Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,321,092	G→A	A403T (GCC→ACC)	atoC →	ornithine decarboxylase antizyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,321,092	0	G	A	99.2%	848.0 / ‑4.4	253	A403T (GCC→ACC)	atoC	ornithine decarboxylase antizyme
Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (135/114); total (135/118)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

Only 100 of 262 total aligned reads displayed.

GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC  >  NC_000913/2321002‑2321181

gtcgtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAcc                                                                                         <  1:4823212/93‑1 (MQ=255)

gtcgtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAcc                                                                                         >  1:1845289/1‑93 (MQ=255)

 tcgtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAccc                                                                                        >  1:2648849/1‑93 (MQ=255)

  cgtGATGAATTCAGGCCCGATCAGTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAcccc                                                                                       <  2:5368890/93‑1 (MQ=255)

   gtGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTCCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCt                                                                                      <  2:7747642/93‑1 (MQ=255)

    tgatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTg                                                                                     >  1:1971645/1‑93 (MQ=255)

    tgatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGGAATGCTGGCGAGGTAAAAACAACCCGTg                                                                                     >  2:6694833/1‑93 (MQ=255)

     gatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGt                                                                                    >  2:6782565/1‑93 (MQ=255)

     gatgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGt                                                                                    <  2:7733846/93‑1 (MQ=255)

     gatgaATTCAGGCCCGATCATTTTTTCTCAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGt                                                                                    >  1:4761998/1‑93 (MQ=255)

      atgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTc                                                                                   <  1:7675523/93‑1 (MQ=255)

       tgaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCg                                                                                  >  2:2012802/1‑93 (MQ=255)

       tgaATTCAGCCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCg                                                                                  <  2:3054110/93‑1 (MQ=255)

        gaATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCgg                                                                                 >  1:7566317/1‑93 (MQ=255)

          aTTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTg                                                                               <  1:2149324/93‑1 (MQ=255)

           ttCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTgg                                                                              >  1:7112567/1‑92 (MQ=255)

           ttCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGa                                                                              >  1:1364306/1‑93 (MQ=255)

               ggCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGt                                                                          >  2:5455048/1‑93 (MQ=255)

               ggCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGt                                                                          >  2:5803422/1‑93 (MQ=255)

               ggCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGt                                                                          <  2:2823084/93‑1 (MQ=255)

                gCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTa                                                                         >  2:5283949/1‑93 (MQ=255)

                 cccGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTaa                                                                        <  2:5325589/93‑1 (MQ=255)

                  ccGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAt                                                                       <  2:6791815/93‑1 (MQ=255)

                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  2:6021881/1‑93 (MQ=255)

                   cGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  1:1571041/1‑93 (MQ=255)

                   cGATCATTTTTTCTGAGGATCTTCCGACACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAtt                                                                      >  2:2625707/1‑93 (MQ=255)

                    gATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAttt                                                                     >  2:5069825/1‑93 (MQ=255)

                     aTCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTa                                                                    >  2:1046450/1‑93 (MQ=255)

                     aTCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTa                                                                    >  2:251079/1‑93 (MQ=255)

                       cATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTaaa                                                                  >  1:7481332/1‑93 (MQ=255)

                        aTTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTaaaa                                                                 <  1:1181906/93‑1 (MQ=255)

                        aTTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTaaaa                                                                 >  1:2727280/1‑93 (MQ=255)

                         ttttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAag                                                                >  2:407809/1‑93 (MQ=255)

                         ttttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAag                                                                >  1:5058595/1‑93 (MQ=255)

                         ttttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAag                                                                >  2:1748507/1‑93 (MQ=255)

                          tttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAaga                                                               <  1:2525554/93‑1 (MQ=255)

                          tttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAaga                                                               <  1:7815181/93‑1 (MQ=255)

                           ttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAagag                                                              >  2:4106049/1‑93 (MQ=255)

                           ttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAagag                                                              <  2:2213283/93‑1 (MQ=255)

                           ttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAagag                                                              <  2:5214344/93‑1 (MQ=255)

                           ttttCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAagag                                                              <  2:7323298/93‑1 (MQ=255)

                               cTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGaaa                                                          <  2:6425413/93‑1 (MQ=255)

                               cTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGaaa                                                          >  2:3284374/1‑93 (MQ=255)

                                tGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAAt                                                         >  2:197162/1‑93 (MQ=255)

                                 gaggagCTTCCGCCACAGATCCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAAtt                                                        <  1:5861460/93‑1 (MQ=255)

                                   ggATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTaa                                                      >  1:6862123/1‑93 (MQ=255)

                                    gATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTaaa                                                     <  1:422899/93‑1 (MQ=255)

                                    gATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTaaa                                                     <  2:3673296/93‑1 (MQ=255)

                                     aTCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAc                                                    >  2:2468305/1‑93 (MQ=255)

                                      tCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAcg                                                   >  1:7002385/1‑93 (MQ=255)

                                          ccGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTc                                               <  2:463850/93‑1 (MQ=255)

                                             ccACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaa                                            <  1:3761559/93‑1 (MQ=255)

                                             ccACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaa                                            >  1:5045516/1‑93 (MQ=255)

                                              cacaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaa                                           >  1:5325578/1‑93 (MQ=255)

                                               acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaa                                          >  2:4346619/1‑93 (MQ=255)

                                               acaGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaa                                          >  2:3841254/1‑93 (MQ=255)

                                                caGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaaa                                         >  1:4493025/1‑93 (MQ=255)

                                                caGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGaaaaa                                         >  2:4170844/1‑93 (MQ=255)

                                                 aGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAc                                        >  1:2104975/1‑93 (MQ=255)

                                                 aGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAAc                                        <  2:4258352/93‑1 (MQ=255)

                                                  gATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACg                                       >  1:6592855/1‑93 (MQ=255)

                                                  gATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACg                                       >  1:6798438/1‑93 (MQ=255)

                                                  gATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACg                                       >  2:3745058/1‑93 (MQ=255)

                                                   aTTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGc                                      >  1:2947393/1‑93 (MQ=255)

                                                    ttCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGca                                     <  2:2508226/93‑1 (MQ=255)

                                                    ttCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGca                                     >  1:5731285/1‑93 (MQ=255)

                                                      cGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACACCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatc                                   <  1:559173/93‑1 (MQ=255)

                                                      cGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGcatc                                   <  1:1343994/93‑1 (MQ=255)

                                                         cAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCAtt                                <  1:3748825/93‑1 (MQ=255)

                                                         cAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAAGCGTCGAAAACAGCATCAtt                                >  2:4647128/1‑93 (MQ=255)

                                                          aGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTa                               <  2:1847876/93‑1 (MQ=255)

                                                           gCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTAt                              >  2:4918812/1‑93 (MQ=255)

                                                            ccAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATg                             <  1:1245434/93‑1 (MQ=255)

                                                             cAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATgg                            >  1:5433027/1‑93 (MQ=255)

                                                              aGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGa                           >  1:6163091/1‑93 (MQ=255)

                                                               gTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGaa                          <  1:647035/93‑1 (MQ=255)

                                                               gTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGaa                          <  1:4948207/93‑1 (MQ=255)

                                                                tCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAg                         <  2:1511047/93‑1 (MQ=255)

                                                                tCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAg                         >  1:832125/1‑93 (MQ=255)

                                                                 cTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGt                        >  1:4316216/1‑93 (MQ=255)

                                                                 cTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGt                        <  2:724624/93‑1 (MQ=255)

                                                                  tGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTg                       >  1:3158395/1‑93 (MQ=255)

                                                                  tGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTg                       >  1:5784245/1‑93 (MQ=255)

                                                                   gTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGc                      <  2:7917854/93‑1 (MQ=255)

                                                                     aaTGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTg                    <  2:2421059/93‑1 (MQ=255)

                                                                      aTGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTgg                   >  2:5987309/1‑93 (MQ=255)

                                                                       tGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGa                  <  2:3398450/93‑1 (MQ=255)

                                                                         cTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac                <  2:5880575/93‑1 (MQ=255)

                                                                         cTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac                <  2:5538464/93‑1 (MQ=255)

                                                                         cTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaac                >  1:4644828/1‑93 (MQ=255)

                                                                          tGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaaca               >  1:6278183/1‑93 (MQ=255)

                                                                           ggCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaacaa              >  1:6030248/1‑93 (MQ=255)

                                                                            gCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGaacaac             >  2:4988777/1‑93 (MQ=255)

                                                                              gAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAAcaacaa           <  2:6194414/93‑1 (MQ=255)

                                                                                 gTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaagaa        <  1:5958914/93‑1 (MQ=255)

                                                                                 gTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACaagaa        <  2:7126685/93‑1 (MQ=255)

                                                                                   aaaaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAgg      <  2:3586577/93‑1 (MQ=255)

                                                                                    aaaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGa     <  2:6567247/93‑1 (MQ=255)

                                                                                    aaaaCAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGa     <  1:7277841/93‑1 (MQ=255)

                                                                                      aacaacCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGaaa   <  1:3209149/93‑1 (MQ=255)

GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAAAACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC  >  NC_000913/2321002‑2321181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAA  >  NC_000913/2320999‑2321185

GCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAA                                                                                                >  SRR4302543.4160411/1‑94 (MQ=60)

GCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAA                                                                                                <  SRR4302543.5655093/94‑1 (MQ=60)

GCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAA                                                                                                <  SRR4302543.793011/94‑1 (MQ=60)

GCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGAAAAAACAA                                                                                                >  SRR4302543.1664721/1‑94 (MQ=60)

GCAGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAA                                                                                                <  SRR4302543.4672706/94‑1 (MQ=60)

 CCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAAC                                                                                               >  SRR4302543.6547636/1‑94 (MQ=60)

  CGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACC                                                                                              >  SRR4302543.1861884/1‑94 (MQ=60)

   GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                             >  SRR4302543.5277550/1‑94 (MQ=60)

   GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                             >  SRR4302543.5374691/1‑94 (MQ=60)

   GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                             >  SRR4302543.2672860/1‑94 (MQ=60)

   GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                             <  SRR4302543.4867231/94‑1 (MQ=60)

   GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                             >  SRR4302543.1970256/1‑94 (MQ=60)

   GTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCC                                                                                             <  SRR4302543.1492909/94‑1 (MQ=60)

     CGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCT                                                                                           <  SRR4302543.1754722/94‑1 (MQ=60)

     CGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCT                                                                                           <  SRR4302543.6477084/94‑1 (MQ=60)

     CGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCT                                                                                           >  SRR4302543.2143008/1‑94 (MQ=60)

      GTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTG                                                                                          >  SRR4302543.1753450/1‑94 (MQ=60)

      GTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTG                                                                                          >  SRR4302543.6901082/1‑94 (MQ=60)

      GTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTG                                                                                          >  SRR4302543.1989315/1‑94 (MQ=60)

      GTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTG                                                                                          >  SRR4302543.6333937/1‑94 (MQ=60)

       TGATGAATTCAGGCCCGATCATTTTTTCTCAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGT                                                                                         >  SRR4302543.4805510/1‑94 (MQ=60)

         ATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCG                                                                                       <  SRR4302543.7748241/94‑1 (MQ=60)

         ATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCG                                                                                       >  SRR4302543.7048432/1‑94 (MQ=60)

          TGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGG                                                                                      >  SRR4302543.7638316/1‑94 (MQ=60)

          TGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGG                                                                                      <  SRR4302543.3030561/94‑1 (MQ=60)

          TGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCCACGTAAAAACAACCCCTGTCGG                                                                                      >  SRR4302543.7638454/1‑94 (MQ=60)

           GAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGT                                                                                     <  SRR4302543.78353/94‑1 (MQ=60)

            AATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTG                                                                                    <  SRR4302543.4667847/94‑1 (MQ=60)

             ATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGG                                                                                   >  SRR4302543.7181664/1‑94 (MQ=60)

             ATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGA                                                                                   <  SRR4302543.337443/94‑1 (MQ=60)

             ATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGA                                                                                   >  SRR4302543.7181466/1‑94 (MQ=60)

             ATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGA                                                                                   >  SRR4302543.1376531/1‑94 (MQ=60)

             ATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGA                                                                                   <  SRR4302543.2168568/94‑1 (MQ=60)

              TTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAG                                                                                  >  SRR4302543.2935184/1‑94 (MQ=60)

                CAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCG                                                                                <  SRR4302543.4746364/94‑1 (MQ=60)

                CAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCG                                                                                >  SRR4302543.795540/1‑94 (MQ=60)

                   GCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAA                                                                             >  SRR4302543.4984342/1‑94 (MQ=60)

                    CCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAT                                                                            >  SRR4302543.2030027/1‑94 (MQ=60)

                    CCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAT                                                                            >  SRR4302543.2030026/1‑94 (MQ=60)

                    CCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAAT                                                                            <  SRR4302543.4613268/94‑1 (MQ=60)

                     CCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATT                                                                           >  SRR4302543.1585065/1‑94 (MQ=60)

                      CGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTT                                                                          >  SRR4302543.1502279/1‑94 (MQ=60)

                       GATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTA                                                                         >  SRR4302543.4336587/1‑94 (MQ=60)

                         TCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAA                                                                       >  SRR4302543.7552946/1‑94 (MQ=60)

                          CATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAA                                                                      >  SRR4302543.2752092/1‑94 (MQ=60)

                           ATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAG                                                                     >  SRR4302543.5097552/1‑94 (MQ=60)

                           ATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAG                                                                     >  SRR4302543.5104571/1‑94 (MQ=60)

                           ATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAG                                                                     <  SRR4302543.1192590/94‑1 (MQ=60)

                             TTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAG                                                                   <  SRR4302543.7888826/94‑1 (MQ=60)

                             TTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAG                                                                   <  SRR4302543.2548522/94‑1 (MQ=60)

                                TTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAA                                                                <  SRR4302543.6524272/94‑1 (MQ=60)

                                 TCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAA                                                               <  SRR4302543.713793/94‑1 (MQ=60)

                                  TTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAAT                                                              <  SRR4302543.6690928/94‑1 (MQ=60)

                                   TGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATT                                                             <  SRR4302543.5817048/94‑1 (MQ=60)

                                    GAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTA                                                            >  SRR4302543.748829/1‑94 (MQ=60)

                                    GAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTA                                                            >  SRR4302543.4033619/1‑94 (MQ=60)

                                    GAGGAGCTTCCGCCACAGATCCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTA                                                            <  SRR4302543.5914804/94‑1 (MQ=60)

                                     AGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAA                                                           >  SRR4302543.6926789/1‑94 (MQ=60)

                                       GATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAAC                                                         <  SRR4302543.426854/94‑1 (MQ=60)

                                        atgtgCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                        <  SRR4302543.4789791/89‑1 (MQ=60)

                                        atcttcaacaACAGATTCTTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                        <  SRR4302543.5686924/84‑1 (MQ=60)

                                        ATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                        >  SRR4302543.4742138/1‑94 (MQ=60)

                                        ATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                        >  SRR4302543.7068825/1‑94 (MQ=60)

                                        ATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                        >  SRR4302543.5528034/1‑94 (MQ=60)

                                        ATCTCCCGCCCCAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACG                                                        <  SRR4302543.6030248/94‑1 (MQ=60)

                                          CTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCG                                                      >  SRR4302543.5492110/1‑94 (MQ=60)

                                          CTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCG                                                      >  SRR4302543.4419013/1‑94 (MQ=60)

                                          CTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCG                                                      >  SRR4302543.96729/1‑94 (MQ=60)

                                            TCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTC                                                    <  SRR4302543.7844881/94‑1 (MQ=60)

                                               GCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAA                                                 >  SRR4302543.5091399/1‑94 (MQ=60)

                                                CCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA                                                <  SRR4302543.3794664/94‑1 (MQ=60)

                                                CCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA                                                >  SRR4302543.5373739/1‑94 (MQ=60)

                                                 CACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑A                                              >  SRR4302543.6559362/1‑94 (MQ=60)

                                                 CACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑A                                              <  SRR4302543.4821034/94‑1 (MQ=60)

                                                  ACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AA                                             >  SRR4302543.4533856/1‑94 (MQ=60)

                                                   CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AAC                                            <  SRR4302543.7944968/94‑1 (MQ=60)

                                                   CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AAC                                            >  SRR4302543.2123809/1‑94 (MQ=60)

                                                   CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AAC                                            <  SRR4302543.269964/94‑1 (MQ=60)

                                                   CAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AAC                                            <  SRR4302543.5842413/94‑1 (MQ=60)

                                                    AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAACAAC                                            >  SRR4302543.6862641/1‑94 (MQ=60)

                                                    AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACG                                           >  SRR4302543.5094465/1‑94 (MQ=60)

                                                    AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACG                                           >  SRR4302543.6654971/1‑94 (MQ=60)

                                                    AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACG                                           >  SRR4302543.6862640/1‑94 (MQ=60)

                                                    AGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACG                                           >  SRR4302543.6111802/1‑94 (MQ=60)

                                                     GATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGC                                          >  SRR4302543.2974154/1‑94 (MQ=60)

                                                      ATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCA                                         >  SRR4302543.5783580/1‑94 (MQ=60)

                                                      ATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCA                                         >  SRR4302543.5929215/1‑94 (MQ=60)

                                                       TTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCAT                                        >  SRR4302543.5834995/1‑94 (MQ=60)

                                                        TCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATC                                       <  SRR4302543.3217970/94‑1 (MQ=60)

                                                         CGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACACCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCA                                      <  SRR4302543.564299/94‑1 (MQ=60)

                                                         CGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCA                                      <  SRR4302543.1356002/94‑1 (MQ=60)

                                                          GTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCAT                                     >  SRR4302543.7318446/1‑94 (MQ=60)

                                                            CAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTA                                   >  SRR4302543.1370886/1‑94 (MQ=60)

                                                            CAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTA                                   <  SRR4302543.3781810/94‑1 (MQ=60)

                                                             AGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTAT                                  <  SRR4302543.7626/94‑1 (MQ=60)

                                                             AGCCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTAT                                  >  SRR4302543.6796820/1‑94 (MQ=60)

                                                              GGCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTACCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATG                                 <  SRR4302543.1235638/94‑1 (MQ=60)

                                                               CCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGG                                >  SRR4302543.5482386/1‑94 (MQ=60)

                                                               CCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGG                                <  SRR4302543.3789240/94‑1 (MQ=60)

                                                               CCAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGG                                <  SRR4302543.1256614/94‑1 (MQ=60)

                                                                CAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGA                               >  SRR4302543.6221417/1‑94 (MQ=60)

                                                                CAGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGA                               >  SRR4302543.2960369/1‑94 (MQ=60)

                                                                 AGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAA                              >  SRR4302543.2229755/1‑94 (MQ=60)

                                                                 AGTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAA                              >  SRR4302543.1311294/1‑94 (MQ=60)

                                                                  GTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAG                             >  SRR4302543.839460/1‑94 (MQ=60)

                                                                  GTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAG                             <  SRR4302543.4993267/94‑1 (MQ=60)

                                                                  GTCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAG                             <  SRR4302543.652950/94‑1 (MQ=60)

                                                                   TCTGTAATGCTGGCGAGGTAAAAGCAGCCCCTGTCTGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGT                            <  SRR4302543.2591093/94‑1 (MQ=60)

                                                                   TCTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGT                            >  SRR4302543.4355421/1‑94 (MQ=60)

                                                                    ttctaatgttgtggataTAAAAACAACCCCTGTCGGTGAGCGTAAATTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTG                           <  SRR4302543.4266491/77‑1 (MQ=60)

                                                                    CTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTG                           >  SRR4302543.3186833/1‑94 (MQ=60)

                                                                    CTGTAATGCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTG                           >  SRR4302543.5836880/1‑94 (MQ=60)

                                                                        tactgTGGCGAGGTAAAACCAACCCCTATCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGG                       <  SRR4302543.4039353/89‑1 (MQ=60)

                                                                           GCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAAC                    >  SRR4302543.6161232/1‑94 (MQ=60)

                                                                           GCTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAAC                    >  SRR4302543.4687092/1‑94 (MQ=60)

                                                                            CTGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACA                   >  SRR4302543.6337364/1‑94 (MQ=60)

                                                                             TGGCGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAA                  >  SRR4302543.6086649/1‑94 (MQ=60)

                                                                              tctGAGGTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAAC                 <  SRR4302543.5838080/91‑1 (MQ=60)

                                                                              GGCGAGGTAAAAACAACCCCTGTCGGTGAGAGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAAC                 <  SRR4302543.2317407/94‑1 (MQ=60)

                                                                                    GTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAG           <  SRR4302543.6013457/94‑1 (MQ=60)

                                                                                    GTAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAG           <  SRR4302543.4344107/94‑1 (MQ=60)

                                                                                     TAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGG          <  SRR4302543.133376/94‑1 (MQ=60)

                                                                                     TAAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGG          <  SRR4302543.5016102/94‑1 (MQ=60)

                                                                                      AAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGA         >  SRR4302543.7627739/1‑94 (MQ=60)

                                                                                      AAAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGA         <  SRR4302543.6085184/94‑1 (MQ=60)

                                                                                       AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGTAAGTGCTGGAACAACAAGAAGGAA        >  SRR4302543.4153138/1‑94 (MQ=60)

                                                                                       AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA        <  SRR4302543.7348135/94‑1 (MQ=60)

                                                                                       AAAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAA        >  SRR4302543.3323109/1‑94 (MQ=60)

                                                                                        AAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAA       <  SRR4302543.4940668/94‑1 (MQ=60)

                                                                                        AAACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAA       >  SRR4302543.5248530/1‑94 (MQ=60)

                                                                                         AACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAAC      <  SRR4302543.3238115/94‑1 (MQ=60)

                                                                                          ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC     <  SRR4302543.265984/94‑1 (MQ=60)

                                                                                          ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC     <  SRR4302543.731643/94‑1 (MQ=60)

                                                                                          ACAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACC     <  SRR4302543.3035646/94‑1 (MQ=60)

                                                                                           CAACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCG    >  SRR4302543.6627901/1‑94 (MQ=60)

                                                                                            AACCCCTGTCGGTGAGCGTAATTTAAAAGAGGGAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGA   >  SRR4302543.2773922/1‑94 (MQ=60)

                                                                                            AACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGA   >  SRR4302543.951002/1‑94 (MQ=60)

                                                                                             ACCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAA  >  SRR4302543.6408987/1‑94 (MQ=60)

GCCGTCGTGATGAATTCAGGCCCGATCATTTTTTCTGAGGATCTTCCGCCACAGATTCGTCAGCCAGTCTGTAATGCTGGCGAGGTAAAAACAGCCCCTGTCGGTGAGCGTAATTTAAAAGAGGAAATTAAACGCGTCGAAA‑AACGCATCATTATGGAAGTGCTGGAACAACAAGAAGGAAACCGAA  >  NC_000913/2320999‑2321185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`