Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,041 Δ1 bp intergenic (‑151/‑269) b4256 ← / → yjgN hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,0410G.99.6% 1082.2 / ‑3.7 238intergenic (‑151/‑269)b4256/yjgNhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base . (71/166);  total (72/166)
Fisher's exact test for biased strand distribution p-value = 3.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT  >  NC_000913/4476953‑4477127
                                                                                        |                                                                                       
 gTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAtt                                                                                   >  2:4677771/1‑93 (MQ=255)
  tCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTa                                                                                  <  2:574828/93‑1 (MQ=255)
  tCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTa                                                                                  >  1:3102205/1‑93 (MQ=255)
   cAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTAt                                                                                 <  1:976822/93‑1 (MQ=255)
     aGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATcc                                                                               >  2:4350899/1‑93 (MQ=255)
     aGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATcc                                                                               <  1:1830481/93‑1 (MQ=255)
     aGATTCGGATCGGCGACGGTGTAGCCCTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATcc                                                                               <  1:4500097/93‑1 (MQ=255)
      gATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCt                                                                              >  1:5902035/1‑93 (MQ=255)
      gATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCt                                                                              <  2:7484314/93‑1 (MQ=255)
      gATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCt                                                                              >  1:3972574/1‑93 (MQ=255)
           ggATCGGCGACGGTGTAGCCTTTATCAGCGGTACGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGc                                                                         <  1:7045006/93‑1 (MQ=255)
            gATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCg                                                                        >  2:5866899/1‑93 (MQ=255)
             aTCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGt                                                                       >  1:3437516/1‑93 (MQ=255)
             aTCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGt                                                                       <  1:2621581/93‑1 (MQ=255)
              tCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTc                                                                      <  2:3152339/93‑1 (MQ=255)
               cgtcGACGGTGTAGCTTTTTTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCa                                                                     <  1:6657038/90‑1 (MQ=255)
               cGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCa                                                                     <  2:7404822/93‑1 (MQ=255)
                 gCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTGTCCTGCAGCGTCAgg                                                                   <  1:1594353/93‑1 (MQ=255)
                  cGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc                                                                  >  2:7158990/1‑93 (MQ=255)
                  cGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc                                                                  <  1:1527823/93‑1 (MQ=255)
                  cGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGc                                                                  <  1:6492418/93‑1 (MQ=255)
                   gACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcg                                                                 >  1:5689932/1‑93 (MQ=255)
                   gACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcg                                                                 >  1:7397728/1‑93 (MQ=255)
                    gcGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGAAGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcga                                                                <  2:6398602/92‑1 (MQ=255)
                    aCGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcga                                                                <  2:35157/93‑1 (MQ=255)
                    aCGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcga                                                                <  1:3009394/93‑1 (MQ=255)
                    aCGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcga                                                                <  2:6332023/93‑1 (MQ=255)
                     cGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgac                                                               <  1:7908304/93‑1 (MQ=255)
                      ggTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacg                                                              <  2:5214354/93‑1 (MQ=255)
                       gtgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgc                                                             >  1:616282/1‑92 (MQ=255)
                         gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                           >  1:6772596/1‑90 (MQ=255)
                         gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                           <  2:1349133/93‑4 (MQ=255)
                          tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcatt                                                          >  1:4619124/1‑89 (MQ=255)
                          tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcatt                                                          <  1:7258806/93‑5 (MQ=255)
                            gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAc                                                        <  1:4270534/93‑1 (MQ=255)
                             ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                       <  2:7725589/93‑1 (MQ=255)
                             ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                       <  2:927268/93‑1 (MQ=255)
                             ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                       <  1:5718136/93‑1 (MQ=255)
                              ccttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                      <  1:2155507/91‑1 (MQ=255)
                              cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                      <  2:7320024/93‑1 (MQ=255)
                               tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAgg                                                     <  1:5068548/93‑1 (MQ=255)
                               tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAgg                                                     >  1:601428/1‑93 (MQ=255)
                                ttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGt                                                    <  1:4411724/93‑1 (MQ=255)
                                 tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTg                                                   <  2:7795473/93‑1 (MQ=255)
                                 tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTg                                                   >  2:7126274/1‑93 (MQ=255)
                                  aTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGa                                                  <  2:2848352/93‑1 (MQ=255)
                                   tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                 >  2:5984694/1‑93 (MQ=255)
                                    cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAtt                                                >  1:7120537/1‑93 (MQ=255)
                                      gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                              <  1:1648125/93‑1 (MQ=255)
                                       cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcg                                             <  1:2529528/93‑1 (MQ=255)
                                          tAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggcg                                          >  1:1012734/1‑93 (MQ=255)
                                           aaGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGgcgc                                         >  2:4368118/1‑93 (MQ=255)
                                             gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCaa                                       <  2:2571182/93‑1 (MQ=255)
                                               ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                     >  1:7383801/1‑93 (MQ=255)
                                               ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                     >  2:2693145/1‑93 (MQ=255)
                                               ccGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATg                                     <  1:2381841/93‑1 (MQ=255)
                                                cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGt                                    >  2:5482923/1‑93 (MQ=255)
                                                  tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                  <  2:3317639/93‑1 (MQ=255)
                                                  tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                  >  2:7868620/1‑93 (MQ=255)
                                                   aTTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAt                                 <  1:1096036/93‑1 (MQ=255)
                                                   aTTCGGCGGATACCTGACGAATGACGCGGGCGATAG‑GAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCACTGttct                                 >  1:4873429/1‑91 (MQ=255)
                                                    ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAtt                                >  2:5972140/1‑93 (MQ=255)
                                                    ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAtt                                <  2:5359063/93‑1 (MQ=255)
                                                       ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                             >  2:6961092/1‑93 (MQ=255)
                                                       ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                             <  1:1173465/93‑1 (MQ=255)
                                                       ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                             <  2:610186/93‑1 (MQ=255)
                                                       ggcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCAt                             <  2:4853627/93‑1 (MQ=255)
                                                        gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                            <  2:5320833/93‑1 (MQ=255)
                                                        gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                            <  2:2364603/93‑1 (MQ=255)
                                                        gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                            <  2:7551164/93‑1 (MQ=255)
                                                         cggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAg                           <  2:2091887/93‑1 (MQ=255)
                                                          ggATACCTGACGAATGACGCGGGCGATAGC‑AGTATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                          <  2:384635/93‑1 (MQ=255)
                                                             tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                       >  2:3963158/1‑93 (MQ=255)
                                                             tACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTa                       <  2:887616/93‑1 (MQ=255)
                                                              aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa                      <  1:3815448/93‑1 (MQ=255)
                                                               ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc                     <  1:4237150/93‑1 (MQ=255)
                                                               ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc                     >  1:2305378/1‑93 (MQ=255)
                                                                 tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                   >  2:5577889/1‑93 (MQ=255)
                                                                 tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                   <  1:2192722/93‑1 (MQ=255)
                                                                  gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                  <  1:7561007/93‑1 (MQ=255)
                                                                    cGAATGACGCGGGTGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                <  2:2471786/93‑1 (MQ=255)
                                                                    cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                <  2:3428756/93‑1 (MQ=255)
                                                                    cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                <  1:3031262/93‑1 (MQ=255)
                                                                      aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa              <  2:1437231/93‑1 (MQ=255)
                                                                      aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa              >  2:4570652/1‑93 (MQ=255)
                                                                       aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaa             <  2:1820930/93‑1 (MQ=255)
                                                                        tGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaa            <  1:800690/93‑1 (MQ=255)
                                                                         gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATTTTATTCATAGTTTAACCCaataaaaa           <  1:7216643/93‑1 (MQ=255)
                                                                             cgcgCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa       <  2:501007/90‑1 (MQ=255)
                                                                             cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCcataaaaataaa       <  1:7784568/93‑1 (MQ=255)
                                                                              gggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAg      <  2:4919167/93‑1 (MQ=255)
                                                                               ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAga     >  1:5334190/1‑92 (MQ=255)
                                                                               ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt     <  2:5891430/93‑1 (MQ=255)
                                                                               ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt     <  1:2774847/93‑1 (MQ=255)
                                                                                gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa    <  2:5705402/93‑1 (MQ=255)
                                                                                gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa    <  2:2576695/93‑1 (MQ=255)
                                                                                gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCCGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa    <  2:4514498/93‑1 (MQ=255)
                                                                                 cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTaa   <  2:4247072/93‑1 (MQ=255)
                                                                                 cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTaa   >  1:286007/1‑93 (MQ=255)
                                                                                  gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt  <  1:1050761/93‑1 (MQ=255)
                                                                                        |                                                                                       
CGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT  >  NC_000913/4476953‑4477127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA  >  NC_000913/4476948‑4477131
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CAGCTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCA                                                                                             >  SRR4302543.3880416/1‑94 (MQ=60)
CAGCTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCA                                                                                             >  SRR4302543.7695132/1‑94 (MQ=60)
  GCTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGG                                                                                          >  SRR4302543.4745530/1‑94 (MQ=60)
   CTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGA                                                                                         >  SRR4302543.1368583/1‑94 (MQ=60)
   CTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGA                                                                                         >  SRR4302543.6679638/1‑94 (MQ=60)
    TCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAT                                                                                        <  SRR4302543.3827477/94‑1 (MQ=60)
    TCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAT                                                                                        <  SRR4302543.6860602/94‑1 (MQ=60)
    TCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAT                                                                                        <  SRR4302543.7284600/94‑1 (MQ=60)
    TCGTCAAGATTCGGAGCGGCGACGGTGTAGCCATTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAT                                                                                        <  SRR4302543.4745551/94‑1 (MQ=60)
    CCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGGCAGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGAT                                                                                        <  SRR4302543.4745552/94‑1 (MQ=60)
     CGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATT                                                                                       <  SRR4302543.676969/94‑1 (MQ=60)
      GTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTA                                                                                      >  SRR4302543.3130103/1‑94 (MQ=60)
      GTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTA                                                                                      >  SRR4302543.3130102/1‑94 (MQ=60)
        CAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATC                                                                                    <  SRR4302543.985439/94‑1 (MQ=60)
        CAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATC                                                                                    >  SRR4302543.5821468/1‑94 (MQ=60)
         AAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCC                                                                                   <  SRR4302543.3549420/94‑1 (MQ=60)
         AAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATAACGCGGGCGATAGC‑AGGATTATCC                                                                                   <  SRR4302543.7895726/94‑1 (MQ=60)
          AGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCT                                                                                  >  SRR4302543.4007788/1‑94 (MQ=60)
          AGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCT                                                                                  <  SRR4302543.1846926/94‑1 (MQ=60)
          AGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCT                                                                                  >  SRR4302543.5955884/1‑94 (MQ=60)
          AGATTCGGATCGGCGACGGTGTAGCCCTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCT                                                                                  <  SRR4302543.4541005/94‑1 (MQ=60)
           GATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTG                                                                                 <  SRR4302543.5849826/94‑1 (MQ=60)
            ATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGC                                                                                <  SRR4302543.2203434/94‑1 (MQ=60)
              TCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAG                                                                              <  SRR4302543.3035257/94‑1 (MQ=60)
              TCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAG                                                                              <  SRR4302543.2104570/94‑1 (MQ=60)
               CGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGC                                                                             <  SRR4302543.827569/94‑1 (MQ=60)
                GGATCGGCGACGGTGTAGCCTTTATCAGCGGTACGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCG                                                                            <  SRR4302543.7113197/94‑1 (MQ=60)
                GGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCG                                                                            <  SRR4302543.6103599/94‑1 (MQ=60)
                 GATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGT                                                                           >  SRR4302543.3468284/1‑94 (MQ=60)
                  ATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTC                                                                          <  SRR4302543.8007421/94‑1 (MQ=60)
                  ATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTC                                                                          <  SRR4302543.2645328/94‑1 (MQ=60)
                  ATCGGCGACGGTGTAGCCCTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTC                                                                          >  SRR4302543.162224/1‑94 (MQ=60)
                    CGTCGACGGTGTAGCTTTTTTCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAG                                                                        <  SRR4302543.6719892/94‑1 (MQ=60)
                    CGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAG                                                                        <  SRR4302543.3948205/94‑1 (MQ=60)
                     GGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGG                                                                       <  SRR4302543.6601960/94‑1 (MQ=60)
                     GGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGG                                                                       <  SRR4302543.1119520/94‑1 (MQ=60)
                     GGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGG                                                                       <  SRR4302543.1119513/94‑1 (MQ=60)
                     GGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGG                                                                       <  SRR4302543.2305933/94‑1 (MQ=60)
                      GCGACGGTGTAGCCTTTATCAGCGGTAAGGCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGC                                                                      >  SRR4302543.1081578/1‑94 (MQ=60)
                      GCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTGTCCTGCAGCGTCAGGC                                                                      <  SRR4302543.1608579/94‑1 (MQ=60)
                       CGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCG                                                                     >  SRR4302543.7468765/1‑94 (MQ=60)
                       CGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCG                                                                     >  SRR4302543.5741860/1‑94 (MQ=60)
                       CGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCG                                                                     <  SRR4302543.6553581/94‑1 (MQ=60)
                       CGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCG                                                                     <  SRR4302543.1541533/94‑1 (MQ=60)
                         ACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC                                                                   <  SRR4302543.3036650/94‑1 (MQ=60)
                         ACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC                                                                   <  SRR4302543.2802513/94‑1 (MQ=60)
                         ACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGAC                                                                   <  SRR4302543.4553936/94‑1 (MQ=60)
                          CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG                                                                  <  SRR4302543.7982611/94‑1 (MQ=60)
                          CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG                                                                  >  SRR4302543.7521162/1‑94 (MQ=60)
                           GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG‑C                                                                >  SRR4302543.621919/1‑94 (MQ=60)
                           GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG‑C                                                                >  SRR4302543.762369/1‑94 (MQ=60)
                            GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGca                                                                >  SRR4302543.3070293/1‑92 (MQ=60)
                             TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGcat                                                               >  SRR4302543.276084/1‑91 (MQ=60)
                             TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGcat                                                               >  SRR4302543.6836572/1‑91 (MQ=60)
                              GTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATT                                                              >  SRR4302543.4661090/1‑94 (MQ=60)
                               TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTA                                                             >  SRR4302543.6697402/1‑94 (MQ=60)
                               TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTA                                                             <  SRR4302543.7328962/94‑1 (MQ=60)
                                AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                            <  SRR4302543.460862/94‑1 (MQ=60)
                                AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                            <  SRR4302543.1701112/94‑1 (MQ=60)
                                 GCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACA                                                           <  SRR4302543.4309339/94‑1 (MQ=60)
                                 GCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACA                                                           <  SRR4302543.2838690/94‑1 (MQ=60)
                                  CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                          <  SRR4302543.5770310/94‑1 (MQ=60)
                                  CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                          <  SRR4302543.764624/94‑1 (MQ=60)
                                   CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                         <  SRR4302543.1593754/94‑1 (MQ=60)
                                   CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                         >  SRR4302543.606933/1‑94 (MQ=60)
                                   CCTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                         <  SRR4302543.2174814/94‑1 (MQ=60)
                                    TTTATCCGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                        <  SRR4302543.5760395/94‑1 (MQ=60)
                                    TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                        <  SRR4302543.5114557/94‑1 (MQ=60)
                                    TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                        <  SRR4302543.6539861/94‑1 (MQ=60)
                                     TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTG                                                       <  SRR4302543.4451708/94‑1 (MQ=60)
                                      TATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGA                                                      <  SRR4302543.2382767/94‑1 (MQ=60)
                                       ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTGCAGGTGAT                                                     >  SRR4302543.4156836/1‑94 (MQ=60)
                                       ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAT                                                     <  SRR4302543.169320/94‑1 (MQ=60)
                                        TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                    <  SRR4302543.7311175/94‑1 (MQ=60)
                                        TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                    <  SRR4302543.7240420/94‑1 (MQ=60)
                                        TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                    >  SRR4302543.7189711/1‑94 (MQ=60)
                                           GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                 <  SRR4302543.734275/94‑1 (MQ=60)
                                           GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                 <  SRR4302543.1662865/94‑1 (MQ=60)
                                            CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGG                                                <  SRR4302543.2552544/94‑1 (MQ=60)
                                             GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                               >  SRR4302543.2583439/1‑94 (MQ=60)
                                             GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                               >  SRR4302543.4449316/1‑94 (MQ=60)
                                              GTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCG                                              >  SRR4302543.1021693/1‑94 (MQ=60)
                                                AAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCA                                            >  SRR4302543.5971908/1‑94 (MQ=60)
                                                 AGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAA                                           >  SRR4302543.7271014/1‑94 (MQ=60)
                                                  GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAT                                          <  SRR4302543.1909453/94‑1 (MQ=60)
                                                  GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAT                                          >  SRR4302543.1575133/1‑94 (MQ=60)
                                                   ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                         >  SRR4302543.1492780/1‑94 (MQ=60)
                                                   ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                         >  SRR4302543.7454758/1‑94 (MQ=60)
                                                   ACCGTATGCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                         <  SRR4302543.3040252/94‑1 (MQ=60)
                                                    CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                        <  SRR4302543.2403407/94‑1 (MQ=60)
                                                      GTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTA                                      <  SRR4302543.4452745/94‑1 (MQ=60)
                                                      GTATTCGCCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCATCGTCAGGCCACGCATTACAGGTGATTGCGGCGCAATGTTA                                      <  SRR4302543.4452939/94‑1 (MQ=60)
                                                       TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                     <  SRR4302543.2255921/94‑1 (MQ=60)
                                                       TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                     <  SRR4302543.5716632/94‑1 (MQ=60)
                                                       TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                     <  SRR4302543.7139136/94‑1 (MQ=60)
                                                       TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGCGAGTGCGGCGCGATGTTAT                                     >  SRR4302543.4917853/1‑94 (MQ=60)
                                                       TATTCGGCGGATACCTGACGAATGACGCGGGCGATAG‑GAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCACTGTTCT                                     >  SRR4302543.4917852/1‑94 (MQ=60)
                                                        ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATT                                    <  SRR4302543.1105900/94‑1 (MQ=60)
                                                         TTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTC                                   <  SRR4302543.746255/94‑1 (MQ=60)
                                                          TCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCA                                  <  SRR4302543.3350967/94‑1 (MQ=60)
                                                            GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                <  SRR4302543.1184102/94‑1 (MQ=60)
                                                             GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                               <  SRR4302543.1032399/94‑1 (MQ=60)
                                                              CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                              >  SRR4302543.6766687/1‑94 (MQ=60)
                                                                   ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                         >  SRR4302543.2326379/1‑94 (MQ=60)
                                                                   ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                         <  SRR4302543.7993805/94‑1 (MQ=60)
                                                                   ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                         <  SRR4302543.3849083/94‑1 (MQ=60)
                                                                    CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                        <  SRR4302543.4275588/94‑1 (MQ=60)
                                                                    CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                        <  SRR4302543.4275591/94‑1 (MQ=60)
                                                                      TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                      <  SRR4302543.2212418/94‑1 (MQ=60)
                                                                       GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                     <  SRR4302543.7632979/94‑1 (MQ=60)
                                                                       GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                     >  SRR4302543.7372310/1‑94 (MQ=60)
                                                                        ACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAAT                    <  SRR4302543.4226628/94‑1 (MQ=60)
                                                                         CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                   <  SRR4302543.3058635/94‑1 (MQ=60)
                                                                           AATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAA                 <  SRR4302543.5443453/94‑1 (MQ=60)
                                                                             TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA               <  SRR4302543.807733/94‑1 (MQ=60)
                                                                             TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA               <  SRR4302543.6926504/94‑1 (MQ=60)
                                                                              GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATTTTATTCATAGTTTAACCCAATAAAAAT              <  SRR4302543.7286590/94‑1 (MQ=60)
                                                                                 GCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAA           <  SRR4302543.6339278/94‑1 (MQ=60)
                                                                                  CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCCATAAAAATAAAG          <  SRR4302543.7857957/94‑1 (MQ=60)
                                                                                  CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG          <  SRR4302543.2469655/94‑1 (MQ=60)
                                                                                  CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG          <  SRR4302543.527570/94‑1 (MQ=60)
                                                                                   GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT         <  SRR4302543.3751787/94‑1 (MQ=60)
                                                                                   GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGA         >  SRR4302543.5382429/1‑94 (MQ=60)
                                                                                    GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA        <  SRR4302543.2800112/94‑1 (MQ=60)
                                                                                    GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA        <  SRR4302543.4843569/94‑1 (MQ=60)
                                                                                     GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA       >  SRR4302543.288550/1‑94 (MQ=60)
                                                                                      CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT      <  SRR4302543.4673921/94‑1 (MQ=60)
                                                                                      CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT      <  SRR4302543.2585119/94‑1 (MQ=60)
                                                                                       GATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT     <  SRR4302543.1060169/94‑1 (MQ=60)
                                                                                        ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA    >  SRR4302543.6959198/1‑94 (MQ=60)
                                                                                        ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA    <  SRR4302543.6840586/94‑1 (MQ=60)
                                                                                        ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA    >  SRR4302543.6829740/1‑94 (MQ=60)
                                                                                         TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT   <  SRR4302543.6329845/94‑1 (MQ=60)
                                                                                         TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT   <  SRR4302543.295608/94‑1 (MQ=60)
                                                                                         TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT   <  SRR4302543.3998261/94‑1 (MQ=60)
                                                                                         TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT   >  SRR4302543.6073828/1‑94 (MQ=60)
                                                                                          AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA  >  SRR4302543.5371280/1‑94 (MQ=60)
                                                                                             |                                                                                           
CAGCTCGTCAAGATTCGGATCGGCGACGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA  >  NC_000913/4476948‑4477131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑