Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,392,958 G→A G439S (GGC→AGC)  yjeF → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,392,9580GA99.5% 1330.0 / ‑6.1 364G439S (GGC→AGC) yjeFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (175/187);  total (175/189)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 390 total aligned reads displayed.

GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG  >  NC_000913/4392868‑4393047
                                                                                           |                                                                                         
gCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCggcagc                                                                                         >  2:5915233/1‑93 (MQ=255)
 cGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGgcagca                                                                                        <  2:1695740/93‑1 (MQ=255)
 cGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGgcagca                                                                                        >  2:4017898/1‑93 (MQ=255)
  ggTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCAt                                                                                       <  1:6221166/93‑1 (MQ=255)
   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                      >  1:632281/1‑93 (MQ=255)
   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                      >  2:6288725/1‑93 (MQ=255)
   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                      >  2:1600962/1‑93 (MQ=255)
   gTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATg                                                                                      <  2:1937977/93‑1 (MQ=255)
    tGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATgg                                                                                     >  2:5017756/1‑93 (MQ=255)
     gCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATggg                                                                                    <  1:7374950/93‑1 (MQ=255)
     gCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATggg                                                                                    <  2:1132358/93‑1 (MQ=255)
       tGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCg                                                                                  >  1:7111311/1‑93 (MQ=255)
        gAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGTATGGCGAGCGGCAGCATGGGCGa                                                                                 <  2:3316036/93‑1 (MQ=255)
        gAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGa                                                                                 >  1:5955744/1‑93 (MQ=255)
        gAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGa                                                                                 <  1:3764812/93‑1 (MQ=255)
        gAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGa                                                                                 <  2:264277/93‑1 (MQ=255)
         aaaGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAt                                                                                <  2:482740/93‑1 (MQ=255)
         aaaGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAt                                                                                <  1:4772336/93‑1 (MQ=255)
            ggTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAtgtg                                                                             >  2:6400354/1‑93 (MQ=255)
              tGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGct                                                                           <  2:4128331/93‑1 (MQ=255)
               gCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTATGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctc                                                                          <  1:4086358/93‑1 (MQ=255)
               gCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctc                                                                          <  1:489496/93‑1 (MQ=255)
               gCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctc                                                                          <  1:2853733/93‑1 (MQ=255)
                   gAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTg                                                                      <  2:6856290/93‑1 (MQ=255)
                    aaCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTgg                                                                     <  2:6626912/93‑1 (MQ=255)
                    aaCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTgg                                                                     <  2:7224797/93‑1 (MQ=255)
                     aCCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGt                                                                    <  2:212153/93‑1 (MQ=255)
                       cGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtat                                                                  >  2:3605406/1‑93 (MQ=255)
                       cGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtat                                                                  >  1:7447494/1‑93 (MQ=255)
                          ggTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtattat                                                               <  2:291752/93‑1 (MQ=255)
                          ggTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGtattat                                                               <  2:812670/93‑1 (MQ=255)
                           gTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTattatt                                                              >  1:1190636/1‑93 (MQ=255)
                           gTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTattatt                                                              <  2:5249098/93‑1 (MQ=255)
                            tCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTg                                                             <  2:7500575/93‑1 (MQ=255)
                            tCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTg                                                             <  1:2087974/93‑1 (MQ=255)
                            tCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTg                                                             >  2:3847582/1‑93 (MQ=255)
                            gcgccgcCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTg                                                             <  1:676879/92‑1 (MQ=255)
                             cgccgcCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTgg                                                            >  1:862083/1‑93 (MQ=255)
                               ccgccCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgcg                                                          <  1:2623276/93‑1 (MQ=255)
                                cgccCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGTATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGgcgc                                                         <  2:6551573/93‑1 (MQ=255)
                                 gccCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCa                                                        <  2:5171439/93‑1 (MQ=255)
                                  cccATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAt                                                       <  1:3602343/93‑1 (MQ=255)
                                  cccATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAt                                                       >  1:6999974/1‑93 (MQ=255)
                                     aTCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGc                                                    <  1:1807854/93‑1 (MQ=255)
                                     aTCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGc                                                    <  2:7657703/93‑1 (MQ=255)
                                     aTCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGc                                                    >  1:954447/1‑93 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                   >  1:1504160/1‑93 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                   >  1:4306160/1‑93 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCt                                                   >  1:2451625/1‑93 (MQ=255)
                                      tCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTCGCGCATTGCt                                                   >  1:2230170/1‑93 (MQ=255)
                                        cagACGCTTTAGGCCTCATTGA‑GCAGCGAAATGCAGGCATGGCGAGCGGCAGCATGGCCGATATGCCCTCTGGTATTATAGGCGCATTGCTTg                                                 <  2:2766298/91‑1 (MQ=255)
                                        cTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTg                                                 <  2:6105679/93‑1 (MQ=255)
                                         tGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTgg                                                >  1:3946893/1‑93 (MQ=255)
                                         tGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTgg                                                <  1:6889446/93‑1 (MQ=255)
                                          gACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTggg                                               >  1:3699851/1‑93 (MQ=255)
                                           aCGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGc                                              >  2:4704259/1‑93 (MQ=255)
                                            cGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCa                                             >  1:339212/1‑93 (MQ=255)
                                             gCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaa                                            >  1:3210201/1‑93 (MQ=255)
                                             gCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaa                                            >  2:5706380/1‑93 (MQ=255)
                                              cTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaa                                           >  1:2138119/1‑93 (MQ=255)
                                               tttAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaa                                          >  2:7121652/1‑93 (MQ=255)
                                               tctAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaa                                          >  2:6392125/3‑93 (MQ=255)
                                                ttAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaaa                                         >  1:720686/1‑93 (MQ=255)
                                                ttAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCaaaaa                                         >  1:3570514/1‑93 (MQ=255)
                                                 tAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAc                                        <  2:196892/93‑1 (MQ=255)
                                                 tAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAc                                        <  1:6667320/93‑1 (MQ=255)
                                                  aGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACt                                       >  2:4468417/1‑93 (MQ=255)
                                                  aGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACt                                       <  1:277808/93‑1 (MQ=255)
                                                     cATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGctctc                                                                        <  2:3723112/57‑1 (MQ=255)
                                                        tattGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGcc                                 >  2:1250013/1‑93 (MQ=255)
                                                        tattGATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGcc                                 >  1:1997865/1‑93 (MQ=255)
                                                            gATGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTat                             <  2:2487845/93‑1 (MQ=255)
                                                             aTGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatg                            <  2:230792/93‑1 (MQ=255)
                                                              tGCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatga                           <  2:6227934/93‑1 (MQ=255)
                                                               gCCG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTatgat                          >  2:1869815/1‑93 (MQ=255)
                                                                 cG‑GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGc                        >  1:1658666/1‑93 (MQ=255)
                                                                    gAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAg                      >  2:3050485/1‑93 (MQ=255)
                                                                        tGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtg                  <  1:5125027/93‑1 (MQ=255)
                                                                        tGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCtg                  >  1:7293005/1‑93 (MQ=255)
                                                                           aGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGc               >  1:3667290/1‑93 (MQ=255)
                                                                            ggCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCa              >  1:4593140/1‑93 (MQ=255)
                                                                            ggCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCa              >  1:3284342/1‑93 (MQ=255)
                                                                             gCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg             >  1:7656393/1‑93 (MQ=255)
                                                                             gCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg             >  1:3159086/1‑93 (MQ=255)
                                                                             gCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAg             <  1:7785253/93‑1 (MQ=255)
                                                                              cATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAgg            >  2:4332267/1‑93 (MQ=255)
                                                                              cATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAgg            <  1:2536593/93‑1 (MQ=255)
                                                                               aTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc           <  1:443769/93‑1 (MQ=255)
                                                                               aTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGc           >  2:3437495/1‑93 (MQ=255)
                                                                                tGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCt          >  1:1297098/1‑93 (MQ=255)
                                                                                  gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt        <  1:1001241/93‑1 (MQ=255)
                                                                                  gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt        <  1:5259194/93‑1 (MQ=255)
                                                                                  gCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgt        <  1:4937488/93‑1 (MQ=255)
                                                                                   cGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgtg       <  2:1330519/93‑1 (MQ=255)
                                                                                   cGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCtgtg       <  1:545750/93‑1 (MQ=255)
                                                                                    gAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTgtgt      <  1:5797844/93‑1 (MQ=255)
                                                                                      gcggcaGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg    <  2:4254244/93‑1 (MQ=255)
                                                                                      gcggcaGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcg    >  1:6008665/1‑93 (MQ=255)
                                                                                       cggcagCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgc   <  1:5616583/93‑1 (MQ=255)
                                                                                        ggcagcATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTcgcg  >  2:5616240/1‑93 (MQ=255)
                                                                                           |                                                                                         
GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCG‑GAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG  >  NC_000913/4392868‑4393047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 222 total aligned reads displayed.
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  NC_000913/4392865‑4393051
                                                                                             |                                                                                             
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               >  SRR4302543.2275387/1‑94 (MQ=60)
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCA                                                                                               <  SRR4302543.4295547/94‑1 (MQ=60)
 TAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAG                                                                                              <  SRR4302543.3729750/94‑1 (MQ=60)
 TAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAG                                                                                              >  SRR4302543.5631599/1‑94 (MQ=60)
 TAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAG                                                                                              <  SRR4302543.1618785/94‑1 (MQ=60)
 TAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAG                                                                                              >  SRR4302543.339354/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302543.2547725/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             >  SRR4302543.793744/1‑94 (MQ=60)
  AGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGC                                                                                             <  SRR4302543.3465388/94‑1 (MQ=60)
   GCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCA                                                                                            <  SRR4302543.3536857/94‑1 (MQ=60)
   GCGGTCCTGAAAGGTGCCGCAACCGGGGTCGCCGCCCATCCTGACGCTTTAGTCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCA                                                                                            <  SRR4302543.5674100/94‑1 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          >  SRR4302543.638057/1‑94 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATG                                                                                          <  SRR4302543.6279894/94‑1 (MQ=60)
     GGTGCTGAAAGGTGCCGGAACCGTGGACGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGAGAGCGGCAGCATG                                                                                          >  SRR4302543.4064683/1‑94 (MQ=60)
       ggcgGAAAGTTGCCGGAACCGTGGTCGCCGCCCAGCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302543.3214698/90‑1 (MQ=60)
       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302543.4008698/94‑1 (MQ=60)
       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302543.3214699/94‑1 (MQ=60)
       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302543.5908721/94‑1 (MQ=60)
       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302543.3586015/94‑1 (MQ=60)
       TGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGG                                                                                        <  SRR4302543.4965414/94‑1 (MQ=60)
        GCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGC                                                                                       <  SRR4302543.5645033/94‑1 (MQ=60)
        GCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGC                                                                                       <  SRR4302543.7445845/94‑1 (MQ=60)
         GTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      <  SRR4302543.6888381/94‑1 (MQ=60)
         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      <  SRR4302543.6235957/94‑1 (MQ=60)
         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302543.7180394/1‑94 (MQ=60)
         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302543.388023/1‑94 (MQ=60)
         CTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCG                                                                                      >  SRR4302543.3870560/1‑94 (MQ=60)
          TGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGA                                                                                     >  SRR4302543.6010257/1‑94 (MQ=60)
           GAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAT                                                                                    <  SRR4302543.3797951/94‑1 (MQ=60)
           GAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAT                                                                                    <  SRR4302543.3621824/94‑1 (MQ=60)
           GAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGAT                                                                                    <  SRR4302543.1236407/94‑1 (MQ=60)
            AAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   <  SRR4302543.3661778/94‑1 (MQ=60)
            AAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   <  SRR4302543.4815948/94‑1 (MQ=60)
            AAAGGTGCCGGAACCGCGCTGGCCGCCCATCCTGACCCTTTAGGCATGATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATG                                                                                   <  SRR4302543.3055479/94‑1 (MQ=60)
             AAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGT                                                                                  <  SRR4302543.1775133/94‑1 (MQ=60)
              AGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTG                                                                                 <  SRR4302543.7141267/94‑1 (MQ=60)
              AGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTG                                                                                 >  SRR4302543.1991981/1‑94 (MQ=60)
              AGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTG                                                                                 <  SRR4302543.1653066/94‑1 (MQ=60)
               GGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGC                                                                                >  SRR4302543.7849525/1‑94 (MQ=60)
                GTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCT                                                                               <  SRR4302543.6302/94‑1 (MQ=60)
                 TGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTC                                                                              >  SRR4302543.2131467/1‑94 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTATGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302543.4122997/94‑1 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302543.2578350/94‑1 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302543.2879771/94‑1 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302543.728758/94‑1 (MQ=60)
                  GCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCT                                                                             <  SRR4302543.494016/94‑1 (MQ=60)
                   CCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTC                                                                            >  SRR4302543.1572883/1‑94 (MQ=60)
                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           <  SRR4302543.5776569/94‑1 (MQ=60)
                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           >  SRR4302543.2754535/1‑94 (MQ=60)
                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           >  SRR4302543.5172305/1‑94 (MQ=60)
                    CGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCT                                                                           >  SRR4302543.7038968/1‑94 (MQ=60)
                     GGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTG                                                                          <  SRR4302543.361684/94‑1 (MQ=60)
                       AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGT                                                                        >  SRR4302543.1370990/1‑94 (MQ=60)
                       AACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAACGGCAGCATGGGCGATGTGCTCTCTGGT                                                                        >  SRR4302543.903975/1‑94 (MQ=60)
                        ACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTA                                                                       >  SRR4302543.1255624/1‑94 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      <  SRR4302543.7348899/94‑1 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      >  SRR4302543.7518860/1‑94 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      >  SRR4302543.4085/1‑94 (MQ=60)
                         CCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTAT                                                                      >  SRR4302543.2602889/1‑94 (MQ=60)
                          CGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATT                                                                     >  SRR4302543.7472304/1‑94 (MQ=60)
                          CGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATT                                                                     >  SRR4302543.2401383/1‑94 (MQ=60)
                           GTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTA                                                                    >  SRR4302543.2127742/1‑94 (MQ=60)
                            TGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTAT                                                                   >  SRR4302543.4389425/1‑94 (MQ=60)
                             GGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATT                                                                  <  SRR4302543.2105726/94‑1 (MQ=60)
                             GGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATT                                                                  >  SRR4302543.1201375/1‑94 (MQ=60)
                             GGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATT                                                                  <  SRR4302543.7426566/94‑1 (MQ=60)
                             GGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATT                                                                  <  SRR4302543.2489541/94‑1 (MQ=60)
                              GTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTG                                                                 >  SRR4302543.5951141/1‑94 (MQ=60)
                              GTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTG                                                                 <  SRR4302543.1365150/94‑1 (MQ=60)
                              GTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGCGCGATGTGCTCTCTGGTATTATTG                                                                 >  SRR4302543.4611334/1‑94 (MQ=60)
                               TCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                <  SRR4302543.2278183/94‑1 (MQ=60)
                               TCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                <  SRR4302543.2106628/94‑1 (MQ=60)
                               TCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                <  SRR4302543.3252127/94‑1 (MQ=60)
                               TCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                >  SRR4302543.869767/1‑94 (MQ=60)
                               GCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGG                                                                <  SRR4302543.683109/94‑1 (MQ=60)
                                 GCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCG                                                              <  SRR4302543.7406291/94‑1 (MQ=60)
                                  CCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGC                                                             <  SRR4302543.2647031/94‑1 (MQ=60)
                                  CCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGC                                                             <  SRR4302543.3323539/94‑1 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           <  SRR4302543.7275985/94‑1 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           >  SRR4302543.7066246/1‑94 (MQ=60)
                                    GCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCAT                                                           >  SRR4302543.8003132/1‑94 (MQ=60)
                                     CCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATT                                                          >  SRR4302543.4356719/1‑94 (MQ=60)
                                     CCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATT                                                          <  SRR4302543.3634132/94‑1 (MQ=60)
                                      CCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTG                                                         >  SRR4302543.5052874/1‑94 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302543.4350982/94‑1 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302543.2900924/94‑1 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       >  SRR4302543.1517676/1‑94 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       <  SRR4302543.6015462/94‑1 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCT                                                       >  SRR4302543.2473742/1‑94 (MQ=60)
                                        ATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTCGCGCATTGCT                                                       >  SRR4302543.2250316/1‑94 (MQ=60)
                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     <  SRR4302543.7284649/94‑1 (MQ=60)
                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     <  SRR4302543.3580105/94‑1 (MQ=60)
                                          CCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTG                                                     <  SRR4302543.3239341/94‑1 (MQ=60)
                                           CTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGG                                                    <  SRR4302543.2198002/94‑1 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGCTGTGCTCTCTGGTATTATTGGCGCATTGCTTGGC                                                   >  SRR4302543.208736/1‑94 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   >  SRR4302543.3732378/1‑94 (MQ=60)
                                            TGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGG                                                   <  SRR4302543.2162726/94‑1 (MQ=60)
                                             GACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGC                                                  >  SRR4302543.2088592/1‑94 (MQ=60)
                                              ACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCA                                                 >  SRR4302543.342329/1‑94 (MQ=60)
                                              ACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGCATTATTGGCGCATTGCTTGGGCA                                                 >  SRR4302543.4932559/1‑94 (MQ=60)
                                              ACGCTTAAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGGGCTCACTGGTATTATTGGCGCATTGCCTGGGCA                                                 >  SRR4302543.7114017/1‑94 (MQ=60)
                                               CGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAA                                                <  SRR4302543.6889507/94‑1 (MQ=60)
                                               CGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAA                                                >  SRR4302543.7133421/1‑94 (MQ=60)
                                               CGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAA                                                >  SRR4302543.3239180/1‑94 (MQ=60)
                                                GCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAA                                               >  SRR4302543.2157257/1‑94 (MQ=60)
                                                GCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAA                                               >  SRR4302543.6139209/1‑94 (MQ=60)
                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302543.1211252/1‑94 (MQ=60)
                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302543.2677525/1‑94 (MQ=60)
                                                 CTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAA                                              >  SRR4302543.2819242/1‑94 (MQ=60)
                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302543.727192/1‑94 (MQ=60)
                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302543.3602081/1‑94 (MQ=60)
                                                  TTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAA                                             >  SRR4302543.7391603/1‑94 (MQ=60)
                                                   TTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAC                                            <  SRR4302543.6017958/94‑1 (MQ=60)
                                                   TTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAAC                                            >  SRR4302543.544144/1‑94 (MQ=60)
                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302543.6730264/94‑1 (MQ=60)
                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302543.7256185/94‑1 (MQ=60)
                                                    TAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACT                                           <  SRR4302543.8003814/94‑1 (MQ=60)
                                                     AGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTG                                          <  SRR4302543.280263/94‑1 (MQ=60)
                                                     AGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTG                                          <  SRR4302543.1191122/94‑1 (MQ=60)
                                                      GGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGT                                         >  SRR4302543.675839/1‑94 (MQ=60)
                                                       GCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTagagtggagatcggaagagcggttcagcaggaatg                                        >  SRR4302543.3755854/1‑59 (MQ=60)
                                                       GCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTTGTATTATTGGCGCATTGCTTGGGCAAAAACTGTC                                        >  SRR4302543.7630881/1‑94 (MQ=60)
                                                       GCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTC                                        <  SRR4302543.916521/94‑1 (MQ=60)
                                                       GCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTC                                        <  SRR4302543.5639892/94‑1 (MQ=60)
                                                        CATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGAATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCG                                       <  SRR4302543.7001512/94‑1 (MQ=60)
                                                        CATTAATGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCG                                       <  SRR4302543.3974004/94‑1 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302543.3792650/1‑94 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302543.6753240/1‑94 (MQ=60)
                                                         ATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGC                                      >  SRR4302543.3342448/1‑94 (MQ=60)
                                                          TTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCC                                     >  SRR4302543.2015795/1‑94 (MQ=60)
                                                          TTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCC                                     >  SRR4302543.2051237/1‑94 (MQ=60)
                                                            ATTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGT                                   <  SRR4302543.3273527/94‑1 (MQ=60)
                                                             TTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTA                                  <  SRR4302543.5297353/94‑1 (MQ=60)
                                                             TTGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTA                                  >  SRR4302543.6985005/1‑94 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 <  SRR4302543.5446206/94‑1 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 <  SRR4302543.7714033/94‑1 (MQ=60)
                                                              TGATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTAT                                 <  SRR4302543.1587963/94‑1 (MQ=60)
                                                               GATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATG                                >  SRR4302543.7501207/1‑94 (MQ=60)
                                                                ATGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGA                               >  SRR4302543.6778116/1‑94 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              >  SRR4302543.3634168/1‑94 (MQ=60)
                                                                 TGCCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGAATTGCTTGGGCAAAAACTGTCGCCGTATGAT                              <  SRR4302543.489662/94‑1 (MQ=60)
                                                                   CCGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGC                            >  SRR4302543.1673516/1‑94 (MQ=60)
                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           >  SRR4302543.4282050/1‑94 (MQ=60)
                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           >  SRR4302543.5129006/1‑94 (MQ=60)
                                                                    CGGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCA                           <  SRR4302543.6353392/94‑1 (MQ=60)
                                                                     GGGAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAG                          <  SRR4302543.3708163/94‑1 (MQ=60)
                                                                     GGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAG                          >  SRR4302543.186704/1‑94 (MQ=60)
                                                                     GGAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAG                          <  SRR4302543.3708341/94‑1 (MQ=60)
                                                                      GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAATCTGTCGCCGTATGATGCAGC                         <  SRR4302543.2235974/94‑1 (MQ=60)
                                                                      GAAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGAAAAAACTGTCGCCGTATGATGCAGC                         <  SRR4302543.1793344/94‑1 (MQ=60)
                                                                       AAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCC                        >  SRR4302543.3194523/1‑94 (MQ=60)
                                                                       AAATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCC                        >  SRR4302543.1983901/1‑94 (MQ=60)
                                                                        AATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCT                       <  SRR4302543.4194105/94‑1 (MQ=60)
                                                                        AATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCT                       <  SRR4302543.6582105/94‑1 (MQ=60)
                                                                         ATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTG                      >  SRR4302543.7363388/1‑94 (MQ=60)
                                                                         ATGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTG                      <  SRR4302543.2603962/94‑1 (MQ=60)
                                                                          TGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT                     >  SRR4302543.4548333/1‑94 (MQ=60)
                                                                          TGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT                     <  SRR4302543.4831285/94‑1 (MQ=60)
                                                                          TGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT                     >  SRR4302543.2810213/1‑94 (MQ=60)
                                                                          TGCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGT                     <  SRR4302543.5171363/94‑1 (MQ=60)
                                                                           GCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTG                    <  SRR4302543.4184908/94‑1 (MQ=60)
                                                                           GCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTG                    <  SRR4302543.5969254/94‑1 (MQ=60)
                                                                           GCAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTG                    >  SRR4302543.1169175/1‑94 (MQ=60)
                                                                            CAGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGC                   >  SRR4302543.3699493/1‑94 (MQ=60)
                                                                             AGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCA                  <  SRR4302543.2863618/94‑1 (MQ=60)
                                                                             AGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCA                  >  SRR4302543.4774195/1‑94 (MQ=60)
                                                                             AGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCA                  >  SRR4302543.3313986/1‑94 (MQ=60)
                                                                             AGGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCA                  >  SRR4302543.4634855/1‑94 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGactgtcgag                 >  SRR4302543.884352/1‑85 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302543.7728976/1‑94 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302543.3187526/1‑94 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 <  SRR4302543.5200992/94‑1 (MQ=60)
                                                                              GGCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAG                 >  SRR4302543.5608551/1‑94 (MQ=60)
                                                                               GCATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGG                <  SRR4302543.7858650/94‑1 (MQ=60)
                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               >  SRR4302543.6580670/1‑94 (MQ=60)
                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               <  SRR4302543.2559702/94‑1 (MQ=60)
                                                                                CATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGC               >  SRR4302543.5676891/1‑94 (MQ=60)
                                                                                 CTGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              <  SRR4302543.447922/94‑1 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302543.3741599/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302543.1308631/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              >  SRR4302543.497969/1‑94 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              <  SRR4302543.447921/94‑1 (MQ=60)
                                                                                 ATGGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCT              <  SRR4302543.631732/94‑1 (MQ=60)
                                                                                   GGCGAGCGGCAGCATGGGCGATGTGCTCTCTGGGATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCggg            >  SRR4302543.6055499/1‑91 (MQ=60)
                                                                                    GCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTG           <  SRR4302543.1010086/94‑1 (MQ=60)
                                                                                    GCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTG           <  SRR4302543.5306624/94‑1 (MQ=60)
                                                                                    GCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTG           <  SRR4302543.4982450/94‑1 (MQ=60)
                                                                                    GCGAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTG           <  SRR4302543.5306625/94‑1 (MQ=60)
                                                                                      GAGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTC         >  SRR4302543.6856434/1‑94 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        >  SRR4302543.7479833/1‑94 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        <  SRR4302543.7723942/94‑1 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        >  SRR4302543.181320/1‑94 (MQ=60)
                                                                                       AGCGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCG        >  SRR4302543.6064023/1‑94 (MQ=60)
                                                                                         CGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG      <  SRR4302543.4735519/94‑1 (MQ=60)
                                                                                         CGGCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCG      <  SRR4302543.5667765/94‑1 (MQ=60)
                                                                                           GCAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCA    <  SRR4302543.4048133/94‑1 (MQ=60)
                                                                                            CAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCAC   <  SRR4302543.3640171/94‑1 (MQ=60)
                                                                                            CAGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCAC   >  SRR4302543.6892198/1‑94 (MQ=60)
                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  SRR4302543.5958561/1‑94 (MQ=60)
                                                                                             AGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  SRR4302543.5698470/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTAGCGGTGCTGAAAGGTGCCGGAACCGTGGTCGCCGCCCATCCTGACGCTTTAGGCATTATTGATGCCGGAAATGCAGGCATGGCGAGCGGCGGCATGGGCGATGTGCTCTCTGGTATTATTGGCGCATTGCTTGGGCAAAAACTGTCGCCGTATGATGCAGCCTGTGCAGGCTGTGTCGCGCACG  >  NC_000913/4392865‑4393051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑