Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,129,641 C→T V261V (GTG→GTA)  b2983 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,129,6410CT100.0% 534.0 / NA 149V261V (GTG→GTA) b2983hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (85/64);  total (85/64)

Only 100 of 160 total aligned reads displayed.

AAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGC  >  NC_000913/3129552‑3129730
                                                                                         |                                                                                         
aaaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTTAATAACCCTGCGGCAACGGGTCCTAACACGAACCCCACCAATACt                                                                                        >  2:2427235/1‑93 (MQ=255)
aaaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACt                                                                                        >  2:4597097/1‑93 (MQ=255)
aaaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACt                                                                                        <  1:2988237/93‑1 (MQ=255)
aaaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACt                                                                                        <  2:1527416/93‑1 (MQ=255)
 aaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtg                                                                                       >  1:1343920/1‑93 (MQ=255)
 aaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtg                                                                                       >  2:2651908/1‑93 (MQ=255)
 aaCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCAGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtg                                                                                       <  1:740859/93‑1 (MQ=255)
  aCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtgt                                                                                      >  1:1507637/1‑93 (MQ=255)
  aCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtgt                                                                                      <  1:2569077/93‑1 (MQ=255)
  aCCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGAACCCCACCAATACtgt                                                                                      <  1:2898417/93‑1 (MQ=255)
   cccGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACtgtg                                                                                     >  1:633418/1‑93 (MQ=255)
      gCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGtgctg                                                                                  <  1:1577034/93‑1 (MQ=255)
       cTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGtgctgc                                                                                 >  1:4099072/1‑93 (MQ=255)
       cTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGtgctgc                                                                                 >  1:1515809/1‑93 (MQ=255)
         ggCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAc                                                                               >  1:3285501/1‑93 (MQ=255)
            gTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAg                                                                            <  2:2190329/93‑1 (MQ=255)
            gTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAg                                                                            >  1:3920247/1‑93 (MQ=255)
             ttCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGt                                                                           >  1:2080525/1‑93 (MQ=255)
             ttCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGt                                                                           <  1:2719750/93‑1 (MQ=255)
              tCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGtt                                                                          >  2:4449244/1‑93 (MQ=255)
              tCCGGCGACGTCGAAGAATCTCATGGCGATTATAAATAACCAGGCGGCAGCGGGTCCTAACACGACCCCCACCAATACTGTGCTGCACGAGtt                                                                          <  1:4626232/93‑1 (MQ=255)
               ccGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTa                                                                         >  2:2858298/1‑93 (MQ=255)
                 ggcggcGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTAcg                                                                       >  2:4127790/1‑93 (MQ=255)
                 ggcggcGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTAcg                                                                       <  2:1058702/93‑1 (MQ=255)
                  gcggcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTAcgc                                                                      >  2:495017/1‑93 (MQ=255)
                    ggcgTCGAAGAATGTCATGGCGATTTTAAATACCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACgcgc                                                                    <  1:3199096/93‑1 (MQ=255)
                     gcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGcc                                                                   >  1:2553629/1‑93 (MQ=255)
                     gcgTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAAGAGCTACGCGcc                                                                   >  2:2452644/1‑93 (MQ=255)
                       gTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCgc                                                                 >  2:1199672/1‑92 (MQ=255)
                        tCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAc                                                                >  1:3155937/1‑93 (MQ=255)
                        tCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAc                                                                >  2:1970646/1‑93 (MQ=255)
                         cGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAcc                                                               <  2:3915705/93‑1 (MQ=255)
                           aagaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAg                                                             >  2:3534606/1‑93 (MQ=255)
                            agaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGa                                                            >  2:3991173/1‑93 (MQ=255)
                            agaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGa                                                            <  2:1941556/93‑1 (MQ=255)
                            agaaTGTCATGGCGATTTTAAATAACCCGGCGAAAGCGGGTCCTAACACGATCCCCACCAATACTGAGCTGCACCAGCTACGCGCCGAGCAGa                                                            >  2:96213/1‑93 (MQ=255)
                            agaaAGCCATGGCGATTTTAAATAACCCGGCGGGGGAGGGTCCTAACACGCTCCCCACCAATACTGTGCTGCATGAGGTACGCGCCGAACAgt                                                            >  2:2350795/1‑92 (MQ=255)
                             gaaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAt                                                           >  2:4023321/1‑93 (MQ=255)
                              aaTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATg                                                          >  1:3052137/1‑93 (MQ=255)
                               aTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATgg                                                         <  2:1758764/93‑1 (MQ=255)
                               aTGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATgg                                                         >  1:173587/1‑93 (MQ=255)
                                  tCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGt                                                      >  1:4527225/1‑93 (MQ=255)
                                  tCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGt                                                      <  1:999151/93‑1 (MQ=255)
                                    aTGGCAATTTTAAATAACCCGGCGGCATCGGGGCCTAACACGATCCCCACAAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTgg                                                    <  2:4495174/93‑1 (MQ=255)
                                       gCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGACGACCAGGTGGAGTGGGCa                                                 >  2:3022914/1‑93 (MQ=255)
                                         gATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAAt                                               <  1:4018477/93‑1 (MQ=255)
                                          aTTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATg                                              >  1:3558039/1‑93 (MQ=255)
                                           ttttAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgt                                             <  1:1837031/93‑1 (MQ=255)
                                           ttttAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgt                                             >  2:660941/1‑93 (MQ=255)
                                           ttttAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgt                                             >  2:4259210/1‑93 (MQ=255)
                                            tttAAATACCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGCGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgtt                                            <  1:2854573/93‑1 (MQ=255)
                                                aaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggtt                                        >  2:391744/1‑93 (MQ=255)
                                                aaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggtt                                        <  1:3634730/93‑1 (MQ=255)
                                                aaTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggtt                                        <  1:1956898/93‑1 (MQ=255)
                                                 aTAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATgttggttg                                       >  1:193315/1‑93 (MQ=255)
                                                   aaCCCGGCGGCAGCGGGTCCTAACACGATCCCCTCCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAc                                     <  2:3362023/93‑1 (MQ=255)
                                                   aaCCCGGCGGCAGCGGGTCCTAACACGATCCCCACAAATACTTTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAc                                     >  2:3643399/1‑93 (MQ=255)
                                                    aCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAcc                                    >  1:511256/1‑93 (MQ=255)
                                                    aCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAcc                                    >  2:2566118/1‑93 (MQ=255)
                                                     cccGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCa                                   <  2:1421218/93‑1 (MQ=255)
                                                     cccGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCa                                   <  1:1770645/93‑1 (MQ=255)
                                                       cggcggCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGa                                 <  2:4641422/93‑1 (MQ=255)
                                                         gcggcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACa                               >  2:2526709/1‑93 (MQ=255)
                                                           ggcAGCGGGACCTAACACGACCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaa                             <  2:3245579/93‑1 (MQ=255)
                                                            gcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaaa                            <  2:3737499/93‑1 (MQ=255)
                                                            gcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaaa                            >  2:2934377/1‑93 (MQ=255)
                                                            gcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaaa                            <  2:754139/93‑1 (MQ=255)
                                                            gcAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACaaaa                            <  1:1004140/93‑1 (MQ=255)
                                                              aGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACt                          <  1:3025980/93‑1 (MQ=255)
                                                              aGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAAGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACGAGACAAAAca                          >  1:2325696/1‑92 (MQ=255)
                                                               gCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTc                         >  1:1310930/1‑93 (MQ=255)
                                                               gCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTc                         >  2:3480974/1‑93 (MQ=255)
                                                                 gggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCa                       >  2:146238/1‑93 (MQ=255)
                                                                  ggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAc                      >  2:3661935/1‑93 (MQ=255)
                                                                  ggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAc                      <  1:2821136/93‑1 (MQ=255)
                                                                  ggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAc                      >  1:619334/1‑93 (MQ=255)
                                                                  ggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAc                      >  2:2097111/1‑93 (MQ=255)
                                                                  ggTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAc                      >  2:2097110/1‑93 (MQ=255)
                                                                        aaCACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCt                >  1:4092484/1‑93 (MQ=255)
                                                                          cacGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCttt              <  1:2998467/93‑1 (MQ=255)
                                                                          cacGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCttt              <  1:1964024/93‑1 (MQ=255)
                                                                             gATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAAt           <  1:1492305/93‑1 (MQ=255)
                                                                              aTCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATg          <  2:1708050/93‑1 (MQ=255)
                                                                               tCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGt         >  1:3728702/1‑93 (MQ=255)
                                                                               tCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGt         >  2:2784353/1‑93 (MQ=255)
                                                                               tCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGt         >  2:2710264/1‑93 (MQ=255)
                                                                                ccccACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTa        <  1:2577731/93‑1 (MQ=255)
                                                                                ccccACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTa        <  2:1124360/93‑1 (MQ=255)
                                                                                 cccACTAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTAt       <  1:383305/93‑1 (MQ=255)
                                                                                 cccACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAGGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTAt       <  1:1215439/93‑1 (MQ=255)
                                                                                  ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      >  2:4153419/1‑93 (MQ=255)
                                                                                  ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      >  1:868460/1‑93 (MQ=255)
                                                                                  ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      <  1:2929523/93‑1 (MQ=255)
                                                                                  ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      <  2:166431/93‑1 (MQ=255)
                                                                                  ccaccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATc      <  1:2379565/93‑1 (MQ=255)
                                                                                   caccaATACTGTGCTGCAAGAGCTCCGCCCAGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAAGCGCCTTTAATGTATCg     >  2:1280017/1‑93 (MQ=255)
                                                                                    cccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCgg    <  1:35355/92‑1 (MQ=255)
                                                                                    accaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTGGACCAGACAAAACTCCACGCGCCTTTAATGTATCgg    <  2:3190187/93‑1 (MQ=255)
                                                                                     ccaATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCggg   >  1:3723711/1‑93 (MQ=255)
                                                                                      caATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGc  >  2:2338680/1‑93 (MQ=255)
                                                                                         |                                                                                         
AAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGC  >  NC_000913/3129552‑3129730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  >  NC_000913/3129548‑3129734
                                                                                             |                                                                                             
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAAT                                                                                               <  SRR4302535.1171145/94‑1 (MQ=60)
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAAT                                                                                               <  SRR4302535.4102133/94‑1 (MQ=60)
 AGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATA                                                                                              >  SRR4302535.1985912/1‑94 (MQ=60)
    AAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTG                                                                                           <  SRR4302535.3009191/94‑1 (MQ=60)
    AAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTG                                                                                           >  SRR4302535.1353514/1‑94 (MQ=60)
     AACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGT                                                                                          >  SRR4302535.1518478/1‑94 (MQ=60)
     AACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCAGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGT                                                                                          <  SRR4302535.746064/94‑1 (MQ=60)
      ACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTG                                                                                         <  SRR4302535.2587556/94‑1 (MQ=60)
      ACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTG                                                                                         >  SRR4302535.637832/1‑94 (MQ=60)
      ACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTG                                                                                         <  SRR4302535.1800904/94‑1 (MQ=60)
      ACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGAACCCCACCAATACTGTG                                                                                         <  SRR4302535.2918914/94‑1 (MQ=60)
          GCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGC                                                                                     >  SRR4302535.1526728/1‑94 (MQ=60)
          GCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGC                                                                                     <  SRR4302535.1588415/94‑1 (MQ=60)
          GCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGC                                                                                     >  SRR4302535.4127137/1‑94 (MQ=60)
            TGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAC                                                                                   >  SRR4302535.3308339/1‑94 (MQ=60)
            TGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAC                                                                                   >  SRR4302535.535074/1‑94 (MQ=60)
               CGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAG                                                                                >  SRR4302535.3947054/1‑94 (MQ=60)
                GTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGT                                                                               >  SRR4302535.2095515/1‑94 (MQ=60)
                 TTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTT                                                                              <  SRR4302535.2739180/94‑1 (MQ=60)
                  TCCGGCGACGTCGAAGAATCTCATGGCGATTATAAATAACCAGGCGGCAGCGGGTCCTAACACGACCCCCACCAATACTGTGCTGCACGAGTTA                                                                             <  SRR4302535.4657640/94‑1 (MQ=60)
                    CGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACG                                                                           <  SRR4302535.3971678/94‑1 (MQ=60)
                    CGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACG                                                                           <  SRR4302535.2995652/94‑1 (MQ=60)
                        GGCGTCGAAGAATGTCATGGCGATTTTAAATACCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCC                                                                       <  SRR4302535.3221239/94‑1 (MQ=60)
                        GGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCC                                                                       >  SRR4302535.2572014/1‑94 (MQ=60)
                          CGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGA                                                                     >  SRR4302535.4287690/1‑94 (MQ=60)
                           GTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGAC                                                                    >  SRR4302535.3177854/1‑94 (MQ=60)
                             CGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCA                                                                  >  SRR4302535.123231/1‑94 (MQ=60)
                                AGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAT                                                               >  SRR4302535.1497182/1‑94 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              >  SRR4302535.3073513/1‑94 (MQ=60)
                                 GAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATG                                                              <  SRR4302535.24656/94‑1 (MQ=60)
                                  AATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGG                                                             >  SRR4302535.174786/1‑94 (MQ=60)
                                    TGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAG                                                           <  SRR4302535.3114239/94‑1 (MQ=60)
                                     GTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGT                                                          >  SRR4302535.4558232/1‑94 (MQ=60)
                                      TCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTG                                                         <  SRR4302535.1006237/94‑1 (MQ=60)
                                         TGGCGATTTTAAATAACCAGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATAGAGTGTGC                                                      >  SRR4302535.338320/1‑94 (MQ=60)
                                            CGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAAT                                                   <  SRR4302535.2592656/94‑1 (MQ=60)
                                             GATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATG                                                  >  SRR4302535.3582715/1‑94 (MQ=60)
                                             GATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATG                                                  <  SRR4302535.510961/94‑1 (MQ=60)
                                             GATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATG                                                  <  SRR4302535.4045972/94‑1 (MQ=60)
                                              ATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGT                                                 <  SRR4302535.2208384/94‑1 (MQ=60)
                                               TTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTT                                                <  SRR4302535.3775290/94‑1 (MQ=60)
                                               TTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTT                                                <  SRR4302535.1850284/94‑1 (MQ=60)
                                                TTTAAATACCCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGCGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTG                                               <  SRR4302535.2874794/94‑1 (MQ=60)
                                                   AAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTT                                            <  SRR4302535.1582977/94‑1 (MQ=60)
                                                   AAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTT                                            <  SRR4302535.2011196/94‑1 (MQ=60)
                                                    AATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTG                                           <  SRR4302535.3659917/94‑1 (MQ=60)
                                                    AATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTG                                           <  SRR4302535.1970983/94‑1 (MQ=60)
                                                    AATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTG                                           >  SRR4302535.194646/1‑94 (MQ=60)
                                                      TAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGAC                                         >  SRR4302535.319601/1‑94 (MQ=60)
                                                       AACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACC                                        >  SRR4302535.514831/1‑94 (MQ=60)
                                                         CCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAG                                      <  SRR4302535.1783477/94‑1 (MQ=60)
                                                               GGCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAA                                >  SRR4302535.2937549/1‑94 (MQ=60)
                                                                GCAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAAC                               <  SRR4302535.1011269/94‑1 (MQ=60)
                                                                 CAGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCAAGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACGAGACAAAACA                              >  SRR4302535.2342336/1‑94 (MQ=60)
                                                                 CAGCGGGTCCTAACACGAACCACACCAATTCTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACT                              <  SRR4302535.2855513/94‑1 (MQ=60)
                                                                  AGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTC                             <  SRR4302535.3047164/94‑1 (MQ=60)
                                                                  AGCGGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTC                             >  SRR4302535.1320300/1‑94 (MQ=60)
                                                                     GGGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCAC                          >  SRR4302535.623639/1‑94 (MQ=60)
                                                                      GGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACG                         <  SRR4302535.2841171/94‑1 (MQ=60)
                                                                      GGTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACG                         >  SRR4302535.69524/1‑94 (MQ=60)
                                                                       GTCCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGC                        >  SRR4302535.4095733/1‑94 (MQ=60)
                                                                         CCTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGC                      <  SRR4302535.1771571/94‑1 (MQ=60)
                                                                          CTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCC                     >  SRR4302535.949396/1‑94 (MQ=60)
                                                                          CTAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCC                     >  SRR4302535.3586688/1‑94 (MQ=60)
                                                                           TAACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCT                    >  SRR4302535.4120489/1‑94 (MQ=60)
                                                                             ACACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCACCTTT                  >  SRR4302535.1504424/1‑94 (MQ=60)
                                                                              CACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTA                 <  SRR4302535.3019493/94‑1 (MQ=60)
                                                                              CACGATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTA                 <  SRR4302535.1978184/94‑1 (MQ=60)
                                                                              CACGATACCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGCGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTA                 >  SRR4302535.3741845/1‑94 (MQ=60)
                                                                                 GATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATG              <  SRR4302535.1503025/94‑1 (MQ=60)
                                                                                 GATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATG              >  SRR4302535.3034298/1‑94 (MQ=60)
                                                                                  ATCCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGT             >  SRR4302535.3754527/1‑94 (MQ=60)
                                                                                    CCCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTAT           <  SRR4302535.2596254/94‑1 (MQ=60)
                                                                                     CCCACTAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATC          <  SRR4302535.385961/94‑1 (MQ=60)
                                                                                     CCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATC          >  SRR4302535.874605/1‑94 (MQ=60)
                                                                                     CCCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGAGGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATC          <  SRR4302535.1224156/94‑1 (MQ=60)
                                                                                      CCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCG         <  SRR4302535.2396653/94‑1 (MQ=60)
                                                                                      CCACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCG         <  SRR4302535.2950162/94‑1 (MQ=60)
                                                                                       CACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGG        <  SRR4302535.2649337/94‑1 (MQ=60)
                                                                                        CCCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       <  SRR4302535.35628/94‑1 (MQ=60)
                                                                                        ACCAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGG       >  SRR4302535.3749507/1‑94 (MQ=60)
                                                                                          CAATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCA     <  SRR4302535.1967904/94‑1 (MQ=60)
                                                                                           AATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAG    <  SRR4302535.694709/94‑1 (MQ=60)
                                                                                           AATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAG    >  SRR4302535.795194/1‑94 (MQ=60)
                                                                                            ATACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGA   >  SRR4302535.2203737/1‑94 (MQ=60)
                                                                                             TACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  <  SRR4302535.4466622/94‑1 (MQ=60)
                                                                                             TACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  <  SRR4302535.2459414/94‑1 (MQ=60)
                                                                                             |                                                                                             
CAGCAAACCCGCTGGCGTTCCGGCGGCGTCGAAGAATGTCATGGCGATTTTAAATAACCCGGCGGCAGCGGGTCCTAACACGATCCCCACCAACACTGTGCTGCACGAGTTACGCGCCGACCAGATGGAGTGGGCAATGTTGGTTGACCAGACAAAACTCCACGCGCCTTTAATGTATCGGGCAGAC  >  NC_000913/3129548‑3129734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑