Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,204,258	T→C	W182R (TGG→CGG)	yehM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,204,258	0	T	C	100.0%	658.1 / NA	213	W182R (TGG→CGG)	yehM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (95/118); total (95/118)

Only 100 of 251 total aligned reads displayed.

CCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGC  >  NC_000913/2204168‑2204347

cccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgg                                                                                         >  2:2530826/1‑93 (MQ=255)

cccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgg                                                                                         >  1:4392125/1‑93 (MQ=255)

 ccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgga                                                                                        <  2:7443258/93‑1 (MQ=255)

 ccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgga                                                                                        >  1:4802352/1‑93 (MQ=255)

 ccTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcgga                                                                                        >  2:4464429/1‑93 (MQ=255)

  ctctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggat                                                                                       >  1:6845602/1‑93 (MQ=255)

   tctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggatt                                                                                      <  1:5908409/93‑1 (MQ=255)

   tctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggatt                                                                                      >  2:728866/1‑93 (MQ=255)

    ctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattg                                                                                     <  1:3479542/93‑1 (MQ=255)

    ctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattg                                                                                     <  2:6250129/93‑1 (MQ=255)

    ctGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattg                                                                                     <  2:4093764/93‑1 (MQ=255)

     tGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattgg                                                                                    <  1:7648516/93‑1 (MQ=255)

     tGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattgg                                                                                    >  2:6746521/1‑93 (MQ=255)

     tGCGCTGGCGCACTATAATGCCCTACTGCGGGGCGACTCCACCGGCGATGCGCTTAACCGTTTGCGCGAAGCCTTGACGGCCTGCcggatttt                                                                                    >  2:6992805/1‑91 (MQ=255)

       cgcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCcggattggat                                                                                  <  2:5864541/93‑1 (MQ=255)

        gcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATg                                                                                 <  1:3973160/93‑1 (MQ=255)

        gcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATg                                                                                 >  1:6059943/1‑93 (MQ=255)

        gcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATg                                                                                 <  2:3936482/93‑1 (MQ=255)

        gcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATg                                                                                 <  2:3172091/93‑1 (MQ=255)

        gcTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATg                                                                                 <  2:1726762/93‑1 (MQ=255)

         cTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATgg                                                                                <  1:2875293/93‑1 (MQ=255)

          tGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATggg                                                                               >  2:34999/1‑93 (MQ=255)

           ggCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGc                                                                              <  1:834487/93‑1 (MQ=255)

           ggCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGc                                                                              <  1:3032227/93‑1 (MQ=255)

            gcgcACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCg                                                                             <  1:2473744/93‑1 (MQ=255)

             cgcACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGa                                                                            <  1:1027370/93‑1 (MQ=255)

             cgcACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGa                                                                            <  1:3056658/93‑1 (MQ=255)

                aCTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgc                                                                         <  2:6539782/93‑1 (MQ=255)

                aCTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgc                                                                         <  1:6451455/93‑1 (MQ=255)

                 cTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgca                                                                        <  2:496034/93‑1 (MQ=255)

                 cTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgca                                                                        <  1:1644793/93‑1 (MQ=255)

                 cTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGAGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgga                                                                        >  2:2024264/1‑91 (MQ=255)

                    ttttGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATgcagca                                                                     >  2:2886803/1‑93 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               >  2:4928860/1‑93 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               <  2:4484710/93‑1 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               <  2:7484867/93‑1 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               <  2:1231599/93‑1 (MQ=255)

                          ccAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataa                                                               >  1:7057762/1‑93 (MQ=255)

                           cAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAaataat                                                              <  1:7255250/93‑1 (MQ=255)

                            aaCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATg                                                             >  2:5687992/1‑93 (MQ=255)

                             aCTGCGGGGGGAGGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATgg                                                            <  2:1592970/93‑1 (MQ=255)

                             aCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATgg                                                            >  2:1972912/1‑93 (MQ=255)

                             aCTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATgg                                                            <  2:1421916/93‑1 (MQ=255)

                              cTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATAGc                                                           >  1:3602184/1‑93 (MQ=255)

                               tGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCg                                                          >  2:4120799/1‑93 (MQ=255)

                                gCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGa                                                         <  2:2790487/93‑1 (MQ=255)

                                  ggggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACg                                                       <  1:5438199/93‑1 (MQ=255)

                                   gggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACgt                                                      >  1:4962459/1‑93 (MQ=255)

                                    ggCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGGCgtg                                                     >  2:2800399/1‑93 (MQ=255)

                                       gACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTa                                                  >  2:7214445/1‑93 (MQ=255)

                                       gACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTa                                                  >  2:4340445/1‑93 (MQ=255)

                                        aCGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAg                                                 >  2:1127792/1‑93 (MQ=255)

                                        aCGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAg                                                 <  1:4649919/93‑1 (MQ=255)

                                          gCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGtt                                               <  1:1582308/93‑1 (MQ=255)

                                          gCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGtt                                               <  2:3552404/93‑1 (MQ=255)

                                          gCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGtt                                               <  1:3144100/93‑1 (MQ=255)

                                           ccTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTg                                              <  2:831650/93‑1 (MQ=255)

                                           ccTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTg                                              >  2:6068546/1‑93 (MQ=255)

                                            cTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGt                                             >  1:4162889/1‑93 (MQ=255)

                                            cTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGt                                             <  1:7543744/93‑1 (MQ=255)

                                              ccGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCt                                           <  1:22435/93‑1 (MQ=255)

                                               cGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTg                                          >  1:1920345/1‑93 (MQ=255)

                                               cGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTg                                          >  1:6736104/1‑93 (MQ=255)

                                                ggCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGc                                         <  2:4658752/93‑1 (MQ=255)

                                                 gCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTTTTAGTTGTCTGCg                                        <  2:2554136/93‑1 (MQ=255)

                                                 gCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCg                                        <  1:2042644/93‑1 (MQ=255)

                                                   gATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCggg                                      >  2:4663892/1‑92 (MQ=255)

                                                   gATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGt                                      <  1:338442/93‑1 (MQ=255)

                                                   gATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGAGACGTGTTAGTTGCCTGctgg                                      >  2:3711060/1‑90 (MQ=255)

                                                    aTGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtg                                     >  1:5239764/1‑93 (MQ=255)

                                                     tGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtgg                                    <  2:7814195/93‑1 (MQ=255)

                                                       cgcTCAATCGTCAGCGCGAAGCCTTTATGCCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggct                                  >  2:5016900/1‑93 (MQ=255)

                                                          tCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggctggc                               >  1:6386772/1‑93 (MQ=255)

                                                          tCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGtggctggc                               <  2:5777638/93‑1 (MQ=255)

                                                            aaTCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCAc                             <  1:1533508/93‑1 (MQ=255)

                                                              tCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGc                           >  2:231959/1‑93 (MQ=255)

                                                                 tCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTcc                        >  1:4050968/1‑93 (MQ=255)

                                                                  cAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCg                       >  2:3155646/1‑93 (MQ=255)

                                                                   aGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGGGGGTGGCACGCTCCgg                      >  1:2423675/1‑93 (MQ=255)

                                                                     cgcgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCa                    <  2:4816279/93‑1 (MQ=255)

                                                                      gcgAAGCTTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCAc                   <  1:7215435/93‑1 (MQ=255)

                                                                      gcgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCAc                   >  1:6187643/1‑93 (MQ=255)

                                                                      gcgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCAc                   <  2:5390029/93‑1 (MQ=255)

                                                                       cgAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACt                  <  2:3415569/93‑1 (MQ=255)

                                                                         aaGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTgg                <  1:7902264/93‑1 (MQ=255)

                                                                          aGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGc               >  1:1798942/1‑93 (MQ=255)

                                                                            ccTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCaa             <  1:6310262/93‑1 (MQ=255)

                                                                             cTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCaaa            <  1:5554050/93‑1 (MQ=255)

                                                                              tttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAg           <  2:1676093/93‑1 (MQ=255)

                                                                              tttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAg           <  2:3897737/93‑1 (MQ=255)

                                                                               ttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAgc          >  2:2849942/1‑92 (MQ=255)

                                                                               ttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGa          <  2:4357177/93‑1 (MQ=255)

                                                                               ttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGa          <  2:7739210/93‑1 (MQ=255)

                                                                               ttATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGa          >  1:931382/1‑93 (MQ=255)

                                                                                tATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAt         <  1:1184795/93‑1 (MQ=255)

                                                                                   ggCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGAtgtg      <  1:3832704/93‑1 (MQ=255)

                                                                                   ggCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGAtgtg      <  2:5353926/93‑1 (MQ=255)

                                                                                    gCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTgg     <  1:2698948/93‑1 (MQ=255)

                                                                                    gCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGATGTgg     <  1:1713906/93‑1 (MQ=255)

                                                                                      ccgccgGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGgcg   >  1:3244400/1‑93 (MQ=255)

CCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGC  >  NC_000913/2204168‑2204347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  >  NC_000913/2204166‑2204351

TACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCG                                                                                              >  SRR4302543.6426610/1‑94 (MQ=60)

 ACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGG                                                                                             >  SRR4302543.4431942/1‑94 (MQ=60)

  CCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGA                                                                                            >  SRR4302543.4846236/1‑94 (MQ=60)

   CCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGAT                                                                                           >  SRR4302543.6572172/1‑94 (MQ=60)

   CCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGAT                                                                                           >  SRR4302543.6910197/1‑94 (MQ=60)

   CCGCTGCGCGTGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCTCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGAT                                                                                           <  SRR4302543.5145568/94‑1 (MQ=60)

    CTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATT                                                                                          <  SRR4302543.6262000/94‑1 (MQ=60)

    CTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATT                                                                                          <  SRR4302543.3091587/94‑1 (MQ=60)

    CTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATT                                                                                          >  SRR4302543.4102894/1‑94 (MQ=60)

     TCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTG                                                                                         <  SRR4302543.5962343/94‑1 (MQ=60)

      CTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGG                                                                                        <  SRR4302543.4984517/94‑1 (MQ=60)

      CTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGG                                                                                        <  SRR4302543.3510706/94‑1 (MQ=60)

       TGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGA                                                                                       <  SRR4302543.7721036/94‑1 (MQ=60)

        GCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGAT                                                                                      <  SRR4302543.1635385/94‑1 (MQ=60)

        GCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGAT                                                                                      <  SRR4302543.2838346/94‑1 (MQ=60)

        GCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGAT                                                                                      <  SRR4302543.5298133/94‑1 (MQ=60)

        GCGCGGGCGCACTATTTGGCCCGACCGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCAAAGCCTTTATGGCCCGCCGGATTGGAG                                                                                      <  SRR4302543.7791633/94‑1 (MQ=60)

         CGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATG                                                                                     >  SRR4302543.6117314/1‑94 (MQ=60)

          GCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGG                                                                                    <  SRR4302543.4008379/94‑1 (MQ=60)

          GCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGG                                                                                    <  SRR4302543.5412383/94‑1 (MQ=60)

          GCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGG                                                                                    >  SRR4302543.6395251/1‑94 (MQ=60)

          GCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGG                                                                                    >  SRR4302543.1660090/1‑94 (MQ=60)

           CTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGG                                                                                   >  SRR4302543.761366/1‑94 (MQ=60)

           CTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGG                                                                                   <  SRR4302543.2327042/94‑1 (MQ=60)

           CTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGG                                                                                   >  SRR4302543.6662056/1‑94 (MQ=60)

           CTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGG                                                                                   <  SRR4302543.2901466/94‑1 (MQ=60)

            TGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGC                                                                                  <  SRR4302543.3228907/94‑1 (MQ=60)

             GGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCG                                                                                 <  SRR4302543.3059606/94‑1 (MQ=60)

             GGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCG                                                                                 <  SRR4302543.841853/94‑1 (MQ=60)

              GCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGA                                                                                <  SRR4302543.2496095/94‑1 (MQ=60)

               CGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGAT                                                                               <  SRR4302543.3084175/94‑1 (MQ=60)

               CGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGAT                                                                               <  SRR4302543.6089152/94‑1 (MQ=60)

               CGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGAT                                                                               <  SRR4302543.1036473/94‑1 (MQ=60)

                GCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATG                                                                              <  SRR4302543.4982983/94‑1 (MQ=60)

                  CCTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCA                                                                            <  SRR4302543.4781923/94‑1 (MQ=60)

                  ACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCA                                                                            <  SRR4302543.6512251/94‑1 (MQ=60)

                   CTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAG                                                                           >  SRR4302543.6850742/1‑94 (MQ=60)

                   CTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAG                                                                           <  SRR4302543.1659482/94‑1 (MQ=60)

                    TATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGC                                                                          >  SRR4302543.1591954/1‑94 (MQ=60)

                     ATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCA                                                                         >  SRR4302543.1812721/1‑94 (MQ=60)

                         TGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAAT                                                                     <  SRR4302543.7301163/94‑1 (MQ=60)

                          GCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATA                                                                    <  SRR4302543.5903648/94‑1 (MQ=60)

                           CCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAA                                                                   >  SRR4302543.7126255/1‑94 (MQ=60)

                            CCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAAT                                                                  <  SRR4302543.4409136/94‑1 (MQ=60)

                            CCAACTGCGGGGCGACGCATGCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAAT                                                                  <  SRR4302543.3047025/94‑1 (MQ=60)

                             CAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATG                                                                 >  SRR4302543.6986780/1‑94 (MQ=60)

                             CAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATG                                                                 >  SRR4302543.400391/1‑94 (MQ=60)

                             CAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATG                                                                 <  SRR4302543.929607/94‑1 (MQ=60)

                             CAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATG                                                                 <  SRR4302543.7325390/94‑1 (MQ=60)

                              AACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGG                                                                >  SRR4302543.1802600/1‑94 (MQ=60)

                               ACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGC                                                               <  SRR4302543.6173761/94‑1 (MQ=60)

                               ACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATAGC                                                               >  SRR4302543.3633972/1‑94 (MQ=60)

                                  GCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGAC                                                            <  SRR4302543.807273/94‑1 (MQ=60)

                                   CGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACG                                                           <  SRR4302543.6964341/94‑1 (MQ=60)

                                   CGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACG                                                           <  SRR4302543.636841/94‑1 (MQ=60)

                                    GGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGT                                                          <  SRR4302543.5487578/94‑1 (MQ=60)

                                    GGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGT                                                          >  SRR4302543.5007634/1‑94 (MQ=60)

                                     GGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTG                                                         <  SRR4302543.3595241/94‑1 (MQ=60)

                                       GCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTT                                                       <  SRR4302543.5124822/94‑1 (MQ=60)

                                       GCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTT                                                       <  SRR4302543.3122326/94‑1 (MQ=60)

                                        CGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTA                                                      >  SRR4302543.737774/1‑94 (MQ=60)

                                         GACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAG                                                     >  SRR4302543.161752/1‑94 (MQ=60)

                                          ACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGT                                                    <  SRR4302543.4037745/94‑1 (MQ=60)

                                          ACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGT                                                    <  SRR4302543.3061563/94‑1 (MQ=60)

                                          ACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGT                                                    <  SRR4302543.4692235/94‑1 (MQ=60)

                                           CGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTT                                                   <  SRR4302543.2132994/94‑1 (MQ=60)

                                            GCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTG                                                  <  SRR4302543.1596424/94‑1 (MQ=60)

                                            GCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTG                                                  <  SRR4302543.3172382/94‑1 (MQ=60)

                                             CCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGT                                                 >  SRR4302543.4200576/1‑94 (MQ=60)

                                              CTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTC                                                <  SRR4302543.7615627/94‑1 (MQ=60)

                                              CTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTAGATGGGCCATGCAGCCAAATAATGGCGACGTGTTAGTTGTC                                                >  SRR4302543.4132076/1‑94 (MQ=60)

                                               TCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCTCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCT                                               <  SRR4302543.7319697/94‑1 (MQ=60)

                                               TCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCT                                               <  SRR4302543.810750/94‑1 (MQ=60)

                                                CCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTG                                              >  SRR4302543.6799732/1‑94 (MQ=60)

                                                CCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTG                                              <  SRR4302543.22641/94‑1 (MQ=60)

                                                CCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTG                                              >  SRR4302543.1937653/1‑94 (MQ=60)

                                                 CGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGC                                             <  SRR4302543.1448706/94‑1 (MQ=60)

                                                   GCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGG                                           <  SRR4302543.2060960/94‑1 (MQ=60)

                                                    CGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGT                                          >  SRR4302543.4568614/1‑94 (MQ=60)

                                                     GATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTG                                         >  SRR4302543.5286986/1‑94 (MQ=60)

                                                     GATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTG                                         <  SRR4302543.341551/94‑1 (MQ=60)

                                                      ATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGG                                        <  SRR4302543.7231981/94‑1 (MQ=60)

                                                       gctGCTCAATCGTCAGCGCGAAGCCTTTATGTCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGC                                       <  SRR4302543.769790/91‑1 (MQ=60)

                                                           CTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGC                                   >  SRR4302543.6446888/1‑94 (MQ=60)

                                                            TCAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCA                                  >  SRR4302543.1001337/1‑94 (MQ=60)

                                                             CAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCAC                                 <  SRR4302543.5679647/94‑1 (MQ=60)

                                                             CAATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCAC                                 <  SRR4302543.2923664/94‑1 (MQ=60)

                                                              AATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACG                                >  SRR4302543.3450751/1‑94 (MQ=60)

                                                              AATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACG                                <  SRR4302543.1547249/94‑1 (MQ=60)

                                                               ATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGC                               >  SRR4302543.1895170/1‑94 (MQ=60)

                                                               ATCGTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGC                               >  SRR4302543.2720551/1‑94 (MQ=60)

                                                                  GTCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCC                            >  SRR4302543.4087213/1‑94 (MQ=60)

                                                                   GCAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCG                           <  SRR4302543.1207762/94‑1 (MQ=60)

                                                                    CAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGG                          >  SRR4302543.5488438/1‑94 (MQ=60)

                                                                    CAGCGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGGGGGTGGCACGCTCCGG                          >  SRR4302543.2445552/1‑94 (MQ=60)

                                                                       CGCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCAC                       >  SRR4302543.6246107/1‑94 (MQ=60)

                                                                        GCGAAGCTTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACT                      <  SRR4302543.7285374/94‑1 (MQ=60)

                                                                        GCGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACT                      <  SRR4302543.7818190/94‑1 (MQ=60)

                                                                         CGAAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTTGCTGGCACGCTCCGGCACTG                     <  SRR4302543.648268/94‑1 (MQ=60)

                                                                           AAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGC                   <  SRR4302543.3331351/94‑1 (MQ=60)

                                                                           AAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGC                   <  SRR4302543.7976540/94‑1 (MQ=60)

                                                                           AAGCCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGC                   >  SRR4302543.1815050/1‑94 (MQ=60)

                                                                              CCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAA                >  SRR4302543.1103964/1‑94 (MQ=60)

                                                                              CCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAA                >  SRR4302543.4559063/1‑94 (MQ=60)

                                                                              CCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAA                <  SRR4302543.3905731/94‑1 (MQ=60)

                                                                              CCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAA                <  SRR4302543.6369608/94‑1 (MQ=60)

                                                                              CCTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAA                <  SRR4302543.2984574/94‑1 (MQ=60)

                                                                               CTTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAG               <  SRR4302543.5604569/94‑1 (MQ=60)

                                                                                TTTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGA              >  SRR4302543.939525/1‑94 (MQ=60)

                                                                                 TTATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGAT             <  SRR4302543.2479435/94‑1 (MQ=60)

                                                                                  TATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATG            <  SRR4302543.1195492/94‑1 (MQ=60)

                                                                                  TATGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATG            <  SRR4302543.3795512/94‑1 (MQ=60)

                                                                                    TGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTG          >  SRR4302543.1981756/1‑94 (MQ=60)

                                                                                    TGGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGATGTG          <  SRR4302543.3383624/94‑1 (MQ=60)

                                                                                     GGCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGATGTGG         <  SRR4302543.3866514/94‑1 (MQ=60)

                                                                                      GCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGC        <  SRR4302543.2723421/94‑1 (MQ=60)

                                                                                      GCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGC        <  SRR4302543.2346768/94‑1 (MQ=60)

                                                                                      GCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGC        >  SRR4302543.1969683/1‑94 (MQ=60)

                                                                                      GCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGC        <  SRR4302543.6214467/94‑1 (MQ=60)

                                                                                      GCCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGATGTGGC        <  SRR4302543.1729227/94‑1 (MQ=60)

                                                                                       CCCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCG       >  SRR4302543.3273739/1‑94 (MQ=60)

                                                                                        CCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGC      <  SRR4302543.3561144/94‑1 (MQ=60)

                                                                                        CCGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCCCCGGCACTGGCAAAGATGTGGCGC      <  SRR4302543.250203/94‑1 (MQ=60)

                                                                                         CGCCGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCG     >  SRR4302543.547946/1‑94 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  >  SRR4302543.5454430/1‑94 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  <  SRR4302543.1440678/94‑1 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  <  SRR4302543.413947/94‑1 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  <  SRR4302543.4860106/94‑1 (MQ=60)

                                                                                            CGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  <  SRR4302543.7045896/94‑1 (MQ=60)

TACCCTCTGCGCTGGCGCACTATTTTGCCCAACTGCGGGGCGACGCCTCCGGCGATGCGCTCAATCGTCAGCGCGAAGCCTTTATGGCCCGCTGGATTGGATGGGCGATGCAGCAAAATAATGGCGACGTGTTAGTTGTCTGCGGTGGCTGGCACGCTCCGGCACTGGCAAAGATGTGGCGCGAAT  >  NC_000913/2204166‑2204351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`