Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,491 2 bp→CG intergenic (+58/‑174) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4910GC99.7% 908.7 / ‑5.1 289intergenic (+58/‑175)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (144/144);  total (144/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
*NC_0009134,008,4920CG99.7% 897.0 / ‑5.5 285intergenic (+59/‑174)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (143/141);  total (143/142)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 298 total aligned reads displayed.

GCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008410‑4008577
                                                                                        ||                                                                                      
catGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCgcacca                                                                                   <  1:4499914/91‑1 (MQ=255)
  tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:7740586/1‑93 (MQ=255)
  tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:7608789/1‑93 (MQ=255)
  tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:2421290/1‑93 (MQ=255)
  tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:3600033/1‑93 (MQ=255)
  tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                                                 <  1:2771415/93‑1 (MQ=255)
  tGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGGGCAAG‑GCTGCATTATGGGCAGTGCGCACCAGc                                                                                 >  2:4367903/1‑93 (MQ=255)
     aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              >  1:1125954/1‑93 (MQ=255)
     aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              >  1:3747692/1‑93 (MQ=255)
     aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              <  2:5912240/93‑1 (MQ=255)
     aGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                                              <  1:3317964/93‑1 (MQ=255)
      ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCt                                                                             >  2:7423877/1‑93 (MQ=255)
      ggATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGAACCAGCGTCt                                                                             >  1:2486928/1‑93 (MQ=255)
                    ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                                               <  2:2031600/90‑1 (MQ=255)
                     tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                                              >  2:6181357/3‑93 (MQ=255)
                       gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCACCGTCTGAAAGACCTTCATCCCg                                                            >  2:4687845/1‑93 (MQ=255)
                         gACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                                          <  1:6093420/93‑1 (MQ=255)
                           cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                        >  2:4069166/1‑93 (MQ=255)
                           cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                        <  2:159736/93‑1 (MQ=255)
                             ggATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                     >  1:5049320/1‑92 (MQ=255)
                              gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    >  1:1429259/1‑91 (MQ=255)
                              gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                                    >  1:5866394/1‑91 (MQ=255)
                                tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  <  2:6190706/93‑5 (MQ=255)
                                tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                                                  >  2:6761975/1‑89 (MQ=255)
                                  aaCGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 <  2:3816410/93‑1 (MQ=255)
                                  aaCGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 >  1:10696/1‑93 (MQ=255)
                                  aaCGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                 <  1:554284/93‑1 (MQ=255)
                                   acgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                >  2:7913716/1‑93 (MQ=255)
                                   acgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                                                >  2:6333657/1‑93 (MQ=255)
                                    cgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                                               >  1:1715506/1‑93 (MQ=255)
                                     gacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              <  1:2305281/93‑1 (MQ=255)
                                     gacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              >  1:6261266/1‑93 (MQ=255)
                                     gacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                                              <  1:7913688/93‑1 (MQ=255)
                                      acgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             >  1:536081/1‑93 (MQ=255)
                                      acgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                             <  1:5254770/93‑1 (MQ=255)
                                        gCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                                           >  1:5693879/1‑93 (MQ=255)
                                         ccGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          <  1:5430293/93‑1 (MQ=255)
                                         ccGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          <  2:3334042/93‑1 (MQ=255)
                                         ccGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                          <  1:531320/93‑1 (MQ=255)
                                          cGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                         <  2:5223614/93‑1 (MQ=255)
                                           gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  2:2573989/1‑93 (MQ=255)
                                           gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  1:4371889/1‑93 (MQ=255)
                                           gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                        >  2:1792981/1‑93 (MQ=255)
                                            aaaTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                       >  2:4518351/1‑93 (MQ=255)
                                             aaTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                      >  1:2926225/1‑93 (MQ=255)
                                              aTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     <  2:2092876/93‑1 (MQ=255)
                                              aTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                     >  1:3959578/1‑93 (MQ=255)
                                               tGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                    >  2:7487104/1‑93 (MQ=255)
                                                gCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                   >  1:706945/1‑93 (MQ=255)
                                                gCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                   <  2:5753793/93‑1 (MQ=255)
                                                 gtGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCTCCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  <  1:2449116/92‑1 (MQ=255)
                                                 cTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                  <  1:2079924/93‑1 (MQ=255)
                                                  tGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAGGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 >  1:6826650/1‑93 (MQ=255)
                                                  tGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                 <  2:1402236/93‑1 (MQ=255)
                                                   gTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  1:7611442/93‑1 (MQ=255)
                                                   gTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga                                <  2:5250862/93‑1 (MQ=255)
                                                    tCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               >  2:5459699/1‑93 (MQ=255)
                                                    tCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               >  2:4268867/1‑93 (MQ=255)
                                                    tCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgac                               >  1:7489183/1‑93 (MQ=255)
                                                      gATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  1:3121312/93‑1 (MQ=255)
                                                      gATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             <  1:3494506/93‑1 (MQ=255)
                                                      gATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga                             >  1:904654/1‑93 (MQ=255)
                                                         ggcgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          <  1:7315174/89‑1 (MQ=255)
                                                         ggcgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                          <  2:1249801/89‑1 (MQ=255)
                                                           cgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        <  1:1247214/91‑1 (MQ=255)
                                                           cgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                        <  1:2114780/91‑1 (MQ=255)
                                                            gggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                       <  1:6716493/92‑1 (MQ=255)
                                                              gg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     <  1:1855578/93‑1 (MQ=255)
                                                              gg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     >  1:6452140/1‑93 (MQ=255)
                                                              gg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     <  2:6505524/93‑1 (MQ=255)
                                                              gg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                     <  2:1501405/93‑1 (MQ=255)
                                                                   aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                  >  1:7754870/4‑93 (MQ=255)
                                                                    aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                 <  1:7825113/91‑1 (MQ=255)
                                                                     ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                <  2:438906/92‑1 (MQ=255)
                                                                      gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt               >  1:4838368/1‑93 (MQ=255)
                                                                      gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCAGCAtt               >  2:2159751/1‑93 (MQ=255)
                                                                      gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCAtt               >  1:3251616/1‑93 (MQ=255)
                                                                       cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa              <  2:3010630/93‑1 (MQ=255)
                                                                       cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa              <  2:5713371/93‑1 (MQ=255)
                                                                        tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             >  2:4198469/1‑93 (MQ=255)
                                                                        tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             >  1:7562367/1‑93 (MQ=255)
                                                                        tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt             >  1:2628251/1‑93 (MQ=255)
                                                                         gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc            <  1:4800350/93‑1 (MQ=255)
                                                                          cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           >  1:7665601/1‑93 (MQ=255)
                                                                          cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt           <  2:6935099/93‑1 (MQ=255)
                                                                           aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg          >  2:6552520/1‑93 (MQ=255)
                                                                            ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  1:6508924/93‑1 (MQ=255)
                                                                            ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  1:3594124/93‑1 (MQ=255)
                                                                            ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  2:6421732/93‑1 (MQ=255)
                                                                            ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc         <  2:7237980/93‑1 (MQ=255)
                                                                            ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCAGACGACGCGGTGCCGCATTATCTGc         <  1:6936037/93‑1 (MQ=255)
                                                                              aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt       <  1:6168525/93‑1 (MQ=255)
                                                                              aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATAGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt       >  1:4094934/1‑93 (MQ=255)
                                                                               tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa      >  2:5047312/1‑93 (MQ=255)
                                                                                gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     >  2:3989070/1‑93 (MQ=255)
                                                                                gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     >  1:5144968/1‑93 (MQ=255)
                                                                                gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     >  2:3464045/1‑93 (MQ=255)
                                                                                gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     >  1:5860415/1‑93 (MQ=255)
                                                                                gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa     <  2:2203663/93‑1 (MQ=255)
                                                                                   cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct  >  2:4789749/1‑93 (MQ=255)
                                                                                        ||                                                                                      
GCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT  >  NC_000913/4008410‑4008577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 205 total aligned reads displayed.
CAGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GG‑‑AAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008408‑4008579
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agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACC                                                                                      >  SRR4302543.2702915/4‑94 (MQ=60)
agcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACC                                                                                      <  SRR4302543.7234219/91‑1 (MQ=60)
 gcCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCA                                                                                     <  SRR4302543.5632844/92‑1 (MQ=60)
  caTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                                    <  SRR4302543.4540817/92‑1 (MQ=58)
  CCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                                    >  SRR4302543.3851997/1‑94 (MQ=60)
  CCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                                    >  SRR4302543.4084655/1‑94 (MQ=60)
  CCTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                                    >  SRR4302543.6463479/1‑94 (MQ=60)
    TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                                  >  SRR4302543.1979670/1‑94 (MQ=57)
    TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                                  >  SRR4302543.1839287/1‑94 (MQ=57)
    TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                                  <  SRR4302543.2796645/94‑1 (MQ=57)
    TGAAGGAGTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                                  <  SRR4302543.5365798/94‑1 (MQ=50)
     GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGT                                                                                 >  SRR4302543.2765261/1‑94 (MQ=57)
      AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                                >  SRR4302543.1136172/1‑94 (MQ=57)
      AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                                >  SRR4302543.3780664/1‑94 (MQ=57)
      AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                                >  SRR4302543.3780665/1‑94 (MQ=57)
      AAGGAGTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                                >  SRR4302543.7909677/1‑94 (MQ=55)
       AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCT                                                                               >  SRR4302543.3579796/1‑94 (MQ=57)
       AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCT                                                                               <  SRR4302543.5163192/94‑1 (MQ=57)
       AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCT                                                                               <  SRR4302543.3347908/94‑1 (MQ=57)
       AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGAACCAGCGTCT                                                                               >  SRR4302543.2509457/1‑94 (MQ=50)
        GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTG                                                                              <  SRR4302543.6872428/94‑1 (MQ=60)
        GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTG                                                                              <  SRR4302543.533840/94‑1 (MQ=60)
         gattgtattgcggttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTACGGGCAGTG‑CGCACCAGCGTCTGA                                                                             <  SRR4302543.1414794/78‑1 (MQ=60)
         GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑TGCACCAGCGTCTGA                                                                             >  SRR4302543.295393/1‑94 (MQ=60)
         GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                                             >  SRR4302543.2061076/1‑94 (MQ=60)
         GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                                             >  SRR4302543.981170/1‑94 (MQ=60)
         GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                                             <  SRR4302543.7754916/94‑1 (MQ=60)
         GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                                             >  SRR4302543.5740662/1‑94 (MQ=60)
          ATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGCGCAGTC‑CGCACCACCGTCTGAA                                                                            >  SRR4302543.1426254/1‑94 (MQ=38)
            tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAG                                                                          >  SRR4302543.2322846/14‑94 (MQ=60)
             gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGA                                                                         >  SRR4302543.6201352/13‑94 (MQ=60)
             gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGA                                                                         >  SRR4302543.6201353/13‑94 (MQ=60)
             T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGGAAGAC                                                                        >  SRR4302543.5564796/1‑94 (MQ=60)
             T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGAC                                                                        <  SRR4302543.4513099/94‑1 (MQ=60)
             T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGAC                                                                        <  SRR4302543.4744975/94‑1 (MQ=60)
                TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCT                                                                      >  SRR4302543.4862517/1‑94 (MQ=60)
                 TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTT                                                                     <  SRR4302543.690787/94‑1 (MQ=60)
                 TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTT                                                                     <  SRR4302543.7824561/94‑1 (MQ=60)
                 TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTT                                                                     <  SRR4302543.4906814/94‑1 (MQ=60)
                  GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTC                                                                    <  SRR4302543.5948503/94‑1 (MQ=60)
                  GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTC                                                                    <  SRR4302543.4349461/94‑1 (MQ=60)
                  GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTC                                                                    <  SRR4302543.13925/94‑1 (MQ=60)
                   cgtttgggGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                                   <  SRR4302543.2721845/86‑1 (MQ=60)
                   CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302543.3357637/1‑94 (MQ=60)
                   CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302543.2396930/1‑94 (MQ=60)
                   CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                                   >  SRR4302543.2366387/1‑94 (MQ=60)
                    gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302543.5142485/89‑1 (MQ=60)
                    gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302543.4838417/89‑1 (MQ=60)
                    gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302543.427662/89‑1 (MQ=60)
                    gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302543.427661/89‑1 (MQ=60)
                    gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  >  SRR4302543.5440228/6‑94 (MQ=60)
                    gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  >  SRR4302543.3553128/6‑94 (MQ=60)
                    gttgggtggaGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                                  <  SRR4302543.5248183/84‑1 (MQ=60)
                     TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCCCCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302543.295656/94‑1 (MQ=60)
                     TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302543.7750920/94‑1 (MQ=60)
                     TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCC                                                               <  SRR4302543.5320369/94‑1 (MQ=60)
                      G‑‑GTGACTGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCG                                                              <  SRR4302543.5976552/94‑1 (MQ=60)
                      G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCG                                                              <  SRR4302543.872164/94‑1 (MQ=60)
                      G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCG                                                              <  SRR4302543.248878/94‑1 (MQ=60)
                         GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGA                                                             >  SRR4302543.4856664/1‑94 (MQ=60)
                          TGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                                            >  SRR4302543.7402725/1‑94 (MQ=60)
                           GACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGT                                                           >  SRR4302543.1827439/1‑94 (MQ=60)
                           GACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                                           <  SRR4302543.6151166/94‑1 (MQ=60)
                            ACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT                                                          <  SRR4302543.4992703/94‑1 (MQ=60)
                            ACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT                                                          <  SRR4302543.785671/94‑1 (MQ=60)
                             CGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        >  SRR4302543.7298166/1‑94 (MQ=60)
                             CGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        <  SRR4302543.2136092/94‑1 (MQ=60)
                             CGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        <  SRR4302543.7302850/94‑1 (MQ=60)
                             CGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                        >  SRR4302543.5929627/1‑94 (MQ=60)
                              GGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCTAGCT‑GG                                                       >  SRR4302543.4845302/1‑94 (MQ=60)
                              GGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                       >  SRR4302543.5095229/1‑94 (MQ=60)
                              GGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                       >  SRR4302543.6240515/1‑94 (MQ=60)
                              GGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                       >  SRR4302543.7606789/1‑94 (MQ=60)
                               GGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302543.1442188/1‑94 (MQ=60)
                               GGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302543.624410/1‑94 (MQ=60)
                               GGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302543.5919794/1‑94 (MQ=60)
                               GGATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                      >  SRR4302543.6546049/1‑94 (MQ=60)
                                GATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                     <  SRR4302543.7732543/94‑4 (MQ=60)
                                 ATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302543.7075981/1‑94 (MQ=60)
                                 ATGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                     >  SRR4302543.4824059/1‑94 (MQ=60)
                                  TGAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                    >  SRR4302543.1321156/1‑94 (MQ=60)
                                   GAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                   <  SRR4302543.2909976/94‑1 (MQ=60)
                                   GAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                   >  SRR4302543.7730964/1‑94 (MQ=60)
                                   GAACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                   >  SRR4302543.10788/1‑94 (MQ=60)
                                    AACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                  <  SRR4302543.559375/94‑1 (MQ=60)
                                     ACGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                 >  SRR4302543.1730837/1‑94 (MQ=60)
                                      CGACGCCGAAATGCTGTCGATGGCGTGG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                <  SRR4302543.4706919/94‑1 (MQ=60)
                                      CGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                >  SRR4302543.2649934/1‑94 (MQ=60)
                                      CGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                >  SRR4302543.6320317/1‑94 (MQ=60)
                                      CGACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                <  SRR4302543.2370731/94‑1 (MQ=60)
                                       GACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               >  SRR4302543.541049/1‑94 (MQ=60)
                                       GACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302543.5203767/94‑1 (MQ=60)
                                       GACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302543.7988034/94‑1 (MQ=60)
                                       GACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                               <  SRR4302543.2326281/94‑1 (MQ=60)
                                        ACGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                              <  SRR4302543.5302161/94‑1 (MQ=60)
                                         CGCCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                             >  SRR4302543.5745832/1‑94 (MQ=60)
                                           CCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                           <  SRR4302543.6204208/94‑1 (MQ=60)
                                           CCGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                           <  SRR4302543.536243/94‑1 (MQ=60)
                                            CGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302543.4411483/1‑94 (MQ=60)
                                            CGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302543.3122017/1‑94 (MQ=60)
                                            CGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          >  SRR4302543.6465545/1‑94 (MQ=60)
                                            CGAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                          <  SRR4302543.4964196/94‑1 (MQ=60)
                                             GAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         >  SRR4302543.7937687/1‑94 (MQ=60)
                                             GAAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                         <  SRR4302543.1272658/94‑1 (MQ=60)
                                              AAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                        >  SRR4302543.5777097/1‑94 (MQ=60)
                                              AAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                        >  SRR4302543.2952808/1‑94 (MQ=60)
                                              AAATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGAGTCTGAAAGACCTTCACCCCGAGCgggaagtgcttggacataa                                        >  SRR4302543.5777098/1‑75 (MQ=41)
                                               AATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       >  SRR4302543.3994637/1‑94 (MQ=60)
                                               AATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       <  SRR4302543.152812/94‑1 (MQ=60)
                                               AATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       <  SRR4302543.7101858/94‑1 (MQ=60)
                                               AATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                       <  SRR4302543.1146385/94‑1 (MQ=60)
                                                ATGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                      <  SRR4302543.5773731/94‑1 (MQ=60)
                                                 TGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     >  SRR4302543.5191478/1‑94 (MQ=60)
                                                 TGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     <  SRR4302543.1322505/94‑1 (MQ=60)
                                                 TGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     >  SRR4302543.2122130/1‑94 (MQ=60)
                                                 TGCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                     >  SRR4302543.713379/1‑94 (MQ=60)
                                                  GCTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                    >  SRR4302543.3157507/1‑94 (MQ=60)
                                                   GTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCTCCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   <  SRR4302543.2471202/94‑1 (MQ=55)
                                                   CTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302543.7638120/1‑94 (MQ=60)
                                                   CTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   >  SRR4302543.5742968/1‑94 (MQ=60)
                                                   CTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   <  SRR4302543.4489452/94‑1 (MQ=60)
                                                   CTGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                   <  SRR4302543.2098495/94‑1 (MQ=60)
                                                    TGTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                  >  SRR4302543.397923/1‑94 (MQ=60)
                                                     GTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302543.7683712/94‑1 (MQ=60)
                                                     GTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 <  SRR4302543.7672238/94‑1 (MQ=60)
                                                     GTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 >  SRR4302543.7560842/1‑94 (MQ=60)
                                                     GTCGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                 >  SRR4302543.6728615/1‑94 (MQ=60)
                                                       CGATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                               >  SRR4302543.912620/1‑94 (MQ=60)
                                                        GATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302543.2131549/94‑1 (MQ=60)
                                                        GATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302543.3149384/94‑1 (MQ=60)
                                                        GATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302543.539031/94‑1 (MQ=60)
                                                        GATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                              <  SRR4302543.3525775/94‑1 (MQ=60)
                                                         ATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                             <  SRR4302543.686099/94‑1 (MQ=60)
                                                         ATGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                             >  SRR4302543.3976406/1‑94 (MQ=60)
                                                          TGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                            <  SRR4302543.5734891/94‑1 (MQ=60)
                                                          TGGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                            <  SRR4302543.1278147/94‑1 (MQ=60)
                                                           GGCG‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCCA‑CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302543.6176628/94‑1 (MQ=60)
                                                           GGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302543.969787/94‑1 (MQ=60)
                                                           GGCG‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                           <  SRR4302543.7385665/94‑1 (MQ=60)
                                                              G‑GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                       >  SRR4302543.6512940/1‑94 (MQ=60)
                                                               cggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         <  SRR4302543.2133712/85‑1 (MQ=60)
                                                               cggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                         <  SRR4302543.1258409/85‑1 (MQ=60)
                                                                gggtaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302543.7244095/86‑1 (MQ=60)
                                                                ggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302543.6779977/86‑1 (MQ=60)
                                                                ggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                        <  SRR4302543.5682819/86‑1 (MQ=60)
                                                                GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                      >  SRR4302543.2307215/1‑94 (MQ=60)
                                                                GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                      <  SRR4302543.1872261/94‑1 (MQ=60)
                                                                GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCC                      >  SRR4302543.4127525/1‑94 (MQ=60)
                                                                    AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                    >  SRR4302543.7828079/1‑94 (MQ=60)
                                                                     AG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                  >  SRR4302543.6903147/1‑94 (MQ=60)
                                                                     AG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                  <  SRR4302543.7898816/94‑1 (MQ=60)
                                                                      G‑GCTGCATTATGGGCAGTG‑CGCACCCGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACGACGCGGTGCCGCATT                 >  SRR4302543.6969584/1‑94 (MQ=60)
                                                                      G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                 <  SRR4302543.885511/94‑1 (MQ=60)
                                                                      G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                 >  SRR4302543.3281012/1‑94 (MQ=60)
                                                                      G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                 >  SRR4302543.4882494/1‑94 (MQ=60)
                                                                      G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCATT                 >  SRR4302543.3281011/1‑94 (MQ=60)
                                                                        GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                <  SRR4302543.6517378/94‑1 (MQ=60)
                                                                        GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                <  SRR4302543.2409399/94‑1 (MQ=60)
                                                                         CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               >  SRR4302543.7815043/1‑94 (MQ=60)
                                                                         CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               >  SRR4302543.2652034/1‑94 (MQ=60)
                                                                         CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               <  SRR4302543.2806068/94‑1 (MQ=60)
                                                                         CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               >  SRR4302543.7634347/1‑94 (MQ=60)
                                                                         CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               >  SRR4302543.201476/1‑94 (MQ=60)
                                                                         CTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT               >  SRR4302543.3510758/1‑94 (MQ=60)
                                                                          TGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              >  SRR4302543.5424882/1‑94 (MQ=60)
                                                                          TGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC              >  SRR4302543.6637623/1‑94 (MQ=60)
                                                                           GCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             <  SRR4302543.4844220/94‑1 (MQ=60)
                                                                           GCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             >  SRR4302543.7738255/1‑94 (MQ=60)
                                                                           GCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT             >  SRR4302543.4432785/1‑94 (MQ=60)
                                                                            CATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302543.2498631/94‑1 (MQ=60)
                                                                            CATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG            <  SRR4302543.7606350/94‑1 (MQ=60)
                                                                             ATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302543.1094057/1‑94 (MQ=60)
                                                                             ATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           <  SRR4302543.1227199/94‑1 (MQ=60)
                                                                             ATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302543.4328323/1‑94 (MQ=60)
                                                                             ATTATGGGCAGTG‑CGCACAAGAGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC           >  SRR4302543.3242304/1‑94 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          >  SRR4302543.7390700/1‑94 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302543.6570235/94‑1 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302543.341883/94‑1 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302543.7922343/94‑1 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          >  SRR4302543.5547157/1‑94 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302543.5527107/94‑1 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302543.3625850/94‑1 (MQ=60)
                                                                              TTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCAGACGACGCGGTGCCGCATTATCTGCG          <  SRR4302543.7001276/94‑1 (MQ=60)
                                                                               TATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         >  SRR4302543.4131686/1‑94 (MQ=60)
                                                                               TATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         <  SRR4302543.3654358/94‑1 (MQ=60)
                                                                               TATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATAGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT         >  SRR4302543.4131685/1‑94 (MQ=60)
                                                                                ATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        <  SRR4302543.6226886/94‑1 (MQ=60)
                                                                                ATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        >  SRR4302543.2187682/1‑94 (MQ=60)
                                                                                ATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA        >  SRR4302543.5917811/1‑94 (MQ=60)
                                                                                 TGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA       >  SRR4302543.5191479/1‑94 (MQ=60)
                                                                                 TGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA       >  SRR4302543.5913752/1‑94 (MQ=60)
                                                                                 TGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA       >  SRR4302543.5913754/1‑94 (MQ=60)
                                                                                  GGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA      <  SRR4302543.152261/94‑1 (MQ=60)
                                                                                   GGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC     >  SRR4302543.4110678/1‑94 (MQ=60)
                                                                                   GGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC     <  SRR4302543.2857216/94‑1 (MQ=60)
                                                                                    GCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT    <  SRR4302543.3317571/94‑1 (MQ=60)
                                                                                     CAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   <  SRR4302543.5271848/94‑1 (MQ=60)
                                                                                     CAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC   >  SRR4302543.491323/1‑94 (MQ=60)
                                                                                      AGTG‑CGCACCAGCGTCTGAAATACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACTACGCGGTGCCGCATTATCTGCGTAAACTCT  >  SRR4302543.3847547/1‑94 (MQ=60)
                                                                                      AGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  SRR4302543.4642525/1‑94 (MQ=60)
                                                                                           |                                                                                         
CAGCTGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCG‑GG‑‑AAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT  >  NC_000913/4008408‑4008579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑