Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,177,832	T→C	V331A (GTG→GCG)	yegT →	putative nucleoside permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,177,832	0	T	C	99.6%	825.3 / ‑5.1	263	V331A (GTG→GCG)	yegT	putative nucleoside permease protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (140/122); total (140/123)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

Only 100 of 269 total aligned reads displayed.

GTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGTGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGA  >  NC_000913/2177743‑2177921

gTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCa                                                                                        >  2:3206791/1‑93 (MQ=255)

  tACTGTTCCTCGGTTTTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTTCTTCTATTGCGATAAAAAAGCCCCCGCGCata                                                                                      <  2:6179388/93‑1 (MQ=255)

  tACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCata                                                                                      <  1:4187063/93‑1 (MQ=255)

  tACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCata                                                                                      <  2:1091994/93‑1 (MQ=255)

   tcTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCatat                                                                                     <  1:720196/92‑1 (MQ=255)

   aCTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCatat                                                                                     >  2:4394669/1‑93 (MQ=255)

    cTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATg                                                                                    >  1:5618130/1‑93 (MQ=255)

     tGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGc                                                                                   >  2:639525/1‑93 (MQ=255)

     cgtgccTCGGTCTTTTGCTTCACGGCGTAAGTTACGATTTTTTCTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGc                                                                                   <  1:5397753/89‑1 (MQ=255)

      gTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCg                                                                                  >  1:2351570/1‑93 (MQ=255)

         ccTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTAc                                                                               <  1:462730/93‑1 (MQ=255)

         ccTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTAc                                                                               <  2:2761553/93‑1 (MQ=255)

          cTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTAcc                                                                              <  2:1858200/93‑1 (MQ=255)

           tCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTAccc                                                                             >  2:1959484/1‑92 (MQ=255)

            cGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGc                                                                            >  1:4431140/1‑93 (MQ=255)

            cGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGc                                                                            <  1:4901900/93‑1 (MQ=255)

            cGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGc                                                                            >  2:5572417/1‑93 (MQ=255)

             ggTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCt                                                                           >  2:7310100/1‑93 (MQ=255)

               tATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTgc                                                                         >  2:3508255/1‑93 (MQ=255)

                aTTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTgcg                                                                        >  2:131898/1‑93 (MQ=255)

                  tttGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCa                                                                      >  1:1214362/1‑93 (MQ=255)

                  tttGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCa                                                                      <  2:7752534/93‑1 (MQ=255)

                    tGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAgg                                                                    <  1:1538592/93‑1 (MQ=255)

                    tGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAgg                                                                    <  2:3006042/93‑1 (MQ=255)

                     gCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAggg                                                                   >  1:2941947/1‑93 (MQ=255)

                       ttCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGc                                                                 >  1:7324442/1‑93 (MQ=255)

                       ttCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGc                                                                 <  1:6411488/93‑1 (MQ=255)

                       ttCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGc                                                                 >  1:5532280/1‑93 (MQ=255)

                       ttCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCACCGCGCATATGCGTACCGCTGCGCAGGGGc                                                                 >  2:6521202/1‑93 (MQ=255)

                       ttCACGGCGTAAGATACGATTTTTACTACGTTACCGGTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCCGCGCAGGGGc                                                                 >  2:6116377/1‑93 (MQ=255)

                        tCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGTGCATATGCGTACCGCTGCGCAGGGGCt                                                                <  2:7215290/93‑1 (MQ=255)

                          aCGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGa                                                              <  2:4515924/93‑1 (MQ=255)

                          aCGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGa                                                              <  1:5196951/93‑1 (MQ=255)

                           cGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGAt                                                             <  1:6144776/93‑1 (MQ=255)

                             gCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCa                                                           <  1:1342909/93‑1 (MQ=255)

                               gTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACg                                                         >  2:679407/1‑93 (MQ=255)

                               gTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACg                                                         >  1:7847013/1‑93 (MQ=255)

                               gTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACg                                                         >  1:3975515/1‑93 (MQ=255)

                                tAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGc                                                        >  1:2163573/1‑93 (MQ=255)

                                 aaGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGct                                                       <  2:6936625/93‑1 (MQ=255)

                                  aGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGctc                                                      <  1:3028723/93‑1 (MQ=255)

                                   gTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGctct                                                     >  1:2005198/1‑93 (MQ=255)

                                     tACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTctgc                                                   <  1:2247869/93‑1 (MQ=255)

                                         aTTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGcc                                               <  1:3179917/93‑1 (MQ=255)

                                         aTTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGcc                                               <  2:2428848/93‑1 (MQ=255)

                                           ttttACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAg                                             <  1:4318361/93‑1 (MQ=255)

                                           ttttACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAg                                             >  1:2520835/1‑93 (MQ=255)

                                           ttttACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAg                                             <  2:6710560/93‑1 (MQ=255)

                                            tttACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAgg                                            <  1:3290289/93‑1 (MQ=255)

                                                ctacGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCtt                                        >  2:6228539/1‑93 (MQ=255)

                                                ctacGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCtt                                        >  1:836012/1‑93 (MQ=255)

                                                 tacGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTc                                       >  1:4072739/1‑93 (MQ=255)

                                                 tacGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTc                                       >  2:1700189/1‑93 (MQ=255)

                                                 tacGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTc                                       >  2:1619910/1‑93 (MQ=255)

                                                  acGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCg                                      >  1:6504205/1‑93 (MQ=255)

                                                  acGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCg                                      <  2:2658151/93‑1 (MQ=255)

                                                    gTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGc                                    >  1:7687149/1‑93 (MQ=255)

                                                     ttACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCa                                   >  1:2403519/1‑93 (MQ=255)

                                                      tACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAg                                  <  1:1955017/93‑1 (MQ=255)

                                                       aCCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGt                                 >  2:2262691/1‑93 (MQ=255)

                                                        ccGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGtt                                <  1:6735912/93‑1 (MQ=255)

                                                         cGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGttt                               >  1:2718818/1‑93 (MQ=255)

                                                         cGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAAttt                               <  2:7745797/93‑1 (MQ=255)

                                                          gCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTg                              >  2:871477/1‑93 (MQ=255)

                                                          gCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTg                              >  1:3390708/1‑93 (MQ=255)

                                                          gCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTg                              >  1:2226157/1‑93 (MQ=255)

                                                           cTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGc                             >  2:4700619/1‑93 (MQ=255)

                                                           cTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGc                             >  2:5676024/1‑93 (MQ=255)

                                                           cTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGc                             >  2:7912535/1‑93 (MQ=255)

                                                             tACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTc                           <  1:343877/93‑1 (MQ=255)

                                                              aCATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCg                          >  1:2809308/1‑93 (MQ=255)

                                                               cATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCgg                         >  1:6246120/1‑93 (MQ=255)

                                                                aTCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGc                        <  1:3816609/93‑1 (MQ=255)

                                                                aTCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGc                        >  1:7702000/1‑93 (MQ=255)

                                                                aTCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGc                        <  2:4272097/93‑1 (MQ=255)

                                                                aTCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGc                        >  2:5180715/1‑93 (MQ=255)

                                                                aTCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGc                        >  2:5224967/1‑93 (MQ=255)

                                                                 tCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTAGGCAGTTTGCTCGGCt                       >  2:2616862/1‑93 (MQ=255)

                                                                   tATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTAt                     <  2:7105996/93‑1 (MQ=255)

                                                                   tATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTAt                     <  2:5107541/93‑1 (MQ=255)

                                                                    aTGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATc                    <  2:2790937/93‑1 (MQ=255)

                                                                      gTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGt                  >  1:5029012/1‑93 (MQ=255)

                                                                      gTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGt                  <  1:6381346/93‑1 (MQ=255)

                                                                       tCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTc                 >  1:1098779/1‑93 (MQ=255)

                                                                        cGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCt                <  2:5889377/93‑1 (MQ=255)

                                                                         gATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCtt               <  2:4005891/93‑1 (MQ=255)

                                                                         gATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCtt               <  2:3783525/93‑1 (MQ=255)

                                                                         gATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCtt               <  2:3323798/93‑1 (MQ=255)

                                                                          aTAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTg              <  1:3682184/93‑1 (MQ=255)

                                                                           tAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTgg             >  2:1406130/1‑93 (MQ=255)

                                                                           aaaaaaaGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTgg             <  2:3659852/92‑1 (MQ=255)

                                                                            aaaaaaGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGc            >  1:1653593/1‑93 (MQ=255)

                                                                             aaaaaGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCg           >  2:6601305/1‑93 (MQ=255)

                                                                             aaaaaGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCg           >  1:7489130/1‑93 (MQ=255)

                                                                                aaGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGgtg        <  1:1435628/93‑1 (MQ=255)

                                                                                   cccccGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGtga     <  1:4702001/93‑1 (MQ=255)

                                                                                    ccccGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGtgat    <  2:1435802/93‑1 (MQ=255)

                                                                                     cccGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGtgatg   >  2:6472400/1‑93 (MQ=255)

                                                                                     cccGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGtgatg   <  1:6041622/93‑1 (MQ=255)

                                                                                      ccGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGtgatga  >  1:7143970/1‑93 (MQ=255)

GTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGTGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGA  >  NC_000913/2177743‑2177921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGTGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGA  >  NC_000913/2177742‑2177925

CGTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCA                                                                                            >  SRR4302543.5647058/1‑94 (MQ=60)

CGTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCA                                                                                            >  SRR4302543.7845517/1‑94 (MQ=60)

 GTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCAT                                                                                           >  SRR4302543.1972675/1‑94 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         <  SRR4302543.2410591/94‑1 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         <  SRR4302543.6770093/94‑1 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         <  SRR4302543.168782/94‑1 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         >  SRR4302543.1394124/1‑94 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         <  SRR4302543.6467485/94‑1 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         <  SRR4302543.1641298/94‑1 (MQ=60)

   TACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATAT                                                                                         <  SRR4302543.4225022/94‑1 (MQ=60)

    TCTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATG                                                                                        <  SRR4302543.726699/94‑1 (MQ=60)

    ACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATG                                                                                        >  SRR4302543.5669325/1‑94 (MQ=60)

      cgtgCCTCGGTCTTTTGCTTCACGGCGTAAGTTACGATTTTTTCTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCG                                                                                      <  SRR4302543.5446841/90‑1 (MQ=60)

      TGTTGCTCGGTATTTTGCTTCACGGCGTAATTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCG                                                                                      <  SRR4302543.4918611/94‑1 (MQ=60)

      TGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCG                                                                                      >  SRR4302543.2372894/1‑94 (MQ=60)

      TGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCG                                                                                      <  SRR4302543.293673/94‑1 (MQ=60)

       GTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGT                                                                                     <  SRR4302543.4807875/94‑1 (MQ=60)

         TCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTAC                                                                                   <  SRR4302543.4471166/94‑1 (MQ=60)

          CCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACC                                                                                  >  SRR4302543.2181886/1‑94 (MQ=60)

          CCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACC                                                                                  <  SRR4302543.467034/94‑1 (MQ=60)

            TCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGC                                                                                >  SRR4302543.4471280/1‑94 (MQ=60)

             CGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCT                                                                               >  SRR4302543.4652895/1‑94 (MQ=60)

             CGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCT                                                                               <  SRR4302543.4946585/94‑1 (MQ=60)

             CGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCT                                                                               >  SRR4302543.2247870/1‑94 (MQ=60)

              GGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCAACCGCGCATATGCGTACCGCTG                                                                              >  SRR4302543.4430671/1‑94 (MQ=60)

               GTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGC                                                                             >  SRR4302543.4346719/1‑94 (MQ=60)

               GTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGC                                                                             >  SRR4302543.406933/1‑94 (MQ=60)

                TATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCG                                                                            >  SRR4302543.3645803/1‑94 (MQ=60)

                 ATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGC                                                                           >  SRR4302543.6450027/1‑94 (MQ=60)

                 ATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGC                                                                           >  SRR4302543.6326728/1‑94 (MQ=60)

                 ATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGC                                                                           >  SRR4302543.4330212/1‑94 (MQ=60)

                 ATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGC                                                                           >  SRR4302543.7581048/1‑94 (MQ=60)

                  TTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCA                                                                          >  SRR4302543.1225271/1‑94 (MQ=60)

                    TTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGG                                                                        <  SRR4302543.116834/94‑1 (MQ=60)

                     TGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGG                                                                       <  SRR4302543.5311522/94‑1 (MQ=60)

                     TGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGG                                                                       <  SRR4302543.1552372/94‑1 (MQ=60)

                     TGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGG                                                                       >  SRR4302543.2968659/1‑94 (MQ=60)

                       CTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGC                                                                     >  SRR4302543.5582547/1‑94 (MQ=60)

                       CTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGC                                                                     >  SRR4302543.5582549/1‑94 (MQ=60)

                       CTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGC                                                                     >  SRR4302543.7394972/1‑94 (MQ=60)

                        TTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCT                                                                    <  SRR4302543.6471866/94‑1 (MQ=60)

                         TCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTG                                                                   <  SRR4302543.3599993/94‑1 (MQ=60)

                           ACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGAT                                                                 <  SRR4302543.5243808/94‑1 (MQ=60)

                           ACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGAT                                                                 <  SRR4302543.7774509/94‑1 (MQ=60)

                            CGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATC                                                                <  SRR4302543.4725949/94‑1 (MQ=60)

                            CGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATC                                                                <  SRR4302543.6202970/94‑1 (MQ=60)

                             GGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCA                                                               >  SRR4302543.3001866/1‑94 (MQ=60)

                             GGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCA                                                               <  SRR4302543.3424129/94‑1 (MQ=60)

                              GCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCAC                                                              <  SRR4302543.1354902/94‑1 (MQ=60)

                              GCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCAC                                                              <  SRR4302543.1253568/94‑1 (MQ=60)

                               CGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACG                                                             >  SRR4302543.5568205/1‑94 (MQ=60)

                               CGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACG                                                             >  SRR4302543.894865/1‑94 (MQ=60)

                               CGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACG                                                             >  SRR4302543.7920967/1‑94 (MQ=60)

                               CGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACG                                                             >  SRR4302543.4010757/1‑94 (MQ=60)

                                GTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCTCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGC                                                            >  SRR4302543.339015/1‑94 (MQ=60)

                                GTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGC                                                            >  SRR4302543.2182962/1‑94 (MQ=60)

                                GTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGC                                                            >  SRR4302543.3197213/1‑94 (MQ=60)

                                GTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGC                                                            >  SRR4302543.4015580/1‑94 (MQ=60)

                                   AGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCT                                                         >  SRR4302543.2023217/1‑94 (MQ=60)

                                   AGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCT                                                         >  SRR4302543.2023216/1‑94 (MQ=60)

                                   AGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCT                                                         <  SRR4302543.7309252/94‑1 (MQ=60)

                                   AGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCT                                                         <  SRR4302543.3056081/94‑1 (MQ=60)

                                    GTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTG                                                        <  SRR4302543.3720792/94‑1 (MQ=60)

                                    ATTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTG                                                        <  SRR4302543.6270995/94‑1 (MQ=60)

                                      TACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCT                                                      <  SRR4302543.2268279/94‑1 (MQ=60)

                                      TACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCT                                                      <  SRR4302543.1519315/94‑1 (MQ=60)

                                          ATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCA                                                  <  SRR4302543.3208586/94‑1 (MQ=60)

                                           TTTTTACTACGTTACCGCTTCCATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAG                                                 <  SRR4302543.4988169/94‑1 (MQ=60)

                                           TTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAG                                                 <  SRR4302543.7235530/94‑1 (MQ=60)

                                           TTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAG                                                 >  SRR4302543.2543748/1‑94 (MQ=60)

                                            TTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGG                                                <  SRR4302543.1336985/94‑1 (MQ=60)

                                            TTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGG                                                <  SRR4302543.81539/94‑1 (MQ=60)

                                            TTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGG                                                <  SRR4302543.4357578/94‑1 (MQ=60)

                                             TTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGG                                               <  SRR4302543.3319987/94‑1 (MQ=60)

                                               TACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCT                                             <  SRR4302543.2435288/94‑1 (MQ=60)

                                               TACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCAGCCAGGGGT                                             >  SRR4302543.7908798/1‑94 (MQ=60)

                                                ACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCTGCGCATATGCGGACGGATGCGCAGGGGATGATCACGCTCTGCTGCCAGGGCTT                                            >  SRR4302543.2812560/1‑94 (MQ=60)

                                                ACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTT                                            >  SRR4302543.843393/1‑94 (MQ=60)

                                                ACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTT                                            >  SRR4302543.748417/1‑94 (MQ=60)

                                                 CTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTC                                           >  SRR4302543.4109225/1‑94 (MQ=60)

                                                 CTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTC                                           >  SRR4302543.4109226/1‑94 (MQ=60)

                                                 CTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTC                                           >  SRR4302543.5326061/1‑94 (MQ=60)

                                                 CTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTC                                           >  SRR4302543.5101729/1‑94 (MQ=60)

                                                  TACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCG                                          >  SRR4302543.2914492/1‑94 (MQ=60)

                                                  TACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCG                                          >  SRR4302543.6565473/1‑94 (MQ=60)

                                                   ACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGG                                         <  SRR4302543.3310132/94‑1 (MQ=60)

                                                    CGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGC                                        >  SRR4302543.6629568/1‑94 (MQ=60)

                                                    CGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGC                                        >  SRR4302543.7759945/1‑94 (MQ=60)

                                                     GTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCA                                       <  SRR4302543.6889012/94‑1 (MQ=60)

                                                     GTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCA                                       >  SRR4302543.2425258/1‑94 (MQ=60)

                                                       TACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGT                                     >  SRR4302543.344468/1‑94 (MQ=60)

                                                       TACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGT                                     <  SRR4302543.1972551/94‑1 (MQ=60)

                                                       TACCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGT                                     >  SRR4302543.2326425/1‑94 (MQ=60)

                                                         CCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTT                                   >  SRR4302543.2743527/1‑94 (MQ=60)

                                                         CCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTT                                   <  SRR4302543.6799541/94‑1 (MQ=60)

                                                         CCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTT                                   <  SRR4302543.6799538/94‑1 (MQ=60)

                                                         CCGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTT                                   <  SRR4302543.2409129/94‑1 (MQ=60)

                                                          CGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTG                                  >  SRR4302543.2246252/1‑94 (MQ=60)

                                                          CGCTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTG                                  >  SRR4302543.3421112/1‑94 (MQ=60)

                                                            CTTACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCT                                >  SRR4302543.137246/1‑94 (MQ=60)

                                                              TACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCG                              >  SRR4302543.2835023/1‑94 (MQ=60)

                                                              TACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCG                              <  SRR4302543.347057/94‑1 (MQ=60)

                                                               ACATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGG                             >  SRR4302543.6305046/1‑94 (MQ=60)

                                                                CATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGC                            >  SRR4302543.7774890/1‑94 (MQ=60)

                                                                CATCTATGTCGATAAAAAAGACCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGCCTTCCGCAGTTTGCTCGGC                            >  SRR4302543.2831768/1‑94 (MQ=60)

                                                                 ATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCT                           >  SRR4302543.3894221/1‑94 (MQ=60)

                                                                 ATCTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCT                           <  SRR4302543.3850255/94‑1 (MQ=60)

                                                                   CTATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTAT                         >  SRR4302543.1644445/1‑94 (MQ=60)

                                                                    TATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATC                        >  SRR4302543.2552966/1‑94 (MQ=60)

                                                                     ATGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCCCGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCG                       <  SRR4302543.7483001/94‑1 (MQ=60)

                                                                      TGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGT                      >  SRR4302543.3299187/1‑94 (MQ=60)

                                                                      TGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGT                      >  SRR4302543.3145634/1‑94 (MQ=60)

                                                                      TGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGT                      >  SRR4302543.6052096/1‑94 (MQ=60)

                                                                      TGTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGT                      >  SRR4302543.5074774/1‑94 (MQ=60)

                                                                       GTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTC                     >  SRR4302543.1108675/1‑94 (MQ=60)

                                                                       GTCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTC                     <  SRR4302543.6441394/94‑1 (MQ=60)

                                                                        TCGATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCT                    >  SRR4302543.815357/1‑94 (MQ=60)

                                                                          GATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTG                  >  SRR4302543.2308425/1‑94 (MQ=60)

                                                                           ATAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGG                 <  SRR4302543.3714540/94‑1 (MQ=60)

                                                                            TAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGC                >  SRR4302543.1668389/1‑94 (MQ=60)

                                                                            TAAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGC                <  SRR4302543.1944755/94‑1 (MQ=60)

                                                                             AAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCG               <  SRR4302543.6168808/94‑1 (MQ=60)

                                                                             AAAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCG               >  SRR4302543.7560788/1‑94 (MQ=60)

                                                                              AAAAAGCCCCCGCGCATATGCGTACCGCTTCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGG              >  SRR4302543.2228474/1‑94 (MQ=60)

                                                                              AAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGG              >  SRR4302543.2228733/1‑94 (MQ=60)

                                                                              AAAAAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGG              >  SRR4302543.7717770/1‑94 (MQ=60)

                                                                                 AAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGGTCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGT           <  SRR4302543.6541953/94‑1 (MQ=60)

                                                                                 AAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGT           <  SRR4302543.6373370/94‑1 (MQ=60)

                                                                                 AAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGT           <  SRR4302543.1448606/94‑1 (MQ=60)

                                                                                 AAGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGG           >  SRR4302543.721863/1‑94 (MQ=60)

                                                                                  AGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTG          <  SRR4302543.4589465/94‑1 (MQ=60)

                                                                                  AGCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTG          <  SRR4302543.4997421/94‑1 (MQ=60)

                                                                                   GCCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGA         >  SRR4302543.3878855/1‑94 (MQ=60)

                                                                                    CCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGAT        <  SRR4302543.4744953/94‑1 (MQ=60)

                                                                                    CCCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGAT        >  SRR4302543.265944/1‑94 (MQ=60)

                                                                                     CCGCGCGCATATGCGTACCGCTGCGCAGAGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATG       <  SRR4302543.5627580/94‑1 (MQ=60)

                                                                                     CCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATG       >  SRR4302543.5679824/1‑94 (MQ=60)

                                                                                     CCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATG       >  SRR4302543.281509/1‑94 (MQ=60)

                                                                                     CCCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGGGAGATG       >  SRR4302543.2584717/1‑94 (MQ=60)

                                                                                      CCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGA      >  SRR4302543.7213390/1‑94 (MQ=60)

                                                                                      CCCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGA      <  SRR4302543.6098802/94‑1 (MQ=60)

                                                                                       CCGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGAT     <  SRR4302543.618653/94‑1 (MQ=60)

                                                                                        CGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATG    <  SRR4302543.2596380/94‑1 (MQ=60)

                                                                                        CGCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGCCGGTGCGATGGTG    >  SRR4302543.2672049/1‑94 (MQ=60)

                                                                                         GCGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGG   <  SRR4302543.2846813/94‑1 (MQ=60)

                                                                                          CGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGA  >  SRR4302543.3051237/1‑94 (MQ=60)

                                                                                          CGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGA  >  SRR4302543.7616421/1‑94 (MQ=60)

                                                                                          CGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGA  >  SRR4302543.3360080/1‑94 (MQ=60)

CGTTACTGTTCCTCGGTATTTTGCTTCACGGCGTAAGTTACGATTTTTACTACGTTACCGCTTACATCTATGTCGATAAAAAAGCCCCCGTGCATATGCGTACCGCTGCGCAGGGGCTGATCACGCTCTGCTGCCAGGGCTTCGGCAGTTTGCTCGGCTATCGTCTTGGCGGTGTGATGATGGA  >  NC_000913/2177742‑2177925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`