Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,987,586 2 bp→GC coding (814‑815/963 nt) hemC ← porphobilinogen deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,987,5860CG100.0% 1009.4 / NA 325R272A (CGG→GCG) hemCporphobilinogen deaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (166/159);  total (166/159)
*NC_0009133,987,5870GC99.4% 1004.9 / ‑3.0 324R272A (CGG→GCG) hemCporphobilinogen deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (165/157);  minor base A (0/2);  total (165/159)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01

Only 100 of 343 total aligned reads displayed.

GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987500‑3987673
                                                                                      ||                                                                                      
gCTCTTCTGCCAGCGACATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGa                                                                                   >  2:6807384/1‑93 (MQ=255)
 ctctTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAc                                                                                  <  2:6557780/93‑1 (MQ=255)
 ctctTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAc                                                                                  >  1:4335668/1‑93 (MQ=255)
  tcttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAcc                                                                                 >  2:5921993/1‑93 (MQ=255)
   cttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCa                                                                                >  1:2881002/1‑93 (MQ=255)
    ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               >  1:6421883/1‑93 (MQ=255)
    ttctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAg                                                                               >  2:7182382/1‑93 (MQ=255)
     tctGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAgc                                                                              <  1:2164441/93‑1 (MQ=255)
       tGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACAGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAGCAgagc                                                                            >  2:4361717/1‑93 (MQ=255)
          cAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacg                                                                         >  2:6839499/1‑93 (MQ=255)
            gCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGcgcacgca                                                                       <  2:2653858/93‑1 (MQ=255)
              gAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGc                                                                     >  1:3044147/1‑93 (MQ=255)
               aaaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGcc                                                                    >  2:6731355/1‑93 (MQ=255)
                aaTCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCa                                                                   <  2:2861107/93‑1 (MQ=255)
                  tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  1:5805590/1‑93 (MQ=255)
                  tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 <  1:7891702/93‑1 (MQ=255)
                  tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 >  1:3367004/1‑93 (MQ=255)
                  tCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGa                                                                 <  2:5407448/93‑1 (MQ=255)
                   ccccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAt                                                                <  1:386936/93‑1 (MQ=255)
                    cccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                               <  2:3838882/93‑1 (MQ=255)
                    cccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtt                                                               <  2:7567222/93‑1 (MQ=255)
                     ccATTTGTTCGGCATCTTGCGGCGCACCGCGGCGATCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAttt                                                              >  2:1735949/1‑93 (MQ=255)
                      cATTTGTTGGGCAGTTTGCGGCGCAGCGCGCCCTTCCCCGCGAATAATCTGCGAACCCTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             <  1:964035/93‑1 (MQ=255)
                      cATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAtctc                                                             >  2:6527018/1‑93 (MQ=255)
                      cATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTc                                                             <  2:603504/93‑1 (MQ=255)
                       aTTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCg                                                            >  1:165919/1‑93 (MQ=255)
                        tttGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGc                                                           <  2:6622756/93‑1 (MQ=255)
                           gTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAt                                                        >  1:861548/1‑93 (MQ=255)
                            ttCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                       >  1:7144639/1‑93 (MQ=255)
                            ttCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                       >  2:7894152/1‑93 (MQ=255)
                            ttCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                       >  1:1858332/1‑93 (MQ=255)
                            ttCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATc                                                       >  1:2288296/1‑93 (MQ=255)
                              cGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCaa                                                     <  1:6527450/93‑1 (MQ=255)
                               ggCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    <  2:5759173/93‑1 (MQ=255)
                               ggCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAt                                                    >  1:7278567/1‑93 (MQ=255)
                                gCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATa                                                   >  1:1444019/1‑93 (MQ=255)
                                 cATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATaa                                                  >  2:6369019/1‑93 (MQ=255)
                                  aTCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAg                                                 >  1:6072277/1‑93 (MQ=255)
                                  aTCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAg                                                 <  1:955494/93‑1 (MQ=255)
                                   tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                <  1:4720117/93‑1 (MQ=255)
                                   tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                <  2:5168788/93‑1 (MQ=255)
                                   tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  1:4008140/1‑93 (MQ=255)
                                   tCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGc                                                >  2:3532644/1‑93 (MQ=255)
                                    cTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCt                                               <  2:3963329/93‑1 (MQ=255)
                                     ttGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTc                                              >  2:2061656/1‑93 (MQ=255)
                                      tGCGGCGCACCGCGGCGTTCCCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCg                                             <  1:4800857/93‑1 (MQ=255)
                                          gcgcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGt                                         <  1:7495099/93‑1 (MQ=255)
                                          gcgcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGt                                         >  2:5031136/1‑93 (MQ=255)
                                          gcgcACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGt                                         >  1:6636111/1‑93 (MQ=255)
                                             cACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGc                                      >  1:5747845/1‑93 (MQ=255)
                                               ccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCtc                                    >  1:1033591/1‑92 (MQ=255)
                                               ccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCtc                                    >  1:5616733/1‑92 (MQ=255)
                                               ccGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTa                                    >  2:2926607/1‑93 (MQ=255)
                                                cgcgGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                   <  1:3753969/93‑1 (MQ=255)
                                                cgcgGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAc                                   <  1:1946812/93‑1 (MQ=255)
                                                 gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:3176234/1‑93 (MQ=255)
                                                 gcggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTAcc                                  >  2:4954925/1‑93 (MQ=255)
                                                   ggcgTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCaa                                <  1:4164810/93‑1 (MQ=255)
                                                      gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             >  1:3512171/1‑93 (MQ=255)
                                                      gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             >  1:5114963/1‑93 (MQ=255)
                                                      gTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTg                             <  1:6024706/93‑1 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:271398/1‑93 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:2342324/1‑93 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  1:3311329/1‑93 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:6406154/1‑93 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            <  1:5037030/93‑1 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  2:4135770/1‑93 (MQ=255)
                                                       ttCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTgg                            >  1:5419625/1‑93 (MQ=255)
                                                        tCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           <  2:1331254/93‑1 (MQ=255)
                                                        tCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGc                           >  1:6965843/1‑93 (MQ=255)
                                                         cACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCa                          >  1:4567646/1‑93 (MQ=255)
                                                          aCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAc                         <  2:7685067/93‑1 (MQ=255)
                                                          aCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAc                         >  2:4925899/1‑93 (MQ=255)
                                                           ccGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAcc                        >  1:249215/1‑93 (MQ=255)
                                                           ccGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAcc                        <  2:2689140/93‑1 (MQ=255)
                                                           ccGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCAcc                        <  1:7663114/93‑1 (MQ=255)
                                                             gcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                      >  1:2905577/1‑93 (MQ=255)
                                                             gcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                      >  2:4227965/1‑93 (MQ=255)
                                                             gcgAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTg                      <  2:3042469/93‑1 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  2:1791566/1‑93 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  2:1128163/1‑93 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  2:312467/1‑93 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    >  1:1310590/1‑93 (MQ=255)
                                                               gAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAc                    <  2:3575436/93‑1 (MQ=255)
                                                                 ataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAt                  <  1:2425820/93‑1 (MQ=255)
                                                                 ataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAt                  <  2:7293995/93‑1 (MQ=255)
                                                                 ataatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAt                  >  2:6949738/1‑93 (MQ=255)
                                                                  aaatCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATc                 <  2:6872482/92‑1 (MQ=255)
                                                                   aatCTGCGAACCGTCCGGCGCGCAGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATcc                <  2:4736395/93‑1 (MQ=255)
                                                                      cTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGcc             <  1:7376533/93‑1 (MQ=255)
                                                                         cGAACGGTCCGGCGCGCCGGCCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTc          <  1:3626245/93‑1 (MQ=255)
                                                                          gAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCg         >  1:689526/1‑93 (MQ=255)
                                                                          gAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCg         <  2:3635096/93‑1 (MQ=255)
                                                                           aaCCGTCCGGCGCGCCGTCCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCga        <  2:3251512/93‑1 (MQ=255)
                                                                            aCCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgag       >  1:2703530/1‑93 (MQ=255)
                                                                             ccGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgaga      >  2:7098447/1‑93 (MQ=255)
                                                                             ccGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCgaga      <  1:1187365/93‑1 (MQ=255)
                                                                              cGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAc     <  2:5544174/93‑1 (MQ=255)
                                                                               gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    >  2:1423656/1‑93 (MQ=255)
                                                                               gTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACg    <  2:3382809/93‑1 (MQ=255)
                                                                                      ||                                                                                      
GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG  >  NC_000913/3987500‑3987673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT  >  NC_000913/3987493‑3987674
                                                                                             |                                                                                        
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCCCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCG                                                                                          >  SRR4302543.5660599/1‑94 (MQ=60)
       GCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAC                                                                                   >  SRR4302543.4374992/1‑94 (MQ=60)
         TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                                                                 >  SRR4302543.2907218/1‑94 (MQ=60)
         TCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCA                                                                                 <  SRR4302543.6631724/94‑1 (MQ=60)
          CTTCTGCCAGCGAAATCTCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                <  SRR4302543.5203598/94‑1 (MQ=60)
          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                >  SRR4302543.4228031/1‑94 (MQ=60)
          CTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                >  SRR4302543.6482392/1‑94 (MQ=60)
          CTGCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAG                                                                                <  SRR4302543.1739452/94‑1 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                               <  SRR4302543.1242696/94‑1 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                               <  SRR4302543.4217532/94‑1 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                               <  SRR4302543.3285444/94‑1 (MQ=60)
           TTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGC                                                                               <  SRR4302543.1147104/94‑1 (MQ=60)
            TCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCG                                                                              <  SRR4302543.2183835/94‑1 (MQ=60)
               GCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCAC                                                                           <  SRR4302543.5909606/94‑1 (MQ=60)
               GCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCAC                                                                           <  SRR4302543.3762120/94‑1 (MQ=60)
                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                          >  SRR4302543.5130949/1‑94 (MQ=60)
                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                          >  SRR4302543.1935806/1‑94 (MQ=60)
                CCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACG                                                                          >  SRR4302543.955667/1‑94 (MQ=60)
                 CAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGC                                                                         >  SRR4302543.338412/1‑94 (MQ=60)
                  AGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCCCCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGCCCAGCGCACGCA                                                                        >  SRR4302543.7947512/1‑94 (MQ=60)
                  AGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCA                                                                        >  SRR4302543.962759/1‑94 (MQ=60)
                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                       >  SRR4302543.5110128/1‑94 (MQ=60)
                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                       <  SRR4302543.4015589/94‑1 (MQ=60)
                   GCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                       <  SRR4302543.3447565/94‑1 (MQ=60)
                   GCGAAATCCCCATTGTTTCGGCATCTGGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAG                                                                       <  SRR4302543.3447564/94‑1 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                      >  SRR4302543.3071595/1‑94 (MQ=60)
                    CGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGC                                                                      >  SRR4302543.7307132/1‑94 (MQ=60)
                     GAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                                                     >  SRR4302543.7406564/1‑94 (MQ=60)
                     GAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCC                                                                     >  SRR4302543.4416665/1‑94 (MQ=60)
                      AAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCA                                                                    <  SRR4302543.2115634/94‑1 (MQ=60)
                      AAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCCCCGACCAGCGCACGCAGCCA                                                                    <  SRR4302543.7268137/94‑1 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                  >  SRR4302543.3397259/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                  >  SRR4302543.2756130/1‑94 (MQ=60)
                        ATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                  >  SRR4302543.5858391/1‑94 (MQ=60)
                        ATCCCCATTTGATCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGA                                                                  >  SRR4302543.2235307/1‑94 (MQ=60)
                         TCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAT                                                                 <  SRR4302543.2618787/94‑1 (MQ=60)
                         TCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGAT                                                                 <  SRR4302543.7965918/94‑1 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                <  SRR4302543.390515/94‑1 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                >  SRR4302543.7061207/1‑94 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                >  SRR4302543.6779398/1‑94 (MQ=60)
                          CCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATT                                                                >  SRR4302543.1075903/1‑94 (MQ=60)
                           CCCATTTGTTCGGCGTCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                               >  SRR4302543.5275618/1‑94 (MQ=60)
                           CCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTT                                                               >  SRR4302543.4785891/1‑94 (MQ=60)
                            CCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTC                                                              <  SRR4302543.4571314/94‑1 (MQ=60)
                             CATTTGTTGGGCAGTTTGCGGCGCAGCGCGCCCTTCCCCGCGAATAATCTGCGAACCCTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                             <  SRR4302543.972497/94‑1 (MQ=42)
                             CATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                             <  SRR4302543.1419887/94‑1 (MQ=60)
                             CATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                             >  SRR4302543.167349/1‑94 (MQ=60)
                             CATGTGTTCGGCATCTTGCGGCGCAACGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGAGCAGCGCACGCAGCCAGATTTCG                                                             <  SRR4302543.1419886/94‑1 (MQ=60)
                             CAATTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCG                                                             <  SRR4302543.2703589/94‑1 (MQ=60)
                               TTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCC                                                           <  SRR4302543.3173311/94‑1 (MQ=60)
                                 TGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCAT                                                         >  SRR4302543.869228/1‑94 (MQ=60)
                                  GTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                                                        >  SRR4302543.7214069/1‑94 (MQ=60)
                                  GTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                                                        >  SRR4302543.2309190/1‑94 (MQ=60)
                                  GTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATC                                                        >  SRR4302543.1875033/1‑94 (MQ=60)
                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGCACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAA                                                     >  SRR4302543.3762363/1‑94 (MQ=60)
                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                     <  SRR4302543.123226/94‑1 (MQ=60)
                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                     <  SRR4302543.6588917/94‑1 (MQ=60)
                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                     >  SRR4302543.7348866/1‑94 (MQ=60)
                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                     <  SRR4302543.3887257/94‑1 (MQ=60)
                                     CGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAAT                                                     >  SRR4302543.2937914/1‑94 (MQ=60)
                                      GGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATA                                                    >  SRR4302543.1457063/1‑94 (MQ=60)
                                        CATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAG                                                  >  SRR4302543.6129827/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 <  SRR4302543.963869/94‑1 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.6847997/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.4043919/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.6847996/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.6531258/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.7725017/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.6277964/1‑94 (MQ=60)
                                         ATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGC                                                 >  SRR4302543.8002469/1‑94 (MQ=60)
                                          TCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCCGCGCACCCAGCCAGATTTAGCCATCAATAAGCT                                                >  SRR4302543.415280/1‑94 (MQ=60)
                                          TCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                <  SRR4302543.2477998/94‑1 (MQ=60)
                                          TCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                <  SRR4302543.4763247/94‑1 (MQ=60)
                                          TCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                <  SRR4302543.7489966/94‑1 (MQ=60)
                                          TCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCT                                                <  SRR4302543.1562240/94‑1 (MQ=60)
                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                              <  SRR4302543.968877/94‑1 (MQ=60)
                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                              <  SRR4302543.7208526/94‑1 (MQ=60)
                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                              <  SRR4302543.4859091/94‑1 (MQ=60)
                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                              <  SRR4302543.735577/94‑1 (MQ=60)
                                            TTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                              <  SRR4302543.4787677/94‑1 (MQ=60)
                                            CTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCG                                              <  SRR4302543.968876/94‑1 (MQ=60)
                                             TGCGGCGCACCGCGGCGTTCCCCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGG                                             <  SRR4302543.4844733/94‑1 (MQ=60)
                                             TGCGCCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGG                                             <  SRR4302543.250355/94‑1 (MQ=60)
                                               CGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCG                                           <  SRR4302543.2352424/94‑1 (MQ=60)
                                                GGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGT                                          >  SRR4302543.3046098/1‑94 (MQ=60)
                                                GGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGT                                          >  SRR4302543.6698732/1‑94 (MQ=60)
                                                 GCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTA                                         <  SRR4302543.7566796/94‑1 (MQ=60)
                                                 GCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTA                                         <  SRR4302543.1873357/94‑1 (MQ=60)
                                                  CGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAG                                        >  SRR4302543.5824617/1‑94 (MQ=60)
                                                   GCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGC                                       >  SRR4302543.5800235/1‑94 (MQ=60)
                                                   GCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGC                                       <  SRR4302543.2913785/94‑1 (MQ=60)
                                                   GCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGC                                       <  SRR4302543.3362596/94‑1 (MQ=60)
                                                    CACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCT                                      >  SRR4302543.3581605/1‑94 (MQ=60)
                                                     ACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTC                                     >  SRR4302543.1042769/1‑94 (MQ=60)
                                                     ACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTC                                     >  SRR4302543.5667916/1‑94 (MQ=60)
                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                   <  SRR4302543.1964288/94‑1 (MQ=60)
                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                   <  SRR4302543.211326/94‑1 (MQ=60)
                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                   <  SRR4302543.3786995/94‑1 (MQ=60)
                                                       CGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACC                                   >  SRR4302543.6108622/1‑94 (MQ=60)
                                                          GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                <  SRR4302543.909396/94‑1 (MQ=60)
                                                          GGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAAT                                <  SRR4302543.4202521/94‑1 (MQ=60)
                                                           GCGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATT                               >  SRR4302543.1941145/1‑94 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                              <  SRR4302543.5643650/94‑1 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                              <  SRR4302543.347021/94‑1 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                              >  SRR4302543.3543549/1‑94 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                              >  SRR4302543.5161235/1‑94 (MQ=60)
                                                            CGTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTG                              <  SRR4302543.1991566/94‑1 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                             <  SRR4302543.6080748/94‑1 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                             >  SRR4302543.4678346/1‑94 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                             >  SRR4302543.460744/1‑94 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                             >  SRR4302543.3581788/1‑94 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                             >  SRR4302543.3341215/1‑94 (MQ=60)
                                                             GTTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGG                             >  SRR4302543.5468908/1‑94 (MQ=60)
                                                              TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                            >  SRR4302543.7031395/1‑94 (MQ=60)
                                                              TTCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGC                            <  SRR4302543.5082844/94‑1 (MQ=60)
                                                               TCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCA                           <  SRR4302543.6457236/94‑1 (MQ=60)
                                                               TCACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCA                           >  SRR4302543.4609192/1‑94 (MQ=60)
                                                                 ACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACC                         >  SRR4302543.251381/1‑94 (MQ=60)
                                                                 ACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACC                         <  SRR4302543.5976021/94‑1 (MQ=60)
                                                                 ACCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACC                         >  SRR4302543.5792639/1‑94 (MQ=60)
                                                                  CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                        <  SRR4302543.7735747/94‑1 (MQ=60)
                                                                  CCGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCT                        <  SRR4302543.3744573/94‑1 (MQ=60)
                                                                   CGCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTG                       >  SRR4302543.2931994/1‑94 (MQ=60)
                                                                    GCGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGA                      >  SRR4302543.7593951/1‑94 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                     >  SRR4302543.6099824/1‑94 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                     <  SRR4302543.8010482/94‑1 (MQ=60)
                                                                     CGAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                     >  SRR4302543.1322238/1‑94 (MQ=60)
                                                                     CGAAGAATCTGCGAACCGTCCGGCGCTCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGAC                     <  SRR4302543.5641455/94‑1 (MQ=60)
                                                                      GAGTAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                    >  SRR4302543.4908720/1‑94 (MQ=60)
                                                                      GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                    <  SRR4302543.3977283/94‑1 (MQ=60)
                                                                      GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                    >  SRR4302543.1722068/1‑94 (MQ=60)
                                                                      GAATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACA                    <  SRR4302543.6486476/94‑1 (MQ=60)
                                                                       AATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAT                   >  SRR4302543.5731836/1‑94 (MQ=60)
                                                                       AATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAT                   <  SRR4302543.4078614/94‑1 (MQ=60)
                                                                       AATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACAT                   <  SRR4302543.5998262/94‑1 (MQ=60)
                                                                        ATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATC                  <  SRR4302543.1349244/94‑1 (MQ=60)
                                                                        ATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATC                  <  SRR4302543.2447714/94‑1 (MQ=60)
                                                                        ATAATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATC                  <  SRR4302543.291635/94‑1 (MQ=60)
                                                                          AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                >  SRR4302543.5825065/1‑94 (MQ=60)
                                                                          AATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCG                <  SRR4302543.4842510/94‑1 (MQ=60)
                                                                           ATCTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGC               >  SRR4302543.6681981/1‑94 (MQ=60)
                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT             >  SRR4302543.3790700/1‑94 (MQ=60)
                                                                             CTGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCT             <  SRR4302543.7447441/94‑1 (MQ=60)
                                                                              TGCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTT            >  SRR4302543.2509171/1‑94 (MQ=60)
                                                                               GCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTC           <  SRR4302543.2442063/94‑1 (MQ=60)
                                                                               GCGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTC           <  SRR4302543.7423022/94‑1 (MQ=60)
                                                                                CGAACGGTCCGGCGCGCCGGCCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCG          <  SRR4302543.3658183/94‑1 (MQ=60)
                                                                                CGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCG          >  SRR4302543.6966706/1‑94 (MQ=60)
                                                                                CGAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCG          >  SRR4302543.695862/1‑94 (MQ=60)
                                                                                 GAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA         <  SRR4302543.164753/94‑1 (MQ=60)
                                                                                 GAACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGA         <  SRR4302543.7862346/94‑1 (MQ=60)
                                                                                  AACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAG        <  SRR4302543.983549/94‑1 (MQ=60)
                                                                                  AACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAG        >  SRR4302543.2728059/1‑94 (MQ=60)
                                                                                   ACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGA       <  SRR4302543.5703217/94‑1 (MQ=60)
                                                                                   ACCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGA       >  SRR4302543.7488535/1‑94 (MQ=60)
                                                                                    CCGTCCGGCGCTCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC      <  SRR4302543.4611246/94‑1 (MQ=60)
                                                                                    CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC      <  SRR4302543.2731360/94‑1 (MQ=60)
                                                                                    CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC      <  SRR4302543.5123793/94‑1 (MQ=60)
                                                                                    CCGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGAC      <  SRR4302543.1198077/94‑1 (MQ=60)
                                                                                     CGTCCGGCGCGCCGATCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG     <  SRR4302543.6442747/94‑1 (MQ=60)
                                                                                     CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG     <  SRR4302543.3981411/94‑1 (MQ=60)
                                                                                     CGTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACG     <  SRR4302543.7027794/94‑1 (MQ=60)
                                                                                      GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG    <  SRR4302543.7942956/94‑1 (MQ=60)
                                                                                      GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG    >  SRR4302543.5055543/1‑94 (MQ=60)
                                                                                      GTCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGG    >  SRR4302543.4485299/1‑94 (MQ=60)
                                                                                       TCCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGG   <  SRR4302543.7067290/94‑1 (MQ=60)
                                                                                        CCGGCGCGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT  <  SRR4302543.3022317/94‑1 (MQ=60)
                                                                                             |                                                                                        
TTCAGTAGCTCTTCTGCCAGCGAAATCCCCATTTGTTCGGCATCTTGCGGCGCACCGCGGCGTTCACCGCGAATAATCTGCGAACCGTCCGGCCGGCCGACCAGCGCACGCAGCCAGATTTCGCCATCAATAAGCTCGGCGTAGCTACCAATTGGCACCTGACATCCGCCTTCGAGACGGGT  >  NC_000913/3987493‑3987674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑