94 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      >  SRR4302543.1200101/1‑94 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    >  SRR4302543.966057/1‑94 (MQ=60)
                           ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                    <  SRR4302543.1941062/94‑1 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   >  SRR4302543.1257286/1‑94 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   >  SRR4302543.1863682/1‑94 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   >  SRR4302543.4130076/1‑94 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  <  SRR4302543.7421319/94‑1 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 >  SRR4302543.7856505/1‑94 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 >  SRR4302543.600441/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                <  SRR4302543.1715951/94‑1 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302543.5908223/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302543.5908220/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302543.2086338/1‑94 (MQ=60)
                                ACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGC                                                               >  SRR4302543.385893/1‑94 (MQ=60)
                                 CGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCC                                                              <  SRR4302543.429096/94‑1 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             <  SRR4302543.1090469/94‑1 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             <  SRR4302543.4752395/94‑1 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             <  SRR4302543.2335521/94‑1 (MQ=60)
                                    GCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTC                                                           >  SRR4302543.5385893/1‑94 (MQ=60)
                                       TCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTG                                                        <  SRR4302543.3603547/94‑1 (MQ=60)
                                          CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     >  SRR4302543.849294/1‑94 (MQ=60)
                                          CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     >  SRR4302543.2332726/1‑94 (MQ=60)
                                          CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     >  SRR4302543.2332725/1‑94 (MQ=60)
                                          CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     <  SRR4302543.5810906/94‑1 (MQ=60)
                                           CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                    >  SRR4302543.5731292/1‑94 (MQ=60)
                                           CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                    <  SRR4302543.3263055/94‑1 (MQ=60)
                                           CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                    >  SRR4302543.6304945/1‑94 (MQ=60)
                                           CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                    <  SRR4302543.6391952/94‑1 (MQ=60)
                                            AGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACA                                                   >  SRR4302543.3856698/1‑94 (MQ=60)
                                            AGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACA                                                   >  SRR4302543.1912219/1‑94 (MQ=60)
                                             GGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  >  SRR4302543.6471890/1‑94 (MQ=60)
                                              GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                 >  SRR4302543.1277729/1‑94 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302543.6632341/1‑94 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               <  SRR4302543.1683494/94‑1 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              <  SRR4302543.6623442/94‑1 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              <  SRR4302543.6968702/94‑1 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              >  SRR4302543.7997751/1‑94 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             >  SRR4302543.7339133/1‑94 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             >  SRR4302543.7339134/1‑94 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             >  SRR4302543.7664159/1‑94 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGCCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             >  SRR4302543.3141575/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302543.6450493/1‑94 (MQ=60)
                                                   TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                            >  SRR4302543.5986813/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302543.6507359/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302543.5679234/1‑94 (MQ=60)
                                                        GCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       >  SRR4302543.3160419/1‑94 (MQ=60)
                                                         CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                      >  SRR4302543.7763001/1‑94 (MQ=60)
                                                           AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                    >  SRR4302543.558365/1‑94 (MQ=60)
                                                           AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                    >  SRR4302543.4754896/1‑94 (MQ=60)
                                                           AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                    >  SRR4302543.6037268/1‑94 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTACTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  >  SRR4302543.6691020/1‑94 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 <  SRR4302543.2965551/94‑1 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                >  SRR4302543.4882094/1‑94 (MQ=60)
                                                                 TTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGC                              <  SRR4302543.546325/94‑1 (MQ=60)
                                                                     TCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTC                          <  SRR4302543.3815024/94‑1 (MQ=60)
                                                                      CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                         >  SRR4302543.5074130/1‑94 (MQ=60)
                                                                      CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                         >  SRR4302543.3863035/1‑94 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        <  SRR4302543.4694502/94‑1 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        >  SRR4302543.6107532/1‑94 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        >  SRR4302543.2666725/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       <  SRR4302543.7031556/94‑1 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302543.4956040/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       <  SRR4302543.7762768/94‑1 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302543.3240528/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       >  SRR4302543.3692842/1‑94 (MQ=60)
                                                                        CGTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       <  SRR4302543.597327/94‑1 (MQ=60)
                                                                         TTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAA                      >  SRR4302543.2243715/1‑94 (MQ=60)
                                                                         TTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAA                      >  SRR4302543.3058020/1‑94 (MQ=60)
                                                                          TCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAC                     >  SRR4302543.3351460/1‑94 (MQ=60)
                                                                           CTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACG                    >  SRR4302543.6737527/1‑94 (MQ=60)
                                                                           CTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACG                    >  SRR4302543.19817/1‑94 (MQ=60)
                                                                           CTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACG                    >  SRR4302543.4443891/1‑94 (MQ=60)
                                                                             GCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCT                  <  SRR4302543.5041374/94‑1 (MQ=60)
                                                                             GCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCT                  <  SRR4302543.1630709/94‑1 (MQ=60)
                                                                              CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                 >  SRR4302543.1210636/1‑94 (MQ=60)
                                                                               TTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCA                >  SRR4302543.2111897/1‑94 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               >  SRR4302543.5471394/1‑94 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               >  SRR4302543.56061/1‑94 (MQ=60)
                                                                                 CAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACC              >  SRR4302543.6600076/1‑94 (MQ=60)
                                                                                 CAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACC              <  SRR4302543.168569/94‑1 (MQ=60)
                                                                                  AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCG             >  SRR4302543.170994/1‑94 (MQ=60)
                                                                                   GTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGA            >  SRR4302543.6626025/1‑94 (MQ=60)
                                                                                    TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT           <  SRR4302543.2422374/94‑1 (MQ=60)
                                                                                    TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT           <  SRR4302543.5510130/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302543.6989009/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302543.3620449/94‑1 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       <  SRR4302543.6076660/94‑1 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       >  SRR4302543.736460/1‑94 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       <  SRR4302543.3139540/94‑1 (MQ=60)
                                                                                         TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA      >  SRR4302543.3776214/1‑94 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATA    >  SRR4302543.1231915/1‑94 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATA    >  SRR4302543.6741240/1‑94 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATAACCATA    >  SRR4302543.6741242/1‑94 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGGAACAGAGCAGCGAAACGTTCTGAGCGTTCTGAACGCTCACCGATGACAATA    >  SRR4302543.6741241/1‑94 (MQ=60)
                                                                                            GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC   >  SRR4302543.5724400/1‑94 (MQ=60)
                                                                                             CAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  <  SRR4302543.7808414/94‑1 (MQ=60)
                                                                                             CAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  SRR4302543.5532793/1‑94 (MQ=60)
                                                                                             CAAACAGCATCGGGATATTTTGCTTAATCCCGCCTTCCTGTAACAGCGCCGCGAAACGTTCTGCGCGTTCTGAACGCTAACCGATCACAATACG  >  SRR4302543.5532792/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑