Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,107,469 +C intergenic (‑296/‑101) b2965 ← / → yqgA ornithine decarboxylase isozyme/putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,4691.C98.9% 1097.7 / 2.7 355intergenic (‑296/‑101)b2965/yqgAornithine decarboxylase isozyme/putative transport protein
Reads supporting (aligned to +/- strand):  ref base . (1/3);  new base C (152/199);  total (153/202)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 392 total aligned reads displayed.

GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC  >  NC_000913/3107377‑3107555
                                                                                              |                                                                                      
gAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑t                                                                                         <  1:1427458/93‑1 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGACAATTCGCGCACTCATGGCACCtt‑tc                                                                                        >  1:6413677/1‑92 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tc                                                                                        <  1:2788073/93‑2 (MQ=255)
 aaaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tc                                                                                        <  2:3358551/93‑2 (MQ=255)
  aaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcg                                                                                       <  2:5565887/93‑3 (MQ=255)
  aaCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcg                                                                                       <  2:1070566/93‑3 (MQ=255)
   aCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcgc                                                                                      <  1:3323015/93‑4 (MQ=255)
   aCGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCtt‑tcgc                                                                                      >  1:6719196/1‑90 (MQ=255)
      gCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTaa                                                                                   >  1:4342828/1‑93 (MQ=255)
       cGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTaaa                                                                                  <  2:1208962/93‑1 (MQ=255)
       cGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTaaa                                                                                  <  2:1350125/93‑1 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAg                                                                                 >  2:4740668/1‑93 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAg                                                                                 >  2:2821913/1‑93 (MQ=255)
        gATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAg                                                                                 <  1:3473582/93‑1 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGc                                                                                <  2:3691264/93‑1 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGc                                                                                <  1:4551085/93‑1 (MQ=255)
         aTAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGc                                                                                >  2:2404737/1‑93 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCg                                                                               >  2:6513790/1‑93 (MQ=255)
          tAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCg                                                                               >  1:4696779/1‑93 (MQ=255)
            aGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTg                                                                             <  1:1645339/93‑1 (MQ=255)
              ggagagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCa                                                                           <  2:1743189/92‑1 (MQ=255)
                agagaATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAg                                                                         >  1:6004445/1‑93 (MQ=255)
                    aaTGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAg                                                                     >  2:6126701/1‑93 (MQ=255)
                     aTGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAgg                                                                    >  1:3849827/1‑93 (MQ=255)
                      tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGa                                                                   >  2:3084524/1‑93 (MQ=255)
                      tGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGa                                                                   <  1:1105846/93‑1 (MQ=255)
                         aaGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACtt                                                                <  1:5313066/93‑1 (MQ=255)
                          aGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACttt                                                               <  1:4976618/93‑1 (MQ=255)
                          aGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACttt                                                               <  2:4638180/93‑1 (MQ=255)
                          aGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACttt                                                               <  1:6515412/93‑1 (MQ=255)
                           ggAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTg                                                              >  2:2524941/1‑93 (MQ=255)
                            gagaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGc                                                             <  2:814412/93‑1 (MQ=255)
                              gaTGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGa                                                           >  1:2222917/1‑93 (MQ=255)
                                tGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaac                                                         >  1:2711355/1‑93 (MQ=255)
                                 ggCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaaca                                                        >  1:2122993/1‑93 (MQ=255)
                                 ggCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaaca                                                        <  2:7481226/93‑1 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    >  1:6294323/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    >  1:7598830/1‑93 (MQ=255)
                                     tGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGaacaaaca                                                    >  2:6747849/1‑93 (MQ=255)
                                       cATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAg                                                  <  1:3681049/93‑1 (MQ=255)
                                         tCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGcc                                                <  1:2023877/93‑1 (MQ=255)
                                         tCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGcc                                                >  2:5948830/1‑93 (MQ=255)
                                          cGGACAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCa                                               <  2:3930607/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              <  1:3533177/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              <  1:2584080/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              <  1:7085252/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              <  2:7178292/93‑1 (MQ=255)
                                           gggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAt                                              <  2:7773897/93‑1 (MQ=255)
                                            ggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATc                                             <  2:5363363/93‑1 (MQ=255)
                                            ggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATc                                             <  1:58035/93‑1 (MQ=255)
                                            ggCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATc                                             <  1:4116777/93‑1 (MQ=255)
                                              gaaaCTCCGTACAGAGAGGCAAAAATACGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCAAGAACTTTGCGAACAAACAAGCCATcaa                                           <  1:7034508/92‑3 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  2:4206039/93‑1 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  1:515261/93‑1 (MQ=255)
                                               aaaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGAc                                          <  2:2259862/93‑1 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         <  2:6022000/93‑1 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         >  1:6931589/1‑93 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         >  1:4897452/1‑93 (MQ=255)
                                                aaCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACg                                         <  1:4681365/93‑1 (MQ=255)
                                                  cTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGc                                       >  1:1176416/1‑93 (MQ=255)
                                                   tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCa                                      >  2:4584309/1‑93 (MQ=255)
                                                   tCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCa                                      >  1:1589123/1‑93 (MQ=255)
                                                      gTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCa                                   >  1:990898/1‑93 (MQ=255)
                                                      gTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCa                                   >  1:6829524/1‑93 (MQ=255)
                                                       tACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaa                                  >  1:1141705/1‑93 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGCCTTTGCGAACAACCAAGCCATCGACGGCACGaaa                                 >  2:6782477/1‑93 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 <  2:3484938/93‑1 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCaaa                                 <  1:4298097/93‑1 (MQ=255)
                                                        aCAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACCGCACCaaa                                 >  1:1324311/1‑93 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                >  2:1495264/1‑93 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                <  1:3024275/93‑1 (MQ=255)
                                                         cAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAc                                <  2:627360/93‑1 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              >  2:7627615/1‑93 (MQ=255)
                                                           gagagGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACgg                              >  2:5688429/1‑93 (MQ=255)
                                                            agagGAGAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGt                             <  1:3382609/93‑1 (MQ=255)
                                                               ggagAAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCat                          <  2:1992144/93‑1 (MQ=255)
                                                               ggagAAAATGCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCat                          <  2:1835227/93‑1 (MQ=255)
                                                                gagaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCata                         <  1:5660534/93‑1 (MQ=255)
                                                                 agaAAATTCGCGCAATCATGGCCCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataa                        <  2:6661344/93‑1 (MQ=255)
                                                                  gaAAATTCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCataat                       <  2:4514439/93‑1 (MQ=255)
                                                                       ttCTCGCAATCATGGCTCTTT‑TCGTTAATGCGCGCAAGCCAGGACTTTTCGAACAAACAAGCCATTGACGGCACCAAACGGTCATAATATGaa                  >  2:7273296/1‑93 (MQ=255)
                                                                       ttCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaa                  <  1:6161528/93‑1 (MQ=255)
                                                                       ttCGCGCAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGaa                  <  2:5793466/93‑1 (MQ=255)
                                                                          gcgcAATCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAAt               >  2:5297794/1‑93 (MQ=255)
                                                                             cAATCATGGCGCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCaa            <  2:1412770/93‑1 (MQ=255)
                                                                               aTCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAc          <  2:6461952/93‑1 (MQ=255)
                                                                                tCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa         <  2:3835279/93‑1 (MQ=255)
                                                                                tCATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACa         >  1:242553/1‑93 (MQ=255)
                                                                                 cATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACaa        >  2:4401319/1‑93 (MQ=255)
                                                                                 cATGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACaa        <  1:3244883/93‑1 (MQ=255)
                                                                                  agggCACCTTCC‑GCTAAAGCGTGCAAGGCAGTACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAt       <  2:4023102/91‑1 (MQ=255)
                                                                                  aTGGCCCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAt       <  2:4974459/93‑1 (MQ=255)
                                                                                  aTGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAt       >  1:7267401/1‑93 (MQ=255)
                                                                                  aTGGCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAt       <  1:1835501/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    <  1:7769007/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    <  1:7471291/93‑1 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    >  2:5168669/1‑93 (MQ=255)
                                                                                     gCACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACa    >  1:1902185/1‑93 (MQ=255)
                                                                                      cACCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAg   <  2:1592942/93‑1 (MQ=255)
                                                                                       aCCTT‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGc  >  1:5920514/1‑93 (MQ=255)
                                                                                              |                                                                                      
GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC  >  NC_000913/3107377‑3107555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 223 total aligned reads displayed.
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562
                                                                                                |                                                                                              
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑T                                                                                                >  SRR4302543.3638651/1‑94 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAATCTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑T‑C                                                                                              >  SRR4302543.6474083/1‑94 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGACAATTCGCGCACTCATGGCACC‑T‑T‑T‑C                                                                                              >  SRR4302543.6474084/1‑94 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑T‑C                                                                                              <  SRR4302543.1440372/94‑1 (MQ=60)
 GAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑T‑C                                                                                              >  SRR4302543.7816361/1‑94 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑Tcg                                                                                              <  SRR4302543.2813519/94‑3 (MQ=60)
  AAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑Tcg                                                                                              >  SRR4302543.352700/1‑92 (MQ=60)
   AACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑Tcgc                                                                                             >  SRR4302543.6782703/1‑91 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCT                                                                                            >  SRR4302543.4911589/1‑94 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCT                                                                                            <  SRR4302543.3352991/94‑1 (MQ=60)
    ACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCT                                                                                            <  SRR4302543.1175202/94‑1 (MQ=60)
     CGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTA                                                                                           >  SRR4302543.1334418/1‑94 (MQ=60)
     CGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTA                                                                                           >  SRR4302543.7894347/1‑94 (MQ=60)
      GGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAA                                                                                          >  SRR4302543.4382218/1‑94 (MQ=60)
      GGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAA                                                                                          >  SRR4302543.4382216/1‑94 (MQ=60)
       GCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAA                                                                                         <  SRR4302543.6276265/94‑1 (MQ=60)
       GCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAA                                                                                         >  SRR4302543.6789386/1‑94 (MQ=60)
        CGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAG                                                                                        <  SRR4302543.2274103/94‑1 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGC                                                                                       >  SRR4302543.1474980/1‑94 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGC                                                                                       <  SRR4302543.5848208/94‑1 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGC                                                                                       <  SRR4302543.1780071/94‑1 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGC                                                                                       <  SRR4302543.3504697/94‑1 (MQ=60)
         GATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGC                                                                                       >  SRR4302543.7166587/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCG                                                                                      >  SRR4302543.4739671/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCG                                                                                      >  SRR4302543.6249914/1‑94 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCG                                                                                      <  SRR4302543.3663252/94‑1 (MQ=60)
          ATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCG                                                                                      <  SRR4302543.4592533/94‑1 (MQ=60)
           TAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGT                                                                                     <  SRR4302543.3162122/94‑1 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTG                                                                                    <  SRR4302543.2812778/94‑1 (MQ=60)
            AAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTG                                                                                    <  SRR4302543.1210662/94‑1 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGC                                                                                   >  SRR4302543.6765308/1‑94 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGC                                                                                   <  SRR4302543.4001903/94‑1 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGC                                                                                   <  SRR4302543.1660040/94‑1 (MQ=60)
             AGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGC                                                                                   <  SRR4302543.1660041/94‑1 (MQ=60)
              GCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCA                                                                                  >  SRR4302543.2044578/1‑94 (MQ=60)
              GCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCA                                                                                  <  SRR4302543.7461950/94‑1 (MQ=60)
               CGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAA                                                                                 >  SRR4302543.6401417/1‑94 (MQ=60)
               CGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAA                                                                                 >  SRR4302543.2721493/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAG                                                                                >  SRR4302543.3548402/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAG                                                                                >  SRR4302543.6059671/1‑94 (MQ=60)
                GAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAG                                                                                <  SRR4302543.4956842/94‑1 (MQ=60)
                 AGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGC                                                                               >  SRR4302543.1513202/1‑94 (MQ=60)
                 AGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGC                                                                               >  SRR4302543.4722118/1‑94 (MQ=60)
                  GAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCC                                                                              >  SRR4302543.205126/1‑94 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCA                                                                             <  SRR4302543.6964831/94‑1 (MQ=60)
                   AGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGCCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCA                                                                             >  SRR4302543.3467297/1‑94 (MQ=60)
                    GAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑TCC‑GCTAAAGCGTGCAAGCCAG                                                                            <  SRR4302543.7475531/94‑1 (MQ=60)
                    GAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAG                                                                            <  SRR4302543.5784576/94‑1 (MQ=60)
                    GAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAG                                                                            <  SRR4302543.6330217/94‑1 (MQ=60)
                    GAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAG                                                                            <  SRR4302543.4567879/94‑1 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302543.5299920/94‑1 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGG                                                                           <  SRR4302543.4346226/94‑1 (MQ=60)
                     AATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGG                                                                           >  SRR4302543.3883830/1‑94 (MQ=60)
                      ATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGA                                                                          >  SRR4302543.6197168/1‑94 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGAC                                                                         <  SRR4302543.1115819/94‑1 (MQ=60)
                       TGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGAC                                                                         <  SRR4302543.2802926/94‑1 (MQ=60)
                        GTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACT                                                                        >  SRR4302543.1816069/1‑94 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      >  SRR4302543.3270253/1‑94 (MQ=60)
                          AAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTT                                                                      <  SRR4302543.5361063/94‑1 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     <  SRR4302543.5021918/94‑1 (MQ=60)
                           AGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTG                                                                     <  SRR4302543.6576778/94‑1 (MQ=60)
                            GGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGC                                                                    >  SRR4302543.1875292/1‑94 (MQ=60)
                             GAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCG                                                                   <  SRR4302543.217254/94‑1 (MQ=60)
                              AGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGA                                                                  >  SRR4302543.2242981/1‑94 (MQ=60)
                              AGATGGCATGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTACGA                                                                  >  SRR4302543.7038110/1‑94 (MQ=60)
                                ATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAAC                                                                >  SRR4302543.2735985/1‑94 (MQ=60)
                                 TGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACA                                                               >  SRR4302543.2142004/1‑94 (MQ=60)
                                     GTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACA                                                           <  SRR4302543.7179144/94‑1 (MQ=60)
                                     GTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACA                                                           >  SRR4302543.6353576/1‑94 (MQ=60)
                                      TGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAA                                                          >  SRR4302543.1143907/1‑94 (MQ=60)
                                       GCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAG                                                         <  SRR4302543.553504/94‑1 (MQ=60)
                                        CATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGC                                                        <  SRR4302543.3713395/94‑1 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       >  SRR4302543.3287554/1‑94 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       >  SRR4302543.3287553/1‑94 (MQ=60)
                                         ATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCC                                                       >  SRR4302543.7011625/1‑94 (MQ=60)
                                           CGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAT                                                     <  SRR4302543.6493748/94‑1 (MQ=60)
                                           CGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCAT                                                     <  SRR4302543.59227/94‑1 (MQ=60)
                                           CGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGACAGGACTTTGCGAACAAACAAGCCAT                                                     >  SRR4302543.3288713/1‑94 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    <  SRR4302543.7154062/94‑1 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    >  SRR4302543.2003956/1‑94 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    <  SRR4302543.3564623/94‑1 (MQ=60)
                                            GGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC                                                    <  SRR4302543.2607593/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   <  SRR4302543.5121/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   <  SRR4302543.6975281/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   <  SRR4302543.58547/94‑1 (MQ=60)
                                             GGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCG                                                   <  SRR4302543.4153859/94‑1 (MQ=60)
                                                AAACTCCGTACAGTGAGGAGAAAATTCGCGCATTCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                >  SRR4302543.6056514/1‑94 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                >  SRR4302543.4942097/1‑94 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                <  SRR4302543.520050/94‑1 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                >  SRR4302543.4155218/1‑94 (MQ=60)
                                                AAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACG                                                >  SRR4302543.6996790/1‑94 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               >  SRR4302543.4919791/1‑94 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               <  SRR4302543.4724051/94‑1 (MQ=60)
                                                 AACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGG                                               >  SRR4302543.586368/1‑94 (MQ=60)
                                                  ACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGC                                              >  SRR4302543.1187069/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             <  SRR4302543.4347903/94‑1 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             >  SRR4302543.6752656/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             >  SRR4302543.1603308/1‑94 (MQ=60)
                                                   CTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCA                                             >  SRR4302543.3411641/1‑94 (MQ=60)
                                                    TCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCAC                                            <  SRR4302543.1917177/94‑1 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAGGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          >  SRR4302543.999644/1‑94 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          >  SRR4302543.7369509/1‑94 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          >  SRR4302543.6893997/1‑94 (MQ=60)
                                                      CGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCA                                          <  SRR4302543.1981907/94‑1 (MQ=60)
                                                       GTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAA                                         >  SRR4302543.1152106/1‑94 (MQ=60)
                                                       GTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAA                                         >  SRR4302543.21833/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACCTT‑T‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCCAA                                        >  SRR4302543.7062920/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAA                                        >  SRR4302543.1182011/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAA                                        >  SRR4302543.1336069/1‑94 (MQ=60)
                                                        TACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACCGCACCAAA                                        >  SRR4302543.1336068/1‑94 (MQ=60)
                                                         ACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302543.4337155/94‑1 (MQ=60)
                                                         ACAGAGAAGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAGGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAAC                                       <  SRR4302543.1983553/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302543.2525794/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302543.2175854/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302543.2722982/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302543.4110747/94‑1 (MQ=60)
                                                          CAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACG                                      <  SRR4302543.55208/94‑1 (MQ=60)
                                                           AGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGG                                     >  SRR4302543.2940517/1‑94 (MQ=60)
                                                            GAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGT                                    <  SRR4302543.2823319/94‑1 (MQ=60)
                                                            GAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGT                                    <  SRR4302543.5015495/94‑1 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   >  SRR4302543.6991777/1‑94 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   >  SRR4302543.5007676/1‑94 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   <  SRR4302543.3412947/94‑1 (MQ=60)
                                                             AGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTC                                   >  SRR4302543.1656632/1‑94 (MQ=60)
                                                               AGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCAT                                 >  SRR4302543.7127459/1‑94 (MQ=60)
                                                                GGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATA                                <  SRR4302543.7654552/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCCCC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATC‑ACGGCACCAAACGGTCATAAT                              <  SRR4302543.6767069/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               <  SRR4302543.5712164/94‑1 (MQ=60)
                                                                 GAGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAA                               >  SRR4302543.7829363/1‑94 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              <  SRR4302543.843793/94‑1 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              >  SRR4302543.7141555/1‑94 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              <  SRR4302543.3061731/94‑1 (MQ=60)
                                                                  AGAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGCGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAAT                              <  SRR4302543.4094338/94‑1 (MQ=60)
                                                                   GAAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATA                             <  SRR4302543.5959165/94‑1 (MQ=60)
                                                                   GAAAATCCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATA                             <  SRR4302543.3835181/94‑1 (MQ=60)
                                                                    AAAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAA                            <  SRR4302543.1397118/94‑1 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302543.4727878/94‑1 (MQ=60)
                                                                     AAATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAG                           <  SRR4302543.1304202/94‑1 (MQ=60)
                                                                      AATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGA                          <  SRR4302543.301308/94‑1 (MQ=60)
                                                                      AATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGA                          <  SRR4302543.1862549/94‑1 (MQ=60)
                                                                      AATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGA                          >  SRR4302543.7553481/1‑94 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         <  SRR4302543.3469606/94‑1 (MQ=60)
                                                                       ATTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAA                         <  SRR4302543.2486595/94‑1 (MQ=60)
                                                                        TTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAA                        >  SRR4302543.6533343/1‑94 (MQ=60)
                                                                        TTCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAA                        <  SRR4302543.6219842/94‑1 (MQ=60)
                                                                         TCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAA                       <  SRR4302543.6029397/94‑1 (MQ=60)
                                                                         TCGCGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAA                       <  SRR4302543.1155792/94‑1 (MQ=60)
                                                                            CGCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCA                    <  SRR4302543.3601592/94‑1 (MQ=60)
                                                                             GCAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAA                   >  SRR4302543.4728554/1‑94 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  >  SRR4302543.4674112/1‑94 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  <  SRR4302543.7739971/94‑1 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  >  SRR4302543.4232589/1‑94 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  >  SRR4302543.1364762/1‑94 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  <  SRR4302543.5327607/94‑1 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  <  SRR4302543.3373562/94‑1 (MQ=60)
                                                                              CAATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAA                  <  SRR4302543.5327608/94‑1 (MQ=60)
                                                                               AATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 >  SRR4302543.6817865/1‑94 (MQ=60)
                                                                               AATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 >  SRR4302543.5062801/1‑94 (MQ=60)
                                                                               AATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAAC                 <  SRR4302543.3842844/94‑1 (MQ=60)
                                                                                ATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302543.6362313/94‑1 (MQ=60)
                                                                                ATCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                >  SRR4302543.244656/1‑94 (MQ=60)
                                                                                ATCATGGCACC‑T‑T‑TCGATAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACA                <  SRR4302543.7113102/94‑1 (MQ=60)
                                                                                 TCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               <  SRR4302543.4727405/94‑1 (MQ=60)
                                                                                 TCATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAA               >  SRR4302543.6127675/1‑94 (MQ=60)
                                                                                  CATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGCCTTTGCGAACAACCAAACCAAAGACGGCACCTAACGGTCATACTAAGAAAAACAACCAAT              >  SRR4302543.7118166/1‑94 (MQ=38)
                                                                                  CATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAT              >  SRR4302543.763314/1‑94 (MQ=60)
                                                                                  CATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAT              >  SRR4302543.7337612/1‑94 (MQ=60)
                                                                                  CATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAAT              <  SRR4302543.3274227/94‑1 (MQ=60)
                                                                                  CATGACACC‑T‑T‑TCGCTAAAGCGTGCAACCCAGGACTTTGCGAACAAACAAGCCATCGcacacaccaaccggtcatcataagaacatccacagaa              >  SRR4302543.7118167/1‑57 (MQ=51)
                                                                                   ATGGCACC‑TGT‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATA             <  SRR4302543.3502555/94‑1 (MQ=60)
                                                                                   ATGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATA             <  SRR4302543.1852028/94‑1 (MQ=60)
                                                                                    TGGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATAC            <  SRR4302543.4543339/94‑1 (MQ=60)
                                                                                     GGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           <  SRR4302543.1128218/94‑1 (MQ=60)
                                                                                     GGCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACA           >  SRR4302543.1919302/1‑94 (MQ=60)
                                                                                      GCCCC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302543.5811414/94‑1 (MQ=60)
                                                                                      GCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302543.7842304/94‑1 (MQ=60)
                                                                                      GCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302543.5608703/94‑1 (MQ=60)
                                                                                      GCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302543.2118211/94‑1 (MQ=60)
                                                                                      GCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302543.2444540/94‑1 (MQ=60)
                                                                                      GCACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAG          <  SRR4302543.7542840/94‑1 (MQ=60)
                                                                                       CACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         >  SRR4302543.5974598/1‑94 (MQ=60)
                                                                                       CACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         >  SRR4302543.163927/1‑94 (MQ=60)
                                                                                       CACC‑T‑T‑TCACTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGC         <  SRR4302543.2506454/94‑1 (MQ=60)
                                                                                        ACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCT        >  SRR4302543.6182708/1‑94 (MQ=60)
                                                                                        ACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCT        >  SRR4302543.1469060/1‑94 (MQ=60)
                                                                                        ACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCT        >  SRR4302543.356212/1‑94 (MQ=60)
                                                                                        ACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCT        <  SRR4302543.2736247/94‑1 (MQ=60)
                                                                                        ACC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCT        >  SRR4302543.6182707/1‑94 (MQ=60)
                                                                                         CC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       >  SRR4302543.1447048/1‑94 (MQ=60)
                                                                                         CC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       >  SRR4302543.2303598/1‑94 (MQ=60)
                                                                                         CC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       <  SRR4302543.7228959/94‑1 (MQ=60)
                                                                                         CC‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTA       >  SRR4302543.926798/1‑94 (MQ=60)
                                                                                          C‑T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAA      >  SRR4302543.7171430/1‑94 (MQ=60)
                                                                                            T‑T‑TCGCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAAC     <  SRR4302543.3442211/94‑1 (MQ=60)
                                                                                              T‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAG   <  SRR4302543.6831068/94‑1 (MQ=60)
                                                                                              T‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAG   <  SRR4302543.3588574/94‑1 (MQ=60)
                                                                                              T‑C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAG   <  SRR4302543.3651635/94‑1 (MQ=60)
                                                                                                C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302543.5945156/1‑94 (MQ=60)
                                                                                                C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  SRR4302543.5075240/1‑94 (MQ=60)
                                                                                                C‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  <  SRR4302543.3968092/94‑1 (MQ=60)
                                                                                                |                                                                                              
CGAAACGGCGATAAGCGAGAGAATGTAAGGAGATGGCGTGCATCGGGCAAACTCCGTACAGAGAGGAGAAAATTCGCGCAATCATGGCACC‑T‑T‑T‑GCTAAAGCGTGCAAGCCAGGACTTTGCGAACAAACAAGCCATCGACGGCACCAAACGGTCATAATAAGAAAATCAAACAATACAGCTAACAGG  >  NC_000913/3107376‑3107562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑