Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,191,015 G→A G46S (GGT→AGT)  b3051 → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,191,0150GA98.2% 999.0 / ‑1.0 283G46S (GGT→AGT) b3051putative membrane protein
Reads supporting (aligned to +/- strand):  ref base G (4/1);  new base A (161/117);  total (165/118)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 290 total aligned reads displayed.

ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTG  >  NC_000913/3190925‑3191104
                                                                                          |                                                                                         
 ttattGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGtt                                                                                        <  2:6443256/93‑1 (MQ=255)
  tattGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGttt                                                                                       >  1:1352328/1‑93 (MQ=255)
   attGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTa                                                                                      <  1:7102018/93‑1 (MQ=255)
    ttGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAg                                                                                     >  1:6462950/1‑93 (MQ=255)
     tGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAgg                                                                                    <  2:6871996/93‑1 (MQ=255)
     tGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAgg                                                                                    <  1:3258568/93‑1 (MQ=255)
     tGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAgg                                                                                    <  2:285009/93‑1 (MQ=255)
      gCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGt                                                                                   >  2:2389316/1‑93 (MQ=255)
        cGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTgg                                                                                 <  1:5366090/93‑1 (MQ=255)
         gTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTggg                                                                                >  1:5841509/1‑93 (MQ=255)
         gTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTggg                                                                                >  1:3509505/1‑93 (MQ=255)
         gTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGCACTAGTTTAGGTggg                                                                                >  1:1792838/1‑93 (MQ=255)
          tATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTgggt                                                                               >  1:6713639/1‑92 (MQ=255)
          tATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGc                                                                               >  2:3913455/1‑93 (MQ=255)
            tGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCaa                                                                             >  2:3576985/1‑93 (MQ=255)
            tGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCaa                                                                             >  1:4174118/1‑93 (MQ=255)
            tGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCaa                                                                             >  1:7510965/1‑93 (MQ=255)
               aTTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCaaaaa                                                                          >  2:6014339/1‑93 (MQ=255)
                ttCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAg                                                                         >  1:495760/1‑93 (MQ=255)
                ttCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAg                                                                         >  2:5012453/1‑93 (MQ=255)
                ttCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAg                                                                         >  1:5676724/1‑93 (MQ=255)
                 tCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAgg                                                                        >  2:265173/1‑92 (MQ=255)
                  cTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTg                                                                       >  2:1631068/1‑93 (MQ=255)
                    gTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGt                                                                     >  1:820468/1‑93 (MQ=255)
                      ttattaTTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGCACTAGTTTAGGTGGGCAAAAAGTGGTaa                                                                   >  1:2003723/1‑93 (MQ=255)
                       tattatTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAAt                                                                  >  2:2719046/1‑93 (MQ=255)
                       tattatTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAAt                                                                  >  2:4583749/1‑93 (MQ=255)
                        attattGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATg                                                                 >  1:5481193/1‑93 (MQ=255)
                        atagttGGAAGTATTTTCGCCAGGCGCTGTCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATg                                                                 <  2:6316812/89‑1 (MQ=255)
                         ttattGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGa                                                                >  1:1190157/1‑93 (MQ=255)
                         ttattGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGa                                                                >  2:1990872/1‑93 (MQ=255)
                          tattGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAg                                                               >  2:808965/1‑93 (MQ=255)
                          tattGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAg                                                               >  1:108900/1‑93 (MQ=255)
                          tattGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAg                                                               <  1:2119124/93‑1 (MQ=255)
                             tGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCgg                                                            >  2:1886451/1‑93 (MQ=255)
                              ggAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGt                                                           <  2:6799566/93‑1 (MQ=255)
                               gAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTg                                                          <  2:7544623/93‑1 (MQ=255)
                                aaTTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTgg                                                         <  1:4774941/93‑1 (MQ=255)
                                 attattTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTggg                                                        >  2:7382868/1‑92 (MQ=255)
                                 attattTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGgc                                                        >  2:2446909/1‑93 (MQ=255)
                                  ttattTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGgcg                                                       >  1:7339310/1‑93 (MQ=255)
                                  ttattTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGgcg                                                       <  1:2911780/93‑1 (MQ=255)
                                     ttttCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCaa                                                    >  2:2953804/1‑93 (MQ=255)
                                      tttCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAAt                                                   >  2:735055/1‑93 (MQ=255)
                                       ttCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATc                                                  <  2:5351613/93‑1 (MQ=255)
                                         cGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGt                                                <  1:1936222/93‑1 (MQ=255)
                                          gCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTg                                               >  1:7686708/1‑93 (MQ=255)
                                           ccAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGa                                              <  2:6245405/93‑1 (MQ=255)
                                           ccAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGa                                              <  2:3325457/93‑1 (MQ=255)
                                            cAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGAt                                             >  1:4065071/1‑93 (MQ=255)
                                            cAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGAt                                             <  1:5290013/93‑1 (MQ=255)
                                            cAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGAt                                             >  1:4489785/1‑93 (MQ=255)
                                             aGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATg                                            >  2:594121/1‑93 (MQ=255)
                                              ggCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGc                                           >  1:2706156/1‑93 (MQ=255)
                                                 tctATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGa                                        >  1:1676653/1‑93 (MQ=255)
                                                   tATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATc                                      >  1:2475477/1‑93 (MQ=255)
                                                     tcgtcgCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCtt                                    >  1:517834/1‑93 (MQ=255)
                                                       gtcgCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTc                                  >  2:5826158/1‑93 (MQ=255)
                                                        tcgCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCa                                 <  1:3108027/93‑1 (MQ=255)
                                                        tcgCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCa                                 >  1:5957028/1‑93 (MQ=255)
                                                        tcgCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCa                                 >  1:6804938/1‑93 (MQ=255)
                                                         cgcgCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCAt                                >  1:4662398/1‑93 (MQ=255)
                                                          gcgcTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATg                               <  1:3012862/93‑1 (MQ=255)
                                                           cgcTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGa                              <  1:5086943/93‑1 (MQ=255)
                                                            gcTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGaa                             >  1:6431693/1‑93 (MQ=255)
                                                            gcTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGaa                             <  2:4194893/93‑1 (MQ=255)
                                                             cTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGaaa                            >  1:239645/1‑93 (MQ=255)
                                                             cTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGaaa                            >  1:2039071/1‑93 (MQ=255)
                                                             cTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGaaa                            <  1:4906758/93‑1 (MQ=255)
                                                                 ggCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAaataat                        >  2:3681006/1‑93 (MQ=255)
                                                                  gCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATc                       >  1:6932985/1‑93 (MQ=255)
                                                                  gCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATc                       >  1:3333884/1‑93 (MQ=255)
                                                                  gCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATc                       >  1:7219367/1‑93 (MQ=255)
                                                                  gCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATc                       <  1:1032938/93‑1 (MQ=255)
                                                                   cAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATcc                      <  2:4266054/93‑1 (MQ=255)
                                                                     gagCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATcccc                    <  1:4425951/93‑1 (MQ=255)
                                                                      agCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCa                   >  2:1273515/1‑93 (MQ=255)
                                                                      agCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCa                   <  2:5165052/93‑1 (MQ=255)
                                                                       gCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCAt                  >  2:5599765/1‑93 (MQ=255)
                                                                        cAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATc                 <  2:2612032/93‑1 (MQ=255)
                                                                        cAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATc                 <  1:1575254/93‑1 (MQ=255)
                                                                         aaGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCa                <  2:4062417/93‑1 (MQ=255)
                                                                         aaGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCa                >  2:2115727/1‑93 (MQ=255)
                                                                            cTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAata             <  1:3575745/93‑1 (MQ=255)
                                                                            cTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAata             <  2:6470738/93‑1 (MQ=255)
                                                                             ttttGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAatat            <  2:471812/93‑1 (MQ=255)
                                                                             ttttGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAatat            >  1:3376080/1‑93 (MQ=255)
                                                                             ttttGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAatat            >  1:3770918/1‑93 (MQ=255)
                                                                              tttGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATg           >  2:5459366/1‑93 (MQ=255)
                                                                              tttGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATg           >  1:286601/1‑93 (MQ=255)
                                                                               ttGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGa          <  2:5523677/93‑1 (MQ=255)
                                                                                tGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGaa         >  1:3469512/1‑93 (MQ=255)
                                                                                 gTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAAt        >  1:275259/1‑93 (MQ=255)
                                                                                  ttCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATa       <  1:7278550/93‑1 (MQ=255)
                                                                                  ttCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATa       <  1:2214681/93‑1 (MQ=255)
                                                                                  ttCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATa       >  1:6963477/1‑93 (MQ=255)
                                                                                  ttCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATa       <  2:1122825/93‑1 (MQ=255)
                                                                                   tCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATAc      <  2:4528991/93‑1 (MQ=255)
                                                                                   tCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATAc      <  2:7419008/93‑1 (MQ=255)
                                                                                     gTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATActc    <  1:1463863/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTG  >  NC_000913/3190925‑3191104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAG  >  NC_000913/3190923‑3191107
                                                                                            |                                                                                            
CAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAG                                                                                             >  SRR4302543.4408851/1‑94 (MQ=60)
 AATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGT                                                                                            >  SRR4302543.2483935/1‑94 (MQ=60)
 AATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGT                                                                                            >  SRR4302543.4703392/1‑94 (MQ=60)
 AATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGT                                                                                            >  SRR4302543.4260360/1‑94 (MQ=60)
  ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTT                                                                                           >  SRR4302543.3318048/1‑94 (MQ=60)
  ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTT                                                                                           <  SRR4302543.4842646/94‑1 (MQ=60)
  ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTT                                                                                           >  SRR4302543.7661498/1‑94 (MQ=60)
  ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGCACTGGTT                                                                                           >  SRR4302543.7554448/1‑94 (MQ=60)
  ATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCATACTAGTT                                                                                           >  SRR4302543.537313/1‑94 (MQ=60)
   TTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTT                                                                                          >  SRR4302543.3063588/1‑94 (MQ=60)
   TTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTT                                                                                          >  SRR4302543.1364424/1‑94 (MQ=60)
   TTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGATTTTGTTCGTACTAGTTT                                                                                          >  SRR4302543.1753496/1‑94 (MQ=60)
     ATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAG                                                                                        >  SRR4302543.6523847/1‑94 (MQ=60)
     ATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAG                                                                                        <  SRR4302543.7170998/94‑1 (MQ=60)
     ATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAG                                                                                        <  SRR4302543.1667608/94‑1 (MQ=60)
     ATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAG                                                                                        >  SRR4302543.6523846/1‑94 (MQ=60)
       TGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGT                                                                                      <  SRR4302543.4207883/94‑1 (MQ=60)
       TGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGT                                                                                      <  SRR4302543.3288022/94‑1 (MQ=60)
       TGCCGAATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGT                                                                                      >  SRR4302543.2247514/1‑94 (MQ=60)
        GCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTG                                                                                     >  SRR4302543.7050245/1‑94 (MQ=60)
        GCCGTATGCATTCTGTTGAGTATTGGATGTATGTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTG                                                                                     <  SRR4302543.4662715/94‑1 (MQ=60)
         CCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGG                                                                                    <  SRR4302543.1826037/94‑1 (MQ=60)
         CCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGG                                                                                    <  SRR4302543.7321361/94‑1 (MQ=60)
         CCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGG                                                                                    >  SRR4302543.6990436/1‑94 (MQ=60)
         CCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGG                                                                                    >  SRR4302543.4903981/1‑94 (MQ=60)
          CGTATGCATTTTGGTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   <  SRR4302543.2480384/94‑1 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   >  SRR4302543.3540871/1‑94 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   >  SRR4302543.5894652/1‑94 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   <  SRR4302543.5414667/94‑1 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   >  SRR4302543.607386/1‑94 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   >  SRR4302543.2798495/1‑94 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGG                                                                                   >  SRR4302543.6934043/1‑94 (MQ=60)
          CGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGCACTAGTTTAGGTGGG                                                                                   >  SRR4302543.1808882/1‑94 (MQ=60)
           GTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGT                                                                                  >  SRR4302543.6777080/1‑94 (MQ=60)
           GTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGA                                                                                  >  SRR4302543.6777085/1‑94 (MQ=60)
            TATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCA                                                                                 >  SRR4302543.1961965/1‑94 (MQ=60)
            TATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCA                                                                                 <  SRR4302543.5233544/94‑1 (MQ=60)
            TATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCA                                                                                 <  SRR4302543.5044279/94‑1 (MQ=60)
             ATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAA                                                                                <  SRR4302543.1787747/94‑1 (MQ=60)
             ATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAA                                                                                >  SRR4302543.4211920/1‑94 (MQ=60)
             ATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAA                                                                                >  SRR4302543.7582720/1‑94 (MQ=60)
              TGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAA                                                                               >  SRR4302543.5179025/1‑94 (MQ=60)
                 ATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTTGGCAAAAAG                                                                            >  SRR4302543.1679720/1‑94 (MQ=60)
                 ATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAG                                                                            >  SRR4302543.500344/1‑94 (MQ=60)
                 ATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAG                                                                            <  SRR4302543.439842/94‑1 (MQ=60)
                 ATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAG                                                                            >  SRR4302543.5728543/1‑94 (MQ=60)
                   TCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTG                                                                          >  SRR4302543.5167344/1‑94 (MQ=60)
                   TCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTG                                                                          <  SRR4302543.2907432/94‑1 (MQ=60)
                     TGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGT                                                                        >  SRR4302543.827692/1‑94 (MQ=60)
                     TGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGT                                                                        <  SRR4302543.452917/94‑1 (MQ=60)
                      GTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTA                                                                       >  SRR4302543.1968738/1‑94 (MQ=60)
                       TTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAA                                                                      <  SRR4302543.4732480/94‑1 (MQ=60)
                       TTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGCACTAGTTTAGGTGGGCAAAAAGTGGTAA                                                                      >  SRR4302543.2021722/1‑94 (MQ=60)
                        TTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAAT                                                                     <  SRR4302543.2956345/94‑1 (MQ=60)
                         TATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATG                                                                    >  SRR4302543.5530871/1‑94 (MQ=60)
                         TATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATG                                                                    <  SRR4302543.7938205/94‑1 (MQ=60)
                          ATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGA                                                                   >  SRR4302543.2793329/1‑94 (MQ=60)
                          ATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGA                                                                   >  SRR4302543.1200891/1‑94 (MQ=60)
                           TTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAG                                                                  >  SRR4302543.109894/1‑94 (MQ=60)
                           TTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAG                                                                  <  SRR4302543.2121765/94‑1 (MQ=60)
                            TATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGC                                                                 <  SRR4302543.5884465/94‑1 (MQ=60)
                            TATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGC                                                                 <  SRR4302543.2138094/94‑1 (MQ=60)
                             ATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCG                                                                >  SRR4302543.3011732/1‑94 (MQ=60)
                                GGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTG                                                             <  SRR4302543.374894/94‑1 (MQ=60)
                                  AATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGC                                                           <  SRR4302543.4818585/94‑1 (MQ=60)
                                  AATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGC                                                           <  SRR4302543.4818584/94‑1 (MQ=60)
                                   ATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCG                                                          >  SRR4302543.7409915/1‑94 (MQ=60)
                                   ATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCG                                                          >  SRR4302543.6505683/1‑94 (MQ=60)
                                   ATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCG                                                          >  SRR4302543.7626429/1‑94 (MQ=60)
                                    TTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGC                                                         >  SRR4302543.332458/1‑94 (MQ=60)
                                    TTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGC                                                         >  SRR4302543.7368247/1‑94 (MQ=60)
                                    TTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGC                                                         <  SRR4302543.2938242/94‑1 (MQ=60)
                                     TATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCA                                                        >  SRR4302543.5460226/1‑94 (MQ=60)
                                     TATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCA                                                        >  SRR4302543.7399003/1‑94 (MQ=60)
                                      ATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAA                                                       >  SRR4302543.5352759/1‑94 (MQ=60)
                                          TCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGT                                                   >  SRR4302543.3042345/1‑94 (MQ=60)
                                           CGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTG                                                  <  SRR4302543.1953628/94‑1 (MQ=60)
                                           CGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTG                                                  >  SRR4302543.1568881/1‑94 (MQ=60)
                                           CGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTG                                                  >  SRR4302543.7759500/1‑94 (MQ=60)
                                           CGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTG                                                  >  SRR4302543.5083445/1‑94 (MQ=60)
                                            GCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGA                                                 >  SRR4302543.2556303/1‑94 (MQ=60)
                                             CCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGAT                                                >  SRR4302543.4101479/1‑94 (MQ=60)
                                             CCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGAT                                                >  SRR4302543.4530578/1‑94 (MQ=60)
                                             CCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTGGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGAT                                                <  SRR4302543.497956/94‑1 (MQ=60)
                                              CAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATG                                               <  SRR4302543.5337767/94‑1 (MQ=60)
                                               AGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGC                                              >  SRR4302543.2730712/1‑94 (MQ=60)
                                                GGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCC                                             <  SRR4302543.1241258/94‑1 (MQ=60)
                                                GGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCC                                             <  SRR4302543.2911353/94‑1 (MQ=60)
                                                GGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCC                                             <  SRR4302543.1518615/94‑1 (MQ=60)
                                                 GCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCG                                            >  SRR4302543.7492273/1‑94 (MQ=60)
                                                  CTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGA                                           >  SRR4302543.1691681/1‑94 (MQ=60)
                                                  CTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGA                                           >  SRR4302543.4965007/1‑94 (MQ=60)
                                                   TCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGAT                                          >  SRR4302543.3149318/1‑94 (MQ=60)
                                                   TCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGAT                                          >  SRR4302543.729550/1‑94 (MQ=60)
                                                    CTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATC                                         >  SRR4302543.2497860/1‑94 (MQ=60)
                                                     TATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCT                                        >  SRR4302543.3909069/1‑94 (MQ=60)
                                                     TATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCT                                        >  SRR4302543.2767284/1‑94 (MQ=60)
                                                      ATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTT                                       >  SRR4302543.1817992/1‑94 (MQ=60)
                                                      ATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTT                                       >  SRR4302543.522639/1‑94 (MQ=60)
                                                         GTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCA                                    <  SRR4302543.5435469/94‑1 (MQ=60)
                                                         GTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCA                                    >  SRR4302543.6869191/1‑94 (MQ=60)
                                                         GTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCA                                    >  SRR4302543.6011552/1‑94 (MQ=60)
                                                          TCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCAT                                   >  SRR4302543.4704880/1‑94 (MQ=60)
                                                          TCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCAT                                   <  SRR4302543.3135986/94‑1 (MQ=60)
                                                          TCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCAT                                   <  SRR4302543.1151487/94‑1 (MQ=60)
                                                          TCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCAT                                   <  SRR4302543.6594375/94‑1 (MQ=60)
                                                           CGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATG                                  <  SRR4302543.2773239/94‑1 (MQ=60)
                                                           CGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATG                                  >  SRR4302543.7234078/1‑94 (MQ=60)
                                                           CGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATG                                  <  SRR4302543.2154653/94‑1 (MQ=60)
                                                            GCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGA                                 >  SRR4302543.4200111/1‑94 (MQ=60)
                                                            GCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGA                                 <  SRR4302543.3040136/94‑1 (MQ=60)
                                                             CGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAA                                <  SRR4302543.5133058/94‑1 (MQ=60)
                                                             CGCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAA                                >  SRR4302543.6492295/1‑94 (MQ=60)
                                                              GCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAA                               >  SRR4302543.1286542/1‑94 (MQ=60)
                                                              GCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAA                               >  SRR4302543.241719/1‑94 (MQ=60)
                                                              GCTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAA                               >  SRR4302543.2057367/1‑94 (MQ=60)
                                                               CTTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAAT                              <  SRR4302543.4951478/94‑1 (MQ=60)
                                                                TTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATA                             <  SRR4302543.6280366/94‑1 (MQ=60)
                                                                TTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATA                             >  SRR4302543.7000860/1‑94 (MQ=60)
                                                                TTCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATA                             <  SRR4302543.5713224/94‑1 (MQ=60)
                                                                 TCGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAA                            >  SRR4302543.1443609/1‑94 (MQ=60)
                                                                  gcGCAGAGCAAGCTTTTGTTCGTCCTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATCAAATAAT                           <  SRR4302543.2031961/92‑1 (MQ=60)
                                                                  CGGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAAT                           <  SRR4302543.2031963/94‑1 (MQ=60)
                                                                   GGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATC                          >  SRR4302543.3363931/1‑94 (MQ=60)
                                                                   GGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATC                          >  SRR4302543.6510731/1‑94 (MQ=60)
                                                                   GGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATC                          <  SRR4302543.6791279/94‑1 (MQ=60)
                                                                   GGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATC                          >  SRR4302543.3377495/1‑94 (MQ=60)
                                                                   GGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATC                          >  SRR4302543.7289341/1‑94 (MQ=60)
                                                                   GGCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATC                          >  SRR4302543.6998204/1‑94 (MQ=60)
                                                                    GCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCC                         >  SRR4302543.5786281/1‑94 (MQ=60)
                                                                    GCAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCC                         <  SRR4302543.1042109/94‑1 (MQ=60)
                                                                     CAGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCC                        >  SRR4302543.3545652/1‑94 (MQ=60)
                                                                     CAGAGCAAGCTTTTGTTCGTACTAGTTTAGGCGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCC                        >  SRR4302543.3399884/1‑94 (MQ=60)
                                                                      AGAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCC                       <  SRR4302543.2372334/94‑1 (MQ=60)
                                                                       GAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCA                      <  SRR4302543.2583252/94‑1 (MQ=60)
                                                                       GAGCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCA                      <  SRR4302543.4466054/94‑1 (MQ=60)
                                                                         GCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATC                    >  SRR4302543.3547529/1‑94 (MQ=60)
                                                                         GCAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATC                    <  SRR4302543.3953152/94‑1 (MQ=60)
                                                                          CAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCA                   >  SRR4302543.5895411/1‑94 (MQ=60)
                                                                          CAAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCA                   <  SRR4302543.1589310/94‑1 (MQ=60)
                                                                           GAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAA                  <  SRR4302543.2171401/94‑1 (MQ=60)
                                                                           AAGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAA                  >  SRR4302543.5287618/1‑94 (MQ=60)
                                                                            AGCTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAAT                 >  SRR4302543.7798440/1‑94 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               >  SRR4302543.1043243/1‑94 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               >  SRR4302543.3406383/1‑94 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               <  SRR4302543.1440222/94‑1 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               >  SRR4302543.3515607/1‑94 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               >  SRR4302543.3515611/1‑94 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               <  SRR4302543.3607345/94‑1 (MQ=60)
                                                                              CTTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATAT               >  SRR4302543.3804119/1‑94 (MQ=60)
                                                                               TTTTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATG              >  SRR4302543.289151/1‑94 (MQ=60)
                                                                                 TTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAA            >  SRR4302543.3764936/1‑94 (MQ=60)
                                                                                 TTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATGCCCATCAATATGAA            >  SRR4302543.3500589/1‑94 (MQ=60)
                                                                                 TTGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAA            >  SRR4302543.3500590/1‑94 (MQ=60)
                                                                                  TGTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAAT           >  SRR4302543.277690/1‑94 (MQ=60)
                                                                                   GTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGGCGATCTGTCATGAAATAATCCCCATCACTATGACTA          >  SRR4302543.7029007/1‑94 (MQ=60)
                                                                                   GTTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATA          >  SRR4302543.7029008/1‑94 (MQ=60)
                                                                                    TTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATAC         <  SRR4302543.7102250/94‑1 (MQ=60)
                                                                                    TTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATAC         <  SRR4302543.2234642/94‑1 (MQ=60)
                                                                                    TTCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATAC         <  SRR4302543.7348849/94‑1 (MQ=60)
                                                                                     TCGTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACT        <  SRR4302543.1180782/94‑1 (MQ=60)
                                                                                       GTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCT      <  SRR4302543.5923399/94‑1 (MQ=60)
                                                                                       GTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCT      <  SRR4302543.1477057/94‑1 (MQ=60)
                                                                                       GTACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCT      >  SRR4302543.3479330/1‑94 (MQ=60)
                                                                                        TACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTG     <  SRR4302543.932178/94‑1 (MQ=60)
                                                                                         ACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGA    >  SRR4302543.4454057/1‑94 (MQ=60)
                                                                                         ACTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGA    >  SRR4302543.4454058/1‑94 (MQ=60)
                                                                                          CTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAA   >  SRR4302543.2844777/1‑94 (MQ=60)
                                                                                          CTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAA   <  SRR4302543.3879838/94‑1 (MQ=60)
                                                                                          CTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAA   <  SRR4302543.7621369/94‑1 (MQ=60)
                                                                                          CTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAA   <  SRR4302543.5058074/94‑1 (MQ=60)
                                                                                          CTAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAA   >  SRR4302543.6716543/1‑94 (MQ=60)
                                                                                          CTAGTTGAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAA   <  SRR4302543.1374132/94‑1 (MQ=60)
                                                                                           TAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAG  <  SRR4302543.7409630/94‑1 (MQ=60)
                                                                                           TAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAG  <  SRR4302543.3667549/94‑1 (MQ=60)
                                                                                           TAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAG  <  SRR4302543.7510101/94‑1 (MQ=60)
                                                                                           TAGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAG  <  SRR4302543.1628947/94‑1 (MQ=60)
                                                                                            |                                                                                            
CAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTCGCGCTTCGGCAGAGCAAGCTTTTGTTCGTACTGGTTTAGGTGGGCAAAAAGTGGTAATGAGCGGTGGCGCAATCGTGATGCCGATCTTTCATGAAATAATCCCCATCAATATGAATACTCTGAAG  >  NC_000913/3190923‑3191107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑