Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,382,443 A→G T357A (ACG→GCG)  yjeM → putative transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,382,4430AG99.5% 1209.4 / ‑4.5 394T357A (ACG→GCG) yjeMputative transport
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (209/181);  total (210/184)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 404 total aligned reads displayed.

GCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTAC  >  NC_000913/4382353‑4382534
                                                                                           |                                                                                           
 cgcgTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGgcgc                                                                                          <  2:1186879/93‑1 (MQ=255)
   cgTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCg                                                                                        <  2:7550688/93‑1 (MQ=255)
    gTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGACAGTCATCATCCA‑GGGGGCGCCGa                                                                                       >  2:774408/1‑93 (MQ=255)
    gTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGa                                                                                       >  2:2557407/1‑93 (MQ=255)
     tATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGaa                                                                                      >  2:5157367/1‑93 (MQ=255)
     tATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGaa                                                                                      >  2:6648069/1‑93 (MQ=255)
      aTTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGaaa                                                                                     >  2:5555181/1‑93 (MQ=255)
       ttACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAg                                                                                    >  2:6699515/1‑93 (MQ=255)
         aCTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCa                                                                                  <  2:6180541/93‑1 (MQ=255)
          cTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCAt                                                                                 <  2:4811321/93‑1 (MQ=255)
          cTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCAt                                                                                 >  1:3396198/1‑93 (MQ=255)
           tGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCAtt                                                                                >  2:2605149/1‑93 (MQ=255)
            ggACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATtg                                                                               >  2:4108202/1‑93 (MQ=255)
            ggACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATtg                                                                               <  2:2771774/93‑1 (MQ=255)
             gACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATtgt                                                                              <  1:6043724/93‑1 (MQ=255)
              aCTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATtgtg                                                                             <  1:7420679/93‑1 (MQ=255)
                tttCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGc                                                                           >  1:5082506/1‑93 (MQ=255)
                tttCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATCGTGGc                                                                           >  2:3849723/1‑93 (MQ=255)
                  tCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCg                                                                         >  2:3094548/1‑93 (MQ=255)
                    gATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGa                                                                       >  1:6720697/1‑93 (MQ=255)
                      tGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAAc                                                                     >  1:7121323/1‑93 (MQ=255)
                       gTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAAcc                                                                    >  2:738543/1‑93 (MQ=255)
                         tCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGa                                                                  <  2:2493775/93‑1 (MQ=255)
                            tCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGa                                                               <  2:1814245/93‑1 (MQ=255)
                             cGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGAc                                                              <  2:6099609/93‑1 (MQ=255)
                              gCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGAcg                                                             >  1:662041/1‑93 (MQ=255)
                               ccTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGAcgc                                                            >  2:4030008/1‑93 (MQ=255)
                                cTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGAcgcg                                                           >  1:4048384/1‑93 (MQ=255)
                                 tataCCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACgcgc                                                          <  2:4493061/93‑1 (MQ=255)
                                   taCCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCt                                                        >  1:7671203/1‑93 (MQ=255)
                                   taCCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCt                                                        >  1:955382/1‑93 (MQ=255)
                                   taCCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCt                                                        >  1:2734711/1‑93 (MQ=255)
                                      cGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGaa                                                     <  2:1013703/93‑1 (MQ=255)
                                       ggTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAAt                                                    <  2:568707/93‑1 (MQ=255)
                                        gTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATg                                                   >  1:5664912/1‑93 (MQ=255)
                                        gTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATg                                                   >  2:4664196/1‑93 (MQ=255)
                                        gTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATg                                                   <  1:3220162/93‑1 (MQ=255)
                                         tGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGCACCGATGACGCGCCTGAATgg                                                  >  2:2218959/1‑92 (MQ=255)
                                         tGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGc                                                  >  1:5853689/1‑93 (MQ=255)
                                          gCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCg                                                 >  2:3261470/1‑93 (MQ=255)
                                           ctttCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGa                                                <  1:2919968/91‑1 (MQ=255)
                                           cGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGa                                                <  2:6934551/93‑1 (MQ=255)
                                            gTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAt                                               >  2:2364297/1‑93 (MQ=255)
                                            gTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAt                                               >  2:5376600/1‑93 (MQ=255)
                                            gTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAt                                               >  2:83261/1‑93 (MQ=255)
                                            gTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAt                                               <  1:830260/93‑1 (MQ=255)
                                            gTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAt                                               <  1:1989421/93‑1 (MQ=255)
                                             ttCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATg                                              <  1:6817808/93‑1 (MQ=255)
                                                tttACGCTTTGCTATTCACAGTTGAAAGCCATCATCCA‑GGGGCCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATgggg                                           <  2:638698/93‑1 (MQ=255)
                                                 ttACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGa                                          >  1:1070279/1‑93 (MQ=255)
                                                 ttACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGa                                          <  2:3618258/93‑1 (MQ=255)
                                                 ttACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGa                                          >  1:4930559/1‑93 (MQ=255)
                                                   aCGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATg                                        >  1:3948712/1‑93 (MQ=255)
                                                   aCGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATg                                        >  2:3190864/1‑93 (MQ=255)
                                                    cGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGc                                       >  1:477384/1‑93 (MQ=255)
                                                      cTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCg                                     <  2:193167/93‑1 (MQ=255)
                                                      cTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCg                                     >  1:6232515/1‑93 (MQ=255)
                                                       tttGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCTCCTGAATGCGATGGGGATGCCGt                                    >  2:4364787/1‑93 (MQ=255)
                                                       tttGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGt                                    >  1:5440242/1‑93 (MQ=255)
                                                        ttGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTc                                   <  1:4324882/93‑1 (MQ=255)
                                                          gCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTa                                 >  1:5786490/1‑93 (MQ=255)
                                                          gCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTa                                 <  1:2302681/93‑1 (MQ=255)
                                                            tATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATc                               >  1:5907271/1‑93 (MQ=255)
                                                            tATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATc                               <  1:298797/93‑1 (MQ=255)
                                                            tATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATc                               <  2:6537650/93‑1 (MQ=255)
                                                             aTTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCg                              <  1:7821823/93‑1 (MQ=255)
                                                             aTTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCg                              >  2:1083754/1‑93 (MQ=255)
                                                              ttCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGc                             >  1:3706000/1‑93 (MQ=255)
                                                              ttCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGc                             <  2:6821007/93‑1 (MQ=255)
                                                                  ccGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtg                         >  1:3791033/1‑93 (MQ=255)
                                                                  ccGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtg                         <  2:2046072/93‑1 (MQ=255)
                                                                    gTTGAAAGCCATCATCCAGGGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtgt                        <  2:3707816/93‑1 (MQ=255)
                                                                    gTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtgtg                       >  1:2792661/1‑93 (MQ=255)
                                                                    gTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtgtg                       <  1:2575082/93‑1 (MQ=255)
                                                                    gTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtgtg                       >  1:7276831/1‑93 (MQ=255)
                                                                    gTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAtgtg                       >  2:493606/1‑93 (MQ=255)
                                                                     ttGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGCTGCCGTCTATCGCCATGTgg                      >  1:556783/1‑93 (MQ=255)
                                                                     ttGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTgg                      >  2:5842373/1‑93 (MQ=255)
                                                                      tGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGa                     <  2:1515342/93‑1 (MQ=255)
                                                                           gCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAg                <  1:3822696/93‑1 (MQ=255)
                                                                           gCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAg                <  2:1685637/93‑1 (MQ=255)
                                                                           gCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAg                >  1:6938987/1‑93 (MQ=255)
                                                                            ccATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGt               <  2:708281/93‑1 (MQ=255)
                                                                             catcatCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTg              >  1:2200278/1‑93 (MQ=255)
                                                                              atcatcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCATTGc             >  2:3533951/1‑93 (MQ=255)
                                                                              atcatcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGc             <  1:5141517/93‑1 (MQ=255)
                                                                               tcatcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCg            >  2:290980/1‑93 (MQ=255)
                                                                               tcatcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCg            <  1:4522888/93‑1 (MQ=255)
                                                                                 atcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCggg          >  1:1421924/1‑93 (MQ=255)
                                                                                 atcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCggg          <  2:4234865/93‑1 (MQ=255)
                                                                                  tcCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggt         >  1:1631041/1‑93 (MQ=255)
                                                                                    cA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttg       <  1:7188240/93‑1 (MQ=255)
                                                                                    cA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttg       >  2:2986298/1‑93 (MQ=255)
                                                                                    cA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttg       >  1:24141/1‑93 (MQ=255)
                                                                                    cA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttg       >  2:7650805/1‑93 (MQ=255)
                                                                                     a‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttgg      <  1:167544/93‑1 (MQ=255)
                                                                                       ggggGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttggt     >  2:7280941/1‑93 (MQ=255)
                                                                                       ggggGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttggt     <  2:5307239/93‑1 (MQ=255)
                                                                                        ggggCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttggtt    >  1:2447523/1‑93 (MQ=255)
                                                                                        ggggCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggttggtt    >  2:4734934/1‑93 (MQ=255)
                                                                                           |                                                                                           
GCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTAC  >  NC_000913/4382353‑4382534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTG  >  NC_000913/4382350‑4382536
                                                                                              |                                                                                             
TTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGG                                                                                               <  SRR4302543.7893797/94‑1 (MQ=60)
  TGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCG                                                                                             >  SRR4302543.854039/1‑94 (MQ=60)
    CGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCC                                                                                           >  SRR4302543.2418193/1‑94 (MQ=60)
       GTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAA                                                                                        >  SRR4302543.3814175/1‑94 (MQ=60)
         ATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAG                                                                                      >  SRR4302543.5897985/1‑94 (MQ=60)
         ATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAG                                                                                      <  SRR4302543.3420033/94‑1 (MQ=60)
           TACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCA                                                                                    <  SRR4302543.1681158/94‑1 (MQ=60)
            ACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCAT                                                                                   >  SRR4302543.3426637/1‑94 (MQ=60)
              TGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTG                                                                                 >  SRR4302543.2264580/1‑94 (MQ=60)
              TGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTG                                                                                 <  SRR4302543.3836380/94‑1 (MQ=60)
              TGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTG                                                                                 <  SRR4302543.3374075/94‑1 (MQ=60)
               TGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGACGCCGAAAGCATTGT                                                                                <  SRR4302543.5473023/94‑1 (MQ=60)
                GACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTG                                                                               <  SRR4302543.6100929/94‑1 (MQ=60)
                GACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTG                                                                               <  SRR4302543.6100930/94‑1 (MQ=60)
                GACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTG                                                                               <  SRR4302543.2979504/94‑1 (MQ=60)
                GACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTG                                                                               <  SRR4302543.2979503/94‑1 (MQ=60)
                 ACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGG                                                                              <  SRR4302543.6393494/94‑1 (MQ=60)
                 ACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGG                                                                              <  SRR4302543.7491856/94‑1 (MQ=60)
                  CTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGC                                                                             >  SRR4302543.3489525/1‑94 (MQ=60)
                  CTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGC                                                                             <  SRR4302543.2805564/94‑1 (MQ=60)
                  CTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGC                                                                             >  SRR4302543.568568/1‑94 (MQ=60)
                  CTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGC                                                                             >  SRR4302543.7240018/1‑94 (MQ=60)
                  CTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGC                                                                             >  SRR4302543.5128594/1‑94 (MQ=60)
                   TTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCC                                                                            <  SRR4302543.2243785/94‑1 (MQ=60)
                   TTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCC                                                                            <  SRR4302543.2586814/94‑1 (MQ=60)
                   TTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCC                                                                            >  SRR4302543.2551879/1‑94 (MQ=60)
                      CGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGA                                                                         >  SRR4302543.6784215/1‑94 (MQ=60)
                      CGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGA                                                                         >  SRR4302543.4887072/1‑94 (MQ=60)
                       GATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAA                                                                        >  SRR4302543.100334/1‑94 (MQ=60)
                       GATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAA                                                                        >  SRR4302543.714435/1‑94 (MQ=60)
                        ATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAAC                                                                       <  SRR4302543.1629406/94‑1 (MQ=60)
                        ATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAAC                                                                       <  SRR4302543.38071/94‑1 (MQ=60)
                        ATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAAC                                                                       >  SRR4302543.2631730/1‑94 (MQ=60)
                        ATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAAC                                                                       >  SRR4302543.7190508/1‑94 (MQ=60)
                         TGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACC                                                                      <  SRR4302543.1597622/94‑1 (MQ=60)
                         TGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACC                                                                      <  SRR4302543.3851409/94‑1 (MQ=60)
                         TGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACC                                                                      >  SRR4302543.4329225/1‑94 (MQ=60)
                         TGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACC                                                                      <  SRR4302543.7186708/94‑1 (MQ=60)
                          GTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCG                                                                     <  SRR4302543.514375/94‑1 (MQ=60)
                          GTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCG                                                                     >  SRR4302543.1888811/1‑94 (MQ=60)
                          GTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCG                                                                     <  SRR4302543.5024506/94‑1 (MQ=60)
                          GTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCG                                                                     >  SRR4302543.6878736/1‑94 (MQ=60)
                          GTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCG                                                                     <  SRR4302543.245171/94‑1 (MQ=60)
                          GTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCG                                                                     <  SRR4302543.3372215/94‑1 (MQ=60)
                           TTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGA                                                                    >  SRR4302543.5110523/1‑94 (MQ=60)
                               TCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGAC                                                                <  SRR4302543.6040515/94‑1 (MQ=60)
                                CGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACG                                                               >  SRR4302543.668123/1‑94 (MQ=60)
                                CGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACG                                                               <  SRR4302543.3136258/94‑1 (MQ=60)
                                CGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACG                                                               >  SRR4302543.6867593/1‑94 (MQ=60)
                                  CCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCG                                                             >  SRR4302543.4084612/1‑94 (MQ=60)
                                  CCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCC                                                             >  SRR4302543.5129061/1‑94 (MQ=60)
                                  CCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCAGGAACCGATGACGCG                                                             >  SRR4302543.2765280/1‑94 (MQ=60)
                                  CCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCAGCATCCA‑GGGGGCGCCGAAAGCATGGTGGCCGGAACCGATGACGCG                                                             <  SRR4302543.2011965/94‑1 (MQ=60)
                                   CTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGC                                                            >  SRR4302543.7271732/1‑94 (MQ=60)
                                   CTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGC                                                            >  SRR4302543.7203677/1‑94 (MQ=60)
                                   CTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGC                                                            >  SRR4302543.6139105/1‑94 (MQ=60)
                                    TATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCC                                                           >  SRR4302543.3985336/1‑94 (MQ=60)
                                    TATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCC                                                           >  SRR4302543.6917032/1‑94 (MQ=60)
                                     ATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCT                                                          >  SRR4302543.963757/1‑94 (MQ=60)
                                     ATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCT                                                          >  SRR4302543.2759592/1‑94 (MQ=60)
                                     ATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCT                                                          >  SRR4302543.7743892/1‑94 (MQ=60)
                                     ATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCT                                                          >  SRR4302543.7416372/1‑94 (MQ=60)
                                      TACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTG                                                         <  SRR4302543.4026850/94‑1 (MQ=60)
                                      TACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTG                                                         <  SRR4302543.739305/94‑1 (MQ=60)
                                        CCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAA                                                       >  SRR4302543.534062/1‑94 (MQ=60)
                                         CGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAAT                                                      >  SRR4302543.4925770/1‑94 (MQ=60)
                                         CGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAAT                                                      >  SRR4302543.7224104/1‑94 (MQ=60)
                                         CGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAAT                                                      <  SRR4302543.8007927/94‑1 (MQ=60)
                                         CGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAAT                                                      >  SRR4302543.1652130/1‑94 (MQ=60)
                                          GGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGGGCGCCGAAAGCATTGTGGCCGGAACCGATCACGCGCCTGAAT                                                      >  SRR4302543.3816592/1‑94 (MQ=60)
                                          GGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATG                                                     >  SRR4302543.5716590/1‑94 (MQ=60)
                                          GGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATG                                                     <  SRR4302543.5575073/94‑1 (MQ=60)
                                          GGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATG                                                     <  SRR4302543.6989610/94‑1 (MQ=60)
                                           GTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGC                                                    <  SRR4302543.5612209/94‑1 (MQ=60)
                                           GTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGC                                                    >  SRR4302543.5906961/1‑94 (MQ=60)
                                           GTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGC                                                    >  SRR4302543.5906962/1‑94 (MQ=60)
                                           GTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGC                                                    <  SRR4302543.3249263/94‑1 (MQ=60)
                                            TGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCG                                                   <  SRR4302543.7876588/94‑1 (MQ=60)
                                             GCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGA                                                  >  SRR4302543.911540/1‑94 (MQ=60)
                                             GCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGA                                                  <  SRR4302543.5971284/94‑1 (MQ=60)
                                              CTTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAT                                                 <  SRR4302543.2946501/94‑1 (MQ=60)
                                              CGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAT                                                 <  SRR4302543.3839565/94‑1 (MQ=60)
                                              CGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAT                                                 <  SRR4302543.975542/94‑1 (MQ=60)
                                              CGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAT                                                 <  SRR4302543.5840401/94‑1 (MQ=60)
                                              CGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGAG                                                 >  SRR4302543.2431969/1‑94 (MQ=60)
                                               GTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATG                                                <  SRR4302543.837576/94‑1 (MQ=60)
                                               GTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATG                                                <  SRR4302543.2007250/94‑1 (MQ=60)
                                               GTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATG                                                >  SRR4302543.4052249/1‑94 (MQ=60)
                                               GTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATG                                                >  SRR4302543.1431336/1‑94 (MQ=60)
                                                TTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGG                                               <  SRR4302543.6882174/94‑1 (MQ=60)
                                                 TCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGG                                              >  SRR4302543.7126360/1‑94 (MQ=60)
                                                 TCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGG                                              <  SRR4302543.498965/94‑1 (MQ=60)
                                                 TCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGG                                              >  SRR4302543.449560/1‑94 (MQ=60)
                                                  CTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGG                                             <  SRR4302543.933463/94‑1 (MQ=60)
                                                  CTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGG                                             >  SRR4302543.2706055/1‑94 (MQ=60)
                                                  CTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGG                                             >  SRR4302543.5921858/1‑94 (MQ=60)
                                                  CTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGG                                             >  SRR4302543.3725252/1‑94 (MQ=60)
                                                  CTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGG                                             >  SRR4302543.3583064/1‑94 (MQ=60)
                                                  CTTTACGCTTTGCTATTCACCGCTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGG                                             <  SRR4302543.5811909/94‑1 (MQ=60)
                                                   TTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGA                                            >  SRR4302543.1079898/1‑94 (MQ=60)
                                                   TTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGA                                            >  SRR4302543.4975469/1‑94 (MQ=60)
                                                    TTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGAT                                           <  SRR4302543.732597/94‑1 (MQ=60)
                                                    TTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGAT                                           <  SRR4302543.4249569/94‑1 (MQ=60)
                                                    TTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGAT                                           <  SRR4302543.131390/94‑1 (MQ=60)
                                                     TACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATG                                          >  SRR4302543.3983633/1‑94 (MQ=60)
                                                     TACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATG                                          <  SRR4302543.3882310/94‑1 (MQ=60)
                                                      TCGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGC                                         <  SRR4302543.5423733/94‑1 (MQ=60)
                                                      ACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGC                                         >  SRR4302543.6237290/1‑94 (MQ=60)
                                                      ACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGC                                         >  SRR4302543.481795/1‑94 (MQ=60)
                                                      ACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGC                                         <  SRR4302543.3871133/94‑1 (MQ=60)
                                                        GCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCG                                       >  SRR4302543.7734890/1‑94 (MQ=60)
                                                        GCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCG                                       >  SRR4302543.6291337/1‑94 (MQ=60)
                                                        GCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCG                                       >  SRR4302543.2976170/1‑94 (MQ=60)
                                                         CTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGT                                      >  SRR4302543.5489629/1‑94 (MQ=60)
                                                         CTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGT                                      <  SRR4302543.2552555/94‑1 (MQ=60)
                                                          TTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCggac                                     >  SRR4302543.824937/1‑90 (MQ=60)
                                                           TTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCT                                    <  SRR4302543.4364146/94‑1 (MQ=60)
                                                           TTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCT                                    <  SRR4302543.3147684/94‑1 (MQ=60)
                                                            TGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTA                                   <  SRR4302543.4159353/94‑1 (MQ=60)
                                                            TGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTA                                   >  SRR4302543.5839143/1‑94 (MQ=60)
                                                            TGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTA                                   <  SRR4302543.5680698/94‑1 (MQ=60)
                                                            TGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTA                                   >  SRR4302543.1336901/1‑94 (MQ=60)
                                                            TGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGAGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGCATGCGATGGGGATcgcgtcca                                   >  SRR4302543.5839144/1‑86 (MQ=60)
                                                             TCTATACACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTAT                                  <  SRR4302543.5733388/94‑1 (MQ=60)
                                                             GCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTAT                                  <  SRR4302543.2323660/94‑1 (MQ=60)
                                                             GCTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTAT                                  <  SRR4302543.7430781/94‑1 (MQ=60)
                                                              CTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATC                                 <  SRR4302543.6683171/94‑1 (MQ=60)
                                                              CTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATC                                 <  SRR4302543.6683172/94‑1 (MQ=60)
                                                              CTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATC                                 >  SRR4302543.5961191/1‑94 (MQ=60)
                                                              CTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATC                                 >  SRR4302543.2082036/1‑94 (MQ=60)
                                                              CTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATC                                 <  SRR4302543.4928447/94‑1 (MQ=60)
                                                              CTATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATC                                 <  SRR4302543.555256/94‑1 (MQ=60)
                                                               TATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCG                                <  SRR4302543.301477/94‑1 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               >  SRR4302543.2920192/1‑94 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               >  SRR4302543.1552849/1‑94 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               >  SRR4302543.2249876/1‑94 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               >  SRR4302543.6985406/1‑94 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               >  SRR4302543.3738577/1‑94 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               <  SRR4302543.7895508/94‑1 (MQ=60)
                                                                ATTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGC                               >  SRR4302543.566292/1‑94 (MQ=60)
                                                                 TTCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCC                              >  SRR4302543.5037098/1‑94 (MQ=60)
                                                                  TCACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCA                             <  SRR4302543.7851742/94‑1 (MQ=60)
                                                                   CACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAT                            <  SRR4302543.2159365/94‑1 (MQ=60)
                                                                    ACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATG                           >  SRR4302543.7731276/1‑94 (MQ=60)
                                                                    ACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATG                           >  SRR4302543.3824434/1‑94 (MQ=60)
                                                                    ACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATG                           >  SRR4302543.246509/1‑94 (MQ=60)
                                                                    ACCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATG                           >  SRR4302543.2446702/1‑94 (MQ=60)
                                                                     CCGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGT                          >  SRR4302543.2896977/1‑94 (MQ=60)
                                                                      CGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTG                         >  SRR4302543.2818166/1‑94 (MQ=60)
                                                                      CGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTG                         >  SRR4302543.2818165/1‑94 (MQ=60)
                                                                      CGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTG                         >  SRR4302543.6627556/1‑94 (MQ=60)
                                                                      CGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTG                         >  SRR4302543.467556/1‑94 (MQ=60)
                                                                      CGTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTG                         >  SRR4302543.7347119/1‑94 (MQ=60)
                                                                       GTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGCTGCCGTCTATCGCCATGTGG                        >  SRR4302543.561891/1‑94 (MQ=60)
                                                                       GTTGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGG                        <  SRR4302543.2598552/94‑1 (MQ=60)
                                                                         TGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGAT                      <  SRR4302543.2330983/94‑1 (MQ=60)
                                                                         TGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGAT                      <  SRR4302543.4675653/94‑1 (MQ=60)
                                                                         TGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGAT                      >  SRR4302543.5185719/1‑94 (MQ=60)
                                                                         TGAAAGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGAT                      <  SRR4302543.5951282/94‑1 (MQ=60)
                                                                             AGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGaga                  >  SRR4302543.582434/1‑91 (MQ=60)
                                                                             AGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAG                  >  SRR4302543.7004257/1‑94 (MQ=60)
                                                                             AGCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAG                  <  SRR4302543.615934/94‑1 (MQ=60)
                                                                              GCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGT                 <  SRR4302543.4431289/94‑1 (MQ=60)
                                                                              GCCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGT                 <  SRR4302543.3856411/94‑1 (MQ=60)
                                                                               CCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTG                <  SRR4302543.7915130/94‑1 (MQ=60)
                                                                               CCATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTG                >  SRR4302543.2220074/1‑94 (MQ=60)
                                                                               CCATCATCCA‑GGGGGCCCCGAAAGCATTGTCGCCGGAACCGATGACGCGCCTGAATGCGCAGGGGATGCCGTCTATCGCCATGTGGACGCAGTG                >  SRR4302543.2220073/1‑94 (MQ=60)
                                                                                CATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGC               <  SRR4302543.3969839/94‑1 (MQ=60)
                                                                                CATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGC               >  SRR4302543.758411/1‑94 (MQ=60)
                                                                                 ATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGTCATGTGGATGCAGTGCG              >  SRR4302543.6483597/1‑94 (MQ=60)
                                                                                 ATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCG              >  SRR4302543.1928126/1‑94 (MQ=60)
                                                                                 ATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCG              >  SRR4302543.778004/1‑94 (MQ=60)
                                                                                 ATCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCG              <  SRR4302543.5187994/94‑1 (MQ=60)
                                                                                  TCATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGG             <  SRR4302543.4564028/94‑1 (MQ=60)
                                                                                  TCATCCA‑GGGGGCGCCGAAACCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGG             <  SRR4302543.6713137/94‑1 (MQ=60)
                                                                                   CATCCA‑GGGGGCGCCGACAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCAGGTGGATGCAGTGCGGG            <  SRR4302543.4438236/94‑1 (MQ=60)
                                                                                   CATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGG            >  SRR4302543.1434791/1‑94 (MQ=60)
                                                                                    ATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGT           >  SRR4302543.4726029/1‑94 (MQ=60)
                                                                                    ATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGT           >  SRR4302543.6790503/1‑94 (MQ=60)
                                                                                    ATCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGT           >  SRR4302543.1645581/1‑94 (MQ=60)
                                                                                     TCCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTT          <  SRR4302543.3121052/94‑1 (MQ=60)
                                                                                      CCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTG         >  SRR4302543.24359/1‑94 (MQ=60)
                                                                                      CCA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTG         >  SRR4302543.1688588/1‑94 (MQ=60)
                                                                                       CA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGG        >  SRR4302543.7281877/1‑94 (MQ=60)
                                                                                       CA‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGG        <  SRR4302543.7257969/94‑1 (MQ=60)
                                                                                        A‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGCTGCAGTGCGGGTTGGT       >  SRR4302543.3138379/1‑94 (MQ=60)
                                                                                        A‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGT       <  SRR4302543.168989/94‑1 (MQ=60)
                                                                                        A‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGT       >  SRR4302543.2864911/1‑94 (MQ=60)
                                                                                        A‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGT       <  SRR4302543.19566/94‑1 (MQ=60)
                                                                                        A‑GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGT       >  SRR4302543.2736348/1‑94 (MQ=60)
                                                                                          GGGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTT      >  SRR4302543.2469592/1‑94 (MQ=60)
                                                                                           GGGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTA     >  SRR4302543.1737967/1‑94 (MQ=60)
                                                                                            GGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTAC    <  SRR4302543.6384684/94‑1 (MQ=60)
                                                                                            GGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTAC    <  SRR4302543.6384685/94‑1 (MQ=60)
                                                                                            GGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTAC    <  SRR4302543.1036587/94‑1 (MQ=60)
                                                                                            GGGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGGTGGTTAC    >  SRR4302543.6557540/1‑94 (MQ=60)
                                                                                             GGCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACT   >  SRR4302543.3112738/1‑94 (MQ=60)
                                                                                              GCGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTG  >  SRR4302543.4456614/1‑94 (MQ=60)
                                                                                              |                                                                                             
TTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCA‑GGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTG  >  NC_000913/4382350‑4382536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑