Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,008,480 +A coding (637/654 nt) yedK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,008,4761.A99.1% 404.4 / ‑3.6 114coding (633/654 nt)yedKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (40/73);  total (40/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 129 total aligned reads displayed.

AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563
                                                                                             |                                                                                       
aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGtt                                                                                          <  2:3772842/93‑1 (MQ=255)
 gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a                                                                                        >  1:1145619/1‑93 (MQ=255)
 gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑a                                                                                        >  1:3801409/1‑93 (MQ=255)
  cGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aa                                                                                       <  1:4327931/93‑1 (MQ=255)
  cGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aa                                                                                       <  1:936802/93‑1 (MQ=255)
   ggTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTTGGTAATGTT‑aaa                                                                                      >  1:3535708/1‑93 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aaaaa                                                                                    <  1:4209393/93‑2 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aaaaa                                                                                    >  2:1087988/1‑92 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aaaaa                                                                                    >  2:3672585/1‑92 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aaaaa                                                                                    >  1:3150719/1‑92 (MQ=255)
      aaaGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑aaaaac                                                                                   >  1:3778691/1‑91 (MQ=255)
        agaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATGGCGCGCGGTGGGTGATGTT‑aaaaacca                                                                                 >  2:661347/1‑89 (MQ=255)
          aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAgg                                                                                <  2:202111/93‑1 (MQ=255)
           agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAggg                                                                               <  2:2721021/93‑1 (MQ=255)
           agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAggg                                                                               <  2:2334255/93‑1 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGgcgc                                                                            >  1:419836/1‑93 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGgcgc                                                                            >  1:338803/1‑93 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGcgcg                                                                           >  1:3254249/1‑93 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGCTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGcgcg                                                                           >  2:1601178/1‑93 (MQ=255)
                 aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGa                                                                         <  2:3290979/93‑1 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAg                                                                        >  1:708988/1‑93 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAg                                                                        >  2:2320169/1‑93 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGt                                                                       <  1:3707226/93‑1 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGt                                                                       >  2:1849778/1‑93 (MQ=255)
                    aaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGtt                                                                      >  1:3909709/1‑93 (MQ=255)
                      tCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTaa                                                                    >  2:744335/1‑93 (MQ=255)
                      tCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTaa                                                                    >  2:3809161/1‑93 (MQ=255)
                       cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAAt                                                                   <  2:1320720/93‑1 (MQ=255)
                       cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAAt                                                                   <  1:1775852/93‑1 (MQ=255)
                        gcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAAtt                                                                  <  1:3004784/93‑1 (MQ=255)
                         cgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTc                                                                 >  1:4140378/1‑93 (MQ=255)
                          ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCa                                                                >  2:4019153/1‑93 (MQ=255)
                          ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCa                                                                >  2:4392253/1‑93 (MQ=255)
                            ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAAc                                                              <  1:1724083/93‑1 (MQ=255)
                            ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAAc                                                              <  1:2458732/93‑1 (MQ=255)
                             cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAAcc                                                             >  1:1552665/1‑93 (MQ=255)
                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGCTAATTCAACCt                                                            >  2:1561662/1‑93 (MQ=255)
                               gTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTg                                                           >  2:3242308/1‑93 (MQ=255)
                                tGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGt                                                          <  2:2005152/93‑1 (MQ=255)
                                 ggCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGtt                                                         <  2:80207/93‑1 (MQ=255)
                                 ggCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGtt                                                         <  1:1424967/93‑1 (MQ=255)
                                  gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGttt                                                        >  1:2035622/1‑93 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      <  1:3147703/93‑1 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      >  1:56007/1‑93 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      >  2:4220862/1‑93 (MQ=255)
                                     gtgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                                     <  2:3062146/93‑1 (MQ=255)
                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  1:4551805/93‑1 (MQ=255)
                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  2:4352932/93‑1 (MQ=255)
                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  2:3981728/93‑1 (MQ=255)
                                         tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGc                                                 <  1:1247467/93‑1 (MQ=255)
                                         tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGc                                                 <  2:476820/93‑1 (MQ=255)
                                         tCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGc                                                 <  1:3647828/93‑1 (MQ=255)
                                          ccAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGcc                                                <  1:1168774/93‑1 (MQ=255)
                                           cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                                               <  2:1824982/93‑1 (MQ=255)
                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              <  1:2759286/93‑1 (MQ=255)
                                            aGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTg                                              >  2:2652801/1‑93 (MQ=255)
                                             gCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                                             >  2:2348568/1‑93 (MQ=255)
                                              cAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGga                                            <  2:108390/93‑1 (MQ=255)
                                               aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgag                                           >  1:1550998/1‑93 (MQ=255)
                                                aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgaga                                          <  1:3191414/93‑1 (MQ=255)
                                                 aCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                         <  2:1754366/93‑1 (MQ=255)
                                                 aCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                         <  2:519871/93‑1 (MQ=255)
                                                   cAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGatta                                       <  2:2519836/93‑1 (MQ=255)
                                                    aGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattat                                      <  2:1248906/93‑1 (MQ=255)
                                                     gTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattatt                                     <  2:2754827/93‑1 (MQ=255)
                                                      ttCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTg                                    <  2:1108894/93‑1 (MQ=255)
                                                        ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAt                                  <  1:3918704/93‑1 (MQ=255)
                                                         tcCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATc                                 <  1:4055593/93‑1 (MQ=255)
                                                           cTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaa                               >  2:4466728/1‑93 (MQ=255)
                                                            tGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaac                              >  2:2101260/1‑93 (MQ=255)
                                                            tGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaac                              <  1:2420050/93‑1 (MQ=255)
                                                             ggCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaaca                             >  2:1614348/1‑93 (MQ=255)
                                                              gCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaacaa                            <  2:2808133/93‑1 (MQ=255)
                                                               cACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAAt                           <  2:1814184/93‑1 (MQ=255)
                                                                aCCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          <  1:4051261/93‑1 (MQ=255)
                                                                 cccGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAc                         <  1:1923934/93‑1 (MQ=255)
                                                                  ccGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACt                        >  1:198556/1‑93 (MQ=255)
                                                                    ggTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc                      >  1:2238067/1‑93 (MQ=255)
                                                                     gTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGCGGTAATTCAAACCGGTTGATAAGACTGGAGATTATTTATCAACAATACTGCg                     >  2:4045008/1‑93 (MQ=255)
                                                                     gTATCGCGCGCGGTGGGTAATGTTAAAAACCACGGCGCGGAAGTAATTTAAACTGTTTGATAAGCCTCGCAATTATTTATCAAAAATACTGCg                     >  2:3212303/1‑93 (MQ=255)
                                                                        tCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                  <  1:1939016/93‑1 (MQ=255)
                                                                        tCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                  >  1:3979235/1‑93 (MQ=255)
                                                                           cgcgcgGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATaa               <  1:4089991/93‑1 (MQ=255)
                                                                           cgcgcgGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATaa               <  1:4251017/93‑1 (MQ=255)
                                                                              gcgGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaa            <  2:1323152/93‑1 (MQ=255)
                                                                              gcgGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaa            >  1:585810/1‑93 (MQ=255)
                                                                               cgGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  2:1923249/93‑1 (MQ=255)
                                                                                ggTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAt          <  1:3710341/93‑1 (MQ=255)
                                                                                  tGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAATAAAtct        >  1:377622/1‑93 (MQ=255)
                                                                                  tGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtct        <  1:3525150/93‑1 (MQ=255)
                                                                                   gggTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       >  1:10798/1‑93 (MQ=255)
                                                                                   gggTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       <  1:3443297/93‑1 (MQ=255)
                                                                                   gggTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       <  1:866216/93‑1 (MQ=255)
                                                                                   gggTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       >  2:3933696/1‑93 (MQ=255)
                                                                                   gggTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       >  2:3886335/1‑93 (MQ=255)
                                                                                     gTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTTATAAGAAATCTCTa     <  1:3689083/93‑1 (MQ=255)
                                                                                     gTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTa     <  2:1295623/93‑1 (MQ=255)
                                                                                       aaTGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGAAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAtt   <  1:3306420/93‑1 (MQ=255)
                                                                                        aTGTTAAAAACCAGGGCGCGTAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  <  1:3513339/93‑1 (MQ=255)
                                                                                        aTGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  <  2:2432933/93‑1 (MQ=255)
                                                                                             |                                                                                       
AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGAT  >  NC_000913/2008388‑2008572
                                                                                             |                                                                                            
GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAAA                                                                                             >  SRR4302535.3172617/1‑94 (MQ=60)
 TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAAAC                                                                                            >  SRR4302535.3804734/1‑94 (MQ=60)
 TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAAAC                                                                                            <  SRR4302535.4238201/94‑1 (MQ=60)
    AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAG                                                                                          >  SRR4302535.4278626/1‑94 (MQ=60)
        GCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCG                                                                                      <  SRR4302535.637068/94‑1 (MQ=60)
         CCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGC                                                                                     >  SRR4302535.422772/1‑94 (MQ=60)
         CCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGC                                                                                     >  SRR4302535.341150/1‑94 (MQ=60)
          CTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCG                                                                                    >  SRR4302535.3276859/1‑94 (MQ=60)
             AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAG                                                                                 >  SRR4302535.713965/1‑94 (MQ=60)
               AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTT                                                                               <  SRR4302535.3732894/94‑1 (MQ=60)
               AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTT                                                                               >  SRR4302535.3936483/1‑94 (MQ=60)
                   CGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATT                                                                           <  SRR4302535.1788711/94‑1 (MQ=60)
                    GCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTC                                                                          <  SRR4302535.3025844/94‑1 (MQ=60)
                    GCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTC                                                                          >  SRR4302535.4168726/1‑94 (MQ=60)
                     CGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCA                                                                         <  SRR4302535.1970317/94‑1 (MQ=60)
                        CCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACC                                                                      <  SRR4302535.1736553/94‑1 (MQ=60)
                        CCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACC                                                                      <  SRR4302535.3504900/94‑1 (MQ=60)
                        CCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACC                                                                      <  SRR4302535.2476464/94‑1 (MQ=60)
                        CCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACC                                                                      >  SRR4302535.1563852/1‑94 (MQ=60)
                          AGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTG                                                                    <  SRR4302535.4338366/94‑1 (MQ=60)
                             GGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                 <  SRR4302535.1435140/94‑1 (MQ=60)
                             GGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                 >  SRR4302535.2050285/1‑94 (MQ=60)
                               CTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGA                                                               >  SRR4302535.56414/1‑94 (MQ=60)
                                TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGAT                                                              <  SRR4302535.3169586/94‑1 (MQ=60)
                                  TGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAA                                                            <  SRR4302535.3243023/94‑1 (MQ=60)
                                   GTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAG                                                           <  SRR4302535.4582912/94‑1 (MQ=60)
                                     TCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCC                                                         <  SRR4302535.3673087/94‑1 (MQ=60)
                                     TCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCC                                                         <  SRR4302535.1256367/94‑1 (MQ=60)
                                      CCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCT                                                        <  SRR4302535.1177116/94‑1 (MQ=60)
                                        AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                      <  SRR4302535.2778948/94‑1 (MQ=60)
                                          CAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAG                                                    >  SRR4302535.1562168/1‑94 (MQ=60)
                                            AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                  <  SRR4302535.3213491/94‑1 (MQ=60)
                                              CCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTA                                                <  SRR4302535.388248/94‑1 (MQ=60)
                                               CAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTAT                                               <  SRR4302535.1406937/94‑1 (MQ=60)
                                                  TTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGA                                            <  SRR4302535.2360152/94‑1 (MQ=60)
                                                    CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                          <  SRR4302535.3086013/94‑1 (MQ=60)
                                                    CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                          <  SRR4302535.3945508/94‑1 (MQ=60)
                                                     TCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCA                                         <  SRR4302535.4083361/94‑1 (MQ=60)
                                                        TGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACA                                      >  SRR4302535.1661922/1‑94 (MQ=60)
                                                        TGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACA                                      <  SRR4302535.2437475/94‑1 (MQ=60)
                                                           CACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATA                                   >  SRR4302535.405983/1‑94 (MQ=60)
                                                            ACCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                  <  SRR4302535.4078998/94‑1 (MQ=60)
                                                             CCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACT                                 <  SRR4302535.1937800/94‑1 (MQ=60)
                                                             CCCGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACT                                 >  SRR4302535.199919/1‑94 (MQ=60)
                                                               CGGTATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGC                               >  SRR4302535.2253991/1‑94 (MQ=60)
                                                                 GTATCGCGCGCAGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGT                             <  SRR4302535.3565620/94‑1 (MQ=60)
                                                                   ATCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                           >  SRR4302535.4006424/1‑94 (MQ=60)
                                                                    TCGCGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCAT                          <  SRR4302535.1952979/94‑1 (MQ=60)
                                                                       CGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                       <  SRR4302535.4117975/94‑1 (MQ=60)
                                                                       CGCGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAG                       <  SRR4302535.4280136/94‑1 (MQ=60)
                                                                         CGCGGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAA                     >  SRR4302535.589914/1‑94 (MQ=60)
                                                                            GGTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATC                  <  SRR4302535.3736041/94‑1 (MQ=60)
                                                                             GTGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAATAAATCT                 >  SRR4302535.380241/1‑94 (MQ=60)
                                                                              TGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTC                >  SRR4302535.10882/1‑94 (MQ=60)
                                                                              TGGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTC                <  SRR4302535.3549629/94‑1 (MQ=60)
                                                                               GGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT               <  SRR4302535.3467244/94‑1 (MQ=60)
                                                                               GGGTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT               <  SRR4302535.872341/94‑1 (MQ=60)
                                                                                 GTAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTTATAAGAAATCTCTAT             <  SRR4302535.3714616/94‑1 (MQ=60)
                                                                                  TAATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATT            <  SRR4302535.3792792/94‑1 (MQ=60)
                                                                                   AATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGAAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA           <  SRR4302535.3329428/94‑1 (MQ=60)
                                                                                    ATGTTAAAAACCAGGGCGCGTAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG          <  SRR4302535.3537751/94‑1 (MQ=60)
                                                                                    ATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG          >  SRR4302535.2774056/1‑94 (MQ=60)
                                                                                    ATGTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG          <  SRR4302535.4169723/94‑1 (MQ=60)
                                                                                       ttaAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA       <  SRR4302535.3635527/91‑1 (MQ=60)
                                                                                       ttaAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA       <  SRR4302535.2118021/91‑1 (MQ=60)
                                                                                        A‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAA     >  SRR4302535.2852761/1‑94 (MQ=60)
                                                                                          AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAG    >  SRR4302535.2024815/1‑94 (MQ=60)
                                                                                           AAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGA   <  SRR4302535.2765042/94‑1 (MQ=60)
                                                                                            AACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGAT  >  SRR4302535.630156/1‑94 (MQ=60)
                                                                                            AACCAGGGCGAGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATTTCTATTAGACAAAGAT  <  SRR4302535.628662/94‑1 (MQ=60)
                                                                                             |                                                                                            
GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGAT  >  NC_000913/2008388‑2008572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑