Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,984 +CG coding (1516/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9831.G99.7% 982.1 / ‑4.3 319coding (1515/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (172/146);  total (172/147)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01
*NC_0009134,004,9832.C99.7% 976.2 / ‑4.3 318coding (1515/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (171/146);  total (171/147)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 338 total aligned reads displayed.

CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC  >  NC_000913/4004894‑4005068
                                                                                           ||                                                                                     
cGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gc                                                                                     <  2:3287214/93‑2 (MQ=255)
cGCCAGCTGATTCACCTCGGACTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAAATGCCGCCCGCCCGGTGCTGCGCg‑‑gc                                                                                     >  2:2215818/1‑92 (MQ=255)
  ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                                   <  2:3667768/93‑4 (MQ=255)
          ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCtt                                                                             >  1:7798525/1‑93 (MQ=255)
             aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGc                                                                          >  2:4177175/1‑93 (MQ=255)
             aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGc                                                                          <  2:660988/93‑1 (MQ=255)
             aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGc                                                                          >  2:5444291/1‑93 (MQ=255)
              ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCa                                                                         >  1:113974/1‑93 (MQ=255)
              ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCa                                                                         >  1:3497861/1‑93 (MQ=255)
              ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCa                                                                         <  1:301624/93‑1 (MQ=255)
               cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCaa                                                                        >  2:1150307/1‑93 (MQ=255)
               cTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCaa                                                                        >  1:7005120/1‑93 (MQ=255)
                tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAc                                                                       >  1:44670/1‑93 (MQ=255)
                 cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACt                                                                      <  1:5371620/93‑1 (MQ=255)
                  ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACtt                                                                     >  1:7650989/1‑93 (MQ=255)
                  ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACtt                                                                     <  2:7621874/93‑1 (MQ=255)
                  ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACtt                                                                     <  2:4812490/93‑1 (MQ=255)
                   gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtg                                                                    >  2:3082165/1‑93 (MQ=255)
                     cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                                  >  1:270016/1‑93 (MQ=255)
                     cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                                  >  2:3141302/1‑93 (MQ=255)
                     cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                                  >  1:5836835/1‑93 (MQ=255)
                     cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgcc                                                                  >  2:5277004/1‑93 (MQ=255)
                      tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                                 >  2:1894144/1‑93 (MQ=255)
                      tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccg                                                                 <  2:7844725/93‑1 (MQ=255)
                       ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                                <  2:2096048/93‑1 (MQ=255)
                       ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgt                                                                <  2:3685222/93‑1 (MQ=255)
                        gTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtg                                                               >  1:4632682/1‑93 (MQ=255)
                        gTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtg                                                               >  2:1991874/1‑93 (MQ=255)
                          gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                             >  2:4948487/1‑93 (MQ=255)
                          gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                             <  1:2843632/93‑1 (MQ=255)
                          gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTtgccgtgcc                                                             <  1:4605006/93‑1 (MQ=255)
                              cAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGt                                                         >  2:5364152/1‑93 (MQ=255)
                               aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                        <  2:3803172/93‑1 (MQ=255)
                                aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                                       <  1:2233554/93‑1 (MQ=255)
                                  atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                                     <  2:2838840/93‑1 (MQ=255)
                                   tatTGCCCAGCATTCTGCCCTTCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGTGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                                    >  2:7021484/1‑93 (MQ=255)
                                   tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                                    <  1:6611292/93‑1 (MQ=255)
                                      tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                                 >  1:6723880/1‑93 (MQ=255)
                                      tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                                 <  2:6222166/93‑1 (MQ=255)
                                      tGCCCAGCATTCTGCCCTACAACTGACAGAGGACGCGCGCCCGGTGCTGCGCGGCGAATGGTCTTTGCCACTTGCCGTGCCGCGTATCGTggg                                                 >  1:2006231/1‑92 (MQ=255)
                                         ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                              >  1:4217373/1‑93 (MQ=255)
                                         ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                              >  2:5884700/1‑93 (MQ=255)
                                           aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                            >  2:1438487/1‑93 (MQ=255)
                                             cATTCTGCACTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                          <  2:69373/93‑1 (MQ=255)
                                              aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                         <  1:7252572/93‑1 (MQ=255)
                                              aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                         <  2:2946259/93‑1 (MQ=255)
                                              aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                         >  2:2878277/1‑93 (MQ=255)
                                              aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                         <  2:2700532/93‑1 (MQ=255)
                                               ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                        >  1:2399026/1‑93 (MQ=255)
                                               ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                        >  1:6923975/1‑93 (MQ=255)
                                               ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                        >  1:6455063/1‑93 (MQ=255)
                                                 cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                                      >  2:7935055/1‑93 (MQ=255)
                                                  tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                                     <  2:3521832/93‑1 (MQ=255)
                                                   gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                                    <  1:161844/93‑1 (MQ=255)
                                                    cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                                   >  2:6966049/1‑93 (MQ=255)
                                                    cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGCGGCGCTCAAACCGAAAg                                   >  1:7044363/1‑93 (MQ=255)
                                                     ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                                  >  1:4803247/1‑93 (MQ=255)
                                                     ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                                  >  1:4896163/1‑93 (MQ=255)
                                                     ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                                  <  1:1475592/93‑1 (MQ=255)
                                                     ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                                  >  2:3399525/1‑93 (MQ=255)
                                                      cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                                 >  2:3701670/1‑93 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                <  1:3692613/93‑1 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                <  1:4271495/93‑1 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                >  1:1965622/1‑93 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                <  2:7444256/93‑1 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                >  2:7737765/1‑93 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                >  1:7907027/1‑93 (MQ=255)
                                                       tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                                >  2:2546446/1‑93 (MQ=255)
                                                        aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                               >  1:3587462/1‑93 (MQ=255)
                                                         cAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATg                              <  2:1708105/93‑1 (MQ=255)
                                                          aaCTGACAGAGGCGTCGCGCCGGGTGCTGCTCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                             <  2:4602661/93‑1 (MQ=255)
                                                          aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                             >  2:1300781/1‑93 (MQ=255)
                                                          aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                             >  2:2549697/1‑93 (MQ=255)
                                                           aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                            >  1:5623825/1‑93 (MQ=255)
                                                             tGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGa                          >  2:1597666/1‑93 (MQ=255)
                                                             cgACAGAGGCCGCTCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCCCTCAAACCGAAAGCGATGCAGa                          <  2:6305732/92‑1 (MQ=255)
                                                                cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt                       <  1:6077675/93‑1 (MQ=255)
                                                                cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt                       >  1:1356787/1‑93 (MQ=255)
                                                                cAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt                       <  2:5700512/93‑1 (MQ=255)
                                                                 agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc                      >  2:767360/1‑93 (MQ=255)
                                                                 agagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc                      <  1:1094663/93‑1 (MQ=255)
                                                                  gagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg                     <  1:7431288/93‑1 (MQ=255)
                                                                  gagGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg                     >  2:4286642/1‑93 (MQ=255)
                                                                   agGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt                    <  2:5100565/93‑1 (MQ=255)
                                                                     gCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCCACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt                   >  2:4409193/1‑92 (MQ=255)
                                                                     gCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc                  <  1:1734933/93‑1 (MQ=255)
                                                                      ccGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg                 <  1:4049277/93‑1 (MQ=255)
                                                                      ccGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg                 <  1:2860832/93‑1 (MQ=255)
                                                                        gcgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGGAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc               >  1:2556913/1‑93 (MQ=255)
                                                                        ccgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc               <  2:2324134/92‑1 (MQ=255)
                                                                         cgcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg              <  1:7887727/93‑1 (MQ=255)
                                                                          gcgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg             >  2:3920792/1‑93 (MQ=255)
                                                                           cgcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCggcggc            <  2:4256048/93‑1 (MQ=255)
                                                                            gcCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa           <  1:7126783/93‑1 (MQ=255)
                                                                             cccGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa          <  2:467909/93‑1 (MQ=255)
                                                                             cccGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa          >  1:5959683/1‑93 (MQ=255)
                                                                             cccGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa          >  2:7101112/1‑93 (MQ=255)
                                                                             cccGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa          >  1:4080480/1‑93 (MQ=255)
                                                                             cccGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa          >  2:1842638/1‑93 (MQ=255)
                                                                                  tGCG‑CGCC‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCACCTATGATc  <  2:3573529/93‑1 (MQ=255)
                                                                                           ||                                                                                     
CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC  >  NC_000913/4004894‑4005068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  NC_000913/4004892‑4005077
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TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑G                                                                                               <  SRR4302543.954988/94‑1 (MQ=60)
          GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCT                                                                                       <  SRR4302543.6944320/94‑1 (MQ=60)
           ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTT                                                                                      >  SRR4302543.954730/1‑94 (MQ=60)
           ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTT                                                                                      >  SRR4302543.7872014/1‑94 (MQ=60)
           ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTT                                                                                      <  SRR4302543.4647720/94‑1 (MQ=60)
            TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTT                                                                                     <  SRR4302543.4074519/94‑1 (MQ=60)
            TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTT                                                                                     <  SRR4302543.7231281/94‑1 (MQ=60)
             TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTG                                                                                    >  SRR4302543.1464622/1‑94 (MQ=60)
             TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTG                                                                                    <  SRR4302543.4704331/94‑1 (MQ=60)
              CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGC                                                                                   >  SRR4302543.361952/1‑94 (MQ=60)
              CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGC                                                                                   >  SRR4302543.6813757/1‑94 (MQ=60)
               ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCA                                                                                  >  SRR4302543.115012/1‑94 (MQ=60)
               ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCA                                                                                  >  SRR4302543.3529150/1‑94 (MQ=60)
                CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAA                                                                                 <  SRR4302543.304335/94‑1 (MQ=60)
                CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAA                                                                                 >  SRR4302543.7071684/1‑94 (MQ=60)
                 CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAAC                                                                                >  SRR4302543.45076/1‑94 (MQ=60)
                  TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                               <  SRR4302543.1308188/94‑1 (MQ=60)
                  TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                               <  SRR4302543.5780034/94‑1 (MQ=60)
                  TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACT                                                                               <  SRR4302543.5209655/94‑1 (MQ=60)
                   CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTT                                                                              <  SRR4302543.5420256/94‑1 (MQ=60)
                   CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTT                                                                              <  SRR4302543.4740872/94‑1 (MQ=60)
                   CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTT                                                                              >  SRR4302543.7723537/1‑94 (MQ=60)
                    GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTG                                                                             <  SRR4302543.2806890/94‑1 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                                           >  SRR4302543.5889924/1‑94 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                                           >  SRR4302543.2013237/1‑94 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                                           >  SRR4302543.272390/1‑94 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                                           >  SRR4302543.4308221/1‑94 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCC                                                                           <  SRR4302543.2586374/94‑1 (MQ=60)
                       CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCG                                                                          >  SRR4302543.7793282/1‑94 (MQ=60)
                       CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCG                                                                          >  SRR4302543.4437574/1‑94 (MQ=60)
                       CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCG                                                                          >  SRR4302543.7386991/1‑94 (MQ=60)
                       CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATACTCTTTGCAACTTGCCG                                                                          >  SRR4302543.4306218/1‑94 (MQ=60)
                         GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                                        >  SRR4302543.4674793/1‑94 (MQ=60)
                         GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTG                                                                        <  SRR4302543.5755859/94‑1 (MQ=60)
                          GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGC                                                                       <  SRR4302543.5343607/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGCCAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                                      <  SRR4302543.2879569/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                                      <  SRR4302543.904506/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                                      <  SRR4302543.4243059/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCC                                                                      >  SRR4302543.6352925/1‑94 (MQ=60)
                            GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                                     >  SRR4302543.1126316/1‑94 (MQ=60)
                            GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                                     <  SRR4302543.4646838/94‑1 (MQ=60)
                            GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                                     <  SRR4302543.4648417/94‑1 (MQ=60)
                            GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCG                                                                     <  SRR4302543.2869610/94‑1 (MQ=60)
                                CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                                 >  SRR4302543.7880046/1‑94 (MQ=60)
                                  AAATATTGCCCAGCATTCTGCCCTACAATTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                               >  SRR4302543.4568216/1‑94 (MQ=60)
                                  AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                               <  SRR4302543.2253755/94‑1 (MQ=60)
                                   AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                              <  SRR4302543.7081554/94‑1 (MQ=60)
                                   AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                              >  SRR4302543.605068/1‑94 (MQ=60)
                                   AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                              >  SRR4302543.5372879/1‑94 (MQ=60)
                                    ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                                             >  SRR4302543.6702144/1‑94 (MQ=60)
                                     TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                                            <  SRR4302543.6673604/94‑1 (MQ=60)
                                     TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                                            <  SRR4302543.2857149/94‑1 (MQ=60)
                                     TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                                            <  SRR4302543.717375/94‑1 (MQ=60)
                                     TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                                            <  SRR4302543.5546888/94‑1 (MQ=60)
                                       TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                                          >  SRR4302543.6787416/1‑94 (MQ=60)
                                       TTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                                          >  SRR4302543.2024263/1‑94 (MQ=60)
                                       TTGCCCAGCATTCTGCCCTACAACTGACAGAGGACGCGCGCCCGGTGCTGCGCGGCGAATGGTCTTTGCCACTTGCCGTGCCGCGTATCGTGGG                                                          >  SRR4302543.2024262/1‑94 (MQ=60)
                                        TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                                         <  SRR4302543.4868663/94‑1 (MQ=60)
                                          CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                       >  SRR4302543.4255608/1‑94 (MQ=60)
                                           CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                                      >  SRR4302543.3557854/1‑94 (MQ=60)
                                            CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                                     >  SRR4302543.1760704/1‑94 (MQ=60)
                                            CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                                     >  SRR4302543.2939666/1‑94 (MQ=60)
                                            CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                                     <  SRR4302543.1720151/94‑1 (MQ=60)
                                               CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                  >  SRR4302543.695530/1‑94 (MQ=60)
                                               CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                  >  SRR4302543.1297534/1‑94 (MQ=60)
                                               CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                  <  SRR4302543.6138468/94‑1 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 >  SRR4302543.6515887/1‑94 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 >  SRR4302543.2420723/1‑94 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 >  SRR4302543.583423/1‑94 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 <  SRR4302543.7322701/94‑1 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 >  SRR4302543.6989077/1‑94 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 >  SRR4302543.5944400/1‑94 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 <  SRR4302543.7443810/94‑1 (MQ=60)
                                                ATTCGGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 <  SRR4302543.2420706/94‑1 (MQ=60)
                                                    CGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                                             <  SRR4302543.1619993/94‑1 (MQ=60)
                                                     GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                                            <  SRR4302543.163239/94‑1 (MQ=60)
                                                     GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGCGGCGCTCAAACCGAAAG                                            >  SRR4302543.7112526/1‑94 (MQ=60)
                                                     CCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                                            <  SRR4302543.2085151/94‑1 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           <  SRR4302543.7082679/94‑1 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           >  SRR4302543.4847138/1‑94 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           >  SRR4302543.4940795/1‑94 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           <  SRR4302543.2976792/94‑1 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTGTGCAACTTGCCGGGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           <  SRR4302543.2976793/94‑1 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.4911361/1‑94 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.2678926/1‑94 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.4579579/1‑94 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          <  SRR4302543.6940848/94‑1 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.3566494/1‑94 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.7311883/1‑94 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.3677970/1‑94 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          <  SRR4302543.1488891/94‑1 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302543.7967412/1‑94 (MQ=60)
                                                        CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                         >  SRR4302543.704897/1‑94 (MQ=60)
                                                        CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                         >  SRR4302543.704898/1‑94 (MQ=60)
                                                        CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                         >  SRR4302543.2189832/1‑94 (MQ=60)
                                                        CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                         >  SRR4302543.1983248/1‑94 (MQ=60)
                                                        CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                         <  SRR4302543.3451852/94‑1 (MQ=60)
                                                        CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                                         >  SRR4302543.7981326/1‑94 (MQ=60)
                                                         TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        <  SRR4302543.6632188/94‑1 (MQ=60)
                                                         TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        <  SRR4302543.3725070/94‑1 (MQ=60)
                                                         TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        <  SRR4302543.4310312/94‑1 (MQ=60)
                                                         TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        >  SRR4302543.3619142/1‑94 (MQ=60)
                                                           CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                                      <  SRR4302543.113194/94‑1 (MQ=60)
                                                           CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                                      >  SRR4302543.2501015/1‑94 (MQ=60)
                                                           CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                                      <  SRR4302543.7503648/94‑1 (MQ=60)
                                                            AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                                     >  SRR4302543.5675072/1‑94 (MQ=60)
                                                            AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                                     >  SRR4302543.4169789/1‑94 (MQ=60)
                                                              CTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGA                                   >  SRR4302543.7194544/1‑94 (MQ=60)
                                                              CTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGA                                   <  SRR4302543.780585/94‑1 (MQ=60)
                                                              CTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGA                                   <  SRR4302543.1793007/94‑1 (MQ=60)
                                                               TGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAA                                  >  SRR4302543.6600111/1‑94 (MQ=60)
                                                                 ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                                <  SRR4302543.4088720/94‑1 (MQ=60)
                                                                 ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                                >  SRR4302543.1368926/1‑94 (MQ=60)
                                                                  CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                               <  SRR4302543.6135279/94‑1 (MQ=60)
                                                                  CAGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                               <  SRR4302543.6003117/94‑1 (MQ=60)
                                                                   AGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                              <  SRR4302543.6758932/94‑1 (MQ=60)
                                                                   AGAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                              <  SRR4302543.1104513/94‑1 (MQ=60)
                                                                    GAGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT                             <  SRR4302543.7502534/94‑1 (MQ=60)
                                                                     AGGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT                            >  SRR4302543.366537/1‑94 (MQ=60)
                                                                      GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC                           <  SRR4302543.4101352/94‑1 (MQ=60)
                                                                      GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC                           <  SRR4302543.2576220/94‑1 (MQ=60)
                                                                      GGCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCGTTC                           <  SRR4302543.4449160/94‑1 (MQ=60)
                                                                       GCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG                          >  SRR4302543.4091229/1‑94 (MQ=60)
                                                                       GCCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG                          <  SRR4302543.1750398/94‑1 (MQ=60)
                                                                        CCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG                         <  SRR4302543.2886921/94‑1 (MQ=60)
                                                                        CCGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG                         <  SRR4302543.4085510/94‑1 (MQ=60)
                                                                         CGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGGAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                        >  SRR4302543.2580289/1‑94 (MQ=60)
                                                                         CGCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                        >  SRR4302543.39128/1‑94 (MQ=60)
                                                                         CCCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                        <  SRR4302543.566290/94‑1 (MQ=60)
                                                                          GCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG                       <  SRR4302543.155571/94‑1 (MQ=60)
                                                                          GCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG                       <  SRR4302543.7971126/94‑1 (MQ=60)
                                                                          GCGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG                       >  SRR4302543.7482765/1‑94 (MQ=60)
                                                                          GCGCGCCCGCTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG                       <  SRR4302543.791290/94‑1 (MQ=60)
                                                                           CGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG                      >  SRR4302543.3494636/1‑94 (MQ=60)
                                                                           CGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG                      <  SRR4302543.7961926/94‑1 (MQ=60)
                                                                           CGCGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG                      <  SRR4302543.7961925/94‑1 (MQ=60)
                                                                             CGCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA                    >  SRR4302543.1683476/1‑94 (MQ=60)
                                                                              GCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA                   >  SRR4302543.6014238/1‑94 (MQ=60)
                                                                              GCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA                   <  SRR4302543.7196025/94‑1 (MQ=60)
                                                                              GCCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA                   >  SRR4302543.4117055/1‑94 (MQ=60)
                                                                              GCCCGGTGCTGCGCGGCGAATCCTCTGTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA                   <  SRR4302543.2285569/94‑1 (MQ=60)
                                                                               CCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC                  <  SRR4302543.2873646/94‑1 (MQ=60)
                                                                               CCCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC                  <  SRR4302543.4648600/94‑1 (MQ=60)
                                                                                ccggtggtgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                 <  SRR4302543.6618197/79‑1 (MQ=60)
                                                                                CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                 >  SRR4302543.4199426/1‑94 (MQ=60)
                                                                                CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                 >  SRR4302543.7439666/1‑94 (MQ=60)
                                                                                CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                 >  SRR4302543.3334434/1‑94 (MQ=60)
                                                                                CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                 >  SRR4302543.3334435/1‑94 (MQ=60)
                                                                                CCGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCGGTCGGCGGCAACT                 >  SRR4302543.3334431/1‑94 (MQ=60)
                                                                                 CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                >  SRR4302543.7197681/1‑94 (MQ=60)
                                                                                 CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                >  SRR4302543.3316252/1‑94 (MQ=60)
                                                                                 CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                >  SRR4302543.708728/1‑94 (MQ=60)
                                                                                 CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                >  SRR4302543.5835689/1‑94 (MQ=60)
                                                                                 CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                <  SRR4302543.4735704/94‑1 (MQ=60)
                                                                                 CGGTGCTGCGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGCTCGGAGGCAACTA                >  SRR4302543.3316254/1‑94 (MQ=60)
                                                                                  ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT               >  SRR4302543.3657144/14‑94 (MQ=60)
                                                                                  ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT               >  SRR4302543.3509152/14‑94 (MQ=60)
                                                                                  ggtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTAT               <  SRR4302543.478343/81‑1 (MQ=60)
                                                                                   gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCTAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG              <  SRR4302543.4910905/82‑1 (MQ=60)
                                                                                   gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG              <  SRR4302543.6403362/82‑1 (MQ=60)
                                                                                   gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG              <  SRR4302543.6128009/82‑1 (MQ=60)
                                                                                   gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG              <  SRR4302543.7811026/82‑1 (MQ=60)
                                                                                   gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG              <  SRR4302543.802268/82‑1 (MQ=60)
                                                                                   gtgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATG              <  SRR4302543.3588103/82‑1 (MQ=60)
                                                                                    tgctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGA             <  SRR4302543.284978/83‑1 (MQ=60)
                                                                                    ctgcgcggG‑‑GAATCCTCTGTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC           <  SRR4302543.2596855/86‑1 (MQ=60)
                                                                                     cctgcgcggcGAATCCTCTTCGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGAT            <  SRR4302543.7795095/84‑1 (MQ=60)
                                                                                      ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC           <  SRR4302543.4657674/85‑1 (MQ=60)
                                                                                      ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC           >  SRR4302543.6274047/10‑94 (MQ=60)
                                                                                      ctgcgcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC           <  SRR4302543.6225017/85‑1 (MQ=60)
                                                                                         CGCGGCGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA        >  SRR4302543.392673/1‑94 (MQ=60)
                                                                                          gcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAA       >  SRR4302543.3108743/6‑94 (MQ=60)
                                                                                          gcggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAA       >  SRR4302543.3108742/6‑94 (MQ=60)
                                                                                            ggcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAAC     >  SRR4302543.3588597/4‑94 (MQ=60)
                                                                                            C‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG   <  SRR4302543.7131173/94‑1 (MQ=60)
                                                                                            C‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG   >  SRR4302543.7298155/1‑94 (MQ=60)
                                                                                             gcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACT    <  SRR4302543.7083935/92‑1 (MQ=60)
                                                                                             gcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACT    <  SRR4302543.697292/92‑1 (MQ=60)
                                                                                             gcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACT    <  SRR4302543.91818/92‑1 (MQ=60)
                                                                                             gcGAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACT    <  SRR4302543.5892252/92‑1 (MQ=60)
                                                                                               GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  SRR4302543.6278382/1‑94 (MQ=60)
                                                                                               GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  SRR4302543.1785680/1‑94 (MQ=60)
                                                                                               |                                                                                             
TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑GAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  NC_000913/4004892‑4005077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑