Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,280 T→C L336P (CTG→CCG)  b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,2800TC99.4% 1053.7 / ‑4.3 338L336P (CTG→CCG) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (159/176);  total (161/177)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 358 total aligned reads displayed.

ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC  >  NC_000913/3221190‑3221369
                                                                                          |                                                                                         
  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       >  1:3414847/1‑93 (MQ=255)
  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       >  2:4082595/1‑93 (MQ=255)
  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       <  1:879490/93‑1 (MQ=255)
     cTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGc                                                                                    >  1:3570168/1‑93 (MQ=255)
     cTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGc                                                                                    <  1:1783819/93‑1 (MQ=255)
       ggAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGt                                                                                  <  1:5613403/93‑1 (MQ=255)
       ggAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGt                                                                                  <  2:5651734/93‑1 (MQ=255)
          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCaa                                                                               <  2:7408913/93‑1 (MQ=255)
           gtggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAAc                                                                              <  1:101651/93‑1 (MQ=255)
           gtggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAAc                                                                              <  1:4781564/93‑1 (MQ=255)
           gtggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAAc                                                                              <  1:3079849/93‑1 (MQ=255)
             ggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCg                                                                            >  1:1444462/1‑93 (MQ=255)
               tgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTc                                                                          <  1:6648952/93‑1 (MQ=255)
               tgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTc                                                                          >  2:4127015/1‑93 (MQ=255)
                gCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCa                                                                         <  1:436140/93‑1 (MQ=255)
                 ccACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAc                                                                        >  1:5178716/1‑93 (MQ=255)
                    caACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGAc                                                                     <  2:6020388/93‑1 (MQ=255)
                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                    >  1:7699296/1‑93 (MQ=255)
                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                    >  1:736726/1‑93 (MQ=255)
                      aCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACaa                                                                   <  2:5225342/93‑1 (MQ=255)
                      aCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACaa                                                                   <  1:1667859/93‑1 (MQ=255)
                       cgcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAAc                                                                  <  1:3893079/93‑1 (MQ=255)
                       cgcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGGGATGCCGCACGGCGTCAACCGTCACGACAAc                                                                  >  1:7886973/1‑93 (MQ=255)
                         cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGa                                                                <  2:4659397/93‑1 (MQ=255)
                         cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGa                                                                <  2:5506761/93‑1 (MQ=255)
                         cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGa                                                                >  1:3338860/1‑93 (MQ=255)
                          gTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGat                                                               <  1:5208846/93‑1 (MQ=255)
                          gTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGat                                                               <  1:60999/93‑1 (MQ=255)
                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                              >  1:1205370/1‑93 (MQ=255)
                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                              >  2:6391686/1‑93 (MQ=255)
                              gCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcatc                                                           <  2:2834057/93‑1 (MQ=255)
                                ttCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGc                                                         >  1:2266345/1‑93 (MQ=255)
                                ttCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGc                                                         >  1:2959462/1‑93 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      <  2:1529421/93‑1 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      <  2:2969604/93‑1 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      >  1:1599832/1‑93 (MQ=255)
                                    gTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAg                                                     >  1:535992/1‑93 (MQ=255)
                                       atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGcc                                                  <  2:1305895/93‑1 (MQ=255)
                                        tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg                                                 <  2:791626/93‑1 (MQ=255)
                                        tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg                                                 >  1:1882567/1‑93 (MQ=255)
                                        tatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcg                                                 <  1:3166069/93‑1 (MQ=255)
                                         atCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgc                                                >  2:3213041/1‑93 (MQ=255)
                                         atCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgc                                                <  2:7105884/93‑1 (MQ=255)
                                           cAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCgcgc                                              >  2:4750091/1‑93 (MQ=255)
                                            aaaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGcc                                             >  2:5812287/1‑93 (MQ=255)
                                             aaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCg                                            >  1:7900995/1‑93 (MQ=255)
                                              aGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGt                                           <  2:2664445/93‑1 (MQ=255)
                                              aGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGt                                           >  2:2106497/1‑93 (MQ=255)
                                              aGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGt                                           >  2:7549963/1‑93 (MQ=255)
                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                          <  1:6236552/93‑1 (MQ=255)
                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                          >  1:5562538/1‑93 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCATGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      >  1:4497057/1‑93 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      >  2:198615/1‑93 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      >  1:3678020/1‑93 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      <  2:3285458/93‑1 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                   >  2:671941/1‑93 (MQ=255)
                                                       cGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGa                                  <  2:3424660/93‑1 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  1:6412648/93‑1 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 >  2:4511841/1‑93 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  1:5759034/93‑1 (MQ=255)
                                                        cgTCTGTACTGGAACAATAACCGTTATGTGATGCCGCAGGGCGTCAAGCGTAACGACAACGATCATCGCAAAGGCCGCGCCGTGGGAATGGAt                                 <  2:4984902/92‑1 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  2:2504770/93‑1 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  1:4081428/93‑1 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  2:240239/93‑1 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                >  2:7268431/1‑93 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  2:5409841/93‑1 (MQ=255)
                                                          tCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcg                               >  2:4844365/1‑93 (MQ=255)
                                                           cTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcgc                              <  1:6585706/93‑1 (MQ=255)
                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCGCGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                            <  2:1703773/93‑1 (MQ=255)
                                                                cTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCga                         <  2:7644324/93‑1 (MQ=255)
                                                                  ggATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgaga                       >  2:5037473/1‑93 (MQ=255)
                                                                  ggATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgaga                       >  2:3082218/1‑93 (MQ=255)
                                                                  ggATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCgaga                       >  2:2018420/1‑93 (MQ=255)
                                                                     tCAATCACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGTCAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGCGaacg                    <  2:6994634/93‑3 (MQ=255)
                                                                     tCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                    >  2:6177505/1‑93 (MQ=255)
                                                                     tCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                    >  2:649299/1‑93 (MQ=255)
                                                                     tCAATAACCGTTATGTGATGCCGAACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAg                    <  2:4325700/93‑1 (MQ=255)
                                                                      cAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGa                   >  2:6759377/1‑93 (MQ=255)
                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                  <  2:3902719/93‑1 (MQ=255)
                                                                        aTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtc                 <  2:3777095/93‑1 (MQ=255)
                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGGtcc                >  2:2255189/1‑92 (MQ=255)
                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtct                >  1:4674070/1‑93 (MQ=255)
                                                                          aaCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctc               >  2:3041332/1‑93 (MQ=255)
                                                                          aaCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctc               <  2:1050608/93‑1 (MQ=255)
                                                                           aCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTcc              <  2:6837729/93‑1 (MQ=255)
                                                                             cGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg            <  2:3572778/93‑1 (MQ=255)
                                                                             cGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg            <  2:7357157/93‑1 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAGCCGTCAGGACAACGATCATCGCAAAGGCCGCTCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           <  2:1166289/93‑1 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           <  2:1834476/93‑1 (MQ=255)
                                                                               ttATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGtt          >  1:7234992/1‑93 (MQ=255)
                                                                               ttATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGtt          >  1:2640585/1‑93 (MQ=255)
                                                                               gtATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCTTTGGAATGGATCGCGTCGAGAAAGATCTCCAGtt          <  1:2466196/92‑1 (MQ=255)
                                                                                tATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtg         >  2:1954759/1‑93 (MQ=255)
                                                                                 aTGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtga        >  2:1417620/1‑93 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       <  1:6816056/93‑1 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       <  1:2026477/93‑1 (MQ=255)
                                                                                   gtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgatg      <  1:4890385/93‑1 (MQ=255)
                                                                                     gATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa    >  1:7135360/1‑93 (MQ=255)
                                                                                      aTGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAag   >  1:5840882/1‑93 (MQ=255)
                                                                                       tgccgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAagc  >  2:3465749/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC  >  NC_000913/3221190‑3221369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCA  >  NC_000913/3221187‑3221373
                                                                                             |                                                                                             
GCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCC                                                                                               >  SRR4302543.7427924/1‑94 (MQ=60)
 CAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCG                                                                                              >  SRR4302543.963107/1‑94 (MQ=60)
 CAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCG                                                                                              >  SRR4302543.7239880/1‑94 (MQ=60)
    CGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAC                                                                                           >  SRR4302543.3445415/1‑94 (MQ=60)
    CGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAC                                                                                           >  SRR4302543.6126271/1‑94 (MQ=60)
     GTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACG                                                                                          <  SRR4302543.989017/94‑1 (MQ=60)
     GTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACG                                                                                          <  SRR4302543.547498/94‑1 (MQ=60)
     GTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACG                                                                                          <  SRR4302543.887300/94‑1 (MQ=60)
     GTTCTGGAAGGGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACG                                                                                          <  SRR4302543.5911723/94‑1 (MQ=60)
      TTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGG                                                                                         <  SRR4302543.689094/94‑1 (MQ=60)
       TCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCGCGGC                                                                                        >  SRR4302543.838373/1‑94 (MQ=60)
       TCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGC                                                                                        >  SRR4302543.3601734/1‑94 (MQ=60)
        CTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGGCGCACGGCG                                                                                       >  SRR4302543.2246624/1‑94 (MQ=60)
        CTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCG                                                                                       <  SRR4302543.7357645/94‑1 (MQ=60)
        CTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCG                                                                                       <  SRR4302543.1799770/94‑1 (MQ=60)
        CTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCAGCACGGCG                                                                                       >  SRR4302543.3892832/1‑94 (MQ=60)
        CTGGAAGTGGTGCCACAACGCGTTGGCTTCCGAGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCG                                                                                       >  SRR4302543.3892833/1‑94 (MQ=60)
          GGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTC                                                                                     <  SRR4302543.5664552/94‑1 (MQ=60)
             AGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGAGCAATAACCGTTATGTGATGCCGCACGGCGTCAAC                                                                                  >  SRR4302543.4878868/1‑94 (MQ=60)
              GTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACC                                                                                 <  SRR4302543.5390776/94‑1 (MQ=60)
              GTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACC                                                                                 <  SRR4302543.3107544/94‑1 (MQ=60)
              GTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACC                                                                                 <  SRR4302543.102590/94‑1 (MQ=60)
              GTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACC                                                                                 <  SRR4302543.4818416/94‑1 (MQ=60)
              GTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACC                                                                                 <  SRR4302543.4825266/94‑1 (MQ=60)
               TGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCG                                                                                >  SRR4302543.1457511/1‑94 (MQ=60)
                  TGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCA                                                                             <  SRR4302543.6711713/94‑1 (MQ=60)
                  TCCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCA                                                                             <  SRR4302543.6711712/94‑1 (MQ=60)
                   GCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATACCCGTTATGTGATGCCGCACGGCGTCAACCGTCAC                                                                            <  SRR4302543.4073810/94‑1 (MQ=60)
                   GCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAC                                                                            <  SRR4302543.3296727/94‑1 (MQ=60)
                   GCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAC                                                                            >  SRR4302543.5225462/1‑94 (MQ=60)
                   GCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAC                                                                            <  SRR4302543.2444878/94‑1 (MQ=60)
                   GCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAC                                                                            <  SRR4302543.440228/94‑1 (MQ=60)
                    CCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACG                                                                           <  SRR4302543.7809064/94‑1 (MQ=60)
                       CAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACA                                                                        >  SRR4302543.7772167/1‑94 (MQ=60)
                       CAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACA                                                                        >  SRR4302543.743323/1‑94 (MQ=60)
                         ACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAAC                                                                      <  SRR4302543.1682800/94‑1 (MQ=60)
                         ACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGGGATGCCGCACGGCGTCAACCGTCACGACAAC                                                                      >  SRR4302543.7961170/1‑94 (MQ=60)
                          CGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACG                                                                     >  SRR4302543.6375327/1‑94 (MQ=60)
                          CGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACG                                                                     <  SRR4302543.3927451/94‑1 (MQ=60)
                          CGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACG                                                                     >  SRR4302543.6033629/1‑94 (MQ=60)
                          CGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACG                                                                     <  SRR4302543.2001714/94‑1 (MQ=60)
                           GCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGA                                                                    >  SRR4302543.3044815/1‑94 (MQ=60)
                           GCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGA                                                                    >  SRR4302543.3368938/1‑94 (MQ=60)
                             GTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATC                                                                  >  SRR4302543.6185561/1‑94 (MQ=60)
                             GTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATC                                                                  <  SRR4302543.61535/94‑1 (MQ=60)
                             GTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATC                                                                  >  SRR4302543.1216218/1‑94 (MQ=60)
                             GTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATC                                                                  <  SRR4302543.5255782/94‑1 (MQ=60)
                              TTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCA                                                                 <  SRR4302543.3657001/94‑1 (MQ=60)
                              TTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCA                                                                 <  SRR4302543.5715290/94‑1 (MQ=60)
                              TTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCA                                                                 <  SRR4302543.5715289/94‑1 (MQ=60)
                               TGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCAT                                                                <  SRR4302543.3451856/94‑1 (MQ=60)
                               TGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCAT                                                                <  SRR4302543.2850870/94‑1 (MQ=60)
                                GGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATC                                                               >  SRR4302543.4020577/1‑94 (MQ=60)
                                GGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATC                                                               >  SRR4302543.783318/1‑94 (MQ=60)
                                 GCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCG                                                              >  SRR4302543.4749271/1‑94 (MQ=60)
                                  CTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGC                                                             >  SRR4302543.2986332/1‑94 (MQ=60)
                                  CTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGC                                                             >  SRR4302543.2287032/1‑94 (MQ=60)
                                   TTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCA                                                            >  SRR4302543.1097748/1‑94 (MQ=60)
                                   TTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCA                                                            <  SRR4302543.4611934/94‑1 (MQ=60)
                                   TTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCA                                                            <  SRR4302543.950053/94‑1 (MQ=60)
                                   TTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCA                                                            >  SRR4302543.5235683/1‑94 (MQ=60)
                                     CCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAA                                                          >  SRR4302543.1614106/1‑94 (MQ=60)
                                      CGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAG                                                         <  SRR4302543.5628266/94‑1 (MQ=60)
                                      CGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAG                                                         <  SRR4302543.3899704/94‑1 (MQ=60)
                                      CGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAG                                                         <  SRR4302543.1378133/94‑1 (MQ=60)
                                      CGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAG                                                         <  SRR4302543.5882994/94‑1 (MQ=60)
                                      CGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAG                                                         >  SRR4302543.540960/1‑94 (MQ=60)
                                       GTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGG                                                        <  SRR4302543.13895/94‑1 (MQ=60)
                                       GTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGG                                                        <  SRR4302543.3129596/94‑1 (MQ=60)
                                       GTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGG                                                        <  SRR4302543.4627115/94‑1 (MQ=60)
                                        TGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGC                                                       >  SRR4302543.5278455/1‑94 (MQ=60)
                                        TGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGC                                                       >  SRR4302543.473642/1‑94 (MQ=60)
                                         GATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCC                                                      <  SRR4302543.6258135/94‑1 (MQ=60)
                                         GATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCC                                                      >  SRR4302543.1810337/1‑94 (MQ=60)
                                         GATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCC                                                      <  SRR4302543.552464/94‑1 (MQ=60)
                                          ATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTTTGTGGCGCCGGACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCG                                                     <  SRR4302543.5441628/94‑1 (MQ=60)
                                          ATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCG                                                     >  SRR4302543.5623175/1‑94 (MQ=60)
                                          ATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCG                                                     <  SRR4302543.5441629/94‑1 (MQ=60)
                                          ATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCG                                                     >  SRR4302543.1899485/1‑94 (MQ=60)
                                           TATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGC                                                    <  SRR4302543.3194574/94‑1 (MQ=60)
                                           TATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGACGC                                                    >  SRR4302543.1842053/1‑94 (MQ=60)
                                            ATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCG                                                   <  SRR4302543.6583079/94‑1 (MQ=60)
                                            ATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCG                                                   >  SRR4302543.3570873/1‑94 (MQ=60)
                                               AAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCG                                                >  SRR4302543.7975264/1‑94 (MQ=60)
                                               AAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCG                                                <  SRR4302543.5134957/94‑1 (MQ=60)
                                                 AGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTT                                              >  SRR4302543.683456/1‑94 (MQ=60)
                                                 AGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTT                                              >  SRR4302543.5613130/1‑94 (MQ=60)
                                                  GTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTG                                             <  SRR4302543.7763715/94‑1 (MQ=60)
                                                  GTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTG                                             <  SRR4302543.4855189/94‑1 (MQ=60)
                                                  GTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTG                                             <  SRR4302543.6295408/94‑1 (MQ=60)
                                                   tctGCGACGGTCTGGTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGG                                            <  SRR4302543.2691077/91‑1 (MQ=60)
                                                    GCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGA                                           <  SRR4302543.3442344/94‑1 (MQ=60)
                                                    GCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGA                                           <  SRR4302543.3280062/94‑1 (MQ=60)
                                                     CGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCATGACAACGATCATCGCAAAGGCCGCGCCGTTGGAA                                          >  SRR4302543.4537927/1‑94 (MQ=60)
                                                     CGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAA                                          >  SRR4302543.3686087/1‑94 (MQ=60)
                                                     CGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAA                                          >  SRR4302543.6597684/1‑94 (MQ=60)
                                                     CGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAA                                          >  SRR4302543.3710325/1‑94 (MQ=60)
                                                      GCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAT                                         <  SRR4302543.6244882/94‑1 (MQ=60)
                                                      GCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAT                                         <  SRR4302543.2289770/94‑1 (MQ=60)
                                                       CGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATG                                        <  SRR4302543.6382620/94‑1 (MQ=60)
                                                       CGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATG                                        >  SRR4302543.7527538/1‑94 (MQ=60)
                                                        GACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGG                                       <  SRR4302543.1291329/94‑1 (MQ=60)
                                                          CTGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAT                                     <  SRR4302543.163322/94‑1 (MQ=60)
                                                           GGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATC                                    <  SRR4302543.7394260/94‑1 (MQ=60)
                                                           GGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATC                                    <  SRR4302543.5811504/94‑1 (MQ=60)
                                                           GGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATC                                    <  SRR4302543.7391128/94‑1 (MQ=60)
                                                           GGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATC                                    <  SRR4302543.6473037/94‑1 (MQ=60)
                                                           GGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATC                                    <  SRR4302543.3600855/94‑1 (MQ=60)
                                                            GTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCG                                   <  SRR4302543.795503/94‑1 (MQ=60)
                                                            GTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCG                                   <  SRR4302543.302768/94‑1 (MQ=60)
                                                            GTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCG                                   <  SRR4302543.4118013/94‑1 (MQ=60)
                                                             TCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGC                                  >  SRR4302543.1998323/1‑94 (MQ=60)
                                                              CTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCG                                 <  SRR4302543.1492012/94‑1 (MQ=60)
                                                              CTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCG                                 <  SRR4302543.6647743/94‑1 (MQ=60)
                                                               TGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGT                                <  SRR4302543.7970543/94‑1 (MQ=60)
                                                                GTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTC                               >  SRR4302543.5205392/1‑94 (MQ=60)
                                                                 TTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCG                              >  SRR4302543.984106/1‑94 (MQ=60)
                                                                  TCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGA                             >  SRR4302543.4254150/1‑94 (MQ=60)
                                                                  TCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGA                             >  SRR4302543.2219726/1‑94 (MQ=60)
                                                                   CTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGta                            >  SRR4302543.1703120/1‑92 (MQ=60)
                                                                   CTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAG                            <  SRR4302543.5944623/94‑1 (MQ=60)
                                                                   CTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAG                            <  SRR4302543.5944624/94‑1 (MQ=60)
                                                                   CTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAG                            <  SRR4302543.4519690/94‑1 (MQ=60)
                                                                   CTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAA                            >  SRR4302543.1674018/1‑94 (MQ=60)
                                                                   CTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGGCACGACAACGATCATCGCAAACGCCGCGCCGTTGGAATGGATCGCGTCGAG                            >  SRR4302543.1674016/1‑94 (MQ=60)
                                                                    TGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGA                           >  SRR4302543.5729870/1‑94 (MQ=60)
                                                                      GATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAA                         >  SRR4302543.5853053/1‑94 (MQ=60)
                                                                      GATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAA                         >  SRR4302543.5071132/1‑94 (MQ=60)
                                                                       ATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAG                        >  SRR4302543.7266317/1‑94 (MQ=60)
                                                                         TAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAT                      <  SRR4302543.3182825/94‑1 (MQ=60)
                                                                         CAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAcac                      >  SRR4302543.2590843/1‑91 (MQ=60)
                                                                         CAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAT                      >  SRR4302543.824001/1‑94 (MQ=60)
                                                                         CAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAT                      <  SRR4302543.3183070/94‑1 (MQ=60)
                                                                          AATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATC                     >  SRR4302543.6737290/1‑94 (MQ=60)
                                                                           ATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCT                    <  SRR4302543.522730/94‑1 (MQ=60)
                                                                           ATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCT                    <  SRR4302543.3984069/94‑1 (MQ=60)
                                                                           ATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCT                    >  SRR4302543.4716682/1‑94 (MQ=60)
                                                                           ATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCT                    <  SRR4302543.2319895/94‑1 (MQ=60)
                                                                           ATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCT                    <  SRR4302543.4416800/94‑1 (MQ=60)
                                                                              ACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCA                 <  SRR4302543.1808682/94‑1 (MQ=60)
                                                                              ACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCA                 >  SRR4302543.7967343/1‑94 (MQ=60)
                                                                               CCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAG                <  SRR4302543.109306/94‑1 (MQ=60)
                                                                                CGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGT               <  SRR4302543.3421612/94‑1 (MQ=60)
                                                                                CGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGT               <  SRR4302543.3202381/94‑1 (MQ=60)
                                                                                CGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGT               >  SRR4302543.68250/1‑94 (MQ=60)
                                                                                CGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGT               >  SRR4302543.2791752/1‑94 (MQ=60)
                                                                                 GTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTT              >  SRR4302543.5244819/1‑94 (MQ=60)
                                                                                 GTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTT              <  SRR4302543.879560/94‑1 (MQ=60)
                                                                                 GTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTT              >  SRR4302543.7305049/1‑94 (MQ=60)
                                                                                 GTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTT              >  SRR4302543.2664502/1‑94 (MQ=60)
                                                                                  TTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTG             <  SRR4302543.2488456/94‑1 (MQ=60)
                                                                                  GTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCTTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTG             <  SRR4302543.2488455/94‑1 (MQ=60)
                                                                                    ATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGAT           <  SRR4302543.7201802/94‑1 (MQ=60)
                                                                                    ATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGAT           >  SRR4302543.419998/1‑94 (MQ=60)
                                                                                    ATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGAT           <  SRR4302543.7090590/94‑1 (MQ=60)
                                                                                     TGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGCTG          >  SRR4302543.447452/1‑94 (MQ=60)
                                                                                     TGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATG          <  SRR4302543.2044686/94‑1 (MQ=60)
                                                                                     TGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATG          <  SRR4302543.6880406/94‑1 (MQ=60)
                                                                                      GTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGA         >  SRR4302543.1582675/1‑94 (MQ=60)
                                                                                      GTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGA         <  SRR4302543.4934964/94‑1 (MQ=60)
                                                                                      GTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGA         <  SRR4302543.1690121/94‑1 (MQ=60)
                                                                                       TGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAA        <  SRR4302543.1973281/94‑1 (MQ=60)
                                                                                       TGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAA        >  SRR4302543.7280274/1‑94 (MQ=60)
                                                                                       TGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAA        >  SRR4302543.7204699/1‑94 (MQ=60)
                                                                                        GATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAG       >  SRR4302543.5894018/1‑94 (MQ=60)
                                                                                         ATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC      >  SRR4302543.6665754/1‑94 (MQ=60)
                                                                                          TGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGANAGATCTCCAGTTNANGNANCA     >  SRR4302543.595362/1‑94 (MQ=60)
                                                                                          TGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCA     >  SRR4302543.1583594/1‑94 (MQ=60)
                                                                                          TGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCA     >  SRR4302543.6741380/1‑94 (MQ=60)
                                                                                           GCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAG    <  SRR4302543.2023992/94‑1 (MQ=60)
                                                                                           GCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAG    >  SRR4302543.6314041/1‑94 (MQ=60)
                                                                                            CCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGC   <  SRR4302543.3340800/94‑1 (MQ=60)
                                                                                            CCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGC   >  SRR4302543.6262374/1‑94 (MQ=60)
                                                                                             CGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCA  <  SRR4302543.781909/94‑1 (MQ=60)
                                                                                             CGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCA  >  SRR4302543.6339719/1‑94 (MQ=60)
                                                                                             CGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCA  <  SRR4302543.3225199/94‑1 (MQ=60)
                                                                                             CGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCA  <  SRR4302543.949589/94‑1 (MQ=60)
                                                                                             |                                                                                             
GCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCA  >  NC_000913/3221187‑3221373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑