Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,870,765 +G coding (476/1764 nt) dgoA ← 2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,870,7641.G99.3% 1354.4 / 0.1 431coding (477/1764 nt)dgoA2‑oxo‑3‑deoxygalactonate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (209/218);  total (211/220)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 477 total aligned reads displayed.

GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCT‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG  >  NC_000913/3870672‑3870851
                                                                                               |                                                                                       
gcgGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCt                                                                                          <  2:5169157/93‑1 (MQ=255)
  ggTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCCTGCACAACCTGCGTCTCTCCACTGCGCCA‑GGTTTTCT‑g                                                                                        >  2:405476/1‑93 (MQ=255)
   gTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCT‑ggc                                                                                      <  1:6044183/93‑3 (MQ=255)
    tGCCCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCT‑ggcg                                                                                     <  1:4367093/93‑4 (MQ=255)
       gcTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCa                                                                                   >  1:5404004/1‑93 (MQ=255)
       gcTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCa                                                                                   <  2:1838649/93‑1 (MQ=255)
        ctctACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCAc                                                                                  <  1:1894681/93‑1 (MQ=255)
          ctACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCAcgc                                                                                >  2:2163707/1‑93 (MQ=255)
           tACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTGTTCTGGCGGCAcgcc                                                                               >  2:7026542/1‑93 (MQ=255)
             cGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCAcgccgc                                                                             <  1:7620488/93‑1 (MQ=255)
               gATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCa                                                                           >  2:1983580/1‑93 (MQ=255)
               gATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCa                                                                           <  2:5043410/93‑1 (MQ=255)
                 ttGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAAc                                                                         >  2:2949035/1‑93 (MQ=255)
                 ttGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAAc                                                                         >  2:6905982/1‑93 (MQ=255)
                     ccGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCa                                                                     >  2:4457825/1‑93 (MQ=255)
                     ccGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCa                                                                     <  2:1241437/93‑1 (MQ=255)
                      cGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCaa                                                                    >  1:936369/1‑93 (MQ=255)
                       ggCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCaaa                                                                   >  2:3849012/1‑93 (MQ=255)
                          gcgATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAc                                                                >  2:4657976/1‑93 (MQ=255)
                          gcgATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAc                                                                <  2:2733091/93‑1 (MQ=255)
                          gcgATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGAc                                                                <  1:6220129/93‑1 (MQ=255)
                             aTAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGc                                                             >  2:3017337/1‑93 (MQ=255)
                              tAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCg                                                            >  2:1316630/1‑93 (MQ=255)
                              tAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCg                                                            >  1:6929630/1‑93 (MQ=255)
                               agagATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGa                                                           <  1:4490400/93‑1 (MQ=255)
                               agagATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGa                                                           <  1:6711781/93‑1 (MQ=255)
                                gagaTCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAt                                                          <  1:2754441/93‑1 (MQ=255)
                                gagaTCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAt                                                          <  1:2494304/93‑1 (MQ=255)
                                aagaTCGCTACCTAAGCCCG‑CCCATGCACAACCTGCGTCTATCCACTGCCACA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGAt                                                          <  2:7933727/92‑1 (MQ=255)
                                  gaTCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGt                                                        >  1:2119585/1‑93 (MQ=255)
                                   aTCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTc                                                       >  2:2367493/1‑93 (MQ=255)
                                   aTCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTc                                                       <  1:7883766/93‑1 (MQ=255)
                                   aTCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTc                                                       <  1:7077741/93‑1 (MQ=255)
                                    tCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                                      <  2:4207164/93‑1 (MQ=255)
                                    tCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTcc                                                      >  1:5891608/1‑93 (MQ=255)
                                      gCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGa                                                    <  1:718104/93‑1 (MQ=255)
                                         gCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATgg                                                 <  2:2282497/93‑1 (MQ=255)
                                           cTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCa                                               >  2:5781315/1‑93 (MQ=255)
                                           cTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCa                                               >  1:3856422/1‑93 (MQ=255)
                                            tAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCTAAGACTGCGATGTCCGATGGCat                                              >  2:5415857/1‑92 (MQ=255)
                                            tAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCaa                                              >  2:4852033/1‑93 (MQ=255)
                                             aaGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAAt                                             <  1:7267898/93‑1 (MQ=255)
                                              aGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATa                                            <  2:6242743/93‑1 (MQ=255)
                                               gcccg‑cccCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATAc                                           <  1:1313871/93‑1 (MQ=255)
                                                cccg‑cccCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATAcc                                          <  1:1383306/93‑1 (MQ=255)
                                                  cg‑cccCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGc                                        <  2:6548145/93‑1 (MQ=255)
                                                   g‑cccCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCt                                       >  2:7470232/1‑93 (MQ=255)
                                                      cccTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCttt                                     >  2:1940458/1‑93 (MQ=255)
                                                      cccTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCttt                                     <  1:1609069/93‑1 (MQ=255)
                                                        cTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTa                                   >  2:4366827/1‑93 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  >  1:4807564/1‑93 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  >  1:5309445/1‑93 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  >  1:3316990/1‑93 (MQ=255)
                                                         tGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTaa                                  >  1:1502191/1‑93 (MQ=255)
                                                         cgtgcaACCGGCGGTTATTCACGGTGCCATGGTTTGTG‑GCGGCACGCCGCCAACGGCAAAGACTGCGATGGCCGATGGCAATACCGCTTTTaa                                  <  1:2000532/89‑1 (MQ=255)
                                                          gCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAc                                 <  1:3417245/93‑1 (MQ=255)
                                                             caACCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCt                              >  1:5026513/1‑93 (MQ=255)
                                                              aaCCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCtt                             >  1:5175204/1‑93 (MQ=255)
                                                              aaCCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCtt                             <  2:1741035/93‑1 (MQ=255)
                                                              aaCCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCtt                             <  2:7347027/93‑1 (MQ=255)
                                                               aCCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt                            <  2:3362282/93‑1 (MQ=255)
                                                               aCCTGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCttt                            <  1:4644255/93‑1 (MQ=255)
                                                                  tGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAt                         >  1:6654653/1‑93 (MQ=255)
                                                                  tGCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAt                         >  1:5471312/1‑93 (MQ=255)
                                                                   gCGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATg                        <  2:1086184/93‑1 (MQ=255)
                                                                    cGTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGt                       <  1:4169359/93‑1 (MQ=255)
                                                                     gTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTa                      <  2:6457621/93‑1 (MQ=255)
                                                                     gTCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTa                      >  2:1513529/1‑93 (MQ=255)
                                                                      tCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAt                     <  2:6813602/93‑1 (MQ=255)
                                                                      tCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAt                     >  2:877360/1‑93 (MQ=255)
                                                                      tCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAt                     <  2:3539294/93‑1 (MQ=255)
                                                                      tCTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAt                     >  1:7492640/1‑93 (MQ=255)
                                                                       cTATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAtt                    <  2:5996787/93‑1 (MQ=255)
                                                                       cTAACCACGGAGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAtt                    <  2:1431327/93‑1 (MQ=255)
                                                                        tATCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTg                   <  1:6444102/93‑1 (MQ=255)
                                                                         aTCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGc                  <  1:6528931/93‑1 (MQ=255)
                                                                         aTCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGc                  >  2:5502308/1‑93 (MQ=255)
                                                                          tCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCg                 >  1:3549747/1‑93 (MQ=255)
                                                                          tCCACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCg                 >  1:5574545/1‑93 (MQ=255)
                                                                            cACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCggc               >  2:2476452/1‑92 (MQ=255)
                                                                            cACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGa               <  1:768890/93‑1 (MQ=255)
                                                                            cACTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGa               >  1:4242808/1‑93 (MQ=255)
                                                                             aCTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGAc              >  2:7206379/1‑93 (MQ=255)
                                                                              cTGCGCCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGAcc             >  2:3193708/1‑93 (MQ=255)
                                                                                  gcaa‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  2:3278430/90‑1 (MQ=255)
                                                                                  gcCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  1:1224124/93‑1 (MQ=255)
                                                                                  gcCA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCaaaa         <  2:3627658/93‑1 (MQ=255)
                                                                                    cA‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGc       >  1:4072325/1‑93 (MQ=255)
                                                                                     a‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGcc      >  2:7804647/1‑93 (MQ=255)
                                                                                     a‑GGTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGcc      <  1:873741/93‑1 (MQ=255)
                                                                                       ggTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCg     >  2:7599837/1‑93 (MQ=255)
                                                                                       ggTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCg     >  1:1004271/1‑93 (MQ=255)
                                                                                       ggTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCg     <  2:6149023/93‑1 (MQ=255)
                                                                                        gTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGa    >  1:1766137/1‑93 (MQ=255)
                                                                                        gTTTTCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGa    >  1:979390/1‑93 (MQ=255)
                                                                                         ttttCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAt   <  2:5558087/93‑1 (MQ=255)
                                                                                         ttttCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAt   <  2:5351814/93‑1 (MQ=255)
                                                                                         ttttCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAt   >  2:4964136/1‑93 (MQ=255)
                                                                                          tttCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATg  >  1:3634925/1‑93 (MQ=255)
                                                                                          tttCTGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATg  <  1:3190211/93‑1 (MQ=255)
                                                                                               |                                                                                       
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCG‑CCCCTGCACAACCTGCGTCTATCCACTGCGCCA‑GGTTTTCT‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG  >  NC_000913/3870672‑3870851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 244 total aligned reads displayed.
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAAA  >  NC_000913/3870672‑3870858
                                                                                               |                                                                                             
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑G                                                                                               >  SRR4302543.2504661/1‑94 (MQ=60)
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑G                                                                                               >  SRR4302543.921963/1‑94 (MQ=60)
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑G                                                                                               >  SRR4302543.7313779/1‑94 (MQ=60)
 CGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GG                                                                                              <  SRR4302543.1206228/94‑1 (MQ=60)
 CGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GG                                                                                              <  SRR4302543.2977400/94‑1 (MQ=60)
 CGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GG                                                                                              <  SRR4302543.1541988/94‑1 (MQ=60)
 CGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GG                                                                                              >  SRR4302543.7757947/1‑94 (MQ=60)
   GTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑Ggcg                                                                                            <  SRR4302543.6101394/94‑4 (MQ=60)
    TGCCCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGT                                                                                            <  SRR4302543.4406654/94‑1 (MQ=60)
     GCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTC                                                                                           >  SRR4302543.4675915/1‑94 (MQ=60)
     GCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTC                                                                                           >  SRR4302543.4707864/1‑94 (MQ=60)
      GGCTCTACGGATTGCCCGCCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCA                                                                                          <  SRR4302543.986858/94‑1 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCA                                                                                          >  SRR4302543.5453135/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCA                                                                                          >  SRR4302543.1836541/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCA                                                                                          >  SRR4302543.4131234/1‑94 (MQ=60)
      CGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCA                                                                                          <  SRR4302543.4012291/94‑1 (MQ=60)
       GCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAC                                                                                         >  SRR4302543.1315088/1‑94 (MQ=60)
       GCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCAC                                                                                         <  SRR4302543.5971685/94‑1 (MQ=60)
        CTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACG                                                                                        >  SRR4302543.1487331/1‑94 (MQ=60)
        CTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACG                                                                                        <  SRR4302543.3630069/94‑1 (MQ=60)
        CTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACG                                                                                        >  SRR4302543.4213421/1‑94 (MQ=60)
        CTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACG                                                                                        <  SRR4302543.1911718/94‑1 (MQ=60)
          CTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCC                                                                                      >  SRR4302543.423837/1‑94 (MQ=60)
             CGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCC                                                                                   <  SRR4302543.7692812/94‑1 (MQ=60)
             CGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCC                                                                                   <  SRR4302543.722762/94‑1 (MQ=60)
              GGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGTGCCAGGTTTTC‑TGGCGTCACGCCGCCA                                                                                  >  SRR4302543.5192032/1‑94 (MQ=60)
              GGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCA                                                                                  >  SRR4302543.4185043/1‑94 (MQ=60)
              GGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCA                                                                                  >  SRR4302543.3384045/1‑94 (MQ=60)
               GATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAC                                                                                 >  SRR4302543.5318361/1‑94 (MQ=60)
                ATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAAC                                                                                <  SRR4302543.7064804/94‑1 (MQ=60)
                ATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAAC                                                                                <  SRR4302543.978584/94‑1 (MQ=60)
                ATGGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAAC                                                                                <  SRR4302543.108676/94‑1 (MQ=60)
                 TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACG                                                                               <  SRR4302543.6629115/94‑1 (MQ=60)
                 TTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACG                                                                               <  SRR4302543.895564/94‑1 (MQ=60)
                     CCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAA                                                                           >  SRR4302543.944553/1‑94 (MQ=60)
                     CCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAA                                                                           >  SRR4302543.333061/1‑94 (MQ=60)
                      CGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAA                                                                          >  SRR4302543.5382600/1‑94 (MQ=60)
                       ggcggtatagagatcccggcatagcCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAG                                                                         <  SRR4302543.1548757/69‑1 (MQ=60)
                       GGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAG                                                                         >  SRR4302543.655065/1‑94 (MQ=60)
                        GCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTCGG‑GCGTCACGCCGCCAACGGCAAAGA                                                                        <  SRR4302543.3499976/94‑1 (MQ=60)
                         CGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGAC                                                                       <  SRR4302543.5409192/94‑1 (MQ=60)
                          GCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACT                                                                      <  SRR4302543.6278846/94‑1 (MQ=60)
                           CGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCCAAGACTG                                                                     >  SRR4302543.5465529/1‑94 (MQ=60)
                           CGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTG                                                                     >  SRR4302543.5465530/1‑94 (MQ=60)
                             GTAGAGATCGCTGCCTAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCG                                                                   <  SRR4302543.7093623/94‑1 (MQ=60)
                             ATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCG                                                                   >  SRR4302543.4698383/1‑94 (MQ=60)
                             ATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCG                                                                   <  SRR4302543.1400802/94‑1 (MQ=60)
                             ATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCG                                                                   >  SRR4302543.6994806/1‑94 (MQ=60)
                             ATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCG                                                                   >  SRR4302543.7972422/1‑94 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                 <  SRR4302543.560255/94‑1 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                 <  SRR4302543.6775187/94‑1 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                 <  SRR4302543.2700797/94‑1 (MQ=60)
                               AGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGAT                                                                 <  SRR4302543.4531197/94‑1 (MQ=60)
                                GAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATG                                                                <  SRR4302543.2032521/94‑1 (MQ=60)
                                GAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATG                                                                <  SRR4302543.2779498/94‑1 (MQ=60)
                                GAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATG                                                                <  SRR4302543.2516928/94‑1 (MQ=60)
                                 AGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGT                                                               <  SRR4302543.5521453/94‑1 (MQ=60)
                                 AGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGT                                                               <  SRR4302543.1064409/94‑1 (MQ=60)
                                 AGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGT                                                               >  SRR4302543.2138558/1‑94 (MQ=60)
                                  GATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTC                                                              <  SRR4302543.667978/94‑1 (MQ=60)
                                  GATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTC                                                              <  SRR4302543.7545401/94‑1 (MQ=60)
                                   ATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCC                                                             >  SRR4302543.5945321/1‑94 (MQ=60)
                                   ATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCC                                                             <  SRR4302543.7957942/94‑1 (MQ=60)
                                   ATCGCTGACTAAGCCGGCGCCTGCACAACACGCGTCTGTCCACTGCGCCGGGTTTTC‑TGGCGTCGCGCTGCCAACGGCAAAGACTGCGATGTCC                                                             <  SRR4302543.5424886/94‑1 (MQ=36)
                                      GCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGAT                                                          <  SRR4302543.7986602/94‑1 (MQ=60)
                                      GCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGAT                                                          <  SRR4302543.724595/94‑1 (MQ=60)
                                       CTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                         >  SRR4302543.1911312/1‑94 (MQ=60)
                                       CTGCCCAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATG                                                         <  SRR4302543.1335179/94‑1 (MQ=60)
                                        TGCTTAAGCCCGCCCTTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGG                                                        <  SRR4302543.3385605/94‑1 (MQ=60)
                                        TGCCTAAGCCCGCCCCTGCACACCCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGG                                                        <  SRR4302543.5896551/94‑1 (MQ=60)
                                        TGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGG                                                        >  SRR4302543.4874512/1‑94 (MQ=60)
                                          CCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCA                                                      >  SRR4302543.4585931/1‑94 (MQ=60)
                                           CTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAA                                                     >  SRR4302543.6572221/1‑94 (MQ=60)
                                            TAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAAT                                                    <  SRR4302543.5520760/94‑1 (MQ=60)
                                             AAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATA                                                   <  SRR4302543.4176741/94‑1 (MQ=60)
                                             AAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATA                                                   <  SRR4302543.1302192/94‑1 (MQ=60)
                                             AAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATA                                                   <  SRR4302543.7338112/94‑1 (MQ=60)
                                              AGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATAC                                                  <  SRR4302543.7235073/94‑1 (MQ=60)
                                               GCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACC                                                 <  SRR4302543.1325548/94‑1 (MQ=60)
                                               GCCCGCCCCTGCACAACCTGCGTCTAGCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACC                                                 <  SRR4302543.3910744/94‑1 (MQ=60)
                                                CCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCG                                                <  SRR4302543.1395754/94‑1 (MQ=60)
                                                CCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCCTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCG                                                <  SRR4302543.7931750/94‑1 (MQ=60)
                                                 CCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGC                                               >  SRR4302543.3227560/1‑94 (MQ=60)
                                                 CCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGC                                               <  SRR4302543.1830402/94‑1 (MQ=60)
                                                  CGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCT                                              >  SRR4302543.3050234/1‑94 (MQ=60)
                                                   GCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTT                                             >  SRR4302543.6836872/1‑94 (MQ=60)
                                                    CCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTT                                            <  SRR4302543.4397716/94‑1 (MQ=60)
                                                    CCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTT                                            <  SRR4302543.889061/94‑1 (MQ=60)
                                                     CCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTT                                           >  SRR4302543.1256004/1‑94 (MQ=60)
                                                     CCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTT                                           <  SRR4302543.6866089/94‑1 (MQ=60)
                                                     CCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTT                                           <  SRR4302543.1623423/94‑1 (MQ=60)
                                                     CCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTT                                           >  SRR4302543.2666226/1‑94 (MQ=60)
                                                      CNTGCACAACCTGCGTCTATCCACNGCGCNAGGNTNTC‑TNGCGTCANGCCGNCNACGNNAAAGACnncnannnnngntnnanatnnnnnntnnn                                          >  SRR4302543.1561589/1‑65 (MQ=39)
                                                      CCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTA                                          <  SRR4302543.7206189/94‑1 (MQ=60)
                                                      CCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTA                                          >  SRR4302543.7012594/1‑94 (MQ=60)
                                                       CTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAA                                         >  SRR4302543.3885815/1‑94 (MQ=60)
                                                       CTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAA                                         >  SRR4302543.3346923/1‑94 (MQ=60)
                                                       CTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAA                                         >  SRR4302543.4851472/1‑94 (MQ=60)
                                                       CTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAA                                         >  SRR4302543.1515692/1‑94 (MQ=60)
                                                        TGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAC                                        <  SRR4302543.2018493/94‑1 (MQ=60)
                                                        TGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAAC                                        <  SRR4302543.1392666/94‑1 (MQ=60)
                                                         GCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACG                                       <  SRR4302543.5976662/94‑1 (MQ=60)
                                                         GCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACG                                       <  SRR4302543.3447831/94‑1 (MQ=60)
                                                         GCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACG                                       <  SRR4302543.5794402/94‑1 (MQ=60)
                                                          CACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGC                                      >  SRR4302543.7029318/1‑94 (MQ=60)
                                                          CACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGC                                      <  SRR4302543.6594845/94‑1 (MQ=60)
                                                          CACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGC                                      >  SRR4302543.6049466/1‑94 (MQ=60)
                                                           CCAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCT                                     <  SRR4302543.1987612/94‑1 (MQ=60)
                                                           ACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCT                                     >  SRR4302543.5072259/1‑94 (MQ=60)
                                                            CAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTT                                    >  SRR4302543.6906842/1‑94 (MQ=60)
                                                            CAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTT                                    >  SRR4302543.5221930/1‑94 (MQ=60)
                                                            CAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTT                                    >  SRR4302543.3872587/1‑94 (MQ=60)
                                                            CAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTT                                    >  SRR4302543.4270073/1‑94 (MQ=60)
                                                             AACCTGCTGCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTT                                   <  SRR4302543.2349903/94‑1 (MQ=60)
                                                              ACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTG                                  <  SRR4302543.7350264/94‑1 (MQ=60)
                                                              ACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTG                                  <  SRR4302543.4686515/94‑1 (MQ=60)
                                                              ACCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTG                                  >  SRR4302543.4628772/1‑94 (MQ=60)
                                                               CCTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGA                                 <  SRR4302543.3152155/94‑1 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                                >  SRR4302543.6717476/1‑94 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                                <  SRR4302543.4359335/94‑1 (MQ=60)
                                                                CTGCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGAT                                >  SRR4302543.6152390/1‑94 (MQ=60)
                                                                  GCGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGT                              <  SRR4302543.7670692/94‑1 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                             >  SRR4302543.2007794/1‑94 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                             >  SRR4302543.673954/1‑94 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                             >  SRR4302543.3493428/1‑94 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                             >  SRR4302543.4052518/1‑94 (MQ=60)
                                                                   CGTCTATCCACTGCGCCAGGTTTTC‑TGGCGGCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTA                             >  SRR4302543.394224/1‑94 (MQ=60)
                                                                    GTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                            <  SRR4302543.1640323/94‑1 (MQ=60)
                                                                    GTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                            >  SRR4302543.4745393/1‑94 (MQ=60)
                                                                    GTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                            <  SRR4302543.3857372/94‑1 (MQ=60)
                                                                    GTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                            <  SRR4302543.3609221/94‑1 (MQ=60)
                                                                    GTCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTAT                            >  SRR4302543.7564320/1‑94 (MQ=60)
                                                                     TCTATCCACTGCGCGCGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCTATGGCAATTCCGCTTTTAACGCTTTGATGTATT                           <  SRR4302543.1372809/94‑1 (MQ=60)
                                                                     TCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGTCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATT                           <  SRR4302543.6729543/94‑1 (MQ=60)
                                                                     TCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATT                           >  SRR4302543.891038/1‑94 (MQ=60)
                                                                     TCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATT                           <  SRR4302543.7368498/94‑1 (MQ=60)
                                                                     TCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATT                           <  SRR4302543.994869/94‑1 (MQ=60)
                                                                     TCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATT                           >  SRR4302543.3346903/1‑94 (MQ=60)
                                                                     TCTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATT                           <  SRR4302543.1594265/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                          <  SRR4302543.4235003/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                          <  SRR4302543.2086528/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                          <  SRR4302543.3808747/94‑1 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                          >  SRR4302543.5590524/1‑94 (MQ=60)
                                                                      CTATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTG                          >  SRR4302543.5505487/1‑94 (MQ=60)
                                                                       TATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGC                         <  SRR4302543.6504814/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        >  SRR4302543.5625284/1‑94 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        >  SRR4302543.4914350/1‑94 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        <  SRR4302543.7955823/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        >  SRR4302543.3581244/1‑94 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        >  SRR4302543.6775051/1‑94 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        <  SRR4302543.6172426/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        <  SRR4302543.6590409/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        <  SRR4302543.6213431/94‑1 (MQ=60)
                                                                        ATCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCG                        >  SRR4302543.6436892/1‑94 (MQ=60)
                                                                         TCCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGG                       >  SRR4302543.7874239/1‑94 (MQ=60)
                                                                          CCACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGA                      >  SRR4302543.5401361/1‑94 (MQ=60)
                                                                           CACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGAC                     <  SRR4302543.7709580/94‑1 (MQ=60)
                                                                           CACTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGAC                     <  SRR4302543.7709579/94‑1 (MQ=60)
                                                                             CTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCA                   <  SRR4302543.5014335/94‑1 (MQ=60)
                                                                             CTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCA                   >  SRR4302543.3517255/1‑94 (MQ=60)
                                                                             CTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCA                   <  SRR4302543.4378026/94‑1 (MQ=60)
                                                                             CTGCGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCA                   >  SRR4302543.7286964/1‑94 (MQ=60)
                                                                               GCGCGAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAA                 <  SRR4302543.972253/94‑1 (MQ=60)
                                                                                CGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAA                >  SRR4302543.6445103/1‑94 (MQ=60)
                                                                                CGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAA                >  SRR4302543.7966336/1‑94 (MQ=60)
                                                                                CGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAA                <  SRR4302543.4499907/94‑1 (MQ=60)
                                                                                CGCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAA                >  SRR4302543.5088908/1‑94 (MQ=60)
                                                                                 GCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               <  SRR4302543.1235130/94‑1 (MQ=60)
                                                                                 GCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               >  SRR4302543.1473463/1‑94 (MQ=60)
                                                                                 GCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               >  SRR4302543.6301446/1‑94 (MQ=60)
                                                                                 GCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               <  SRR4302543.1779496/94‑1 (MQ=60)
                                                                                 GCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               >  SRR4302543.572015/1‑94 (MQ=60)
                                                                                 GCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               <  SRR4302543.530798/94‑1 (MQ=60)
                                                                                 CCCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAG               <  SRR4302543.530795/94‑1 (MQ=60)
                                                                                  CCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGC              >  SRR4302543.4180348/1‑94 (MQ=60)
                                                                                  CCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGC              <  SRR4302543.5085287/94‑1 (MQ=60)
                                                                                  CCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGC              >  SRR4302543.7649668/1‑94 (MQ=60)
                                                                                  CCAGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGC              >  SRR4302543.4108807/1‑94 (MQ=60)
                                                                                    AGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCG            >  SRR4302543.1013150/1‑94 (MQ=60)
                                                                                    AGGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCG            <  SRR4302543.881522/94‑1 (MQ=60)
                                                                                     GGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGA           >  SRR4302543.988031/1‑94 (MQ=60)
                                                                                     GGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGA           >  SRR4302543.7873675/1‑94 (MQ=60)
                                                                                     GGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGA           <  SRR4302543.4405922/94‑1 (MQ=60)
                                                                                     GGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGA           >  SRR4302543.1781925/1‑94 (MQ=60)
                                                                                     GGTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACAAAAAGCCGA           >  SRR4302543.237138/1‑94 (MQ=60)
                                                                                      GTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAT          <  SRR4302543.4678132/94‑1 (MQ=60)
                                                                                      GTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAT          <  SRR4302543.1773299/94‑1 (MQ=60)
                                                                                      GTTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGAT          <  SRR4302543.7598486/94‑1 (MQ=60)
                                                                                       TTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG         <  SRR4302543.2129445/94‑1 (MQ=60)
                                                                                       TTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG         >  SRR4302543.3666916/1‑94 (MQ=60)
                                                                                       TTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG         <  SRR4302543.2701559/94‑1 (MQ=60)
                                                                                       TTTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATG         <  SRR4302543.4840269/94‑1 (MQ=60)
                                                                                        TTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGA        <  SRR4302543.3646553/94‑1 (MQ=60)
                                                                                        TTTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGA        <  SRR4302543.3218979/94‑1 (MQ=60)
                                                                                         TTC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGAC       <  SRR4302543.3556641/94‑1 (MQ=60)
                                                                                          TC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACG      <  SRR4302543.5608737/94‑1 (MQ=60)
                                                                                          TC‑TGGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACG      >  SRR4302543.6627830/1‑94 (MQ=60)
                                                                                             tgGCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGA    >  SRR4302543.7309085/3‑94 (MQ=60)
                                                                                             G‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAA   <  SRR4302543.949790/94‑1 (MQ=60)
                                                                                               GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAAA  <  SRR4302543.910722/94‑1 (MQ=60)
                                                                                               |                                                                                             
GCGGTGCGCTCTACGGATTGCCCGGCGCGATAGAGATCGCTGCCTAAGCCCGCCCCTGCACAACCTGCGTCTATCCACTGCGCCAGGTTTTC‑T‑GCGTCACGCCGCCAACGGCAAAGACTGCGATGTCCGATGGCAATACCGCTTTTAACGCTTTGATGTATTGCGGACCAAAAGCCGATGACGGAAA  >  NC_000913/3870672‑3870858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑