Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,749,710	T→C	L30P (CTT→CCT)	yiaU →	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,749,710	0	T	C	99.6%	784.1 / ‑5.1	246	L30P (CTT→CCT)	yiaU	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (229/16); total (230/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 250 total aligned reads displayed.

CGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGC  >  NC_000913/3749620‑3749799

 gATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTgg                                                                                        >  1:7100781/1‑93 (MQ=255)

  aTGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGc                                                                                       >  2:1034640/1‑93 (MQ=255)

   tGACAAAATTACAACTTAAGTACCGGGGGATAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCGACGCCGCGACTGTAGCTGGCa                                                                                      >  2:898217/1‑93 (MQ=255)

   tGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCa                                                                                      >  2:7062386/1‑93 (MQ=255)

     aCAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATc                                                                                    >  2:4173707/1‑93 (MQ=255)

      cAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCg                                                                                   >  1:592679/1‑93 (MQ=255)

         aaTTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGcac                                                                                >  1:1160399/1‑93 (MQ=255)

         aaTTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGcac                                                                                >  1:6272345/1‑93 (MQ=255)

         aaTTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGcac                                                                                >  1:4124845/1‑93 (MQ=255)

          aTTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGcaca                                                                               >  1:5413294/1‑93 (MQ=255)

              cAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGcc                                                                           >  1:1885497/1‑93 (MQ=255)

                aCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCaa                                                                         >  1:688319/1‑93 (MQ=255)

                aCTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCaa                                                                         >  1:4701373/1‑93 (MQ=255)

                 cTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAAc                                                                        >  1:5576526/1‑93 (MQ=255)

                  ttAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACg                                                                       >  1:4850862/1‑93 (MQ=255)

                  ttAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACg                                                                       >  2:4399401/1‑93 (MQ=255)

                      gTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAACCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAg                                                                   >  1:5078380/1‑93 (MQ=255)

                       tACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGc                                                                  >  1:2904380/1‑93 (MQ=255)

                         ccGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCaa                                                                >  2:7657145/1‑93 (MQ=255)

                         ccGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCaa                                                                >  1:341265/1‑93 (MQ=255)

                          cGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCaaa                                                               >  2:3852455/1‑93 (MQ=255)

                          cGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCaaa                                                               <  1:4238516/93‑1 (MQ=255)

                            ggAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTTAGCAAata                                                             >  2:2309204/1‑93 (MQ=255)

                             gAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCatatat                                                            >  1:7361764/1‑93 (MQ=255)

                             gAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAatat                                                            >  2:5768200/1‑93 (MQ=255)

                             gAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAatat                                                            >  1:2377206/1‑93 (MQ=255)

                             gAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAatat                                                            >  1:5639603/1‑93 (MQ=255)

                               gTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCt                                                          >  2:6063163/1‑93 (MQ=255)

                                ttAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCtt                                                         >  2:4000556/1‑93 (MQ=255)

                                ttAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCtt                                                         >  2:3315706/1‑93 (MQ=255)

                                    aaaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTg                                                     >  2:2910651/1‑93 (MQ=255)

                                    aaaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTg                                                     >  2:3835573/1‑93 (MQ=255)

                                     aaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGa                                                    >  1:3929738/1‑93 (MQ=255)

                                     aaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGa                                                    >  2:4179063/1‑93 (MQ=255)

                                     aaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGa                                                    >  2:2227686/1‑93 (MQ=255)

                                     aaTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGa                                                    >  2:55324/1‑93 (MQ=255)

                                      aTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAt                                                   >  1:5172217/1‑93 (MQ=255)

                                      aTTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAt                                                   >  2:4311964/1‑93 (MQ=255)

                                       ttATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAtt                                                  >  1:1505275/1‑93 (MQ=255)

                                        tATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAttt                                                 >  2:1758341/1‑93 (MQ=255)

                                         aTCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAtttt                                                >  2:6663782/1‑93 (MQ=255)

                                         aTCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAtttt                                                >  2:2015522/1‑93 (MQ=255)

                                          tctcGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTg                                               >  1:2279546/1‑93 (MQ=255)

                                          tctcGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTg                                               >  2:7120979/1‑93 (MQ=255)

                                          tctcGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTg                                               >  2:5969440/1‑93 (MQ=255)

                                          tctcGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTg                                               >  2:1591352/1‑93 (MQ=255)

                                            tcGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGaa                                             >  1:2803454/1‑93 (MQ=255)

                                              ggTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATc                                           >  1:315061/1‑93 (MQ=255)

                                               gTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCa                                          >  2:3566828/1‑93 (MQ=255)

                                               gTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCa                                          >  2:2522871/1‑93 (MQ=255)

                                                 aaTCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCaaa                                        >  2:6053203/1‑93 (MQ=255)

                                                  aTCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCaaaa                                       >  2:124869/1‑93 (MQ=255)

                                                  aTCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCaaaa                                       >  2:7244/1‑93 (MQ=255)

                                                    cGCTGCCCGTGAAAATATCAGCCATTCCGCGACTGTACCTGGCAACGCACAGGCCAACGTCAGCAAATATCTTGCTGATTGTGAATCaaaata                                     >  2:4471894/1‑91 (MQ=255)

                                                     gCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTg                                    >  1:4557459/1‑93 (MQ=255)

                                                     gCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTg                                    >  2:2653278/1‑93 (MQ=255)

                                                      cTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTgg                                   >  2:6133318/1‑93 (MQ=255)

                                                       tGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTggg                                  <  2:1893541/93‑1 (MQ=255)

                                                         ccAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGtt                                >  2:2412739/1‑93 (MQ=255)

                                                          cAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGttt                               >  2:652250/1‑93 (MQ=255)

                                                          cAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGttt                               >  1:676446/1‑93 (MQ=255)

                                                          cAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGttt                               >  2:1689000/1‑93 (MQ=255)

                                                           aGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTa                              >  1:4322643/1‑93 (MQ=255)

                                                            gTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaa                             >  2:2820095/1‑93 (MQ=255)

                                                            gTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaa                             >  1:908142/1‑93 (MQ=255)

                                                             tGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGGTTaaa                            >  1:3577120/1‑93 (MQ=255)

                                                              gAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaaa                           <  1:1794188/93‑1 (MQ=255)

                                                              gAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTaaaa                           >  2:7365477/1‑93 (MQ=255)

                                                              gAAAATATCAGCCATGCAGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGATTaaaa                           >  2:2141530/1‑93 (MQ=255)

                                                                aaaTATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGt                         >  2:3133210/1‑93 (MQ=255)

                                                                 aaTATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTc                        >  1:7067933/1‑93 (MQ=255)

                                                                 aaTATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTc                        >  1:2229949/1‑93 (MQ=255)

                                                                  atatCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCt                       >  2:7585989/1‑93 (MQ=255)

                                                                  atatCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCt                       >  1:2163062/1‑93 (MQ=255)

                                                                    atCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCttt                     <  2:7676859/93‑1 (MQ=255)

                                                                     tCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTg                    >  2:757590/1‑93 (MQ=255)

                                                                     tCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTg                    >  2:2351203/1‑93 (MQ=255)

                                                                      cAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGa                   >  1:7848815/1‑93 (MQ=255)

                                                                       aGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAc                  >  1:1082272/1‑93 (MQ=255)

                                                                       aGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAc                  >  2:5243469/1‑93 (MQ=255)

                                                                        gCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAcc                 >  1:1936680/1‑93 (MQ=255)

                                                                          cATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGa               >  2:7872040/1‑93 (MQ=255)

                                                                          cATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGa               >  1:7578442/1‑93 (MQ=255)

                                                                          cATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGa               >  2:376747/1‑93 (MQ=255)

                                                                          cATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGa               >  2:3384853/1‑93 (MQ=255)

                                                                          cATGCCGCGACTGTACCTGGCAGCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGa               >  1:1335805/1‑93 (MQ=255)

                                                                           aTGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGt                         <  1:7382296/82‑1 (MQ=255)

                                                                           aTGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGt                         >  2:7382295/1‑82 (MQ=255)

                                                                            tGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAac             >  1:7271933/1‑93 (MQ=255)

                                                                            tGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACAGAac             >  1:6456911/1‑93 (MQ=255)

                                                                               cgcgACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAAAATCTTGCTGATTTTGTATCAAAAGTGGGGTTAAAACACTTTAACGGAaccac          >  2:5144389/1‑93 (MQ=255)

                                                                               cgcgACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccac          >  2:4941547/1‑93 (MQ=255)

                                                                                gcgACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccacc         >  1:1643410/1‑93 (MQ=255)

                                                                                gcgACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAaccacc         >  1:6142586/1‑93 (MQ=255)

                                                                                   aCTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCgg      >  1:605913/1‑93 (MQ=255)

                                                                                   aCTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCgg      >  2:6417570/1‑93 (MQ=255)

                                                                                   aCTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCgg      >  1:4385335/1‑93 (MQ=255)

                                                                                     tGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCa    >  2:5061005/1‑93 (MQ=255)

                                                                                      gTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAg   >  2:4725543/1‑93 (MQ=255)

                                                                                       tACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGc  >  2:3231793/1‑93 (MQ=255)

CGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGC  >  NC_000913/3749620‑3749799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGAT  >  NC_000913/3749617‑3749803

TTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACC                                                                                               >  SRR4302543.1910570/1‑94 (MQ=60)

 TGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCT                                                                                              >  SRR4302543.1967657/1‑94 (MQ=60)

  GCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTG                                                                                             >  SRR4302543.4127678/1‑94 (MQ=60)

   CGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGG                                                                                            >  SRR4302543.7169741/1‑94 (MQ=60)

    GATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTATTCGCTGCCAGTGAAAATATCAGCCATGCCGCGACAGTACCTGGC                                                                                           >  SRR4302543.7068995/1‑94 (MQ=60)

    GATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGC                                                                                           >  SRR4302543.5485281/1‑94 (MQ=60)

     ATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCA                                                                                          >  SRR4302543.4910851/1‑94 (MQ=60)

      TGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCAT                                                                                         >  SRR4302543.4326117/1‑94 (MQ=60)

        ACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCG                                                                                       >  SRR4302543.598120/1‑94 (MQ=60)

        ACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCG                                                                                       >  SRR4302543.5224437/1‑94 (MQ=60)

        ACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCG                                                                                       >  SRR4302543.754555/1‑94 (MQ=60)

         CAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGC                                                                                      >  SRR4302543.3536264/1‑94 (MQ=60)

           AAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCAC                                                                                    >  SRR4302543.4162030/1‑94 (MQ=60)

           AAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCAC                                                                                    >  SRR4302543.4793139/1‑94 (MQ=60)

           AAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCAC                                                                                    >  SRR4302543.6331486/1‑94 (MQ=60)

           AAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCAC                                                                                    >  SRR4302543.1170972/1‑94 (MQ=60)

            AATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACA                                                                                   >  SRR4302543.4840615/1‑94 (MQ=60)

            AATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACA                                                                                   >  SRR4302543.5462535/1‑94 (MQ=60)

             ATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAG                                                                                  <  SRR4302543.2244030/94‑1 (MQ=60)

             ATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAG                                                                                  >  SRR4302543.2350137/1‑94 (MQ=60)

                ACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCC                                                                               >  SRR4302543.1902442/1‑94 (MQ=60)

                 CAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCA                                                                              >  SRR4302543.2741417/1‑94 (MQ=60)

                 CAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCA                                                                              >  SRR4302543.2741416/1‑94 (MQ=60)

                  AACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAA                                                                             >  SRR4302543.4744315/1‑94 (MQ=60)

                  AACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAA                                                                             >  SRR4302543.694648/1‑94 (MQ=60)

                   ACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAAC                                                                            >  SRR4302543.5627289/1‑94 (MQ=60)

                    CTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACG                                                                           >  SRR4302543.2097452/1‑94 (MQ=60)

                    CTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACG                                                                           >  SRR4302543.4895096/1‑94 (MQ=60)

                        AGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAACCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAG                                                                       >  SRR4302543.5124454/1‑94 (MQ=60)

                         GTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGC                                                                      >  SRR4302543.2930784/1‑94 (MQ=60)

                           ACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAA                                                                    >  SRR4302543.344404/1‑94 (MQ=60)

                            CCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAA                                                                   >  SRR4302543.2879424/1‑94 (MQ=60)

                             CGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCACCGTCAGCAAAT                                                                  >  SRR4302543.2273702/1‑94 (MQ=60)

                             CGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAAT                                                                  >  SRR4302543.2273703/1‑94 (MQ=60)

                             CGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAAT                                                                  <  SRR4302543.4276964/94‑1 (MQ=60)

                              GGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCNTCNNACAGGCCAACGNCANCNANTA                                                                 >  SRR4302543.247695/1‑94 (MQ=60)

                               GGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCATATAT                                                                >  SRR4302543.7432557/1‑94 (MQ=60)

                               GGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATAT                                                                >  SRR4302543.2398739/1‑94 (MQ=60)

                               GGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATAT                                                                >  SRR4302543.5691018/1‑94 (MQ=60)

                               GGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATAT                                                                >  SRR4302543.7432562/1‑94 (MQ=60)

                               GGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATAT                                                                >  SRR4302543.1625097/1‑94 (MQ=60)

                                  GTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTT                                                             >  SRR4302543.6478019/1‑94 (MQ=60)

                                    TAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGC                                                           >  SRR4302543.3526961/1‑94 (MQ=60)

                                       AAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGA                                                        >  SRR4302543.3964431/1‑94 (MQ=60)

                                        AATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAT                                                       >  SRR4302543.5218927/1‑94 (MQ=60)

                                        AATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGAT                                                       >  SRR4302543.5476682/1‑94 (MQ=60)

                                         ATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATT                                                      >  SRR4302543.1518799/1‑94 (MQ=60)

                                         ATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATT                                                      >  SRR4302543.6893211/1‑94 (MQ=60)

                                         ATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGACAGCAAATATCTTGCTGATT                                                      >  SRR4302543.6153691/1‑94 (MQ=60)

                                           TATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTT                                                    >  SRR4302543.1275859/1‑94 (MQ=60)

                                            ATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTG                                                   >  SRR4302543.2300389/1‑94 (MQ=60)

                                              CTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAA                                                 >  SRR4302543.2829091/1‑94 (MQ=60)

                                               TCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAAT                                                <  SRR4302543.6076016/94‑1 (MQ=60)

                                                CGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACCGGCCAACGTCAGCCAATATCTTGCTGATTTTGAATA                                               >  SRR4302543.317908/1‑94 (MQ=60)

                                                CGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCCACGTCAGCAAATATCTTGCTGATTTTGAATC                                               >  SRR4302543.4168125/1‑94 (MQ=60)

                                                CGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATC                                               >  SRR4302543.317907/1‑94 (MQ=60)

                                                CGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATC                                               >  SRR4302543.7768516/1‑94 (MQ=60)

                                                   TAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAA                                            >  SRR4302543.892555/1‑94 (MQ=60)

                                                    AATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAA                                           >  SRR4302543.3920126/1‑94 (MQ=60)

                                                      TCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGG                                         >  SRR4302543.1224918/1‑94 (MQ=60)

                                                       CGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTG                                        >  SRR4302543.4598953/1‑94 (MQ=60)

                                                       CGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTG                                        >  SRR4302543.1548194/1‑94 (MQ=60)

                                                        GCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGG                                       >  SRR4302543.1768335/1‑94 (MQ=60)

                                                         CTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGG                                      >  SRR4302543.5149062/1‑94 (MQ=60)

                                                          TGCCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGT                                     >  SRR4302543.3149935/1‑94 (MQ=60)

                                                            CCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTT                                   >  SRR4302543.5869230/1‑94 (MQ=60)

                                                            CCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTT                                   >  SRR4302543.1446047/1‑94 (MQ=60)

                                                            CCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTT                                   >  SRR4302543.7349178/1‑94 (MQ=60)

                                                            CCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTT                                   >  SRR4302543.5869434/1‑94 (MQ=60)

                                                            CCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTT                                   >  SRR4302543.682670/1‑94 (MQ=60)

                                                            CCAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTT                                   >  SRR4302543.6387633/1‑94 (MQ=60)

                                                             CAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTA                                  >  SRR4302543.5148214/1‑94 (MQ=60)

                                                             CAGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTA                                  >  SRR4302543.4361895/1‑94 (MQ=60)

                                                              AGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAA                                 >  SRR4302543.4553384/1‑94 (MQ=60)

                                                              AGTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAA                                 >  SRR4302543.916135/1‑94 (MQ=60)

                                                               GTGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGGTTAAA                                >  SRR4302543.3608728/1‑94 (MQ=60)

                                                                TGAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAA                               >  SRR4302543.7136942/1‑94 (MQ=60)

                                                                 GAAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAG                              <  SRR4302543.1810240/94‑1 (MQ=60)

                                                                  AAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGT                             >  SRR4302543.1094333/1‑94 (MQ=60)

                                                                  AAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGT                             >  SRR4302543.1925453/1‑94 (MQ=60)

                                                                  AAAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGT                             >  SRR4302543.6518538/1‑94 (MQ=60)

                                                                   tctccgctctCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTC                            <  SRR4302543.7453248/84‑1 (MQ=60)

                                                                   AAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTC                            >  SRR4302543.181689/1‑94 (MQ=60)

                                                                   AAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTC                            >  SRR4302543.7136558/1‑94 (MQ=60)

                                                                   AAATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTC                            >  SRR4302543.2250093/1‑94 (MQ=60)

                                                                    AATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCT                           >  SRR4302543.2182449/1‑94 (MQ=60)

                                                                    AATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCT                           >  SRR4302543.2033407/1‑94 (MQ=60)

                                                                    AATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCT                           >  SRR4302543.4107229/1‑94 (MQ=60)

                                                                    AATATCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCATGCTGATTTTGAATCATAAGTGGGTTTAAAAGTCT                           >  SRR4302543.6271325/1‑94 (MQ=60)

                                                                        TCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGA                       >  SRR4302543.1897705/1‑94 (MQ=60)

                                                                        TCAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGA                       >  SRR4302543.7922779/1‑94 (MQ=60)

                                                                         CAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAC                      >  SRR4302543.1092000/1‑94 (MQ=60)

                                                                         CAGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGAC                      >  SRR4302543.4388830/1‑94 (MQ=60)

                                                                          AGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACC                     >  SRR4302543.871284/1‑94 (MQ=60)

                                                                          AGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACC                     >  SRR4302543.6856140/1‑94 (MQ=60)

                                                                          AGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACC                     >  SRR4302543.1954088/1‑94 (MQ=60)

                                                                          AGCCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACC                     >  SRR4302543.4290606/1‑94 (MQ=60)

                                                                            CCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGA                   >  SRR4302543.7650502/1‑94 (MQ=60)

                                                                            CCATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGA                   >  SRR4302543.5636540/1‑94 (MQ=60)

                                                                            CCATGCCGCGACTGTACCTGGCAGCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGA                   >  SRR4302543.1347718/1‑94 (MQ=60)

                                                                             CATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAA                  >  SRR4302543.714200/1‑94 (MQ=60)

                                                                              ATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAAC                 >  SRR4302543.7342177/1‑94 (MQ=60)

                                                                              ATGCCGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACAGAAC                 >  SRR4302543.6517748/1‑94 (MQ=60)

                                                                                  CGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACC             >  SRR4302543.6187440/1‑94 (MQ=60)

                                                                                  CGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACC             >  SRR4302543.6200764/1‑94 (MQ=60)

                                                                                  CGCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACC             >  SRR4302543.1658083/1‑94 (MQ=60)

                                                                                   GCGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCC            <  SRR4302543.7229186/94‑1 (MQ=60)

                                                                                    CGACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCG           <  SRR4302543.7546613/94‑1 (MQ=60)

                                                                                     GACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGG          >  SRR4302543.611453/1‑94 (MQ=60)

                                                                                     GACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGG          >  SRR4302543.4425095/1‑94 (MQ=60)

                                                                                      ACTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGC         <  SRR4302543.4663614/94‑1 (MQ=60)

                                                                                       CTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCA        >  SRR4302543.4489868/1‑94 (MQ=60)

                                                                                       CTGTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCA        >  SRR4302543.2991054/1‑94 (MQ=60)

                                                                                         GTACCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGC      >  SRR4302543.7377372/1‑94 (MQ=60)

                                                                                            CCTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGA   >  SRR4302543.2935140/1‑94 (MQ=60)

                                                                                             CTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGAT  >  SRR4302543.6612856/1‑94 (MQ=60)

TTGCGATGACAAAATTACAACTTAAGTACCGGGAGTTAAAAATTATCTCGGTAATCGCTGCCAGTGAAAATATCAGCCATGCCGCGACTGTACTTGGCATCGCACAGGCCAACGTCAGCAAATATCTTGCTGATTTTGAATCAAAAGTGGGTTTAAAAGTCTTTGACCGAACCACCCGGCAGCTGAT  >  NC_000913/3749617‑3749803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`