Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,918,274 T→C K81K (AAA→AAG)  atpF ← membrane‑bound ATP synthase, F0 sector, subunit b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,2740TC99.8% 1304.4 / ‑5.2 414K81K (AAA→AAG) atpFmembrane‑bound ATP synthase, F0 sector, subunit b
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (237/176);  total (238/176)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 429 total aligned reads displayed.

GGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTT  >  NC_000913/3918184‑3918363
                                                                                          |                                                                                         
agCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGctt                                                                                         >  2:1211564/2‑93 (MQ=255)
 gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTg                                                                                        >  1:5468262/1‑93 (MQ=255)
 gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTg                                                                                        <  1:3207577/93‑1 (MQ=255)
 gCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTg                                                                                        >  2:3436018/1‑93 (MQ=255)
   ttCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGtt                                                                                      <  1:1705742/93‑1 (MQ=255)
    taattttCCGCCTGCGCCTGGGCACTGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTc                                                                                     <  2:4401791/89‑1 (MQ=255)
    tCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTc                                                                                     >  1:6198400/1‑93 (MQ=255)
    tCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTc                                                                                     >  1:1763870/1‑93 (MQ=255)
       aTTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGcc                                                                                  <  1:40140/93‑1 (MQ=255)
        tttCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCt                                                                                 <  1:6962086/93‑1 (MQ=255)
        tttCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCt                                                                                 <  1:3713795/93‑1 (MQ=255)
          tCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGc                                                                               >  1:6827851/1‑93 (MQ=255)
          tCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGc                                                                               >  1:4369672/1‑93 (MQ=255)
           ccGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCt                                                                              <  2:3994202/93‑1 (MQ=255)
            cgcctgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTc                                                                             >  1:587011/1‑93 (MQ=255)
              cctgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCga                                                                           >  1:1266436/1‑93 (MQ=255)
               ctgcgccttGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgat                                                                          <  1:3887547/93‑1 (MQ=255)
               ctgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgat                                                                          >  2:461527/1‑93 (MQ=255)
               ctgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgat                                                                          >  2:2334209/1‑93 (MQ=255)
                tgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgctg                                                                         >  2:3071150/1‑93 (MQ=255)
                tgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatg                                                                         >  1:4813680/1‑93 (MQ=255)
                tgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatg                                                                         >  1:3795311/1‑93 (MQ=255)
                tgcgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCgatg                                                                         <  1:1971811/93‑1 (MQ=255)
                  cgcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTTCTCgatgat                                                                       >  2:4052036/1‑93 (MQ=255)
                   gcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAtt                                                                      >  1:4651715/1‑93 (MQ=255)
                   gcctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAtt                                                                      >  1:2305720/1‑93 (MQ=255)
                     ctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAc                                                                    <  2:6591461/93‑1 (MQ=255)
                     ctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAc                                                                    >  2:5445807/1‑93 (MQ=255)
                     ctgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAc                                                                    <  2:5358161/93‑1 (MQ=255)
                      tgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAcc                                                                   <  2:4735370/93‑1 (MQ=255)
                      tgGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTAcc                                                                   <  1:3452675/93‑1 (MQ=255)
                       gggCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCt                                                                  >  1:580907/1‑93 (MQ=255)
                       gggCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCt                                                                  >  1:7355567/1‑93 (MQ=255)
                       gggCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCt                                                                  <  2:6121225/93‑1 (MQ=255)
                         gCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTgg                                                                >  1:5285107/1‑93 (MQ=255)
                         gCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTgg                                                                >  2:1033753/1‑93 (MQ=255)
                          ccACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTggg                                                               <  1:311279/93‑1 (MQ=255)
                           cACTATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGc                                                              <  2:3510063/93‑1 (MQ=255)
                           cACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGc                                                              >  2:871166/1‑93 (MQ=255)
                            aCGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGGGAGCGGGGCTTGTTCGCCTGCTCGATGATTCCCTGGGCt                                                             >  1:6587340/1‑93 (MQ=255)
                            aCGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCt                                                             >  2:3853131/1‑93 (MQ=255)
                                ttttAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcg                                                         >  1:5037874/1‑93 (MQ=255)
                                 tttAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgc                                                        <  1:6023476/93‑1 (MQ=255)
                                     gTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttc                                                    >  2:4229719/1‑93 (MQ=255)
                                     gTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttc                                                    >  1:566675/1‑93 (MQ=255)
                                      tACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcg                                                   >  2:7565773/1‑93 (MQ=255)
                                      tACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcg                                                   <  1:134950/93‑1 (MQ=255)
                                         gTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgctt                                                >  1:7026471/1‑93 (MQ=255)
                                         gTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgctt                                                >  2:6906741/1‑93 (MQ=255)
                                         gTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgctt                                                >  1:5381240/1‑93 (MQ=255)
                                          ttCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCcgctttcgcttt                                               <  2:6671444/93‑1 (MQ=255)
                                           tCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCtttt                                              <  1:627653/93‑1 (MQ=255)
                                            tcTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCttttt                                             <  1:2465262/92‑1 (MQ=255)
                                            ccTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCttttt                                             >  2:4830555/1‑93 (MQ=255)
                                             cTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCtttttt                                            <  2:6392935/93‑1 (MQ=255)
                                             cTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCtttttt                                            >  2:2415909/1‑93 (MQ=255)
                                              tGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTc                                           >  2:5610136/1‑93 (MQ=255)
                                              tGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTc                                           <  2:4446985/93‑1 (MQ=255)
                                               gTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCa                                          <  2:1807210/93‑1 (MQ=255)
                                                   tGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTg                                      <  2:2077534/93‑1 (MQ=255)
                                                   tGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTg                                      <  1:1630067/93‑1 (MQ=255)
                                                     ccTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggt                                    >  1:7507345/1‑93 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   <  2:5766780/93‑1 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   <  2:7430589/93‑1 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   >  1:7867231/1‑93 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   >  1:274180/1‑93 (MQ=255)
                                                      cTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtc                                   <  2:4912512/93‑1 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCCGCTggtcg                                  >  2:3255165/1‑93 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  <  1:5963209/93‑1 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  >  2:5249765/1‑93 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  >  2:5128266/1‑93 (MQ=255)
                                                       tCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcg                                  >  2:6239387/1‑93 (MQ=255)
                                                          gCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcggtc                               >  2:7860294/1‑93 (MQ=255)
                                                          gCTTTCGCTGCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTggtcggtc                               <  1:7662214/93‑1 (MQ=255)
                                                           cTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGgtcggtcg                              <  1:2124757/93‑1 (MQ=255)
                                                            tttCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTcgc                             >  2:711373/1‑93 (MQ=255)
                                                              tCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCgcgc                           <  2:2988298/93‑1 (MQ=255)
                                                               cGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCt                          >  1:4401060/1‑93 (MQ=255)
                                                               cGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCt                          <  2:7706616/93‑1 (MQ=255)
                                                                 cTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTgg                        <  1:947127/93‑1 (MQ=255)
                                                                 cTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTgg                        >  2:2218858/1‑93 (MQ=255)
                                                                  ttCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGc                       >  2:2704059/1‑93 (MQ=255)
                                                                   tCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGcc                      <  2:3703409/93‑1 (MQ=255)
                                                                   tCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGcc                      <  1:1041263/93‑1 (MQ=255)
                                                                     gTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCtt                    >  1:4961897/1‑93 (MQ=255)
                                                                      tCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCttt                   <  1:2201611/93‑1 (MQ=255)
                                                                      tCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCttt                   >  1:7210357/1‑93 (MQ=255)
                                                                       ccAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCtttc                  >  1:336463/1‑92 (MQ=255)
                                                                       ccAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTg                  >  2:7277071/1‑93 (MQ=255)
                                                                           aaTCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaag              <  2:6798759/93‑1 (MQ=255)
                                                                           aaTCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaag              <  2:7913943/93‑1 (MQ=255)
                                                                           aaTCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaag              <  2:1550615/93‑1 (MQ=255)
                                                                            aTCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaagg             >  2:2918230/1‑93 (MQ=255)
                                                                             tCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggt            <  1:2613620/93‑1 (MQ=255)
                                                                                gCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtcaa         <  1:5150860/93‑1 (MQ=255)
                                                                                 cGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtcaag        <  1:1207532/93‑1 (MQ=255)
                                                                                  gAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGcaaggtcaagg       <  2:1717701/93‑1 (MQ=255)
                                                                                    gcggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCaaggtcaaggtc     >  2:2402658/1‑93 (MQ=255)
                                                                                      ggcgCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCt   <  2:1443061/93‑1 (MQ=255)
                                                                                       gcgcTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCtt  >  1:814283/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTT  >  NC_000913/3918184‑3918363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 234 total aligned reads displayed.
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  >  NC_000913/3918181‑3918367
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CTCAGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGC                                                                                               >  SRR4302543.4690885/1‑94 (MQ=60)
 TCAGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCT                                                                                              <  SRR4302543.6069020/94‑1 (MQ=60)
 TCAGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCT                                                                                              >  SRR4302543.5628237/1‑94 (MQ=60)
  CAGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTT                                                                                             <  SRR4302543.467549/94‑1 (MQ=60)
  CAGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTT                                                                                             >  SRR4302543.1314387/1‑94 (MQ=60)
  CAGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTAGCTTCGTCCAGAATCTGCGAGCGGCGCTT                                                                                             >  SRR4302543.3987212/1‑94 (MQ=60)
   AGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTG                                                                                            >  SRR4302543.4433300/1‑94 (MQ=60)
   AGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTG                                                                                            >  SRR4302543.6908720/1‑94 (MQ=60)
   AGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTG                                                                                            >  SRR4302543.5517798/1‑94 (MQ=60)
   AGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTG                                                                                            >  SRR4302543.388310/1‑94 (MQ=60)
    GCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGT                                                                                           >  SRR4302543.458408/1‑94 (MQ=60)
     CTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTT                                                                                          >  SRR4302543.1295298/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302543.6256946/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302543.7550608/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         <  SRR4302543.1721014/94‑1 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302543.1758772/1‑94 (MQ=60)
      TTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTC                                                                                         >  SRR4302543.1779638/1‑94 (MQ=60)
         AATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCC                                                                                      >  SRR4302543.756602/1‑94 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     >  SRR4302543.4434884/1‑94 (MQ=60)
          ATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCT                                                                                     <  SRR4302543.5877429/94‑1 (MQ=60)
           TTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTG                                                                                    >  SRR4302543.4048838/1‑94 (MQ=60)
           TTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTG                                                                                    <  SRR4302543.7027595/94‑1 (MQ=60)
           TTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTG                                                                                    <  SRR4302543.2422743/94‑1 (MQ=60)
           TTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTG                                                                                    <  SRR4302543.5282476/94‑1 (MQ=60)
           TTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTG                                                                                    <  SRR4302543.5282477/94‑1 (MQ=60)
           TTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTG                                                                                    >  SRR4302543.1307772/1‑94 (MQ=60)
            TTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGC                                                                                   >  SRR4302543.4409250/1‑94 (MQ=60)
            TTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGC                                                                                   >  SRR4302543.6892311/1‑94 (MQ=60)
             TCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCT                                                                                  <  SRR4302543.7135190/94‑1 (MQ=60)
              CCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTC                                                                                 <  SRR4302543.5464019/94‑1 (MQ=60)
              CCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTC                                                                                 >  SRR4302543.6766539/1‑94 (MQ=60)
              CCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTC                                                                                 <  SRR4302543.5464020/94‑1 (MQ=60)
              CCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTC                                                                                 >  SRR4302543.592369/1‑94 (MQ=60)
              CCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTC                                                                                 <  SRR4302543.3262976/94‑1 (MQ=60)
               CGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCG                                                                                <  SRR4302543.1430288/94‑1 (MQ=60)
               CGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCG                                                                                >  SRR4302543.4819276/1‑94 (MQ=60)
                GCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTTGA                                                                               >  SRR4302543.3150602/1‑94 (MQ=60)
                GCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGA                                                                               >  SRR4302543.5876888/1‑94 (MQ=60)
                GCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGA                                                                               >  SRR4302543.1277767/1‑94 (MQ=60)
                 CCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGAT                                                                              >  SRR4302543.2409059/1‑94 (MQ=60)
                 CCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGAT                                                                              >  SRR4302543.5844088/1‑94 (MQ=60)
                 CCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCCGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGAT                                                                              <  SRR4302543.7059542/94‑1 (MQ=60)
                 CCTGCGCCTGGGCCACAATTTTAGTACGTTCCTGTTCCGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGAT                                                                              <  SRR4302543.7059539/94‑1 (MQ=60)
                  CTGCGCCTTGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             <  SRR4302543.3921862/94‑1 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             <  SRR4302543.897490/94‑1 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             <  SRR4302543.1876352/94‑1 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             >  SRR4302543.3828748/1‑94 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             >  SRR4302543.4857637/1‑94 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATG                                                                             >  SRR4302543.3449594/1‑94 (MQ=60)
                  CTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCCCCCGCTCGATG                                                                             >  SRR4302543.5690250/1‑94 (MQ=60)
                   TGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGA                                                                            <  SRR4302543.1989483/94‑1 (MQ=60)
                    GCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAT                                                                           <  SRR4302543.4187415/94‑1 (MQ=60)
                    GCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAT                                                                           <  SRR4302543.1747716/94‑1 (MQ=60)
                    GCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGAT                                                                           <  SRR4302543.6010926/94‑1 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          <  SRR4302543.6164420/94‑1 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          >  SRR4302543.7944839/1‑94 (MQ=60)
                     CGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATT                                                                          >  SRR4302543.2326723/1‑94 (MQ=60)
                      GCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGCTTA                                                                         >  SRR4302543.1104347/1‑94 (MQ=60)
                      GCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTA                                                                         >  SRR4302543.314502/1‑94 (MQ=60)
                      GCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTA                                                                         <  SRR4302543.3707536/94‑1 (MQ=60)
                       CCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGCGCGGCGCTTGTTCGCCTGCTCGATGATTAC                                                                        >  SRR4302543.3412839/1‑94 (MQ=60)
                        CTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACC                                                                       >  SRR4302543.536643/1‑94 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      >  SRR4302543.2211815/1‑94 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      <  SRR4302543.3483592/94‑1 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      <  SRR4302543.942323/94‑1 (MQ=60)
                         TGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCT                                                                      >  SRR4302543.586216/1‑94 (MQ=60)
                          GGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTG                                                                     <  SRR4302543.5458784/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302543.1954292/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302543.2558043/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    >  SRR4302543.5332807/1‑94 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302543.6253656/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302543.2315468/94‑1 (MQ=60)
                           GGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGG                                                                    <  SRR4302543.3536268/94‑1 (MQ=60)
                             CCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGTGC                                                                  >  SRR4302543.462735/1‑94 (MQ=60)
                             CCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGC                                                                  <  SRR4302543.5724954/94‑1 (MQ=60)
                             CCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGC                                                                  >  SRR4302543.6977269/1‑94 (MQ=60)
                             CCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGC                                                                  <  SRR4302543.314085/94‑1 (MQ=60)
                             CCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGC                                                                  >  SRR4302543.4057670/1‑94 (MQ=60)
                              CACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGGGAGCGGGGCTTGTTCGCCTGCTCGATGATTCCCTGGGCT                                                                 >  SRR4302543.6649393/1‑94 (MQ=60)
                                CGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTC                                                               >  SRR4302543.4804215/1‑94 (MQ=60)
                                 GATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCC                                                              >  SRR4302543.281259/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302543.2631654/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302543.5083696/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302543.2319246/1‑94 (MQ=60)
                                  ATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCG                                                             >  SRR4302543.7328723/1‑94 (MQ=60)
                                    TTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCT                                                           <  SRR4302543.6079450/94‑1 (MQ=60)
                                     TTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTT                                                          >  SRR4302543.1271078/1‑94 (MQ=60)
                                     TTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTT                                                          <  SRR4302543.1072993/94‑1 (MQ=60)
                                     TGAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCTGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTT                                                          <  SRR4302543.6300573/94‑1 (MQ=60)
                                      TAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTT                                                         >  SRR4302543.5183746/1‑94 (MQ=60)
                                      TAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTT                                                         >  SRR4302543.1761341/1‑94 (MQ=60)
                                      TAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTT                                                         <  SRR4302543.3756241/94‑1 (MQ=60)
                                       AGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTC                                                        >  SRR4302543.5092098/1‑94 (MQ=60)
                                       AGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTC                                                        >  SRR4302543.571863/1‑94 (MQ=60)
                                        GTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCG                                                       >  SRR4302543.2123990/1‑94 (MQ=60)
                                         TACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGC                                                      <  SRR4302543.136147/94‑1 (MQ=60)
                                         TACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGC                                                      >  SRR4302543.7987459/1‑94 (MQ=60)
                                          TCGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCT                                                     <  SRR4302543.1066813/94‑1 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302543.7093898/1‑94 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302543.6330844/1‑94 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302543.5052350/1‑94 (MQ=60)
                                           CGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTT                                                    >  SRR4302543.5430063/1‑94 (MQ=60)
                                            GTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTT                                                   >  SRR4302543.7321700/1‑94 (MQ=60)
                                            GTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTT                                                   >  SRR4302543.5073153/1‑94 (MQ=60)
                                            GTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTT                                                   <  SRR4302543.414428/94‑1 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  >  SRR4302543.7139590/1‑94 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  >  SRR4302543.2688930/1‑94 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  >  SRR4302543.2688929/1‑94 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  <  SRR4302543.4442923/94‑1 (MQ=60)
                                             TTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTT                                                  <  SRR4302543.4841706/94‑1 (MQ=60)
                                             TTCCTGTTCTACCTCAGCTTTCGCTTCGTCCACAATCTGCGACCGTCGCTTGTTCTCCTGCTCGctgagttccgatgtttccgctttgtttttt                                                  >  SRR4302543.2688676/1‑64 (MQ=32)
                                              TCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTT                                                 <  SRR4302543.633396/94‑1 (MQ=60)
                                              TCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTT                                                 >  SRR4302543.122458/1‑94 (MQ=60)
                                               TCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTT                                                <  SRR4302543.2487501/94‑1 (MQ=60)
                                                  GTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAG                                             <  SRR4302543.5128183/94‑1 (MQ=60)
                                                  GTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAG                                             <  SRR4302543.4004467/94‑1 (MQ=60)
                                                   TTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGC                                            >  SRR4302543.7569959/1‑94 (MQ=60)
                                                     CTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTG                                          <  SRR4302543.4850268/94‑1 (MQ=60)
                                                     CTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTG                                          <  SRR4302543.6677793/94‑1 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         <  SRR4302543.5745403/94‑1 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         <  SRR4302543.3281293/94‑1 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         <  SRR4302543.1644594/94‑1 (MQ=60)
                                                      TGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGG                                         <  SRR4302543.6049253/94‑1 (MQ=60)
                                                       GCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGT                                        >  SRR4302543.820127/1‑94 (MQ=60)
                                                       GCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGT                                        >  SRR4302543.1658633/1‑94 (MQ=60)
                                                        CCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTC                                       >  SRR4302543.7941310/1‑94 (MQ=60)
                                                        CCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTC                                       >  SRR4302543.276596/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302543.35533/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302543.1807133/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302543.912266/1‑94 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      <  SRR4302543.6014136/94‑1 (MQ=60)
                                                         CTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCG                                      >  SRR4302543.392653/1‑94 (MQ=60)
                                                          TCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGG                                     <  SRR4302543.6017812/94‑1 (MQ=60)
                                                           CAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGT                                    <  SRR4302543.3342410/94‑1 (MQ=60)
                                                           CAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGT                                    <  SRR4302543.654918/94‑1 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTG                                   >  SRR4302543.7513966/1‑94 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTC                                   >  SRR4302543.298951/1‑94 (MQ=60)
                                                            AGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTC                                   >  SRR4302543.6258776/1‑94 (MQ=60)
                                                             GCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCG                                  >  SRR4302543.3878063/1‑94 (MQ=60)
                                                             GCTTTCGCTGCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCG                                  <  SRR4302543.7734835/94‑1 (MQ=60)
                                                             GCTTTCGATTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCG                                  <  SRR4302543.5559446/94‑1 (MQ=60)
                                                              CTTTCGTTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGC                                 >  SRR4302543.3763705/1‑94 (MQ=60)
                                                              CTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGC                                 >  SRR4302543.3763706/1‑94 (MQ=60)
                                                              CTTTCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGC                                 <  SRR4302543.2143780/94‑1 (MQ=60)
                                                                 TCGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCT                              >  SRR4302543.4440955/1‑94 (MQ=60)
                                                                  CGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTG                             >  SRR4302543.7878292/1‑94 (MQ=60)
                                                                  CGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTG                             >  SRR4302543.7697378/1‑94 (MQ=60)
                                                                  CGCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTG                             >  SRR4302543.959510/1‑94 (MQ=60)
                                                                   GCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGG                            >  SRR4302543.1404483/1‑94 (MQ=60)
                                                                   GCTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGG                            >  SRR4302543.7788003/1‑94 (MQ=60)
                                                                    CTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGC                           <  SRR4302543.955401/94‑1 (MQ=60)
                                                                    CTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGC                           >  SRR4302543.1569279/1‑94 (MQ=60)
                                                                    CTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGC                           >  SRR4302543.2277122/1‑94 (MQ=60)
                                                                    CTTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGC                           >  SRR4302543.3198377/1‑94 (MQ=60)
                                                                     TTCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCC                          <  SRR4302543.2890002/94‑1 (MQ=60)
                                                                      TCGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCT                         <  SRR4302543.1050541/94‑1 (MQ=60)
                                                                       CGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTT                        >  SRR4302543.5007065/1‑94 (MQ=60)
                                                                       CGTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTT                        >  SRR4302543.5007066/1‑94 (MQ=60)
                                                                        GTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTT                       >  SRR4302543.7280257/1‑94 (MQ=60)
                                                                        GTCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTT                       >  SRR4302543.7280255/1‑94 (MQ=60)
                                                                         TCCAGACTCTGCGAGCGGCGCTTGTTCGCCTTCGCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTG                      <  SRR4302543.2221424/94‑1 (MQ=60)
                                                                         TCCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTC                      >  SRR4302543.339554/1‑94 (MQ=60)
                                                                          CCAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGC                     >  SRR4302543.5643623/1‑94 (MQ=60)
                                                                           CAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCA                    <  SRR4302543.2203244/94‑1 (MQ=60)
                                                                           CAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCA                    <  SRR4302543.4884643/94‑1 (MQ=60)
                                                                           CAGAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCA                    >  SRR4302543.2542780/1‑94 (MQ=60)
                                                                             GAATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAG                  >  SRR4302543.3532089/1‑94 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAATG                 >  SRR4302543.5385043/1‑94 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 <  SRR4302543.5978756/94‑1 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 >  SRR4302543.6600446/1‑94 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 <  SRR4302543.7635031/94‑1 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 >  SRR4302543.245106/1‑94 (MQ=60)
                                                                              AATCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGG                 >  SRR4302543.5945578/1‑94 (MQ=60)
                                                                                TCTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTC               <  SRR4302543.2637327/94‑1 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              >  SRR4302543.5205061/1‑94 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGGCA              >  SRR4302543.66937/1‑94 (MQ=60)
                                                                                 CTGCGAGCGGCGCTTGTACGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCA              <  SRR4302543.7084694/94‑1 (MQ=60)
                                                                                  TGCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAA             >  SRR4302543.5568986/1‑94 (MQ=60)
                                                                                   GCGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAG            <  SRR4302543.1645597/94‑1 (MQ=60)
                                                                                    CGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGG           <  SRR4302543.1218394/94‑1 (MQ=60)
                                                                                    CGAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGG           >  SRR4302543.5055070/1‑94 (MQ=60)
                                                                                     GAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGT          <  SRR4302543.7611672/94‑1 (MQ=60)
                                                                                     GAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGT          <  SRR4302543.2040957/94‑1 (MQ=60)
                                                                                     GAGCGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGT          <  SRR4302543.5142656/94‑1 (MQ=60)
                                                                                        CGGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCT       <  SRR4302543.6066157/94‑1 (MQ=60)
                                                                                         GGCGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTT      >  SRR4302543.821454/1‑94 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    <  SRR4302543.6284248/94‑1 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    <  SRR4302543.199412/94‑1 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    >  SRR4302543.6051490/1‑94 (MQ=60)
                                                                                           CGCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTAT    <  SRR4302543.7825081/94‑1 (MQ=60)
                                                                                            GCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATG   <  SRR4302543.2207236/94‑1 (MQ=60)
                                                                                            GCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATG   <  SRR4302543.7492882/94‑1 (MQ=60)
                                                                                            GCTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATG   >  SRR4302543.3796841/1‑94 (MQ=60)
                                                                                             TTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCTGTCGCGCTGGCCTTTGCAAGGGCCAGGTCCTTATGT  >  SRR4302543.7558344/1‑94 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  <  SRR4302543.6427629/94‑1 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  >  SRR4302543.6575513/1‑94 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  >  SRR4302543.1447383/1‑94 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  <  SRR4302543.3639813/94‑1 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  <  SRR4302543.5248967/94‑1 (MQ=60)
                                                                                             CTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  <  SRR4302543.505703/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTCGGCTTCAATTTCCGCCTGCGCCTGGGCCACGATTTTAGTACGTTCCTGTTCTGCCTCAGCTTTCGCTTCGTCCAGAATCTGCGAGCGGCGTTTGTTCGCCTGCTCGATGATTACCTGGGCTTCCGCTTTCGCTTTTTTCAGCTGGTCGGTCGCGCTGGCCTTTGCAAGGTCAAGGTCCTTATGT  >  NC_000913/3918181‑3918367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑