Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,340,190 A→G T93A (ACT→GCT)  yrbI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,340,1900AG99.7% 1049.6 / ‑5.8 332T93A (ACT→GCT) yrbIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (158/173);  total (159/173)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 348 total aligned reads displayed.

GTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340101‑3340279
                                                                                         |                                                                                         
gTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctc                                                                                        >  1:5434602/1‑93 (MQ=255)
 ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctca                                                                                       <  2:6717820/93‑1 (MQ=255)
 ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctca                                                                                       <  2:7159744/93‑1 (MQ=255)
    tgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACtt                                                                                    <  1:1661744/93‑1 (MQ=255)
       gcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTa                                                                                 <  1:4634657/93‑1 (MQ=255)
        cTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAt                                                                                >  2:1993863/1‑93 (MQ=255)
        cTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAt                                                                                <  1:5737165/93‑1 (MQ=255)
         tCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATc                                                                               <  1:7199507/93‑1 (MQ=255)
          cACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCa                                                                              >  1:1865360/1‑93 (MQ=255)
           aCCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAg                                                                             >  1:6245644/1‑93 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  1:3947639/93‑1 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            >  1:546402/1‑93 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  2:5402080/93‑1 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  2:5163220/93‑1 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  2:4022607/93‑1 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  2:3922836/93‑1 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            >  1:4736314/1‑93 (MQ=255)
            ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  2:3241662/93‑1 (MQ=255)
            ccTCTGATATTGAAGTAGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            <  2:6999547/93‑1 (MQ=255)
             ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           <  1:3610298/93‑1 (MQ=255)
              tctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          >  1:407005/1‑93 (MQ=255)
              tctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          >  1:4298037/1‑93 (MQ=255)
                tGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCa                                                                        <  2:7887891/93‑1 (MQ=255)
                 gATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAg                                                                       >  2:2057113/1‑93 (MQ=255)
                 gATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAg                                                                       >  2:2603235/1‑93 (MQ=255)
                  atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      >  2:6128844/1‑93 (MQ=255)
                  atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      >  2:933753/1‑93 (MQ=255)
                   tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     >  2:2627927/1‑93 (MQ=255)
                   tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     <  2:7913639/93‑1 (MQ=255)
                   tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     >  1:1421090/1‑93 (MQ=255)
                   tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     >  2:1816153/1‑93 (MQ=255)
                     ttGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaa                                                                   >  2:6330507/1‑93 (MQ=255)
                     ttGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaa                                                                   >  2:1221840/1‑93 (MQ=255)
                     ttGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaa                                                                   >  2:4761391/1‑93 (MQ=255)
                       gAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaac                                                                 <  2:3141754/93‑1 (MQ=255)
                        aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                >  1:748399/1‑93 (MQ=255)
                        aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                <  2:84134/93‑1 (MQ=255)
                         aGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaacaa                                                               <  1:3361043/93‑1 (MQ=255)
                          gTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGAGCCACATTGGGGATCGCTCACGTGTATCAGGGGGAGTcaaacaaa                                                              >  1:2655695/1‑93 (MQ=255)
                            cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  1:6023270/93‑1 (MQ=255)
                            cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  1:5858203/93‑1 (MQ=255)
                            cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  2:576944/93‑1 (MQ=255)
                            cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  1:2134932/93‑1 (MQ=255)
                             gCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTg                                                           <  2:6215374/93‑1 (MQ=255)
                               tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:4233955/93‑1 (MQ=255)
                               tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:4019296/93‑1 (MQ=255)
                               tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  2:5947744/93‑1 (MQ=255)
                               tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         >  2:5509962/1‑93 (MQ=255)
                                aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        >  2:3641986/1‑93 (MQ=255)
                                 tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  1:7755748/1‑93 (MQ=255)
                                 tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  2:3711554/1‑93 (MQ=255)
                                 tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  2:3540339/1‑93 (MQ=255)
                                  cATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGc                                                      >  2:7437012/1‑93 (MQ=255)
                                    ttACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCt                                                    >  1:2842253/1‑93 (MQ=255)
                                        cGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAATCTGATCGCCTTTAg                                                <  2:464987/93‑1 (MQ=255)
                                        cGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAg                                                <  1:3929405/93‑1 (MQ=255)
                                        cGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATAGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAg                                                <  1:3244414/93‑1 (MQ=255)
                                         gggCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGc                                               <  1:434051/93‑1 (MQ=255)
                                         gggCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGc                                               <  2:274811/93‑1 (MQ=255)
                                          ggCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCg                                              >  2:7611743/1‑93 (MQ=255)
                                          ggCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCg                                              >  1:2476594/1‑93 (MQ=255)
                                          ggCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCg                                              >  2:3160159/1‑93 (MQ=255)
                                           gCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGa                                             <  2:1180105/93‑1 (MQ=255)
                                            cGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAt                                            <  1:1290804/93‑1 (MQ=255)
                                            cGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAt                                            <  2:1565619/93‑1 (MQ=255)
                                            cGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAt                                            <  1:1000119/93‑1 (MQ=255)
                                            cGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAt                                            <  1:4898691/93‑1 (MQ=255)
                                             gAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATc                                           >  1:6604707/1‑93 (MQ=255)
                                             gAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATc                                           <  1:2877527/93‑1 (MQ=255)
                                                aGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgc                                        <  2:7544527/93‑1 (MQ=255)
                                                 ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATcggct                                       <  1:1158249/93‑1 (MQ=255)
                                                  gCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgctg                                      <  1:873378/93‑1 (MQ=255)
                                                    tAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGa                                    <  1:1894892/93‑1 (MQ=255)
                                                     aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   >  2:4078994/1‑93 (MQ=255)
                                                       aCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaaa                                 >  2:4473087/1‑93 (MQ=255)
                                                         tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               <  1:1772658/93‑1 (MQ=255)
                                                           gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             <  1:5340081/93‑1 (MQ=255)
                                                            tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            >  2:5683137/1‑93 (MQ=255)
                                                            tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  2:7364097/93‑1 (MQ=255)
                                                            tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  2:2796910/93‑1 (MQ=255)
                                                            gagaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  1:6867778/92‑1 (MQ=255)
                                                             agaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGc                           <  1:7843997/93‑1 (MQ=255)
                                                                agaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAt                        >  2:2482430/1‑93 (MQ=255)
                                                                   tCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGc                     >  1:5593534/1‑93 (MQ=255)
                                                                   tCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGc                     >  1:121309/1‑93 (MQ=255)
                                                                   tCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGc                     >  2:5023329/1‑93 (MQ=255)
                                                                   tCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGc                     >  2:6867410/1‑93 (MQ=255)
                                                                     gTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGccc                   <  2:232265/93‑1 (MQ=255)
                                                                     gTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGccc                   <  1:3427038/93‑1 (MQ=255)
                                                                      ttGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGcccc                  >  2:44036/1‑93 (MQ=255)
                                                                        gtgCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCgg                >  1:2275088/1‑93 (MQ=255)
                                                                              caTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtg          >  1:5181286/1‑93 (MQ=255)
                                                                               aTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgt         >  1:6362029/1‑93 (MQ=255)
                                                                               aTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgt         <  1:7599078/93‑1 (MQ=255)
                                                                                ttGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgtg        >  2:4323175/1‑93 (MQ=255)
                                                                                    ggATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCtt    >  1:6072844/1‑93 (MQ=255)
                                                                                     gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  2:3809642/93‑1 (MQ=255)
                                                                                     gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  2:1665175/93‑1 (MQ=255)
                                                                                      aTCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGTAAATGTGGCTTAt  >  1:3003982/1‑93 (MQ=255)
                                                                                      aTCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAt  <  1:3760809/93‑1 (MQ=255)
                                                                                         |                                                                                         
GTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340101‑3340279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  >  NC_000913/3340097‑3340283
                                                                                             |                                                                                             
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302543.1952259/1‑94 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               <  SRR4302543.4012762/94‑1 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               <  SRR4302543.7255995/94‑1 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302543.3918267/1‑94 (MQ=60)
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCG                                                                                               >  SRR4302543.695449/1‑94 (MQ=60)
 TTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGC                                                                                              >  SRR4302543.3170619/1‑94 (MQ=60)
  TCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCT                                                                                             <  SRR4302543.931138/94‑1 (MQ=60)
  TCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCT                                                                                             <  SRR4302543.7483377/94‑1 (MQ=60)
   CGTTGTGCGCTCACCTCTGATATTGAAGTTGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTC                                                                                            >  SRR4302543.715125/1‑94 (MQ=60)
   CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTC                                                                                            >  SRR4302543.5483967/1‑94 (MQ=60)
    GTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCA                                                                                           >  SRR4302543.63686/1‑94 (MQ=60)
     TTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCAC                                                                                          <  SRR4302543.3357384/94‑1 (MQ=60)
     TTGTGCGCTCACCTCTGATATTGAAGTCGCTATAATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCAC                                                                                          >  SRR4302543.6170535/1‑94 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTG                                                                                       <  SRR4302543.1676630/94‑1 (MQ=60)
        TGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTG                                                                                       <  SRR4302543.3022971/94‑1 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAT                                                                                    >  SRR4302543.713254/1‑94 (MQ=60)
           GCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTAT                                                                                    <  SRR4302543.4676794/94‑1 (MQ=60)
            CTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   >  SRR4302543.3686142/1‑94 (MQ=60)
            CTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   <  SRR4302543.5789487/94‑1 (MQ=60)
            CTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATC                                                                                   <  SRR4302543.1290818/94‑1 (MQ=60)
             TCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCA                                                                                  <  SRR4302543.7269311/94‑1 (MQ=60)
             TCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCA                                                                                  >  SRR4302543.1882114/1‑94 (MQ=60)
              CACCTCTGATATTGAGGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 >  SRR4302543.7955169/1‑94 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302543.2509673/94‑1 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302543.4152013/94‑1 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 <  SRR4302543.5146326/94‑1 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 >  SRR4302543.5485377/1‑94 (MQ=60)
              CACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAG                                                                                 >  SRR4302543.6304567/1‑94 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302543.4779601/1‑94 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302543.551442/1‑94 (MQ=60)
               ACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGG                                                                                >  SRR4302543.551443/1‑94 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               <  SRR4302543.5492791/94‑1 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               <  SRR4302543.3982537/94‑1 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               <  SRR4302543.3627502/94‑1 (MQ=60)
                CCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGG                                                                               <  SRR4302543.7613612/94‑1 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              >  SRR4302543.4337095/1‑94 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              >  SRR4302543.4296530/1‑94 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              <  SRR4302543.3642141/94‑1 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              >  SRR4302543.6926495/1‑94 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              <  SRR4302543.728001/94‑1 (MQ=60)
                 CTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGG                                                                              >  SRR4302543.410759/1‑94 (MQ=60)
                   CTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCA                                                                            >  SRR4302543.1131642/1‑94 (MQ=60)
                   CTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATAGCTCACTTGTATCAGGGGCA                                                                            >  SRR4302543.5634551/1‑94 (MQ=60)
                    TGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAG                                                                           >  SRR4302543.2271252/1‑94 (MQ=60)
                    TGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAG                                                                           <  SRR4302543.215848/94‑1 (MQ=60)
                    TGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAG                                                                           >  SRR4302543.6004850/1‑94 (MQ=60)
                     GATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGT                                                                          >  SRR4302543.7666518/1‑94 (MQ=60)
                      ATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTC                                                                         >  SRR4302543.1433954/1‑94 (MQ=60)
                       TATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCA                                                                        <  SRR4302543.4071095/94‑1 (MQ=60)
                       TATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCA                                                                        >  SRR4302543.2338724/1‑94 (MQ=60)
                        ATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAA                                                                       <  SRR4302543.3571727/94‑1 (MQ=60)
                          TGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAAC                                                                     <  SRR4302543.3394847/94‑1 (MQ=60)
                          TGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAAC                                                                     >  SRR4302543.7107799/1‑94 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    <  SRR4302543.3747328/94‑1 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    <  SRR4302543.300922/94‑1 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    <  SRR4302543.2709938/94‑1 (MQ=60)
                           GAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACA                                                                    >  SRR4302543.755048/1‑94 (MQ=60)
                            AAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAA                                                                   >  SRR4302543.1537252/1‑94 (MQ=60)
                             AGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAA                                                                  <  SRR4302543.3391271/94‑1 (MQ=60)
                             AGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGAGCCACATTGGGGATCGCTCACGTGTATCAGGGGGAGTCAAACAAA                                                                  >  SRR4302543.2679773/1‑94 (MQ=60)
                              GTCGCTATCATTACCGGGCGAAAGGCTAACCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAAC                                                                 <  SRR4302543.424776/94‑1 (MQ=60)
                              GTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAAC                                                                 >  SRR4302543.6391299/1‑94 (MQ=60)
                               TCGCTATCATTACCGGGCGAAAGGCTAAACTGGAAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACT                                                                <  SRR4302543.7685483/94‑1 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               >  SRR4302543.617204/1‑94 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               <  SRR4302543.6079236/94‑1 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               <  SRR4302543.2154036/94‑1 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               <  SRR4302543.5911531/94‑1 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               >  SRR4302543.7809036/1‑94 (MQ=60)
                                CGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTG                                                               <  SRR4302543.6079235/94‑1 (MQ=60)
                                 GCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGA                                                              <  SRR4302543.5829818/94‑1 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAT                                                             <  SRR4302543.41677/94‑1 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAT                                                             <  SRR4302543.1395779/94‑1 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAT                                                             >  SRR4302543.3372031/1‑94 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACGTGTATCAGGGGCAGTCCAACAAACTGAT                                                             >  SRR4302543.1665047/1‑94 (MQ=60)
                                  CTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCCTACTTGTATCAGGGGCAGTCAAACAAACTGAG                                                             >  SRR4302543.4048495/1‑94 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302543.4877060/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302543.4272362/94‑1 (MQ=60)
                                   TATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATC                                                            <  SRR4302543.4055191/94‑1 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           <  SRR4302543.5743404/94‑1 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           >  SRR4302543.7828959/1‑94 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           <  SRR4302543.1197536/94‑1 (MQ=60)
                                    ATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCG                                                           <  SRR4302543.1767378/94‑1 (MQ=60)
                                     TCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGC                                                          >  SRR4302543.487244/1‑94 (MQ=60)
                                     TCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGC                                                          >  SRR4302543.4600866/1‑94 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        >  SRR4302543.2868224/1‑94 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        >  SRR4302543.3316972/1‑94 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        <  SRR4302543.3976775/94‑1 (MQ=60)
                                       ATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCT                                                        >  SRR4302543.1796809/1‑94 (MQ=60)
                                         TACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTT                                                      <  SRR4302543.1192346/94‑1 (MQ=60)
                                         TACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTT                                                      >  SRR4302543.2366318/1‑94 (MQ=60)
                                          ACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTA                                                     <  SRR4302543.1235120/94‑1 (MQ=60)
                                           CCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAG                                                    <  SRR4302543.3074502/94‑1 (MQ=60)
                                           CCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAG                                                    <  SRR4302543.1905163/94‑1 (MQ=60)
                                           CCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAG                                                    >  SRR4302543.4346709/1‑94 (MQ=60)
                                            CGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGC                                                   <  SRR4302543.3964093/94‑1 (MQ=60)
                                            CGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTACCAGGGGCAGTCAAACAAACTGATCGCCTTTAGC                                                   <  SRR4302543.399753/94‑1 (MQ=60)
                                            CGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATAGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGC                                                   <  SRR4302543.3273754/94‑1 (MQ=60)
                                             GGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCG                                                  >  SRR4302543.2498991/1‑94 (MQ=60)
                                             GGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCG                                                  <  SRR4302543.5417713/94‑1 (MQ=60)
                                             GGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCG                                                  >  SRR4302543.7887135/1‑94 (MQ=60)
                                             GGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCG                                                  <  SRR4302543.438120/94‑1 (MQ=60)
                                              GGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGA                                                 >  SRR4302543.1908175/1‑94 (MQ=60)
                                               GCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAT                                                >  SRR4302543.2898824/1‑94 (MQ=60)
                                               GCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAT                                                <  SRR4302543.2934961/94‑1 (MQ=60)
                                               GCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGAT                                                >  SRR4302543.4758755/1‑94 (MQ=60)
                                               GCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCGTTAGCGAT                                                >  SRR4302543.4758754/1‑94 (MQ=60)
                                                CGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATC                                               <  SRR4302543.1008950/94‑1 (MQ=60)
                                                CGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATC                                               <  SRR4302543.5952074/94‑1 (MQ=60)
                                                CGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATC                                               <  SRR4302543.1302296/94‑1 (MQ=60)
                                                CGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATC                                               >  SRR4302543.6666956/1‑94 (MQ=60)
                                                CGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATC                                               <  SRR4302543.4943343/94‑1 (MQ=60)
                                                 GAAAGGCTCAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCCGGGACAGTCAAACAAACTGATCGCCTTTAGCGATCT                                              >  SRR4302543.5779981/1‑94 (MQ=60)
                                                 GAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCT                                              <  SRR4302543.2903712/94‑1 (MQ=60)
                                                    AGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCT                                           >  SRR4302543.5782020/1‑94 (MQ=60)
                                                     GGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTG                                          >  SRR4302543.4922714/1‑94 (MQ=60)
                                                     GGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTG                                          <  SRR4302543.153220/94‑1 (MQ=60)
                                                     GGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCGGCTG                                          <  SRR4302543.1168794/94‑1 (MQ=60)
                                                      GCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGG                                         <  SRR4302543.881158/94‑1 (MQ=60)
                                                      GCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGG                                         <  SRR4302543.6835918/94‑1 (MQ=60)
                                                       CTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGA                                        >  SRR4302543.386847/1‑94 (MQ=60)
                                                        TAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAA                                       <  SRR4302543.1911931/94‑1 (MQ=60)
                                                         AAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAA                                      <  SRR4302543.6288217/94‑1 (MQ=60)
                                                           ACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAA                                    >  SRR4302543.1852196/1‑94 (MQ=60)
                                                            CTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAACC                                   >  SRR4302543.2652513/1‑94 (MQ=60)
                                                            CTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAC                                   >  SRR4302543.3946547/1‑94 (MQ=60)
                                                            CTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAC                                   >  SRR4302543.4522576/1‑94 (MQ=60)
                                                            CTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAC                                   <  SRR4302543.4295496/94‑1 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  <  SRR4302543.3978225/94‑1 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  <  SRR4302543.1788503/94‑1 (MQ=60)
                                                             TGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACT                                  <  SRR4302543.1788502/94‑1 (MQ=60)
                                                              GGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTG                                 <  SRR4302543.6103711/94‑1 (MQ=60)
                                                              GGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTG                                 <  SRR4302543.4338300/94‑1 (MQ=60)
                                                               GTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGG                                >  SRR4302543.2868528/1‑94 (MQ=60)
                                                               GTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGG                                <  SRR4302543.5388387/94‑1 (MQ=60)
                                                               GTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGG                                >  SRR4302543.3627486/1‑94 (MQ=60)
                                                               GTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGG                                >  SRR4302543.131734/1‑94 (MQ=60)
                                                               GTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGG                                >  SRR4302543.5383365/1‑94 (MQ=60)
                                                                TAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGC                               <  SRR4302543.4488789/94‑1 (MQ=60)
                                                                GAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGC                               <  SRR4302543.6932468/94‑1 (MQ=60)
                                                                 AGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              >  SRR4302543.3813707/1‑94 (MQ=60)
                                                                 AGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              >  SRR4302543.5357850/1‑94 (MQ=60)
                                                                 AGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              >  SRR4302543.286453/1‑94 (MQ=60)
                                                                 AGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCG                              <  SRR4302543.7917930/94‑1 (MQ=60)
                                                                   AAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAT                            >  SRR4302543.5417316/1‑94 (MQ=60)
                                                                    AGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATT                           >  SRR4302543.341595/1‑94 (MQ=60)
                                                                      ATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGC                         >  SRR4302543.7873937/1‑94 (MQ=60)
                                                                      ATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGC                         >  SRR4302543.122405/1‑94 (MQ=60)
                                                                      ATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGC                         >  SRR4302543.5644483/1‑94 (MQ=60)
                                                                       TCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCC                        <  SRR4302543.6537510/94‑1 (MQ=60)
                                                                        CGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCC                       >  SRR4302543.5132363/1‑94 (MQ=60)
                                                                        CGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCC                       >  SRR4302543.201229/1‑94 (MQ=60)
                                                                         GTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCC                      <  SRR4302543.3457703/94‑1 (MQ=60)
                                                                          TTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCG                     <  SRR4302543.4022763/94‑1 (MQ=60)
                                                                          TTGTGCCACATTGGGAATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCG                     <  SRR4302543.192640/94‑1 (MQ=60)
                                                                           TGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGG                    >  SRR4302543.2295892/1‑94 (MQ=60)
                                                                           TGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGG                    >  SRR4302543.5569816/1‑94 (MQ=60)
                                                                                CACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAT               <  SRR4302543.1120065/94‑1 (MQ=60)
                                                                                CACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAT               <  SRR4302543.6554195/94‑1 (MQ=60)
                                                                                CACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAT               >  SRR4302543.5359162/1‑94 (MQ=60)
                                                                                 ACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATG              >  SRR4302543.5228048/1‑94 (MQ=60)
                                                                                  CATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGT             >  SRR4302543.6421856/1‑94 (MQ=60)
                                                                                  CATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGT             >  SRR4302543.2008203/1‑94 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            <  SRR4302543.7671247/94‑1 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            <  SRR4302543.7671246/94‑1 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            <  SRR4302543.2690561/94‑1 (MQ=60)
                                                                                   ATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTG            >  SRR4302543.735122/1‑94 (MQ=60)
                                                                                    TTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGG           <  SRR4302543.5809793/94‑1 (MQ=60)
                                                                                    TTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGG           <  SRR4302543.7927016/94‑1 (MQ=60)
                                                                                      GGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCT         >  SRR4302543.407576/1‑94 (MQ=60)
                                                                                       GGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTT        >  SRR4302543.7831723/1‑94 (MQ=60)
                                                                                       GGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTT        >  SRR4302543.6087698/1‑94 (MQ=60)
                                                                                       GGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTT        >  SRR4302543.6130400/1‑94 (MQ=60)
                                                                                        GGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTA       >  SRR4302543.2450712/1‑94 (MQ=60)
                                                                                        GGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTA       >  SRR4302543.1837329/1‑94 (MQ=60)
                                                                                         GATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGTAAATGTGGCTTAT      >  SRR4302543.3031208/1‑94 (MQ=60)
                                                                                         GATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT      <  SRR4302543.1230855/94‑1 (MQ=60)
                                                                                         GATCGATCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT      >  SRR4302543.7911221/1‑94 (MQ=60)
                                                                                          ATCTCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATG     <  SRR4302543.6509757/94‑1 (MQ=60)
                                                                                          ATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATG     >  SRR4302543.2576241/1‑94 (MQ=60)
                                                                                          ATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATG     >  SRR4302543.6568959/1‑94 (MQ=60)
                                                                                          ATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATG     <  SRR4302543.3130033/94‑1 (MQ=60)
                                                                                          ATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATG     <  SRR4302543.3793906/94‑1 (MQ=60)
                                                                                            CGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTC   <  SRR4302543.2270593/94‑1 (MQ=60)
                                                                                            CGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTC   >  SRR4302543.2076215/1‑94 (MQ=60)
                                                                                            CGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTC   <  SRR4302543.4055171/94‑1 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  <  SRR4302543.358447/94‑1 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  <  SRR4302543.1844677/94‑1 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  <  SRR4302543.2362928/94‑1 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  <  SRR4302543.5200600/94‑1 (MQ=60)
                                                                                             GCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTCAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  >  SRR4302543.3028348/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTCGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTATGTCG  >  NC_000913/3340097‑3340283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑