Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,253,781 T→C E171G (GAG→GGG)  yhaNM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,253,7810TC99.2% 813.7 / ‑5.1 260E171G (GAG→GGG) yhaNMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (139/119);  minor base A (0/2);  total (139/121)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253690‑3253870
                                                                                           |                                                                                         
  cgccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGc                                                                                       >  2:5450251/1‑93 (MQ=255)
      gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcaca                                                                                   >  1:651083/1‑93 (MQ=255)
       cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                                                                  <  1:7803305/93‑1 (MQ=255)
       cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacac                                                                                  >  1:883321/1‑93 (MQ=255)
        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  1:5270675/93‑1 (MQ=255)
        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  2:4030822/93‑1 (MQ=255)
        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCcacaca                                                                                 <  1:2110213/93‑1 (MQ=255)
         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                <  2:823514/93‑1 (MQ=255)
         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                >  1:519095/1‑93 (MQ=255)
         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacac                                                                                >  1:2978024/1‑93 (MQ=255)
          aCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCacacacc                                                                               >  2:2123378/1‑92 (MQ=255)
           cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGc                                                                              >  2:1339717/1‑93 (MQ=255)
            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             <  1:1809043/93‑1 (MQ=255)
            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCTGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             >  1:6211567/1‑93 (MQ=255)
            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             >  1:2757842/1‑93 (MQ=255)
            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             <  2:7131153/93‑1 (MQ=255)
            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGcc                                                                             <  1:483685/93‑1 (MQ=255)
             ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCct                                                                            <  1:4239609/93‑1 (MQ=255)
              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           >  1:1183692/1‑93 (MQ=255)
              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           <  2:351546/93‑1 (MQ=255)
              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           <  2:3717363/93‑1 (MQ=255)
              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctg                                                                           >  1:3248223/1‑93 (MQ=255)
               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGCAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          >  1:7637074/1‑93 (MQ=255)
               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          <  2:2407649/93‑1 (MQ=255)
               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgc                                                                          >  2:7665973/1‑93 (MQ=255)
                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCcttctg                                                                        <  2:1176014/93‑1 (MQ=255)
                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  2:3648377/1‑93 (MQ=255)
                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  2:7440562/1‑93 (MQ=255)
                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  1:3411169/1‑93 (MQ=255)
                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCctgctg                                                                        >  2:4568989/1‑93 (MQ=255)
                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  1:7184843/1‑93 (MQ=255)
                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  2:1598903/1‑93 (MQ=255)
                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  2:1718153/1‑93 (MQ=255)
                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTgg                                                                       >  1:4402945/1‑93 (MQ=255)
                   cATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTggg                                                                      >  2:4232307/1‑93 (MQ=255)
                     ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTa                                                                    >  2:1023903/1‑93 (MQ=255)
                      tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                                                                   >  2:4865493/1‑93 (MQ=255)
                      tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                                                                   <  2:4145370/93‑1 (MQ=255)
                      tGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaa                                                                   >  1:6712400/1‑93 (MQ=255)
                       gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGCCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTaaa                                                                  <  2:6613015/93‑1 (MQ=255)
                         cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                                                                >  1:7366814/1‑93 (MQ=255)
                         cGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAaca                                                                >  1:3300217/1‑93 (MQ=255)
                          gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacac                                                               >  2:2118332/1‑93 (MQ=255)
                             cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccac                                                            <  2:6857474/93‑1 (MQ=255)
                              ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTACCACACACGCCTGCTGGGTAAacaccaca                                                           >  2:6955931/1‑93 (MQ=255)
                               tCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAacaccacac                                                          >  1:3142553/1‑93 (MQ=255)
                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       >  1:2912148/1‑93 (MQ=255)
                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       <  1:5609413/93‑1 (MQ=255)
                                  agATCTCAGCCAGCGTC‑GCTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       <  1:6098927/92‑1 (MQ=255)
                                    aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     >  2:3968211/1‑93 (MQ=255)
                                     tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt                                                    >  2:2839402/1‑93 (MQ=255)
                                     tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGt                                                    >  1:7830144/1‑93 (MQ=255)
                                      ctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg                                                   >  1:3466862/1‑93 (MQ=255)
                                          gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               >  1:448261/1‑93 (MQ=255)
                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             <  1:7015212/93‑1 (MQ=255)
                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             >  1:584120/1‑93 (MQ=255)
                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             <  1:6358339/93‑1 (MQ=255)
                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             >  2:5313006/1‑93 (MQ=255)
                                             agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctca                                            >  1:4982342/1‑92 (MQ=255)
                                              gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           <  1:1110960/93‑1 (MQ=255)
                                                 gtcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGata                                         >  2:1962820/4‑93 (MQ=255)
                                                  tcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGatat                                        >  2:5259024/3‑93 (MQ=255)
                                                     ttCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGca                                     <  2:2369651/93‑1 (MQ=255)
                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    >  2:5999814/1‑93 (MQ=255)
                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    <  1:1968922/93‑1 (MQ=255)
                                                      tCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcac                                    >  1:3638377/1‑93 (MQ=255)
                                                        tGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  >  1:4027763/1‑93 (MQ=255)
                                                         ggAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAt                                 >  2:4484031/1‑93 (MQ=255)
                                                          gAAAGCACCGTCAGCGGAGACTGTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATg                                >  2:4978130/1‑93 (MQ=255)
                                                           aaaGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGt                               >  2:5026581/1‑93 (MQ=255)
                                                            aaGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGtt                              <  1:5449998/93‑1 (MQ=255)
                                                             aGCTCCGTCAGCGGAGACTCTTACTCGCCACCTGCATCACACGCCTGCTGGGTAAACCCCACACCATCGTGCGTCTCGATAGGCACAATGTTg                             <  2:4167407/93‑1 (MQ=255)
                                                             aGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTg                             >  1:257522/1‑93 (MQ=255)
                                                             aGCACCGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACCCCATCGTGCGTCTCGATATGCACAATGTTg                             <  2:7567602/93‑1 (MQ=255)
                                                              gCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                            <  2:1420905/93‑1 (MQ=255)
                                                              gCACCGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                            <  1:828210/93‑1 (MQ=255)
                                                                gccGTCAGCGGAGGCTCTTGCTCGCCCCCTGCCACACGCGCCTGCTGGGTAAACACCACACCATCGTCCGTCTCGATATGCAAAATGTTGGTa                          <  2:2910664/92‑1 (MQ=255)
                                                                cccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          <  1:1688394/92‑1 (MQ=255)
                                                                 ccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAt                         <  1:2428515/93‑1 (MQ=255)
                                                                 ccGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAt                         <  2:5615810/93‑1 (MQ=255)
                                                                    tCAGCGGAGCCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      <  2:7067595/93‑1 (MQ=255)
                                                                    tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:1491841/1‑93 (MQ=255)
                                                                    tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:7871285/1‑93 (MQ=255)
                                                                    tCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:7378726/1‑93 (MQ=255)
                                                                      aGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCccg                    <  2:232006/93‑1 (MQ=255)
                                                                          gagaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                >  2:379203/1‑93 (MQ=255)
                                                                          gagaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcga                >  1:7236177/1‑93 (MQ=255)
                                                                           agaCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgac               <  1:1933050/93‑1 (MQ=255)
                                                                             aCTGTTGCCCGCCCCCTGCCACACACGCCTGCTGGGTAAACGCCCCGCAATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga             <  2:6369349/93‑1 (MQ=255)
                                                                              ctctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAt            <  1:2227816/93‑1 (MQ=255)
                                                                              ctctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAt            <  1:7100842/93‑1 (MQ=255)
                                                                               tctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATg           >  1:4656741/1‑93 (MQ=255)
                                                                               tctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTCGGTATGCCCGCCGACGATg           >  1:7697438/1‑93 (MQ=255)
                                                                                ctggCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          <  1:1476314/90‑1 (MQ=255)
                                                                                ctTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          >  1:4645782/1‑93 (MQ=255)
                                                                                   gCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGa       <  2:2606211/93‑1 (MQ=255)
                                                                                       gCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   >  2:3236679/1‑93 (MQ=255)
                                                                                       cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  2:3569316/92‑1 (MQ=255)
                                                                                       cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  1:2011934/92‑1 (MQ=255)
                                                                                       cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGACTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  2:6696038/92‑1 (MQ=255)
                                                                                           |                                                                                         
ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253690‑3253870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC  >  NC_000913/3253687‑3253874
                                                                                              |                                                                                             
CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCC                                                                                               >  SRR4302543.7042088/1‑94 (MQ=60)
CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCC                                                                                               <  SRR4302543.6601763/94‑1 (MQ=60)
CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTGCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCC                                                                                               >  SRR4302543.3905813/1‑94 (MQ=60)
  GATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCT                                                                                             <  SRR4302543.2926904/94‑1 (MQ=60)
   ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTG                                                                                            <  SRR4302543.1434895/94‑1 (MQ=60)
     CGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCC                                                                                          >  SRR4302543.6475169/1‑94 (MQ=60)
        CGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACA                                                                                       >  SRR4302543.657044/1‑94 (MQ=60)
         GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACAC                                                                                      >  SRR4302543.891149/1‑94 (MQ=60)
         GCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACAC                                                                                      <  SRR4302543.7882156/94‑1 (MQ=60)
          CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACA                                                                                     <  SRR4302543.7876862/94‑1 (MQ=60)
           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    <  SRR4302543.5526184/94‑1 (MQ=60)
           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    <  SRR4302543.2129100/94‑1 (MQ=60)
           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    >  SRR4302543.3005067/1‑94 (MQ=60)
           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    <  SRR4302543.309029/94‑1 (MQ=60)
           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    <  SRR4302543.5318223/94‑1 (MQ=60)
           AAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    >  SRR4302543.523915/1‑94 (MQ=60)
            caaagggccctcacttacgcacttcagggtctcacaccgcgtccTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACAC                                                                                    <  SRR4302543.1863394/50‑1 (MQ=60)
            AACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACG                                                                                   >  SRR4302543.7811427/1‑94 (MQ=60)
              CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCTGAGACTCTTGCTCGCCCCCTGCCACACACGCC                                                                                 >  SRR4302543.6270219/1‑94 (MQ=60)
              CGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCC                                                                                 >  SRR4302543.2782927/1‑94 (MQ=60)
               GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCCCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCT                                                                                <  SRR4302543.1825246/94‑1 (MQ=60)
               GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCT                                                                                <  SRR4302543.488154/94‑1 (MQ=60)
               GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCT                                                                                <  SRR4302543.6792218/94‑1 (MQ=60)
               GGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCT                                                                                >  SRR4302543.5592296/1‑94 (MQ=60)
                GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTG                                                                               <  SRR4302543.4278068/94‑1 (MQ=60)
                GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTG                                                                               >  SRR4302543.468382/1‑94 (MQ=60)
                GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTG                                                                               >  SRR4302543.3277589/1‑94 (MQ=60)
                GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTG                                                                               <  SRR4302543.3314730/94‑1 (MQ=60)
                GGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTG                                                                               >  SRR4302543.1194381/1‑94 (MQ=60)
                GGACGTCATTGACGAAGTTCAGGTGCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGGCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTG                                                                               <  SRR4302543.7415445/94‑1 (MQ=60)
                 GACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGCAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGC                                                                              >  SRR4302543.7709530/1‑94 (MQ=60)
                 GACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGC                                                                              >  SRR4302543.6251263/1‑94 (MQ=60)
                 GACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGC                                                                              >  SRR4302543.686704/1‑94 (MQ=60)
                  ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCT                                                                             >  SRR4302543.2382905/1‑94 (MQ=60)
                  ACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCT                                                                             <  SRR4302543.179336/94‑1 (MQ=60)
                   CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTG                                                                            <  SRR4302543.3706079/94‑1 (MQ=60)
                   CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTG                                                                            >  SRR4302543.2310575/1‑94 (MQ=60)
                   CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTG                                                                            >  SRR4302543.5199042/1‑94 (MQ=60)
                   CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTG                                                                            <  SRR4302543.7133947/94‑1 (MQ=60)
                   CTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTG                                                                            >  SRR4302543.3441708/1‑94 (MQ=60)
                    TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGG                                                                           <  SRR4302543.3071369/94‑1 (MQ=60)
                    TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGG                                                                           >  SRR4302543.4442858/1‑94 (MQ=60)
                    TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGG                                                                           <  SRR4302543.2078020/94‑1 (MQ=60)
                    TTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGG                                                                           >  SRR4302543.7254553/1‑94 (MQ=60)
                      CATTGACGATCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGT                                                                         <  SRR4302543.4501234/94‑1 (MQ=60)
                       ATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTA                                                                        >  SRR4302543.7795658/1‑94 (MQ=60)
                       ATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTA                                                                        >  SRR4302543.7459147/1‑94 (MQ=60)
                        TTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAA                                                                       <  SRR4302543.3267670/94‑1 (MQ=60)
                        TTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAA                                                                       >  SRR4302543.6775816/1‑94 (MQ=60)
                        TTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAA                                                                       >  SRR4302543.469480/1‑94 (MQ=60)
                          TACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAAC                                                                     <  SRR4302543.1891406/94‑1 (MQ=60)
                          GACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAAC                                                                     >  SRR4302543.5271609/1‑94 (MQ=60)
                           ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACA                                                                    >  SRR4302543.3329999/1‑94 (MQ=60)
                           ACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACA                                                                    >  SRR4302543.7437648/1‑94 (MQ=60)
                            CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACAC                                                                   <  SRR4302543.5666845/94‑1 (MQ=60)
                            CGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACAC                                                                   <  SRR4302543.4283534/94‑1 (MQ=60)
                               ACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCAC                                                                >  SRR4302543.7268082/1‑94 (MQ=60)
                                 TTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACAC                                                              >  SRR4302543.3170817/1‑94 (MQ=60)
                                    AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                                                           >  SRR4302543.2938615/1‑94 (MQ=60)
                                    AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                                                           >  SRR4302543.5342343/1‑94 (MQ=60)
                                    AGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAT                                                           >  SRR4302543.7192068/1‑94 (MQ=60)
                                     GGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                                                          <  SRR4302543.5660529/94‑1 (MQ=60)
                                     AGATCTCAGCCAGCGTC‑GCTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATC                                                          <  SRR4302543.6156715/94‑1 (MQ=60)
                                       ATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGT                                                        >  SRR4302543.7903877/1‑94 (MQ=60)
                                        TCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTG                                                       >  SRR4302543.3497914/1‑94 (MQ=60)
                                         CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC                                                      <  SRR4302543.217399/94‑1 (MQ=60)
                                         CTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGC                                                      >  SRR4302543.853540/1‑94 (MQ=60)
                                          TCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCG                                                     <  SRR4302543.1313984/94‑1 (MQ=60)
                                          TCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCG                                                     >  SRR4302543.3252042/1‑94 (MQ=60)
                                           CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT                                                    >  SRR4302543.7729131/1‑94 (MQ=60)
                                           CAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT                                                    >  SRR4302543.841102/1‑94 (MQ=60)
                                            AGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTC                                                   >  SRR4302543.452450/1‑94 (MQ=60)
                                              CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC                                                 <  SRR4302543.5475425/94‑1 (MQ=60)
                                              CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC                                                 >  SRR4302543.4730534/1‑94 (MQ=60)
                                              CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC                                                 >  SRR4302543.589451/1‑94 (MQ=60)
                                              CCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTC                                                 <  SRR4302543.2958409/94‑1 (MQ=60)
                                               CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG                                                >  SRR4302543.6199356/1‑94 (MQ=60)
                                               CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG                                                <  SRR4302543.6324446/94‑1 (MQ=60)
                                               CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG                                                <  SRR4302543.6418123/94‑1 (MQ=60)
                                               CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCG                                                <  SRR4302543.7082211/94‑1 (MQ=60)
                                               CAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCA                                                >  SRR4302543.5027698/1‑94 (MQ=60)
                                                AGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGA                                               >  SRR4302543.744909/1‑94 (MQ=60)
                                                 GCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT                                              <  SRR4302543.1120988/94‑1 (MQ=60)
                                                 GCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGAT                                              <  SRR4302543.152763/94‑1 (MQ=60)
                                                  CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA                                             <  SRR4302543.2062350/94‑1 (MQ=60)
                                                  CGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATA                                             >  SRR4302543.3465483/1‑94 (MQ=60)
                                                   GTC‑GTTCTGGAAAGCACCGTCAGCGGTGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT                                            <  SRR4302543.1983402/94‑1 (MQ=60)
                                                   GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT                                            >  SRR4302543.2014059/1‑94 (MQ=60)
                                                   GTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT                                            >  SRR4302543.4227456/1‑94 (MQ=60)
                                                    gtgtTTCTGGAAAGCACCGTCAGCGTAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT                                            <  SRR4302543.1624646/90‑1 (MQ=60)
                                                    gtcgttcgaGAAAGCACCGTCAGCGGAGCCTCTTGCTCCCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATAT                                            <  SRR4302543.1431081/85‑1 (MQ=60)
                                                      CGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGC                                          >  SRR4302543.291377/1‑94 (MQ=60)
                                                        TTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCAC                                        >  SRR4302543.3670398/1‑94 (MQ=60)
                                                        TTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCAC                                        <  SRR4302543.183807/94‑1 (MQ=60)
                                                        TTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCAC                                        <  SRR4302543.6511322/94‑1 (MQ=60)
                                                        TTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCAC                                        >  SRR4302543.4954811/1‑94 (MQ=60)
                                                         TCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACA                                       <  SRR4302543.1986567/94‑1 (MQ=60)
                                                          CTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAA                                      >  SRR4302543.4063761/1‑94 (MQ=60)
                                                           TGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAT                                     <  SRR4302543.7137668/94‑1 (MQ=60)
                                                           TGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAT                                     <  SRR4302543.5844841/94‑1 (MQ=60)
                                                           TGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATCCACAAT                                     <  SRR4302543.7219690/94‑1 (MQ=60)
                                                            GGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATG                                    >  SRR4302543.2930834/1‑94 (MQ=60)
                                                             GAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGT                                   >  SRR4302543.6039589/1‑94 (MQ=60)
                                                             GAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGT                                   <  SRR4302543.929562/94‑1 (MQ=60)
                                                               AAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTG                                 >  SRR4302543.259774/1‑94 (MQ=60)
                                                               AAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTG                                 <  SRR4302543.5499430/94‑1 (MQ=60)
                                                                AGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGG                                >  SRR4302543.1781552/1‑94 (MQ=60)
                                                                AGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGG                                <  SRR4302543.7741599/94‑1 (MQ=60)
                                                                AGCACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGG                                <  SRR4302543.5163157/94‑1 (MQ=60)
                                                                 GCACCGTCAGCGGAGACTCTTGCTCGCCCACTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGT                               <  SRR4302543.835514/94‑1 (MQ=60)
                                                                  CGCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTA                              <  SRR4302543.5935626/94‑1 (MQ=60)
                                                                  CACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTA                              <  SRR4302543.2585275/94‑1 (MQ=60)
                                                                  CACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTA                              <  SRR4302543.7328899/94‑1 (MQ=60)
                                                                   CCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAT                             <  SRR4302543.2024276/94‑1 (MQ=60)
                                                                   CCCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAT                             <  SRR4302543.1703552/94‑1 (MQ=60)
                                                                   ACCGTCAGCGGAGTCTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACCCCATCGTGCGTCTCGATATGCACAATGTTGGTAT                             <  SRR4302543.3520588/94‑1 (MQ=60)
                                                                   ACCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAT                             <  SRR4302543.4285363/94‑1 (MQ=60)
                                                                    CCGTCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATG                            <  SRR4302543.2450431/94‑1 (MQ=60)
                                                                       TCAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCC                         <  SRR4302543.221517/94‑1 (MQ=60)
                                                                        CAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCG                        <  SRR4302543.7377026/94‑1 (MQ=60)
                                                                        CAGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCACCGTGCGTCTCGATATGTACAATGTTGGTATGCCTG                        >  SRR4302543.1091451/1‑94 (MQ=60)
                                                                         AGCGGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGC                       >  SRR4302543.116445/1‑94 (MQ=60)
                                                                         AGCGGAGACTCGTGCTCGCCCCATGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGC                       <  SRR4302543.3413299/94‑1 (MQ=60)
                                                                          GCGAAGACTCTTGCGCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCC                      <  SRR4302543.3597811/94‑1 (MQ=60)
                                                                            GGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGC                    >  SRR4302543.7983442/1‑94 (MQ=60)
                                                                            GGAGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGA                    >  SRR4302543.7306242/1‑94 (MQ=60)
                                                                              AGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACG                  <  SRR4302543.1950434/94‑1 (MQ=60)
                                                                              AGACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACG                  >  SRR4302543.4985084/1‑94 (MQ=60)
                                                                                ACTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAT                <  SRR4302543.4610544/94‑1 (MQ=60)
                                                                                 CTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG               <  SRR4302543.7169804/94‑1 (MQ=60)
                                                                                 CTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG               <  SRR4302543.2247933/94‑1 (MQ=60)
                                                                                 CTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG               >  SRR4302543.4699151/1‑94 (MQ=60)
                                                                                 CTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATG               >  SRR4302543.4699150/1‑94 (MQ=60)
                                                                                 CTCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTCGGTATGCCCGCCGACGATG               >  SRR4302543.7770301/1‑94 (MQ=60)
                                                                                  TCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGG              >  SRR4302543.4688053/1‑94 (MQ=60)
                                                                                  TCTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGG              <  SRR4302543.3941110/94‑1 (MQ=60)
                                                                                   CTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGT             <  SRR4302543.4789825/94‑1 (MQ=60)
                                                                                   CTTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGT             <  SRR4302543.2483878/94‑1 (MQ=60)
                                                                                   CTGGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGT             <  SRR4302543.1489619/94‑1 (MQ=60)
                                                                                    TTGCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTG            <  SRR4302543.3299400/94‑1 (MQ=60)
                                                                                      GCTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGAC          <  SRR4302543.3490372/94‑1 (MQ=60)
                                                                                       CTCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACA         >  SRR4302543.2608682/1‑94 (MQ=60)
                                                                                        TCGCCCCCTGCCACACACGCCTGCTGGGTAAACCCCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACAC        <  SRR4302543.6614930/94‑1 (MQ=60)
                                                                                        TCGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACAC        <  SRR4302543.6614931/94‑1 (MQ=60)
                                                                                         CGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACA       <  SRR4302543.4226962/94‑1 (MQ=60)
                                                                                         CGCCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACA       >  SRR4302543.99057/1‑94 (MQ=60)
                                                                                           CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACG     <  SRR4302543.7903862/94‑1 (MQ=60)
                                                                                            CCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGC    >  SRR4302543.2591292/1‑94 (MQ=60)
                                                                                            CCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGC    >  SRR4302543.2376200/1‑94 (MQ=60)
                                                                                             CCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCC   <  SRR4302543.4225125/94‑1 (MQ=60)
                                                                                             CCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGGGACACACGCC   >  SRR4302543.6469119/1‑94 (MQ=60)
                                                                                              CCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC  >  SRR4302543.3360842/1‑94 (MQ=60)
                                                                                              CCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC  >  SRR4302543.3221687/1‑94 (MQ=60)
                                                                                              CCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC  >  SRR4302543.3018581/1‑94 (MQ=60)
                                                                                              CCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC  >  SRR4302543.5124071/1‑94 (MQ=60)
                                                                                              |                                                                                             
CGGATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACACGCCC  >  NC_000913/3253687‑3253874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑