Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,170,345 C→T G184E (GGA→GAA)  gatD ← galactitol‑1‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,170,3450CT100.0% 836.6 / NA 231G184E (GGA→GAA) gatDgalactitol‑1‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (113/118);  total (113/118)

Only 100 of 242 total aligned reads displayed.

TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434
                                                                                          |                                                                                         
tCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTtcc                                                                                         <  2:710779/93‑1 (MQ=255)
 cGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCa                                                                                        <  2:2302414/93‑1 (MQ=255)
  gCTACTGTTAAATGTTTACATCGCACCGAAAGATTTTGCCAGTGCCAGGTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAg                                                                                       >  2:3804748/1‑93 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGCACTACTGTCGATCGCCGTCACACTCTTTGCTTACAgc                                                                                      >  1:3530122/1‑93 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      >  2:3208327/1‑93 (MQ=255)
   cTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgc                                                                                      >  2:2084427/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  1:3421540/1‑93 (MQ=255)
    tACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcg                                                                                     >  1:2652309/1‑93 (MQ=255)
     aCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcgc                                                                                    >  1:5387076/1‑93 (MQ=255)
     aCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAgcgc                                                                                    >  1:22123/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  2:2408745/1‑93 (MQ=255)
       tGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGa                                                                                  >  2:599964/1‑93 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 <  2:1433710/93‑1 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 >  2:2924740/1‑93 (MQ=255)
        gTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGAc                                                                                 <  2:288687/93‑1 (MQ=255)
         ttAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACa                                                                                <  2:6956314/93‑1 (MQ=255)
          tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                               <  1:1941697/93‑1 (MQ=255)
          tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                               <  1:2755151/93‑1 (MQ=255)
          tAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAg                                                                               <  1:685091/93‑1 (MQ=255)
           aaaTGATTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgc                                                                              >  1:4110277/1‑93 (MQ=255)
              tGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAgcgcc                                                                           >  1:6882539/1‑92 (MQ=255)
              tGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCa                                                                           >  1:476309/1‑93 (MQ=255)
               gTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCAc                                                                          <  2:2802119/93‑1 (MQ=255)
                 ttGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAGTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTg                                                                        >  1:1217461/1‑93 (MQ=255)
                  tGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGa                                                                       <  1:6036872/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      <  1:4492976/93‑1 (MQ=255)
                   gCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGaa                                                                      >  1:1141146/1‑93 (MQ=255)
                    cATCGCACCGAAAGATTTTGCCAGGTCCAGTTTTTCTGAACTAATGGCGATCGCCTTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAt                                                                     <  2:1831209/93‑1 (MQ=255)
                     aTCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACCCTCTTTGCTTCCAGCGCGACAGCGCACTGAATg                                                                    <  2:7857497/93‑1 (MQ=255)
                      tCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATgg                                                                   >  1:3291702/1‑93 (MQ=255)
                       cGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGc                                                                  >  1:4460486/1‑93 (MQ=255)
                       cGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGc                                                                  >  2:3734208/1‑93 (MQ=255)
                        gCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACGGCGCCCTGAATGGcc                                                                 >  1:1367640/1‑93 (MQ=255)
                         cACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCca                                                                >  1:1337131/1‑93 (MQ=255)
                          aCCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcag                                                               >  2:3259445/1‑93 (MQ=255)
                           ccGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagc                                                              <  2:4095446/93‑1 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             <  1:2828088/93‑1 (MQ=255)
                            cGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagca                                                             <  1:1878410/93‑1 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            >  1:7553619/1‑93 (MQ=255)
                             gAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCcagcag                                                            <  1:5126861/93‑1 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          >  2:7115654/1‑93 (MQ=255)
                               aaGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGc                                                          >  1:7757690/1‑93 (MQ=255)
                                aGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGcc                                                         <  1:3984309/93‑1 (MQ=255)
                                 gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                        >  2:5681557/1‑93 (MQ=255)
                                 gATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCa                                                        >  2:3089057/1‑93 (MQ=255)
                                  aTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCaa                                                       >  1:5213806/1‑93 (MQ=255)
                                  aTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCaa                                                       <  1:4657368/93‑1 (MQ=255)
                                     ttGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATgg                                                    <  1:7396980/93‑1 (MQ=255)
                                       gccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGtt                                                  >  1:6305417/1‑93 (MQ=255)
                                        gcagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  1:6709607/92‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  1:3935918/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  2:5779986/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 <  1:1486432/93‑1 (MQ=255)
                                        ccagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTc                                                 >  2:6450872/1‑93 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                >  1:3809004/1‑93 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                <  2:264757/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                <  2:6418725/93‑1 (MQ=255)
                                         cagtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTcc                                                <  2:4861293/93‑1 (MQ=255)
                                          agtgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCg                                               >  2:4237734/1‑93 (MQ=255)
                                            tgccagtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGc                                             <  2:2275068/93‑1 (MQ=255)
                                                agtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCa                                         >  2:1400093/1‑93 (MQ=255)
                                                agtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCa                                         <  1:1016428/93‑1 (MQ=255)
                                                 gtTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaa                                        >  1:3684890/1‑93 (MQ=255)
                                                   ttttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaata                                      <  1:7175969/93‑1 (MQ=255)
                                                    tttCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataa                                     >  1:6591579/1‑93 (MQ=255)
                                                       cTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataa                                  <  1:7783091/93‑1 (MQ=255)
                                                        tGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataat                                 >  1:2355333/1‑93 (MQ=255)
                                                        tGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCaataataat                                 <  1:5707601/93‑1 (MQ=255)
                                                         gAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAataataata                                >  2:111695/1‑93 (MQ=255)
                                                          aaCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAtaataataa                               <  2:5006800/93‑1 (MQ=255)
                                                          aaCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAtaataataa                               >  1:2332535/1‑93 (MQ=255)
                                                           aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                              <  2:7431094/93‑1 (MQ=255)
                                                           aCTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAc                              <  2:1748106/93‑1 (MQ=255)
                                                            cTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACg                             <  1:6706003/93‑1 (MQ=255)
                                                             tAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGt                            >  2:297890/1‑93 (MQ=255)
                                                              aaTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           <  1:5999201/93‑1 (MQ=255)
                                                              aaTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           <  1:3024902/93‑1 (MQ=255)
                                                              aaTGTCAATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtt                           >  1:3359412/1‑93 (MQ=255)
                                                               aTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          <  1:4222968/93‑1 (MQ=255)
                                                               aTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttt                          <  2:6893561/93‑1 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         >  1:5950209/1‑93 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         <  2:3506836/93‑1 (MQ=255)
                                                                tGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGtttt                         <  2:1214496/93‑1 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        <  2:2529140/93‑1 (MQ=255)
                                                                 gTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGttttt                        <  2:2160634/93‑1 (MQ=255)
                                                                   cGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAAGTTTTTAt                      <  2:3582308/93‑1 (MQ=255)
                                                                    gATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAtt                     >  2:849983/1‑93 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 <  2:3381725/93‑1 (MQ=255)
                                                                        gCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCACGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTca                 >  2:7567723/1‑93 (MQ=255)
                                                                         ccGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTcac                >  2:1373890/1‑93 (MQ=255)
                                                                           gTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACaa              >  2:5969570/1‑93 (MQ=255)
                                                                            tCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAc             <  2:4429755/93‑1 (MQ=255)
                                                                              acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt           <  2:883711/93‑1 (MQ=255)
                                                                              acacTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCt           >  1:5531363/1‑93 (MQ=255)
                                                                                 ctctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgc        >  1:1600132/1‑93 (MQ=255)
                                                                                   ctTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTgcgc      >  1:1660677/1‑93 (MQ=255)
                                                                                    tttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCt     >  1:3245847/1‑93 (MQ=255)
                                                                                     ttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTa    >  1:6435611/1‑93 (MQ=255)
                                                                                     ttGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTa    >  1:5029566/1‑93 (MQ=255)
                                                                                       gCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTaaa  >  2:5633835/1‑93 (MQ=255)
                                                                                          |                                                                                         
TCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAA  >  NC_000913/2170255‑2170434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA  >  NC_000913/2170252‑2170437
                                                                                             |                                                                                            
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                              >  SRR4302543.2315083/1‑94 (MQ=60)
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTT                                                                                              >  SRR4302543.627831/1‑94 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                          <  SRR4302543.811999/94‑1 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                          <  SRR4302543.2562820/94‑1 (MQ=60)
    CGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAG                                                                                          >  SRR4302543.6866633/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGCACTACTGTCGATCGCCGTCACACTCTTTGCTTACAGC                                                                                         >  SRR4302543.3561565/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                         >  SRR4302543.6760982/1‑94 (MQ=60)
     GCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGC                                                                                         >  SRR4302543.3561566/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                        >  SRR4302543.2676359/1‑94 (MQ=60)
      CTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCG                                                                                        >  SRR4302543.3452156/1‑94 (MQ=60)
       TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                       >  SRR4302543.22328/1‑94 (MQ=60)
       TACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGC                                                                                       >  SRR4302543.5436013/1‑94 (MQ=60)
        ACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCG                                                                                      >  SRR4302543.3562864/1‑94 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     <  SRR4302543.6064760/94‑1 (MQ=60)
         CTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGA                                                                                     >  SRR4302543.7126864/1‑94 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                   <  SRR4302543.5961598/94‑1 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                   >  SRR4302543.274966/1‑94 (MQ=60)
           GTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACA                                                                                   <  SRR4302543.6920329/94‑1 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                 >  SRR4302543.5510725/1‑94 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                 <  SRR4302543.2780218/94‑1 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                 <  SRR4302543.691402/94‑1 (MQ=60)
             TAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                 <  SRR4302543.1959135/94‑1 (MQ=60)
             TAAATGATTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGC                                                                                 >  SRR4302543.4147269/1‑94 (MQ=60)
              AAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCG                                                                                >  SRR4302543.7511115/1‑94 (MQ=60)
                ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCC                                                                              >  SRR4302543.6947294/1‑94 (MQ=60)
                ATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCA                                                                              >  SRR4302543.480712/1‑94 (MQ=60)
                   TTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAGTGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTG                                                                           >  SRR4302543.1228405/1‑94 (MQ=60)
                   TTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTG                                                                           >  SRR4302543.1228406/1‑94 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                         <  SRR4302543.6093838/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                         <  SRR4302543.2852993/94‑1 (MQ=60)
                     TGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                         <  SRR4302543.4706444/94‑1 (MQ=60)
                     CGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAA                                                                         >  SRR4302543.1151540/1‑94 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                        <  SRR4302543.3295563/94‑1 (MQ=60)
                      GCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAAT                                                                        <  SRR4302543.4533807/94‑1 (MQ=60)
                       CATCGCACCGAAAGCTTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCTCACTGAATG                                                                       >  SRR4302543.2373127/1‑94 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                       >  SRR4302543.2373126/1‑94 (MQ=60)
                       CATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATG                                                                       >  SRR4302543.2802643/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                      >  SRR4302543.1630942/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                      >  SRR4302543.4520589/1‑94 (MQ=60)
                        ATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGG                                                                      >  SRR4302543.3321409/1‑94 (MQ=60)
                         TCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGC                                                                     >  SRR4302543.4500902/1‑94 (MQ=60)
                         TCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGC                                                                     >  SRR4302543.1246120/1‑94 (MQ=60)
                          CGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACGGCGCCCTGAATGGCC                                                                    >  SRR4302543.1379905/1‑94 (MQ=60)
                          CGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCC                                                                    >  SRR4302543.6231142/1‑94 (MQ=60)
                           GCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCA                                                                   >  SRR4302543.1349056/1‑94 (MQ=60)
                            CACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACCGCGCACTGAATGGCCAG                                                                  >  SRR4302543.4565439/1‑94 (MQ=60)
                              CCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCA                                                                <  SRR4302543.2329987/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                               >  SRR4302543.7625553/1‑94 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                               <  SRR4302543.2853959/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                               <  SRR4302543.1895287/94‑1 (MQ=60)
                               CGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAG                                                               <  SRR4302543.569563/94‑1 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGG                                                              <  SRR4302543.5173205/94‑1 (MQ=60)
                                GAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGC                                                              >  SRR4302543.5136615/1‑94 (MQ=60)
                                 AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                             >  SRR4302543.7830911/1‑94 (MQ=60)
                                 AAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGC                                                             >  SRR4302543.7830910/1‑94 (MQ=60)
                                   AGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCA                                                           <  SRR4302543.4019663/94‑1 (MQ=60)
                                    GATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAA                                                          >  SRR4302543.5260779/1‑94 (MQ=60)
                                     ATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                         >  SRR4302543.4747523/1‑94 (MQ=60)
                                     ATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                         <  SRR4302543.4699784/94‑1 (MQ=60)
                                     ATTGTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAAT                                                         <  SRR4302543.6018062/94‑1 (MQ=60)
                                       TTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGG                                                       <  SRR4302543.1519575/94‑1 (MQ=60)
                                       TTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGG                                                       <  SRR4302543.4232057/94‑1 (MQ=60)
                                        TTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGT                                                      <  SRR4302543.7468011/94‑1 (MQ=60)
                                         TGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTT                                                     >  SRR4302543.6364729/1‑94 (MQ=60)
                                           GCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   <  SRR4302543.6772973/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   <  SRR4302543.1499806/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   >  SRR4302543.3842585/1‑94 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   <  SRR4302543.3970694/94‑1 (MQ=60)
                                           CCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCC                                                   <  SRR4302543.45157/94‑1 (MQ=60)
                                            CAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCG                                                  <  SRR4302543.3097526/94‑1 (MQ=60)
                                                GCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGACTGGCCAGCAGGCCAATGGTTCCGGCTC                                              >  SRR4302543.1043090/1‑94 (MQ=60)
                                                 CCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACC                                             >  SRR4302543.7295062/1‑94 (MQ=60)
                                                  CAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCA                                            >  SRR4302543.181732/1‑94 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                           >  SRR4302543.1435164/1‑94 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                           <  SRR4302543.1025418/94‑1 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                           <  SRR4302543.6429537/94‑1 (MQ=60)
                                                   AGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAA                                           >  SRR4302543.3717269/1‑94 (MQ=60)
                                                    GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                          <  SRR4302543.145611/94‑1 (MQ=60)
                                                    GTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAAT                                          >  SRR4302543.481506/1‑94 (MQ=60)
                                                      TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                        <  SRR4302543.7821802/94‑1 (MQ=60)
                                                      TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                        >  SRR4302543.6653680/1‑94 (MQ=60)
                                                      TTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAA                                        <  SRR4302543.7245633/94‑1 (MQ=60)
                                                       TTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAAT                                       <  SRR4302543.6873873/94‑1 (MQ=60)
                                                          CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                    <  SRR4302543.7856472/94‑1 (MQ=60)
                                                          CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                    <  SRR4302543.2891202/94‑1 (MQ=60)
                                                          CTGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAAT                                    >  SRR4302543.2376693/1‑94 (MQ=60)
                                                           TGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATA                                   <  SRR4302543.5759669/94‑1 (MQ=60)
                                                           TGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATA                                   >  SRR4302543.7623593/1‑94 (MQ=60)
                                                           TGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATA                                   >  SRR4302543.1939178/1‑94 (MQ=60)
                                                           TGAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATA                                   >  SRR4302543.6113602/1‑94 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                  <  SRR4302543.3056077/94‑1 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                  <  SRR4302543.2230593/94‑1 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                  <  SRR4302543.3757696/94‑1 (MQ=60)
                                                            GAACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAA                                  >  SRR4302543.2353706/1‑94 (MQ=60)
                                                             AACTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAAC                                 >  SRR4302543.1354846/1‑94 (MQ=60)
                                                               CTAATGTGGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                               <  SRR4302543.6769313/94‑1 (MQ=60)
                                                               CTAATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGT                               <  SRR4302543.6769312/94‑1 (MQ=60)
                                                                TAATGTCAATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTT                              >  SRR4302543.3389630/1‑94 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                             <  SRR4302543.6054284/94‑1 (MQ=60)
                                                                 AATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                             <  SRR4302543.3052238/94‑1 (MQ=60)
                                                                 AATGTCGATCGCCGACACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTT                             >  SRR4302543.3475284/1‑94 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            <  SRR4302543.4261262/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            <  SRR4302543.4373371/94‑1 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            >  SRR4302543.3354306/1‑94 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            >  SRR4302543.6004889/1‑94 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            >  SRR4302543.5495195/1‑94 (MQ=60)
                                                                  ATGTCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTT                            >  SRR4302543.6004663/1‑94 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                         <  SRR4302543.3973509/94‑1 (MQ=60)
                                                                     TCGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTAT                         <  SRR4302543.3866741/94‑1 (MQ=60)
                                                                      CGATCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATT                        <  SRR4302543.5273434/94‑1 (MQ=60)
                                                                         TCGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTC                     <  SRR4302543.3921817/94‑1 (MQ=60)
                                                                          CGCCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCA                    >  SRR4302543.1735812/1‑94 (MQ=60)
                                                                            CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                  <  SRR4302543.3434913/94‑1 (MQ=60)
                                                                            CCGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                  >  SRR4302543.4224320/1‑94 (MQ=60)
                                                                            CCGTCACACTCTTTGCCTCCAGCGCGACAGCGCACTGAATGGCCAGCACGTCAATCGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACA                  >  SRR4302543.4090773/1‑94 (MQ=60)
                                                                             CGTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATAGCCAGAAGGCCAATGGTTCCGGCACCAATACTACTAAAGTTATTGTTCTCACAA                 >  SRR4302543.2688113/1‑94 (MQ=60)
                                                                              GTCACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAAC                >  SRR4302543.6631524/1‑94 (MQ=60)
                                                                                CACACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCT              >  SRR4302543.5581620/1‑94 (MQ=60)
                                                                                 ACACTCTTTGCGTCCAGCGCGACAGCTCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTT             <  SRR4302543.3437958/94‑1 (MQ=60)
                                                                                  CACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTG            >  SRR4302543.6620696/1‑94 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC           <  SRR4302543.5259457/94‑1 (MQ=60)
                                                                                   ACTCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGC           >  SRR4302543.1614410/1‑94 (MQ=60)
                                                                                    CTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCG          <  SRR4302543.7179183/94‑1 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         >  SRR4302543.1191223/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         >  SRR4302543.5236344/1‑94 (MQ=60)
                                                                                     TCTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGC         >  SRR4302543.1675554/1‑94 (MQ=60)
                                                                                      CTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT        >  SRR4302543.3275198/1‑94 (MQ=60)
                                                                                      CTTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCT        >  SRR4302543.2220577/1‑94 (MQ=60)
                                                                                       TTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTA       >  SRR4302543.5075330/1‑94 (MQ=60)
                                                                                       TTTGCTTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTA       >  SRR4302543.6496250/1‑94 (MQ=60)
                                                                                            TTCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA  <  SRR4302543.3641476/94‑1 (MQ=60)
                                                                                             |                                                                                            
ATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGA  >  NC_000913/2170252‑2170437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑