Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,497,141 A→G A19A (GCA→GCG)  yhfL → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,497,1410AG99.0% 642.3 / ‑3.6 205A19A (GCA→GCG) yhfLhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (178/25);  total (180/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 211 total aligned reads displayed.

TATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGG  >  NC_000913/3497052‑3497230
                                                                                         |                                                                                         
tatTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCgtgt                                                                                        >  1:2146614/1‑93 (MQ=255)
tatTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCgtgt                                                                                        <  2:6870886/93‑1 (MQ=255)
 attaattaATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTa                                                                                       <  1:2559415/93‑1 (MQ=255)
  ttaattaaTATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTAc                                                                                      >  1:900409/1‑93 (MQ=255)
    aattaatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTg                                                                                    >  1:5608571/1‑93 (MQ=255)
    aattaatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTg                                                                                    >  2:611546/1‑93 (MQ=255)
      ttaatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGt                                                                                  >  1:281207/1‑93 (MQ=255)
        aatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCa                                                                                >  2:6816357/1‑93 (MQ=255)
        aatATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCa                                                                                >  1:6530645/1‑93 (MQ=255)
          tatataATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCatt                                                                              >  2:6009876/1‑92 (MQ=255)
           atataATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCatat                                                                             >  2:2298598/1‑93 (MQ=255)
            tataATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATAtt                                                                            >  1:1640344/1‑93 (MQ=255)
            tataATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGGACTGGTCATAtt                                                                            >  1:2931479/1‑93 (MQ=255)
            tataATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGGACTGGTCATAtt                                                                            >  2:7047244/1‑93 (MQ=255)
             ataataCATAGGGAATGTAAATGAACTAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTg                                                                           >  1:396351/1‑93 (MQ=255)
             ataataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTg                                                                           >  1:6626620/1‑93 (MQ=255)
              taataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGa                                                                          >  1:2463997/1‑93 (MQ=255)
              taataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGa                                                                          >  2:3508757/1‑93 (MQ=255)
               aataCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCGACGTTAACTGCGTGTACTGGTCATATTGaa                                                                         >  1:3251800/1‑93 (MQ=255)
                 taCATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGaaaa                                                                       <  1:5129244/93‑1 (MQ=255)
                   cATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAAcc                                                                     >  2:2524112/1‑93 (MQ=255)
                     tAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGt                                                                   >  1:2393594/1‑93 (MQ=255)
                     tAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGCACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGt                                                                   <  2:6257702/93‑1 (MQ=255)
                      aGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTg                                                                  >  2:7478323/1‑93 (MQ=255)
                       gggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGa                                                                 >  1:818015/1‑93 (MQ=255)
                       gggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGa                                                                 >  2:524434/1‑93 (MQ=255)
                        ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGAt                                                                >  1:427762/1‑93 (MQ=255)
                        ggAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGAt                                                                <  1:1525519/93‑1 (MQ=255)
                         gAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATa                                                               >  1:5448810/1‑93 (MQ=255)
                          aaTGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATaa                                                              <  1:6827051/93‑1 (MQ=255)
                          aaTGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATaa                                                              >  2:3943239/1‑93 (MQ=255)
                           aTGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAg                                                             >  2:3233302/1‑93 (MQ=255)
                            tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGa                                                            >  1:3395187/1‑93 (MQ=255)
                            tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGa                                                            >  2:3293075/1‑93 (MQ=255)
                            tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGa                                                            >  2:5726780/1‑93 (MQ=255)
                            tGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGa                                                            >  2:7731585/1‑93 (MQ=255)
                             gTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGaa                                                           <  1:3872614/93‑1 (MQ=255)
                             gTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGGGCCGTACTGGGTACGTTAACTGCGTGGACGGGGCATATTGAAAACCGTGATAAGaa                                                           >  1:3091622/1‑93 (MQ=255)
                              tAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAAc                                                          >  1:7487026/1‑93 (MQ=255)
                              tAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAAc                                                          >  2:420920/1‑93 (MQ=255)
                              tAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAAc                                                          >  1:2589056/1‑93 (MQ=255)
                               aaaTGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACt                                                         >  2:3586990/1‑93 (MQ=255)
                                 aTGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGc                                                       >  1:6236638/1‑93 (MQ=255)
                                  tGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGct                                                      >  2:2158234/1‑93 (MQ=255)
                                    aaCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGctct                                                    >  1:7187742/1‑93 (MQ=255)
                                    aaCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGctct                                                    >  2:4752544/1‑93 (MQ=255)
                                     aCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCtt                                                   <  2:7870034/93‑1 (MQ=255)
                                     aCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCtt                                                   >  1:232448/1‑93 (MQ=255)
                                     aCAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCtt                                                   >  1:6161922/1‑93 (MQ=255)
                                      cAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTa                                                  >  2:7359863/1‑93 (MQ=255)
                                       aaaTTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTAc                                                 >  2:2957421/1‑93 (MQ=255)
                                       aaaTTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTAc                                                 >  2:2343828/1‑93 (MQ=255)
                                        aaTTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACg                                                >  2:2886402/1‑93 (MQ=255)
                                        aaTTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACg                                                >  1:4339756/1‑93 (MQ=255)
                                        aaTTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACg                                                >  2:1409291/1‑93 (MQ=255)
                                          tttATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTAAGAc                                              >  1:364862/1‑93 (MQ=255)
                                           ttattaAAGTTGCACTGGTAGGGGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACt                                             >  1:3091840/1‑93 (MQ=255)
                                              ttaAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTAcc                                          >  1:6699365/1‑93 (MQ=255)
                                                 aaGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACctgc                                       >  1:3017620/1‑93 (MQ=255)
                                                  aGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACctgct                                      >  2:2363730/1‑93 (MQ=255)
                                                   gTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACctgctg                                     >  2:5784216/1‑93 (MQ=255)
                                                    ttGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCtgctgc                                    >  2:4879536/1‑93 (MQ=255)
                                                      gCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAc                                  >  2:187497/1‑93 (MQ=255)
                                                      gCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAc                                  <  1:7348082/93‑1 (MQ=255)
                                                      gCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAc                                  >  1:7634516/1‑93 (MQ=255)
                                                       cACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAcc                                 <  2:3773126/93‑1 (MQ=255)
                                                        aCTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAccc                                >  2:4871657/1‑93 (MQ=255)
                                                        aCTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAccc                                >  2:2437336/1‑93 (MQ=255)
                                                          tGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCgg                              >  2:4004339/1‑93 (MQ=255)
                                                          tGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCgg                              >  2:5047246/1‑93 (MQ=255)
                                                           ggTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGc                             >  2:2811787/1‑93 (MQ=255)
                                                             tAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCaa                           <  1:4875584/93‑1 (MQ=255)
                                                               ggTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatt                         >  1:3820801/1‑93 (MQ=255)
                                                               ggTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatt                         >  2:5651878/1‑93 (MQ=255)
                                                               ggTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatt                         >  1:1157009/1‑93 (MQ=255)
                                                                 tGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttc                       >  2:4582642/1‑93 (MQ=255)
                                                                  gCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttct                      >  1:3970129/1‑93 (MQ=255)
                                                                   cAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttcta                     >  1:1469820/1‑93 (MQ=255)
                                                                    aGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctat                    >  1:2202565/1‑93 (MQ=255)
                                                                     gTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatt                   >  1:1747474/1‑93 (MQ=255)
                                                                     gTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatt                   >  1:1025997/1‑93 (MQ=255)
                                                                     gTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatt                   >  1:3676512/1‑93 (MQ=255)
                                                                     gTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatt                   >  1:7589313/1‑93 (MQ=255)
                                                                     gTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatt                   >  2:1451182/1‑93 (MQ=255)
                                                                      tACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctattt                  >  1:4107147/1‑93 (MQ=255)
                                                                       aCTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatttc                 >  2:1193225/1‑93 (MQ=255)
                                                                        cTGGCTACGTTAACTGCATGTACTGGTCATATTGAACACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAatttctatttct                >  2:7546667/1‑93 (MQ=255)
                                                                         tGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAAtttctatttcta               >  1:3561623/1‑93 (MQ=255)
                                                                         tGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAAtttctatttcta               >  2:7075545/1‑93 (MQ=255)
                                                                          ggCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTaa              >  2:5158166/1‑93 (MQ=255)
                                                                          ggCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTaa              >  1:7496278/1‑93 (MQ=255)
                                                                          ggCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTaa              >  2:6430133/1‑93 (MQ=255)
                                                                          ggCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTaa              >  1:4470659/1‑93 (MQ=255)
                                                                            cTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTaaaa            >  2:6545964/1‑93 (MQ=255)
                                                                             tACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAAt           >  1:7406943/1‑93 (MQ=255)
                                                                                 ttAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCAtt       >  2:1732761/1‑93 (MQ=255)
                                                                                    aCTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGc    >  1:5017289/1‑93 (MQ=255)
                                                                                    aCTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGc    <  2:5999913/93‑1 (MQ=255)
                                                                                      tGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCgg  >  2:2415096/1‑93 (MQ=255)
                                                                                      tGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCgg  >  1:5155471/1‑93 (MQ=255)
                                                                                         |                                                                                         
TATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGG  >  NC_000913/3497052‑3497230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGC  >  NC_000913/3497051‑3497234
                                                                                          |                                                                                             
ATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGT                                                                                            >  SRR4302543.2165832/1‑94 (MQ=60)
  ATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTACCTGCGTGTAC                                                                                          <  SRR4302543.7868831/94‑1 (MQ=60)
  ATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTAC                                                                                          >  SRR4302543.908356/1‑94 (MQ=60)
  ATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTAC                                                                                          <  SRR4302543.2582802/94‑1 (MQ=60)
  ATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTAC                                                                                          <  SRR4302543.2582803/94‑1 (MQ=60)
    TAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTG                                                                                        >  SRR4302543.5659677/1‑94 (MQ=60)
    TAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTG                                                                                        >  SRR4302543.7614886/1‑94 (MQ=60)
      ATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGT                                                                                      >  SRR4302543.283700/1‑94 (MQ=60)
       TTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTC                                                                                     >  SRR4302543.6462832/1‑94 (MQ=60)
       TTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGGC                                                                                     >  SRR4302543.4556589/1‑94 (MQ=60)
        TAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCA                                                                                    >  SRR4302543.6592131/1‑94 (MQ=60)
          ATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATA                                                                                  >  SRR4302543.7551639/1‑94 (MQ=60)
          ATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATA                                                                                  >  SRR4302543.1506614/1‑94 (MQ=60)
            ATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATT                                                                                >  SRR4302543.7559616/1‑94 (MQ=60)
            ATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATT                                                                                >  SRR4302543.1654978/1‑94 (MQ=60)
            ATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGGACTGGTCATATT                                                                                >  SRR4302543.2958102/1‑94 (MQ=60)
             TATAATACATAGGGAATGTAAATGAACTAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTG                                                                               >  SRR4302543.400013/1‑94 (MQ=60)
             TATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTG                                                                               >  SRR4302543.5016216/1‑94 (MQ=60)
             TATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTG                                                                               >  SRR4302543.400014/1‑94 (MQ=60)
             TATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTG                                                                               >  SRR4302543.6689114/1‑94 (MQ=60)
              ATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGA                                                                              >  SRR4302543.2486222/1‑94 (MQ=60)
               TAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAA                                                                             >  SRR4302543.413365/1‑94 (MQ=60)
               TAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCGACGTTAACTGCGTGTACTGGTCATATTGAA                                                                             >  SRR4302543.3281196/1‑94 (MQ=60)
                  TACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAAC                                                                          <  SRR4302543.5175602/94‑1 (MQ=60)
                   ACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCAATGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACC                                                                         >  SRR4302543.1633231/1‑94 (MQ=60)
                    CATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCG                                                                        >  SRR4302543.2417708/1‑94 (MQ=60)
                    CATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCG                                                                        >  SRR4302543.1340333/1‑94 (MQ=60)
                     ATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGT                                                                       >  SRR4302543.2415251/1‑94 (MQ=60)
                      TAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTG                                                                      >  SRR4302543.2418141/1‑94 (MQ=60)
                       AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGA                                                                     >  SRR4302543.7293679/1‑94 (MQ=60)
                       AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGA                                                                     >  SRR4302543.825213/1‑94 (MQ=60)
                       AGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGA                                                                     >  SRR4302543.3761357/1‑94 (MQ=60)
                        GGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGAT                                                                    >  SRR4302543.431771/1‑94 (MQ=60)
                         GGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATA                                                                   >  SRR4302543.5498239/1‑94 (MQ=60)
                         GGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATA                                                                   <  SRR4302543.1539212/94‑1 (MQ=60)
                          GAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAA                                                                  >  SRR4302543.5540542/1‑94 (MQ=60)
                           AATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAG                                                                 <  SRR4302543.6891500/94‑1 (MQ=60)
                           AATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAG                                                                 <  SRR4302543.5079425/94‑1 (MQ=60)
                            ATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGA                                                                >  SRR4302543.3425626/1‑94 (MQ=60)
                             TGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAA                                                               >  SRR4302543.3119428/1‑94 (MQ=60)
                             TGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGGGCCGTACTGGGTACGTTAACTGCGTGGACGGGGCATATTGAAAACCGTGATAAGAA                                                               >  SRR4302543.3119427/1‑94 (MQ=60)
                              GTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAAC                                                              >  SRR4302543.2612597/1‑94 (MQ=60)
                              GTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAAC                                                              >  SRR4302543.7558668/1‑94 (MQ=60)
                              GTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAAC                                                              <  SRR4302543.3906807/94‑1 (MQ=60)
                                AAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTG                                                            >  SRR4302543.3020456/1‑94 (MQ=60)
                                 AATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGC                                                           >  SRR4302543.6295495/1‑94 (MQ=60)
                                  ATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCT                                                          >  SRR4302543.3219372/1‑94 (MQ=60)
                                    GAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCT                                                        <  SRR4302543.5306733/94‑1 (MQ=60)
                                    GAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCT                                                        >  SRR4302543.7257466/1‑94 (MQ=60)
                                     AACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTT                                                       >  SRR4302543.6753067/1‑94 (MQ=60)
                                     AACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTT                                                       >  SRR4302543.234455/1‑94 (MQ=60)
                                     AACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTT                                                       >  SRR4302543.6220238/1‑94 (MQ=60)
                                      ACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTA                                                      >  SRR4302543.3068961/1‑94 (MQ=60)
                                       CAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTAC                                                     <  SRR4302543.3423084/94‑1 (MQ=60)
                                        AAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACG                                                    >  SRR4302543.4379339/1‑94 (MQ=60)
                                        AAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACG                                                    >  SRR4302543.4379122/1‑94 (MQ=60)
                                          ATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTAAGAC                                                  >  SRR4302543.368241/1‑94 (MQ=60)
                                           TTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACT                                                 <  SRR4302543.4339231/94‑1 (MQ=60)
                                           TTTATTAAAGTTGCACTGGTAGGGGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACT                                                 >  SRR4302543.3119648/1‑94 (MQ=60)
                                            TTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTA                                                >  SRR4302543.389537/1‑94 (MQ=60)
                                             TATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTAC                                               >  SRR4302543.360580/1‑94 (MQ=60)
                                              ATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACC                                              >  SRR4302543.2548393/1‑94 (MQ=60)
                                              ATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACC                                              >  SRR4302543.6762588/1‑94 (MQ=60)
                                                 AAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGC                                           >  SRR4302543.3044909/1‑94 (MQ=60)
                                                 AAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGC                                           >  SRR4302543.5198258/1‑94 (MQ=60)
                                                 AAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGC                                           >  SRR4302543.6667703/1‑94 (MQ=60)
                                                     TTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCA                                       >  SRR4302543.6287653/1‑94 (MQ=60)
                                                     TTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCA                                       >  SRR4302543.6854991/1‑94 (MQ=60)
                                                      TGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAC                                      >  SRR4302543.7706955/1‑94 (MQ=60)
                                                      TGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCAC                                      >  SRR4302543.7422079/1‑94 (MQ=60)
                                                       GCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACC                                     >  SRR4302543.3971599/1‑94 (MQ=60)
                                                       GCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACC                                     <  SRR4302543.7418767/94‑1 (MQ=60)
                                                        CACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCC                                    >  SRR4302543.6021331/1‑94 (MQ=60)
                                                        CACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCC                                    >  SRR4302543.1082039/1‑94 (MQ=60)
                                                        CACTGGTAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCC                                    >  SRR4302543.1923749/1‑94 (MQ=60)
                                                              TAGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAAT                              <  SRR4302543.4920021/94‑1 (MQ=60)
                                                               AGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATT                             >  SRR4302543.5743847/1‑94 (MQ=60)
                                                               AGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATT                             >  SRR4302543.1167541/1‑94 (MQ=60)
                                                               AGGTGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATT                             >  SRR4302543.3854492/1‑94 (MQ=60)
                                                                  TGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCT                          >  SRR4302543.4005320/1‑94 (MQ=60)
                                                                  TGCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCT                          >  SRR4302543.3685071/1‑94 (MQ=60)
                                                                   GCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTA                         >  SRR4302543.1483059/1‑94 (MQ=60)
                                                                   GCAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTA                         >  SRR4302543.220882/1‑94 (MQ=60)
                                                                    CAGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTAT                        >  SRR4302543.2222386/1‑94 (MQ=60)
                                                                     AGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATT                       >  SRR4302543.1035086/1‑94 (MQ=60)
                                                                     AGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATT                       >  SRR4302543.7661427/1‑94 (MQ=60)
                                                                     AGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATT                       >  SRR4302543.1763081/1‑94 (MQ=60)
                                                                     AGTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATT                       >  SRR4302543.3708799/1‑94 (MQ=60)
                                                                      GTACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTT                      >  SRR4302543.4144095/1‑94 (MQ=60)
                                                                       TACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTC                     >  SRR4302543.7483174/1‑94 (MQ=60)
                                                                        ACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCT                    >  SRR4302543.2873063/1‑94 (MQ=60)
                                                                        ACTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCT                    >  SRR4302543.7697466/1‑94 (MQ=60)
                                                                         CTGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTA                   >  SRR4302543.3593155/1‑94 (MQ=60)
                                                                          TGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAA                  >  SRR4302543.7567978/1‑94 (MQ=60)
                                                                          TGGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAA                  >  SRR4302543.4511206/1‑94 (MQ=60)
                                                                           GGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAA                 >  SRR4302543.2369005/1‑94 (MQ=60)
                                                                           GGCTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCGCTTACGACTACCCGCTGCACCCGGCAACTTCTATTTCTAAA                 >  SRR4302543.7085432/1‑94 (MQ=60)
                                                                             CTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAAT               >  SRR4302543.7478024/1‑94 (MQ=60)
                                                                             CTACGTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAAT               >  SRR4302543.1733945/1‑94 (MQ=60)
                                                                                 GTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATT           >  SRR4302543.3500053/1‑94 (MQ=60)
                                                                                 GTTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATT           >  SRR4302543.3378624/1‑94 (MQ=60)
                                                                                  TTAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTG          >  SRR4302543.422209/1‑94 (MQ=60)
                                                                                   TAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGTCTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGG         <  SRR4302543.2236125/94‑1 (MQ=60)
                                                                                   TAACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGG         >  SRR4302543.2781624/1‑94 (MQ=60)
                                                                                    AACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGC        >  SRR4302543.4227907/1‑94 (MQ=60)
                                                                                    AACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGC        >  SRR4302543.897284/1‑94 (MQ=60)
                                                                                    AACTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGC        >  SRR4302543.5062937/1‑94 (MQ=60)
                                                                                      CTGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGG      >  SRR4302543.5202059/1‑94 (MQ=60)
                                                                                       TGCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGT     >  SRR4302543.7657942/1‑94 (MQ=60)
                                                                                        GCGTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTT    <  SRR4302543.819648/94‑1 (MQ=60)
                                                                                          GTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGC  >  SRR4302543.5924363/1‑94 (MQ=60)
                                                                                          GTGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGC  >  SRR4302543.5924362/1‑94 (MQ=60)
                                                                                          |                                                                                             
ATATTAATTAATATATAATACATAGGGAATGTAAATGAACAAATTTATTAAAGTTGCACTGGTAGGTGCAGTACTGGCTACGTTAACTGCATGTACTGGTCATATTGAAAACCGTGATAAGAACTGCTCTTACGACTACCTGCTGCACCCGGCAATTTCTATTTCTAAAATCATTGGCGGTTGC  >  NC_000913/3497051‑3497234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑