Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,474,826 +A intergenic (+1/+44) yjgL → / ← argI hypothetical protein/ornithine carbamoyltransferase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,474,8231.A100.0% 1001.7 / NA 273*617K (TAA→AAA) yjgLhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (157/116);  total (157/116)

Only 100 of 302 total aligned reads displayed.

TTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTAT  >  NC_000913/4474731‑4474910
                                                                                             |                                                                                       
ttAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat                                                                                          <  2:6901324/93‑1 (MQ=255)
    tGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaa                                                                                     >  1:3238102/1‑92 (MQ=255)
    tGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaa                                                                                     >  1:7466325/1‑92 (MQ=255)
     gAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaat                                                                                    >  2:3670222/1‑91 (MQ=255)
      aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaata                                                                                   >  2:6133382/1‑90 (MQ=255)
      aaCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaata                                                                                   <  1:1919424/93‑4 (MQ=255)
       aCAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCat‑aaaataa                                                                                  <  1:2934822/93‑5 (MQ=255)
         aGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg                                                                                 <  1:5875953/93‑1 (MQ=255)
          gCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt                                                                                >  2:1055698/1‑93 (MQ=255)
          gCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgt                                                                                <  2:5558217/93‑1 (MQ=255)
           cGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg                                                                               <  1:4469940/93‑1 (MQ=255)
            gTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGa                                                                              >  1:534105/1‑93 (MQ=255)
            gTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGa                                                                              <  2:3240586/93‑1 (MQ=255)
              ttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                                            >  1:6458112/1‑93 (MQ=255)
              ttAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATg                                                                            >  1:5485125/1‑93 (MQ=255)
                aaataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc                                                                          >  1:3295763/1‑93 (MQ=255)
                 aataaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCg                                                                         >  1:4959935/1‑93 (MQ=255)
                    aaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa                                                                      <  2:6235675/93‑1 (MQ=255)
                    aaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa                                                                      >  2:1934937/1‑93 (MQ=255)
                    aaaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa                                                                      >  2:4888115/1‑93 (MQ=255)
                     aaCTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAt                                                                     >  1:6837509/1‑93 (MQ=255)
                       cTAAATCCACCGGTAATTTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACTTATCTCTGTATAAAATAAAGTGATGCCGGGATgg                                                                   >  2:7017408/1‑93 (MQ=255)
                       cTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATgg                                                                   <  2:2410567/93‑1 (MQ=255)
                       cTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATgg                                                                   >  2:800934/1‑93 (MQ=255)
                         aaaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGtt                                                                 <  1:416199/93‑1 (MQ=255)
                         aaaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGtt                                                                 >  2:6694918/1‑93 (MQ=255)
                          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                >  1:221513/1‑93 (MQ=255)
                          aaTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGttt                                                                >  1:1074953/1‑93 (MQ=255)
                           aTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTg                                                               >  1:3881120‑M1/1‑92 (MQ=255)
                           aTCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTg                                                               >  1:5263481‑M1/1‑92 (MQ=255)
                            tCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGAGGAAGAATAGAAATATCACTGCATAAAATAATATGATGCCGGGATGGTTTgt                                                              >  1:3931058‑M1/1‑91 (MQ=255)
                             ccACCGGAAAATTTACGTATAGCAATAGACAACTTTGGGTGGAAGAATAGACCAATCACTGCATAAAATAATTTGATGCCGGGATGGttttgt                                                             >  1:1740789/1‑90 (MQ=255)
                              cACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                            >  1:4686567‑M1/1‑89 (MQ=255)
                              cACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                            <  2:3107580‑M1/93‑5 (MQ=255)
                                ccGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                          >  1:3174177‑M1/1‑87 (MQ=255)
                                ccGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                          >  1:2511122‑M1/1‑87 (MQ=255)
                                  ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                        >  2:5645266‑M1/1‑85 (MQ=255)
                                  ggAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                        <  1:1770566‑M1/93‑9 (MQ=255)
                                   gAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                       >  2:6743870‑M1/1‑84 (MQ=255)
                                   gAAAATTTAAGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                       <  2:2277661‑M1/93‑10 (MQ=255)
                                        tttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                  >  1:2729112‑M1/1‑79 (MQ=255)
                                        tttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                  >  1:2016979‑M1/1‑79 (MQ=255)
                                         ttACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                                                 >  2:2733004‑M1/1‑78 (MQ=255)
                                          tACGTATAGCAATAGAACACTTTGGGTGGAAGAATCGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatct                                                >  1:3450523‑M1/1‑77 (MQ=255)
                                          tACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatct                                                <  2:3817243‑M1/93‑17 (MQ=255)
                                           aCGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                               <  2:6558638‑M1/93‑18 (MQ=255)
                                           aCGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                               >  1:3524320‑M1/1‑76 (MQ=255)
                                            cGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttt                                              >  1:2112103‑M1/1‑75 (MQ=255)
                                             gTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                             <  2:7204578‑M1/93‑20 (MQ=255)
                                               ataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                           <  1:7794371‑M1/93‑22 (MQ=255)
                                               ataGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                           <  2:159351‑M1/93‑22 (MQ=255)
                                                taGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                          <  2:7695371‑M1/93‑23 (MQ=255)
                                                  gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                        >  2:5827737‑M1/1‑69 (MQ=255)
                                                  gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                        >  2:1186239‑M1/1‑69 (MQ=255)
                                                  gCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                        <  1:4270361‑M1/93‑25 (MQ=255)
                                                    aaTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                      >  2:745760‑M1/1‑67 (MQ=255)
                                                     aTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                     <  1:6723281‑M1/93‑28 (MQ=255)
                                                     aTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                     <  2:7588091‑M1/93‑28 (MQ=255)
                                                        gAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagca                                  >  2:3755741‑M1/1‑63 (MQ=255)
                                                          acacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaat                                <  2:4629978‑M1/93‑33 (MQ=255)
                                                           cacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                               <  2:685526‑M1/93‑34 (MQ=255)
                                                           cacTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                               >  2:2517539‑M1/1‑60 (MQ=255)
                                                             cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                             >  1:2749731‑M1/1‑58 (MQ=255)
                                                             cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                             <  1:611383‑M1/93‑36 (MQ=255)
                                                             cTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                             <  2:5899024‑M1/93‑36 (MQ=255)
                                                              tttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                            <  2:5055108‑M1/93‑37 (MQ=255)
                                                              tttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                            <  1:6917466‑M1/93‑37 (MQ=255)
                                                               ttGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattt                           <  1:7632835‑M1/93‑38 (MQ=255)
                                                                 gggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttac                         <  2:6403983‑M1/93‑40 (MQ=255)
                                                                 gggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttac                         <  2:3308021‑M1/93‑40 (MQ=255)
                                                                 gggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttac                         <  1:3757808‑M1/93‑40 (MQ=255)
                                                                  ggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                        <  2:2800044‑M1/93‑41 (MQ=255)
                                                                  ggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                        <  1:1604930‑M1/93‑41 (MQ=255)
                                                                  ggTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                        >  1:7852622‑M1/1‑53 (MQ=255)
                                                                   gTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                       >  2:7379942‑M1/1‑52 (MQ=255)
                                                                      gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagt                    >  2:4664829‑M1/1‑49 (MQ=255)
                                                                      gaagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagt                    <  2:5503268‑M1/93‑45 (MQ=255)
                                                                       aagaaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtg                   >  1:69481‑M1/1‑48 (MQ=255)
                                                                          aaTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcg                >  1:5623220‑M1/1‑45 (MQ=255)
                                                                           aTAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgc               >  2:101717‑M1/1‑44 (MQ=255)
                                                                             aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcga             >  2:6277243‑M1/1‑42 (MQ=255)
                                                                             aGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcga             <  2:182100‑M1/93‑52 (MQ=255)
                                                                              gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgac            <  1:844464‑M1/93‑53 (MQ=255)
                                                                              gACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgac            <  1:3090962‑M1/93‑53 (MQ=255)
                                                                               aCCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgacc           <  2:4737437‑M1/93‑54 (MQ=255)
                                                                                ccTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgacaa          >  1:6378023‑M1/1‑39 (MQ=255)
                                                                                 cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccat         <  2:2968006‑M1/93‑56 (MQ=255)
                                                                                 cTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccat         <  2:1325057‑M1/93‑56 (MQ=255)
                                                                                  tATCACTGCATAAAATAATGTGGTGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagcgtcgcgaccata        >  2:4038822‑M1/1‑37 (MQ=255)
                                                                                  tATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatc        >  1:1413457‑M1/1‑37 (MQ=255)
                                                                                   aTCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatca       >  2:713449‑M1/1‑36 (MQ=255)
                                                                                    tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcac      <  2:394136‑M1/93‑59 (MQ=255)
                                                                                    tCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcac      <  1:7312307‑M1/93‑59 (MQ=255)
                                                                                     cACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcacc     <  1:1196380‑M1/93‑60 (MQ=255)
                                                                                     cACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcacc     <  2:3429288‑M1/93‑60 (MQ=255)
                                                                                        tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct  >  1:4528440‑M1/1‑31 (MQ=255)
                                                                                        tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct  <  1:4845978‑M1/93‑63 (MQ=255)
                                                                                        tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct  >  2:4387091‑M1/1‑31 (MQ=255)
                                                                                        tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct  >  2:4300630‑M1/1‑31 (MQ=255)
                                                                                        tGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgaccatcaccgct  >  2:1730409‑M1/1‑31 (MQ=255)
                                                                                             |                                                                                       
TTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTTCCGCATCTTATAGCGATCAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTAT  >  NC_000913/4474731‑4474910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑CCGCATC‑TTATAGCGAT‑‑CAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  NC_000913/4474733‑4474909
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AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAA                                                                                                <  SRR4302543.1781813/94‑1 (MQ=60)
AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAA                                                                                                >  SRR4302543.5602748/1‑94 (MQ=60)
 ATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAA                                                                                               >  SRR4302543.3267392/1‑94 (MQ=60)
 ATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAA                                                                                               >  SRR4302543.7590912/1‑94 (MQ=60)
 ATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAA                                                                                               >  SRR4302543.7537839/1‑94 (MQ=60)
  TGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAat                                                                                              <  SRR4302543.1096353/94‑3 (MQ=60)
   GAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAAata                                                                                             >  SRR4302543.4394432/1‑91 (MQ=60)
    AACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAA                                                                                             >  SRR4302543.4742154/1‑94 (MQ=60)
    AACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAA                                                                                             <  SRR4302543.1936728/94‑1 (MQ=60)
    AACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAA                                                                                             >  SRR4302543.1269439/1‑94 (MQ=60)
     ACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAT                                                                                            <  SRR4302543.2961466/94‑1 (MQ=60)
     ACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAT                                                                                            <  SRR4302543.7877605/94‑1 (MQ=60)
      CAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATTACTGCATAAAATAATG                                                                                           >  SRR4302543.2603395/1‑94 (MQ=60)
      CAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATG                                                                                           <  SRR4302543.2895332/94‑1 (MQ=60)
      CAGCGTTTAAATAAACTAAAGCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATG                                                                                           <  SRR4302543.6145144/94‑1 (MQ=60)
       AGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGT                                                                                          <  SRR4302543.5929486/94‑1 (MQ=60)
        GCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTG                                                                                         <  SRR4302543.6418140/94‑1 (MQ=60)
        GCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTG                                                                                         >  SRR4302543.7096014/1‑94 (MQ=60)
         CGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGA                                                                                        >  SRR4302543.539050/1‑94 (MQ=60)
         CGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGA                                                                                        <  SRR4302543.4510474/94‑1 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302543.2889943/1‑94 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302543.4676614/1‑94 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       <  SRR4302543.3074393/94‑1 (MQ=60)
          GTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGAT                                                                                       >  SRR4302543.4312643/1‑94 (MQ=60)
           TTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATG                                                                                      >  SRR4302543.6518965/1‑94 (MQ=60)
           TTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATG                                                                                      >  SRR4302543.5575413/1‑94 (MQ=60)
           TTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATG                                                                                      >  SRR4302543.5534848/1‑94 (MQ=60)
            TTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGC                                                                                     >  SRR4302543.583132/1‑94 (MQ=60)
             TAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCC                                                                                    >  SRR4302543.3325497/1‑94 (MQ=60)
              AAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCG                                                                                   >  SRR4302543.5095734/1‑94 (MQ=60)
              AAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCG                                                                                   >  SRR4302543.5005082/1‑94 (MQ=60)
              AAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCG                                                                                   >  SRR4302543.182418/1‑94 (MQ=60)
              AAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATAACTGAATAAAATAATGTGATGCCG                                                                                   >  SRR4302543.2358644/1‑94 (MQ=60)
               AATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGCGTGGCAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGG                                                                                  >  SRR4302543.7012551/1‑94 (MQ=60)
                  AAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAT                                                                               <  SRR4302543.7918123/94‑1 (MQ=60)
                  AAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAT                                                                               <  SRR4302543.1452605/94‑1 (MQ=60)
                  AAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGAT                                                                               >  SRR4302543.6902049/1‑94 (MQ=60)
                   AACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATG                                                                              >  SRR4302543.3268633/1‑94 (MQ=60)
                     CTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGT                                                                            <  SRR4302543.1731837/94‑1 (MQ=60)
                     CTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGT                                                                            >  SRR4302543.7533020/1‑94 (MQ=60)
                     CTAAATACACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGT                                                                            >  SRR4302543.3934740/1‑94 (MQ=60)
                      TAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTT                                                                           >  SRR4302543.3883519/1‑94 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGTATAAAATAATGTGATGCCGGGATGGTTT                                                                          <  SRR4302543.3494715/94‑1 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          >  SRR4302543.6438477/1‑94 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          >  SRR4302543.1084614/1‑94 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          <  SRR4302543.5970900/94‑1 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          <  SRR4302543.420088/94‑1 (MQ=60)
                       AAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT                                                                          >  SRR4302543.223419/1‑94 (MQ=60)
                        AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑G                                                                 >  SRR4302543.5310966/1‑94 (MQ=60)
                        AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑G                                                                 >  SRR4302543.3915383/1‑94 (MQ=60)
                        AATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑G                                                                 >  SRR4302543.3601646/1‑94 (MQ=60)
                         ATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgt                                                                        >  SRR4302543.4825107/1‑92 (MQ=60)
                         ATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgt                                                                        >  SRR4302543.4532634/1‑92 (MQ=60)
                         ATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgt                                                                        <  SRR4302543.1153789/94‑3 (MQ=60)
                         ATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGAGGAAGAATAGAAATATCACTGCATAAAATAATATGATGCCGGGATGGTTTgt                                                                        >  SRR4302543.3965771/1‑92 (MQ=60)
                          TCCACCGGAAAATTTACGTATAGCAATAGACAACTTTGGGTGGAAGAATAGACCAATCACTGCATAAAATAATTTGATGCCGGGATGGTTTtgt                                                                       >  SRR4302543.1756319/1‑91 (MQ=60)
                          TCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                                       <  SRR4302543.5358676/94‑4 (MQ=60)
                          TCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgta                                                                       >  SRR4302543.7928653/1‑91 (MQ=60)
                           CCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                                      >  SRR4302543.4729328/1‑90 (MQ=60)
                           CCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                                      >  SRR4302543.3137342/1‑90 (MQ=60)
                           CCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtat                                                                      >  SRR4302543.5874121/1‑90 (MQ=60)
                            CACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatt                                                                     >  SRR4302543.2055445/1‑89 (MQ=60)
                             ACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                                    >  SRR4302543.3202775/1‑88 (MQ=60)
                             ACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                                    >  SRR4302543.1322241/1‑88 (MQ=60)
                             ACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtattt                                                                    >  SRR4302543.2533920/1‑88 (MQ=60)
                              CCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttc                                                                   >  SRR4302543.6728661/1‑87 (MQ=60)
                               CGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcc                                                                  <  SRR4302543.7012046/94‑9 (MQ=60)
                                GGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                                 <  SRR4302543.4240270/94‑10 (MQ=60)
                                GGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                                 <  SRR4302543.1786396/94‑10 (MQ=60)
                                GGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttccc                                                                 <  SRR4302543.4240269/94‑10 (MQ=60)
                                 GAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccg                                                                <  SRR4302543.4938877/94‑11 (MQ=60)
                                 GAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccg                                                                <  SRR4302543.7558936/94‑11 (MQ=60)
                                   AAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggc                                                              <  SRR4302543.1899703/94‑13 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            >  SRR4302543.1768231/1‑80 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            >  SRR4302543.2035104/1‑80 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            <  SRR4302543.7389258/94‑15 (MQ=60)
                                     ATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcat                                                            >  SRR4302543.2753946/1‑80 (MQ=60)
                                      TTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatc                                                           <  SRR4302543.7254668/94‑16 (MQ=60)
                                       TTACGTATAGCAATAGAACACTTTGGGTGGAAGAATCGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatct                                                          >  SRR4302543.3481424/1‑78 (MQ=60)
                                       TTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatct                                                          <  SRR4302543.5039974/94‑17 (MQ=60)
                                        TACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                         >  SRR4302543.3555747/1‑77 (MQ=60)
                                        TACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctt                                                         >  SRR4302543.341493/1‑77 (MQ=60)
                                         ACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttt                                                        >  SRR4302543.2131007/1‑76 (MQ=60)
                                          CGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatcttta                                                       >  SRR4302543.3920743/1‑75 (MQ=60)
                                           GTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttat                                                      >  SRR4302543.6603503/1‑74 (MQ=60)
                                            TATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                                     <  SRR4302543.2140877/94‑22 (MQ=60)
                                            TATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttata                                                     >  SRR4302543.5505019/1‑73 (MQ=60)
                                             ATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                                    >  SRR4302543.3358971/1‑72 (MQ=60)
                                             ATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                                    <  SRR4302543.7867824/94‑23 (MQ=60)
                                             ATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatag                                                    >  SRR4302543.2175020/1‑72 (MQ=60)
                                               AGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGgttgtatttcccggcatctttatagca                                                  >  SRR4302543.3992410/1‑67 (MQ=60)
                                               AGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcg                                                  >  SRR4302543.3992408/1‑70 (MQ=60)
                                                GCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcga                                                 <  SRR4302543.4309166/94‑26 (MQ=60)
                                                 CAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgat                                                >  SRR4302543.1477961/1‑68 (MQ=60)
                                                  AATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                               <  SRR4302543.3217181/94‑28 (MQ=60)
                                                  AATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgata                                               >  SRR4302543.5520121/1‑67 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              <  SRR4302543.2768843/94‑29 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              >  SRR4302543.2008629/1‑66 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              >  SRR4302543.6926858/1‑66 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              <  SRR4302543.6794404/94‑29 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              <  SRR4302543.6794403/94‑29 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              <  SRR4302543.6786815/94‑29 (MQ=60)
                                                   ATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatag                                              >  SRR4302543.3200809/1‑66 (MQ=60)
                                                        ACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaatt                                         >  SRR4302543.202835/1‑61 (MQ=60)
                                                          ACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                                       >  SRR4302543.2063856/1‑59 (MQ=60)
                                                          ACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattat                                       >  SRR4302543.2774740/1‑59 (MQ=60)
                                                           CTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                                      >  SRR4302543.2455599/1‑58 (MQ=60)
                                                           CTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                                      <  SRR4302543.4904211/94‑37 (MQ=60)
                                                           CTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatt                                      <  SRR4302543.616968/94‑37 (MQ=60)
                                                            TTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattt                                     <  SRR4302543.6982499/94‑38 (MQ=60)
                                                             TTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattta                                    >  SRR4302543.6238981/1‑56 (MQ=60)
                                                             TTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattattta                                    <  SRR4302543.7705257/94‑39 (MQ=60)
                                                               GGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                                  >  SRR4302543.7926611/1‑54 (MQ=60)
                                                               GGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttact                                  <  SRR4302543.3790875/94‑41 (MQ=60)
                                                                GGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactg                                 <  SRR4302543.1619253/94‑42 (MQ=60)
                                                                    GAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtg                             >  SRR4302543.70115/1‑49 (MQ=60)
                                                                     AAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgt                            <  SRR4302543.6939042/94‑47 (MQ=60)
                                                                      AGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtc                           >  SRR4302543.861240/1‑47 (MQ=60)
                                                                      AGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtc                           >  SRR4302543.6474369/1‑47 (MQ=60)
                                                                      AGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtc                           >  SRR4302543.4540640/1‑47 (MQ=60)
                                                                      AGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtc                           <  SRR4302543.2703628/94‑48 (MQ=60)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcg                          <  SRR4302543.6085615/94‑49 (MQ=60)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcg                          <  SRR4302543.2946255/94‑49 (MQ=60)
                                                                       GAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcg                          >  SRR4302543.5674466/1‑46 (MQ=60)
                                                                         ATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcg                        >  SRR4302543.3270920/1‑44 (MQ=60)
                                                                         ATAGACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcg                        <  SRR4302543.4625194/94‑51 (MQ=60)
                                                                            GACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACC                    <  SRR4302543.3118758/94‑1 (MQ=21)
                                                                            GACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACC                    <  SRR4302543.851944/94‑1 (MQ=21)
                                                                             ACCTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTgtatttcccggcatctttatagcgatagcaattatttactgagtgtcgcgacaa                    >  SRR4302543.6438029/1‑40 (MQ=60)
                                                                               CTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATC                 >  SRR4302543.3266033/1‑94 (MQ=16)
                                                                               CTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATC                 <  SRR4302543.2757877/94‑1 (MQ=16)
                                                                               CTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATC                 >  SRR4302543.7776263/1‑94 (MQ=16)
                                                                               CTATCACTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATC                 >  SRR4302543.1426244/1‑94 (MQ=16)
                                                                                     CTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCT           >  SRR4302543.4569656/1‑94 (MQ=16)
                                                                                     CTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCT           >  SRR4302543.3645443/1‑94 (MQ=16)
                                                                                     CTGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCT           >  SRR4302543.7979933/1‑94 (MQ=16)
                                                                                      TGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTT          >  SRR4302543.3541283/1‑94 (MQ=18)
                                                                                      TGCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTT          <  SRR4302543.4890165/94‑1 (MQ=18)
                                                                                       GCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTT         <  SRR4302543.5431335/94‑1 (MQ=19)
                                                                                       GCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTT         >  SRR4302543.6958130/1‑94 (MQ=19)
                                                                                       GCATAAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTT         >  SRR4302543.4443342/1‑94 (MQ=19)
                                                                                         ataAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGA       >  SRR4302543.972912/4‑94 (MQ=23)
                                                                                          taAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGAT      <  SRR4302543.4620595/92‑1 (MQ=25)
                                                                                          taAAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGAT      >  SRR4302543.861384/3‑94 (MQ=25)
                                                                                          A‑AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATA     >  SRR4302543.1439426/1‑94 (MQ=26)
                                                                                          A‑AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATA     >  SRR4302543.602067/1‑94 (MQ=26)
                                                                                            AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAG    <  SRR4302543.7533058/94‑1 (MQ=28)
                                                                                            AAATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAG    >  SRR4302543.7877603/1‑94 (MQ=28)
                                                                                             AATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAGT   >  SRR4302543.608000/1‑94 (MQ=28)
                                                                                             AATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAGT   >  SRR4302543.4774461/1‑94 (MQ=28)
                                                                                             AATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAGT   <  SRR4302543.2810209/94‑1 (MQ=28)
                                                                                             AATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAGT   >  SRR4302543.3001623/1‑94 (MQ=28)
                                                                                              ATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAGTA  >  SRR4302543.5813870/1‑94 (MQ=28)
                                                                                              ATAATGTGATGCCGGGATGGTTTGTATTTCCCGGCATCTTTATAGCGATAGCA‑ATTATTTACTGAGTGTCGCGACCATCACCGCTTTGATAGTA  <  SRR4302543.5723611/94‑1 (MQ=28)
                                                                                              |                                                                                              
AATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCAT‑AAATAATGTGATGCCGGGATGGTTT‑‑‑‑‑‑‑‑CCGCATC‑TTATAGCGAT‑‑CAGATTATTTACTGAGCGTCGCGACCATCACCGCTTTGATAGTA  >  NC_000913/4474733‑4474909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑