Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,161,702	C→G	A162G (GCA→GGA)	btuB →	BtuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,161,702	0	C	G	100.0%	889.7 / NA	277	A162G (GCA→GGA)	btuB	BtuB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (135/142); total (135/142)

Only 100 of 292 total aligned reads displayed.

GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT  >  NC_000913/4161612‑4161791

ggCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTcaggag                                                                                         <  1:2444541/93‑1 (MQ=255)

   cgcgCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAgggg                                                                                      >  2:5507137/1‑92 (MQ=255)

   cgcgCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGt                                                                                      <  1:5900833/93‑1 (MQ=255)

    gcgcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTg                                                                                     >  1:4398080/1‑93 (MQ=255)

      gcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                   <  1:5711787/93‑1 (MQ=255)

      gcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                   >  1:85485/1‑93 (MQ=255)

      gcTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTggg                                                                                   >  1:938223/1‑93 (MQ=255)

       cTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTgggg                                                                                  <  2:827997/93‑1 (MQ=255)

        tCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGa                                                                                 <  2:3764769/93‑1 (MQ=255)

        tCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGa                                                                                 >  2:2536714/1‑93 (MQ=255)

         ccGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGaa                                                                                <  2:4318221/93‑1 (MQ=255)

           gCTGTTTATGGTTCCGCTGCAATAGGCGGGGCGGTGAATATCATCACGACGCGCGATGAACCCCGAACGGAAATTTCAGGAGGGTGGGGAAga                                                                              >  2:7274046/1‑92 (MQ=255)

           gCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGc                                                                              >  1:4106419/1‑93 (MQ=255)

           gCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAga                                                                              >  1:6778325/1‑92 (MQ=255)

           gCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGc                                                                              >  1:4558648/1‑93 (MQ=255)

            cTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCa                                                                             <  2:6851652/93‑1 (MQ=255)

                 tATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGt                                                                        >  1:2227832/1‑93 (MQ=255)

                  aTGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGtt                                                                       <  1:1768570/93‑1 (MQ=255)

                  aTGGTTCCGATGCAATAGGCGGGGGGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGtt                                                                       >  2:40494/1‑93 (MQ=255)

                   tGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTa                                                                      <  2:1744825/93‑1 (MQ=255)

                    ggTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTAt                                                                     <  2:1039026/93‑1 (MQ=255)

                     gTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATc                                                                    >  2:886470/1‑93 (MQ=255)

                          gATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAAc                                                               >  2:3422096/1‑93 (MQ=255)

                           aTGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACt                                                              >  1:6963300/1‑93 (MQ=255)

                           aTGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACt                                                              <  2:1498999/93‑1 (MQ=255)

                            tGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTa                                                             <  1:6344349/93‑1 (MQ=255)

                             gCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                            <  1:3869117/93‑1 (MQ=255)

                             gCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                            <  2:6665296/93‑1 (MQ=255)

                             gCAATAGGCGGGGTAGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTat                                                            <  1:3323921/93‑1 (MQ=255)

                               aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatga                                                          <  1:5158368/93‑1 (MQ=255)

                               aaTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatga                                                          <  1:7723932/93‑1 (MQ=255)

                                aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatgat                                                         <  1:1474996/93‑1 (MQ=255)

                                aTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTatgat                                                         <  1:721505/93‑1 (MQ=255)

                                aTAGGCGGGGTGGGGAATATCAGCACGCCGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTatgat                                                         <  1:5852146/93‑1 (MQ=255)

                                     cGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGtctc                                                    >  1:4635074/1‑93 (MQ=255)

                                      ggggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTctct                                                   <  2:5931442/93‑1 (MQ=255)

                                       gggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                  <  2:3350763/93‑1 (MQ=255)

                                       gggTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTa                                                  <  1:5532269/93‑1 (MQ=255)

                                        ggtggtGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGAGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTAc                                                 >  1:294968/1‑93 (MQ=255)

                                         gtggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACg                                                <  2:2932890/93‑1 (MQ=255)

                                          tggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgc                                               <  1:4983554/93‑1 (MQ=255)

                                          tggtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgc                                               >  1:2368833/1‑93 (MQ=255)

                                            gtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcag                                             >  2:2839354/1‑93 (MQ=255)

                                            gtgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcag                                             >  1:257616/1‑93 (MQ=255)

                                             tgAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcaga                                            >  1:6227731/1‑92 (MQ=255)

                                              gAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACgcagca                                           <  2:6759569/93‑1 (MQ=255)

                                               aaTATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaa                                          <  1:3252591/93‑1 (MQ=255)

                                               aaTATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaa                                          <  2:2567398/93‑1 (MQ=255)

                                               aaTATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaa                                          <  1:5926531/93‑1 (MQ=255)

                                                atatCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaac                                         <  2:3563093/93‑1 (MQ=255)

                                                  atcatcACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaacaa                                       <  1:67415/93‑1 (MQ=255)

                                                  atcatcACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGcaacaa                                       >  2:537297/1‑93 (MQ=255)

                                                   tcatcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCaacaac                                      >  2:6419301/1‑93 (MQ=255)

                                                    catcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACt                                     <  2:116628/93‑1 (MQ=255)

                                                    catcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACt                                     >  2:2037504/1‑93 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    <  2:6205341/93‑1 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    >  2:6977725/1‑93 (MQ=255)

                                                     atcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTg                                    >  2:1616533/1‑93 (MQ=255)

                                                      tcaCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgg                                   >  1:5251070/1‑93 (MQ=255)

                                                       caCGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTggg                                  >  2:7401503/1‑93 (MQ=255)

                                                        acgacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTgggg                                 >  2:3617376/1‑93 (MQ=255)

                                                         cgacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTggggg                                <  2:3989468/93‑1 (MQ=255)

                                                          gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                               <  2:4221856/93‑1 (MQ=255)

                                                          gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                               <  2:4929372/93‑1 (MQ=255)

                                                          gacgCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGa                               >  1:2117327/1‑93 (MQ=255)

                                                             gcgcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATaa                            <  2:2658735/93‑1 (MQ=255)

                                                             gcgcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATaa                            >  1:1128586/1‑93 (MQ=255)

                                                              cgcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAg                           <  2:2737429/93‑1 (MQ=255)

                                                              cgcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAg                           >  1:6419852/1‑93 (MQ=255)

                                                               gcgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGa                          >  1:7058448/1‑93 (MQ=255)

                                                                cgATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGac                         >  2:526805/1‑93 (MQ=255)

                                                                 gATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGaca                        <  1:1544479/93‑1 (MQ=255)

                                                                  aTGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGacac                       >  2:5326202/1‑93 (MQ=255)

                                                                    gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACgg                     <  1:340082/93‑1 (MQ=255)

                                                                    gAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACgg                     >  2:4536726/1‑93 (MQ=255)

                                                                     aaCCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACggg                    >  1:1406832/1‑93 (MQ=255)

                                                                       cccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTa                  >  2:3044964/1‑93 (MQ=255)

                                                                       cccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTa                  <  2:2199243/93‑1 (MQ=255)

                                                                        ccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACCCGGGTaa                 >  2:5853067/1‑93 (MQ=255)

                                                                        ccGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTaa                 >  1:6374458/1‑93 (MQ=255)

                                                                          ggaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGGTAAGACACGGGTAACg               >  1:3054917/1‑93 (MQ=255)

                                                                          ggaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACg               >  2:4735017/1‑93 (MQ=255)

                                                                          ggaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACg               >  2:6228835/1‑93 (MQ=255)

                                                                           gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc              <  1:1912720/93‑1 (MQ=255)

                                                                           gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc              >  2:3894429/1‑93 (MQ=255)

                                                                           gaacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc              <  2:1229532/93‑1 (MQ=255)

                                                                            aacggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCt             <  2:6052698/93‑1 (MQ=255)

                                                                             acggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg            >  2:7640864/1‑93 (MQ=255)

                                                                              cggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGt           <  1:2810339/93‑1 (MQ=255)

                                                                               ggaaATTTCAGGAGGGTGGGGAATCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGtt          <  1:56265/93‑1 (MQ=255)

                                                                               ggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGtt          <  1:4869854/93‑1 (MQ=255)

                                                                               ggaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATAATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGtt          <  2:1287830/93‑1 (MQ=255)

                                                                                gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTg         <  1:1022412/93‑1 (MQ=255)

                                                                                gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTg         <  1:1800892/93‑1 (MQ=255)

                                                                                gaaATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTg         <  1:5464523/93‑1 (MQ=255)

                                                                                 aaaTTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTgg        <  2:2596823/93‑1 (MQ=255)

                                                                                    tttCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGNCACGGGTAACGCTGTTGGGCg     >  1:319047/1‑93 (MQ=255)

                                                                                    tttCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCg     >  2:1401079/1‑93 (MQ=255)

                                                                                      tCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAt   <  1:3775850/93‑1 (MQ=255)

                                                                                       caggagGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGAtt  <  1:4323081/93‑1 (MQ=255)

GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT  >  NC_000913/4161612‑4161791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGC  >  NC_000913/4161610‑4161795

TGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGA                                                                                               <  SRR4302543.4855622/94‑1 (MQ=60)

TGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGA                                                                                               <  SRR4302543.6693318/94‑1 (MQ=60)

 GGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAG                                                                                              <  SRR4302543.2982550/94‑1 (MQ=60)

 GGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAG                                                                                              <  SRR4302543.6676516/94‑1 (MQ=60)

  GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGG                                                                                             <  SRR4302543.6399070/94‑1 (MQ=60)

  GGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGG                                                                                             <  SRR4302543.2466574/94‑1 (MQ=60)

   GCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGG                                                                                            <  SRR4302543.3328710/94‑1 (MQ=60)

   GCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGG                                                                                            >  SRR4302543.2532235/1‑94 (MQ=60)

   GCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGG                                                                                            <  SRR4302543.1820218/94‑1 (MQ=60)

   GCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGG                                                                                            <  SRR4302543.1745636/94‑1 (MQ=60)

    CCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACACGGAACGGAAATTTCAGGAGGGG                                                                                           >  SRR4302543.2944591/1‑94 (MQ=60)

     CGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTG                                                                                          >  SRR4302543.4437950/1‑94 (MQ=60)

     CGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTG                                                                                          <  SRR4302543.5954664/94‑1 (MQ=60)

     CGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTG                                                                                          >  SRR4302543.7803660/1‑94 (MQ=60)

       CGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGG                                                                                        >  SRR4302543.946420/1‑94 (MQ=60)

       CGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGG                                                                                        >  SRR4302543.86274/1‑94 (MQ=60)

       CGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGG                                                                                        <  SRR4302543.3874590/94‑1 (MQ=60)

        GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                       <  SRR4302543.3483134/94‑1 (MQ=60)

        GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                       <  SRR4302543.5749707/94‑1 (MQ=60)

        GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                       <  SRR4302543.1809053/94‑1 (MQ=60)

        GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                       <  SRR4302543.5763899/94‑1 (MQ=60)

        GCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGG                                                                                       >  SRR4302543.4865741/1‑94 (MQ=60)

         CTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGA                                                                                      >  SRR4302543.5049583/1‑94 (MQ=60)

         CTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGA                                                                                      >  SRR4302543.7529208/1‑94 (MQ=60)

           CCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAG                                                                                    >  SRR4302543.230040/1‑94 (MQ=60)

            CGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGC                                                                                   <  SRR4302543.7371089/94‑1 (MQ=60)

            CGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGC                                                                                   >  SRR4302543.4143358/1‑94 (MQ=60)

            CGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGC                                                                                   <  SRR4302543.414709/94‑1 (MQ=60)

            CGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGC                                                                                   >  SRR4302543.564290/1‑94 (MQ=60)

            CGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGC                                                                                   >  SRR4302543.4600151/1‑94 (MQ=60)

            CGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGA                                                                                   >  SRR4302543.6842345/1‑94 (MQ=60)

             GCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCA                                                                                  >  SRR4302543.2194178/1‑94 (MQ=60)

              CTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAA                                                                                 >  SRR4302543.5737348/1‑94 (MQ=60)

               TGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAAATTCAGGAGGGTGGGGGAGCAAT                                                                                >  SRR4302543.7023433/1‑94 (MQ=60)

                GTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATA                                                                               <  SRR4302543.2127782/94‑1 (MQ=60)

                 TTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAG                                                                              <  SRR4302543.3252256/94‑1 (MQ=60)

                  TTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGT                                                                             >  SRR4302543.342195/1‑94 (MQ=60)

                  TTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGT                                                                             >  SRR4302543.2247949/1‑94 (MQ=60)

                  TTATGGTTCCGATGCAATAGGCGGGGTGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGT                                                                             >  SRR4302543.5954921/1‑94 (MQ=60)

                   TATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTT                                                                            >  SRR4302543.1847661/1‑94 (MQ=60)

                    ATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTA                                                                           >  SRR4302543.4965752/1‑94 (MQ=60)

                    ATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTA                                                                           <  SRR4302543.1784382/94‑1 (MQ=60)

                     TGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTAT                                                                          <  SRR4302543.6039963/94‑1 (MQ=60)

                      GGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATC                                                                         >  SRR4302543.2873671/1‑94 (MQ=60)

                       GTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATAA                                                                        <  SRR4302543.7190011/94‑1 (MQ=60)

                        TTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAG                                                                       <  SRR4302543.5571213/94‑1 (MQ=60)

                        TTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAG                                                                       <  SRR4302543.451859/94‑1 (MQ=60)

                           CGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAAC                                                                    >  SRR4302543.7044021/1‑94 (MQ=60)

                           CGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAAC                                                                    >  SRR4302543.5665636/1‑94 (MQ=60)

                            GATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACT                                                                   >  SRR4302543.7028828/1‑94 (MQ=60)

                             ATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTA                                                                  >  SRR4302543.5963028/1‑94 (MQ=60)

                             ATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTT                                                                  >  SRR4302543.6940192/1‑94 (MQ=60)

                              gtCAATAGGCGGGGTGGGGAATATCATCACGACGGGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTAT                                                                 <  SRR4302543.2216015/92‑1 (MQ=60)

                              TGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGCAATTTCAGGAGGGTGGGGAAGCACTAGTTATCAGAACTAT                                                                 >  SRR4302543.6840629/1‑94 (MQ=60)

                              TGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTAT                                                                 <  SRR4302543.6404009/94‑1 (MQ=60)

                               GCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATG                                                                <  SRR4302543.3903284/94‑1 (MQ=60)

                               GCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATG                                                                <  SRR4302543.1082661/94‑1 (MQ=60)

                               GCAATAGGCGGGGTAGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATG                                                                <  SRR4302543.3353905/94‑1 (MQ=60)

                                CAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA                                                               <  SRR4302543.3976884/94‑1 (MQ=60)

                                 AATAGGCGGGGTGGTGAATATCATCATGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑T                                                             <  SRR4302543.2446954/94‑1 (MQ=60)

                                 AATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑T                                                             <  SRR4302543.7796956/94‑1 (MQ=60)

                                 AATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑T                                                             <  SRR4302543.5204987/94‑1 (MQ=60)

                                 AATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑T                                                             <  SRR4302543.5071011/94‑1 (MQ=60)

                                  ATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TG                                                            <  SRR4302543.5905398/94‑1 (MQ=60)

                                  ATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TG                                                            <  SRR4302543.7058207/94‑1 (MQ=60)

                                  ATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TG                                                            >  SRR4302543.1491494/1‑94 (MQ=60)

                                  ATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TG                                                            <  SRR4302543.1488285/94‑1 (MQ=60)

                                  ATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TG                                                            <  SRR4302543.728017/94‑1 (MQ=60)

                                  ATAGGCGGGGTGGGGAATATCAGCACGCCGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTATGA‑TG                                                            <  SRR4302543.5905397/94‑1 (MQ=60)

                                    AGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTC                                                          <  SRR4302543.1640950/94‑1 (MQ=60)

                                     GGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCT                                                         <  SRR4302543.3906434/94‑1 (MQ=60)

                                      GCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTC                                                        >  SRR4302543.4677217/1‑94 (MQ=60)

                                       CGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCT                                                       >  SRR4302543.5547154/1‑94 (MQ=60)

                                       CGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCT                                                       >  SRR4302543.5391260/1‑94 (MQ=60)

                                       CGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCT                                                       >  SRR4302543.6435821/1‑94 (MQ=60)

                                        GGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTA                                                      >  SRR4302543.5905801/1‑94 (MQ=60)

                                        GGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTA                                                      >  SRR4302543.6826233/1‑94 (MQ=60)

                                         GGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTAC                                                     <  SRR4302543.5582538/94‑1 (MQ=60)

                                         GGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTAC                                                     <  SRR4302543.2339506/94‑1 (MQ=60)

                                         GGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGAGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTAC                                                     >  SRR4302543.297611/1‑94 (MQ=60)

                                          GGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACG                                                    <  SRR4302543.4278515/94‑1 (MQ=60)

                                           GTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGC                                                   >  SRR4302543.1241817/1‑94 (MQ=60)

                                           GTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGC                                                   >  SRR4302543.2390303/1‑94 (MQ=60)

                                            TGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCA                                                  >  SRR4302543.5248049/1‑94 (MQ=60)

                                            TGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCA                                                  <  SRR4302543.5028915/94‑1 (MQ=60)

                                             GGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAG                                                 >  SRR4302543.259869/1‑94 (MQ=60)

                                             GGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCACGATCTATCAGCACTATGC‑TGTCTCGACGCAG                                                 >  SRR4302543.1658969/1‑94 (MQ=60)

                                              GTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGA                                                >  SRR4302543.6286519/1‑94 (MQ=60)

                                                 AATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAAC                                             <  SRR4302543.5980703/94‑1 (MQ=60)

                                                 AATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAAC                                             >  SRR4302543.2704482/1‑94 (MQ=60)

                                                 AATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAAC                                             <  SRR4302543.3281994/94‑1 (MQ=60)

                                                  ATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACA                                            >  SRR4302543.1464066/1‑94 (MQ=60)

                                                  ATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACA                                            >  SRR4302543.5773079/1‑94 (MQ=60)

                                                    ATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAAC                                          >  SRR4302543.7179781/1‑94 (MQ=60)

                                                    ATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAAC                                          <  SRR4302543.68016/94‑1 (MQ=60)

                                                      CATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTG                                        <  SRR4302543.1273220/94‑1 (MQ=60)

                                                      CATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTG                                        <  SRR4302543.4636981/94‑1 (MQ=60)

                                                      CATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGGGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTG                                        >  SRR4302543.2124054/1‑94 (MQ=60)

                                                       ATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGG                                       >  SRR4302543.5298411/1‑94 (MQ=60)

                                                        TCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGG                                      <  SRR4302543.2597492/94‑1 (MQ=60)

                                                        TCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGG                                      <  SRR4302543.3049425/94‑1 (MQ=60)

                                                        TCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGG                                      >  SRR4302543.3424544/1‑94 (MQ=60)

                                                        TCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGG                                      <  SRR4302543.6436513/94‑1 (MQ=60)

                                                        TCACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGG                                      <  SRR4302543.87033/94‑1 (MQ=60)

                                                         CACGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGG                                     <  SRR4302543.5889491/94‑1 (MQ=60)

                                                           CGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGA                                   >  SRR4302543.5385345/1‑94 (MQ=60)

                                                           CGACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGA                                   >  SRR4302543.2136281/1‑94 (MQ=60)

                                                            GACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGAT                                  <  SRR4302543.4599446/94‑1 (MQ=60)

                                                             ACGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATA                                 <  SRR4302543.6350187/94‑1 (MQ=60)

                                                              CGCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAA                                >  SRR4302543.1138829/1‑94 (MQ=60)

                                                               GCGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAG                               >  SRR4302543.6480336/1‑94 (MQ=60)

                                                                CGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGA                              >  SRR4302543.7126949/1‑94 (MQ=60)

                                                                CGCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGA                              >  SRR4302543.3831823/1‑94 (MQ=60)

                                                                CGCGATGAA‑CCGGAACGGAAATTTCAGGGGGGGGGGGAAGGAATAGTTATTAGAACTATGATTGTCT‑TACGCAGCAACAACTGGGGGATAAGAA                             >  SRR4302543.5815316/1‑94 (MQ=46)

                                                                 GCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGAC                             <  SRR4302543.3201232/94‑1 (MQ=60)

                                                                 GCGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGAC                             >  SRR4302543.3570403/1‑94 (MQ=60)

                                                                 GCGATGAACCCGGAACGGAAATTNCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGAC                             <  SRR4302543.3260771/94‑1 (MQ=60)

                                                                  CGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACA                            <  SRR4302543.4870171/94‑1 (MQ=60)

                                                                  CGATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACA                            >  SRR4302543.6406207/1‑94 (MQ=60)

                                                                   GATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACAC                           <  SRR4302543.1558309/94‑1 (MQ=60)

                                                                    ATGAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACG                          >  SRR4302543.180041/1‑94 (MQ=60)

                                                                      GAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGG                        <  SRR4302543.343212/94‑1 (MQ=60)

                                                                      GAACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGG                        >  SRR4302543.1419536/1‑94 (MQ=60)

                                                                        ACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAATAACTGGGGGATAAGACACGGGTA                      <  SRR4302543.959667/94‑1 (MQ=60)

                                                                        ACCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTA                      >  SRR4302543.6604244/1‑94 (MQ=60)

                                                                         CCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAA                     >  SRR4302543.6434421/1‑94 (MQ=60)

                                                                         CCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAA                     >  SRR4302543.891146/1‑94 (MQ=60)

                                                                         CCCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAA                     <  SRR4302543.3020540/94‑1 (MQ=60)

                                                                          CCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAC                    <  SRR4302543.6419044/94‑1 (MQ=60)

                                                                          CCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAC                    <  SRR4302543.441552/94‑1 (MQ=60)

                                                                          CCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAC                    <  SRR4302543.441553/94‑1 (MQ=60)

                                                                          CCGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAC                    <  SRR4302543.6697640/94‑1 (MQ=60)

                                                                           CGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGGTAAGACACGGGTAACG                   >  SRR4302543.3082426/1‑94 (MQ=60)

                                                                           CGGAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACG                   >  SRR4302543.4915772/1‑94 (MQ=60)

                                                                             GAACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCT                 <  SRR4302543.1929962/94‑1 (MQ=60)

                                                                               ACGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGT               <  SRR4302543.1493720/94‑1 (MQ=60)

                                                                                CGGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTT              <  SRR4302543.2836066/94‑1 (MQ=60)

                                                                                 GGAAATTTCAGGAGGGTGGGGAATCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTG             <  SRR4302543.56762/94‑1 (MQ=60)

                                                                                 GGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTG             <  SRR4302543.5268569/94‑1 (MQ=60)

                                                                                 GGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTG             >  SRR4302543.3098765/1‑94 (MQ=60)

                                                                                 GGAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTG             <  SRR4302543.4914246/94‑1 (MQ=60)

                                                                                  GAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGG            <  SRR4302543.1817018/94‑1 (MQ=60)

                                                                                  GAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGG            <  SRR4302543.5514042/94‑1 (MQ=60)

                                                                                  GAAATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGG            <  SRR4302543.1031462/94‑1 (MQ=60)

                                                                                    AATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGC          <  SRR4302543.3327257/94‑1 (MQ=60)

                                                                                     ATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGNCACGGGTAACGCTGTTGGGCG         >  SRR4302543.321933/1‑94 (MQ=60)

                                                                                     ATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCG         >  SRR4302543.1438623/1‑94 (MQ=60)

                                                                                     ATTTCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCG         <  SRR4302543.7899483/94‑1 (MQ=60)

                                                                                        TCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT      <  SRR4302543.3809098/94‑1 (MQ=60)

                                                                                        TCAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATT      <  SRR4302543.2444087/94‑1 (MQ=60)

                                                                                         CAGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTA     <  SRR4302543.4362333/94‑1 (MQ=60)

                                                                                          AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT    <  SRR4302543.146528/94‑1 (MQ=60)

                                                                                          AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT    <  SRR4302543.4809115/94‑1 (MQ=60)

                                                                                          AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT    <  SRR4302543.3140598/94‑1 (MQ=60)

                                                                                          AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT    <  SRR4302543.816175/94‑1 (MQ=60)

                                                                                          AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT    <  SRR4302543.50043/94‑1 (MQ=60)

                                                                                          AGGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAT    >  SRR4302543.6349851/1‑94 (MQ=60)

                                                                                           GGAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATG   <  SRR4302543.931459/94‑1 (MQ=60)

                                                                                            GAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGC  <  SRR4302543.6055554/94‑1 (MQ=60)

TGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTCAGCAGGGTGGGGAAGCAATAGTTATCAGAACTATGA‑TGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGC  >  NC_000913/4161610‑4161795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`