Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,924,185 A→G G14G (GGA→GGG)  b1844 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,924,1850AG99.2% 402.6 / NA 129G14G (GGA→GGG) b1844hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (77/51);  minor base T (0/1);  total (77/52)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

AAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAG  >  NC_000913/1924095‑1924274
                                                                                          |                                                                                         
aaGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggggg                                                                                         >  1:1280834/1‑93 (MQ=255)
aaGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggggg                                                                                         <  1:1356209/93‑1 (MQ=255)
 aGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCaggggggg                                                                                        >  2:3518950/1‑93 (MQ=255)
  ggCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCTATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAggggggga                                                                                       <  2:4439999/93‑1 (MQ=255)
     gATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCg                                                                                    >  1:3750551/1‑93 (MQ=255)
     gATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCg                                                                                    >  2:4455215/1‑93 (MQ=255)
     gATATCATACCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCg                                                                                    >  2:4545076/1‑93 (MQ=255)
      atatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGt                                                                                   >  1:3238052/1‑93 (MQ=255)
      atatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGt                                                                                   >  1:3122100/1‑93 (MQ=255)
       tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGtt                                                                                  <  2:1531221/93‑1 (MQ=255)
       tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGtt                                                                                  >  1:829481/1‑93 (MQ=255)
       tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGtt                                                                                  <  1:3565460/93‑1 (MQ=255)
          cATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTgag                                                                               >  2:3280341/1‑93 (MQ=255)
            ttCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGAt                                                                             <  1:3613464/93‑1 (MQ=255)
             tCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGAtt                                                                            <  1:3170157/93‑1 (MQ=255)
             tCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGAtt                                                                            <  2:2395565/93‑1 (MQ=255)
             tCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGAtt                                                                            <  1:1120702/93‑1 (MQ=255)
              ccATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGGTGAGATTg                                                                           >  2:3261157/1‑93 (MQ=255)
               cATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGc                                                                          >  2:3254477/1‑93 (MQ=255)
                aTTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGcc                                                                         >  2:1379181/1‑93 (MQ=255)
                aTTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGcc                                                                         >  1:592270/1‑93 (MQ=255)
                 ttACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCct                                                                        >  1:848621/1‑93 (MQ=255)
                  tACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCctc                                                                       <  2:200205/93‑1 (MQ=255)
                  tACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCctc                                                                       <  2:2596863/93‑1 (MQ=255)
                   aCGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCctct                                                                      <  1:690547/93‑1 (MQ=255)
                   aCGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCctct                                                                      <  1:2126606/93‑1 (MQ=255)
                   aCGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGCTTGCAGCGGGGGATCGTTGAGATTGCctct                                                                      >  1:1476495/1‑93 (MQ=255)
                     gCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGt                                                                    <  1:248330/93‑1 (MQ=255)
                     gCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGt                                                                    >  1:4610009/1‑93 (MQ=255)
                      cTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGtt                                                                   <  2:1303598/93‑1 (MQ=255)
                       tAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTg                                                                  >  2:2739816/1‑93 (MQ=255)
                        aGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGa                                                                 >  1:3247049/1‑93 (MQ=255)
                         ggCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGAt                                                                >  1:1728428/1‑93 (MQ=255)
                         ggCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGAt                                                                <  2:3371252/93‑1 (MQ=255)
                          gCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATg                                                               <  1:143916/93‑1 (MQ=255)
                          gCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATg                                                               >  2:805875/1‑93 (MQ=255)
                          gCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATg                                                               >  2:1792499/1‑93 (MQ=255)
                          gCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATg                                                               <  1:4320463/93‑1 (MQ=255)
                          gCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATg                                                               >  2:2011278/1‑93 (MQ=255)
                            tttttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTc                                                             >  1:739730/1‑93 (MQ=255)
                            tttttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTc                                                             <  1:3992835/93‑1 (MQ=255)
                             ttttCGGCCTGGAGCATGCTATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCa                                                            <  2:1027873/93‑1 (MQ=255)
                              tttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAt                                                           <  1:1490815/93‑1 (MQ=255)
                               ttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAtt                                                          <  1:1409020/93‑1 (MQ=255)
                               ttCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCAtt                                                          <  2:2347688/93‑1 (MQ=255)
                               ttCGCCATGGAGCATGCCATGTTGCGCATTATCGATACAGAAACTTGCGGTTTGCAGGGTGGGATCGTTGAGATTGCCTCTGTTGATGTCAtt                                                          <  2:2234362/93‑1 (MQ=255)
                                tCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTg                                                         >  2:2725193/1‑93 (MQ=255)
                                tCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTg                                                         <  2:2993027/93‑1 (MQ=255)
                                  ggCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGAc                                                       <  2:4355914/93‑1 (MQ=255)
                                   gCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACg                                                      >  2:3543702/1‑93 (MQ=255)
                                     cTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTTACGGa                                                    >  2:4262095/1‑93 (MQ=255)
                                     cTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGa                                                    >  1:1982508/1‑93 (MQ=255)
                                     cTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGa                                                    >  1:4406811/1‑93 (MQ=255)
                                      tGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGTGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGCCGGaa                                                   >  2:4352525/1‑93 (MQ=255)
                                       ggAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaa                                                  <  1:2891783/93‑1 (MQ=255)
                                       ggAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaa                                                  >  2:1412541/1‑93 (MQ=255)
                                         aGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTTCCTCTGTTGATGTCATTGACGGaaaaa                                                <  1:3949136/93‑1 (MQ=255)
                                         aGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaaa                                                <  1:1219169/93‑1 (MQ=255)
                                         aGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaaa                                                >  1:1405845/1‑93 (MQ=255)
                                           cATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATc                                              <  2:4231962/93‑1 (MQ=255)
                                            aTGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGGTGAGATTGGCTCTGTTGATGTCATTGACGGAAAAATCg                                             >  2:3400289/1‑93 (MQ=255)
                                             tGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGt                                            >  2:3862578/1‑93 (MQ=255)
                                              gCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTc                                           <  1:1152539/93‑1 (MQ=255)
                                               ccATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                                          <  2:1123100/93‑1 (MQ=255)
                                               ccATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                                          <  1:4279415/93‑1 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                                         >  1:2396437/1‑93 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                                         >  1:2274299/1‑93 (MQ=255)
                                                cATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCaa                                         >  1:3248672/1‑93 (MQ=255)
                                                     tGCGCATTATCTATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCa                                    <  2:1972408/93‑1 (MQ=255)
                                                     tGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCa                                    >  1:2295069/1‑93 (MQ=255)
                                                     tGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCa                                    >  2:1826696/1‑93 (MQ=255)
                                                       cgcATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATg                                  <  2:3405493/93‑1 (MQ=255)
                                                       cgcATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATg                                  >  1:280932/1‑93 (MQ=255)
                                                       cgcATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATg                                  <  1:3955171/93‑1 (MQ=255)
                                                        gcATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGa                                 <  1:4266512/93‑1 (MQ=255)
                                                         cATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAg                                <  1:3765629/93‑1 (MQ=255)
                                                          aTTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGc                               >  1:2705122/1‑93 (MQ=255)
                                                           ttATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc                              >  2:1554027/1‑93 (MQ=255)
                                                             aTCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAc                            >  1:558025/1‑93 (MQ=255)
                                                               cGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCt                          >  1:1939829/1‑93 (MQ=255)
                                                                gATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg                         >  1:1768350/1‑93 (MQ=255)
                                                                gATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg                         >  2:2949911/1‑93 (MQ=255)
                                                                gATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg                         <  1:172406/93‑1 (MQ=255)
                                                                gATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg                         >  1:3771899/1‑93 (MQ=255)
                                                                  tACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt                       >  1:3019628/1‑93 (MQ=255)
                                                                    cAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgc                     <  2:249569/93‑1 (MQ=255)
                                                                    cAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgc                     <  2:3905314/93‑1 (MQ=255)
                                                                      gAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTgcgc                   >  1:2641018/1‑93 (MQ=255)
                                                                       aaaCCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGcc                  <  2:2262217/93‑1 (MQ=255)
                                                                       aaaCCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGcc                  <  2:4231000/93‑1 (MQ=255)
                                                                         aCCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGcccc                >  1:3097114/1‑93 (MQ=255)
                                                                         aCCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGcccc                >  2:3882007/1‑93 (MQ=255)
                                                                           cTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGa              >  2:1360492/1‑93 (MQ=255)
                                                                            tGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGAt             >  1:3025243/1‑93 (MQ=255)
                                                                            tGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGAt             >  1:2426494/1‑93 (MQ=255)
                                                                            tGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGAt             >  2:1266090/1‑93 (MQ=255)
                                                                                gTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTc         <  1:2642511/93‑1 (MQ=255)
                                                                                gTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTc         >  1:1159000/1‑93 (MQ=255)
                                                                                  ttGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCt       <  2:1486098/93‑1 (MQ=255)
                                                                                       gggggggaTCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAg  <  2:885421/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATTAG  >  NC_000913/1924095‑1924274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATT  >  NC_000913/1924092‑1924272
                                                                                             |                                                                                       
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGG                                                                                         <  SRR4302535.557173/94‑1 (MQ=60)
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGG                                                                                         <  SRR4302535.1395620/94‑1 (MQ=60)
 GCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGG                                                                                        <  SRR4302535.1450429/94‑1 (MQ=60)
  CAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGG                                                                                       >  SRR4302535.1289976/1‑94 (MQ=60)
   AAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGG                                                                                      <  SRR4302535.1365914/94‑1 (MQ=60)
       CGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCG                                                                                  >  SRR4302535.3776516/1‑94 (MQ=60)
       CGATATCATTCCATTACGCTAGGC‑TTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGT                                                                                 >  SRR4302535.1690532/1‑94 (MQ=60)
        GATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGT                                                                                 >  SRR4302535.3143843/1‑94 (MQ=60)
        GATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGT                                                                                 >  SRR4302535.3260532/1‑94 (MQ=60)
         ATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTT                                                                                >  SRR4302535.835291/1‑94 (MQ=60)
          TATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTG                                                                               <  SRR4302535.3590183/94‑1 (MQ=60)
              ATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGGTCGTTGAGAT                                                                           >  SRR4302535.4192113/1‑94 (MQ=60)
              ATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGAT                                                                           <  SRR4302535.1876536/94‑1 (MQ=60)
               TTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATT                                                                          <  SRR4302535.3638517/94‑1 (MQ=60)
               TTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATT                                                                          >  SRR4302535.1220048/1‑94 (MQ=60)
                TCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTG                                                                         <  SRR4302535.3192111/94‑1 (MQ=60)
                TCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTG                                                                         <  SRR4302535.1128694/94‑1 (MQ=60)
                  CATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCC                                                                       >  SRR4302535.596421/1‑94 (MQ=60)
                   ATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCT                                                                      <  SRR4302535.2345586/94‑1 (MQ=60)
                   ATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCT                                                                      >  SRR4302535.854590/1‑94 (MQ=60)
                     TACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGCTTGCAGCGGGGGATCGTTGAGATTGCCTCT                                                                    >  SRR4302535.1487064/1‑94 (MQ=60)
                      ACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTG                                                                   <  SRR4302535.2141938/94‑1 (MQ=60)
                      ACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTG                                                                   <  SRR4302535.695387/94‑1 (MQ=60)
                       CGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGT                                                                  >  SRR4302535.4641331/1‑94 (MQ=60)
                        GCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTT                                                                 <  SRR4302535.250040/94‑1 (MQ=60)
                          TAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGA                                                               >  SRR4302535.3269598/1‑94 (MQ=60)
                           AGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGAT                                                              >  SRR4302535.1740920/1‑94 (MQ=60)
                           AGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGAT                                                              >  SRR4302535.3896383/1‑94 (MQ=60)
                             GCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGT                                                            <  SRR4302535.4350018/94‑1 (MQ=60)
                             GCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGT                                                            <  SRR4302535.144918/94‑1 (MQ=60)
                              CTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTC                                                           >  SRR4302535.744929/1‑94 (MQ=60)
                              CTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTC                                                           >  SRR4302535.4310236/1‑94 (MQ=60)
                              CTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGGTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTC                                                           >  SRR4302535.1373686/1‑94 (MQ=60)
                               TTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCA                                                          <  SRR4302535.4020148/94‑1 (MQ=60)
                                 TTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATT                                                        <  SRR4302535.1501520/94‑1 (MQ=60)
                                  TTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTG                                                       <  SRR4302535.1419082/94‑1 (MQ=60)
                                   TCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGA                                                      <  SRR4302535.3106684/94‑1 (MQ=60)
                                       CCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGA                                                  >  SRR4302535.1996782/1‑94 (MQ=60)
                                       CCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGA                                                  >  SRR4302535.4436970/1‑94 (MQ=60)
                                         TGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAA                                                >  SRR4302535.2952480/1‑94 (MQ=60)
                                          GGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAA                                               <  SRR4302535.2912241/94‑1 (MQ=60)
                                           GAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAA                                              >  SRR4302535.1415892/1‑94 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTTCCTCTGTTGATGTCATTGACGGAAAAAT                                             <  SRR4302535.3976100/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                             <  SRR4302535.852493/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                             <  SRR4302535.1227916/94‑1 (MQ=60)
                                            AGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAT                                             >  SRR4302535.2039394/1‑94 (MQ=60)
                                                 GCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCA                                        <  SRR4302535.1160773/94‑1 (MQ=60)
                                                  CCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                       <  SRR4302535.620205/94‑1 (MQ=60)
                                                  CCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                       >  SRR4302535.3271240/1‑94 (MQ=60)
                                                  CCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                       >  SRR4302535.2290532/1‑94 (MQ=60)
                                                  CCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                       <  SRR4302535.4308699/94‑1 (MQ=60)
                                                  CCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                       >  SRR4302535.3722883/1‑94 (MQ=60)
                                                  CCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAA                                       >  SRR4302535.2413661/1‑94 (MQ=60)
                                                       TTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCA                                  >  SRR4302535.2311485/1‑94 (MQ=60)
                                                         GCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATG                                >  SRR4302535.282892/1‑94 (MQ=60)
                                                          CGCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGA                               <  SRR4302535.3982178/94‑1 (MQ=60)
                                                           GCATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAG                              <  SRR4302535.4295725/94‑1 (MQ=60)
                                                            CATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGC                             >  SRR4302535.2724453/1‑94 (MQ=60)
                                                            CATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGC                             <  SRR4302535.3791637/94‑1 (MQ=60)
                                                            CATTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGC                             >  SRR4302535.3356126/1‑94 (MQ=60)
                                                              TTATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCA                           >  SRR4302535.3259620/1‑94 (MQ=60)
                                                               TATCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAC                          >  SRR4302535.561930/1‑94 (MQ=60)
                                                                 TCGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCT                        >  SRR4302535.1953802/1‑94 (MQ=60)
                                                                  CGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTG                       >  SRR4302535.3797919/1‑94 (MQ=60)
                                                                  CGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTG                       >  SRR4302535.1422165/1‑94 (MQ=60)
                                                                  CGATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTG                       >  SRR4302535.1781171/1‑94 (MQ=60)
                                                                   GATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGG                      <  SRR4302535.173601/94‑1 (MQ=60)
                                                                    ATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTTTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCAACTGGT                     >  SRR4302535.47047/1‑94 (MQ=60)
                                                                    ATACAGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGT                     >  SRR4302535.3040776/1‑94 (MQ=60)
                                                                        AGAAACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGC                 >  SRR4302535.2659937/1‑94 (MQ=60)
                                                                           AACCTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCC              >  SRR4302535.3118679/1‑94 (MQ=60)
                                                                              CTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGAT           >  SRR4302535.3046421/1‑94 (MQ=60)
                                                                              CTGCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGAT           >  SRR4302535.2443977/1‑94 (MQ=60)
                                                                                GCGGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCG         <  SRR4302535.2673987/94‑1 (MQ=60)
                                                                                  GGTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTC       >  SRR4302535.1167286/1‑94 (MQ=60)
                                                                                   GTTTGCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCC      <  SRR4302535.2661436/94‑1 (MQ=60)
                                                                                       GCAGGGGGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATT  <  SRR4302535.2581044/94‑1 (MQ=60)
                                                                                             |                                                                                       
GGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCGCCCCGATCGTCCTATT  >  NC_000913/1924092‑1924272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑