Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,078,221 C→T Q478Q (CAG→CAA)  tktA ← transketolase 1 isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,078,2210CT99.7% 1107.0 / ‑5.5 309Q478Q (CAG→CAA) tktAtransketolase 1 isozyme
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (170/138);  total (170/139)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 322 total aligned reads displayed.

CTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC  >  NC_000913/3078131‑3078310
                                                                                          |                                                                                         
 tCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGc                                                                                        >  1:3873991/1‑93 (MQ=255)
   aaCACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTc                                                                                      <  2:2127843/93‑1 (MQ=255)
   aaCACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTc                                                                                      >  2:5454718/1‑93 (MQ=255)
     cacCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCaa                                                                                    >  2:7737304/1‑93 (MQ=255)
      acCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCacc                                                                                   >  2:1615720/1‑91 (MQ=255)
      acCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAAc                                                                                   >  2:700236/1‑93 (MQ=255)
      acCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAAc                                                                                   <  2:3019285/93‑1 (MQ=255)
       ccGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGCCTTGCTCAAcg                                                                                  >  2:3712346/1‑92 (MQ=255)
        cGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCg                                                                                 <  1:4262268/93‑1 (MQ=255)
         gTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCgg                                                                                >  1:5459786/1‑93 (MQ=255)
         gTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCgg                                                                                >  1:1214707/1‑93 (MQ=255)
          tATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGc                                                                               >  1:5387293/1‑93 (MQ=255)
           aTTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCt                                                                              <  2:6200221/93‑1 (MQ=255)
           aTTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCt                                                                              <  2:7679456/93‑1 (MQ=255)
            tttCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTg                                                                             >  1:6164688/1‑93 (MQ=255)
            tttCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTg                                                                             >  2:2872340/1‑93 (MQ=255)
            ttgccACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTg                                                                             <  1:1986553/90‑1 (MQ=255)
              tCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGt                                                                           <  1:549884/93‑1 (MQ=255)
              tCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGt                                                                           <  1:5512473/93‑1 (MQ=255)
                cACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGtaa                                                                         >  1:2754967/1‑91 (MQ=255)
                cACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGa                                                                         <  2:3431885/93‑1 (MQ=255)
                cACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGa                                                                         >  1:6282273/1‑93 (MQ=255)
                 aCGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAg                                                                        >  1:4186628/1‑93 (MQ=255)
                  cgcgACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGt                                                                       <  1:3681189/93‑1 (MQ=255)
                    cgACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCg                                                                     <  1:4786350/93‑1 (MQ=255)
                    cgACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCg                                                                     <  1:2251385/93‑1 (MQ=255)
                     gACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCgg                                                                    >  2:4701155/1‑93 (MQ=255)
                      aCCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCggg                                                                   >  1:3357524/1‑92 (MQ=255)
                        cgcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGgcc                                                                 <  2:132601/93‑1 (MQ=255)
                         gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCggggcg                                                                >  2:3575246/1‑89 (MQ=255)
                         gcgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCg                                                                >  2:4233541/1‑93 (MQ=255)
                          cgGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGt                                                               <  2:2522443/93‑1 (MQ=255)
                           ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                              <  1:6944616/93‑1 (MQ=255)
                           ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                              >  2:6567852/1‑93 (MQ=255)
                           ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                              <  1:1438616/93‑1 (MQ=255)
                           ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                              >  1:1108607/1‑93 (MQ=255)
                           ggATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTc                                                              >  1:5728519/1‑93 (MQ=255)
                            gATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCt                                                             <  2:810871/93‑1 (MQ=255)
                             aTTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCtt                                                            >  1:2490958/1‑93 (MQ=255)
                                  aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                       <  1:236775/93‑1 (MQ=255)
                                  aCCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGccc                                                       <  1:219506/93‑1 (MQ=255)
                                   ccTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCa                                                      <  1:7057306/93‑1 (MQ=255)
                                      ggTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGAc                                                   <  2:1064709/93‑1 (MQ=255)
                                         cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                <  2:6022947/93‑1 (MQ=255)
                                         cacaCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGa                                                <  1:4697817/93‑1 (MQ=255)
                                          acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGGCGTCTTCGCCCAGACAGAt                                               >  1:2888376/1‑93 (MQ=255)
                                          acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGag                                               >  1:3615097/1‑92 (MQ=255)
                                          acacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAt                                               <  1:4967448/93‑1 (MQ=255)
                                           cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                              >  1:1566853/1‑93 (MQ=255)
                                           cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                              <  1:253827/93‑1 (MQ=255)
                                           cacGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATg                                              >  1:7267344/1‑93 (MQ=255)
                                            acGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATgg                                             <  1:2588249/93‑1 (MQ=255)
                                             cGGCCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGCCCGATGGa                                            <  2:3320493/93‑1 (MQ=255)
                                              ggACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCGCCCAGACCGATGGAg                                           >  1:4491460/1‑93 (MQ=255)
                                               gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATggcgg                                          >  2:7186760/1‑90 (MQ=255)
                                               gACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGt                                          <  1:3568759/93‑1 (MQ=255)
                                                   ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                      <  2:2956483/93‑1 (MQ=255)
                                                   ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                      <  2:5663404/93‑1 (MQ=255)
                                                   ccATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTg                                      >  2:7421555/1‑93 (MQ=255)
                                                    cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                     >  2:2243768/1‑93 (MQ=255)
                                                    cATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTgg                                     <  2:5183715/93‑1 (MQ=255)
                                                      tGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgt                                   <  1:7409689/93‑1 (MQ=255)
                                                       gTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtg                                  >  1:3260428/1‑93 (MQ=255)
                                                        tAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGgtgt                                 <  1:6014126/93‑1 (MQ=255)
                                                          gACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCAGTGGGTGTaa                               >  1:1018941/1‑93 (MQ=255)
                                                           aCATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTaaa                              <  1:89769/93‑1 (MQ=255)
                                                            cATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                             <  2:3983896/93‑1 (MQ=255)
                                                            cATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAc                             >  2:4310976/1‑93 (MQ=255)
                                                              tGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCa                           >  2:6428574/1‑93 (MQ=255)
                                                               gtgcGGGGTTACTCGCAGAGAAGCGACTTGCTCATCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                          <  2:1872606/90‑1 (MQ=255)
                                                               gTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCAt                          <  2:2679173/93‑1 (MQ=255)
                                                                 tCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTTGTGGGTGTAAACCATCa                        <  2:5798280/93‑1 (MQ=255)
                                                                  cGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAc                       >  2:3681225/1‑93 (MQ=255)
                                                                   ggggTTACGCGGAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                      <  2:3905908/93‑1 (MQ=255)
                                                                   ggggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAcc                      <  1:3016701/93‑1 (MQ=255)
                                                                    gggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                     <  1:1909594/93‑1 (MQ=255)
                                                                    gggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCt                     >  1:6743775/1‑93 (MQ=255)
                                                                     ggTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTg                    >  2:1519733/1‑93 (MQ=255)
                                                                      gTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                   <  1:7771525/93‑1 (MQ=255)
                                                                      gTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGa                   >  2:5941968/1‑93 (MQ=255)
                                                                              cAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGttt           <  1:4856110/93‑1 (MQ=255)
                                                                               agagAAGCGACTTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGACGTGGGTGTAAACCATCACCTGACGCTGTTtc          >  1:652465/1‑93 (MQ=255)
                                                                                gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca         >  2:6883309/1‑93 (MQ=255)
                                                                                gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca         <  1:5130120/93‑1 (MQ=255)
                                                                                gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca         <  2:5563326/93‑1 (MQ=255)
                                                                                gagaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtca         <  2:1957146/93‑1 (MQ=255)
                                                                                 agaAGCGCCTTGCTCAACCGGGTGGTGAGTCGGGCCGTCTTCCCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcat        <  2:1566088/93‑1 (MQ=255)
                                                                                 agaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcat        <  2:6131921/93‑1 (MQ=255)
                                                                                 agaAGCGACTTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcat        >  1:1306811/1‑93 (MQ=255)
                                                                                  gaAGCGACTTGCTCATCCGGCTGGTGAGTCGGGCCTTCTTCGCCCAGACCGATGGAGTCGTGTGTGTAACCCATCACCTGACGCTGTTtcatc       <  2:3752118/93‑1 (MQ=255)
                                                                                  gaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc       >  2:4658202/1‑93 (MQ=255)
                                                                                  gaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc       <  1:4409859/93‑1 (MQ=255)
                                                                                  gaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc       >  2:7358023/1‑93 (MQ=255)
                                                                                  gaAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatc       <  1:1052375/93‑1 (MQ=255)
                                                                                   aaGCGACTTGTTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca      <  2:7525087/93‑1 (MQ=255)
                                                                                   aaGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCATCTGACGCTGTTtcatca      >  2:4776393/1‑93 (MQ=255)
                                                                                   aaGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatca      <  2:4461084/93‑1 (MQ=255)
                                                                                    aGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTtcatctg     >  2:1381149/1‑91 (MQ=255)
                                                                                     gCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgc    <  2:6643455/93‑1 (MQ=255)
                                                                                       gACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAgcgc  >  2:7808492/1‑93 (MQ=255)
                                                                                          |                                                                                         
CTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGC  >  NC_000913/3078131‑3078310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC  >  NC_000913/3078128‑3078313
                                                                                             |                                                                                            
ACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACT                                                                                              <  SRR4302543.3561349/94‑1 (MQ=60)
 CGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTT                                                                                             >  SRR4302543.4359776/1‑94 (MQ=60)
  GCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTG                                                                                            >  SRR4302543.1029954/1‑94 (MQ=60)
   CTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGC                                                                                           >  SRR4302543.3908192/1‑94 (MQ=60)
    TCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCT                                                                                          >  SRR4302543.5481965/1‑94 (MQ=60)
      AACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCA                                                                                        <  SRR4302543.1112392/94‑1 (MQ=60)
      AACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCA                                                                                        <  SRR4302543.1291121/94‑1 (MQ=60)
        CACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAAC                                                                                      >  SRR4302543.823226/1‑94 (MQ=60)
        CACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAAC                                                                                      >  SRR4302543.823224/1‑94 (MQ=60)
        CACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAAC                                                                                      >  SRR4302543.1951004/1‑94 (MQ=60)
         ACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACC                                                                                     >  SRR4302543.6537238/1‑94 (MQ=60)
          CCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCG                                                                                    <  SRR4302543.7310209/94‑1 (MQ=60)
           CGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGG                                                                                   >  SRR4302543.5509271/1‑94 (MQ=60)
           CGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGG                                                                                   >  SRR4302543.7701533/1‑94 (MQ=60)
           CGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGG                                                                                   >  SRR4302543.1225621/1‑94 (MQ=60)
           CGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGG                                                                                   <  SRR4302543.4300979/94‑1 (MQ=60)
            GTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGC                                                                                  >  SRR4302543.2242304/1‑94 (MQ=60)
            GTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGC                                                                                  >  SRR4302543.45787/1‑94 (MQ=60)
            GTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGC                                                                                  >  SRR4302543.2242096/1‑94 (MQ=60)
            GTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGC                                                                                  >  SRR4302543.5436235/1‑94 (MQ=60)
             TATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCT                                                                                 >  SRR4302543.6198805/1‑94 (MQ=60)
              ATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTG                                                                                <  SRR4302543.4771526/94‑1 (MQ=60)
              ATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTG                                                                                <  SRR4302543.5062758/94‑1 (MQ=60)
              ATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTG                                                                                >  SRR4302543.6223026/1‑94 (MQ=60)
               TTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGG                                                                               <  SRR4302543.6276714/94‑1 (MQ=60)
               TTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGG                                                                               >  SRR4302543.3977430/1‑94 (MQ=60)
               TTGCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGG                                                                               <  SRR4302543.2004361/94‑1 (MQ=60)
                TTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGT                                                                              <  SRR4302543.6497135/94‑1 (MQ=60)
                TTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGG                                                                              >  SRR4302543.1729540/1‑94 (MQ=60)
                 TCCCCGCGACCGCGGATTCAACCTGGTCACGCGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTG                                                                             <  SRR4302543.2357313/94‑1 (MQ=60)
                 TCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTG                                                                             <  SRR4302543.554948/94‑1 (MQ=60)
                 TCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTG                                                                             <  SRR4302543.5562530/94‑1 (MQ=60)
                 TCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTG                                                                             <  SRR4302543.3879662/94‑1 (MQ=60)
                  CCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGA                                                                            >  SRR4302543.1327994/1‑94 (MQ=60)
                  CCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGA                                                                            >  SRR4302543.2791157/1‑94 (MQ=60)
                  CCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGA                                                                            >  SRR4302543.6341467/1‑94 (MQ=60)
                  CCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTAA                                                                            >  SRR4302543.2780032/1‑94 (MQ=60)
                   CACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAG                                                                           <  SRR4302543.4033027/94‑1 (MQ=60)
                   CACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAG                                                                           >  SRR4302543.4224585/1‑94 (MQ=60)
                   CACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAG                                                                           <  SRR4302543.5547499/94‑1 (MQ=60)
                    ACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGT                                                                          <  SRR4302543.7149024/94‑1 (MQ=60)
                     CGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTC                                                                         <  SRR4302543.3713537/94‑1 (MQ=60)
                      GCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCG                                                                        <  SRR4302543.5551614/94‑1 (MQ=60)
                       CGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGG                                                                       <  SRR4302543.2271852/94‑1 (MQ=60)
                       CGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGG                                                                       <  SRR4302543.4830101/94‑1 (MQ=60)
                        GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGG                                                                      >  SRR4302543.3387732/1‑94 (MQ=60)
                        GACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGG                                                                      <  SRR4302543.5565131/94‑1 (MQ=60)
                          CCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCC                                                                    <  SRR4302543.7378373/94‑1 (MQ=60)
                             CGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTC                                                                 >  SRR4302543.1118612/1‑94 (MQ=60)
                             CGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTC                                                                 >  SRR4302543.5780788/1‑94 (MQ=60)
                              GGATTCAACCTGGTCACACGGACGCCATGTAGGCATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                <  SRR4302543.2955443/94‑1 (MQ=60)
                              GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                >  SRR4302543.7012708/1‑94 (MQ=60)
                              GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                <  SRR4302543.7009953/94‑1 (MQ=60)
                              GGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCT                                                                <  SRR4302543.1451613/94‑1 (MQ=60)
                               GATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTT                                                               >  SRR4302543.2513534/1‑94 (MQ=60)
                                ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                              >  SRR4302543.3827289/1‑94 (MQ=60)
                                ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                              >  SRR4302543.3667715/1‑94 (MQ=60)
                                ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                              >  SRR4302543.2982610/1‑94 (MQ=60)
                                ATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTC                                                              >  SRR4302543.5046126/1‑94 (MQ=60)
                                     ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                         <  SRR4302543.3629559/94‑1 (MQ=60)
                                     ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                         <  SRR4302543.238823/94‑1 (MQ=60)
                                     ACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCA                                                         <  SRR4302543.221402/94‑1 (MQ=60)
                                      CCTGGTCGCCCGGCCGCCATGTGGCCGTGTTCGGGGTTACGCGCAGGGAAGCGACTTGCTCGCCCGGCTGGTGAGTCGGGCCGTCTTCGCCCAG                                                        <  SRR4302543.6084636/94‑1 (MQ=38)
                                      CCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAG                                                        <  SRR4302543.7125793/94‑1 (MQ=60)
                                       CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGC                                                       >  SRR4302543.5524449/1‑94 (MQ=60)
                                       CTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGA                                                       >  SRR4302543.5496382/1‑94 (MQ=60)
                                         GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACC                                                     <  SRR4302543.1421693/94‑1 (MQ=60)
                                         GGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGGCGGGCCGTCCTCGCCCAGACC                                                     >  SRR4302543.7454802/1‑94 (MQ=60)
                                          GTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCG                                                    <  SRR4302543.7383979/94‑1 (MQ=60)
                                          GTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCG                                                    <  SRR4302543.3485695/94‑1 (MQ=60)
                                           TCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGA                                                   >  SRR4302543.5105855/1‑94 (MQ=60)
                                            CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGGCGTCTTCGCCCAGACAGAT                                                  >  SRR4302543.2914661/1‑94 (MQ=60)
                                            CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                  >  SRR4302543.3371417/1‑94 (MQ=60)
                                            CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                  <  SRR4302543.6769799/94‑1 (MQ=60)
                                            CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAT                                                  <  SRR4302543.4740721/94‑1 (MQ=60)
                                            CACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGAG                                                  >  SRR4302543.3646971/1‑94 (MQ=60)
                                             CCACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 <  SRR4302543.2230263/94‑1 (MQ=60)
                                             ACACGGTCGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 <  SRR4302543.4895769/94‑1 (MQ=60)
                                             ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 <  SRR4302543.488648/94‑1 (MQ=60)
                                             ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 >  SRR4302543.1580849/1‑94 (MQ=60)
                                             ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 >  SRR4302543.6046153/1‑94 (MQ=60)
                                             ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 <  SRR4302543.5012664/94‑1 (MQ=60)
                                             ACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATG                                                 >  SRR4302543.7337555/1‑94 (MQ=60)
                                              CACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGG                                                <  SRR4302543.1188526/94‑1 (MQ=60)
                                              CACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGG                                                <  SRR4302543.256043/94‑1 (MQ=60)
                                               ACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGA                                               <  SRR4302543.2611786/94‑1 (MQ=60)
                                                CGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAG                                              <  SRR4302543.2529781/94‑1 (MQ=60)
                                                CGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCGCCCAGACCGATGGAG                                              >  SRR4302543.4532275/1‑94 (MQ=60)
                                                  GACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                            <  SRR4302543.3600317/94‑1 (MQ=60)
                                                  GACGCCATGTAGACATGGTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTC                                            <  SRR4302543.457731/94‑1 (MQ=60)
                                                   ACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCG                                           <  SRR4302543.5653239/94‑1 (MQ=60)
                                                   ACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCG                                           <  SRR4302543.4680065/94‑1 (MQ=60)
                                                     GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGTCCAGACCGATGGAGTCGTG                                         >  SRR4302543.3664191/1‑94 (MQ=60)
                                                     GCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTG                                         <  SRR4302543.2544320/94‑1 (MQ=60)
                                                      CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                        <  SRR4302543.2879046/94‑1 (MQ=60)
                                                      CCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGG                                        <  SRR4302543.5874674/94‑1 (MQ=60)
                                                       CATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGG                                       <  SRR4302543.2569849/94‑1 (MQ=60)
                                                        ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                      <  SRR4302543.6422212/94‑1 (MQ=60)
                                                        ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                      <  SRR4302543.3816155/94‑1 (MQ=60)
                                                        ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGT                                      >  SRR4302543.4923276/1‑94 (MQ=60)
                                                        ATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGG                                      >  SRR4302543.4924457/1‑94 (MQ=60)
                                                         TGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTG                                     >  SRR4302543.3289898/1‑94 (MQ=60)
                                                         TGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTG                                     <  SRR4302543.7480789/94‑1 (MQ=60)
                                                          GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                    >  SRR4302543.5277186/1‑94 (MQ=60)
                                                          GTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGT                                    >  SRR4302543.5277180/1‑94 (MQ=60)
                                                           TAGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTA                                   <  SRR4302543.6069697/94‑1 (MQ=60)
                                                            AGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                  >  SRR4302543.6868888/1‑94 (MQ=60)
                                                            AGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAA                                  >  SRR4302543.1027954/1‑94 (MQ=60)
                                                            AGACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGCAGTGGGTGTAA                                  >  SRR4302543.1027953/1‑94 (MQ=60)
                                                             GACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGGGTAAA                                 >  SRR4302543.6589640/1‑94 (MQ=60)
                                                              ACATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAAC                                <  SRR4302543.90609/94‑1 (MQ=60)
                                                               CATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                               >  SRR4302543.4254961/1‑94 (MQ=60)
                                                               CATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACC                               <  SRR4302543.7842319/94‑1 (MQ=60)
                                                                ATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                              >  SRR4302543.1559981/1‑94 (MQ=60)
                                                                ATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                              >  SRR4302543.2715087/1‑94 (MQ=60)
                                                                ATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                              >  SRR4302543.2416402/1‑94 (MQ=60)
                                                                ATGTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCA                              >  SRR4302543.1086895/1‑94 (MQ=60)
                                                                  GTTCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCTGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATC                            <  SRR4302543.3613048/94‑1 (MQ=60)
                                                                    TCGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCAC                          <  SRR4302543.2650109/94‑1 (MQ=60)
                                                                     CGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGTGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                         <  SRR4302543.4466103/94‑1 (MQ=60)
                                                                     CGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                         <  SRR4302543.6808876/94‑1 (MQ=60)
                                                                     CGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                         <  SRR4302543.4466104/94‑1 (MQ=60)
                                                                     CGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                         <  SRR4302543.2292796/94‑1 (MQ=60)
                                                                     CGGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACC                         >  SRR4302543.3421423/1‑94 (MQ=60)
                                                                      GGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                        >  SRR4302543.6807462/1‑94 (MQ=60)
                                                                      GGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                        <  SRR4302543.3043987/94‑1 (MQ=60)
                                                                      GGGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCT                        <  SRR4302543.3248795/94‑1 (MQ=60)
                                                                       GGGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTG                       <  SRR4302543.1926803/94‑1 (MQ=60)
                                                                        GGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACatgc                      >  SRR4302543.2019323/1‑90 (MQ=60)
                                                                        GGTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGA                      <  SRR4302543.1274309/94‑1 (MQ=60)
                                                                         GTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                     <  SRR4302543.6327536/94‑1 (MQ=60)
                                                                         GTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                     <  SRR4302543.1842034/94‑1 (MQ=60)
                                                                         GTTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGAC                     <  SRR4302543.7844835/94‑1 (MQ=60)
                                                                          GTACGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACG                    <  SRR4302543.3318306/94‑1 (MQ=60)
                                                                             CGCGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTG                 >  SRR4302543.7054636/1‑94 (MQ=60)
                                                                               CGCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTT               >  SRR4302543.5557031/1‑94 (MQ=60)
                                                                                GCAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTT              <  SRR4302543.2074471/94‑1 (MQ=60)
                                                                                 CAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC             >  SRR4302543.1568051/1‑94 (MQ=60)
                                                                                 CAGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTC             <  SRR4302543.4900390/94‑1 (MQ=60)
                                                                                 CAGAGAAGCGACTTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGACGTGGGTGTAAACCATCACCTGACGCTGTTTC             >  SRR4302543.658439/1‑94 (MQ=60)
                                                                                  AGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA            >  SRR4302543.4377082/1‑94 (MQ=60)
                                                                                  AGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA            >  SRR4302543.4402140/1‑94 (MQ=60)
                                                                                  AGAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCA            >  SRR4302543.4807776/1‑94 (MQ=60)
                                                                                   GAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCAT           >  SRR4302543.2588656/1‑94 (MQ=60)
                                                                                   GAGAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCAT           <  SRR4302543.5176485/94‑1 (MQ=60)
                                                                                   GAGAAGCGACTTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCAT           >  SRR4302543.1318417/1‑94 (MQ=60)
                                                                                     GAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA         <  SRR4302543.1189684/94‑1 (MQ=60)
                                                                                     GAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA         <  SRR4302543.1061799/94‑1 (MQ=60)
                                                                                     GAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA         <  SRR4302543.2502029/94‑1 (MQ=60)
                                                                                     GAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA         >  SRR4302543.4282890/1‑94 (MQ=60)
                                                                                     GAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA         <  SRR4302543.4449829/94‑1 (MQ=60)
                                                                                     GAAGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGGCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCA         >  SRR4302543.2599411/1‑94 (MQ=60)
                                                                                      AAGCGACTTGCTCAACCGGCTGGGGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACGTGACGATGTTTAATCAC        >  SRR4302543.3895531/1‑94 (MQ=60)
                                                                                       AGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC       >  SRR4302543.140781/1‑94 (MQ=60)
                                                                                       AGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC       <  SRR4302543.3322866/94‑1 (MQ=60)
                                                                                       AGCGACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGC       <  SRR4302543.5615538/94‑1 (MQ=60)
                                                                                          GACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA    >  SRR4302543.2354373/1‑94 (MQ=60)
                                                                                          GACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA    <  SRR4302543.3122226/94‑1 (MQ=60)
                                                                                          GACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCA    >  SRR4302543.4336518/1‑94 (MQ=60)
                                                                                           ACTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAG   <  SRR4302543.255917/94‑1 (MQ=60)
                                                                                            CTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC  >  SRR4302543.7326468/1‑94 (MQ=60)
                                                                                            CTTGCTCAACCGGCTGGTGAGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC  >  SRR4302543.4278751/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCAGAGAAGCGACCTGCTCAACCGGCTGGTGAGTCGGCCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTAAACCATCACCTGACGCTGTTTCATCAGCGCAGC  >  NC_000913/3078128‑3078313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑