Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,043,237 A→G intergenic (+28/+13) b3861 → / ← yihG putative GTP‑binding protein/putative endonuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,043,2370AG99.8% 1330.1 / ‑5.4 420intergenic (+28/+13)b3861/yihGputative GTP‑binding protein/putative endonuclease
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (209/209);  total (211/209)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 438 total aligned reads displayed.

TTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATT  >  NC_000913/4043147‑4043326
                                                                                          |                                                                                         
 ttAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGc                                                                                        <  1:1178393/93‑1 (MQ=255)
 ttAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGc                                                                                        <  2:5891646/93‑1 (MQ=255)
 ttAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGc                                                                                        <  2:4812384/93‑1 (MQ=255)
 ttAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGc                                                                                        <  1:4958808/93‑1 (MQ=255)
  tAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCg                                                                                       <  2:3770337/93‑1 (MQ=255)
  tAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCg                                                                                       <  1:7183904/93‑1 (MQ=255)
  tAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCg                                                                                       <  1:4342465/93‑1 (MQ=255)
      ggACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTa                                                                                   <  2:846281/93‑1 (MQ=255)
       gACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGGGCGTaa                                                                                  >  2:3126461/1‑93 (MQ=255)
       gACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTaa                                                                                  <  2:2967290/93‑1 (MQ=255)
        aCAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTaaa                                                                                 <  1:4629695/93‑1 (MQ=255)
          aGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTTATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAac                                                                               >  2:6892900/1‑93 (MQ=255)
            gagaTGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAactt                                                                             >  1:874371/1‑93 (MQ=255)
             agaTGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAactta                                                                            <  2:5395861/93‑1 (MQ=255)
               aTGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAacttact                                                                          <  1:1864506/93‑1 (MQ=255)
                tGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAacttactt                                                                         <  2:3705749/93‑1 (MQ=255)
                 gAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAActtactta                                                                        <  1:7505500/93‑1 (MQ=255)
                    cGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTc                                                                     <  1:3226714/93‑1 (MQ=255)
                    cGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTc                                                                     <  2:3336297/93‑1 (MQ=255)
                     gaagaagaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCt                                                                    >  1:6239429/1‑93 (MQ=255)
                      aagaagaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTg                                                                   >  2:7778573/1‑93 (MQ=255)
                      aagaagaaTTTATGTCTCGTGCCGGACGTTTTGGTCATTAAAATACTAACTCGCCTCTGCTCATTCAGGGGCGAGGAAAACTTACTTATTCTg                                                                   >  1:4894512/1‑93 (MQ=255)
                       agaagaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGa                                                                  <  2:4592808/93‑1 (MQ=255)
                       agaagaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGCGGCGAGTAAAACTTACTTATTCTgc                                                                  >  1:2162040/1‑92 (MQ=255)
                       agaagaaTTTATGTCTCGTGCCGGACGTTTTGGTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGa                                                                  >  2:2356337/1‑93 (MQ=255)
                        gaagaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGAc                                                                 <  2:3566572/93‑1 (MQ=255)
                        gaagaaTTTATGTCTCGTGCCAGACGTTTTGTTCATTAAAATACTCACCCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGAc                                                                 <  1:3714487/93‑1 (MQ=255)
                           gaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCt                                                              >  2:397784/1‑93 (MQ=255)
                           gaaTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCt                                                              <  1:5431588/93‑1 (MQ=255)
                             aTTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCtgt                                                            >  2:1640792/1‑93 (MQ=255)
                               ttATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGtga                                                          <  1:4261135/93‑1 (MQ=255)
                               ttATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGtga                                                          <  2:5819075/93‑1 (MQ=255)
                               ttATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGtga                                                          <  1:1373261/93‑1 (MQ=255)
                               ttATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGtga                                                          <  1:1536008/93‑1 (MQ=255)
                                tATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAAATTACTTATTCTGACGCTGtgat                                                         >  1:5653223/1‑93 (MQ=255)
                                 aTGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGtgatg                                                        >  1:2836900/1‑93 (MQ=255)
                                 aTGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGtgatg                                                        >  1:5997662/1‑93 (MQ=255)
                                   gTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGAc                                                      >  1:6917803/1‑93 (MQ=255)
                                       cGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATga                                                  <  1:1726644/93‑1 (MQ=255)
                                         tGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATgaga                                                <  1:42941/93‑1 (MQ=255)
                                           ccGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATgagaga                                              <  1:1101026/93‑1 (MQ=255)
                                           ccGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATgagaga                                              <  2:1285478/93‑1 (MQ=255)
                                            cGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGAc                                             <  2:6936178/93‑1 (MQ=255)
                                             ggACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACg                                            <  1:4492732/93‑1 (MQ=255)
                                              gACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGt                                           <  2:2667804/93‑1 (MQ=255)
                                               aCGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTc                                          <  1:4167963/93‑1 (MQ=255)
                                                  ttttGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGa                                       >  1:403085/1‑93 (MQ=255)
                                                   tttGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGaa                                      <  2:4228908/93‑1 (MQ=255)
                                                   tttGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGaa                                      <  2:3203844/93‑1 (MQ=255)
                                                    ttGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAg                                     <  1:5749165/93‑1 (MQ=255)
                                                     tGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGc                                    <  1:7704787/93‑1 (MQ=255)
                                                     tGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGc                                    <  2:5589086/93‑1 (MQ=255)
                                                     tGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGc                                    >  2:7469300/1‑93 (MQ=255)
                                                      gTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGcc                                   >  1:5085499/1‑93 (MQ=255)
                                                       ttCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCg                                  <  2:3924376/93‑1 (MQ=255)
                                                       ttCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCg                                  >  2:7352841/1‑93 (MQ=255)
                                                        tCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCgg                                 <  1:7255952/93‑1 (MQ=255)
                                                        tCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCgg                                 >  2:7134278/1‑93 (MQ=255)
                                                         cATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGt                                >  2:519815/1‑93 (MQ=255)
                                                         cATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGt                                <  1:6098316/93‑1 (MQ=255)
                                                          aTTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTc                               <  2:4515248/93‑1 (MQ=255)
                                                            tAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCtt                             <  1:6518093/93‑1 (MQ=255)
                                                            tAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCtt                             >  1:3188692/1‑93 (MQ=255)
                                                             aaaaTACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCttt                            <  2:6408660/93‑1 (MQ=255)
                                                                 tACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTcc                        <  2:4873439/93‑1 (MQ=255)
                                                                 tACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTcc                        <  1:2700765/93‑1 (MQ=255)
                                                                 tACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTcc                        <  2:1788568/93‑1 (MQ=255)
                                                                    tcactcGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGc                     >  2:6539210/1‑93 (MQ=255)
                                                                    tcactcGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGc                     >  1:351221/1‑93 (MQ=255)
                                                                    tcactcGACTCTGCTCATTCAGGGGCGAGGAAAACTTACTTATTCTGACGCTGTGATGACATGAGCGACGTCAGAAGCCGGTCTTTTTCCTgt                     >  1:6708723/1‑92 (MQ=255)
                                                                       ctcGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCcac                  <  1:5386018/93‑1 (MQ=255)
                                                                        tcGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCcaca                 >  2:3026422/1‑93 (MQ=255)
                                                                         cGCCTCTGTTAATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACaa                <  2:7220541/93‑1 (MQ=255)
                                                                         cGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGCCGTCAGAAGCCGGTCTTTTCCCTGCCACaa                <  2:2474566/93‑1 (MQ=255)
                                                                         cGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACaa                <  2:5268259/93‑1 (MQ=255)
                                                                         cGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACaa                <  1:2587384/93‑1 (MQ=255)
                                                                          gCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAt               >  1:7431249/1‑93 (MQ=255)
                                                                          gCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAt               >  1:6810829/1‑93 (MQ=255)
                                                                           ccTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATg              >  1:2301381/1‑93 (MQ=255)
                                                                           ccTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATg              >  1:1487081/1‑93 (MQ=255)
                                                                           ccTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATg              >  1:2067374/1‑93 (MQ=255)
                                                                            ctctGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGa             >  2:6203689/1‑93 (MQ=255)
                                                                            ctctGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGa             >  2:7612212/1‑93 (MQ=255)
                                                                             tctGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAg            >  2:2060361/1‑93 (MQ=255)
                                                                             tctGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAg            >  1:7756696/1‑93 (MQ=255)
                                                                             tctGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAg            >  2:6697263/1‑93 (MQ=255)
                                                                             cctGCTCATCCAGGGGCGAGTAAACCTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAg            <  1:64009/92‑1 (MQ=255)
                                                                              ctGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGt           <  2:5706462/93‑1 (MQ=255)
                                                                               tGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGCCGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGtt          >  1:4429810/1‑93 (MQ=255)
                                                                               tGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGtt          <  2:1420857/93‑1 (MQ=255)
                                                                               tGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGtt          >  1:3591555/1‑93 (MQ=255)
                                                                               tGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGtt          >  2:6048773/1‑93 (MQ=255)
                                                                               tGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGACGGCCTTTTTCCTGCCACAATGAGtt          >  1:2373764/1‑93 (MQ=255)
                                                                                gCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTc         <  1:6325470/93‑1 (MQ=255)
                                                                                gCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTc         <  1:5561066/93‑1 (MQ=255)
                                                                                 cTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCa        >  1:4712000/1‑93 (MQ=255)
                                                                                 cTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCa        <  1:5174993/93‑1 (MQ=255)
                                                                                   cATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGc      >  1:3843480/1‑93 (MQ=255)
                                                                                   cATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGc      <  2:1178813/93‑1 (MQ=255)
                                                                                      tCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGCCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCAt   <  2:2155006/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATT  >  NC_000913/4043147‑4043326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 251 total aligned reads displayed.
GTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTG  >  NC_000913/4043144‑4043330
                                                                                             |                                                                                             
GTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGG                                                                                               <  SRR4302543.2089100/94‑1 (MQ=60)
GTTTTTAACGGACAGGAGAAGAGCGAAGTAGAAATTATGTCACGTACCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGG                                                                                               <  SRR4302543.7087998/94‑1 (MQ=60)
 TTTTTATCGGACAGGAGAGGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGG                                                                                              <  SRR4302543.514832/94‑1 (MQ=60)
 TTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGG                                                                                              <  SRR4302543.7795669/94‑1 (MQ=60)
 TTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGG                                                                                              <  SRR4302543.514830/94‑1 (MQ=60)
  TTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCANTCGCCTCTGCTCCTNCAGGGG                                                                                             >  SRR4302543.1338954/1‑94 (MQ=60)
  TTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGG                                                                                             <  SRR4302543.5431020/94‑1 (MQ=60)
  TTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGG                                                                                             <  SRR4302543.7234593/94‑1 (MQ=60)
  TTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGG                                                                                             <  SRR4302543.5431021/94‑1 (MQ=60)
   TTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGC                                                                                            <  SRR4302543.5280870/94‑1 (MQ=60)
   TTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGC                                                                                            >  SRR4302543.7268636/1‑94 (MQ=60)
    TTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCG                                                                                           <  SRR4302543.5003949/94‑1 (MQ=60)
     TAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGA                                                                                          <  SRR4302543.3912211/94‑1 (MQ=60)
     TAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGA                                                                                          <  SRR4302543.4381847/94‑1 (MQ=60)
     TAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGA                                                                                          <  SRR4302543.7253611/94‑1 (MQ=60)
      AACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAG                                                                                         <  SRR4302543.1237237/94‑1 (MQ=60)
      AACGGACAGGAGATGAGCGAAGAAGAAGTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCTCTCGCCTCTGCTCATTCAGGGGCGAG                                                                                         <  SRR4302543.4370583/94‑1 (MQ=60)
       ACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGT                                                                                        <  SRR4302543.1477650/94‑1 (MQ=60)
        CGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTA                                                                                       >  SRR4302543.7302037/1‑94 (MQ=60)
        CGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTA                                                                                       <  SRR4302543.5676538/94‑1 (MQ=60)
        CGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTA                                                                                       <  SRR4302543.6298309/94‑1 (MQ=60)
         GGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAA                                                                                      <  SRR4302543.411179/94‑1 (MQ=60)
           ACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAA                                                                                    <  SRR4302543.4671774/94‑1 (MQ=60)
           ACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAA                                                                                    >  SRR4302543.396996/1‑94 (MQ=60)
            CAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCCGGGGCGAGTAAAAC                                                                                   >  SRR4302543.3314826/1‑94 (MQ=60)
             AGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACT                                                                                  >  SRR4302543.7557125/1‑94 (MQ=60)
             AGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACT                                                                                  <  SRR4302543.5454739/94‑1 (MQ=60)
              GGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTT                                                                                 >  SRR4302543.882155/1‑94 (MQ=60)
               GAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTA                                                                                <  SRR4302543.6939128/94‑1 (MQ=60)
               GAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTA                                                                                >  SRR4302543.3252993/1‑94 (MQ=60)
                 GATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACT                                                                              <  SRR4302543.5583444/94‑1 (MQ=60)
                 GATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACT                                                                              <  SRR4302543.1214593/94‑1 (MQ=60)
                  ATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTT                                                                             <  SRR4302543.572211/94‑1 (MQ=60)
                  ATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTT                                                                             <  SRR4302543.1881255/94‑1 (MQ=60)
                  ATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTT                                                                             >  SRR4302543.5870758/1‑94 (MQ=60)
                  ATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTT                                                                             >  SRR4302543.5086839/1‑94 (MQ=60)
                   TGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTA                                                                            >  SRR4302543.3939973/1‑94 (MQ=60)
                   TGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTA                                                                            >  SRR4302543.2192929/1‑94 (MQ=60)
                    GAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTAT                                                                           <  SRR4302543.7577242/94‑1 (MQ=60)
                    GAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTAT                                                                           <  SRR4302543.5481996/94‑1 (MQ=60)
                     ATCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATT                                                                          <  SRR4302543.5090827/94‑1 (MQ=60)
                     AGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATT                                                                          <  SRR4302543.3848468/94‑1 (MQ=60)
                     AGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATT                                                                          >  SRR4302543.3486599/1‑94 (MQ=60)
                     AGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATT                                                                          >  SRR4302543.4883546/1‑94 (MQ=60)
                      GCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTC                                                                         <  SRR4302543.1431639/94‑1 (MQ=60)
                      GCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTC                                                                         <  SRR4302543.3027411/94‑1 (MQ=60)
                       CGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTTATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAATAAAACTTACTTATTCT                                                                        >  SRR4302543.6533833/1‑94 (MQ=60)
                       CGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCT                                                                        <  SRR4302543.3255885/94‑1 (MQ=60)
                       CGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCT                                                                        >  SRR4302543.6298304/1‑94 (MQ=60)
                       CGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCT                                                                        >  SRR4302543.438299/1‑94 (MQ=60)
                       CGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCT                                                                        >  SRR4302543.3538391/1‑94 (MQ=60)
                        GAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTG                                                                       <  SRR4302543.2474659/94‑1 (MQ=60)
                        GAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTG                                                                       >  SRR4302543.4939129/1‑94 (MQ=60)
                        GAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGGTCATTAAAATACTAACTCGCCTCTGCTCATTCAGGGGCGAGGAAAACTTACTTATTCTG                                                                       >  SRR4302543.4939127/1‑94 (MQ=60)
                         AAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGA                                                                      >  SRR4302543.3246299/1‑94 (MQ=60)
                         AAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGA                                                                      >  SRR4302543.7157750/1‑94 (MQ=60)
                         AAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGCGGCGAGTAAAACTTACTTATTCTGC                                                                      >  SRR4302543.2181412/1‑94 (MQ=60)
                          AGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGAC                                                                     >  SRR4302543.7448362/1‑94 (MQ=60)
                          AGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGAC                                                                     <  SRR4302543.7557684/94‑1 (MQ=60)
                          AGAAGAATTTATGTCTCGTGCCGGACGTTGTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGAC                                                                     <  SRR4302543.4216125/94‑1 (MQ=60)
                            AAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGC                                                                   >  SRR4302543.3724519/1‑94 (MQ=60)
                             AGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCT                                                                  <  SRR4302543.1156532/94‑1 (MQ=60)
                             AGAATTTATGTCTCGTGCCGGACGTTTTGTCCATTAAAATACTCCCTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCT                                                                  <  SRR4302543.1156325/94‑1 (MQ=60)
                              GAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTG                                                                 <  SRR4302543.5480939/94‑1 (MQ=60)
                               AATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGT                                                                <  SRR4302543.7801830/94‑1 (MQ=60)
                                ATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCCCTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTG                                                               <  SRR4302543.6245847/94‑1 (MQ=60)
                                ATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTG                                                               <  SRR4302543.456715/94‑1 (MQ=60)
                                ATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTG                                                               >  SRR4302543.4810394/1‑94 (MQ=60)
                                 TTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGA                                                              >  SRR4302543.6821845/1‑94 (MQ=60)
                                  TTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGAT                                                             <  SRR4302543.4544381/94‑1 (MQ=60)
                                  TTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGAT                                                             <  SRR4302543.4299840/94‑1 (MQ=60)
                                  TTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGAT                                                             <  SRR4302543.1549761/94‑1 (MQ=60)
                                  TTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGAT                                                             >  SRR4302543.3212286/1‑94 (MQ=60)
                                  TTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAAATTACTTATTCTGACGCTGTGAT                                                             >  SRR4302543.5704768/1‑94 (MQ=60)
                                  TTATGTCTCGTGCCGGACGTTGTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGAT                                                             <  SRR4302543.1549762/94‑1 (MQ=60)
                                   TATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATG                                                            <  SRR4302543.3856024/94‑1 (MQ=60)
                                   TATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATG                                                            >  SRR4302543.6052703/1‑94 (MQ=60)
                                   TATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATG                                                            >  SRR4302543.2862840/1‑94 (MQ=60)
                                   TATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATG                                                            <  SRR4302543.2987140/94‑1 (MQ=60)
                                   TATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATG                                                            <  SRR4302543.5728856/94‑1 (MQ=60)
                                     TGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGAC                                                          >  SRR4302543.6982838/1‑94 (MQ=60)
                                      GTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACA                                                         <  SRR4302543.644197/94‑1 (MQ=60)
                                      GTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCCCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACA                                                         >  SRR4302543.7848075/1‑94 (MQ=60)
                                       TCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAATTTACTTATTCTGACGCTGTGATGACAT                                                        <  SRR4302543.2681923/94‑1 (MQ=60)
                                       TCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACAT                                                        <  SRR4302543.1364579/94‑1 (MQ=60)
                                       TCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACAT                                                        <  SRR4302543.3630036/94‑1 (MQ=60)
                                       TCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACAT                                                        <  SRR4302543.1879938/94‑1 (MQ=60)
                                        CTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATG                                                       <  SRR4302543.1618791/94‑1 (MQ=60)
                                        CTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATG                                                       >  SRR4302543.1520389/1‑94 (MQ=60)
                                         TCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGA                                                      <  SRR4302543.2358477/94‑1 (MQ=60)
                                          CGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAG                                                     <  SRR4302543.4361363/94‑1 (MQ=60)
                                          CGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAG                                                     <  SRR4302543.1742033/94‑1 (MQ=60)
                                           GTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGA                                                    >  SRR4302543.2486187/1‑94 (MQ=60)
                                           GTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGA                                                    >  SRR4302543.5736125/1‑94 (MQ=60)
                                            TGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAG                                                   <  SRR4302543.43334/94‑1 (MQ=60)
                                            TGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAG                                                   <  SRR4302543.2348373/94‑1 (MQ=60)
                                             GCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTATAACTTACTTATTCTGACGCTGTGATGACATGAGAGA                                                  >  SRR4302543.4957137/1‑94 (MQ=60)
                                             GCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGA                                                  >  SRR4302543.6534427/1‑94 (MQ=60)
                                              CCGGACGTTTTGTTCATTAAAATTCTCCCTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTGTGACGCTGTGATGACATGAGAGAC                                                 <  SRR4302543.207277/94‑1 (MQ=60)
                                              CCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGAC                                                 <  SRR4302543.1110943/94‑1 (MQ=60)
                                              CCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGAC                                                 <  SRR4302543.5866475/94‑1 (MQ=60)
                                              CCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGAC                                                 >  SRR4302543.5024442/1‑94 (MQ=60)
                                               CGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACG                                                >  SRR4302543.69458/1‑94 (MQ=60)
                                                GGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGT                                               >  SRR4302543.6717962/1‑94 (MQ=60)
                                                GGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGT                                               >  SRR4302543.6869215/1‑94 (MQ=60)
                                                GGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGT                                               <  SRR4302543.2735273/94‑1 (MQ=60)
                                                GGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGT                                               <  SRR4302543.4533559/94‑1 (MQ=60)
                                                  ACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCA                                             <  SRR4302543.4205709/94‑1 (MQ=60)
                                                   CGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTCTTCTGACGCTGTGATGACATGAGAGACGTCAG                                            >  SRR4302543.5156659/1‑94 (MQ=60)
                                                   CGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAG                                            >  SRR4302543.6807768/1‑94 (MQ=60)
                                                   CGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAG                                            <  SRR4302543.2680250/94‑1 (MQ=60)
                                                   CGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAG                                            <  SRR4302543.2102957/94‑1 (MQ=60)
                                                   CGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAG                                            <  SRR4302543.5284474/94‑1 (MQ=60)
                                                    GTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGA                                           >  SRR4302543.6216498/1‑94 (MQ=60)
                                                    GTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGA                                           >  SRR4302543.220264/1‑94 (MQ=60)
                                                    GTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGA                                           >  SRR4302543.562620/1‑94 (MQ=60)
                                                    GTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGA                                           >  SRR4302543.4272389/1‑94 (MQ=60)
                                                    GTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGA                                           >  SRR4302543.406807/1‑94 (MQ=60)
                                                      TTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAG                                         <  SRR4302543.1188662/94‑1 (MQ=60)
                                                       TTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGC                                        <  SRR4302543.5801573/94‑1 (MQ=60)
                                                       TTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGC                                        <  SRR4302543.218059/94‑1 (MQ=60)
                                                        TGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCC                                       <  SRR4302543.6464396/94‑1 (MQ=60)
                                                        TGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCC                                       <  SRR4302543.7777685/94‑1 (MQ=60)
                                                        TGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCC                                       <  SRR4302543.7777684/94‑1 (MQ=60)
                                                        TGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCC                                       >  SRR4302543.1805154/1‑94 (MQ=60)
                                                         GTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCG                                      >  SRR4302543.7710148/1‑94 (MQ=60)
                                                         GTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCG                                      >  SRR4302543.2831253/1‑94 (MQ=60)
                                                          TTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGG                                     >  SRR4302543.4539267/1‑94 (MQ=60)
                                                          TTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGG                                     >  SRR4302543.1405328/1‑94 (MQ=60)
                                                          TTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGG                                     >  SRR4302543.4329837/1‑94 (MQ=60)
                                                          TTCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTATTTCGGACGCTGGGTTGACATGAGAGACGGCGGAAGCCGG                                     >  SRR4302543.425355/1‑94 (MQ=60)
                                                           TCATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGT                                    <  SRR4302543.7326095/94‑1 (MQ=60)
                                                            CATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTC                                   <  SRR4302543.6156095/94‑1 (MQ=60)
                                                             ATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCT                                  <  SRR4302543.6747973/94‑1 (MQ=60)
                                                             ATTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCT                                  <  SRR4302543.4698555/94‑1 (MQ=60)
                                                              TTAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTT                                 >  SRR4302543.3217449/1‑94 (MQ=60)
                                                               TAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTT                                >  SRR4302543.2385774/1‑94 (MQ=60)
                                                               TAAAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTT                                <  SRR4302543.6314578/94‑1 (MQ=60)
                                                                 AAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTT                              >  SRR4302543.3631330/1‑94 (MQ=60)
                                                                 AAATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTT                              >  SRR4302543.7171208/1‑94 (MQ=60)
                                                                  AATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCAGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTC                             <  SRR4302543.5154218/94‑1 (MQ=60)
                                                                   ATACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCC                            >  SRR4302543.78832/1‑94 (MQ=60)
                                                                    TACTCTCTCGCCTCTGCTCAGTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCT                           <  SRR4302543.6013449/94‑1 (MQ=60)
                                                                    TACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCT                           <  SRR4302543.2725262/94‑1 (MQ=60)
                                                                     ACTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTG                          >  SRR4302543.7298031/1‑94 (MQ=60)
                                                                      CTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGC                         >  SRR4302543.354468/1‑94 (MQ=60)
                                                                      CTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGC                         >  SRR4302543.6055571/1‑94 (MQ=60)
                                                                      CTCACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGC                         >  SRR4302543.2498873/1‑94 (MQ=60)
                                                                      CTCACTCGACTCTGCTCATTCAGGGGCGAGGAAAACTTACTTATTCTGACGCTGTGATGACATGAGCGACGTCAGAAGCCGGTCTTTTTCCTGT                         >  SRR4302543.6772072/1‑94 (MQ=60)
                                                                        CACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATTACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCC                       >  SRR4302543.4642880/1‑94 (MQ=60)
                                                                        CACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCA                       >  SRR4302543.870465/1‑94 (MQ=60)
                                                                        CACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCA                       >  SRR4302543.6158658/1‑94 (MQ=60)
                                                                        CACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCA                       <  SRR4302543.6388393/94‑1 (MQ=60)
                                                                         ACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCAC                      >  SRR4302543.4738271/1‑94 (MQ=60)
                                                                         ACTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCAC                      >  SRR4302543.4470477/1‑94 (MQ=60)
                                                                          CTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACA                     >  SRR4302543.6639908/1‑94 (MQ=60)
                                                                          CTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACA                     <  SRR4302543.3573956/94‑1 (MQ=60)
                                                                          CTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACA                     <  SRR4302543.3780875/94‑1 (MQ=60)
                                                                          CTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACA                     <  SRR4302543.3780876/94‑1 (MQ=60)
                                                                          CTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACA                     <  SRR4302543.5434932/94‑1 (MQ=60)
                                                                          CTCGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACA                     <  SRR4302543.5434931/94‑1 (MQ=60)
                                                                            CGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAT                   <  SRR4302543.2610914/94‑1 (MQ=60)
                                                                            CGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAT                   >  SRR4302543.7502496/1‑94 (MQ=60)
                                                                            CGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAT                   >  SRR4302543.7502495/1‑94 (MQ=60)
                                                                            CGCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAAT                   >  SRR4302543.6875133/1‑94 (MQ=60)
                                                                             GCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGGTGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATG                  <  SRR4302543.5503701/94‑1 (MQ=60)
                                                                             GCCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATG                  >  SRR4302543.2085857/1‑94 (MQ=60)
                                                                              CCTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGA                 <  SRR4302543.6069557/94‑1 (MQ=60)
                                                                               CTCTGCTCATTCAGGGGCGAGTAAAGCTTACTTATTCTGACGGTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAG                <  SRR4302543.1636133/94‑1 (MQ=60)
                                                                               CTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAG                >  SRR4302543.7829909/1‑94 (MQ=60)
                                                                               CTCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAG                >  SRR4302543.2021583/1‑94 (MQ=60)
                                                                                TCTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGT               <  SRR4302543.64573/94‑1 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGCCGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.4469940/1‑94 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.5208602/1‑94 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.4469941/1‑94 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              <  SRR4302543.3563445/94‑1 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.3623261/1‑94 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.4348431/1‑94 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.4162318/1‑94 (MQ=60)
                                                                                 CTGCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGACGGCCTTTTTCCTGCCACAATGAGTT              >  SRR4302543.2395273/1‑94 (MQ=60)
                                                                                   GCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCA            <  SRR4302543.5611639/94‑1 (MQ=60)
                                                                                   GCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCA            >  SRR4302543.4755059/1‑94 (MQ=60)
                                                                                   GCTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCA            <  SRR4302543.6384960/94‑1 (MQ=60)
                                                                                   GCTCATTCAGAGGCGAGTAAAACTTACTTATTCGGACGCTGTGATGAGAGGAGAGTCGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCA            <  SRR4302543.4919810/94‑1 (MQ=60)
                                                                                   GCTAATCCAGGGGCGAGTAAAACTTCCTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCA            <  SRR4302543.1771697/94‑1 (MQ=60)
                                                                                    CTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAG           <  SRR4302543.5221719/94‑1 (MQ=60)
                                                                                    CTCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAG           >  SRR4302543.6526243/1‑94 (MQ=60)
                                                                                    CTAATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAG           <  SRR4302543.1502512/94‑1 (MQ=60)
                                                                                     TCATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGC          >  SRR4302543.4415221/1‑94 (MQ=60)
                                                                                      CATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCC         <  SRR4302543.7821708/94‑1 (MQ=60)
                                                                                      CATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCC         <  SRR4302543.2107828/94‑1 (MQ=60)
                                                                                       ATTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTTTGATGACATGAGAGACGTCAGAAGCCGGACTTTTTCCTGCCACAATGAGTTCAGCCA        >  SRR4302543.1422857/1‑94 (MQ=60)
                                                                                        TTGAGGGGCGAGTAAACCTTTCTTATTCTGACGCTGTGATGACATGAGAGCCGTCAGAAGCCGGTCTTTTCCCTGCCACAATGAGTTCAGCCAT       <  SRR4302543.2234545/94‑1 (MQ=60)
                                                                                        TTCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCAT       <  SRR4302543.7387526/94‑1 (MQ=60)
                                                                                         TCAGGGGCGAGTAAACCTTACTTATTCTGCCGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATT      <  SRR4302543.4465164/94‑1 (MQ=60)
                                                                                         TCAGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATT      <  SRR4302543.4304055/94‑1 (MQ=60)
                                                                                           AGGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGC    >  SRR4302543.2229533/1‑94 (MQ=60)
                                                                                            GGGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCT   <  SRR4302543.156368/94‑1 (MQ=60)
                                                                                             GGGCGAGTAAACCTTACTTATTCTGACGCTGTGATGAAATGAGAGACGTCAGAACCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTG  <  SRR4302543.5730169/94‑1 (MQ=60)
                                                                                             GGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTG  >  SRR4302543.822575/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTTTTAACGGACAGGAGATGAGCGAAGAAGAATTTATGTCTCGTGCCGGACGTTTTGTTCATTAAAATACTCACTCGCCTCTGCTCATTCAGAGGCGAGTAAAACTTACTTATTCTGACGCTGTGATGACATGAGAGACGTCAGAAGCCGGTCTTTTTCCTGCCACAATGAGTTCAGCCATTGCTG  >  NC_000913/4043144‑4043330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑