Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,216,746 G→C S252S (TCC→TCG)  yehZ ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,216,7460GC100.0% 851.7 / NA 269S252S (TCC→TCG) yehZputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (140/129);  total (140/129)

Only 100 of 281 total aligned reads displayed.

GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC  >  NC_000913/2216656‑2216834
                                                                                          |                                                                                        
  tGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGCAGACTGGCTGTAGCCACTGTGCCATTTGCGGATCCTCCCTCCACACCGACt                                                                                      >  2:5979801/1‑93 (MQ=255)
  tGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGGAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACt                                                                                      >  1:3688668/1‑93 (MQ=255)
      aTTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACg                                                                                  <  2:1628852/93‑1 (MQ=255)
        tCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGca                                                                                <  1:986176/93‑1 (MQ=255)
        tCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGca                                                                                >  2:1564457/1‑93 (MQ=255)
         cAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcac                                                                               <  1:3044433/93‑1 (MQ=255)
         cAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcac                                                                               <  1:2852268/93‑1 (MQ=255)
          aGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcacc                                                                              <  1:3207294/93‑1 (MQ=255)
             tgctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACt                                                                           >  2:3399612/1‑93 (MQ=255)
               ttgcAATGTTTTTGCAGCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCAATTTGCGGATACGCCCTCAACACCGACTCACGCACCACTgg                                                                         <  1:5711823/92‑1 (MQ=255)
               ctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGcaccaccag                                                                         >  1:6958850/1‑90 (MQ=255)
               ctgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTgg                                                                         <  2:7089925/93‑1 (MQ=255)
                tgcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCAATTGCGGATACTCCCTCAACAACGACTCACCCACCACTgcg                                                                        >  2:3523464/1‑91 (MQ=255)
                 gcAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTg                                                                       >  1:1003076/1‑93 (MQ=255)
                  cAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGc                                                                      >  1:5947481/1‑93 (MQ=255)
                   aaTGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCACCCACCCCTGGTGCa                                                                     >  1:6106183/1‑93 (MQ=255)
                   aaTGTTTTTGCATCGAGGCTGGCGAAGAATGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCACGCACCACTGGTGCa                                                                     >  1:7215430/1‑93 (MQ=255)
                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                                   <  2:106762/93‑1 (MQ=255)
                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAgg                                                                   <  2:5613069/93‑1 (MQ=255)
                     tGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTACACACCGACTCACGCACCACTGGTGCAgg                                                                   >  1:2932853/1‑93 (MQ=255)
                         tttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                                               <  2:7341601/93‑1 (MQ=255)
                         tttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                                               <  2:5986498/93‑1 (MQ=255)
                         tttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGcgcg                                                               >  1:1306611/1‑93 (MQ=255)
                          ttGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGt                                                              <  1:1859381/93‑1 (MQ=255)
                           tGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTa                                                             <  1:4035856/93‑1 (MQ=255)
                             cATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGa                                                           <  2:1131418/93‑1 (MQ=255)
                             cATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGa                                                           <  2:4070053/93‑1 (MQ=255)
                              aTCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAt                                                          >  1:4060915/1‑93 (MQ=255)
                               tcaaGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                         <  1:922145/90‑1 (MQ=255)
                               tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                         >  1:5353770/1‑93 (MQ=255)
                               tCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATa                                                         >  2:5424568/1‑93 (MQ=255)
                                cGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAg                                                        <  2:6184227/93‑1 (MQ=255)
                                 gAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAgg                                                       <  1:5066950/93‑1 (MQ=255)
                                  aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt                                                      <  2:2306304/93‑1 (MQ=255)
                                  aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGt                                                      <  1:6613541/93‑1 (MQ=255)
                                  aGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGGAGATAGGt                                                      >  2:1859781/1‑93 (MQ=255)
                                   ggCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGtt                                                     <  1:280862/93‑1 (MQ=255)
                                    gCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTg                                                    <  2:1961706/93‑1 (MQ=255)
                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                  <  2:2766377/93‑1 (MQ=255)
                                      tGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGca                                                  <  2:861610/93‑1 (MQ=255)
                                        gCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGcaca                                                <  2:4959846/93‑1 (MQ=255)
                                         ggAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCacac                                               <  1:7345368/92‑1 (MQ=255)
                                          gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc                                              <  1:2408411/93‑1 (MQ=255)
                                          gAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACAcc                                              >  2:7852211/1‑93 (MQ=255)
                                           aaGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCt                                             >  2:7449397/1‑93 (MQ=255)
                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                            >  1:5597859/1‑93 (MQ=255)
                                            aGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCtt                                            <  1:170534/93‑1 (MQ=255)
                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                           >  1:1075430/1‑93 (MQ=255)
                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                           >  1:7867820/1‑93 (MQ=255)
                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                           <  1:7535246/93‑1 (MQ=255)
                                             gACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTg                                           >  1:3378766/1‑93 (MQ=255)
                                              aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                          <  1:2261597/93‑1 (MQ=255)
                                              aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                          <  2:4858219/93‑1 (MQ=255)
                                              aCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCAATGGTGCAGGCGCGTAGATAGGTTGCACACCTTGc                                          <  2:4637858/93‑1 (MQ=255)
                                               cTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCg                                         >  1:88808/1‑93 (MQ=255)
                                                tGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCgg                                        >  1:3913701/1‑93 (MQ=255)
                                                 ggCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGa                                       <  1:3813561/93‑1 (MQ=255)
                                                   cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                     <  1:2781004/93‑1 (MQ=255)
                                                   cTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATc                                     >  2:6815276/1‑93 (MQ=255)
                                                    tGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCg                                    <  1:4771684/93‑1 (MQ=255)
                                                     gTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGc                                   <  1:5816671/93‑1 (MQ=255)
                                                          cACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAg                              >  2:2816856/1‑93 (MQ=255)
                                                           aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg                             <  2:5139717/93‑1 (MQ=255)
                                                           aCTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAgg                             <  2:6957935/93‑1 (MQ=255)
                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                           <  1:3497686/93‑1 (MQ=255)
                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                           >  2:1806712/1‑93 (MQ=255)
                                                             tgtgCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                           <  2:648597/93‑1 (MQ=255)
                                                             tgtgCCATTTGCGGATACTACCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGtt                           <  2:7017802/93‑1 (MQ=255)
                                                                gCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc                        <  2:3736592/93‑1 (MQ=255)
                                                                gCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc                        >  2:4390054/1‑93 (MQ=255)
                                                                gCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGc                        >  2:781520/1‑93 (MQ=255)
                                                                 gctgttGCGGATACTCCCTCAACCCCGACTCCCACACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa                       <  1:796078/89‑1 (MQ=255)
                                                                 ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa                       >  2:420420/1‑93 (MQ=255)
                                                                 ccATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCa                       >  1:6702716/1‑93 (MQ=255)
                                                                  cATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAg                      >  2:6360560/1‑93 (MQ=255)
                                                                   aTTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGc                     >  1:4128659/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                    >  2:2906334/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                    >  1:7454805/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                    >  2:2433785/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                    >  2:5285595/1‑93 (MQ=255)
                                                                    tttGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcc                    >  2:2212970/1‑93 (MQ=255)
                                                                      tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcccc                  <  1:2077006/93‑1 (MQ=255)
                                                                      tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcccc                  <  1:687713/93‑1 (MQ=255)
                                                                      tGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGcccc                  <  1:6062692/93‑1 (MQ=255)
                                                                        cGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAg                <  1:844770/93‑1 (MQ=255)
                                                                        cGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAg                <  1:4903977/93‑1 (MQ=255)
                                                                         ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgc               <  1:136730/93‑1 (MQ=255)
                                                                         ggATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgc               <  1:4663181/93‑1 (MQ=255)
                                                                          gATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAgcg              >  2:1302233/1‑93 (MQ=255)
                                                                                cccTCAACCCCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTa        <  2:5052520/93‑1 (MQ=255)
                                                                                cccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTa        >  1:457542/1‑93 (MQ=255)
                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc       >  2:2148839/1‑93 (MQ=255)
                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc       <  1:6740301/93‑1 (MQ=255)
                                                                                 ccTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAc       <  1:153417/93‑1 (MQ=255)
                                                                                  cTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTAcc      <  1:4222089/93‑1 (MQ=255)
                                                                                   tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg     >  2:6926717/1‑93 (MQ=255)
                                                                                   tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg     >  2:2646504/1‑93 (MQ=255)
                                                                                   tCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCg     <  2:7898315/93‑1 (MQ=255)
                                                                                    cAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCgg    <  1:5896933/93‑1 (MQ=255)
                                                                                     aaCACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCggg   <  1:6227197/93‑1 (MQ=255)
                                                                                          |                                                                                        
GCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC  >  NC_000913/2216656‑2216834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  NC_000913/2216653‑2216839
                                                                                             |                                                                                             
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGGCTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                               <  SRR4302543.5782671/94‑1 (MQ=60)
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                               <  SRR4302543.4501579/94‑1 (MQ=60)
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACC                                                                                               >  SRR4302543.5306608/1‑94 (MQ=60)
 ATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCG                                                                                              >  SRR4302543.5597008/1‑94 (MQ=60)
    CTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACT                                                                                           <  SRR4302543.1080104/94‑1 (MQ=60)
    CTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACT                                                                                           >  SRR4302543.2930903/1‑94 (MQ=60)
    CTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACT                                                                                           >  SRR4302543.738546/1‑94 (MQ=60)
    CTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGGAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACT                                                                                           >  SRR4302543.3721086/1‑94 (MQ=60)
     TGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTC                                                                                          <  SRR4302543.5096496/94‑1 (MQ=60)
         ATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGC                                                                                      >  SRR4302543.6091655/1‑94 (MQ=60)
         ATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGC                                                                                      >  SRR4302543.5884736/1‑94 (MQ=60)
           TCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCAC                                                                                    <  SRR4302543.994876/94‑1 (MQ=60)
            CAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACC                                                                                   <  SRR4302543.2878296/94‑1 (MQ=60)
            CAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACC                                                                                   <  SRR4302543.3071884/94‑1 (MQ=60)
            CAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACC                                                                                   >  SRR4302543.851534/1‑94 (MQ=60)
             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA                                                                                  <  SRR4302543.3236237/94‑1 (MQ=60)
             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA                                                                                  >  SRR4302543.6176897/1‑94 (MQ=60)
             AGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCA                                                                                  >  SRR4302543.4258227/1‑94 (MQ=60)
              GTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCAC                                                                                 >  SRR4302543.886419/1‑94 (MQ=60)
              GTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCAC                                                                                 <  SRR4302543.4030737/94‑1 (MQ=60)
                 GCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACcag                                                                              >  SRR4302543.7024325/1‑91 (MQ=60)
                 GCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGG                                                                              <  SRR4302543.7529419/94‑1 (MQ=60)
                  TTGCAATGTTTTTGCAGCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCAATTTGCGGATACGCCCTCAACACCGACTCACGCACCACTGGT                                                                             <  SRR4302543.5763935/94‑1 (MQ=60)
                  CTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGT                                                                             >  SRR4302543.868875/1‑94 (MQ=60)
                   TGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTG                                                                            >  SRR4302543.1011942/1‑94 (MQ=60)
                   TGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTG                                                                            <  SRR4302543.6789369/94‑1 (MQ=60)
                   TGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTG                                                                            >  SRR4302543.7283612/1‑94 (MQ=60)
                   TGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACCCACCACTGGTG                                                                            >  SRR4302543.4895018/1‑94 (MQ=60)
                    GCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGC                                                                           >  SRR4302543.6001915/1‑94 (MQ=60)
                     CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCA                                                                          <  SRR4302543.5185453/94‑1 (MQ=60)
                     CAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCACCCACCCCTGGTGCA                                                                          >  SRR4302543.6164035/1‑94 (MQ=60)
                     CAATGTTTTTGCATCGAGGCTGGCGAAGAATGGCTGTAGCCACTGTGCCATTTGCGGATAATCCCTCAACACCGACTCACGCACCACTGGTGCA                                                                          >  SRR4302543.7285369/1‑94 (MQ=60)
                       ATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTACACACCGACTCACGCACCACTGGTGCAGG                                                                        >  SRR4302543.2959486/1‑94 (MQ=60)
                          TTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGC                                                                     >  SRR4302543.798690/1‑94 (MQ=60)
                           TTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGgtgacgggcg                                                                    >  SRR4302543.616811/1‑84 (MQ=60)
                           TTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCG                                                                    >  SRR4302543.1318216/1‑94 (MQ=60)
                           TTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCG                                                                    <  SRR4302543.1460085/94‑1 (MQ=60)
                             TTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTA                                                                  <  SRR4302543.1876091/94‑1 (MQ=60)
                              TGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAG                                                                 <  SRR4302543.4071959/94‑1 (MQ=60)
                              TGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAG                                                                 <  SRR4302543.391246/94‑1 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               <  SRR4302543.4081611/94‑1 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               >  SRR4302543.4097279/1‑94 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               >  SRR4302543.3044485/1‑94 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               <  SRR4302543.1994253/94‑1 (MQ=60)
                                CATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGAT                                                               <  SRR4302543.7300107/94‑1 (MQ=60)
                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              >  SRR4302543.3112168/1‑94 (MQ=60)
                                 ATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATA                                                              >  SRR4302543.5402222/1‑94 (MQ=60)
                                  TCAAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAG                                                             <  SRR4302543.930215/94‑1 (MQ=60)
                                   CGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGG                                                            >  SRR4302543.2035559/1‑94 (MQ=60)
                                    GAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGT                                                           >  SRR4302543.3588969/1‑94 (MQ=60)
                                    GAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGT                                                           <  SRR4302543.5112948/94‑1 (MQ=60)
                                    GAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGT                                                           >  SRR4302543.909322/1‑94 (MQ=60)
                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                          <  SRR4302543.4618125/94‑1 (MQ=60)
                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                          >  SRR4302543.1329569/1‑94 (MQ=60)
                                     AGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTT                                                          <  SRR4302543.6675891/94‑1 (MQ=60)
                                      GGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTG                                                         >  SRR4302543.4546033/1‑94 (MQ=60)
                                      GGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTG                                                         <  SRR4302543.283351/94‑1 (MQ=60)
                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       <  SRR4302543.5571520/94‑1 (MQ=60)
                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       >  SRR4302543.1490395/1‑94 (MQ=60)
                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       >  SRR4302543.3427271/1‑94 (MQ=60)
                                        CTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCA                                                       >  SRR4302543.3427480/1‑94 (MQ=60)
                                         TGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCAC                                                      >  SRR4302543.7972569/1‑94 (MQ=60)
                                            GGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACC                                                   <  SRR4302543.7416032/94‑1 (MQ=60)
                                            CGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACC                                                   >  SRR4302543.7563000/1‑94 (MQ=60)
                                             GAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCT                                                  <  SRR4302543.2430180/94‑1 (MQ=60)
                                             GAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCT                                                  >  SRR4302543.1588929/1‑94 (MQ=60)
                                             GAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCT                                                  >  SRR4302543.6165997/1‑94 (MQ=60)
                                              AAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTT                                                 >  SRR4302543.5648858/1‑94 (MQ=60)
                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                <  SRR4302543.171993/94‑1 (MQ=60)
                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                >  SRR4302543.3409092/1‑94 (MQ=60)
                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                >  SRR4302543.1085097/1‑94 (MQ=60)
                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                >  SRR4302543.7941903/1‑94 (MQ=60)
                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTG                                                >  SRR4302543.483187/1‑94 (MQ=60)
                                               AGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATACGTTGCCCACCTTC                                                >  SRR4302543.3409091/1‑94 (MQ=60)
                                                GACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGC                                               <  SRR4302543.7607093/94‑1 (MQ=60)
                                                GACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGC                                               <  SRR4302543.7607094/94‑1 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              <  SRR4302543.2282223/94‑1 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              >  SRR4302543.89634/1‑94 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              >  SRR4302543.3736769/1‑94 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              <  SRR4302543.2282224/94‑1 (MQ=60)
                                                 ACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCG                                              >  SRR4302543.4036516/1‑94 (MQ=60)
                                                  CTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGG                                             >  SRR4302543.3948250/1‑94 (MQ=60)
                                                   TGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGA                                            >  SRR4302543.6815708/1‑94 (MQ=60)
                                                    GGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                           <  SRR4302543.811004/94‑1 (MQ=60)
                                                    GGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                           <  SRR4302543.5406188/94‑1 (MQ=60)
                                                    GGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGAT                                           <  SRR4302543.3847179/94‑1 (MQ=60)
                                                     GCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATC                                          <  SRR4302543.2874719/94‑1 (MQ=60)
                                                      CTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCG                                         <  SRR4302543.2806349/94‑1 (MQ=60)
                                                      CTGTAGCCACTGTGCCATTTGAGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGTCGCGTAGATAGGTTGCACACCTTGCGGATCC                                         >  SRR4302543.2827565/1‑94 (MQ=60)
                                                       TGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGC                                        <  SRR4302543.3129839/94‑1 (MQ=60)
                                                       TGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGC                                        <  SRR4302543.4815292/94‑1 (MQ=60)
                                                        GTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCT                                       <  SRR4302543.5869568/94‑1 (MQ=60)
                                                        GTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCT                                       >  SRR4302543.6435024/1‑94 (MQ=60)
                                                        GTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCT                                       >  SRR4302543.3557694/1‑94 (MQ=60)
                                                          AGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTA                                     >  SRR4302543.99567/1‑94 (MQ=60)
                                                          AGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTA                                     >  SRR4302543.1869661/1‑94 (MQ=60)
                                                          AGCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTA                                     <  SRR4302543.6878319/94‑1 (MQ=60)
                                                           GCCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAA                                    <  SRR4302543.5768493/94‑1 (MQ=60)
                                                            CCACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAG                                   >  SRR4302543.2987698/1‑94 (MQ=60)
                                                             CACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGG                                  <  SRR4302543.6788840/94‑1 (MQ=60)
                                                              ACTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGT                                 >  SRR4302543.1365500/1‑94 (MQ=60)
                                                               CTGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTT                                >  SRR4302543.413586/1‑94 (MQ=60)
                                                                TGTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTT                               <  SRR4302543.3528974/94‑1 (MQ=60)
                                                                 GTGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTG                              <  SRR4302543.4729714/94‑1 (MQ=60)
                                                                  TGCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGC                             >  SRR4302543.4659215/1‑94 (MQ=60)
                                                                  TGCCATTTGCGGATACTCCCTCAACACCGACTCAAGGCTCACTGGTTCTGGCTCGTCGAGAGGATGCACACCTTGCGGATCGCTTAAGGTTTGC                             <  SRR4302543.4793578/94‑1 (MQ=32)
                                                                   GCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCA                            <  SRR4302543.117808/94‑1 (MQ=60)
                                                                   GCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCA                            >  SRR4302543.6765979/1‑94 (MQ=60)
                                                                   GCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCA                            <  SRR4302543.574774/94‑1 (MQ=60)
                                                                   GCCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCA                            >  SRR4302543.7496299/1‑94 (MQ=60)
                                                                    gctgTTGCGGATACTCCCTCAACCCCGACTCCCACACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAG                           <  SRR4302543.803078/90‑1 (MQ=60)
                                                                    CCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTACGGTTTGCAG                           >  SRR4302543.2916834/1‑94 (MQ=60)
                                                                    CCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAG                           <  SRR4302543.2246829/94‑1 (MQ=60)
                                                                    CCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAG                           >  SRR4302543.615495/1‑94 (MQ=60)
                                                                    CCATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGAAG                           >  SRR4302543.5077627/1‑94 (MQ=60)
                                                                     CATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGC                          >  SRR4302543.4165902/1‑94 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         >  SRR4302543.6350797/1‑94 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         >  SRR4302543.2862985/1‑94 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         >  SRR4302543.2422572/1‑94 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         >  SRR4302543.7526226/1‑94 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         <  SRR4302543.3912974/94‑1 (MQ=60)
                                                                      ATTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCC                         >  SRR4302543.61082/1‑94 (MQ=60)
                                                                       TTTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCC                        >  SRR4302543.2188432/1‑94 (MQ=60)
                                                                        TTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCC                       <  SRR4302543.5982529/94‑1 (MQ=60)
                                                                        TTGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCC                       <  SRR4302543.3748369/94‑1 (MQ=60)
                                                                         TGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCA                      <  SRR4302543.2095556/94‑1 (MQ=60)
                                                                         TGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCA                      <  SRR4302543.6120145/94‑1 (MQ=60)
                                                                         TGCGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCA                      <  SRR4302543.694035/94‑1 (MQ=60)
                                                                           CGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGC                    <  SRR4302543.852253/94‑1 (MQ=60)
                                                                           CGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGC                    >  SRR4302543.5952863/1‑94 (MQ=60)
                                                                           CGGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGC                    <  SRR4302543.4948671/94‑1 (MQ=60)
                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                   >  SRR4302543.6984583/1‑94 (MQ=60)
                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                   <  SRR4302543.137935/94‑1 (MQ=60)
                                                                            GGATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCG                   <  SRR4302543.4705675/94‑1 (MQ=60)
                                                                              ATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCC                 <  SRR4302543.1137698/94‑1 (MQ=60)
                                                                              ATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCC                 <  SRR4302543.743641/94‑1 (MQ=60)
                                                                              ATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCC                 >  SRR4302543.5909612/1‑94 (MQ=60)
                                                                              ATACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCC                 >  SRR4302543.1632765/1‑94 (MQ=60)
                                                                              ATACTCCCTCAACACCGACTCACGCACCACTGCTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCC                 >  SRR4302543.2439603/1‑94 (MQ=60)
                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC               >  SRR4302543.7251637/1‑94 (MQ=60)
                                                                                ACTCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGC               >  SRR4302543.4207615/1‑94 (MQ=60)
                                                                                  TCCCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTA             >  SRR4302543.461795/1‑94 (MQ=60)
                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC           <  SRR4302543.6803958/94‑1 (MQ=60)
                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC           <  SRR4302543.154743/94‑1 (MQ=60)
                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC           >  SRR4302543.908061/1‑94 (MQ=60)
                                                                                    CCTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACC           <  SRR4302543.4147575/94‑1 (MQ=60)
                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          >  SRR4302543.5210875/1‑94 (MQ=60)
                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          <  SRR4302543.4260372/94‑1 (MQ=60)
                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          <  SRR4302543.4460737/94‑1 (MQ=60)
                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          >  SRR4302543.934337/1‑94 (MQ=60)
                                                                                     CTCAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCG          <  SRR4302543.2687445/94‑1 (MQ=60)
                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG        >  SRR4302543.6716375/1‑94 (MQ=60)
                                                                                       CAACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGG        <  SRR4302543.5950716/94‑1 (MQ=60)
                                                                                        AACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC       >  SRR4302543.5856658/1‑94 (MQ=60)
                                                                                        AACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC       >  SRR4302543.6686150/1‑94 (MQ=60)
                                                                                        AACACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGC       <  SRR4302543.6285981/94‑1 (MQ=60)
                                                                                          CACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCG     >  SRR4302543.1907132/1‑94 (MQ=60)
                                                                                          CACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCG     >  SRR4302543.938261/1‑94 (MQ=60)
                                                                                           ACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGT    >  SRR4302543.566009/1‑94 (MQ=60)
                                                                                           ACCGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGT    >  SRR4302543.2131781/1‑94 (MQ=60)
                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  <  SRR4302543.7671265/94‑1 (MQ=60)
                                                                                             CGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  <  SRR4302543.7527974/94‑1 (MQ=60)
                                                                                             |                                                                                             
AATGCTGGCATTCAGTTGCTGCAATGTTTTTGCATCGAGGCTGGCGAAGACTGGCTGTAGCCACTGTGCCATTTGCGGATACTCCCTCAACACGGACTCACGCACCACTGGTGCAGGCGCGTAGATAGGTTGCACACCTTGCGGATCGCTTAAGGTTTGCAGCCCCAGCGCCGCTACCGGGCCGTCA  >  NC_000913/2216653‑2216839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑