Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,057,859 (T)9→8 intergenic (+97/‑167) yihK → / → yihL putative GTP‑binding factor/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,057,8510T.98.8% 1471.3 / 5.9 331intergenic (+89/‑175)yihK/yihLputative GTP‑binding factor/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base . (155/172);  total (157/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 384 total aligned reads displayed.

CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA  >  NC_000913/4057759‑4057939
                                                                                            |                                                                                        
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                         >  1:100692/1‑93 (MQ=255)
 tGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtt                                                                                         >  2:5745665/1‑93 (MQ=255)
  gACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttt                                                                                        >  2:439029/1‑93 (MQ=255)
   aCGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGtttt                                                                                       >  1:3040189/1‑93 (MQ=255)
      gAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAGAAGCTTCCAGCGATGGCAGGgtttttt                                                                                    >  2:3034813/1‑93 (MQ=255)
        aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                  >  2:133677/1‑92 (MQ=255)
        aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                  <  2:5045823/93‑2 (MQ=255)
        aaaCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttg                                                                                  <  2:2708352/93‑2 (MQ=255)
          aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                <  2:4697317/93‑4 (MQ=255)
          aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                <  1:4849414/93‑4 (MQ=255)
          aCGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcc                                                                                <  1:655286/93‑4 (MQ=255)
           cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                               <  1:4374867/93‑5 (MQ=255)
           cGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGttttttttgcct                                                                               <  1:6072985/93‑5 (MQ=255)
            gATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTg                                                                             >  2:2661310/1‑93 (MQ=255)
             aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGt                                                                            >  1:216353/1‑93 (MQ=255)
             aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGt                                                                            <  1:2017221/93‑1 (MQ=255)
             aTCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGt                                                                            >  2:7376004/1‑93 (MQ=255)
              tcgtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTc                                                                           <  2:4505827/93‑1 (MQ=255)
                gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGt                                                                         >  2:6012879/1‑93 (MQ=255)
                gtcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGt                                                                         >  1:3146986/1‑93 (MQ=255)
                 tcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTg                                                                        >  2:6100152/1‑93 (MQ=255)
                 tcgCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTg                                                                        >  2:6123188/1‑93 (MQ=255)
                  cgccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCGAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGa                                                                       <  1:5559942/93‑1 (MQ=255)
                   gccgcGCCACCCGCGCACCGAAAGACGATTAATTGCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAg                                                                      <  1:2358633/93‑1 (MQ=255)
                    ccgcGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgc                                                                     <  2:5297093/93‑1 (MQ=255)
                      gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcgc                                                                   >  2:2826591/1‑93 (MQ=255)
                      gcgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAgcgc                                                                   >  2:4575896/1‑93 (MQ=255)
                       cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGca                                                                  >  1:4034326/1‑93 (MQ=255)
                       cgcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGca                                                                  <  1:7410072/93‑1 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTATTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTGTTTGCCTGTCGTGAGCGcac                                                                 >  2:3157724/1‑93 (MQ=255)
                        gcCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGcac                                                                 <  1:6838442/93‑1 (MQ=255)
                          cAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCacac                                                               <  2:1860058/93‑1 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTACTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                              <  2:5639156/93‑1 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                              >  2:6980466/1‑93 (MQ=255)
                           aaCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAcc                                                              >  1:5346414/1‑93 (MQ=255)
                            aCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCa                                                             >  2:1603849/1‑93 (MQ=255)
                             ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                            <  2:2450032/93‑1 (MQ=255)
                             ccGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCaa                                                            <  1:3699253/93‑1 (MQ=255)
                               gcgcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATg                                                          <  1:7154514/93‑1 (MQ=255)
                                 gcACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCg                                                        >  1:860630/1‑93 (MQ=255)
                                   aCCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                      >  2:886929/1‑93 (MQ=255)
                                   aCCGAAAGACGATTAATTTCGTTTTTTAGTCAAAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAg                                                      <  1:3245622/93‑1 (MQ=255)
                                    ccGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGt                                                     <  1:3838789/93‑1 (MQ=255)
                                     cGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTa                                                    >  1:442063/1‑93 (MQ=255)
                                      gAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTT‑CCTGTCGTGAGCGCACACCAATGCGAGTAt                                                   >  2:7718179/1‑93 (MQ=255)
                                       aaaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtc                                                  >  2:7230713/1‑93 (MQ=255)
                                        aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct                                                 >  1:4545166/1‑93 (MQ=255)
                                        aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct                                                 >  2:5892156/1‑93 (MQ=255)
                                        aaGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCA‑GTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtct                                                 <  1:813632/93‑1 (MQ=255)
                                         aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                <  1:5674119/93‑1 (MQ=255)
                                         aGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                <  1:2720350/93‑1 (MQ=255)
                                         aGACGATAAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAtctc                                                <  1:4642551/93‑1 (MQ=255)
                                          gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                               >  2:3454935/1‑93 (MQ=255)
                                          gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                               >  2:3762458/1‑93 (MQ=255)
                                          gACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTcc                                               >  1:1424616/1‑93 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                            >  1:6164561/1‑93 (MQ=255)
                                             gATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCtt                                            <  2:126266/93‑1 (MQ=255)
                                              aTTAATTTCTTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTta                                           <  1:6730335/93‑1 (MQ=255)
                                               ttAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtat                                          >  2:13818/1‑93 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAGCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  2:5523294/93‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  2:6385328/93‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  1:5978426/93‑1 (MQ=255)
                                                tAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTtata                                         <  2:413451/93‑1 (MQ=255)
                                                  aTTTCGTTTTTTAGTCATAAAACACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                       <  1:34455/93‑1 (MQ=255)
                                                  aTTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAg                                       >  2:3482799/1‑93 (MQ=255)
                                                   tttCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGt                                      >  2:6662305/1‑93 (MQ=255)
                                                      cGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtg                                   >  2:2212030/1‑93 (MQ=255)
                                                       gTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCtgt                                  <  2:3922233/93‑1 (MQ=255)
                                                         tttttAGCCAAAAAAACCCTGCAAGCGATGGCA‑GTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTgg                                <  2:2750676/93‑1 (MQ=255)
                                                          ttttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGt                               >  1:1164142/1‑93 (MQ=255)
                                                            ttAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTGTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTa                             <  2:4827729/93‑1 (MQ=255)
                                                             tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                            <  2:1145321/93‑1 (MQ=255)
                                                             tAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAc                            <  2:744460/93‑1 (MQ=255)
                                                               gTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGt                          >  1:3643965/1‑93 (MQ=255)
                                                                tCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTc                         <  1:4716230/93‑1 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                       <  2:1690005/93‑1 (MQ=255)
                                                                  aTAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCaa                       >  1:5098622/1‑93 (MQ=255)
                                                                   tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                      >  1:6427072/1‑93 (MQ=255)
                                                                   tAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAt                      <  2:7588227/93‑1 (MQ=255)
                                                                    aaaaaaCCTGCCAGCGATGGTAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATa                     <  1:7094513/93‑1 (MQ=255)
                                                                     aaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                    >  1:5442028/1‑93 (MQ=255)
                                                                     aaaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATag                    >  1:2801841/1‑93 (MQ=255)
                                                                      aaaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATaga                   <  1:521496/93‑1 (MQ=255)
                                                                       aaaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagag                  <  2:3621407/93‑1 (MQ=255)
                                                                        aaCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATagaga                 <  1:6298902/93‑1 (MQ=255)
                                                                          ccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc               <  1:4799532/93‑1 (MQ=255)
                                                                          ccTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGc               <  2:6864499/93‑1 (MQ=255)
                                                                            tGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCtt             <  2:2554192/93‑1 (MQ=255)
                                                                             gCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCATATAAGTCTGTGGTTACGTCAATAGAGAGCttt            <  2:6784558/93‑1 (MQ=255)
                                                                                 gCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTca        >  1:2435526/1‑93 (MQ=255)
                                                                                   gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca      <  1:3001851/93‑1 (MQ=255)
                                                                                   gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca      >  2:289774/1‑93 (MQ=255)
                                                                                   gATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTcaca      >  1:7832746/1‑93 (MQ=255)
                                                                                    aTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa     >  1:1266402/1‑93 (MQ=255)
                                                                                    aTGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACaa     <  2:3721153/93‑1 (MQ=255)
                                                                                     tGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAt    >  1:1812370/1‑93 (MQ=255)
                                                                                       gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa  >  1:4192594/1‑93 (MQ=255)
                                                                                       gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa  <  2:5386749/93‑1 (MQ=255)
                                                                                       gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa  >  1:7625466/1‑93 (MQ=255)
                                                                                       gCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTa  <  2:3378959/93‑1 (MQ=255)
                                                                                            |                                                                                        
CTGACGGAAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA  >  NC_000913/4057759‑4057939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 206 total aligned reads displayed.
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCA  >  NC_000913/4057766‑4057951
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AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTG                                                                                              <  SRR4302543.980304/94‑1 (MQ=60)
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTG                                                                                              >  SRR4302543.7972195/1‑94 (MQ=60)
   ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCT                                                                                          <  SRR4302543.661288/94‑1 (MQ=60)
   ACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCT                                                                                          <  SRR4302543.4893637/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                         <  SRR4302543.3073904/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                         <  SRR4302543.3853722/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                         <  SRR4302543.6130541/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                         <  SRR4302543.1326420/94‑1 (MQ=60)
    CGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTG                                                                                         <  SRR4302543.4414502/94‑1 (MQ=60)
     GATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGT                                                                                        >  SRR4302543.218214/1‑94 (MQ=60)
     GATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGT                                                                                        >  SRR4302543.6164034/1‑94 (MQ=60)
     GATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGT                                                                                        >  SRR4302543.6149119/1‑94 (MQ=60)
      ATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTC                                                                                       <  SRR4302543.5414741/94‑1 (MQ=60)
      ATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTC                                                                                       <  SRR4302543.2035349/94‑1 (MQ=60)
        CGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGT                                                                                     >  SRR4302543.3175287/1‑94 (MQ=60)
        CGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGT                                                                                     >  SRR4302543.1274915/1‑94 (MQ=60)
          gcgcgGCGCCATCCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGA                                                                                   <  SRR4302543.3484945/89‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCGAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  <  SRR4302543.5610504/94‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  >  SRR4302543.5504970/1‑94 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  <  SRR4302543.95002/94‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  <  SRR4302543.5267590/94‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  <  SRR4302543.2078014/94‑1 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  >  SRR4302543.4863727/1‑94 (MQ=60)
           CGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAG                                                                                  <  SRR4302543.6110978/94‑1 (MQ=60)
            GCCGCGCCACCCGCGCACCGAAAGACGATTAATTGCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGC                                                                                 <  SRR4302543.2380022/94‑1 (MQ=60)
             CCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCT                                                                                >  SRR4302543.3066611/1‑94 (MQ=60)
             CCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCG                                                                                <  SRR4302543.7547031/94‑1 (MQ=60)
               GCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCA                                                                              >  SRR4302543.4070410/1‑94 (MQ=60)
               GCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCA                                                                              >  SRR4302543.4070409/1‑94 (MQ=60)
                CGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCAC                                                                             <  SRR4302543.7481175/94‑1 (MQ=60)
                 GCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACA                                                                            <  SRR4302543.6902989/94‑1 (MQ=60)
                  CCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACAC                                                                           >  SRR4302543.6029074/1‑94 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                          >  SRR4302543.5394791/1‑94 (MQ=60)
                   CAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACC                                                                          >  SRR4302543.399586/1‑94 (MQ=60)
                    AACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCA                                                                         >  SRR4302543.2890216/1‑94 (MQ=60)
                     ACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAA                                                                        >  SRR4302543.4547341/1‑94 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                       <  SRR4302543.3731775/94‑1 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                       <  SRR4302543.7389789/94‑1 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                       <  SRR4302543.6221814/94‑1 (MQ=60)
                      CCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAAT                                                                       <  SRR4302543.6517003/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                      <  SRR4302543.3839163/94‑1 (MQ=60)
                       CGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATG                                                                      >  SRR4302543.7694493/1‑94 (MQ=60)
                        GCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGC                                                                     <  SRR4302543.7224051/94‑1 (MQ=60)
                        GCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑‑TTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    >  SRR4302543.6362053/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    <  SRR4302543.1263062/94‑1 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    >  SRR4302543.868298/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    >  SRR4302543.4300014/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    >  SRR4302543.6241837/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    >  SRR4302543.484188/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    >  SRR4302543.868297/1‑94 (MQ=60)
                         CGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCA‑GTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCG                                                                    <  SRR4302543.4836551/94‑1 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGT                                                                 <  SRR4302543.3274974/94‑1 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGT                                                                 <  SRR4302543.828373/94‑1 (MQ=60)
                            ACCGAAAGACGATTAATTTCGTTTTTTAGTCAAAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGT                                                                 <  SRR4302543.3274973/94‑1 (MQ=60)
                            ACCGAAAGACAATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGT                                                                 <  SRR4302543.6120921/94‑1 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTGGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                >  SRR4302543.854215/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                <  SRR4302543.3188938/94‑1 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                >  SRR4302543.446196/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                <  SRR4302543.3872669/94‑1 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                >  SRR4302543.1508138/1‑94 (MQ=60)
                             CCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTA                                                                <  SRR4302543.3188937/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                               >  SRR4302543.4652260/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                               >  SRR4302543.470488/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                               <  SRR4302543.7938023/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                               >  SRR4302543.1019208/1‑94 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                               <  SRR4302543.5202382/94‑1 (MQ=60)
                              CGAAAGACGATTAATTTCGTTTTGTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTAT                                                               <  SRR4302543.4126459/94‑1 (MQ=60)
                               GAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATC                                                              >  SRR4302543.6251679/1‑94 (MQ=60)
                               GAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATC                                                              <  SRR4302543.1157624/94‑1 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                             >  SRR4302543.4586556/1‑94 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                             <  SRR4302543.3423638/94‑1 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                             >  SRR4302543.3089660/1‑94 (MQ=60)
                                AAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCT                                                             <  SRR4302543.187630/94‑1 (MQ=60)
                                 AAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTC                                                            >  SRR4302543.6914268/1‑94 (MQ=60)
                                 AAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCA‑GTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTC                                                            <  SRR4302543.820793/94‑1 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                           >  SRR4302543.1437511/1‑94 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                           <  SRR4302543.2745085/94‑1 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                           >  SRR4302543.394032/1‑94 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                           <  SRR4302543.5352292/94‑1 (MQ=60)
                                  AGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                           <  SRR4302543.5725913/94‑1 (MQ=60)
                                  AGACGATAAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCC                                                           <  SRR4302543.4684785/94‑1 (MQ=60)
                                   GACGATTAATTTCTTTTTTTAGTCAGAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCC                                                          <  SRR4302543.5881900/94‑1 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGTCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                        >  SRR4302543.6222898/1‑94 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                        >  SRR4302543.7880449/1‑94 (MQ=60)
                                     CGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTT                                                        >  SRR4302543.2968245/1‑94 (MQ=60)
                                      GATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTA                                                       >  SRR4302543.2625935/1‑94 (MQ=60)
                                       ATTAATTTCTTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                      <  SRR4302543.6793914/94‑1 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                      >  SRR4302543.6303900/1‑94 (MQ=60)
                                       ATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                      >  SRR4302543.2649358/1‑94 (MQ=60)
                                       ATGAATTTCGTTTTTTAGTCATAAAAAGCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTAT                                                      <  SRR4302543.5180088/94‑1 (MQ=60)
                                         TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                    >  SRR4302543.1248479/1‑94 (MQ=60)
                                         TAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAA                                                    <  SRR4302543.6033190/94‑1 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                   <  SRR4302543.4377036/94‑1 (MQ=60)
                                          AATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAG                                                   <  SRR4302543.2144870/94‑1 (MQ=60)
                                           ATTTCGTTTTTTAGTCATAAAACACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGT                                                  <  SRR4302543.34770/94‑1 (MQ=60)
                                            TTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTC                                                 >  SRR4302543.4870764/1‑94 (MQ=60)
                                            TTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCGGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTC                                                 <  SRR4302543.5111715/94‑1 (MQ=60)
                                              TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCT                                                >  SRR4302543.7419043/1‑94 (MQ=60)
                                              TCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTG                                               <  SRR4302543.4877940/94‑1 (MQ=60)
                                               CGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGT                                              <  SRR4302543.1323012/94‑1 (MQ=60)
                                               CGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGT                                              >  SRR4302543.7014987/1‑94 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTG                                             <  SRR4302543.3533155/94‑1 (MQ=60)
                                                GTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTA                                             <  SRR4302543.6874425/94‑1 (MQ=60)
                                                 TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                            <  SRR4302543.3767437/94‑1 (MQ=60)
                                                 TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                            <  SRR4302543.7938458/94‑1 (MQ=60)
                                                 TTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGG                                            >  SRR4302543.2329663/1‑94 (MQ=60)
                                                  TTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGT                                           >  SRR4302543.1174736/1‑94 (MQ=60)
                                                   TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                          >  SRR4302543.5422522/1‑94 (MQ=60)
                                                   TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                          <  SRR4302543.6941779/94‑1 (MQ=60)
                                                   TTTTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTT                                          >  SRR4302543.4441242/1‑94 (MQ=60)
                                                     TTAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTAC                                        <  SRR4302543.6501100/94‑1 (MQ=60)
                                                      TAGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACG                                       <  SRR4302543.2745999/94‑1 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                      >  SRR4302543.3676009/1‑94 (MQ=60)
                                                       AGTCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGT                                      >  SRR4302543.3046535/1‑94 (MQ=60)
                                                         TCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCA                                    <  SRR4302543.4759319/94‑1 (MQ=60)
                                                         TCATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCA                                    <  SRR4302543.788743/94‑1 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                   >  SRR4302543.5144788/1‑94 (MQ=60)
                                                          CATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAA                                   <  SRR4302543.2417961/94‑1 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                  >  SRR4302543.6487632/1‑94 (MQ=60)
                                                           ATAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAAT                                  <  SRR4302543.5727221/94‑1 (MQ=60)
                                                            TAAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATA                                 <  SRR4302543.4613741/94‑1 (MQ=60)
                                                             AAAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                <  SRR4302543.4558117/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGTAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                <  SRR4302543.7163407/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGTAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                <  SRR4302543.7163408/94‑1 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                >  SRR4302543.5491427/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                >  SRR4302543.2827463/1‑94 (MQ=60)
                                                             AAAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAG                                <  SRR4302543.568094/94‑1 (MQ=60)
                                                              AAAACCCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                               <  SRR4302543.1769299/94‑1 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                               >  SRR4302543.6701545/1‑94 (MQ=60)
                                                              AAAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGA                               >  SRR4302543.2749221/1‑94 (MQ=60)
                                                               AAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                              <  SRR4302543.6822133/94‑1 (MQ=60)
                                                               AAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                              <  SRR4302543.526334/94‑1 (MQ=60)
                                                               AAAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAG                              <  SRR4302543.526335/94‑1 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                             >  SRR4302543.4509288/1‑94 (MQ=60)
                                                                AAACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGA                             <  SRR4302543.5396711/94‑1 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                            >  SRR4302543.6076042/1‑94 (MQ=60)
                                                                 AACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAG                            <  SRR4302543.6358182/94‑1 (MQ=60)
                                                                  ACCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGC                           <  SRR4302543.3210939/94‑1 (MQ=60)
                                                                   CCTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCT                          <  SRR4302543.4843394/94‑1 (MQ=60)
                                                                    CTGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTT                         >  SRR4302543.7772902/1‑94 (MQ=60)
                                                                     TGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTT                        <  SRR4302543.2921372/94‑1 (MQ=60)
                                                                     TGCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTT                        >  SRR4302543.2529296/1‑94 (MQ=60)
                                                                      GCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTG                       <  SRR4302543.5254480/94‑1 (MQ=60)
                                                                      GCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTG                       <  SRR4302543.6558331/94‑1 (MQ=60)
                                                                      GCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTG                       >  SRR4302543.1780549/1‑94 (MQ=60)
                                                                      GCCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTG                       >  SRR4302543.5024538/1‑94 (MQ=60)
                                                                       CCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGT                      <  SRR4302543.7209842/94‑1 (MQ=60)
                                                                       CCAGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGCATCTCCCTTATAAGTCTAGGGTTACGTCAATAGAGCGCTTTGT                      >  SRR4302543.3407099/1‑94 (MQ=60)
                                                                         AGCGATGGGAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAGTGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                    <  SRR4302543.2060319/94‑1 (MQ=60)
                                                                         AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCCATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCC                    >  SRR4302543.492664/1‑94 (MQ=60)
                                                                         AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                    >  SRR4302543.2457499/1‑94 (MQ=60)
                                                                         AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                    >  SRR4302543.5714778/1‑94 (MQ=60)
                                                                         AGCGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCA                    >  SRR4302543.596357/1‑94 (MQ=60)
                                                                           CGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                  >  SRR4302543.7906492/1‑94 (MQ=60)
                                                                           CGATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACA                  <  SRR4302543.6468689/94‑1 (MQ=60)
                                                                            GATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAA                 >  SRR4302543.1277733/1‑94 (MQ=60)
                                                                            GATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAA                 <  SRR4302543.3029061/94‑1 (MQ=60)
                                                                             ATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAT                >  SRR4302543.2233172/1‑94 (MQ=60)
                                                                             ATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAT                <  SRR4302543.2820037/94‑1 (MQ=60)
                                                                             ATGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAAT                >  SRR4302543.1828622/1‑94 (MQ=60)
                                                                              TGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATT               <  SRR4302543.2391517/94‑1 (MQ=60)
                                                                              TGGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATT               <  SRR4302543.3728770/94‑1 (MQ=60)
                                                                               GGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA              >  SRR4302543.4448876/1‑94 (MQ=60)
                                                                               GGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA              >  SRR4302543.7697828/1‑94 (MQ=60)
                                                                               GGCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTA              >  SRR4302543.4230613/1‑94 (MQ=60)
                                                                                GCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT             <  SRR4302543.3696216/94‑1 (MQ=60)
                                                                                GCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT             <  SRR4302543.1870226/94‑1 (MQ=60)
                                                                                GCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT             <  SRR4302543.4611637/94‑1 (MQ=60)
                                                                                GCAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT             <  SRR4302543.1870225/94‑1 (MQ=60)
                                                                                 gcatgTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTAT             <  SRR4302543.1870227/89‑1 (MQ=60)
                                                                                 CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC            <  SRR4302543.2078409/94‑1 (MQ=60)
                                                                                 CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATC            <  SRR4302543.4115078/94‑1 (MQ=60)
                                                                                 CAGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGGCACGATTATC            >  SRR4302543.5138334/1‑94 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT           >  SRR4302543.1631458/1‑94 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT           >  SRR4302543.611442/1‑94 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT           <  SRR4302543.1911325/94‑1 (MQ=60)
                                                                                  AGG‑TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCT           >  SRR4302543.3745449/1‑94 (MQ=60)
                                                                                    agTTTTTTTTGCCTGTCGGGAGCGCACCCCAATGCCAGTAACCTCCTTATAAGTCTGTGGTTTATTCAATAGGGAGCTTTGTCACAATTATCTG          <  SRR4302543.3290987/92‑1 (MQ=38)
                                                                                    GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG          <  SRR4302543.917099/94‑1 (MQ=60)
                                                                                    GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG          >  SRR4302543.6515537/1‑94 (MQ=60)
                                                                                    GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG          <  SRR4302543.5459611/94‑1 (MQ=60)
                                                                                    GGTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTG          <  SRR4302543.2191498/94‑1 (MQ=60)
                                                                                     GTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGC         <  SRR4302543.3684762/94‑1 (MQ=60)
                                                                                      TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA        >  SRR4302543.1142106/1‑94 (MQ=60)
                                                                                      TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA        <  SRR4302543.3341432/94‑1 (MQ=60)
                                                                                      TTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCA        >  SRR4302543.6466755/1‑94 (MQ=60)
                                                                                       TTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAA       <  SRR4302543.4811071/94‑1 (MQ=60)
                                                                                        TTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAA      >  SRR4302543.7979918/1‑94 (MQ=60)
                                                                                         TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG     <  SRR4302543.3212994/94‑1 (MQ=60)
                                                                                         TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG     <  SRR4302543.6076260/94‑1 (MQ=60)
                                                                                         TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG     <  SRR4302543.2892617/94‑1 (MQ=60)
                                                                                         TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG     <  SRR4302543.5038448/94‑1 (MQ=60)
                                                                                         TTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAG     <  SRR4302543.2552095/94‑1 (MQ=60)
                                                                                          TTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGT    <  SRR4302543.4607840/94‑1 (MQ=60)
                                                                                          TTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGT    <  SRR4302543.5649848/94‑1 (MQ=60)
                                                                                           TTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTC   <  SRR4302543.4882079/94‑1 (MQ=60)
                                                                                           TTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTC   <  SRR4302543.1758375/94‑1 (MQ=60)
                                                                                           TTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTC   <  SRR4302543.6930884/94‑1 (MQ=60)
                                                                                            TTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCA  >  SRR4302543.7729441/1‑94 (MQ=60)
                                                                                            TTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCA  >  SRR4302543.7461807/1‑94 (MQ=60)
                                                                                             |                                                                                            
AAAACGATCGTCGCCGCGCCAACCGCGCACCGAAAGACGATTAATTTCGTTTTTTAGTCATAAAAAACCTGCCAGCGATGGCAGGTTTTTTTTTGCCTGTCGTGAGCGCACACCAATGCGAGTATCTCCCTTATAAGTCTGTGGTTACGTCAATAGAGAGCTTTGTCACAATTATCTGCAAAGTCA  >  NC_000913/4057766‑4057951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑