Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,125,359 T→C K226K (AAA→AAG)  glcD ← glycolate oxidase subunit D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,125,3590TC99.6% 878.3 / NA 282K226K (AAA→AAG) glcDglycolate oxidase subunit D
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (150/129);  total (153/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448
                                                                                          |                                                                                         
 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                                                                                        >  2:5661728/1‑93 (MQ=255)
  cGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTa                                                                                       <  2:3806679/93‑1 (MQ=255)
  cGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTa                                                                                       <  1:2516404/93‑1 (MQ=255)
  cGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTa                                                                                       <  1:6711256/93‑1 (MQ=255)
   gatgatGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAc                                                                                      >  2:4348264/1‑93 (MQ=255)
   gatgatGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAc                                                                                      >  1:2874221/1‑93 (MQ=255)
    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     >  1:6811585/1‑93 (MQ=255)
    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     >  1:2707394/1‑93 (MQ=255)
    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     >  2:2645631/1‑93 (MQ=255)
    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTAACGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     <  2:4092478/93‑1 (MQ=255)
    atgataTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCATGGGCAGCTTCGGCAGCAGCTTTAcc                                                                                     >  1:6834637/1‑93 (MQ=255)
     tgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCg                                                                                    >  1:1714615/1‑93 (MQ=255)
      gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGt                                                                                   >  2:5106523/1‑93 (MQ=255)
      gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGt                                                                                   <  1:2586788/93‑1 (MQ=255)
        tgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCa                                                                                 >  1:1513089/1‑93 (MQ=255)
         gTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCAc                                                                                <  2:3577321/93‑1 (MQ=255)
          tCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACt                                                                               >  1:4901439/1‑93 (MQ=255)
            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCCGCTTTACCGTCACttt                                                                             >  2:6675353/1‑92 (MQ=255)
            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             >  1:7366652/1‑93 (MQ=255)
            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  2:2919889/93‑1 (MQ=255)
               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          >  1:6197836/1‑93 (MQ=255)
               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          <  1:7422297/93‑1 (MQ=255)
                 ccGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtgg                                                                        >  2:1040250/1‑93 (MQ=255)
                  cGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtggt                                                                       <  2:3152181/93‑1 (MQ=255)
                   gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTc                                                                      >  1:7869976/1‑93 (MQ=255)
                   gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTc                                                                      >  1:6327971/1‑93 (MQ=255)
                    cAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCa                                                                     <  1:5086489/93‑1 (MQ=255)
                     aaGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAc                                                                    <  2:895118/93‑1 (MQ=255)
                       gcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGc                                                                  >  2:2026191/3‑93 (MQ=255)
                       gcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGc                                                                  <  1:5990271/91‑1 (MQ=255)
                         ccGGCTTTTTCTACCGAGTCAACGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTCCCGTCACTTCGCTGGTTGCGCCg                                                                >  1:4064730/1‑93 (MQ=255)
                         ccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCg                                                                >  2:2447113/1‑93 (MQ=255)
                         ccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCg                                                                <  1:2042856/93‑1 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               <  1:466989/93‑1 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               <  2:976906/93‑1 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               <  1:6985479/93‑1 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               <  1:193626/93‑1 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               <  2:5224493/93‑1 (MQ=255)
                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               >  2:4989798/1‑93 (MQ=255)
                           ggCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAg                                                              >  1:933334/1‑93 (MQ=255)
                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  2:1960007/1‑93 (MQ=255)
                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             <  2:327262/93‑1 (MQ=255)
                             cTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCa                                                            <  2:3990317/93‑1 (MQ=255)
                              tttttCTACCGAGTCAAAGCTGGCTAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAt                                                           <  1:84592/93‑1 (MQ=255)
                                tttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAc                                                         >  1:3992257/1‑93 (MQ=255)
                                     aCCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTcc                                                    >  2:1526670/1‑93 (MQ=255)
                                     aCCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTcc                                                    >  1:4820515/1‑93 (MQ=255)
                                      ccGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGATTTACCGTCACTTCGGTGGTCACGCCGAGCATACCGTCCg                                                   >  1:5763733/1‑93 (MQ=255)
                                       cGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGa                                                  >  1:1237180/1‑93 (MQ=255)
                                         aGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATc                                                <  2:2351155/93‑1 (MQ=255)
                                          gTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATcc                                               >  1:1800021/1‑93 (MQ=255)
                                           tCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCg                                              >  2:2407717/1‑93 (MQ=255)
                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            <  2:6072339/93‑1 (MQ=255)
                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            >  1:4205826/1‑93 (MQ=255)
                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            >  2:227010/1‑93 (MQ=255)
                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           >  1:783741/1‑93 (MQ=255)
                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           >  1:6626679/1‑93 (MQ=255)
                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           >  1:5527797/1‑93 (MQ=255)
                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           >  1:1003753/1‑93 (MQ=255)
                                                gCTGGCTAACGGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGaa                                         <  1:2173554/93‑1 (MQ=255)
                                                gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGaa                                         >  2:7357106/1‑93 (MQ=255)
                                                 cTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAAc                                        <  1:6139035/93‑1 (MQ=255)
                                                     cTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcg                                    <  2:5942965/93‑1 (MQ=255)
                                                      tAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   >  1:3338571/1‑93 (MQ=255)
                                                      tAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   >  1:4361007/1‑93 (MQ=255)
                                                      tAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGCGACCAgcgc                                   >  1:3904348/1‑93 (MQ=255)
                                                           gAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACCGAGCcagca                              >  2:1081383/1‑93 (MQ=255)
                                                           gAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagca                              <  1:2303458/93‑1 (MQ=255)
                                                            accccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             <  2:3408817/91‑1 (MQ=255)
                                                             aCCCGCGCCACGGGCGGCTTCGGCAGCAG‑CTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGt                           >  2:1199759/1‑93 (MQ=255)
                                                              cccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGt                           >  1:4952419/1‑93 (MQ=255)
                                                                  cgcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCCCTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaaa                       <  2:4770543/93‑1 (MQ=255)
                                                                   gcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaaaa                      <  1:3105072/93‑1 (MQ=255)
                                                                   gcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaaaa                      <  1:3441902/93‑1 (MQ=255)
                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAacac                     >  2:539890/1‑93 (MQ=255)
                                                                      aCGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCa                   >  1:776329/1‑93 (MQ=255)
                                                                      aCGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCa                   <  2:1737868/93‑1 (MQ=255)
                                                                      aCGGGCGCCTTCGGCAGCAGCTTGAGCGGGACTTCGGTGGTCACGCCGAGCGTACCTTCCGATCAGGCGAACACAGCCAGCAGGCCAAAAcaa                   >  2:2748105/1‑91 (MQ=255)
                                                                       cGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAg                  >  2:7650675/1‑93 (MQ=255)
                                                                        gggCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAgg                 <  1:5661729/93‑1 (MQ=255)
                                                                        cggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAgg                 <  2:4626554/92‑1 (MQ=255)
                                                                          gcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTg               <  1:5207833/93‑1 (MQ=255)
                                                                           cggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGa              <  2:7776009/93‑1 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:5374952/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:1593779/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  2:7099854/1‑93 (MQ=255)
                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:5289507/1‑93 (MQ=255)
                                                                              cTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           <  1:3567852/93‑1 (MQ=255)
                                                                               ttCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          >  2:577890/1‑93 (MQ=255)
                                                                               ttCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          >  2:6512175/1‑93 (MQ=255)
                                                                               ttCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          >  2:421244/1‑93 (MQ=255)
                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  1:5978958/93‑1 (MQ=255)
                                                                                  ggCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAgc       <  1:7480872/93‑1 (MQ=255)
                                                                                   gcagcaGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAgcg      <  2:6789734/93‑1 (MQ=255)
                                                                                   gcagcaGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAgcg      >  1:3766641/1‑93 (MQ=255)
                                                                                   gcagcaGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAgcg      >  2:40301/1‑93 (MQ=255)
                                                                                    cagcagCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAgcgc     <  1:7115086/93‑1 (MQ=255)
                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    <  1:1682403/93‑1 (MQ=255)
                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    <  2:1675626/93‑1 (MQ=255)
                                                                                       cagCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTc  <  2:1893644/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  NC_000913/3125266‑3125452
                                                                                             |                                                                                             
ATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGC                                                                                               >  SRR4302543.5030792/1‑94 (MQ=60)
ATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGC                                                                                               >  SRR4302543.337729/1‑94 (MQ=60)
ATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGC                                                                                               >  SRR4302543.3012221/1‑94 (MQ=60)
ATTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGC                                                                                               >  SRR4302543.483804/1‑94 (MQ=60)
 TTGGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCT                                                                                              <  SRR4302543.4940582/94‑1 (MQ=60)
   GGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTT                                                                                            <  SRR4302543.1831036/94‑1 (MQ=60)
   GGCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTT                                                                                            <  SRR4302543.739608/94‑1 (MQ=60)
     CGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAC                                                                                          <  SRR4302543.2539261/94‑1 (MQ=60)
     CGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAC                                                                                          >  SRR4302543.6069244/1‑94 (MQ=60)
     CGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAC                                                                                          <  SRR4302543.6774652/94‑1 (MQ=60)
     CGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAC                                                                                          >  SRR4302543.2900385/1‑94 (MQ=60)
      gatgattgctCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         <  SRR4302543.7439198/84‑1 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.2731968/1‑94 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.2423869/1‑94 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.90681/1‑94 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         <  SRR4302543.2629758/94‑1 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.6875896/1‑94 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.6535861/1‑94 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.6875895/1‑94 (MQ=60)
      GATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCAGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.6899159/1‑94 (MQ=60)
      GATGATATCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCATGGGCAGCTTCGGCAGCAGCTTTACC                                                                                         >  SRR4302543.6899158/1‑94 (MQ=60)
       ATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCG                                                                                        >  SRR4302543.1729939/1‑94 (MQ=60)
        TGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGT                                                                                       >  SRR4302543.1910674/1‑94 (MQ=60)
        TGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGT                                                                                       >  SRR4302543.4613527/1‑94 (MQ=60)
        TGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGT                                                                                       >  SRR4302543.1547422/1‑94 (MQ=60)
         GATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTC                                                                                      <  SRR4302543.2610317/94‑1 (MQ=60)
         GATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTC                                                                                      <  SRR4302543.4218360/94‑1 (MQ=60)
          ATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCA                                                                                     >  SRR4302543.1526678/1‑94 (MQ=60)
          ATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCA                                                                                     <  SRR4302543.2607280/94‑1 (MQ=60)
            GTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACT                                                                                   >  SRR4302543.5830041/1‑94 (MQ=60)
            GTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACT                                                                                   >  SRR4302543.4946119/1‑94 (MQ=60)
             TCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTT                                                                                  <  SRR4302543.4327606/94‑1 (MQ=60)
              CACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTC                                                                                 >  SRR4302543.3230986/1‑94 (MQ=60)
              CACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTC                                                                                 <  SRR4302543.6907796/94‑1 (MQ=60)
              CACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTC                                                                                 >  SRR4302543.7437486/1‑94 (MQ=60)
                CCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGG                                                                               >  SRR4302543.5385391/1‑94 (MQ=60)
                CCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGG                                                                               >  SRR4302543.7504031/1‑94 (MQ=60)
                 CAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGT                                                                              >  SRR4302543.5643986/1‑94 (MQ=60)
                 CAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGT                                                                              <  SRR4302543.3544809/94‑1 (MQ=60)
                 CAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGT                                                                              >  SRR4302543.6256375/1‑94 (MQ=60)
                 CAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGT                                                                              >  SRR4302543.2084100/1‑94 (MQ=60)
                 CAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGT                                                                              >  SRR4302543.1529573/1‑94 (MQ=60)
                  AACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTG                                                                             <  SRR4302543.7493488/94‑1 (MQ=60)
                   ACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGTGCCACGGGCGGCTTCGGCACCAGCTTTACCGTCACTTCGGTGG                                                                            <  SRR4302543.5561905/94‑1 (MQ=60)
                   ACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGG                                                                            >  SRR4302543.178895/1‑94 (MQ=60)
                     CGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTC                                                                          >  SRR4302543.6387488/1‑94 (MQ=60)
                     CGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTC                                                                          <  SRR4302543.7912773/94‑1 (MQ=60)
                     CGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTC                                                                          >  SRR4302543.6083774/1‑94 (MQ=60)
                     CGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTC                                                                          >  SRR4302543.7944080/1‑94 (MQ=60)
                      GCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCA                                                                         >  SRR4302543.967997/1‑94 (MQ=60)
                       ctagcCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAC                                                                        <  SRR4302543.6201724/89‑1 (MQ=60)
                       CAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAC                                                                        <  SRR4302543.5132601/94‑1 (MQ=60)
                       CAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAC                                                                        <  SRR4302543.3620372/94‑1 (MQ=60)
                       CAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAC                                                                        <  SRR4302543.4807856/94‑1 (MQ=60)
                       CAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAC                                                                        <  SRR4302543.757649/94‑1 (MQ=60)
                          gccgggcgTTTTCTACCCAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCC                                                                     <  SRR4302543.6045171/86‑1 (MQ=60)
                          GCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCC                                                                     <  SRR4302543.6045172/94‑1 (MQ=60)
                           CCCGGCTTTTTCTACCGAGTCAACGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTCCCGTCACTTCGCTGGTTGCGCCG                                                                    >  SRR4302543.4101132/1‑94 (MQ=60)
                           CCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCG                                                                    >  SRR4302543.5124097/1‑94 (MQ=60)
                           CCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCG                                                                    >  SRR4302543.6891980/1‑94 (MQ=60)
                           CCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCG                                                                    >  SRR4302543.6670071/1‑94 (MQ=60)
                           CCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCG                                                                    <  SRR4302543.7473014/94‑1 (MQ=60)
                            CCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGC                                                                   >  SRR4302543.1613358/1‑94 (MQ=60)
                            CCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGA                                                                   <  SRR4302543.2662482/94‑1 (MQ=60)
                            CCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGA                                                                   <  SRR4302543.2061174/94‑1 (MQ=60)
                             CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  <  SRR4302543.7920672/94‑1 (MQ=60)
                             CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  <  SRR4302543.471331/94‑1 (MQ=60)
                             CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  <  SRR4302543.7051223/94‑1 (MQ=60)
                             CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  >  SRR4302543.275477/1‑94 (MQ=60)
                             CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  <  SRR4302543.195261/94‑1 (MQ=60)
                             CGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAG                                                                  >  SRR4302543.941493/1‑94 (MQ=60)
                              GGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGC                                                                 <  SRR4302543.1706575/94‑1 (MQ=60)
                               GCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCA                                                                >  SRR4302543.5338391/1‑94 (MQ=60)
                                CTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCAT                                                               <  SRR4302543.6246597/94‑1 (MQ=60)
                                 TTTTTCTACCGAGTCAAAGCTGGCTAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATA                                                              <  SRR4302543.85372/94‑1 (MQ=60)
                                  TTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAC                                                             >  SRR4302543.4027777/1‑94 (MQ=60)
                                  TTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAC                                                             >  SRR4302543.3662310/1‑94 (MQ=60)
                                      CTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTC                                                         >  SRR4302543.917335/1‑94 (MQ=60)
                                      CTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTC                                                         >  SRR4302543.1771239/1‑94 (MQ=60)
                                       TACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCC                                                        <  SRR4302543.4239835/94‑1 (MQ=60)
                                       TACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCC                                                        <  SRR4302543.3851928/94‑1 (MQ=60)
                                       TACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCC                                                        >  SRR4302543.4864521/1‑94 (MQ=60)
                                        ACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCG                                                       >  SRR4302543.5803034/1‑94 (MQ=60)
                                        ACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCG                                                       <  SRR4302543.2006132/94‑1 (MQ=60)
                                        ACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGATTTACCGTCACTTCGGTGGTCACGCCGAGCATACCGTCCG                                                       >  SRR4302543.5816233/1‑94 (MQ=60)
                                         CCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGA                                                      >  SRR4302543.1248314/1‑94 (MQ=60)
                                          CGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGAT                                                     <  SRR4302543.3019065/94‑1 (MQ=60)
                                          CGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGAT                                                     <  SRR4302543.5809052/94‑1 (MQ=60)
                                           GAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATC                                                    <  SRR4302543.849281/94‑1 (MQ=60)
                                            AGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCC                                                   >  SRR4302543.1816139/1‑94 (MQ=60)
                                             gctAAAGCTGGCTAACACAACCCGCGCAACGGGCGGCTTCGGCAGCAGCCTTACCGGCACTTCGTTGGTCACGCCGAGCATACCTTCCGATCCG                                                  <  SRR4302543.342479/91‑1 (MQ=60)
                                             GTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCG                                                  <  SRR4302543.342682/94‑1 (MQ=60)
                                             GTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCG                                                  >  SRR4302543.1715289/1‑94 (MQ=60)
                                             GTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCG                                                  <  SRR4302543.5123811/94‑1 (MQ=60)
                                               CAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGT                                                >  SRR4302543.4243966/1‑94 (MQ=60)
                                               CAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGT                                                <  SRR4302543.5187845/94‑1 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               >  SRR4302543.790640/1‑94 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               >  SRR4302543.6689173/1‑94 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302543.1686267/94‑1 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               >  SRR4302543.5578010/1‑94 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               >  SRR4302543.1012628/1‑94 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302543.5267635/94‑1 (MQ=60)
                                                AAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCCGTGGTCACGCCGCGCATACCTTCCGATCCGGTG                                               >  SRR4302543.5578011/1‑94 (MQ=60)
                                                AAAGCTGGCTAAAAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTG                                               <  SRR4302543.4261677/94‑1 (MQ=60)
                                                 AAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGCGCATACCTTCCGATCCGGTGA                                              >  SRR4302543.2860512/1‑94 (MQ=60)
                                                 AAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGA                                              <  SRR4302543.2119275/94‑1 (MQ=60)
                                                  AGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAA                                             <  SRR4302543.6896974/94‑1 (MQ=60)
                                                   GCTGGCTAACGGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAAC                                            <  SRR4302543.2193026/94‑1 (MQ=60)
                                                    CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCATTTTTACCGTCACATCGGCGGCCACGACAAGCATACCTTTCAATCCGGTGAACA                                           >  SRR4302543.5280491/1‑94 (MQ=60)
                                                    CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACA                                           <  SRR4302543.6197184/94‑1 (MQ=60)
                                                    CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACA                                           <  SRR4302543.4752885/94‑1 (MQ=60)
                                                    CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACA                                           <  SRR4302543.6347210/94‑1 (MQ=60)
                                                    CTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACA                                           >  SRR4302543.3130375/1‑94 (MQ=60)
                                                        CTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGC                                       >  SRR4302543.3368648/1‑94 (MQ=60)
                                                        CTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGC                                       >  SRR4302543.4400519/1‑94 (MQ=60)
                                                        CTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGCGACCAGCGC                                       >  SRR4302543.3938819/1‑94 (MQ=60)
                                                         TAACAGCACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCC                                      <  SRR4302543.5465235/94‑1 (MQ=60)
                                                          AACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCA                                     <  SRR4302543.4620973/94‑1 (MQ=60)
                                                           ACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAG                                    <  SRR4302543.492977/94‑1 (MQ=60)
                                                            CAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTACGATCaggacaaacgcgccatc                                   >  SRR4302543.1440234/1‑77 (MQ=60)
                                                              GAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAG                                 <  SRR4302543.2324444/94‑1 (MQ=60)
                                                              GAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAG                                 <  SRR4302543.3970432/94‑1 (MQ=60)
                                                              GAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAG                                 <  SRR4302543.4877688/94‑1 (MQ=60)
                                                              GAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGACAGCAG                                 >  SRR4302543.7322800/1‑94 (MQ=60)
                                                               AACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGG                                <  SRR4302543.2550420/94‑1 (MQ=60)
                                                                ACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGT                               >  SRR4302543.4997521/1‑94 (MQ=60)
                                                                  CCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCA                             >  SRR4302543.6035479/1‑94 (MQ=60)
                                                                  CCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCA                             <  SRR4302543.7650755/94‑1 (MQ=60)
                                                                    GCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAA                           <  SRR4302543.4062807/94‑1 (MQ=60)
                                                                     CCCCACGGGCGGTTTGGGCAGCAGCTTTACCGTCACTTCGGTGGTTACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAA                          <  SRR4302543.5985710/94‑1 (MQ=60)
                                                                      GCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAC                         <  SRR4302543.3133003/94‑1 (MQ=60)
                                                                      GCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAC                         <  SRR4302543.3472721/94‑1 (MQ=60)
                                                                       CCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACC                        <  SRR4302543.6269680/94‑1 (MQ=60)
                                                                       CCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACC                        >  SRR4302543.2373946/1‑94 (MQ=60)
                                                                        CACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCA                       >  SRR4302543.783151/1‑94 (MQ=60)
                                                                         ACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAG                      <  SRR4302543.4946747/94‑1 (MQ=60)
                                                                          CGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGG                     >  SRR4302543.6199152/1‑94 (MQ=60)
                                                                           GGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGT                    <  SRR4302543.5713370/94‑1 (MQ=60)
                                                                           GGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGT                    <  SRR4302543.5152735/94‑1 (MQ=60)
                                                                             GCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGA                  <  SRR4302543.5254767/94‑1 (MQ=60)
                                                                               GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302543.5423642/1‑94 (MQ=60)
                                                                               GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302543.7079658/1‑94 (MQ=60)
                                                                               GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302543.5337258/1‑94 (MQ=60)
                                                                               GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302543.1608003/1‑94 (MQ=60)
                                                                               GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                <  SRR4302543.3950231/94‑1 (MQ=60)
                                                                               GGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAT                >  SRR4302543.3432095/1‑94 (MQ=60)
                                                                                 CTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCC              >  SRR4302543.6761780/1‑94 (MQ=60)
                                                                                 CTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCC              <  SRR4302543.3599406/94‑1 (MQ=60)
                                                                                 CTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCC              >  SRR4302543.7974806/1‑94 (MQ=60)
                                                                                 CTTCGGCAGCAGCTTTACCGTCACTTCGGGGGTCACGCCGAGCATACCTTCCGATCCGGGGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCA              >  SRR4302543.6820979/1‑94 (MQ=60)
                                                                                  TTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCA             >  SRR4302543.2289338/1‑94 (MQ=60)
                                                                                  TTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCA             >  SRR4302543.2289146/1‑94 (MQ=60)
                                                                                   TCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAG            <  SRR4302543.2020652/94‑1 (MQ=60)
                                                                                   TCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAG            <  SRR4302543.6033731/94‑1 (MQ=60)
                                                                                   TCGGCAGCAGCCTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAG            <  SRR4302543.3328775/94‑1 (MQ=60)
                                                                                    CGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGC           <  SRR4302543.4054260/94‑1 (MQ=60)
                                                                                    CGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGC           <  SRR4302543.1029296/94‑1 (MQ=60)
                                                                                     GGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCG          >  SRR4302543.1473889/1‑94 (MQ=60)
                                                                                     GGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCG          <  SRR4302543.7552483/94‑1 (MQ=60)
                                                                                     GGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCG          >  SRR4302543.3799798/1‑94 (MQ=60)
                                                                                      GCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGC         <  SRR4302543.3557257/94‑1 (MQ=60)
                                                                                       CAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCG        <  SRR4302543.7184204/94‑1 (MQ=60)
                                                                                       CAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCG        >  SRR4302543.6694991/1‑94 (MQ=60)
                                                                                        AGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGT       <  SRR4302543.1697500/94‑1 (MQ=60)
                                                                                        AGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGC       >  SRR4302543.705495/1‑94 (MQ=60)
                                                                                        AGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGC       >  SRR4302543.7504884/1‑94 (MQ=60)
                                                                                         GCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC      >  SRR4302543.7889224/1‑94 (MQ=60)
                                                                                         GCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC      <  SRR4302543.4968236/94‑1 (MQ=60)
                                                                                         GCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC      >  SRR4302543.3818165/1‑94 (MQ=60)
                                                                                          CAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCC     >  SRR4302543.3897761/1‑94 (MQ=60)
                                                                                           AGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCG    >  SRR4302543.6181262/1‑94 (MQ=60)
                                                                                           AGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCG    >  SRR4302543.6371231/1‑94 (MQ=60)
                                                                                           AGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCG    >  SRR4302543.6371232/1‑94 (MQ=60)
                                                                                             CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  SRR4302543.2568214/1‑94 (MQ=60)
                                                                                             CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  SRR4302543.2655830/1‑94 (MQ=60)
                                                                                             CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  SRR4302543.5020903/1‑94 (MQ=60)
                                                                                             CTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  <  SRR4302543.2563854/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATTGGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTCCGAT  >  NC_000913/3125266‑3125452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑