Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,478,503 (A)7→6 intergenic (+12/+47) yjgN → / ← valS hypothetical protein/valine tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,478,4970A.99.4% 1441.0 / 0.4 317intergenic (+6/+53)yjgN/valShypothetical protein/valine tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base . (165/150);  total (167/150)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.97e-01

Only 100 of 386 total aligned reads displayed.

CCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT  >  NC_000913/4478405‑4478587
                                                                                            |                                                                                          
  tttAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaa                                                                                          <  1:6538931/93‑1 (MQ=255)
   ttAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaa                                                                                         <  1:2308264/93‑1 (MQ=255)
    tAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaa                                                                                        >  1:5496126/1‑93 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                       <  1:3018107/93‑1 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                       <  1:4637115/93‑1 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                       >  1:3525464/1‑93 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                       >  2:574384/1‑93 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                       <  1:6343035/93‑1 (MQ=255)
     aaTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaa                                                                                       <  2:6308202/93‑1 (MQ=255)
      aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaag                                                                                      <  2:5815041/93‑2 (MQ=255)
      aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaag                                                                                      >  1:4771873/1‑92 (MQ=255)
      aTCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaag                                                                                      <  2:2153707/93‑2 (MQ=255)
       tCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaagg                                                                                     <  2:1691307/93‑3 (MQ=255)
       tCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaagg                                                                                     >  2:2194509/1‑91 (MQ=255)
        cAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggc                                                                                    <  1:4674362/93‑4 (MQ=255)
        cAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggc                                                                                    <  1:1497935/93‑4 (MQ=255)
        cAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggc                                                                                    <  1:7413530/93‑4 (MQ=255)
         aaTAAAGAAGAACAACCAGATAAAGGCTTCTTAGTCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggcc                                                                                   <  1:4833972/93‑5 (MQ=255)
         aaTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggcc                                                                                   <  1:5636110/93‑5 (MQ=255)
         aaTAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATaaaaaaggcc                                                                                   >  2:4311281/1‑89 (MQ=255)
            aaaGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGTGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                               >  1:4971103/1‑93 (MQ=255)
            aaaGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                               <  1:3149054/93‑1 (MQ=255)
            aaaGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                               <  1:7806484/93‑1 (MQ=255)
            aaaGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGa                                                                               <  2:1352602/93‑1 (MQ=255)
             aagaagAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGag                                                                              <  1:6174085/93‑2 (MQ=255)
              agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagc                                                                             <  1:4152873/93‑3 (MQ=255)
              agaagaACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCGTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagc                                                                             >  1:3417915/1‑91 (MQ=255)
               gaagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagca                                                                            <  1:7212047/93‑4 (MQ=255)
               gaagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagca                                                                            <  2:3896567/93‑4 (MQ=255)
                aagaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGagcat                                                                           <  1:50627/93‑5 (MQ=255)
                 agaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAATAGAT‑AAAAAAGGCCGga‑gcatg                                                                         <  2:6425940/93‑1 (MQ=255)
                  gaaCAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGCAAGAGAT‑AAAAAAGGCCGga‑gcatgc                                                                        <  1:5207484/93‑1 (MQ=255)
                    acaacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTc                                                                      <  2:1099249/93‑1 (MQ=255)
                    acaacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTc                                                                      <  2:2465526/93‑1 (MQ=255)
                      aacCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCg                                                                    >  2:126742/1‑93 (MQ=255)
                       acCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCgg                                                                   >  1:4207157/1‑93 (MQ=255)
                          aGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                <  2:2394401/93‑1 (MQ=255)
                          aGATAAAGGCTTCTTAGCCAGAATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCt                                                                >  2:4682806/1‑93 (MQ=255)
                           gATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCtt                                                               >  1:1118512/1‑93 (MQ=255)
                            aTAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttc                                                              >  1:6023395/1‑92 (MQ=255)
                               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                           >  1:4503878/1‑89 (MQ=255)
                               aaGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCttcgtt                                                           <  2:4447331/93‑5 (MQ=255)
                                 ggCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGtttt                                                        <  1:7709245/93‑1 (MQ=255)
                                 ggCTTCTTAACCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGtttt                                                        >  2:5244530/1‑93 (MQ=255)
                                  gCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtc                                                       >  2:6736234/1‑93 (MQ=255)
                                    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcat                                                     >  1:2394613/1‑93 (MQ=255)
                                    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcat                                                     >  2:2002944/1‑93 (MQ=255)
                                    ttcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcat                                                     >  1:2045704/1‑93 (MQ=255)
                                     tcttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatc                                                    >  2:4034892/1‑93 (MQ=255)
                                      tttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatca                                                   <  2:1552809/92‑1 (MQ=255)
                                      cttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTtcatca                                                   <  2:4921245/93‑1 (MQ=255)
                                       ttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTTTTCATCAc                                                  >  1:5945968/1‑93 (MQ=255)
                                       ttAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAc                                                  >  1:6880645/1‑93 (MQ=255)
                                         aGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtg                                                <  2:853483/93‑1 (MQ=255)
                                          gCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgt                                               >  2:2288292/1‑93 (MQ=255)
                                           ccAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACtgtg                                              >  2:4800918/1‑93 (MQ=255)
                                            cAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCGGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTgtgt                                             <  2:2858225/93‑1 (MQ=255)
                                            cAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTgtgt                                             >  1:923528/1‑93 (MQ=255)
                                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtt                                            <  2:3511064/93‑1 (MQ=255)
                                             aGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtt                                            >  1:3281507/1‑93 (MQ=255)
                                               tATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGtttt                                          <  1:4306363/93‑1 (MQ=255)
                                                aTTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTg                                         >  2:6774339/1‑93 (MQ=255)
                                                  ttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAt                                       <  2:6500967/93‑1 (MQ=255)
                                                  ttCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTTCATCACTGTGTTTTGAt                                       <  1:7893795/93‑1 (MQ=255)
                                                   tCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGAtt                                      <  1:2939816/93‑1 (MQ=255)
                                                    cACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTa                                     >  1:2247043/1‑93 (MQ=255)
                                                     aCGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTAc                                    <  1:1885969/93‑1 (MQ=255)
                                                     aCGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTAc                                    >  2:3590172/1‑93 (MQ=255)
                                                      cGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACa                                   <  1:7007029/93‑1 (MQ=255)
                                                       ggggAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAg                                  >  2:4109673/1‑93 (MQ=255)
                                                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                               <  1:371754/93‑1 (MQ=255)
                                                          gAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAgcgc                               >  1:7540724/1‑93 (MQ=255)
                                                           aGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGcgcg                              >  2:7514708/1‑93 (MQ=255)
                                                            gTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcgg                             >  1:709116/1‑93 (MQ=255)
                                                            gTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcgg                             >  1:1034462/1‑93 (MQ=255)
                                                             ttATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggc                            >  1:6800119/1‑93 (MQ=255)
                                                              tATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCgcggcg                           >  2:5491317/1‑93 (MQ=255)
                                                               aTGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGAGGCGa                          >  2:1769733/1‑93 (MQ=255)
                                                                tGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGAt                         >  1:4039645/1‑93 (MQ=255)
                                                                   cttcttTACCATTACTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAAc                      >  2:325716/1‑93 (MQ=255)
                                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                     <  1:5425580/93‑1 (MQ=255)
                                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                     >  1:7491038/1‑93 (MQ=255)
                                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                     >  2:5109837/1‑93 (MQ=255)
                                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                     >  2:1164677/1‑93 (MQ=255)
                                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                     <  1:5675531/93‑1 (MQ=255)
                                                                    ttcttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACa                     <  2:6140110/93‑1 (MQ=255)
                                                                     tcttAACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAg                    >  2:5048891/1‑93 (MQ=255)
                                                                      cttTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGc                   <  2:4523512/93‑1 (MQ=255)
                                                                       tttACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGcc                  <  1:156050/93‑1 (MQ=255)
                                                                          aCCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgc               >  1:1368440/1‑93 (MQ=255)
                                                                           ccATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCctgct              <  2:7239996/93‑1 (MQ=255)
                                                                               ttCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTc          <  1:1633952/93‑1 (MQ=255)
                                                                                tCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCa         >  2:423345/1‑93 (MQ=255)
                                                                                tCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCa         >  2:2638469/1‑93 (MQ=255)
                                                                                  tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAt       >  1:2568470/1‑93 (MQ=255)
                                                                                  tGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAt       >  2:6872552/1‑93 (MQ=255)
                                                                                   gTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATc      <  1:2698894/93‑1 (MQ=255)
                                                                                    tAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCa     >  1:3222934/1‑93 (MQ=255)
                                                                                       gagaT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtt  >  2:2566058/1‑93 (MQ=255)
                                                                                       gagaT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGtt  >  2:3787218/1‑93 (MQ=255)
                                                                                            |                                                                                          
CCTTTAATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTGTTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT  >  NC_000913/4478405‑4478587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT  >  NC_000913/4478410‑4478598
                                                                                             |                                                                                                 
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACTATTTCTGTAAGAGATAAAAAAG                                                                                                   >  SRR4302543.769599/1‑94 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   <  SRR4302543.6060048/94‑1 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   <  SRR4302543.3045398/94‑1 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   <  SRR4302543.6402678/94‑1 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   <  SRR4302543.4679287/94‑1 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   <  SRR4302543.6802955/94‑1 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   <  SRR4302543.3986385/94‑1 (MQ=60)
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAG                                                                                                   >  SRR4302543.4815481/1‑94 (MQ=60)
 ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGG                                                                                                  >  SRR4302543.65735/1‑94 (MQ=60)
 ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGG                                                                                                  <  SRR4302543.6998760/94‑1 (MQ=60)
 ATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAGG                                                                                                  <  SRR4302543.7620920/94‑1 (MQ=60)
  TCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGC                                                                                                >  SRR4302543.7253691/1‑94 (MQ=60)
  TCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGC                                                                                                >  SRR4302543.6045455/1‑94 (MQ=60)
  ACAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGC                                                                                                <  SRR4302543.4723596/94‑1 (MQ=60)
   CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                               <  SRR4302543.1511408/94‑1 (MQ=60)
   CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                               <  SRR4302543.7484656/94‑1 (MQ=60)
   CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                               <  SRR4302543.4716977/94‑1 (MQ=60)
   CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                               <  SRR4302543.1511407/94‑1 (MQ=60)
   CAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCC                                                                                               <  SRR4302543.854532/94‑1 (MQ=60)
    AATAAAGAAGAACAACCAGATAAAGGCTTCTTAGTCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCG                                                                                              <  SRR4302543.4878063/94‑1 (MQ=60)
    AATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCG                                                                                              <  SRR4302543.5687480/94‑1 (MQ=60)
    AATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCG                                                                                              <  SRR4302543.5062280/94‑1 (MQ=60)
     ATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG                                                                                             >  SRR4302543.5046806/1‑94 (MQ=60)
     ATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGG                                                                                             >  SRR4302543.4827593/1‑94 (MQ=60)
      TAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGTGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA                                                                                            >  SRR4302543.5016344/1‑94 (MQ=60)
      TAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA                                                                                            <  SRR4302543.6155068/94‑1 (MQ=60)
       AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGAG                                                                                           <  SRR4302543.3177380/94‑1 (MQ=60)
       AAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGAG                                                                                           <  SRR4302543.7880069/94‑1 (MQ=60)
         AGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCA                                                                                        <  SRR4302543.4190417/94‑1 (MQ=60)
          GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                       <  SRR4302543.7281958/94‑1 (MQ=60)
          GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                       <  SRR4302543.4362997/94‑1 (MQ=60)
          GAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAT                                                                                       <  SRR4302543.6939613/94‑1 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      <  SRR4302543.51077/94‑1 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      <  SRR4302543.5851321/94‑1 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      >  SRR4302543.324110/1‑94 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      <  SRR4302543.499614/94‑1 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      <  SRR4302543.804738/94‑1 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      <  SRR4302543.5168520/94‑1 (MQ=60)
           AAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTGTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATG                                                                                      <  SRR4302543.51078/94‑1 (MQ=60)
             GAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCT                                                                                    <  SRR4302543.3259052/94‑1 (MQ=60)
             GAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGCAAGAGAT‑AAAAAAGGCCGGA‑GCATGCT                                                                                    <  SRR4302543.5254416/94‑1 (MQ=60)
                CAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCG                                                                                 <  SRR4302543.2386234/94‑1 (MQ=60)
                 AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                >  SRR4302543.3324959/1‑94 (MQ=60)
                 AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                >  SRR4302543.3045050/1‑94 (MQ=60)
                 AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                >  SRR4302543.4245307/1‑94 (MQ=60)
                 AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                >  SRR4302543.4245304/1‑94 (MQ=60)
                 AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                <  SRR4302543.202262/94‑1 (MQ=60)
                 AACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGG                                                                                >  SRR4302543.7548799/1‑94 (MQ=60)
                  ACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGC                                                                               >  SRR4302543.2787167/1‑94 (MQ=60)
                   CCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCC                                                                              <  SRR4302543.5841993/94‑1 (MQ=60)
                    CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                             <  SRR4302543.6914350/94‑1 (MQ=60)
                    CAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCT                                                                             <  SRR4302543.4665266/94‑1 (MQ=60)
                     AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                            >  SRR4302543.1128648/1‑94 (MQ=60)
                     AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                            >  SRR4302543.4592758/1‑94 (MQ=60)
                     AGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT                                                                            >  SRR4302543.6580989/1‑94 (MQ=60)
                      GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCcggcctc                                                                           >  SRR4302543.7065514/1‑87 (MQ=60)
                      GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTC                                                                           <  SRR4302543.3157323/94‑1 (MQ=60)
                      GATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTC                                                                           >  SRR4302543.6079367/1‑94 (MQ=60)
                       ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                          >  SRR4302543.3664614/1‑92 (MQ=60)
                       ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                          >  SRR4302543.6256313/1‑92 (MQ=60)
                       ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                          >  SRR4302543.6024560/1‑92 (MQ=60)
                       ATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTTcg                                                                          >  SRR4302543.7868535/1‑92 (MQ=60)
                         AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                       >  SRR4302543.4544822/1‑94 (MQ=60)
                         AAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTT                                                                       >  SRR4302543.5198179/1‑94 (MQ=60)
                          AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                      <  SRR4302543.7375567/94‑1 (MQ=60)
                          AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                      <  SRR4302543.7375568/94‑1 (MQ=60)
                          AAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTT                                                                      >  SRR4302543.7486490/1‑94 (MQ=60)
                            GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                    >  SRR4302543.3302221/1‑94 (MQ=60)
                            GGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTC                                                                    <  SRR4302543.7782174/94‑1 (MQ=60)
                             GCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCA                                                                   <  SRR4302543.5553628/94‑1 (MQ=60)
                              CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                                  >  SRR4302543.3685315/1‑94 (MQ=60)
                              CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                                  >  SRR4302543.2064041/1‑94 (MQ=60)
                              CTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCAT                                                                  >  SRR4302543.2416278/1‑94 (MQ=60)
                               TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                 <  SRR4302543.7655265/94‑1 (MQ=60)
                               TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                 >  SRR4302543.4568323/1‑94 (MQ=60)
                               TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                 >  SRR4302543.2534502/1‑94 (MQ=60)
                               TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                 >  SRR4302543.5564945/1‑94 (MQ=60)
                               TTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGACGGA‑GCATGCTCCGGCCTT‑CGTTTTCATC                                                                 >  SRR4302543.778756/1‑94 (MQ=60)
                                 CTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GGATGCTCCGGCCTT‑CGTTTTCATCAC                                                               >  SRR4302543.6000385/1‑94 (MQ=60)
                                 CTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCAC                                                               >  SRR4302543.6945389/1‑94 (MQ=60)
                                   TAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTG                                                             <  SRR4302543.6161518/94‑1 (MQ=60)
                                   TAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTG                                                             >  SRR4302543.1082977/1‑94 (MQ=60)
                                     GCCAGTATGTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCAGGGCTT‑CGTTTTCATCACTGTG                                                           <  SRR4302543.6550399/94‑1 (MQ=60)
                                      CCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑T                                                        >  SRR4302543.931609/1‑94 (MQ=60)
                                       CAGTATTTCACGGGGATTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TT                                                       <  SRR4302543.4271807/94‑1 (MQ=60)
                                       CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TT                                                       >  SRR4302543.3311130/1‑94 (MQ=60)
                                       CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TT                                                       >  SRR4302543.255667/1‑94 (MQ=60)
                                       CAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCAAGCTTCGGGCTT‑CGTTTTCATCACTGTG‑‑TT                                                       >  SRR4302543.7265734/1‑94 (MQ=60)
                                        AGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTT                                                      <  SRR4302543.1964937/94‑1 (MQ=60)
                                         GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTT                                                     >  SRR4302543.725405/1‑94 (MQ=60)
                                         GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTT                                                     <  SRR4302543.2665719/94‑1 (MQ=60)
                                         GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTT                                                     >  SRR4302543.815565/1‑94 (MQ=60)
                                         GTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCCCCCGCCTT‑CGTTTTCATCACTTTG‑‑TTTT                                                     >  SRR4302543.1623667/1‑94 (MQ=60)
                                          TATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTG                                                    <  SRR4302543.4345486/94‑1 (MQ=60)
                                           ATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGA                                                   >  SRR4302543.7350134/1‑94 (MQ=60)
                                           ATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCCT‑CGTTTTTATCACTGTGGTTTTT                                                     >  SRR4302543.1556205/1‑94 (MQ=59)
                                            TTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGAT                                                  >  SRR4302543.6671339/1‑94 (MQ=60)
                                             TTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATT                                                 <  SRR4302543.7968027/94‑1 (MQ=60)
                                              TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGtttc                                                >  SRR4302543.2267436/1‑90 (MQ=60)
                                              TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTA                                                >  SRR4302543.2267440/1‑94 (MQ=60)
                                              TCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTA                                                <  SRR4302543.2966512/94‑1 (MQ=60)
                                               TACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTAC                                               <  SRR4302543.2337415/94‑1 (MQ=60)
                                               CACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTAC                                               <  SRR4302543.1125456/94‑1 (MQ=60)
                                                ACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACA                                              <  SRR4302543.1902919/94‑1 (MQ=60)
                                                ACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACA                                              <  SRR4302543.1902918/94‑1 (MQ=60)
                                                 CGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAG                                             <  SRR4302543.7073695/94‑1 (MQ=60)
                                                 CGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAG                                             >  SRR4302543.5491982/1‑94 (MQ=60)
                                                 CGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAG                                             <  SRR4302543.1453279/94‑1 (MQ=60)
                                                 CGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGACTT‑CGTTTTCATCACTGTG‑‑TTTTGATTAtac                                             >  SRR4302543.430954/1‑91 (MQ=60)
                                                  GGTGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGC                                            <  SRR4302543.2832431/94‑1 (MQ=60)
                                                  GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGC                                            <  SRR4302543.4159210/94‑1 (MQ=60)
                                                  GGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGC                                            <  SRR4302543.4115250/94‑1 (MQ=60)
                                                   GGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCG                                           >  SRR4302543.492961/1‑94 (MQ=60)
                                                    GGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGC                                          <  SRR4302543.1238323/94‑1 (MQ=60)
                                                    GGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGC                                          >  SRR4302543.3507261/1‑94 (MQ=60)
                                                    GGAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGC                                          >  SRR4302543.7612594/1‑94 (MQ=60)
                                                     gatttcgacattcttaactattttTGTAAGAGATAAAAAA‑‑GCCGGGAGCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCG                                         <  SRR4302543.5671617/70‑1 (MQ=56)
                                                     GAGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCG                                         <  SRR4302543.375181/94‑1 (MQ=60)
                                                      AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGG                                        <  SRR4302543.3542696/94‑1 (MQ=60)
                                                      AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGG                                        >  SRR4302543.4763847/1‑94 (MQ=60)
                                                      AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGG                                        >  SRR4302543.715560/1‑94 (MQ=60)
                                                      AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGG                                        >  SRR4302543.3075525/1‑94 (MQ=60)
                                                      AGTTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGG                                        >  SRR4302543.1043646/1‑94 (MQ=60)
                                                        TTATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCG                                      >  SRR4302543.3432347/1‑94 (MQ=60)
                                                          ATGCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGAT                                    >  SRR4302543.4075801/1‑94 (MQ=60)
                                                            TCCTTCTTTACCATTTCTGTAAGAAAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAA                                  <  SRR4302543.1627263/94‑1 (MQ=60)
                                                            GCCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAA                                  >  SRR4302543.623143/1‑94 (MQ=60)
                                                             CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAAC                                 <  SRR4302543.5541716/94‑1 (MQ=60)
                                                             CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAAC                                 <  SRR4302543.2303542/94‑1 (MQ=60)
                                                             CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAAC                                 <  SRR4302543.4691270/94‑1 (MQ=60)
                                                             CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAAC                                 >  SRR4302543.2279625/1‑94 (MQ=60)
                                                             CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAAC                                 <  SRR4302543.4707016/94‑1 (MQ=60)
                                                             CCTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAAC                                 >  SRR4302543.4709099/1‑94 (MQ=60)
                                                              CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACA                                <  SRR4302543.4542350/94‑1 (MQ=60)
                                                              CTTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACA                                >  SRR4302543.7562708/1‑94 (MQ=60)
                                                               TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAG                               <  SRR4302543.5727340/94‑1 (MQ=60)
                                                               TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAG                               <  SRR4302543.5308099/94‑1 (MQ=60)
                                                               TTCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAG                               <  SRR4302543.5474893/94‑1 (MQ=60)
                                                               TTCTTTACCATTTCGGTGAGAAAT‑AAAAAAGGACGGA‑GCATCCGCCGGCGTT‑CGTTTTCATCACTGTG‑‑TTTTGAGTACAGCGCGGCGATAACAG                               <  SRR4302543.5308098/94‑1 (MQ=27)
                                                                TCTTTACTATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGC                              <  SRR4302543.4316848/94‑1 (MQ=60)
                                                                TCTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTTATCACTGTG‑‑GTTTGATTTCAGCGCGGCGATAACAGC                              >  SRR4302543.3184460/1‑94 (MQ=60)
                                                                 CTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCC                             >  SRR4302543.7491357/1‑94 (MQ=60)
                                                                 CTTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCC                             >  SRR4302543.1472665/1‑94 (MQ=60)
                                                                  TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCT                            >  SRR4302543.3247717/1‑94 (MQ=60)
                                                                  TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCT                            <  SRR4302543.7143918/94‑1 (MQ=60)
                                                                  TTTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCT                            <  SRR4302543.157393/94‑1 (MQ=60)
                                                                   TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTG                           <  SRR4302543.5601175/94‑1 (MQ=60)
                                                                   TTACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTG                           >  SRR4302543.533710/1‑94 (MQ=60)
                                                                    TACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGC                          >  SRR4302543.1380716/1‑94 (MQ=60)
                                                                     ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCT                         >  SRR4302543.4223059/1‑94 (MQ=60)
                                                                     ACCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCT                         >  SRR4302543.2405375/1‑94 (MQ=60)
                                                                     ACCATTTCTGTAAGAGAT‑‑AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGGTTACAGCGCGGCGATAACAGCCTGCTG                        >  SRR4302543.529476/1‑94 (MQ=60)
                                                                     ACCATGTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCT                         <  SRR4302543.7123111/94‑1 (MQ=60)
                                                                      CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTG                        >  SRR4302543.2673367/1‑94 (MQ=60)
                                                                      CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTG                        >  SRR4302543.1194441/1‑94 (MQ=60)
                                                                      CCATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCCGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTT                        >  SRR4302543.1631647/1‑94 (MQ=60)
                                                                      CCATTTCTGTAAGAGAT‑AAAAAAGGCCGG‑GGCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGGTAAAAGCCTGCTG                        >  SRR4302543.1565043/1‑94 (MQ=60)
                                                                       CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                       >  SRR4302543.1883518/1‑94 (MQ=60)
                                                                       CATTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGT                       >  SRR4302543.7610947/1‑94 (MQ=60)
                                                                         TTTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTC                     >  SRR4302543.7916894/1‑94 (MQ=60)
                                                                          TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                    <  SRR4302543.1648518/94‑1 (MQ=60)
                                                                          TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                    <  SRR4302543.3264840/94‑1 (MQ=60)
                                                                          TTCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCA                    >  SRR4302543.6568536/1‑94 (MQ=60)
                                                                           TCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAA                   >  SRR4302543.7883333/1‑94 (MQ=60)
                                                                           TCTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAA                   <  SRR4302543.3590494/94‑1 (MQ=60)
                                                                            CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                  >  SRR4302543.6964063/1‑94 (MQ=60)
                                                                            CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                  <  SRR4302543.2474081/94‑1 (MQ=60)
                                                                            CTGTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAAT                  >  SRR4302543.2591917/1‑94 (MQ=60)
                                                                              GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                >  SRR4302543.3252062/1‑94 (MQ=60)
                                                                              GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                <  SRR4302543.5695808/94‑1 (MQ=60)
                                                                              GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                >  SRR4302543.2160346/1‑94 (MQ=60)
                                                                              GTAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCA                <  SRR4302543.2723367/94‑1 (MQ=60)
                                                                               TAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG               >  SRR4302543.2273455/1‑94 (MQ=60)
                                                                               TAAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAG               <  SRR4302543.6360175/94‑1 (MQ=60)
                                                                                AAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGT              >  SRR4302543.3042007/1‑94 (MQ=60)
                                                                                AAGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGT              <  SRR4302543.4856266/94‑1 (MQ=60)
                                                                                 AGAGAT‑AAAAAAGTCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT             <  SRR4302543.6824478/94‑1 (MQ=60)
                                                                                 AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT             >  SRR4302543.7584783/1‑94 (MQ=60)
                                                                                 AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT             >  SRR4302543.2646344/1‑94 (MQ=60)
                                                                                 AGAGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTT             >  SRR4302543.7102449/1‑94 (MQ=60)
                                                                                   AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC           >  SRR4302543.7543575/1‑94 (MQ=60)
                                                                                   AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC           >  SRR4302543.4684386/1‑94 (MQ=60)
                                                                                   AGAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTC           >  SRR4302543.4374253/1‑94 (MQ=60)
                                                                                    GAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCG          <  SRR4302543.7431962/94‑1 (MQ=60)
                                                                                    GAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCG          <  SRR4302543.7431961/94‑1 (MQ=60)
                                                                                    GAT‑AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCG          <  SRR4302543.4031860/94‑1 (MQ=60)
                                                                                       TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT        <  SRR4302543.5724588/94‑1 (MQ=60)
                                                                                       TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT        <  SRR4302543.904083/94‑1 (MQ=60)
                                                                                       TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCT        <  SRR4302543.4084923/94‑1 (MQ=60)
                                                                                       TAAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGAT        >  SRR4302543.5558072/1‑94 (MQ=60)
                                                                                        AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT       >  SRR4302543.4515426/1‑94 (MQ=60)
                                                                                        AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT       >  SRR4302543.4122802/1‑94 (MQ=60)
                                                                                        AAAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTT       <  SRR4302543.6502012/94‑1 (MQ=60)
                                                                                         AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT      <  SRR4302543.2198365/94‑1 (MQ=60)
                                                                                         AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT      <  SRR4302543.2198366/94‑1 (MQ=60)
                                                                                         AAAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTT      <  SRR4302543.5688006/94‑1 (MQ=60)
                                                                                          AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC     >  SRR4302543.3096466/1‑94 (MQ=60)
                                                                                          AAAAGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTC     <  SRR4302543.333939/94‑1 (MQ=60)
                                                                                            AAGGCCGGA‑GCATGCTCTGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGC   <  SRR4302543.6260684/94‑1 (MQ=60)
                                                                                             AGGCCGGA‑GCATGCTCCGGCCTT‑CGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT  <  SRR4302543.2455582/94‑1 (MQ=60)
                                                                                             |                                                                                                 
AATCAATAAAGAAGAACAACCAGATAAAGGCTTCTTAGCCAGTATTTCACGGGGAGTTATGCCTTCTTTACCATTTCTGTAAGAGATAAAAAAAGGCCGGATGCATGCTCCGGCCTTGCGTTTTCATCACTGTG‑‑TTTTGATTACAGCGCGGCGATAACAGCCTGCTGTTCAATCAGTTTCGCTTTCGCT  >  NC_000913/4478410‑4478598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑