Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,109,166 A→G V278A (GTC→GCC)  rfbD ← dTDP‑6‑deoxy‑L‑mannose‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,109,1660AG99.6% 865.5 / ‑4.9 274V278A (GTC→GCC) rfbDdTDP‑6‑deoxy‑L‑mannose‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (140/133);  total (140/134)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 294 total aligned reads displayed.

TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT  >  NC_000913/2109076‑2109255
                                                                                          |                                                                                         
tCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCaggcaaggca                                                                                         <  1:7871103/93‑1 (MQ=255)
 cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAggcaaggcaa                                                                                        <  1:126681/93‑1 (MQ=255)
 cACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAggcaaggcaa                                                                                        <  2:7860161/93‑1 (MQ=255)
  aCGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGgcaaggcaag                                                                                       >  2:4230338/1‑93 (MQ=255)
    gAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAGGGCAAgcg                                                                                     <  2:3384500/93‑1 (MQ=255)
      aCAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCa                                                                                   <  2:5603365/93‑1 (MQ=255)
       cAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaa                                                                                  <  1:6975228/93‑1 (MQ=255)
       aaaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaa                                                                                  <  1:5984713/92‑1 (MQ=255)
        aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaaa                                                                                 >  1:2031742/1‑93 (MQ=255)
        aaGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCaaa                                                                                 >  1:2362361/1‑93 (MQ=255)
           aTGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGtt                                                                              <  2:3278959/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTTCTTGAGTCGTAATTAATTCATAGAGCATTCTTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  2:374325/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             <  1:2763472/93‑1 (MQ=255)
            tGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTc                                                                             >  2:5541139/1‑93 (MQ=255)
             gCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTct                                                                            >  1:2911602/1‑93 (MQ=255)
                aaaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgct                                                                         <  1:6886495/93‑1 (MQ=255)
                 aaaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTctgctg                                                                        <  1:2135680/93‑1 (MQ=255)
                  aaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGa                                                                       >  1:7394097/1‑93 (MQ=255)
                  aaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGa                                                                       >  1:2985054/1‑93 (MQ=255)
                  aaCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGa                                                                       >  2:2143436/1‑93 (MQ=255)
                   aCTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGaa                                                                      >  1:215959/1‑93 (MQ=255)
                    cTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGaaa                                                                     >  2:7305599/1‑93 (MQ=255)
                    cTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGaaa                                                                     <  1:606178/93‑1 (MQ=255)
                     tATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAt                                                                    <  1:6647981/93‑1 (MQ=255)
                     tATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAt                                                                    >  2:2945199/1‑93 (MQ=255)
                     tATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAt                                                                    >  2:6010769/1‑93 (MQ=255)
                     tATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAt                                                                    >  1:2595436/1‑93 (MQ=255)
                     tATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAt                                                                    >  1:3770217/1‑93 (MQ=255)
                      aTTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAtt                                                                   >  2:6915547/1‑93 (MQ=255)
                       ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAttt                                                                  <  2:827459/93‑1 (MQ=255)
                       ttAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAttt                                                                  <  2:4568099/93‑1 (MQ=255)
                        tAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAtttt                                                                 >  2:7111798/1‑93 (MQ=255)
                          aaTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTc                                                               >  1:5067533/1‑93 (MQ=255)
                            ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTg                                                             >  1:1146584/1‑93 (MQ=255)
                            ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTg                                                             >  2:392520/1‑93 (MQ=255)
                            ttGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTg                                                             >  2:5021806/1‑93 (MQ=255)
                             tGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGt                                                            <  1:2808424/93‑1 (MQ=255)
                               cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt                                                          <  2:22422/93‑1 (MQ=255)
                               cTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAt                                                          >  1:7449761/1‑93 (MQ=255)
                                tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt                                                         >  2:3130527/1‑93 (MQ=255)
                                tGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAtt                                                         >  1:7297919/1‑93 (MQ=255)
                                 gTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCATGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTa                                                        <  1:4021031/93‑1 (MQ=255)
                                  tagtagTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       <  1:860903/93‑1 (MQ=255)
                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       <  1:3482587/93‑1 (MQ=255)
                                  tAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTaa                                                       >  2:737274/1‑93 (MQ=255)
                                   aGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAg                                                      >  2:4650640/1‑93 (MQ=255)
                                    gTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAgg                                                     <  1:4930539/93‑1 (MQ=255)
                                     tCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                    >  2:5485396/1‑93 (MQ=255)
                                     tCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                    >  2:1570810/1‑93 (MQ=255)
                                     tCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGc                                                    >  1:3673740/1‑93 (MQ=255)
                                       gTAAATATTTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  <  2:6999929/93‑1 (MQ=255)
                                       gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAATGCgg                                                  >  2:7197174/1‑92 (MQ=255)
                                       gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  <  2:1674121/93‑1 (MQ=255)
                                       gTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCga                                                  <  2:1775842/93‑1 (MQ=255)
                                        tAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgag                                                 >  2:4969824/1‑93 (MQ=255)
                                         aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga                                                <  2:6403795/93‑1 (MQ=255)
                                         aaaTAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCgaga                                                >  2:5286618/1‑93 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               <  2:2590080/93‑1 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               <  2:4038023/93‑1 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               >  1:2428354/1‑93 (MQ=255)
                                          aataatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGagag                                               >  1:4753471/1‑93 (MQ=255)
                                            taatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGtt                                             >  1:7571344/1‑93 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGATa                                            >  1:1129392/1‑93 (MQ=255)
                                             aatTCATTGAGCATTCGTTTCACGCCAACCAGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTa                                            <  2:7732256/93‑1 (MQ=255)
                                                tCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgt                                         <  2:664422/93‑1 (MQ=255)
                                                tCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgt                                         <  1:3286348/93‑1 (MQ=255)
                                                 cATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAtgtg                                        <  2:6765259/93‑1 (MQ=255)
                                                  aTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTgg                                       >  1:953667/1‑93 (MQ=255)
                                                  aTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTgg                                       <  1:7183073/93‑1 (MQ=255)
                                                  aTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTgg                                       >  1:7355888/1‑93 (MQ=255)
                                                   ttGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGga                                      <  2:4386344/93‑1 (MQ=255)
                                                       gCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGgacgac                                  <  2:7785672/93‑1 (MQ=255)
                                                         aTTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGAcgacga                                <  1:538546/93‑1 (MQ=255)
                                                           tCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGc                              >  1:161106/1‑93 (MQ=255)
                                                             gTTTCACGCCAGCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTg                            >  2:4862978/1‑93 (MQ=255)
                                                             gTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTg                            >  2:3831879/1‑93 (MQ=255)
                                                              tttCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTgg                           <  1:7704238/93‑1 (MQ=255)
                                                                tCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgtg                         <  2:5705872/93‑1 (MQ=255)
                                                                 cACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGgtgt                        >  1:1766401/1‑93 (MQ=255)
                                                                    gCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAgg                     <  2:2389653/93‑1 (MQ=255)
                                                                     ccAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                    <  2:1406327/93‑1 (MQ=255)
                                                                     ccAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                    <  1:1908518/93‑1 (MQ=255)
                                                                     ccAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGa                    <  2:7492804/93‑1 (MQ=255)
                                                                        aCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAg                 <  1:3896326/93‑1 (MQ=255)
                                                                        aCCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAg                 >  1:4247583/1‑93 (MQ=255)
                                                                          cTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGc               >  1:1059260/1‑93 (MQ=255)
                                                                           tGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt              >  2:3588238/1‑93 (MQ=255)
                                                                           tGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCt              >  1:1475598/1‑93 (MQ=255)
                                                                            gCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtg             >  1:5299208/1‑93 (MQ=255)
                                                                            gCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtg             >  2:1846771/1‑93 (MQ=255)
                                                                             ccAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgt            <  1:4449253/93‑1 (MQ=255)
                                                                              cAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTCGGATAGGCtgtt           >  2:7707075/1‑93 (MQ=255)
                                                                              cAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgtt           <  1:6211949/93‑1 (MQ=255)
                                                                              cAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgtt           <  2:1958196/93‑1 (MQ=255)
                                                                                gTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCtgttgt         >  2:1265870/1‑93 (MQ=255)
                                                                                 tCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTgttgtt        >  2:144930/1‑93 (MQ=255)
                                                                                   aggcaaggcaAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTgg      >  2:2255560/1‑93 (MQ=255)
                                                                                      cAAGGCAAGCGCAAAGTTCTGCTTAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTAc   <  2:4297036/93‑1 (MQ=255)
                                                                                      cAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTAc   <  1:4647395/93‑1 (MQ=255)
                                                                                      cAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTAc   <  1:5659702/93‑1 (MQ=255)
                                                                                          |                                                                                         
TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT  >  NC_000913/2109076‑2109255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTG  >  NC_000913/2109075‑2109256
                                                                                           |                                                                                          
ATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCA                                                                                          >  SRR4302543.7393311/1‑94 (MQ=60)
ATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCA                                                                                          >  SRR4302543.2376249/1‑94 (MQ=60)
 TCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAA                                                                                         <  SRR4302543.7945214/94‑1 (MQ=60)
  CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                        <  SRR4302543.7385201/94‑1 (MQ=60)
  CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                        <  SRR4302543.6194171/94‑1 (MQ=60)
  CACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAG                                                                                        <  SRR4302543.127824/94‑1 (MQ=60)
   ACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGC                                                                                       <  SRR4302543.230343/94‑1 (MQ=60)
   ACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGC                                                                                       <  SRR4302543.4683252/94‑1 (MQ=60)
    CGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCG                                                                                      <  SRR4302543.3592361/94‑1 (MQ=60)
    CGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCG                                                                                      <  SRR4302543.7769873/94‑1 (MQ=60)
     TAACAATATGCAAAAACTATTATGTTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCATCCTGCCAGTCAGGCAAGACAAGCGC                                                                                     <  SRR4302543.4120646/94‑1 (MQ=60)
     GAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGC                                                                                     >  SRR4302543.1457643/1‑94 (MQ=60)
        CAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAA                                                                                  >  SRR4302543.2049996/1‑94 (MQ=60)
        CAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAA                                                                                  <  SRR4302543.7040872/94‑1 (MQ=60)
        CAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAA                                                                                  >  SRR4302543.2383783/1‑94 (MQ=60)
        CAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAA                                                                                  <  SRR4302543.5585534/94‑1 (MQ=60)
        AAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAA                                                                                  <  SRR4302543.6039550/94‑1 (MQ=60)
          AGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGTCAAGGCAAGCGCAAAGT                                                                                >  SRR4302543.6080695/1‑94 (MQ=60)
          AGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGT                                                                                >  SRR4302543.2434120/1‑94 (MQ=60)
           GATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTT                                                                               >  SRR4302543.5112459/1‑94 (MQ=60)
             TGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                             >  SRR4302543.2938061/1‑94 (MQ=60)
             TGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCT                                                                             <  SRR4302543.2788618/94‑1 (MQ=60)
              GCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTG                                                                            <  SRR4302543.3758230/94‑1 (MQ=60)
               CAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGC                                                                           >  SRR4302543.7258758/1‑94 (MQ=60)
               CAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGC                                                                           >  SRR4302543.2489284/1‑94 (MQ=60)
               CAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGC                                                                           >  SRR4302543.2489283/1‑94 (MQ=60)
                 AAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTG                                                                         >  SRR4302543.3639815/1‑94 (MQ=60)
                 AAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTG                                                                         >  SRR4302543.7125558/1‑94 (MQ=60)
                 AAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTG                                                                         <  SRR4302543.6951267/94‑1 (MQ=60)
                  AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                        <  SRR4302543.2154788/94‑1 (MQ=60)
                  AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                        >  SRR4302543.3012162/1‑94 (MQ=60)
                  AAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGA                                                                        >  SRR4302543.7465111/1‑94 (MQ=60)
                   AACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAA                                                                       >  SRR4302543.217814/1‑94 (MQ=60)
                   AACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAA                                                                       <  SRR4302543.7456215/94‑1 (MQ=60)
                     CTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAT                                                                     <  SRR4302543.7598876/94‑1 (MQ=60)
                     CTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAT                                                                     >  SRR4302543.2619019/1‑94 (MQ=60)
                     CTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAT                                                                     <  SRR4302543.611718/94‑1 (MQ=60)
                     CTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAT                                                                     <  SRR4302543.6494734/94‑1 (MQ=60)
                     CTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAT                                                                     >  SRR4302543.4368303/1‑94 (MQ=60)
                     CTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAT                                                                     >  SRR4302543.3803412/1‑94 (MQ=60)
                      TATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATT                                                                    <  SRR4302543.6710726/94‑1 (MQ=60)
                      TATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATT                                                                    <  SRR4302543.110922/94‑1 (MQ=60)
                      TATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATT                                                                    >  SRR4302543.2015110/1‑94 (MQ=60)
                       ATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTT                                                                   >  SRR4302543.5161434/1‑94 (MQ=60)
                       ATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTT                                                                   >  SRR4302543.3761841/1‑94 (MQ=60)
                        TTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTT                                                                  >  SRR4302543.3465610/1‑94 (MQ=60)
                         TAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTT                                                                 <  SRR4302543.5794150/94‑1 (MQ=60)
                         TAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTT                                                                 >  SRR4302543.2175904/1‑94 (MQ=60)
                          AAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTC                                                                >  SRR4302543.5113534/1‑94 (MQ=60)
                           AATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCACAGTTCTGCTGAAATTTTTCT                                                               >  SRR4302543.396707/1‑94 (MQ=60)
                           AATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                               >  SRR4302543.2660557/1‑94 (MQ=60)
                           AATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                               >  SRR4302543.2413434/1‑94 (MQ=60)
                           AAGTGCTGTAGGCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCT                                                               <  SRR4302543.1747367/94‑1 (MQ=60)
                            ATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTG                                                              >  SRR4302543.1157032/1‑94 (MQ=60)
                             TTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                             <  SRR4302543.3215644/94‑1 (MQ=60)
                             TTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGT                                                             <  SRR4302543.1222472/94‑1 (MQ=60)
                              TGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTA                                                            <  SRR4302543.6851271/94‑1 (MQ=60)
                              TGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTA                                                            <  SRR4302543.2834130/94‑1 (MQ=60)
                               GCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAT                                                           >  SRR4302543.7521151/1‑94 (MQ=60)
                               GCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTAT                                                           <  SRR4302543.896212/94‑1 (MQ=60)
                                CTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATT                                                          >  SRR4302543.1143750/1‑94 (MQ=60)
                                CTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATT                                                          >  SRR4302543.7368323/1‑94 (MQ=60)
                                CTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATT                                                          >  SRR4302543.3903772/1‑94 (MQ=60)
                                CTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATT                                                          >  SRR4302543.6158177/1‑94 (MQ=60)
                                 TGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTA                                                         <  SRR4302543.5374429/94‑1 (MQ=60)
                                  GTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCATGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAA                                                        <  SRR4302543.4056947/94‑1 (MQ=60)
                                  GTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAA                                                        <  SRR4302543.901158/94‑1 (MQ=60)
                                  GTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAA                                                        >  SRR4302543.5076554/1‑94 (MQ=60)
                                   TAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAG                                                       <  SRR4302543.2870957/94‑1 (MQ=60)
                                   TAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAG                                                       <  SRR4302543.3513779/94‑1 (MQ=60)
                                   TAGTAGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAG                                                       <  SRR4302543.868573/94‑1 (MQ=60)
                                    AGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGG                                                      <  SRR4302543.6979402/94‑1 (MQ=60)
                                    AGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGG                                                      <  SRR4302543.7995785/94‑1 (MQ=60)
                                     GTCGTAAATAATTCTTTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGC                                                     >  SRR4302543.492123/1‑94 (MQ=60)
                                     GTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGC                                                     <  SRR4302543.4975449/94‑1 (MQ=60)
                                     GTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGC                                                     >  SRR4302543.3705994/1‑94 (MQ=60)
                                      TCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCG                                                    >  SRR4302543.724212/1‑94 (MQ=60)
                                        GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                  <  SRR4302543.1948191/94‑1 (MQ=60)
                                        GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                  >  SRR4302543.4097356/1‑94 (MQ=60)
                                        GTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAG                                                  >  SRR4302543.401597/1‑94 (MQ=60)
                                         TAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGA                                                 >  SRR4302543.149152/1‑94 (MQ=60)
                                          AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                <  SRR4302543.5466480/94‑1 (MQ=60)
                                          AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                >  SRR4302543.7852449/1‑94 (MQ=60)
                                          AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                >  SRR4302543.4796915/1‑94 (MQ=60)
                                          AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                <  SRR4302543.4762706/94‑1 (MQ=60)
                                          AAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAG                                                >  SRR4302543.2450267/1‑94 (MQ=60)
                                            ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                              >  SRR4302543.7643370/1‑94 (MQ=60)
                                            ATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTT                                              >  SRR4302543.1151514/1‑94 (MQ=60)
                                             TAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGATA                                             >  SRR4302543.1139642/1‑94 (MQ=60)
                                              AATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAT                                            <  SRR4302543.7473238/94‑1 (MQ=60)
                                              AATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTAT                                            <  SRR4302543.7511591/94‑1 (MQ=60)
                                               ATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATG                                           <  SRR4302543.205790/94‑1 (MQ=60)
                                               ATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATG                                           <  SRR4302543.6760569/94‑1 (MQ=60)
                                               ATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATG                                           >  SRR4302543.7701667/1‑94 (MQ=60)
                                               ATTCAGTGAGTATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAAGTTTGCTGTATTAAGGCGAGAGTTATG                                           <  SRR4302543.205789/94‑1 (MQ=60)
                                                TTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGT                                          <  SRR4302543.7442554/94‑1 (MQ=60)
                                                 TCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTG                                         <  SRR4302543.3316008/94‑1 (MQ=60)
                                                 TCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTG                                         <  SRR4302543.811047/94‑1 (MQ=60)
                                                  CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                        >  SRR4302543.7426646/1‑94 (MQ=60)
                                                  CATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGG                                        >  SRR4302543.962024/1‑94 (MQ=60)
                                                   ATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGA                                       <  SRR4302543.7252778/94‑1 (MQ=60)
                                                     TGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACG                                     <  SRR4302543.1484416/94‑1 (MQ=60)
                                                      GAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGA                                    <  SRR4302543.3861740/94‑1 (MQ=60)
                                                        GCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACG                                  <  SRR4302543.1507127/94‑1 (MQ=60)
                                                          ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                >  SRR4302543.1253513/1‑94 (MQ=60)
                                                          ATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAG                                <  SRR4302543.543535/94‑1 (MQ=60)
                                                           TTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGC                               <  SRR4302543.2725709/94‑1 (MQ=60)
                                                           TTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGC                               >  SRR4302543.2815187/1‑94 (MQ=60)
                                                           TTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGC                               <  SRR4302543.2160502/94‑1 (MQ=60)
                                                           TTCGTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGC                               >  SRR4302543.162495/1‑94 (MQ=60)
                                                              GTTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGG                            >  SRR4302543.2889790/1‑94 (MQ=60)
                                                               TTTCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGT                           <  SRR4302543.7777135/94‑1 (MQ=60)
                                                                 TCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGT                         >  SRR4302543.752841/1‑94 (MQ=60)
                                                                 TCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGT                         >  SRR4302543.1782190/1‑94 (MQ=60)
                                                                 TCACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGT                         >  SRR4302543.3100167/1‑94 (MQ=60)
                                                                  CACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTA                        <  SRR4302543.4686475/94‑1 (MQ=60)
                                                                   ACGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAG                       >  SRR4302543.6467692/1‑94 (MQ=60)
                                                                    CGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGG                      <  SRR4302543.470760/94‑1 (MQ=60)
                                                                    CGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGG                      >  SRR4302543.5125590/1‑94 (MQ=60)
                                                                    CGCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGG                      <  SRR4302543.2960056/94‑1 (MQ=60)
                                                                     GCCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGA                     <  SRR4302543.7354893/94‑1 (MQ=60)
                                                                      CCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGAT                    <  SRR4302543.1809897/94‑1 (MQ=60)
                                                                      CCAACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGAT                    <  SRR4302543.1925712/94‑1 (MQ=60)
                                                                        AACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAG                  >  SRR4302543.4286127/1‑94 (MQ=60)
                                                                         ACCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGG                 <  SRR4302543.3930733/94‑1 (MQ=60)
                                                                          CCTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGC                >  SRR4302543.1068759/1‑94 (MQ=60)
                                                                           CTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCT               >  SRR4302543.1488897/1‑94 (MQ=60)
                                                                           CTGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCT               >  SRR4302543.2793593/1‑94 (MQ=60)
                                                                            TGCCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTG              >  SRR4302543.5347042/1‑94 (MQ=60)
                                                                              CCAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTT            <  SRR4302543.4489553/94‑1 (MQ=60)
                                                                              CCAGTCAGGCAAGGCAAGCGCAAAGTTCGGCTGACATGTTTCTGTATTAAGGCGAGAGTTATGTGGTCGCCGAGCTGGTGTAGGATAGGCTGTT            <  SRR4302543.4489552/94‑1 (MQ=60)
                                                                               CAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTG           <  SRR4302543.6270603/94‑1 (MQ=60)
                                                                               CAGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTG           >  SRR4302543.1870265/1‑94 (MQ=60)
                                                                                AGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT          >  SRR4302543.78110/1‑94 (MQ=60)
                                                                                AGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGT          >  SRR4302543.6007023/1‑94 (MQ=60)
                                                                                AGTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGGTATGTGGACGACGAGCTGGTGTAGGATATGCTGTTGT          >  SRR4302543.6973803/1‑94 (MQ=60)
                                                                                 GTCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTT         >  SRR4302543.3334144/1‑94 (MQ=60)
                                                                                  TCAGGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTG        <  SRR4302543.4936230/94‑1 (MQ=60)
                                                                                     GGCAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTA     >  SRR4302543.1634662/1‑94 (MQ=60)
                                                                                       CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT   <  SRR4302543.4689682/94‑1 (MQ=60)
                                                                                       CAAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACT   <  SRR4302543.5711323/94‑1 (MQ=60)
                                                                                        AAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTG  >  SRR4302543.2020690/1‑94 (MQ=60)
                                                                                        AAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTG  <  SRR4302543.5403095/94‑1 (MQ=60)
                                                                                        AAGGCAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTG  >  SRR4302543.2162622/1‑94 (MQ=60)
                                                                                           |                                                                                          
ATCACGAACAAGATGCAAAAACTATTAAATTGCTGTAGTCGTAAATAATTCATTGAGCATTCGTTTCACGCCAACCTGCCAGTCAGGCAAGACAAGCGCAAAGTTCTGCTGAAATTTTTCTGTATTAAGGCGAGAGTTATGTGGACGACGAGCTGGTGTAGGATAGGCTGTTGTTGGTACTG  >  NC_000913/2109075‑2109256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑