Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,129,641 C→T V261V (GTG→GTA)  b2983 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,129,6410CT99.6% 997.4 / NA 280V261V (GTG→GTA) b2983hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (143/135);  total (145/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

CAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGC  >  NC_000913/3129551‑3129730
                                                                                          |                                                                                         
 aaaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACt                                                                                        >  2:6973069/1‑93 (MQ=255)
 aaaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGGCCTAACACGATCCCCACCAATACt                                                                                        >  2:3991644/1‑93 (MQ=255)
  aaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtg                                                                                       >  1:6629723/1‑93 (MQ=255)
  aaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtg                                                                                       >  2:5991685/1‑93 (MQ=255)
   aCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtgt                                                                                      <  1:2387517/93‑1 (MQ=255)
      cGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGtgct                                                                                   <  1:7923077/93‑1 (MQ=255)
        cTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGtgctgc                                                                                 <  1:7446324/93‑1 (MQ=255)
        cTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGtgctgc                                                                                 >  2:5128637/1‑93 (MQ=255)
        cTGGCGTGCCGGCGCCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCTGCGGGTCCTAACACGATCCCCTCCAATACTGtgctgc                                                                                 <  2:1046449/93‑1 (MQ=255)
          ggCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAc                                                                               <  2:7824990/93‑1 (MQ=255)
           gCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACg                                                                              >  1:3951860/1‑93 (MQ=255)
           gCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTCTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACg                                                                              >  1:1264368/1‑93 (MQ=255)
            ggTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGa                                                                             <  1:2503255/92‑1 (MQ=255)
              ttCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGt                                                                           <  1:2404659/93‑1 (MQ=255)
              ttCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGt                                                                           >  1:3834967/1‑93 (MQ=255)
              ttCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGt                                                                           <  1:5977382/93‑1 (MQ=255)
              ttCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGt                                                                           >  2:1603165/1‑93 (MQ=255)
                 cggcggCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTAc                                                                        <  2:4931129/93‑1 (MQ=255)
                   gcggcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCCCGAGTTAcgc                                                                      <  2:3384306/93‑1 (MQ=255)
                   gcggcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTAcgc                                                                      >  1:4320981/1‑93 (MQ=255)
                     ggcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACgcgc                                                                    >  2:3027143/1‑93 (MQ=255)
                      gcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGcc                                                                   >  2:6935109/1‑93 (MQ=255)
                        gTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGa                                                                 >  2:4891451/1‑93 (MQ=255)
                         tCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGATACGCGCCGAc                                                                >  1:3488157/1‑93 (MQ=255)
                          cGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAcc                                                               <  1:7550579/93‑1 (MQ=255)
                          cGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAcc                                                               <  2:6658572/93‑1 (MQ=255)
                             agaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAcccgt                                                            >  2:4060310/1‑90 (MQ=255)
                             agaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGa                                                            >  2:5697425/1‑93 (MQ=255)
                             agaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGa                                                            <  2:6929961/93‑1 (MQ=255)
                              gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           <  1:7163600/93‑1 (MQ=255)
                              gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           >  2:4756430/1‑93 (MQ=255)
                              gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           >  1:3615698/1‑93 (MQ=255)
                              gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           <  1:2767199/93‑1 (MQ=255)
                              gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           >  2:6790971/1‑93 (MQ=255)
                              gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           <  1:1457692/93‑1 (MQ=255)
                               aaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTCTGCTGCACGAGTTACGCGCCGACCAGATg                                                          >  2:2609965/1‑93 (MQ=255)
                                 tGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGa                                                        <  2:1253868/93‑1 (MQ=255)
                                 tGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGa                                                        >  1:6935036/1‑93 (MQ=255)
                                 tGTCACGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGa                                                        <  2:2273238/93‑1 (MQ=255)
                                  gTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAg                                                       <  2:6370726/93‑1 (MQ=255)
                                  gTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAg                                                       >  2:6168695/1‑93 (MQ=255)
                                  gTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAg                                                       <  1:3067845/93‑1 (MQ=255)
                                   tCATGGCGATTTTAAATACCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGt                                                      <  2:5733964/93‑1 (MQ=255)
                                   tCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGt                                                      <  2:7931397/93‑1 (MQ=255)
                                   tCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGt                                                      <  2:5153036/93‑1 (MQ=255)
                                    cATGGCGATTTTAAATAGCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTg                                                     <  1:7034460/93‑1 (MQ=255)
                                     aTGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTgg                                                    <  2:6292296/93‑1 (MQ=255)
                                     aTGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTgg                                                    >  1:3267773/1‑93 (MQ=255)
                                     aTGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTgg                                                    >  2:2682304/1‑93 (MQ=255)
                                     aTGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTgg                                                    <  2:4420172/93‑1 (MQ=255)
                                     aTGGCCATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTgg                                                    <  2:457588/93‑1 (MQ=255)
                                         cGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCaa                                                >  2:6861124/1‑93 (MQ=255)
                                          gATTTTAAATAACCCGGCGGCAGCGTGTCCTAACACAATCCCCACCAATACTGTGCTGCACGAGTTACGCGGCGACCAGATGGAGTGGGGAAt                                               >  2:4504907/1‑93 (MQ=255)
                                          gATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAAt                                               >  2:1976215/1‑93 (MQ=255)
                                          gATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAAt                                               <  1:268545/93‑1 (MQ=255)
                                           aTTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATg                                              <  2:7410408/93‑1 (MQ=255)
                                            ttttTAATAAGCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgt                                             >  2:5090229/1‑93 (MQ=255)
                                            ttttAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgt                                             <  1:5077136/93‑1 (MQ=255)
                                             tttAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgtt                                            >  2:5354717/1‑93 (MQ=255)
                                              ttAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttg                                           >  2:3931796/1‑93 (MQ=255)
                                                aaaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggt                                         >  1:7750959/1‑93 (MQ=255)
                                                aaaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggt                                         <  2:6446857/93‑1 (MQ=255)
                                                 aaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggtt                                        <  2:3701463/93‑1 (MQ=255)
                                                 aaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggtt                                        <  2:7542697/93‑1 (MQ=255)
                                                    aaCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAc                                     <  1:2195159/93‑1 (MQ=255)
                                                        cggcggCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGa                                 <  2:7077189/93‑1 (MQ=255)
                                                         ggcggcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGAc                                >  2:3262319/1‑93 (MQ=255)
                                                            ggcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaa                             >  1:4251481/1‑93 (MQ=255)
                                                             gcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaaa                            <  2:6140915/93‑1 (MQ=255)
                                                             gcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaaa                            >  2:2550906/1‑93 (MQ=255)
                                                               aGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCTCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACt                          >  1:5340077/1‑93 (MQ=255)
                                                               aGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACt                          <  2:3437499/93‑1 (MQ=255)
                                                                gCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTc                         <  2:3603852/93‑1 (MQ=255)
                                                                gCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTc                         <  1:6089939/93‑1 (MQ=255)
                                                                gCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAACAGACAAAGCTc                         >  1:3450867/1‑93 (MQ=255)
                                                                  gggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCa                       <  1:2111843/93‑1 (MQ=255)
                                                                    gTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAcg                     >  2:2205189/1‑93 (MQ=255)
                                                                     tCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAcgc                    <  1:7831595/93‑1 (MQ=255)
                                                                     gccTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAcgc                    <  1:6405776/92‑1 (MQ=255)
                                                                        caaCACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGcc                 <  2:1725640/92‑1 (MQ=255)
                                                                          acacGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCtt               <  1:5109688/93‑1 (MQ=255)
                                                                           cacGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCttt              >  2:559057/1‑93 (MQ=255)
                                                                              gATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAAt           <  2:3294418/93‑1 (MQ=255)
                                                                              gATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAAt           >  1:1980569/1‑93 (MQ=255)
                                                                                tCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGt         >  2:2826190/1‑93 (MQ=255)
                                                                                tCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGt         >  1:5725978/1‑93 (MQ=255)
                                                                                tCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGt         >  2:4390733/1‑93 (MQ=255)
                                                                                 ccccACCAATACTGTGCTGCACGAGTTACGCGCCGACCATATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTa        <  2:5888303/93‑1 (MQ=255)
                                                                                 ccccACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTa        <  1:5022473/93‑1 (MQ=255)
                                                                                  cccACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTAt       >  1:1047131/1‑93 (MQ=255)
                                                                                   ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      <  1:4745985/93‑1 (MQ=255)
                                                                                   ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      <  1:6797436/93‑1 (MQ=255)
                                                                                    caccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCg     <  2:942130/93‑1 (MQ=255)
                                                                                    caccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCg     <  1:5459943/93‑1 (MQ=255)
                                                                                    caccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCg     <  1:1109699/93‑1 (MQ=255)
                                                                                     cccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCgg    <  1:1751353/92‑1 (MQ=255)
                                                                                     accaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCgg    <  1:2861443/93‑1 (MQ=255)
                                                                                      ccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCggg   >  1:6536368/1‑93 (MQ=255)
                                                                                       caATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGc  >  2:3068536/1‑93 (MQ=255)
                                                                                       caATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGc  <  1:5548230/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGC  >  NC_000913/3129551‑3129730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  >  NC_000913/3129548‑3129734
                                                                                             |                                                                                             
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAAT                                                                                               <  SRR4302543.7549601/94‑1 (MQ=60)
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAAT                                                                                               <  SRR4302543.1257898/94‑1 (MQ=60)
 AGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATA                                                                                              >  SRR4302543.3575925/1‑94 (MQ=60)
 AGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATA                                                                                              >  SRR4302543.5648634/1‑94 (MQ=60)
  GCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATAC                                                                                             <  SRR4302543.2560586/94‑1 (MQ=60)
   CAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACT                                                                                            >  SRR4302543.1849614/1‑94 (MQ=60)
    AAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTG                                                                                           >  SRR4302543.463498/1‑94 (MQ=60)
    AAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTG                                                                                           >  SRR4302543.6692268/1‑94 (MQ=60)
      ACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTG                                                                                         <  SRR4302543.2409132/94‑1 (MQ=60)
       CCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGC                                                                                        >  SRR4302543.6049909/1‑94 (MQ=60)
        CCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCT                                                                                       <  SRR4302543.8001292/94‑1 (MQ=60)
         CGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTG                                                                                      <  SRR4302543.7997492/94‑1 (MQ=60)
           CTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTTCA                                                                                    >  SRR4302543.6132401/1‑94 (MQ=60)
           CTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCA                                                                                    <  SRR4302543.7517682/94‑1 (MQ=60)
           CTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCA                                                                                    >  SRR4302543.7866342/1‑94 (MQ=60)
           CTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCA                                                                                    >  SRR4302543.6848668/1‑94 (MQ=60)
            TGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAC                                                                                   <  SRR4302543.5761317/94‑1 (MQ=60)
             GGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACG                                                                                  >  SRR4302543.5047585/1‑94 (MQ=60)
             GGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACG                                                                                  <  SRR4302543.5861102/94‑1 (MQ=60)
             GGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACG                                                                                  >  SRR4302543.3986822/1‑94 (MQ=60)
             GGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTCTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACG                                                                                  >  SRR4302543.1275688/1‑94 (MQ=60)
               GGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAG                                                                                <  SRR4302543.2525978/94‑1 (MQ=60)
               CGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAG                                                                                >  SRR4302543.2779450/1‑94 (MQ=60)
                GTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGT                                                                               >  SRR4302543.3868807/1‑94 (MQ=60)
                GTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAAAACTGTGCTGCACGAGT                                                                               >  SRR4302543.7095684/1‑94 (MQ=60)
                 TTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTT                                                                              <  SRR4302543.6032136/94‑1 (MQ=60)
                 TTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTT                                                                              <  SRR4302543.2426407/94‑1 (MQ=60)
                   GCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTAC                                                                            <  SRR4302543.7182822/94‑1 (MQ=60)
                     GGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGC                                                                          >  SRR4302543.4360212/1‑94 (MQ=60)
                     GGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGC                                                                          >  SRR4302543.1607268/1‑94 (MQ=60)
                     GGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGC                                                                          >  SRR4302543.1607269/1‑94 (MQ=60)
                      GCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCG                                                                         <  SRR4302543.6153571/94‑1 (MQ=60)
                        GGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCC                                                                       <  SRR4302543.3566960/94‑1 (MQ=60)
                        GGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCC                                                                       <  SRR4302543.3641866/94‑1 (MQ=60)
                          CGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGA                                                                     <  SRR4302543.2449167/94‑1 (MQ=60)
                          CGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGA                                                                     >  SRR4302543.7348370/1‑94 (MQ=60)
                           GTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAC                                                                    >  SRR4302543.3766935/1‑94 (MQ=60)
                           GTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAC                                                                    >  SRR4302543.5811050/1‑94 (MQ=60)
                           GTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGATACGCGCCGAC                                                                    >  SRR4302543.3519385/1‑94 (MQ=60)
                            TCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACC                                                                   >  SRR4302543.6524046/1‑94 (MQ=60)
                            TCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACC                                                                   <  SRR4302543.3977930/94‑1 (MQ=60)
                             CGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCA                                                                  <  SRR4302543.7622499/94‑1 (MQ=60)
                             CGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCA                                                                  >  SRR4302543.2757569/1‑94 (MQ=60)
                              GAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAG                                                                 >  SRR4302543.4911972/1‑94 (MQ=60)
                               AAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGA                                                                <  SRR4302543.5536802/94‑1 (MQ=60)
                                AGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAT                                                               >  SRR4302543.3647576/1‑94 (MQ=60)
                                AGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAT                                                               >  SRR4302543.2073705/1‑94 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              >  SRR4302543.7031120/1‑94 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              >  SRR4302543.5520074/1‑94 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              <  SRR4302543.1470846/94‑1 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              >  SRR4302543.2046207/1‑94 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              <  SRR4302543.7233190/94‑1 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              <  SRR4302543.2792380/94‑1 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              >  SRR4302543.7795875/1‑94 (MQ=60)
                                  AATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGG                                                             >  SRR4302543.5294682/1‑94 (MQ=60)
                                   ATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGA                                                            >  SRR4302543.5245135/1‑94 (MQ=60)
                                   ATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGA                                                            >  SRR4302543.7000269/1‑94 (MQ=60)
                                    TGTCATGGCGATTTTAAATAACCCGGCTGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAG                                                           >  SRR4302543.1835936/1‑94 (MQ=60)
                                    TGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAG                                                           <  SRR4302543.3450493/94‑1 (MQ=60)
                                     GTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCCGATGGcgg                                                          >  SRR4302543.6790310/1‑91 (MQ=60)
                                     GTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGT                                                          <  SRR4302543.2649149/94‑1 (MQ=60)
                                     GTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGT                                                          <  SRR4302543.6874311/94‑1 (MQ=60)
                                     GTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGT                                                          <  SRR4302543.3095438/94‑1 (MQ=60)
                                     GTCATGGCGATGTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGT                                                          <  SRR4302543.5091690/94‑1 (MQ=60)
                                      TCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTG                                                         >  SRR4302543.6458155/1‑94 (MQ=60)
                                       CATGGCGATTTTAAATAGCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGG                                                        <  SRR4302543.7102160/94‑1 (MQ=60)
                                       CATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGG                                                        >  SRR4302543.2238477/1‑94 (MQ=60)
                                       CATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGG                                                        >  SRR4302543.243654/1‑94 (MQ=60)
                                       CATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGG                                                        >  SRR4302543.3297300/1‑94 (MQ=60)
                                       CATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTCCACGAGTTACGCGCCGACCAGATGGAGTGG                                                        >  SRR4302543.6916131/1‑94 (MQ=60)
                                             GATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATG                                                  <  SRR4302543.2151772/94‑1 (MQ=60)
                                             GATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATG                                                  <  SRR4302543.270906/94‑1 (MQ=60)
                                               TTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTT                                                <  SRR4302543.5123196/94‑1 (MQ=60)
                                                 TTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGG                                              <  SRR4302543.3486635/94‑1 (MQ=60)
                                                  TAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGT                                             <  SRR4302543.432779/94‑1 (MQ=60)
                                                  TAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGT                                             >  SRR4302543.7824150/1‑94 (MQ=60)
                                                     ATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGA                                          >  SRR4302543.2975177/1‑94 (MQ=60)
                                                      TAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAC                                         <  SRR4302543.7946294/94‑1 (MQ=60)
                                                       AACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACC                                        <  SRR4302543.2214884/94‑1 (MQ=60)
                                                           CGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGAC                                    <  SRR4302543.4493616/94‑1 (MQ=60)
                                                           CGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGAC                                    <  SRR4302543.7205824/94‑1 (MQ=60)
                                                              CGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAA                                 >  SRR4302543.4290070/1‑94 (MQ=60)
                                                               GGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAA                                >  SRR4302543.3292128/1‑94 (MQ=60)
                                                                GCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAAC                               >  SRR4302543.229756/1‑94 (MQ=60)
                                                                GCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGGGGGCAATGTTGGTTGACCAGACAAAAC                               <  SRR4302543.3148584/94‑1 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCTCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              >  SRR4302543.5388383/1‑94 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              <  SRR4302543.7614021/94‑1 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              >  SRR4302543.7565730/1‑94 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              >  SRR4302543.2873584/1‑94 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              <  SRR4302543.3930448/94‑1 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              <  SRR4302543.5098095/94‑1 (MQ=60)
                                                                  AGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTC                             <  SRR4302543.5139325/94‑1 (MQ=60)
                                                                  AGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAACAGACAAAGCTC                             >  SRR4302543.3481771/1‑94 (MQ=60)
                                                                   GCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCC                            <  SRR4302543.6147660/94‑1 (MQ=60)
                                                                    CGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCA                           <  SRR4302543.6641235/94‑1 (MQ=60)
                                                                     GGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAC                          <  SRR4302543.2130747/94‑1 (MQ=60)
                                                                     GGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAC                          <  SRR4302543.7863231/94‑1 (MQ=60)
                                                                     GGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAC                          <  SRR4302543.2877651/94‑1 (MQ=60)
                                                                     GGGTCCTAACACGATCCCCACCAATACGGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAC                          <  SRR4302543.2636559/94‑1 (MQ=60)
                                                                      GGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACG                         <  SRR4302543.1343608/94‑1 (MQ=60)
                                                                      GGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACG                         >  SRR4302543.1129306/1‑94 (MQ=60)
                                                                        TCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCG                       <  SRR4302543.7905333/94‑1 (MQ=60)
                                                                        TCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACCCCACGCG                       <  SRR4302543.2414887/94‑1 (MQ=60)
                                                                        GCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCG                       <  SRR4302543.6466104/94‑1 (MQ=60)
                                                                           TAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCT                    <  SRR4302543.7319444/94‑1 (MQ=60)
                                                                            AACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTT                   >  SRR4302543.7984647/1‑94 (MQ=60)
                                                                             ACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTT                  <  SRR4302543.5155924/94‑1 (MQ=60)
                                                                                CGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAAT               >  SRR4302543.1998319/1‑94 (MQ=60)
                                                                                CGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAAT               >  SRR4302543.334101/1‑94 (MQ=60)
                                                                                 GATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATG              <  SRR4302543.5493674/94‑1 (MQ=60)
                                                                                  ATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGT             >  SRR4302543.5778227/1‑94 (MQ=60)
                                                                                   TCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTA            >  SRR4302543.3757225/1‑94 (MQ=60)
                                                                                    CCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTAT           >  SRR4302543.1056508/1‑94 (MQ=60)
                                                                                    CCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTAT           <  SRR4302543.5068178/94‑1 (MQ=60)
                                                                                     CCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATC          <  SRR4302543.125076/94‑1 (MQ=60)
                                                                                     CCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATC          >  SRR4302543.5727002/1‑94 (MQ=60)
                                                                                      CCACCAATACTGTGCTGCACGAGTTACGCGCCGCCCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCG         <  SRR4302543.1303355/94‑1 (MQ=60)
                                                                                      CCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCG         <  SRR4302543.6861628/94‑1 (MQ=60)
                                                                                      CCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCG         <  SRR4302543.4789363/94‑1 (MQ=60)
                                                                                      CCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCG         <  SRR4302543.4162147/94‑1 (MQ=60)
                                                                                       CACCAATAGTGTGCTGCACGAGTTACGGGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGG        <  SRR4302543.1119712/94‑1 (MQ=60)
                                                                                       CACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGG        <  SRR4302543.5509429/94‑1 (MQ=60)
                                                                                       CACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGG        <  SRR4302543.1119713/94‑1 (MQ=60)
                                                                                        CCCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       <  SRR4302543.1767000/94‑1 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGagagtggagatcggaagagcggttcagcaggaatgccgagaccgatctcgtatgc       >  SRR4302543.4029445/1‑39 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       >  SRR4302543.6597898/1‑94 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       <  SRR4302543.2887540/94‑1 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       >  SRR4302543.5482207/1‑94 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       >  SRR4302543.4348732/1‑94 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       >  SRR4302543.6597903/1‑94 (MQ=60)
                                                                                          CAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCA     >  SRR4302543.6612385/1‑94 (MQ=60)
                                                                                          CAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCA     <  SRR4302543.5598687/94‑1 (MQ=60)
                                                                                          CAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCA     <  SRR4302543.2210042/94‑1 (MQ=60)
                                                                                          CAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCA     <  SRR4302543.2897041/94‑1 (MQ=60)
                                                                                          CAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCA     >  SRR4302543.1253781/1‑94 (MQ=60)
                                                                                           AATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAG    <  SRR4302543.5466704/94‑1 (MQ=60)
                                                                                           AATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAG    <  SRR4302543.2147427/94‑1 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACACAACTCCACGCGCCTTTAATGTATCGGGCAGA   >  SRR4302543.7995348/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTTTCGGGCAGA   >  SRR4302543.217093/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGC   >  SRR4302543.1897386/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGC   >  SRR4302543.1897387/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGA   >  SRR4302543.6036369/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGA   >  SRR4302543.2431709/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGA   >  SRR4302543.1646563/1‑94 (MQ=60)
                                                                                             TACTGTGCTGCACGAGTTACGCGCCGACTAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  >  SRR4302543.6273002/1‑94 (MQ=60)
                                                                                             TACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  <  SRR4302543.5807193/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  >  NC_000913/3129548‑3129734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑