Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,912,542 T→C D96G (GAT→GGT)  glmU ← N‑acetyl glucosamine‑1‑phosphate uridyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,912,5420TC99.7% 1156.1 / ‑5.2 365D96G (GAT→GGT) glmUN‑acetyl glucosamine‑1‑phosphate uridyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (211/152);  total (212/153)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 391 total aligned reads displayed.

ATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTC  >  NC_000913/3912452‑3912631
                                                                                          |                                                                                          
aTGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTtcacca                                                                                          <  1:1071425/93‑1 (MQ=255)
  gCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCaccatc                                                                                        <  1:4229160/93‑1 (MQ=255)
  gCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCaccatc                                                                                        >  2:1027083/1‑93 (MQ=255)
  gCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCaccatc                                                                                        <  2:1649687/93‑1 (MQ=255)
     aCCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGc                                                                                     >  1:3433194/1‑93 (MQ=255)
      cccTGCGGTTTAGCATCACGCAGACGCTGGAGGGTTTCGACAGAGATCAGCGGCACGCCGCCGTAGAGCATTAAAATGTCTTCACCACCGGCa                                                                                    >  1:6750481/1‑93 (MQ=255)
         tGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCaaag                                                                                 <  1:3822994/93‑1 (MQ=255)
         tGCGGTTTAGCATCACGCAGACGCTGGAGGGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCaaag                                                                                 >  1:5166138/1‑93 (MQ=255)
          gCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCaaaga                                                                                <  1:6820655/93‑1 (MQ=255)
           cGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCaaagaa                                                                               <  1:3700820/93‑1 (MQ=255)
             gTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCaaagaaag                                                                             >  1:6074692/1‑93 (MQ=255)
             gTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCaaagaaag                                                                             <  1:233821/93‑1 (MQ=255)
               ttAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGt                                                                           <  2:6324153/93‑1 (MQ=255)
                 aGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGc                                                                         <  1:4531609/93‑1 (MQ=255)
                  gCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCg                                                                        >  2:6052213/1‑93 (MQ=255)
                     tCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGcc                                                                     >  1:596856/1‑93 (MQ=255)
                      cACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCct                                                                    <  2:553393/93‑1 (MQ=255)
                      cACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCct                                                                    <  1:4137185/93‑1 (MQ=255)
                      cACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCct                                                                    >  2:1307041/1‑93 (MQ=255)
                       aCGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCctg                                                                   >  2:688037/1‑93 (MQ=255)
                        cGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCctgc                                                                  <  2:1845250/93‑1 (MQ=255)
                           aGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCtgctgc                                                               <  1:2754186/93‑1 (MQ=255)
                            gACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca                                                              >  2:6245112/1‑93 (MQ=255)
                            gACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca                                                              >  2:2793931/1‑93 (MQ=255)
                            gACGCTGGAGTGTTTCGACAGAGATCAGAGACACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca                                                              <  2:5849675/93‑1 (MQ=255)
                               gCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca‑ttg                                                          >  1:1462091/1‑93 (MQ=255)
                                 tGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca‑ttgca                                                        >  1:7038430/1‑93 (MQ=255)
                                 tGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca‑ttgca                                                        >  1:3208369/1‑93 (MQ=255)
                                  ggAGTGTTTCGTCCGAGATCATCGGCACGCCGCCGGAGAGCATTAAAATGTATTCACAATCGGCAAAGCAAGGTGCGGCCTGCcgca‑atgtat                                                       >  2:5334505/1‑93 (MQ=255)
                                  ggAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCtgca‑ttgcat                                                       >  1:737307/1‑93 (MQ=255)
                                   gAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATg                                                      >  2:4061706/1‑93 (MQ=255)
                                      tgtTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGAcc                                                   <  2:2901884/93‑1 (MQ=255)
                                       gtTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGAccc                                                  >  2:7189547/1‑93 (MQ=255)
                                        tttCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCg                                                 >  2:2047634/1‑93 (MQ=255)
                                        tttCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCg                                                 >  2:3843179/1‑93 (MQ=255)
                                           cGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTAc                                              <  2:2176366/93‑1 (MQ=255)
                                             aCAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTAccc                                            >  1:2523086/1‑93 (MQ=255)
                                              cAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCa                                           <  1:5404603/93‑1 (MQ=255)
                                                gagaTCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAgc                                         >  1:3083983/1‑93 (MQ=255)
                                                gagaTCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAgc                                         >  1:2811540/1‑93 (MQ=255)
                                                 agaTCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAgct                                        <  1:4667194/93‑1 (MQ=255)
                                                   aTCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACACGTACCCAgctgc                                      >  1:1943179/1‑93 (MQ=255)
                                                    tCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAgctgct                                     >  2:4654849/1‑93 (MQ=255)
                                                     cAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGctc                                    >  2:3040071/1‑93 (MQ=255)
                                                       gCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTTCA‑TTGCATGACCCGTACCCAGCTGCTCTg                                  >  2:1750019/1‑93 (MQ=255)
                                                       gCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGctctc                                  >  1:4287622/1‑92 (MQ=255)
                                                       gCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTg                                  >  1:7910406/1‑93 (MQ=255)
                                                       gCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTg                                  >  1:5129911/1‑93 (MQ=255)
                                                         ggCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGcc                                >  1:4897596/1‑93 (MQ=255)
                                                         ggCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGcc                                >  2:4439458/1‑93 (MQ=255)
                                                         ggCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGcc                                <  1:1539913/93‑1 (MQ=255)
                                                             cGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGaa                            <  2:861277/93‑1 (MQ=255)
                                                             cGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGaa                            >  2:4530263/1‑93 (MQ=255)
                                                                cGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCa                         >  1:3029028/1‑93 (MQ=255)
                                                                cGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCa                         <  1:1363928/93‑1 (MQ=255)
                                                                  ccGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCAcc                       >  1:1777568/1‑93 (MQ=255)
                                                                   cGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCAccc                      >  2:5803051/1‑93 (MQ=255)
                                                                    gTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                     <  2:5546465/93‑1 (MQ=255)
                                                                    gTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                     >  1:5770698/1‑93 (MQ=255)
                                                                    gTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                     >  2:6753945/1‑93 (MQ=255)
                                                                    gTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                     >  1:4584430/1‑93 (MQ=255)
                                                                    gTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCa                     >  1:7282494/1‑93 (MQ=255)
                                                                    gTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TCGCATGACCCGTACCCACCTGCTCCGCCTGAAGCACCCa                     >  1:25992/1‑93 (MQ=255)
                                                                     tAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAg                    >  2:5173555/1‑93 (MQ=255)
                                                                      agagCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGt                   >  2:1195308/1‑93 (MQ=255)
                                                                        agCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTa                 >  2:2654724/1‑93 (MQ=255)
                                                                         gCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTaa                >  1:7468339/1‑93 (MQ=255)
                                                                         gCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTaa                >  2:4917559/1‑93 (MQ=255)
                                                                         gCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTaa                >  2:3324059/1‑93 (MQ=255)
                                                                         gCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTaa                <  1:5319367/93‑1 (MQ=255)
                                                                          cATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAg               <  2:3480189/93‑1 (MQ=255)
                                                                          cATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAg               <  1:5907437/93‑1 (MQ=255)
                                                                           aTTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAgg              <  2:5683689/93‑1 (MQ=255)
                                                                           aTTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAgg              <  2:7898389/93‑1 (MQ=255)
                                                                           aTTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAgg              >  1:103236/1‑93 (MQ=255)
                                                                            ttAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGt             <  2:2763138/93‑1 (MQ=255)
                                                                            ttAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGt             >  2:6553867/1‑93 (MQ=255)
                                                                            ttAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGt             <  1:2127128/93‑1 (MQ=255)
                                                                            ttAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGCACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGt             >  1:92574/1‑93 (MQ=255)
                                                                             tAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGGACCCAGTTAAGGtt            >  1:4968211/1‑93 (MQ=255)
                                                                               aaaTGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgt          >  1:6682719/1‑93 (MQ=255)
                                                                                aaTGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtc         <  1:1168112/93‑1 (MQ=255)
                                                                                aaTGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtc         <  2:732196/93‑1 (MQ=255)
                                                                                aaTGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtc         <  2:1675366/93‑1 (MQ=255)
                                                                                aaTGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtc         >  1:3929933/1‑93 (MQ=255)
                                                                                  tGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgt       <  1:7122883/93‑1 (MQ=255)
                                                                                  tGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgt       >  2:5934831/1‑93 (MQ=255)
                                                                                  tGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgt       >  1:3530425/1‑93 (MQ=255)
                                                                                  tGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgt       >  2:2419496/1‑93 (MQ=255)
                                                                                   gTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgtc      <  2:2981795/93‑1 (MQ=255)
                                                                                   gTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTgtcgtc      >  2:3565770/1‑93 (MQ=255)
                                                                                    tCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCt     >  2:4811872/1‑93 (MQ=255)
                                                                                     cTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCtt    >  1:6204113/1‑93 (MQ=255)
                                                                                     cTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCtt    >  1:6367196/1‑93 (MQ=255)
                                                                                     cTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCtt    >  1:4337048/1‑93 (MQ=255)
                                                                                      ttCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCttt   <  1:7802929/93‑1 (MQ=255)
                                                                                      ttCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCttt   <  2:4216977/93‑1 (MQ=255)
                                                                                       tcaccaTCGGCAAAGAAAGGTGCGGCCTGCTGCATTTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCttt   >  2:61606/1‑93 (MQ=255)
                                                                                       tcaccaTCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTc  <  2:7645097/93‑1 (MQ=255)
                                                                                       tcaccaTCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTc  >  2:2629959/1‑93 (MQ=255)
                                                                                          |                                                                                          
ATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCA‑TTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTC  >  NC_000913/3912452‑3912631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 218 total aligned reads displayed.
CCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCG  >  NC_000913/3912449‑3912635
                                                                                             |                                                                                             
CCAATGCCACCCTGCGGTTTAGCATCACGCAGCCGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCAC                                                                                               <  SRR4302543.6090453/94‑1 (MQ=60)
CCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCAC                                                                                               <  SRR4302543.131253/94‑1 (MQ=60)
 CAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACC                                                                                              >  SRR4302543.4149816/1‑94 (MQ=60)
 CAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACC                                                                                              <  SRR4302543.3367527/94‑1 (MQ=60)
   ATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCAT                                                                                            <  SRR4302543.1081058/94‑1 (MQ=60)
   ATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCAT                                                                                            <  SRR4302543.1081059/94‑1 (MQ=60)
   ATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCAT                                                                                            <  SRR4302543.6663445/94‑1 (MQ=60)
   ATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCAT                                                                                            <  SRR4302543.2427284/94‑1 (MQ=60)
     GCCCCCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCG                                                                                          <  SRR4302543.2753861/94‑1 (MQ=60)
     GCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCG                                                                                          <  SRR4302543.4267523/94‑1 (MQ=60)
     GCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCG                                                                                          <  SRR4302543.4361300/94‑1 (MQ=60)
     GCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCG                                                                                          >  SRR4302543.6188465/1‑94 (MQ=60)
       CACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGC                                                                                        >  SRR4302543.7227708/1‑94 (MQ=60)
       CACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGC                                                                                        >  SRR4302543.6223942/1‑94 (MQ=60)
       CACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGC                                                                                        >  SRR4302543.1887522/1‑94 (MQ=60)
       CACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGC                                                                                        >  SRR4302543.26028/1‑94 (MQ=60)
       CACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGC                                                                                        >  SRR4302543.3463917/1‑94 (MQ=60)
       CACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGC                                                                                        >  SRR4302543.1116359/1‑94 (MQ=60)
        CCCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCA                                                                                       <  SRR4302543.950394/94‑1 (MQ=60)
        ACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCA                                                                                       >  SRR4302543.7021391/1‑94 (MQ=60)
        ACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCA                                                                                       >  SRR4302543.6887012/1‑94 (MQ=60)
        ACCCTGCGGTTTAGCATCACGCAGACGCTGGAGGGTTTCGACAGAGATCAGCGGCACGCCGCCGTAGAGCATTAAAATGTCTTCACCACCGGCA                                                                                       >  SRR4302543.6814230/1‑94 (MQ=60)
          CCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAA                                                                                     <  SRR4302543.6770247/94‑1 (MQ=60)
           CTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAG                                                                                    >  SRR4302543.2501271/1‑94 (MQ=60)
           CTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAG                                                                                    <  SRR4302543.2392092/94‑1 (MQ=60)
           CTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAA                                                                                    >  SRR4302543.2163546/1‑94 (MQ=60)
           CTGCGGTTTAGCATCACGCAGACGCTGGAGGGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAG                                                                                    >  SRR4302543.5212812/1‑94 (MQ=60)
            TGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGA                                                                                   <  SRR4302543.3856713/94‑1 (MQ=60)
             GCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAA                                                                                  <  SRR4302543.3586179/94‑1 (MQ=60)
              CGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAA                                                                                 <  SRR4302543.3733352/94‑1 (MQ=60)
               GGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAG                                                                                >  SRR4302543.6132267/1‑94 (MQ=60)
               GGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAG                                                                                >  SRR4302543.4564246/1‑94 (MQ=60)
               GGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAG                                                                                >  SRR4302543.1220422/1‑94 (MQ=60)
               GGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAG                                                                                >  SRR4302543.6635449/1‑94 (MQ=60)
               GGTTTAGCATCACGCAGACGCCGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAG                                                                                <  SRR4302543.7609926/94‑1 (MQ=60)
                GTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGG                                                                               <  SRR4302543.235840/94‑1 (MQ=60)
                 TTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGT                                                                              <  SRR4302543.1933787/94‑1 (MQ=60)
                 TTTAGCATCACGCAGACGCTGGAGTGTTGCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGT                                                                              <  SRR4302543.4278418/94‑1 (MQ=60)
                  TTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTG                                                                             <  SRR4302543.377245/94‑1 (MQ=60)
                  TTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTG                                                                             <  SRR4302543.3235674/94‑1 (MQ=60)
                  TTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTG                                                                             <  SRR4302543.156608/94‑1 (MQ=60)
                   TAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGC                                                                            <  SRR4302543.4302251/94‑1 (MQ=60)
                    AGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCG                                                                           <  SRR4302543.5923620/94‑1 (MQ=60)
                    AGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCG                                                                           >  SRR4302543.4665587/1‑94 (MQ=60)
                    AGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCG                                                                           <  SRR4302543.3284778/94‑1 (MQ=60)
                    AGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCG                                                                           <  SRR4302543.4572850/94‑1 (MQ=60)
                     GCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGG                                                                          >  SRR4302543.2953692/1‑94 (MQ=60)
                     GCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGG                                                                          <  SRR4302543.2952929/94‑1 (MQ=60)
                       ATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCC                                                                        >  SRR4302543.5986024/1‑94 (MQ=60)
                       ATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCC                                                                        >  SRR4302543.4966703/1‑94 (MQ=60)
                       ATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCC                                                                        >  SRR4302543.602328/1‑94 (MQ=60)
                        TCCCGCCGACGCTGGTGTGTTTCGGCGGCGATCCGCGGCACGTCGCCGTAGAGCATTAAACTGTCTTCACCATCGGCAAAGAGAGGTGCGGCCT                                                                       <  SRR4302543.7116730/94‑1 (MQ=48)
                        TCACGCAGACGCTGGAGTGTCGCGACAGAGATCAGCGTCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCT                                                                       <  SRR4302543.4365618/94‑1 (MQ=60)
                         CACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTG                                                                      >  SRR4302543.2839851/1‑94 (MQ=60)
                         CACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTG                                                                      <  SRR4302543.4174535/94‑1 (MQ=60)
                         CACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTG                                                                      <  SRR4302543.4174537/94‑1 (MQ=60)
                          ACCCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGC                                                                     >  SRR4302543.7431890/1‑94 (MQ=60)
                           CGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCT                                                                    <  SRR4302543.234963/94‑1 (MQ=60)
                           CGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCT                                                                    <  SRR4302543.6160811/94‑1 (MQ=60)
                            GCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTG                                                                   <  SRR4302543.6806092/94‑1 (MQ=60)
                              AGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA                                                                 >  SRR4302543.4386519/1‑94 (MQ=60)
                              AGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA                                                                 >  SRR4302543.2813811/1‑94 (MQ=60)
                              AGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCA                                                                 <  SRR4302543.2779241/94‑1 (MQ=60)
                               GACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCAT                                                                <  SRR4302543.7307266/94‑1 (MQ=60)
                                 CGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTG                                                              >  SRR4302543.3160276/1‑94 (MQ=60)
                                 CGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTG                                                              >  SRR4302543.1475274/1‑94 (MQ=60)
                                   CTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCA                                                            >  SRR4302543.3237325/1‑94 (MQ=60)
                                   CTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCA                                                            >  SRR4302543.7106300/1‑94 (MQ=60)
                                    TGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCAT                                                           <  SRR4302543.7311734/94‑1 (MQ=60)
                                    TGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCAT                                                           >  SRR4302543.743906/1‑94 (MQ=60)
                                    TGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCAT                                                           <  SRR4302543.1488214/94‑1 (MQ=60)
                                    TGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCAT                                                           >  SRR4302543.2088134/1‑94 (MQ=60)
                                    TCGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAACGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCAT                                                           <  SRR4302543.2067778/94‑1 (MQ=60)
                                     GGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATG                                                          >  SRR4302543.4658267/1‑94 (MQ=60)
                                     GGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATG                                                          >  SRR4302543.965074/1‑94 (MQ=60)
                                     GGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATG                                                          >  SRR4302543.3799180/1‑94 (MQ=60)
                                      GAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGA                                                         <  SRR4302543.5205916/94‑1 (MQ=60)
                                      GAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGA                                                         >  SRR4302543.1077107/1‑94 (MQ=60)
                                       AGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGAC                                                        >  SRR4302543.5389167/1‑94 (MQ=60)
                                       AGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGAC                                                        <  SRR4302543.146285/94‑1 (MQ=60)
                                        GTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACC                                                       <  SRR4302543.1547137/94‑1 (MQ=60)
                                        GTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACC                                                       >  SRR4302543.3949439/1‑94 (MQ=60)
                                        GTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACC                                                       <  SRR4302543.2788597/94‑1 (MQ=60)
                                         TGTTTCGACAGAGATCAGCGGCCCGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCC                                                      <  SRR4302543.2783739/94‑1 (MQ=60)
                                         TGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCC                                                      <  SRR4302543.6337603/94‑1 (MQ=60)
                                         TGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCC                                                      <  SRR4302543.810373/94‑1 (MQ=60)
                                          GTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCG                                                     >  SRR4302543.4584788/1‑94 (MQ=60)
                                           TTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGT                                                    >  SRR4302543.5433985/1‑94 (MQ=60)
                                            TTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTA                                                   >  SRR4302543.7946452/1‑94 (MQ=60)
                                            TTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTA                                                   >  SRR4302543.5008658/1‑94 (MQ=60)
                                             TCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTAC                                                  <  SRR4302543.6777886/94‑1 (MQ=60)
                                              CGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACC                                                 <  SRR4302543.5580852/94‑1 (MQ=60)
                                               GACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCC                                                >  SRR4302543.2546027/1‑94 (MQ=60)
                                               GACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCC                                                >  SRR4302543.4589216/1‑94 (MQ=60)
                                                ACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCA                                               <  SRR4302543.7536242/94‑1 (MQ=60)
                                                 CAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAG                                              >  SRR4302543.328186/1‑94 (MQ=60)
                                                 CAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAG                                              <  SRR4302543.5453736/94‑1 (MQ=60)
                                                 CAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAG                                              <  SRR4302543.7424530/94‑1 (MQ=60)
                                                  AGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGC                                             <  SRR4302543.6665874/94‑1 (MQ=60)
                                                  AGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGC                                             >  SRR4302543.2837283/1‑94 (MQ=60)
                                                  AGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGC                                             >  SRR4302543.3111714/1‑94 (MQ=60)
                                                   GAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCT                                            <  SRR4302543.5159295/94‑1 (MQ=60)
                                                   GAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCT                                            <  SRR4302543.6426485/94‑1 (MQ=60)
                                                    AGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTG                                           <  SRR4302543.4709729/94‑1 (MQ=60)
                                                     GATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGC                                          >  SRR4302543.4619104/1‑94 (MQ=60)
                                                     GATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGC                                          >  SRR4302543.6066536/1‑94 (MQ=60)
                                                     GATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACACGTACCCAGCTGC                                          >  SRR4302543.1960633/1‑94 (MQ=60)
                                                      ATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCT                                         >  SRR4302543.3093561/1‑94 (MQ=60)
                                                       TCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTC                                        >  SRR4302543.7130605/1‑94 (MQ=60)
                                                        CAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCT                                       <  SRR4302543.6340888/94‑1 (MQ=60)
                                                         AGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTG                                      >  SRR4302543.7984724/1‑94 (MQ=60)
                                                         AGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTG                                      >  SRR4302543.5176275/1‑94 (MQ=60)
                                                         AGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTC                                      >  SRR4302543.4326590/1‑94 (MQ=60)
                                                           CGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCC                                    <  SRR4302543.3922750/94‑1 (MQ=60)
                                                           CGGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCC                                    >  SRR4302543.4942242/1‑94 (MQ=60)
                                                            GGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCT                                   <  SRR4302543.3085647/94‑1 (MQ=60)
                                                            GGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCT                                   <  SRR4302543.1553705/94‑1 (MQ=60)
                                                            GGCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCT                                   >  SRR4302543.7609116/1‑94 (MQ=60)
                                                             gcccgtccgCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTG                                  <  SRR4302543.5939943/85‑1 (MQ=60)
                                                             GCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTG                                  <  SRR4302543.5939944/94‑1 (MQ=60)
                                                             GCACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTG                                  >  SRR4302543.2704546/1‑94 (MQ=60)
                                                              CACGTCTCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTCCCCAGCTGCTCTGCCTGA                                 <  SRR4302543.1388980/94‑1 (MQ=60)
                                                              CACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGA                                 <  SRR4302543.1397945/94‑1 (MQ=60)
                                                               ACGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAA                                <  SRR4302543.4644510/94‑1 (MQ=60)
                                                                CGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGTTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAG                               >  SRR4302543.4347220/1‑94 (MQ=60)
                                                                CGTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGAGCGGCCGGCTGCATTGCCTGACACGTACCCAGCTGCTCTGCCGGAAG                               >  SRR4302543.490895/1‑94 (MQ=60)
                                                                 GTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGC                              >  SRR4302543.116835/1‑94 (MQ=60)
                                                                 GTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGC                              >  SRR4302543.3222321/1‑94 (MQ=60)
                                                                 GTCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGC                              >  SRR4302543.1049703/1‑94 (MQ=60)
                                                                  TCGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCA                             <  SRR4302543.1636473/94‑1 (MQ=60)
                                                                   CGCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCAC                            <  SRR4302543.497711/94‑1 (MQ=60)
                                                                    GCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACC                           >  SRR4302543.1793448/1‑94 (MQ=60)
                                                                    GCCGTAGAGCATTAAAATGTCTTCACCATCGGCAAACAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACC                           >  SRR4302543.1809393/1‑94 (MQ=60)
                                                                     CCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCC                          <  SRR4302543.865334/94‑1 (MQ=60)
                                                                     CCGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCC                          <  SRR4302543.6017357/94‑1 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCCCCCA                         >  SRR4302543.2338333/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCA                         >  SRR4302543.5823244/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCA                         >  SRR4302543.6103431/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCA                         >  SRR4302543.5179760/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCA                         >  SRR4302543.1243633/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCA                         >  SRR4302543.7352819/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCA                         >  SRR4302543.4626082/1‑94 (MQ=60)
                                                                      CGTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCACGACCCGTACCCAGCTGCTCTGCCTGAAGAACCCA                         >  SRR4302543.4059797/1‑94 (MQ=60)
                                                                       GTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAG                        >  SRR4302543.4016354/1‑94 (MQ=60)
                                                                       GTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAG                        <  SRR4302543.2099339/94‑1 (MQ=60)
                                                                       GTAGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAG                        <  SRR4302543.3716445/94‑1 (MQ=60)
                                                                         AGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTT                      >  SRR4302543.2585558/1‑94 (MQ=60)
                                                                         AGAGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTT                      >  SRR4302543.3489138/1‑94 (MQ=60)
                                                                           AGCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAA                    >  SRR4302543.7539864/1‑94 (MQ=60)
                                                                            GCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAG                   >  SRR4302543.4538397/1‑94 (MQ=60)
                                                                            GCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAG                   <  SRR4302543.5367431/94‑1 (MQ=60)
                                                                            GCATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAG                   <  SRR4302543.5367430/94‑1 (MQ=60)
                                                                             CATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGG                  >  SRR4302543.104191/1‑94 (MQ=60)
                                                                             CATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGG                  <  SRR4302543.686602/94‑1 (MQ=60)
                                                                             CATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGG                  <  SRR4302543.5961358/94‑1 (MQ=60)
                                                                              ATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGT                 >  SRR4302543.3490097/1‑94 (MQ=60)
                                                                              ATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGT                 >  SRR4302543.2611202/1‑94 (MQ=60)
                                                                              ATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGT                 >  SRR4302543.413016/1‑94 (MQ=60)
                                                                              ATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGT                 >  SRR4302543.7953193/1‑94 (MQ=60)
                                                                              ATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGCT                 >  SRR4302543.5171402/1‑94 (MQ=60)
                                                                              ATTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGCACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGT                 >  SRR4302543.93440/1‑94 (MQ=60)
                                                                               TTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGGACCCAGTTAAGGTT                >  SRR4302543.5013432/1‑94 (MQ=60)
                                                                               TTAAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTT                <  SRR4302543.853893/94‑1 (MQ=60)
                                                                                 AAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGT              >  SRR4302543.6745804/1‑94 (MQ=60)
                                                                                 AAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGT              <  SRR4302543.5345159/94‑1 (MQ=60)
                                                                                 AAAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGT              <  SRR4302543.3518269/94‑1 (MQ=60)
                                                                                  AAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTC             >  SRR4302543.6022688/1‑94 (MQ=60)
                                                                                  AAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTC             >  SRR4302543.3964630/1‑94 (MQ=60)
                                                                                  AAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTC             >  SRR4302543.7896825/1‑94 (MQ=60)
                                                                                  AAATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTC             >  SRR4302543.5149965/1‑94 (MQ=60)
                                                                                   AATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCG            <  SRR4302543.5573010/94‑1 (MQ=60)
                                                                                   AATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCG            <  SRR4302543.1178725/94‑1 (MQ=60)
                                                                                    ATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGT           >  SRR4302543.4762794/1‑94 (MQ=60)
                                                                                    ATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGT           >  SRR4302543.6878289/1‑94 (MQ=60)
                                                                                    ATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGT           >  SRR4302543.3907740/1‑94 (MQ=60)
                                                                                    ATGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGT           >  SRR4302543.3561868/1‑94 (MQ=60)
                                                                                     TGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTC          <  SRR4302543.7192078/94‑1 (MQ=60)
                                                                                     TGTCTTCACCATCGGCAAAGCAAGGTACGGCCTGCTGCATTGCATGACACGTACTCACCTGCTCTGCCTGAAGCACCCAGTTAATGTTGTCGTT          >  SRR4302543.7515705/1‑94 (MQ=60)
                                                                                     TGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTC          >  SRR4302543.4277234/1‑94 (MQ=60)
                                                                                     TGTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTC          >  SRR4302543.7515704/1‑94 (MQ=60)
                                                                                      GTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCT         >  SRR4302543.3058082/1‑94 (MQ=60)
                                                                                      GTCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCT         >  SRR4302543.532505/1‑94 (MQ=60)
                                                                                       TCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTT        >  SRR4302543.4376383/1‑94 (MQ=60)
                                                                                       TCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTT        >  SRR4302543.6262699/1‑94 (MQ=60)
                                                                                       TCTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTT        <  SRR4302543.6835974/94‑1 (MQ=60)
                                                                                        CTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTT       >  SRR4302543.1293814/1‑94 (MQ=60)
                                                                                        CTTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTT       <  SRR4302543.7098802/94‑1 (MQ=60)
                                                                                         TTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTC      <  SRR4302543.3620628/94‑1 (MQ=60)
                                                                                         TTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTC      <  SRR4302543.7876483/94‑1 (MQ=60)
                                                                                         TTCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTC      >  SRR4302543.1553040/1‑94 (MQ=60)
                                                                                          TCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCA     >  SRR4302543.7477542/1‑94 (MQ=60)
                                                                                          TCACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCA     <  SRR4302543.5935860/94‑1 (MQ=60)
                                                                                            ACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTGTTTCAGC   >  SRR4302543.1696907/1‑94 (MQ=60)
                                                                                            ACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGC   <  SRR4302543.7664585/94‑1 (MQ=60)
                                                                                            ACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGC   >  SRR4302543.292246/1‑94 (MQ=60)
                                                                                            ACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGC   >  SRR4302543.457427/1‑94 (MQ=60)
                                                                                            ACCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGC   >  SRR4302543.1922095/1‑94 (MQ=60)
                                                                                             CCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCG  <  SRR4302543.1583218/94‑1 (MQ=60)
                                                                                             CCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCG  >  SRR4302543.6124646/1‑94 (MQ=60)
                                                                                             CCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCG  <  SRR4302543.2071886/94‑1 (MQ=60)
                                                                                             |                                                                                             
CCAATGCCACCCTGCGGTTTAGCATCACGCAGACGCTGGAGTGTTTCGACAGAGATCAGCGGCACGTCGCCGTAGAGCATTAAAATGTCTTCATCATCGGCAAAGAAAGGTGCGGCCTGCTGCATTGCATGACCCGTACCCAGCTGCTCTGCCTGAAGCACCCAGTTAAGGTTGTCGTCTTTCAGCG  >  NC_000913/3912449‑3912635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑