Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,254,317 T→C T347A (ACG→GCG)  yeiM ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,3170TC100.0% 686.0 / NA 221T347A (ACG→GCG) yeiMputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (109/112);  total (109/112)

Only 100 of 236 total aligned reads displayed.

AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT  >  NC_000913/2254227‑2254406
                                                                                           |                                                                                         
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑gcgcgc                                                                                         >  2:1858628/1‑93 (MQ=255)
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑gcgcgc                                                                                         <  2:5766271/93‑1 (MQ=255)
aaaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑gcgcgc                                                                                         >  2:6773395/1‑93 (MQ=255)
 aaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCgcc                                                                                        >  1:5961071/1‑93 (MQ=255)
 aaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCgcc                                                                                        >  1:3995890/1‑93 (MQ=255)
  aaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGGCCAAAGGAGTAAATCGCATTGGTTTTACCTTCAA‑gcgcgcgc                                                                                       >  1:4339551/1‑91 (MQ=255)
        ccAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGtt                                                                                 >  1:566152/1‑93 (MQ=255)
         cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGttt                                                                                >  2:5750168/1‑93 (MQ=255)
          aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGtttc                                                                               >  2:4495281/1‑92 (MQ=255)
          aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTg                                                                               >  1:2105925/1‑93 (MQ=255)
           aCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGc                                                                              >  1:7780084/1‑93 (MQ=255)
              acaacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAgg                                                                           <  1:5280617/93‑1 (MQ=255)
                aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCC‑GCGCGCCGCCCGTTTGAAGGTa                                                                         >  1:1006874/1‑93 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTAt                                                                        >  1:79675/1‑93 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTAt                                                                        <  2:4216620/93‑1 (MQ=255)
                 acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGGTTGCAGGTAt                                                                        >  2:4691144/1‑93 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATggg                                                                     >  1:6486654/1‑93 (MQ=255)
                    ccGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATggg                                                                     <  2:5538028/93‑1 (MQ=255)
                    cagATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATggg                                                                     <  2:189757/91‑1 (MQ=255)
                     cGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATgggg                                                                    >  2:3670740/1‑93 (MQ=255)
                      gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGga                                                                   <  2:1255378/93‑1 (MQ=255)
                        tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGgaga                                                                 >  2:1278852/1‑93 (MQ=255)
                              cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                           <  2:3559338/93‑1 (MQ=255)
                               aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          >  2:6386841/1‑93 (MQ=255)
                               aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          <  2:3092999/93‑1 (MQ=255)
                               aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          >  1:1731861/1‑93 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         <  2:7703071/93‑1 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         >  2:7833813/1‑93 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         <  1:3273898/93‑1 (MQ=255)
                                aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         <  1:2827477/93‑1 (MQ=255)
                                 aGTTAGCAAAACCACAAGGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGt                                                        <  1:7016880/93‑1 (MQ=255)
                                 aGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgga                                                        >  2:2592910/1‑92 (MQ=255)
                                 aGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGt                                                        <  2:5777384/93‑1 (MQ=255)
                                 aGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGt                                                        <  1:5934493/93‑1 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       <  2:4313732/93‑1 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       <  1:686765/93‑1 (MQ=255)
                                  gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGGTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       >  2:3436584/1‑93 (MQ=255)
                                   ttAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                      <  2:3415563/93‑1 (MQ=255)
                                       cAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcga                                                  <  2:1542973/93‑1 (MQ=255)
                                       cAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcga                                                  <  2:3248757/93‑1 (MQ=255)
                                        aaaaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgac                                                 <  1:7477179/93‑1 (MQ=255)
                                          aaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgacga                                               >  1:7387725/1‑93 (MQ=255)
                                          aaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgacga                                               >  1:2624242/1‑93 (MQ=255)
                                          aaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgacga                                               >  2:5330524/1‑93 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                              <  2:3120023/93‑1 (MQ=255)
                                           aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                              <  2:6952794/93‑1 (MQ=255)
                                            ccACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAt                                             >  2:3656585/1‑93 (MQ=255)
                                            ccACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAt                                             <  2:5477795/93‑1 (MQ=255)
                                             cacaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAtt                                            >  1:6249451/1‑93 (MQ=255)
                                             cacaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCACGTCTGGGGAGAAACTCAGGTAAGCGACGAAtt                                            >  1:6967126/1‑93 (MQ=255)
                                                  aGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTa                                       <  1:2681773/93‑1 (MQ=255)
                                                   gcgcAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTaa                                      >  2:5535664/1‑93 (MQ=255)
                                                    gggaaaaGAGAATATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGCTAAt                                     <  2:5049746/90‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    >  1:5063996/1‑93 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    <  2:6784322/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    <  1:583343/93‑1 (MQ=255)
                                                     gcAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    >  2:300671/1‑93 (MQ=255)
                                                       aaaGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                  >  2:2517905/1‑93 (MQ=255)
                                                        aaGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                 >  1:7838006/1‑93 (MQ=255)
                                                         aGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCa                                >  1:1939149/1‑93 (MQ=255)
                                                            agaTAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGtt                             >  1:3782503/1‑93 (MQ=255)
                                                            agaTAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGtt                             <  2:6792206/93‑1 (MQ=255)
                                                            agaTAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGtt                             <  2:3383924/93‑1 (MQ=255)
                                                             gaTAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                            >  1:5113814/1‑93 (MQ=255)
                                                              aTAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  2:3848092/93‑1 (MQ=255)
                                                              aTAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAATCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  1:6009294/93‑1 (MQ=255)
                                                              aTAATCGCAATGGTTTTCACTTCCA‑GCGCGCAACCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  2:7903442/93‑1 (MQ=255)
                                                               tAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCt                          >  1:5628417/1‑93 (MQ=255)
                                                               tAATCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCt                          >  1:6314410/1‑93 (MQ=255)
                                                                 aTCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                        >  2:2828239/1‑93 (MQ=255)
                                                                 aTCGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                        >  1:3573331/1‑93 (MQ=255)
                                                                   cGCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGccc                      <  1:6682791/93‑1 (MQ=255)
                                                                    gCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTGAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCa                     >  2:2328287/1‑93 (MQ=255)
                                                                    gCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCa                     >  1:1295750/1‑93 (MQ=255)
                                                                    gCAATGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCa                     <  1:2672431/93‑1 (MQ=255)
                                                                      aaTGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   <  2:2059129/93‑1 (MQ=255)
                                                                      aaTGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   <  1:7107385/93‑1 (MQ=255)
                                                                       aTGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  >  2:1778756/1‑93 (MQ=255)
                                                                       aTGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  <  1:1371248/93‑1 (MQ=255)
                                                                        tGGTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 <  1:3550541/93‑1 (MQ=255)
                                                                        gggTTTTCA‑TTCCAGGCGCGCCGCCCGTTGGC‑GGGAAGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg                <  1:233865/92‑1 (MQ=255)
                                                                         ggTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg                <  1:1534445/93‑1 (MQ=255)
                                                                         ggTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg                <  1:1676310/93‑1 (MQ=255)
                                                                         ggTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg                <  1:3592356/93‑1 (MQ=255)
                                                                          gTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAgg               >  1:5458086/1‑93 (MQ=255)
                                                                          gTTTTCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAgg               >  1:3125328/1‑93 (MQ=255)
                                                                           ttttCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGc              <  1:2706795/93‑1 (MQ=255)
                                                                            tttCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCt             >  1:6326475/1‑93 (MQ=255)
                                                                             ttCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTa            <  2:4980771/93‑1 (MQ=255)
                                                                              tCACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAc           >  2:2926506/1‑93 (MQ=255)
                                                                               cACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAcc          <  1:4682450/93‑1 (MQ=255)
                                                                               cACTTCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAcc          >  2:7331132/1‑93 (MQ=255)
                                                                                   tCCA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCa      >  2:7619434/1‑93 (MQ=255)
                                                                                    ccA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCaa     <  2:1054554/93‑1 (MQ=255)
                                                                                     cA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAg    <  1:4924747/93‑1 (MQ=255)
                                                                                     cA‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATCCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAg    >  2:6230887/1‑93 (MQ=255)
                                                                                      a‑GCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGa   <  2:102167/93‑1 (MQ=255)
                                                                                        gcgcgcCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAt  >  2:7562230/1‑93 (MQ=255)
                                                                                        gcgcgcCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAt  <  2:7122824/93‑1 (MQ=255)
                                                                                        gcgcgcCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAt  <  2:2624039/93‑1 (MQ=255)
                                                                                           |                                                                                         
AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCA‑GCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT  >  NC_000913/2254227‑2254406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTG  >  NC_000913/2254224‑2254408
                                                                                             |                                                                                           
acaAAAATGCGCGAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGC                                                                                             <  SRR4302543.3107479/91‑1 (MQ=60)
 CGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCG                                                                                            >  SRR4302543.2081061/1‑94 (MQ=60)
   AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCC                                                                                          >  SRR4302543.4031498/1‑94 (MQ=60)
   AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCC                                                                                          >  SRR4302543.6015650/1‑94 (MQ=60)
    AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCG                                                                                         <  SRR4302543.4993744/94‑1 (MQ=60)
    AAATGCACCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCG                                                                                         <  SRR4302543.7050851/94‑1 (MQ=60)
        GCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCG                                                                                     >  SRR4302543.2317132/1‑94 (MQ=60)
         CGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGT                                                                                    >  SRR4302543.959200/1‑94 (MQ=60)
          GCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTT                                                                                   >  SRR4302543.571336/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                 >  SRR4302543.1367868/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                 >  SRR4302543.2124767/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                 <  SRR4302543.6332173/94‑1 (MQ=60)
             AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                >  SRR4302543.5707900/1‑94 (MQ=60)
             AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                >  SRR4302543.7853446/1‑94 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                            >  SRR4302543.3611064/1‑94 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                            <  SRR4302543.5328265/94‑1 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                            <  SRR4302543.1483188/94‑1 (MQ=60)
                  CAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCCGCGCGCCGCCCGTTTGAAGGTA                                                                           >  SRR4302543.1015780/1‑94 (MQ=60)
                  CAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTA                                                                           <  SRR4302543.6506259/94‑1 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                          >  SRR4302543.80417/1‑94 (MQ=60)
                     CACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGG                                                                        <  SRR4302543.7902708/94‑1 (MQ=60)
                      ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGG                                                                       <  SRR4302543.3737846/94‑1 (MQ=60)
                      ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGG                                                                       >  SRR4302543.6547768/1‑94 (MQ=60)
                      ACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGG                                                                       <  SRR4302543.5960448/94‑1 (MQ=60)
                         GATAGAACCAAAGTTAGCAAAACCACAAAGCGCGAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAG                                                                    >  SRR4302543.5396071/1‑94 (MQ=60)
                         GATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAG                                                                    <  SRR4302543.4856672/94‑1 (MQ=60)
                           TAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAA                                                                  >  SRR4302543.6005159/1‑94 (MQ=60)
                            AGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAA                                                                 >  SRR4302543.6197480/1‑94 (MQ=60)
                               ACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTC                                                              >  SRR4302543.1234414/1‑94 (MQ=60)
                                CCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCA                                                             <  SRR4302543.3040486/94‑1 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            >  SRR4302543.7135692/1‑94 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            <  SRR4302543.4386567/94‑1 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            >  SRR4302543.7825438/1‑94 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            >  SRR4302543.2713542/1‑94 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            >  SRR4302543.1747301/1‑94 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            >  SRR4302543.6541368/1‑94 (MQ=60)
                                 CAAAGATAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                            <  SRR4302543.360527/94‑1 (MQ=60)
                                  AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGG                                                           >  SRR4302543.1575852/1‑94 (MQ=60)
                                   AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                          <  SRR4302543.2853343/94‑1 (MQ=60)
                                   AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                          <  SRR4302543.3303472/94‑1 (MQ=60)
                                    AGTTAGCAAAACCACAAGGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTA                                                         <  SRR4302543.7083941/94‑1 (MQ=60)
                                    AGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTA                                                         <  SRR4302543.5988771/94‑1 (MQ=60)
                                    AGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTA                                                         <  SRR4302543.5576257/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                        <  SRR4302543.693084/94‑1 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                        >  SRR4302543.5157920/1‑94 (MQ=60)
                                      TTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAG                                                       <  SRR4302543.6837384/94‑1 (MQ=60)
                                        AGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCG                                                     <  SRR4302543.6628235/94‑1 (MQ=60)
                                        AGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCG                                                     <  SRR4302543.3594121/94‑1 (MQ=60)
                                           AAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACG                                                  <  SRR4302543.7548760/94‑1 (MQ=60)
                                            AAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGA                                                 >  SRR4302543.7458707/1‑94 (MQ=60)
                                            AAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGA                                                 >  SRR4302543.2648001/1‑94 (MQ=60)
                                               CCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATT                                              >  SRR4302543.3517591/1‑94 (MQ=60)
                                               CCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATT                                              >  SRR4302543.6308405/1‑94 (MQ=60)
                                               CCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCACGTCTGGGGAGAAACTCAGGTAAGCGACGAATT                                              >  SRR4302543.7032691/1‑94 (MQ=60)
                                                CACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTC                                             <  SRR4302543.1735430/94‑1 (MQ=60)
                                                CACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTC                                             <  SRR4302543.4757811/94‑1 (MQ=60)
                                                  CAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGT                                           <  SRR4302543.5120707/94‑1 (MQ=60)
                                                     AGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAA                                        <  SRR4302543.2706077/94‑1 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                      <  SRR4302543.994836/94‑1 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                      >  SRR4302543.5109986/1‑94 (MQ=60)
                                                        GCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCG                                     <  SRR4302543.588671/94‑1 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                   >  SRR4302543.6119920/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                   >  SRR4302543.7911774/1‑94 (MQ=60)
                                                           AAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCA                                  >  SRR4302543.1956574/1‑94 (MQ=60)
                                                           AAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCA                                  <  SRR4302543.5294881/94‑1 (MQ=60)
                                                            AGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAG                                 <  SRR4302543.7523027/94‑1 (MQ=60)
                                                            AGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAG                                 <  SRR4302543.2691678/94‑1 (MQ=60)
                                                             GGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGT                                >  SRR4302543.2698396/1‑94 (MQ=60)
                                                              GAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTT                               >  SRR4302543.3815824/1‑94 (MQ=60)
                                                               AGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTT                              >  SRR4302543.5160076/1‑94 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                            >  SRR4302543.6373802/1‑94 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                            >  SRR4302543.5679708/1‑94 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAATCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                            <  SRR4302543.6064678/94‑1 (MQ=60)
                                                                   AATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGC                          >  SRR4302543.3604918/1‑94 (MQ=60)
                                                                      CGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCA                       <  SRR4302543.6745878/94‑1 (MQ=60)
                                                                      CGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCA                       >  SRR4302543.1307277/1‑94 (MQ=60)
                                                                      CGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCCGCCCA                       >  SRR4302543.638008/1‑94 (MQ=60)
                                                                       GCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAA                      <  SRR4302543.2696663/94‑1 (MQ=60)
                                                                       GCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAA                      >  SRR4302543.2416273/1‑94 (MQ=60)
                                                                       GCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAA                      >  SRR4302543.798618/1‑94 (MQ=60)
                                                                        CAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAT                     >  SRR4302543.5972112/1‑94 (MQ=60)
                                                                         AATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATC                    <  SRR4302543.7176428/94‑1 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCTGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                   <  SRR4302543.3911653/94‑1 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                   <  SRR4302543.1383566/94‑1 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                   <  SRR4302543.7158985/94‑1 (MQ=60)
                                                                           TGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAG                  <  SRR4302543.3582038/94‑1 (MQ=60)
                                                                           TGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAG                  <  SRR4302543.6964221/94‑1 (MQ=60)
                                                                           TGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAG                  <  SRR4302543.2362943/94‑1 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                 >  SRR4302543.3153439/1‑94 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                 <  SRR4302543.1691336/94‑1 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                 <  SRR4302543.1548190/94‑1 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                 <  SRR4302543.3624067/94‑1 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                 >  SRR4302543.5507559/1‑94 (MQ=60)
                                                                             GTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGC                >  SRR4302543.1818633/1‑94 (MQ=60)
                                                                             GTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGC                >  SRR4302543.2907912/1‑94 (MQ=60)
                                                                             GTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGC                <  SRR4302543.7243270/94‑1 (MQ=60)
                                                                             GTTTTCACTACCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGC                >  SRR4302543.77502/1‑94 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT               <  SRR4302543.2731361/94‑1 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT               <  SRR4302543.1484547/94‑1 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT               >  SRR4302543.6385974/1‑94 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT               <  SRR4302543.576853/94‑1 (MQ=60)
                                                                                 TCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATAGCCAGTTTCTGCCCAATCAGGCTACC            >  SRR4302543.1922163/1‑94 (MQ=60)
                                                                                  CACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCC           <  SRR4302543.2080170/94‑1 (MQ=60)
                                                                                  CACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCC           <  SRR4302543.4725151/94‑1 (MQ=60)
                                                                                   tcttgCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGGCGAATTCGTTAATCGCAAGTTTCTGCCCAATCAGGCTACCCG          <  SRR4302543.2725555/89‑1 (MQ=60)
                                                                                   ACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCG          <  SRR4302543.6788366/94‑1 (MQ=60)
                                                                                    CTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGC         >  SRR4302543.6035641/1‑94 (MQ=60)
                                                                                     TTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCA        <  SRR4302543.31664/94‑1 (MQ=60)
                                                                                       CCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAG      <  SRR4302543.5627565/94‑1 (MQ=60)
                                                                                        CAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA     <  SRR4302543.3853022/94‑1 (MQ=60)
                                                                                        CAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA     >  SRR4302543.4275185/1‑94 (MQ=60)
                                                                                        CAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGA     <  SRR4302543.4969611/94‑1 (MQ=60)
                                                                                         AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT    <  SRR4302543.2814475/94‑1 (MQ=60)
                                                                                           CGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTG  >  SRR4302543.6678436/1‑94 (MQ=60)
                                                                                             |                                                                                           
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTG  >  NC_000913/2254224‑2254408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑